EA201300455A1 - Полипептиды с пермеазной активностью - Google Patents

Полипептиды с пермеазной активностью

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Publication number
EA201300455A1
EA201300455A1 EA201300455A EA201300455A EA201300455A1 EA 201300455 A1 EA201300455 A1 EA 201300455A1 EA 201300455 A EA201300455 A EA 201300455A EA 201300455 A EA201300455 A EA 201300455A EA 201300455 A1 EA201300455 A1 EA 201300455A1
Authority
EA
Eurasian Patent Office
Prior art keywords
activity
permaia
polypeptides
polypeptide
seq
Prior art date
Application number
EA201300455A
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English (en)
Inventor
Хендрик Ваутер Висселинк
Антониус Ерун Адриаан Ван Марис
Якобус Томас Пронк
Пол Классен
Рене Марсель Де Йонг
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201300455A1 publication Critical patent/EA201300455A1/ru

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Изобретение относится к полипептиду, имеющему мутацию в одной или нескольких позициях, соответствующих Т219 SEQ ID NO: 55, при этом полипептид имеет по меньшей мере 50% идентичность последовательности с SEQ ID NO: 55 и при этом полипептид обладает пермеазной активностью.
EA201300455A 2010-10-13 2011-10-11 Полипептиды с пермеазной активностью EA201300455A1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US39261710P 2010-10-13 2010-10-13
EP10075710 2010-10-13
PCT/EP2011/067726 WO2012049173A1 (en) 2010-10-13 2011-10-11 Polypeptides with permease activity

Publications (1)

Publication Number Publication Date
EA201300455A1 true EA201300455A1 (ru) 2013-09-30

Family

ID=43480439

Family Applications (2)

Application Number Title Priority Date Filing Date
EA201300455A EA201300455A1 (ru) 2010-10-13 2011-10-11 Полипептиды с пермеазной активностью
EA201300454A EA201300454A1 (ru) 2010-10-13 2011-10-11 Клетка дрожжей, сбраживающая пентозу и глюкозу

Family Applications After (1)

Application Number Title Priority Date Filing Date
EA201300454A EA201300454A1 (ru) 2010-10-13 2011-10-11 Клетка дрожжей, сбраживающая пентозу и глюкозу

Country Status (16)

