KR20230034767A - An use of bactericidal composition - Google Patents
An use of bactericidal composition Download PDFInfo
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- KR20230034767A KR20230034767A KR1020210117911A KR20210117911A KR20230034767A KR 20230034767 A KR20230034767 A KR 20230034767A KR 1020210117911 A KR1020210117911 A KR 1020210117911A KR 20210117911 A KR20210117911 A KR 20210117911A KR 20230034767 A KR20230034767 A KR 20230034767A
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- South Korea
- Prior art keywords
- topramezone
- azoxystrobin
- weight ratio
- downy mildew
- bactericidal composition
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 33
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 19
- IYMLUHWAJFXAQP-UHFFFAOYSA-N topramezone Chemical compound CC1=C(C(=O)C2=C(N(C)N=C2)O)C=CC(S(C)(=O)=O)=C1C1=NOCC1 IYMLUHWAJFXAQP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000005730 Azoxystrobin Substances 0.000 claims abstract description 24
- WFDXOXNFNRHQEC-GHRIWEEISA-N azoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 WFDXOXNFNRHQEC-GHRIWEEISA-N 0.000 claims abstract description 24
- 241000233679 Peronosporaceae Species 0.000 claims abstract description 19
- 235000009754 Vitis X bourquina Nutrition 0.000 claims abstract description 18
- 235000012333 Vitis X labruscana Nutrition 0.000 claims abstract description 18
- 235000014787 Vitis vinifera Nutrition 0.000 claims abstract description 18
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- 241000219095 Vitis Species 0.000 claims description 17
- 230000000855 fungicidal effect Effects 0.000 claims description 13
- 239000000417 fungicide Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- 230000002265 prevention Effects 0.000 claims description 5
- 230000002421 anti-septic effect Effects 0.000 claims description 4
- 239000002671 adjuvant Substances 0.000 claims description 2
- 239000002917 insecticide Substances 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 11
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- 240000006365 Vitis vinifera Species 0.000 abstract 1
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- 201000010099 disease Diseases 0.000 description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 21
- 238000002360 preparation method Methods 0.000 description 20
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- 229940079593 drug Drugs 0.000 description 15
- 230000000694 effects Effects 0.000 description 13
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- 235000002595 Solanum tuberosum Nutrition 0.000 description 8
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- 238000004945 emulsification Methods 0.000 description 5
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
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- 206010059866 Drug resistance Diseases 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000219094 Vitaceae Species 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
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- 239000003595 mist Substances 0.000 description 3
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- 238000012360 testing method Methods 0.000 description 3
- 231100000820 toxicity test Toxicity 0.000 description 3
- 239000012905 visible particle Substances 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 241000233622 Phytophthora infestans Species 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 2
- 230000003042 antagnostic effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229940000425 combination drug Drugs 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000006184 cosolvent Substances 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000001804 emulsifying effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 244000000177 oomycete pathogen Species 0.000 description 2
- 235000012015 potatoes Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 229940126062 Compound A Drugs 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 241000218936 Pseudomonas corrugata Species 0.000 description 1
- 239000005869 Pyraclostrobin Substances 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000003022 colostrum Anatomy 0.000 description 1
- 235000021277 colostrum Nutrition 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002888 effect on disease Effects 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 230000028644 hyphal growth Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- BSDQITJYKQHXQR-UHFFFAOYSA-N methyl prop-2-eneperoxoate Chemical compound COOC(=O)C=C BSDQITJYKQHXQR-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010428 oil painting Methods 0.000 description 1
- 230000010494 opalescence Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- GYICYQJEVCIYJY-UHFFFAOYSA-N thiophen-1-ylidenemethanone Chemical compound O=C=S1C=CC=C1 GYICYQJEVCIYJY-UHFFFAOYSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Abstract
Description
본 발명은 농업용 살균 조성물, 특히 토프라메존 및 아족시스트로빈을 유효성분으로 포함하는 살균 조성물 및 그의 용도에 관한 것이다. 본 발명은 추가로 농작물 질병의 예방 및 방제에서의 살균 조성물의 용도, 특히 포도 노균병(grape downy mildew) 및 감자 역병(potato late light)에서의 용도에 관한 것으로, 살충제 분야에 속한다.The present invention relates to an agricultural bactericidal composition, particularly a bactericidal composition comprising topramezone and azoxystrobin as active ingredients, and uses thereof. The present invention further relates to the use of the fungicidal composition in the prevention and control of crop diseases, in particular against grape downy mildew and potato late light, and belongs to the field of pesticides.
