MX2017007503A - Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa. - Google Patents

Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa.

Info

Publication number
MX2017007503A
MX2017007503A MX2017007503A MX2017007503A MX2017007503A MX 2017007503 A MX2017007503 A MX 2017007503A MX 2017007503 A MX2017007503 A MX 2017007503A MX 2017007503 A MX2017007503 A MX 2017007503A MX 2017007503 A MX2017007503 A MX 2017007503A
Authority
MX
Mexico
Prior art keywords
sortase
seq
novel methods
enzyme mediated
polypeptide
Prior art date
Application number
MX2017007503A
Other languages
English (en)
Inventor
Kratzsch Peter
Boenitz-Dulat Mara
SCHATTE Martin
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2017007503A publication Critical patent/MX2017007503A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/2207—Sortase A (3.4.22.70)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

Abstract

En este documento se reporta de un método para producir un producto de conjugación enzimática de dos polipéptidos que comprende la incubación de un primer polipéptido que comprende la secuencia de aminoácidos LPXTG (SEC ID NO: 20, en donde X puede ser cualquier residuo de aminoácido), un segundo polipéptido tiene una secuencia de aminoácidos de oligo-alanina Am (m = 2 (SEC ID NO: 26) , o 3 (SEC ID NO: 27) o 4 (SEC ID NO: 28), o 5 (SEC ID NO: 29)) en su extremo N-terminal, un tercer polipéptido con actividad sortasa que se deriva de sortasa A de Staphylococcus aureus, y recuperar el conjugado de la mezcla de reacción y produciendo de este modo el producto de conjugación enzimática de dos polipéptidos.
MX2017007503A 2014-12-17 2015-12-14 Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa. MX2017007503A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14198532 2014-12-17
PCT/EP2015/079617 WO2016096741A1 (en) 2014-12-17 2015-12-14 Novel methods for enzyme mediated polypeptide conjugation using sortase

Publications (1)

Publication Number Publication Date
MX2017007503A true MX2017007503A (es) 2017-10-04

Family

ID=52338848

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017007503A MX2017007503A (es) 2014-12-17 2015-12-14 Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa.

Country Status (11)

Country Link
US (1) US10190182B2 (es)
EP (1) EP3233905B1 (es)
JP (1) JP6618539B2 (es)
KR (1) KR20170094216A (es)
CN (1) CN107001447B (es)
BR (1) BR112017010775A2 (es)
CA (1) CA2966287A1 (es)
MX (1) MX2017007503A (es)
RU (1) RU2714963C2 (es)
SG (1) SG11201704625UA (es)
WO (1) WO2016096741A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106191015B (zh) * 2016-08-25 2019-09-13 北京大学 金黄色葡萄球菌分选酶a高效突变体
WO2022166913A1 (en) * 2021-02-04 2022-08-11 Westlake Therapeutics (Hangzhou) Co. Limited Modified red blood cells and uses thereof for treating hyperuricemia and gout
WO2023134573A1 (en) * 2022-01-12 2023-07-20 Westlake Therapeutics (Shanghai) Co., Limited Modified cells and uses thereof
CN114487409B (zh) * 2022-04-14 2022-07-19 启德医药科技（苏州）有限公司 一种转肽酶活性的检测方法及检测试剂盒
CN116949004B (zh) * 2023-07-13 2024-04-09 苏州源启生物科技有限公司 一种转肽酶Sortase A及其制备方法和应用

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Also Published As

Publication number Publication date
WO2016096741A1 (en) 2016-06-23
US20170292166A1 (en) 2017-10-12
CN107001447A (zh) 2017-08-01
EP3233905B1 (en) 2020-01-29
RU2714963C2 (ru) 2020-02-21
JP6618539B2 (ja) 2019-12-11
BR112017010775A2 (pt) 2018-02-27
RU2017122029A3 (es) 2019-07-17
SG11201704625UA (en) 2017-07-28
JP2018500901A (ja) 2018-01-18
CA2966287A1 (en) 2016-06-23
CN107001447B (zh) 2021-07-23
EP3233905A1 (en) 2017-10-25
US10190182B2 (en) 2019-01-29
KR20170094216A (ko) 2017-08-17
RU2017122029A (ru) 2019-01-17

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