Country Link
US (2) US9353376B2 (ru)
EP (2) EP2627765B1 (ru)
JP (2) JP2013542724A (ru)
KR (2) KR20140005883A (ru)
CN (2) CN103189505A (ru)
AR (3) AR083447A1 (ru)
AU (2) AU2011315569C1 (ru)
BR (2) BR112013009157A2 (ru)
CA (2) CA2813531A1 (ru)
DK (1) DK2627765T3 (ru)
EA (2) EA201300455A1 (ru)
ES (1) ES2629252T3 (ru)
MX (2) MX2013004127A (ru)
MY (1) MY160995A (ru)
PL (1) PL2627765T3 (ru)
WO (3) WO2012049179A2 (ru)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY160995A (en) 2010-10-13 2017-03-28 Dsm Ip Assets Bv Polypeptides with permease activity
WO2014145521A2 (en) 2013-03-15 2014-09-18 Alderbio Holdings Llc Fermentation process for antibody production
KR20160012153A (ko) * 2013-06-05 2016-02-02 디에스엠 아이피 어셋츠 비.브이. 투과효소 활성을 갖는 폴리펩타이드
WO2014195381A2 (en) * 2013-06-05 2014-12-11 Dsm Ip Assets B.V. Polypeptides with permease activity
WO2014195380A1 (en) * 2013-06-05 2014-12-11 Dsm Ip Assets B.V. Polypeptides with permease activity
WO2014195379A1 (en) * 2013-06-05 2014-12-11 Dsm Ip Assets B.V. Polypeptides with permease activity
WO2014195520A2 (en) * 2013-12-18 2014-12-11 Dsm Ip Assets B.V. Polypeptides with permease activity
WO2015091757A1 (en) * 2013-12-19 2015-06-25 Dsm Ip Assets B.V. Polypeptides with permease activity
KR102277898B1 (ko) 2014-07-03 2021-07-15 삼성전자주식회사 산물 생산능이 향상된 효모 및 그를 이용한 산물을 생산하는 방법
CN107074918B (zh) 2014-10-22 2021-07-30 布塔尔格有限公司 Gal2转运体的变异体和其用途
BR112018002284A2 (pt) 2015-08-05 2018-12-11 Cargill Inc método de fermentação para produzir um bioproduto, levedura crabtree negativa geneticamente engenheirada e método para reduzir a produção de glicerol por uma levedura engenheirada
US11208670B2 (en) 2015-10-22 2021-12-28 Basf Enzymes Llc Method for producing a fermentation product
BR122021020404B1 (pt) 2016-02-19 2022-09-20 Intercontinental Great Brands Llc Processo para criar múltiplas correntes de valor a partir de fontes de biomassa
CN106222101B (zh) * 2016-07-27 2020-03-27 中国科学技术大学 高温下高效利用菊芋一步生产高纯果糖的耐高温菌株及应用
WO2019055325A2 (en) * 2017-09-12 2019-03-21 Biocapital Holdings, Llc BIOOGICAL DEVICES AND METHODS OF USING THE SAME TO PRODUCE STEVIOL GLYCOSIDES
EP3710469B1 (en) * 2017-11-14 2021-12-08 DSM IP Assets B.V. Polypeptides with improved arabinose transport specificity
US11198847B2 (en) 2018-02-01 2021-12-14 Dsm Ip Assets B.V. Yeast cell capable of simultaneously fermenting hexose and pentose sugars
CN111304228B (zh) * 2020-03-18 2022-02-22 中国热带农业科学院橡胶研究所 一种橡胶树线粒体型己糖激酶基因及其编码蛋白和应用
WO2023000243A1 (zh) * 2021-07-22 2023-01-26 深圳先进技术研究院 重组酵母生物转化生产葡萄糖的方法