토프라메존(Topramezone)은 독특한 표적 부위와 새로운 작용 메커니즘을 가지고 있다. 속효성과 지속효과가 좋으며 빗물에 잘 견딘다. 매우 낮은 복용량에서도 좋은 방제 효과를 나타낼 수 있다. 난균류 병원체에 의한 식물병에 매우 효과적이다. 예를 들어 감자, 포도, 채소 및 기타 특산작물에 대한 난균류 병원체 질병 예를 들어 포도, 멜론, 양배추의 노균병(downy mildew), 토마토 및 감자 역병(late blight), 후추 마름병(pepper blight) 및 기타 질병에 우수한 방제 효과가 있다. 그러나 토프라메존은 단일 작용부위를 갖고 있어 중~고 수준의 내성 위험이 있어 내성 관리가 필요하다. 현재, 약물 내성의 출현을 효과적으로 지연시키고 약물의 수명 주기를 연장하기 위해 명 연구자들은 작용 기전이 다른 살진균제(fungicide)와 토프라메존을 결합하거나 순환적으로 사용한다. 예를 들어, 토프라메존은, 티아벤다졸(thiabensazole). 티오펜케톤(thiophene ketone) 등 뿐만 아니라 아족시스트로빈(azoxystrobin), 피라클로스트로빈(pyraclostrobin) 같은 메톡시아크릴레이트(methoxyacrylate)계 살진균제와 함께 사용되고 있으나, 병용효과는 크지 않다.Topramezone has a unique target site and novel mechanism of action. It has a fast and lasting effect, and is resistant to rainwater. Even at very low doses, it can show a good control effect. It is very effective against plant diseases caused by oomycete pathogens. Oomycete pathogen diseases on e.g. potatoes, grapes, vegetables and other specialty crops e.g. downy mildew on grapes, melons, cabbage, late blight on tomatoes and potatoes, pepper blight and others It has an excellent control effect on diseases. However, topramezone has a single site of action, so there is a risk of moderate to high resistance, so tolerance management is required. Currently, to effectively delay the emergence of drug resistance and extend the drug's life cycle, researchers are combining or cyclically using topramezone with fungicides with different mechanisms of action. For example, topramezone, thiabensazole. It is used together with methoxyacrylate-based fungicides such as azoxystrobin and pyraclostrobin as well as thiophene ketone, but the combined effect is not great.
아족시스트로빈은 Fusarium으로 인한 질병에 효과적이며 보호 및 치료 효과가 있다. 그것은 뿌리를 통해 흡수되고 잎에 위쪽으로 전달되고 잎의 아래쪽과 잎 사이로의 전도도가 낮다. 아족시스트로빈은 토마토, 포도 및 기타 작물의 줄기 기저부 썩음, 뿌리 썩음 및 속 괴사를 효과적으로 제어할 수 있다. 그러나 포도 노균병 및 감자 역병에 대한 방제 효과에 대한 선행 기술에 대한 연구는 거의 없다.Azoxystrobin is effective against diseases caused by Fusarium and has protective and therapeutic effects. It is absorbed through the roots and transmitted upwards to the leaves, with low conductance between the undersides of the leaves and between the leaves. Azoxystrobin can effectively control stem base rot, root rot and pith necrosis of tomatoes, grapes and other crops. However, there is little prior art study on the control effect against grape downy mildew and late blight of potatoes.
놀랍게도, 본 출원인은 단일 작용 부위를 갖고 약물 내성이 생기기 쉬운 토프라메존과 아족시스트로빈의 특정 비율의 조합이 약물 내성을 피하면서 포도 노균병 및 감자 역병을 예방하는 좋은 상승 효과를 얻을 수 있음을 발견했다. 그것은 살충제 품종의 생산 및 적용에 중요한 적용 가치가 있다. 지금까지 위의 두 가지 유효성분의 조합에 대한 보고는 없었다.Surprisingly, the present applicants have found that a combination of a specific ratio of topramezone and azoxystrobin, which has a single site of action and is prone to drug resistance, can achieve a good synergistic effect in preventing grape downy mildew and potato late blight while avoiding drug resistance. did. It has important application value in the production and application of insecticide varieties. So far, there has been no report on the combination of the above two active ingredients.
본 발명의 목적은 상승 효과가 좋고 살균제 내성의 발생을 효과적으로 지연시킬 수 있는 살균제 조성물을 제공하는 것이다.It is an object of the present invention to provide a fungicide composition that has a good synergistic effect and can effectively delay the development of fungicide resistance.
본 발명은 다음과 같은 기술적 해결책을 통해 상기 목적을 달성한다:The present invention achieves the above object through the following technical solutions:
살균 조성물의 유효성분은 토프라메존(topramezone)과 아족시스트로빈(Azoxystrobin)이고, 이들의 중량비는 5:1 내지 1:1인 것을 특징으로 하는 살균 조성물.The active ingredients of the bactericidal composition are topramezone and azoxystrobin, and their weight ratio is 5: 1 to 1: 1.
바람직하게는, 토프라메존과 아족시스트로빈의 중량비는 5:1-3:1이며, 보다 바람직하게는 4:1이다.Preferably, the weight ratio between topramezone and azoxystrobin is 5:1-3:1, more preferably 4:1.
또한, 본 발명은 상기 살균 조성물을 2-90 중량%를 포함하고, 나머지는 살충제-허용 담체 및 보조제인 살균제를 추가로 제공한다.In addition, the present invention further provides a disinfectant comprising 2-90% by weight of the disinfectant composition and the remainder being an insecticide-acceptable carrier and adjuvant.
바람직하게는, 상기 살균제는 상기 살균 조성물의 6-70 중량%를 구성하고, 보다 바람직하게는, 10-50 중량%를 구성한다.Preferably, the bactericidal agent constitutes 6-70% by weight of the bactericidal composition, more preferably, 10-50% by weight.
당업자에게 잘 알려진 방법에 따르면, 본 발명의 살균제는 농업에서 허용되는 임의의 투여 형태일 수 있다. 바람직한 투여 형태는 유화성 농축물(emulsifiable concentrate), 수분산성 과립(water dispersible granule), 습윤성 분말(wettable powder), 현탁액(suspension), 마이크로에멀젼 또는 물 에멀젼이다.According to methods well known to those skilled in the art, the fungicide of the present invention may be in any dosage form acceptable in agriculture. Preferred dosage forms are emulsifiable concentrates, water dispersible granules, wettable powders, suspensions, microemulsions or water emulsions.
