MX2020011083A - Proteina. - Google Patents

Proteina.

Info

Publication number
MX2020011083A
MX2020011083A MX2020011083A MX2020011083A MX2020011083A MX 2020011083 A MX2020011083 A MX 2020011083A MX 2020011083 A MX2020011083 A MX 2020011083A MX 2020011083 A MX2020011083 A MX 2020011083A MX 2020011083 A MX2020011083 A MX 2020011083A
Authority
MX
Mexico
Prior art keywords
xylanase
modified
parent
fragment
enzyme
Prior art date
Application number
MX2020011083A
Other languages
English (en)
Inventor
Igor Nikolaev
Bert Koops
Tuijl Jan Hendrik Van
Lorentsen Rikke Hoeegh
Lund Susan Arent
Original Assignee
Dupont Nutrition Biosci Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dupont Nutrition Biosci Aps filed Critical Dupont Nutrition Biosci Aps
Publication of MX2020011083A publication Critical patent/MX2020011083A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2485—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1,3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Animal Husbandry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)

Abstract

La presente invención se refiere a una enzima xilansa GH10 modificada o un fragmento de esta que tiene actividad xilanasa, en donde esa xilansa GH10 modificada o el fragmento de esta tiene unamayor termoestabilidad en comparación con una enzima xilansa GH10 parental; la xilansa GH10 parental se modificó en al menos dos posiciones siguientes 7, 33, 79, 217 y 298, en donde la numeración está basada en la numeración de aminoácidos de FveXyn4 (SEQ ID NO. 1). Particularmente, la enzima xilanasa modificada de conformidad con la presente invención tiene las modificaciones siguientes: N7D; T33V; K79Y, V, F, I, L o M (preferentemente, K79Y, F o V, con mayor preferencia, Y); A217Q, E, P, D o M (preferentemente, A217Q, E o P, con mayor preferencia, Q); y T298Y, F o W (preferentemente, Y o F, con mayor preferencia, Y).
MX2020011083A 2014-01-31 2016-07-25 Proteina. MX2020011083A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB201401648A GB201401648D0 (en) 2014-01-31 2014-01-31 Protein

Publications (1)

Publication Number Publication Date
MX2020011083A true MX2020011083A (es) 2020-11-06

Family

ID=50344157

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2016009651A MX2016009651A (es) 2014-01-31 2015-01-30 Proteina.
MX2020011083A MX2020011083A (es) 2014-01-31 2016-07-25 Proteina.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2016009651A MX2016009651A (es) 2014-01-31 2015-01-30 Proteina.

Country Status (16)

Country Link
US (1) US10165787B2 (es)
EP (2) EP3702450B1 (es)
JP (1) JP6522010B2 (es)
KR (2) KR102295417B1 (es)
CN (2) CN105992819B (es)
AR (1) AR101147A1 (es)
AU (2) AU2015212749B2 (es)
BR (1) BR112016017534A2 (es)
CA (1) CA2937043C (es)
DK (1) DK3099791T3 (es)
ES (1) ES2811767T3 (es)
GB (1) GB201401648D0 (es)
HU (1) HUE050893T2 (es)
MX (2) MX2016009651A (es)
PL (1) PL3099791T3 (es)
WO (1) WO2015114112A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11981942B2 (en) 2013-07-23 2024-05-14 International N&H Denmark Aps Xylanases for solubilizing arabinoxylan-containing material
BR112016017349A2 (pt) * 2014-01-31 2017-10-17 Danisco Us Inc métodos para melhorar os subprodutos de processos de fermentação usando xilanase
EP3394273A1 (en) 2015-12-22 2018-10-31 Novozymes A/S Process of extracting oil from thin stillage
WO2019121938A1 (en) * 2017-12-20 2019-06-27 Dsm Ip Assets B.V. Animal feed compositions comprising muramidase and uses thereof
CA3093378A1 (en) * 2018-03-09 2019-09-12 Danisco Us Inc Glucoamylases and methods of use thereof
CN112654703A (zh) 2018-07-06 2021-04-13 杜邦营养生物科学有限公司 用于基于谷类的动物饲料的含木聚糖酶饲料添加剂
CL2018003617A1 (es) 2018-12-14 2019-03-22 Univ Santiago Chile Polipéptido con actividad xilanasa, secuencia nucleotídica que lo codifica, ingrediente y proceso que comprende dicho ingrediente para la preparación de un producto alimenticio
US11440269B2 (en) * 2020-03-14 2022-09-13 Kurtis Zhang Process of making a gluten-based biodegradable material
KR20230167903A (ko) * 2022-06-03 2023-12-12 씨제이제일제당 (주) 자일라나제 활성을 갖는 변이형 폴리펩티드