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014423A1 (en) 1989-05-18 1990-11-29 The Infergene Company Microorganism transformation
ES2109238T3 (es) 1989-07-07 1998-01-16 Unilever Nv Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
FR2679920A1 (fr) 1991-08-02 1993-02-05 Rhone Poulenc Rorer Sa Levures recombinantes hautement stables pour la production de proteines recombinantes, leur preparation et leur utilisation.
ES2198410T3 (es) 1993-07-23 2004-02-01 Dsm Ip Assets B.V. Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion.
DK0979294T3 (en) 1997-04-11 2015-08-31 Dsm Ip Assets Bv Gene conversion AS A TOOL FOR CONSTRUCTION OF RECOMBINANT INDUSTRIAL filamentous fungi
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
BR9910532A (pt) 1998-05-19 2001-10-16 Dsm Nv Produção melhorada in vivo de cefalosporina
CN1331751A (zh) 1998-12-22 2002-01-16 Dsm公司 改进的头孢菌素的体内生产方法
ATE418616T2 (de) * 2002-01-23 2009-01-15 Royal Nedalco B V Fermentation von pentosezuckern
SE0202090D0 (sv) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modifierd yeast consuming L-arabinose
PT1513923E (pt) 2002-05-30 2007-10-24 Natureworks Llc Métodos e materiais para a produção de ácido d-láctico em levedura
WO2004085627A1 (en) 2003-03-26 2004-10-07 Forskarpatent I Syd Ab New saccharomyces cerevisiae strains utilizing xylose
US7479385B2 (en) * 2003-12-05 2009-01-20 Wisconsin Alumni Research Foundation Sugar kinases with expanded substrate specificity and their use
CN101914462B (zh) 2004-07-16 2013-04-24 Dsm知识产权资产有限公司 发酵木糖的真核细胞的代谢工程
US7888489B2 (en) 2005-01-24 2011-02-15 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
EA016303B1 (ru) 2006-10-02 2012-04-30 ДСМ АйПи АССЕТС Б.В. Метаболическая инженерия сбраживающих арабинозу дрожжевых клеток
US8170326B2 (en) 2007-01-03 2012-05-01 Vialogy Corp. Multi parallax exploitation for omni-directional imaging electronic eye
US20100159546A1 (en) 2007-03-30 2010-06-24 Aristos Aristidou Metabolic engineering of yeasts for the production of 1-butanol
NZ598775A (en) * 2007-04-09 2014-03-28 Univ Georgia Substrate-selective co-fermentation process
CA2693365A1 (en) 2007-07-19 2009-01-22 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
ES2407639T3 (es) 2008-02-04 2013-06-13 Toray Industries, Inc. Procedimiento de producción de ácido láctico por fermentación continua
CN104099285A (zh) 2008-03-13 2014-10-15 帝斯曼知识产权资产管理有限公司 对能够发酵混合底物的生物的选择
DE102008029302B4 (de) 2008-06-19 2016-08-11 Insilico Biotechnology Ag Biotechnologische Herstellung von Acrylsäure
FR2934264B1 (fr) 2008-07-22 2012-07-20 Arkema France Fabrication d'esters de vinyle a partir de matieres renouvelables, esters de vinyle obtenus et utilisations
JP5821132B2 (ja) * 2008-12-24 2015-11-24 ディーエスエム アイピー アセッツ ビー.ブイ. キシロース異性化酵素遺伝子及びペントース糖の発酵におけるそれらの使用
CN102498202A (zh) 2009-02-25 2012-06-13 印第安纳大学研究和科技公司 鉴定和使用在d-葡萄糖存在下代谢戊糖的酵母菌株的材料和方法
EP2401378B1 (en) * 2009-02-25 2013-08-14 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
MY160995A (en) 2010-10-13 2017-03-28 Dsm Ip Assets Bv Polypeptides with permease activity

Also Published As

Publication number Publication date
AU2011315569A1 (en) 2013-04-11
AU2011315572A1 (en) 2013-04-11
MY160995A (en) 2017-03-28
ES2629252T3 (es) 2017-08-08
AU2011315569C1 (en) 2016-02-04
BR112013009157A2 (pt) 2016-07-26
CA2813531A1 (en) 2012-04-19
WO2012049173A1 (en) 2012-04-19
EP2627765A2 (en) 2013-08-21
JP2013542724A (ja) 2013-11-28
WO2012049179A2 (en) 2012-04-19
JP5961886B2 (ja) 2016-08-03
WO2012049170A3 (en) 2012-06-14
CN103154234B (zh) 2016-08-17
WO2012049179A3 (en) 2012-06-14
US9353376B2 (en) 2016-05-31
MX2013004127A (es) 2013-05-20
EA201300454A1 (ru) 2013-09-30
AR083449A1 (es) 2013-02-27
US20130236932A1 (en) 2013-09-12
KR20130100169A (ko) 2013-09-09
AU2011315569B2 (en) 2015-09-24
CA2813513A1 (en) 2012-04-19
AR083447A1 (es) 2013-02-27
KR20140005883A (ko) 2014-01-15
EP2627765B1 (en) 2017-05-10
WO2012049170A2 (en) 2012-04-19
JP2013539655A (ja) 2013-10-28
DK2627765T3 (en) 2017-08-28
US9034608B2 (en) 2015-05-19
AR083448A1 (es) 2013-02-27
AU2011315572B2 (en) 2015-09-03
PL2627765T3 (pl) 2017-10-31
BR112013008981A2 (pt) 2017-10-03
MX2013004121A (es) 2014-02-27
US20130273601A1 (en) 2013-10-17
EP2627767A1 (en) 2013-08-21
CN103189505A (zh) 2013-07-03
CN103154234A (zh) 2013-06-12

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