또한, 본 발명은 각 성분의 함량이 하기와 같이 중량백분율(weight percentage)로 계산되는 것을 특징으로 하는 살균 유화성 농축물을 제공한다:In addition, the present invention provides a sterile emulsifiable concentrate characterized in that the content of each component is calculated in weight percentage as follows:
살균 성분 6-15%;Bactericidal component 6-15%;
복합 유화제 15%;complex emulsifier 15%;
공용매: 15%;co-solvent: 15%;
잔량의 용매.residual amount of solvent.
복합 유화제(composite emulsifier)는 Nongru 600#과 Ningru 1600#으로 구성되어 있으며 중량비는 2-5:1이다.The composite emulsifier is composed of Nongru 600# and Ningru 1600#, and the weight ratio is 2-5:1.
공용매(co-solvent)는 에탄올, 에틸 아세테이트, 이소프로판올, 이소부탄올 및 n-부탄올 중에서 선택되며, 용매는 용제 오일 No.150이다.The co-solvent is selected from ethanol, ethyl acetate, isopropanol, isobutanol and n-butanol, and the solvent is solvent oil No. 150.
바람직하게는, 2종의 복합 유화제의 중량비는 4:1이다.Preferably, the weight ratio of the two complex emulsifiers is 4:1.
본 발명의 살균 조성물은 완성된 제제, 즉 조성물 내의 물질이 혼합된 형태로 제공될 수 있고; 그것은 또한 사용하기 전에 배럴(탱크)에서 직접 혼합되는 단일 용량 제제로 제공될 수 있다. 본 발명의 농축물은 일반적으로 원하는 활성 물질 농도를 얻기 위해 물과 혼합된다.The germicidal composition of the present invention may be provided in a finished formulation, i.e., a mixture of the materials in the composition; It may also be presented as a single dose formulation that is mixed directly in the barrel (tank) prior to use. Concentrates of the present invention are generally mixed with water to obtain the desired active substance concentration.
본 발명은 또한 작물 및/또는 과일이 병에 걸리기 전 또는 후에, 본 발명의 살균 조성물 또는 살균제를 작물 및/또는 과일 및 이들이 재배 또는 저장되는 장소에 적용하는 살균 방법을 제공한다.The present invention also provides a method for sterilization in which the bactericidal composition or bactericide of the present invention is applied to crops and/or fruits and the place where they are grown or stored, before or after the crops and/or fruits are diseased.
또한, 본 발명은 작물 질병의 예방 및 방제를 위한 상기 살균 조성물 또는 살균제의 용도를 제공한다. 특히 포도 노균병과 감자 역병을 방제하는 데 사용된다.In addition, the present invention provides the use of the fungicide composition or fungicide for the prevention and control of crop diseases. In particular, it is used to control grape downy mildew and potato blight.
종래 기술과 비교하여, 본 발명은 다음과 같은 유익한 효과를 제공한다:Compared with the prior art, the present invention provides the following beneficial effects:
(1) 단일 제제와 비교하여, 본 발명의 살균 조성물은 질병에 대한 유의한 상승 효과를 갖고 살균 효과를 개선하며 135 이상의 공독성 계수(co-toxicity coefficient)를 갖는다.(1) Compared with a single agent, the bactericidal composition of the present invention has a significant synergistic effect on disease, improves bactericidal effect, and has a co-toxicity coefficient of 135 or more.
(2) 본 발명의 조성물은 작용 기전이 다른 유효 성분으로 구성되어 있으며 작용 부위의 증가는 세균 내성의 발달을 극복하고 지연시키는 데 유익하다.(2) The composition of the present invention is composed of active ingredients with different mechanisms of action, and the increase in the action site is beneficial for overcoming and delaying the development of bacterial resistance.
(3) 또한, 본 발명의 살균 조성물은 유화성 농축물로 제조되어 특정 복합 유화제를 선택함으로써 분산성, 유화성 및 안정성 특성과 관련된 유화 효과를 크게 향상시킬 수 있다.(3) In addition, the bactericidal composition of the present invention is prepared from an emulsifiable concentrate, and the emulsification effect related to dispersibility, emulsification and stability characteristics can be greatly improved by selecting a specific complex emulsifier.
본 발명의 목적, 기술적 해결방안 및 이점을 보다 명확하게 하기 위하여, 이하의 구체적인 실시예를 사용하여 설명하지만, 본 발명이 이들 실시예에 한정되는 것은 아니다. 다음은 본 발명을 설명하기 위한 이러한 방식의 좋은 예일 뿐이며, 이는 본 발명의 범위를 제한하는 것으로 이해될 수 없다. 본 발명의 사상과 원리 내에서 이루어진 모든 수정, 균등 교체 또는 개선은 본 발명의 보호 범위에 포함되어야 한다.In order to make the objects, technical solutions and advantages of the present invention more clear, the following specific examples are used to describe the present invention, but the present invention is not limited to these examples. The following are only good examples of this manner of describing the present invention, which should not be construed as limiting the scope of the present invention. Any modification, equivalent replacement or improvement made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
I. 실내 활성 예(Indoor Activity Examples)I. Indoor Activity Examples
시험약(Test drug)Test drug
95% 토프라메존 오리지널 약물.95% topramezone original drug.