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
ATE118545T1 (de) 1990-05-09 1995-03-15 Novo Nordisk As Eine ein endoglucanase enzym enthaltende zellulasezubereitung.
DK13491D0 (da) 1991-01-25 1991-01-25 Novo Nordisk As Anvendelse af et enzymholdigt granulat og fremgangsmaade til fremstilling af et forderstof i tabletform
BR9506262A (pt) * 1994-06-14 1997-08-12 Gist Brocades Bv Xilanase sequência de dna isolada e purificada vetor células hospedeira microbiana microorganismo isolado processos para a preparação de uma xilanase para a degradação de xilano para deslignificar pasta de madeira e produto obtido após o tratamento da pasta de madeira
AU7489896A (en) 1995-11-02 1997-05-22 Novo Nordisk A/S Feed enzyme preparations
FI108728B (fi) * 1999-10-12 2002-03-15 Carbozyme Oy Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
AU7779800A (en) 2000-09-21 2002-04-02 Dsm Nv Talaromyces xylanases
AU2002210409A1 (en) * 2000-11-10 2002-05-21 Novozymes A/S Ethanol process
US7510860B1 (en) 2001-11-21 2009-03-31 National Research Council Of Canada Xylanases with enhanced thermophilicity and alkalophilicity
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GB0612976D0 (en) * 2006-06-30 2006-08-09 Leuven K U Res & Dev Method for making arabinoxylo-oligosaccharides as a co-product of fermentation of whole grain cereals
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BR112016017349A2 (pt) * 2014-01-31 2017-10-17 Danisco Us Inc métodos para melhorar os subprodutos de processos de fermentação usando xilanase

Also Published As

Publication number Publication date
GB201401648D0 (en) 2014-03-19
AU2015212749A1 (en) 2016-07-07
KR20210094676A (ko) 2021-07-29
CN105992819B (zh) 2021-07-13
BR112016017534A2 (pt) 2017-12-12
CN113355307A (zh) 2021-09-07
US10165787B2 (en) 2019-01-01
EP3099791B1 (en) 2020-05-20
DK3099791T3 (da) 2020-08-24
AU2015212749A8 (en) 2016-08-25
WO2015114112A1 (en) 2015-08-06
HUE050893T2 (hu) 2021-01-28
PL3099791T3 (pl) 2020-11-16
WO2015114112A8 (en) 2016-08-11
AU2020203266A1 (en) 2020-06-11
EP3099791A1 (en) 2016-12-07
AR101147A1 (es) 2016-11-30
CA2937043C (en) 2023-10-24
KR102295417B1 (ko) 2021-08-31
MX2016009651A (es) 2016-11-28
EP3702450B1 (en) 2024-04-10
AU2015212749B2 (en) 2020-05-21
US20160345609A1 (en) 2016-12-01
KR102508272B1 (ko) 2023-03-08
AU2020203266B2 (en) 2022-09-15
CN105992819A (zh) 2016-10-05
EP3702450A1 (en) 2020-09-02
JP2017505636A (ja) 2017-02-23
CA2937043A1 (en) 2015-08-06
KR20160137955A (ko) 2016-12-02
JP6522010B2 (ja) 2019-05-29
ES2811767T3 (es) 2021-03-15

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