95% 아족시스트로빈.95% azoxystrobin.
1 시험 방법(Test method)1 Test method
1.1 실내 독성 시험(Indoor toxicity test)1.1 Indoor toxicity test
1.1.1 포도 노균병의 실내 독성 측정1.1.1 Measurement of indoor toxicity of grape downy mildew
(1) 포자낭 현탁액의 제조(preparation of sporangia suspension)(1) Preparation of sporangia suspension
신선한 포도 노균병 잎을 수집하고, 병든 잎에 있는 오래된 곰팡이 층(mildew layer)을 솔을 사용하여 흐르는 물에 씻어 내고, 잎을 바닥에 두 겹의 흡수성 거즈가 있는 보습 트레이에 다시 펴고, 보습 트레이는 플라스틱 필름으로 밀봉되었다. 그런 다음 배양 조건 18℃, 상대 습도 90%, 명암(명 12h, 암 12h)이 교차하는 인공 기후 인큐베이터에 넣었다. 병변에서 새로운 포자낭이 자라난 후 증류수로 헹구고 희석하여 1×105/L의 포자낭 현탁액을 제조하여 사용하였다.Collect fresh grape downy mildew leaves, wash the old mildew layer on the diseased leaves with running water using a brush, spread the leaves again on a moisturizing tray with two layers of absorbent gauze on the bottom, and the moisturizing tray is sealed with plastic film. Then, they were placed in an artificial climate incubator with alternating culture conditions of 18°C, 90% relative humidity, and light/dark conditions (12 h light, 12 h dark). After new sporangia grew in the lesion, it was rinsed with distilled water and diluted to prepare a 1×10 5 /L sporangium suspension.
(2) 실내 독성 시험(2) Indoor toxicity test
약제(medicament)를 접종 24시간 전에 처리하였다. 직경 1.5cm의 잎 디스크(disc) 뒷면에 다양한 처리액을 뿌렸다. 액을 자연풍 건조시킨 후 각 처리잎 디스크의 뒷면을 페트리 접시에 올려놓고 페트리 접시의 바닥을 같은 액체로 적신 흡수지로 덮었다. 15개의 잎 디스크를 각 접시에 동심원으로 배치하고 각 접시에 대해 4번 반복했다. 블랭크 대조군을 0.1% Tween-80 수용액으로 처리하였다. 24시간 처리 후 세균 접종. 피펫을 사용하여 포자낭 현탁액을 잎 디스크 중앙에 접종했다(잎 디스크당 20μL). 인공 기후 인큐베이터에서 배양 조건: 온도 22℃, 상대 습도 90%, 명암 교대(명 12h, 암 12h).Medicaments were administered 24 hours before inoculation. Various treatment solutions were sprayed on the back of leaf discs with a diameter of 1.5 cm. After drying the liquid in the air, the back side of each treated leaf disc was placed on a Petri dish, and the bottom of the Petri dish was covered with an absorbent paper soaked in the same liquid. Fifteen leaf discs were concentrically placed in each dish and repeated 4 times for each dish. A blank control was treated with a 0.1% Tween-80 aqueous solution. Bacterial inoculation after 24 hours of treatment. The sporangial suspension was inoculated into the center of the leaf disc using a pipette (20 μL per leaf disc). Incubation conditions in an artificial climatic incubator: temperature 22 °C, relative humidity 90%, light/dark alternating (light 12 h, dark 12 h).
(3) 조사방법(3) Survey method
대조군이 고르게 발달되었을 때 결과를 조사하였다. 잎 디스크의 면적에 대한 질병 반점(diseased spot)의 면적의 백분율에 따라 병의 중증도를 결정하고 병든 잎 디스크의 수와 병의 중증도를 기록하고 공식에 따라 질병 지수(disease index) 및 방제 효과(control effect)를 계산하였다.Results were examined when the control group developed evenly. Determine the severity of the disease according to the percentage of the area of the diseased spot to the area of the leaf disc, record the number of diseased leaf discs and the severity of the disease, and calculate the disease index and control effect (control) according to the formula effect) was calculated.
질병 등급 기준의 중증도: Severity of disease grading criteria:
등급 0(Grade 0): 질병 반점이 없음; Grade 0: no disease spots;
등급 1: 질병 반점의 면적이 전체 잎 면적의 5% 이하;Grade 1: disease spot area less than 5% of total leaf area;
등급 3: 질병 반점의 면적이 전체 잎 면적의 6%-25%;Grade 3: Area of disease spots 6%-25% of total leaf area;
등급 5: 질병 반점의 면적이 전체 잎 면적의 26%-50%;Grade 5: Area of disease spots 26%-50% of total leaf area;
등급 7: 질병 반점의 면적은 전체 잎 면적의 51%-75%;Grade 7: Area of disease spots 51%-75% of total leaf area;
등급 9: 질병 반점의 면적이 전체 잎 면적의 76% 이상.Grade 9: Area of disease spots greater than or equal to 76% of total leaf area.
질병 지수 = (∑ 균주수 * 대표값) / (총 균주수 * 최대 대표값) * 100Disease index = (∑ number of strains * representative value) / (total number of strains * maximum representative value) * 100
방제 효과 = 1 - (투여 후 치료군의 질병 지수 * 투여 전 대조군의 질병 지수) / (투여 전 치료군의 질병 지수 * 투여 후 대조군의 질병 지수) * 100%Control effect = 1 - (Disease index of treatment group after administration * Disease index of control group before administration) / (Disease index of treatment group before administration * Disease index of control group after administration) * 100%
1.1.2 감자 역병의 실내 독성 측정1.1.2 Measurement of indoor toxicity of potato blight
균사체 증식 억제 방법을 채용하였다: 약용액 0.5mL와, 0.1% 스트렙토마이신 설페이트(streptomycin sulfate)를 함유하는 녹인 PDA 배지 4.5mL를 취하여 멸균된 페트리 접시(직경 5cm)에 부어 약으로 배지 플레이트를 만들었다. 배지가 고형화된 후 각 배지면에 감자 감염 세균 케이크(직경 0.5cm) 1개를 놓고 균사가 있는 균류 측을 배지 표면에 붙였다. 각 처리는 3개의 페트리 접시에 대해 반복되었고, 무균 수용액을 대조군으로 사용하였다. 25℃에서 72시간 동안 배양한 후, 대조군의 균사체 성장에 따라 다른 농도의 화합물 A 처리 후 균사체의 성장을 기록하였다. 십자법을 이용하여 집락 직경(colony diameter)(mm)을 자로 2회 측정하여 평균값을 산출하였다. 균사체 성장 억제율(mycelial growth inhibition rate)은 다음 식에 따라 계산하였다.A method for inhibiting mycelial growth was employed: 0.5 mL of the drug solution and 4.5 mL of melted PDA medium containing 0.1% streptomycin sulfate were taken and poured into a sterilized Petri dish (5 cm in diameter) to make a medium plate with the drug. After the medium was solidified, one potato-infected bacterial cake (0.5 cm in diameter) was placed on each medium side, and the fungus side with hyphae was attached to the medium surface. Each treatment was repeated for 3 Petri dishes, and a sterile aqueous solution was used as a control. After incubation at 25 ° C. for 72 hours, the mycelial growth was recorded after treatment with Compound A at different concentrations according to the mycelial growth of the control group. The average value was calculated by measuring the colony diameter (mm) twice with a ruler using the cross method. Mycelial growth inhibition rate (mycelial growth inhibition rate) was calculated according to the following formula.
억제율 = {(대조군 균사의 순생장 - 처리된 균사의 순생장) / 처리된 균사의 순생장} * 100%Inhibition rate = {(net growth of control hyphae - net growth of treated hyphae) / net growth of treated hyphae} * 100%
참고: 균사의 순 성장 = 집락 직경 - 펀치 직경Note: net hyphal growth = colony diameter - punch diameter
1.1.3 데이터 처리 및 분석1.1.3 Data processing and analysis
독립변수 x로서 약제 농도의 로그를, 회귀분석을 위한 종속변수 y로서 방제 효과 또는 억제율의 확률을 취하였으며, 독성 회귀곡선식(regression equation) y=a+bx 및 상관계수(correlation coefficient) r을 얻었고, 토프라메존과 아족시스트로빈의 단일 용량의 EC50 및 95% 신뢰 구간(confidence interval)은 회귀 방정식에 따라 계산되었다.The logarithm of the drug concentration was taken as the independent variable x, and the probability of control effect or inhibition rate was taken as the dependent variable y for regression analysis, and the toxicity regression equation y = a + bx and the correlation coefficient r were obtained , EC 50 and 95% confidence intervals of single doses of topramezone and azoxystrobin were calculated according to the regression equation.
1.2 살진균제 화합물의 복합 독성 측정1.2 Determination of complex toxicity of fungicidal compounds
토프라메존과 아족시스트로빈 단일 용량의 독성 시험 결과에 따라, 복합제는 7:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2의 부피비에 따라 제형화되었다. 감자 역병 및 포도 노균병에 대한 화합물의 항균 효과는 방법 1.1에 따라 측정하였고, 공독성 계수(CTC:co-toxicity coefficient)는 Sun Yunpei(1960) 방법으로 계산하였다. CTC≥120이면 상승 효과(synergic effect)를 의미하고; CTC≤80은 길항 효과(antagonistic effect)를 의미하고; 80<CTC<120은 부가 효과(additive effect)를 의미한다.According to the toxicity test results of single doses of topramezone and azoxystrobin, the combination formulation was formulated according to the volume ratio of 7:1, 5:1, 4:1, 3:1, 2:1, 1:1, and 1:2. got angry The antibacterial effect of the compound against potato blight and grape downy mildew was measured according to method 1.1, and the co-toxicity coefficient (CTC) was calculated according to the method of Sun Yunpei (1960). CTC≧120 indicates a synergic effect; CTC≤80 means antagonistic effect; 80<CTC<120 means an additive effect.
독성 지수(TI) = (표준 약제의 EC50 / 시험 약제의 EC50) * 100%Toxicity index (TI) = (EC 50 of standard agent / EC 50 of test agent) * 100%
혼합 약제(M)의 실제 독성 지수(ATI) = (표준 약제의 EC50 / 혼합 약제 M의 EC50) * 100Actual toxicity index (ATI) of combination drug (M) = (EC 50 of standard drug / EC 50 of combination drug M ) * 100
혼합 약제 M의 이론적 독성 지수(TTI) = 약물 A의 TI * 약물 M 중의 약물 A의 % + 약물 B의 TI * 약물 M 중의 약물 B의 %Theoretical Toxicity Index (TTI) of Mixed Drug M = TI of Drug A * % of Drug A in Drug M + TI of Drug B * % of Drug B in Drug M
혼합 약제 M의 이론적 독성 지수(TTI) = (ATI / TTI) * 100Theoretical toxicity index (TTI) of mixed drug M = (ATI / TTI) * 100
1.3 방제 효과1.3 Control effect
포도 노균병 및 감자 역병의 방제 효과는 표 1 및 표 2에 나와 있다.The control effects of grape downy mildew and potato blight are shown in Tables 1 and 2.
표 1: 포도 노균병 방제 효과Table 1: Grape Downy Mildew Control Effect
상기 표 1로부터 토프라메존:아족시스트로빈의 중량비가 5:1 내지 1:1인 경우, 특히 이들의 중량비가 4:1인 경우 상승 효과가 현저함을 알 수 있다. 토프라메존:아족시스트로빈의 중량비가 5:1 ~ 1:1의 범위에 있지 않은 경우, 예를 들어 중량비가 7:1 또는 1:2인 경우, 토프라메존:아족시스트로빈의 조합은 단지 부가 효과를 나타낸다.From Table 1, it can be seen that when the weight ratio of topramezone:azoxystrobin is 5:1 to 1:1, in particular, when the weight ratio is 4:1, the synergistic effect is remarkable. When the weight ratio of topramezone:azoxystrobin is not in the range of 5:1 to 1:1, for example, when the weight ratio is 7:1 or 1:2, the combination of topramezone:azoxystrobin is only represents an additive effect.
표 2: 감자 역병 방제 효과Table 2: Potato blight control effect
상기 표 2로부터 토프라메존:아족시스트로빈의 중량비가 5:1 내지 1:1인 경우, 특히 이들의 중량비가 4:1인 경우 이들의 상승 효과가 현저함을 알 수 있다. 토프라메존:아족시스트로빈의 중량비가 5:1 내지 1:1의 범위에 있지 않은 경우, 예를 들어 중량비가 7:1인 경우, 토프라메존:아족시스트로빈의 조합은 부가 효과만을 나타낸다. 토프라메존:아족시스트로빈의 중량비가 1:2일 때, 그 조합은 길항 효과를 나타낸다.From Table 2, it can be seen that when the weight ratio of topramezone:azoxystrobin is 5:1 to 1:1, especially when the weight ratio is 4:1, their synergistic effect is remarkable. When the weight ratio of topramezone:azoxystrobin is not in the range of 5:1 to 1:1, for example, when the weight ratio is 7:1, the combination of topramezone:azoxystrobin shows only an additive effect. When the weight ratio of topramezone:azoxystrobin is 1:2, the combination exhibits an antagonistic effect.
2. 제조예(Preparation Examples)2. Preparation Examples
제조예 1:Preparation Example 1:
200ml 플라스크에 용매 오일 No.150(공정 180-210℃, 유출 ≥98%, 인화점 ≥62℃) 60g을 첨가한 후, 이소프로판올(분석 등급) 15g을 첨가하고, 유리 막대로 5분 동안 교반하였다. 상기 혼합용액에 살균 조성물(토프라메존과 아족시스트로빈의 중량비는 2:1) 10g을 첨가하고 유리 막대로 10분간 교반하였다. 그 결과 혼합액에 Nongru 600#과 Ningru 1600#을 혼합(중량비는 2:1)하여 만든 유화제 15g을 첨가하였다. 5분 동안 교반한 후, 10% 토프라메존 및 아족시스트로빈 EC 제형을 제조하였다.After adding 60g of solvent oil No. 150 (process 180-210°C, efflux ≥98%, flash point ≥62°C) to a 200ml flask, 15g of isopropanol (analytical grade) was added and stirred for 5 minutes with a glass rod. 10 g of the antiseptic composition (weight ratio of topramezone and azoxystrobin: 2:1) was added to the mixed solution and stirred for 10 minutes with a glass rod. As a result, 15 g of an emulsifier made by mixing Nongru 600# and Ningru 1600# (weight ratio 2:1) was added to the mixed solution. After stirring for 5 minutes, a 10% topramezone and azoxystrobin EC formulation was prepared.
제조예 2:Preparation Example 2:
Nongru 600#과 Ningru 1600#의 중량비가 5:1인 유화제 15g을 첨가한 것을 제외하고는 제조예 1과 동일하다.It is the same as in Preparation Example 1 except for adding 15 g of an emulsifier having a weight ratio of Nongru 600# and Ningru 1600# of 5:1.
제조예 3:Preparation Example 3:
Nongru 600#과 Ningru 1600#의 중량비가 4:1인 유화제 15g을 첨가한 것을 제외하고는 제조예 1과 동일하다.It is the same as in Preparation Example 1 except for adding 15 g of an emulsifier having a weight ratio of Nongru 600# and Ningru 1600# of 4:1.
제조예 4:Preparation Example 4:
Nongru 600#과 Ningru 1600#의 중량비가 1:1인 유화제 15g을 첨가한 것을 제외하고는 제조예 1과 동일하다.It is the same as in Preparation Example 1 except that 15 g of an emulsifier having a weight ratio of Nongru 600# and Ningru 1600# is 1:1 was added.
제조예 5:Preparation Example 5:
Nongru 600#과 Ningru 1600#의 중량비가 6:1인 유화제 15g을 첨가한 것을 제외하고는 제조예 1과 동일하다.It is the same as in Preparation Example 1 except for adding 15 g of an emulsifier having a weight ratio of Nongru 600# and Ningru 1600# of 6:1.
비교예 1:Comparative Example 1:
유화제로 Ningru 600# 15g을 첨가한 것을 제외하고는 제조예 1과 동일하다.It is the same as in Preparation Example 1 except that 15 g of Ningru 600# was added as an emulsifier.
비교예 2:Comparative Example 2:
유화제로 Ningru 1600# 15g을 첨가한 것을 제외하고는 제조예 1과 동일하다.It is the same as in Preparation Example 1 except that 15 g of Ningru 1600 # was added as an emulsifier.
비교예 3:Comparative Example 3:
유화제로 Ningru 500# 15g을 첨가한 것을 제외하고는 제조예 1과 동일하다.It is the same as in Preparation Example 1 except that 15 g of Ningru 500# was added as an emulsifier.
비교예 4:Comparative Example 4:
유화제로 EL-40 15g을 첨가한 것을 제외하고는 제조예 1과 동일하다.It is the same as in Preparation Example 1 except that 15 g of EL-40 was added as an emulsifier.
3. 유화 효과 시험의 예3. Example of emulsifying effect test
1. 에멀젼 안정성의 측정 방법은 GB/T1603-2001 방법에 따랐다.1. The emulsion stability measurement method was in accordance with the GB/T1603-2001 method.
표준 경수(standard hard water)의 조제(경도는 탄산칼슘 기준으로 342mg·L-1임)Preparation of standard hard water (hardness is 342 mg L-1 based on calcium carbonate)
무수 염화칼슘 0.304g(정확도 0.0002g, 이하 동일)과 염화마그네슘 0.139g을 수정수(crystal water)와 함께 1000mL 비커에 넣고 증류수 600mL를 넣어 녹여, 1000mL 메스플라스크(volumetric flask)에 넣는다. 비이커를 증류수 300mL로 헹구고 헹굼액을 메스플라스크에 붓고, 증류수로 표시선까지 채우고 잘 흔든 다음 따로 둔다.Put 0.304 g of anhydrous calcium chloride (accuracy 0.0002 g, hereinafter the same) and 0.139 g of magnesium chloride together with crystal water in a 1000 mL beaker, add 600 mL of distilled water to dissolve, and pour into a 1000 mL volumetric flask. Rinse the beaker with 300 mL of distilled water, pour the rinse into a volumetric flask, fill it up to the mark with distilled water, shake well, and set aside.
250mL 비이커에 25~30℃ 표준 경수 100mL를 넣고 상기 제조예 및 비교예의 유화성 농축물(20배 희석) 0.5mL를 취하여 일정하게 교반하면서 천천히 경수에 첨가하였다. 유화성 농축물 시료를 첨가한 후, 30초 동안 2-3rps의 속도로 계속 교반한 후 즉시 에멀젼을 깨끗하고 건조한 100mL 눈금 실린더에 옮기고 눈금 실린더를 (30±2)℃ 항온 수조(water bath)에서 1시간 동안 두고 에멀젼의 분리를 관찰하였다. 눈금 실린더에 슬릭오일(slick oil)(페이스트), 오일 침강(oil sinking) 또는 침전이 없으면, 유효 안정성은 적격으로 간주한다.Into a 250mL beaker, 100mL of standard hard water at 25-30 ° C was taken, and 0.5mL of the emulsifiable concentrate (20-fold dilution) of the above Preparation Examples and Comparative Examples was slowly added to the hard water with constant stirring. After adding the emulsifiable concentrate sample, continue stirring at a rate of 2-3 rps for 30 seconds, and then immediately transfer the emulsion to a clean, dry 100 mL graduated cylinder and incubate the graduated cylinder in a (30 ± 2) ° C constant temperature water bath. It was left for 1 hour and the separation of the emulsion was observed. Effective stability is considered acceptable if there is no slick oil (paste), oil sinking or precipitation on the graduated cylinder.
2. 유화 및 분산의 결정 방법 (Guo Wudi, 2003)2. Determination method of emulsification and dispersion (Guo Wudi, 2003)
100mL 눈금 실린더에 증류수 99.5mL를 넣고 상기 제조예 및 비교예의 유화성 농축물 0.5mL를 옮겨 분산 상태를 관찰하였다. 눈금 실린더에 유화성 농축물을 떨어뜨린 후 마개를 덮고 눈금 실린더를 15회 돌려 초유 상태(colostrum state)를 관찰하였다. 유화 및 분산성에 대한 평가 기준은 다음과 같다.99.5 mL of distilled water was added to a 100 mL graduated cylinder, and 0.5 mL of the emulsifiable concentrates of the above Preparation Examples and Comparative Examples were transferred and the dispersion state was observed. After dropping the emulsifiable concentrate on the graduated cylinder, the stopper was covered and the graduated cylinder was rotated 15 times to observe the colostrum state. Evaluation criteria for emulsification and dispersibility are as follows.
분산 평가 기준:Variance evaluation criteria:
우수(Excellent): 기본적으로 눈에 보이는 입자 없이, 자동으로 파란색 형광 유백색 미스트(milky white mist)로 분산되고 자동으로 위쪽으로 뒤집힐 수 있으며, 벽에 파란색 유제(emulsion) 필름이 있다.Excellent: basically no visible particles, automatically dispersed as a blue fluorescent milky white mist and can be automatically flipped upwards, with a blue emulsion film on the wall.
좋음(Good): 대부분의 유화성 농축물이 눈에 보이는 입자가 몇 개 또는 소량의 슬릭 오일이 있는 유백색 미스트로 자동으로 분산된다.Good: Most emulsifiable concentrates automatically disperse as a milky mist with a few visible particles or a small amount of slick oil.
예(Yes): 유백색 미스트로 분산될 수 있다.Yes: Can be dispersed as a milky white mist.
나쁨(Poor): 분산 없음, 기름 방울 또는 입자 가라앉음.Poor: No dispersion, oil droplets or particles settling.
유화 평가 기준:Oil painting evaluation criteria:
우수(Excellent): 에절전(emulsion)은 청색 투명 또는 반투명이며 유백색(opalescence)이 강하다.Excellent: The emulsion is blue transparent or translucent, with strong opalescence.
좋음(Good): 에멀전은 진한 유백색 또는 약간 푸른빛을 띠며 바닥에 유백색이 있고 유제 벽에 필름이 있다.Good: The emulsion is dark milky white or slightly bluish, with a milky white color at the bottom and a film on the emulsion wall.
예(Yes): 에멀전이 유화된 상태이며 광택이 없다.Yes: The emulsion is emulsified and has no luster.
나쁨(Poor): 에멀전이 눈에 보이는 입자가 있는 회백색이다.Poor: The emulsion is off-white with visible particles.
3. 실험 결과3. Experimental results
실험 결과를 하기 표 3에 나타내었다:The experimental results are shown in Table 3 below:
표 3 다양한 유화제의 유화 효과Table 3 Emulsifying effect of various emulsifiers
위의 표를 보면 Nongru 600#과 Ningru 1600#으로 구성된 복합 유화제를 선택했을 때, 즉 제조예 1-5가 단일 제제를 선택한 다른 유화제나 비교예보다 우수한 유화 효과를 가짐을 알 수 있다. 복합 유화제 자체는 둘의 중량비가 2-5:1일 때 다른 비율보다 우수하며, 특히 둘의 중량비가 4:1일 때, 즉 제조예 3이 효과가 가장 우수하다. Looking at the table above, it can be seen that when a composite emulsifier composed of Nongru 600# and Ningru 1600# is selected, that is, Preparation Examples 1-5 have a superior emulsification effect than other emulsifiers or comparative examples in which a single agent is selected. The composite emulsifier itself is superior to other ratios when the weight ratio of the two is 2-5:1, and in particular, when the weight ratio of the two is 4:1, that is, Preparation Example 3 is the most effective.
토프라메존 및 아족시스트로빈을 함유하는 본 발명의 살균 조성물을 구체적인 실시예로 설명하였다. 당업자는 본 발명의 내용으로부터 학습하고 상응하는 다른 목적을 달성하기 위해 원료, 공정 조건 및 기타 링크를 적절하게 변경할 수 있다. 관련된 변경 사항은 본 발명의 내용에서 벗어나지 않는다. 이와 유사한 대체 및 수정은 모두 당업자에게 자명하므로 모두 본 발명의 범위에 포함되는 것으로 간주한다.The antiseptic composition of the present invention containing topramezone and azoxystrobin has been described in specific examples. A person skilled in the art can appropriately change raw materials, process conditions and other links to learn from the content of the present invention and achieve other corresponding purposes. Relevant changes do not depart from the content of the present invention. Similar substitutions and modifications are all obvious to those skilled in the art and are therefore considered to be within the scope of the present invention.
Claims (6)
포도 노균병 예방 및 방제용 살균 조성물.A bactericidal composition for preventing and controlling grape downy mildew, the bactericidal composition comprising topramezone and azoxystrobin as active ingredients in a weight ratio of 5: 1 to 1: 1,
Bactericidal composition for preventing and controlling grape downy mildew.
토프라메존 및 아족시스트로빈의 중량비가 5:1 내지 3:1인,
포도 노균병 예방 및 방제용 살균 조성물.According to claim 1,
The weight ratio of topramezone and azoxystrobin is 5:1 to 3:1,
Bactericidal composition for preventing and controlling grape downy mildew.
토프라메존 및 아족시스트로빈의 중량비가 4:1인,
포도 노균병 예방 및 방제용 살균 조성물.According to claim 1,
The weight ratio of topramezone and azoxystrobin is 4:1,
Bactericidal composition for preventing and controlling grape downy mildew.
포도 노균병 예방 및 방제용 살균제.A fungicide for preventing and controlling grape downy mildew, comprising the fungicide composition of claim 1 and an acceptable carrier and adjuvant for an insecticide, wherein the weight of the fungicide composition is 2 to 90%,
Fungicide for the prevention and control of grape downy mildew.
포도 노균병 예방 및 방제용 살균제.The method of claim 4, wherein the weight of the antiseptic composition is 6-70%,
Fungicide for the prevention and control of grape downy mildew.
포도 노균병 예방 및 방제용 살균제.
The method of claim 4, wherein the weight of the antiseptic composition is 6-70%,
Fungicide for the prevention and control of grape downy mildew.
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