KR20110046537A - 콜라겐매개된 질병을 치료하기 위한 조성물 및 방법 - Google Patents

콜라겐매개된 질병을 치료하기 위한 조성물 및 방법 Download PDF

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Publication number: KR20110046537A
Authority: KR; South Korea
Prior art keywords: collagenase; drug; fermentation; lane; sample
Prior art date: 2006-01-30

Application number

KR1020117006197A

Other languages

English (en)

Korean (ko)

Inventor

그레고리 엘. 사바티노

벤자민 제이. 주니어 델 티토

필립 제이. 바셋

헤이즐 에이. 타리아

안토니 지. 히치콕

Original Assignee

옥실리엄 인터내셔널 홀딩스, 인코포레이티드

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2006-01-30

Filing date

2007-01-30

Publication date

2011-05-04

2007-01-30 Application filed by 옥실리엄 인터내셔널 홀딩스, 인코포레이티드 filed Critical 옥실리엄 인터내셔널 홀딩스, 인코포레이티드

2011-05-04 Publication of KR20110046537A publication Critical patent/KR20110046537A/ko

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- C12N9/14—Hydrolases (3)
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- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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US11/699,302 US7811560B2 (en)	2006-01-30	2007-01-29	Compositions and methods for treating collagen-mediated diseases
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KR1020087021302A KR20080093142A (ko)	2006-01-30	2007-01-30	콜라겐매개된 질병을 치료하기 위한 조성물 및 방법

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US11879141B2 (en)	2012-01-12	2024-01-23	Endo Global Ventures	Nucleic acid molecules encoding clostridium histolyticum collagenase II and methods of producing the same

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6958150B2 (en) *	1994-12-15	2005-10-25	Advance Biofactures Of Curacao, N.V.	Reduction of adipose tissue
US20070224184A1 (en) *	2006-02-22	2007-09-27	The Research Foundation Of The State University Of New York	Method for treating cellulite
US7811560B2 (en) *	2006-01-30	2010-10-12	Auxilium Us Holdings, Llc	Compositions and methods for treating collagen-mediated diseases
US10071143B1 (en)	2007-05-03	2018-09-11	The Research Foundation For The State University Of New York	Methods for non-surgical treatment of carpal tunnel syndrome
EP2244735A4 (en)	2007-10-02	2011-02-23	Avaxia Biologics Inc	ANTIBODY THERAPY FOR USE IN THE DIGESTIVE TUBE
US20100159564A1 (en) *	2007-11-30	2010-06-24	Dwulet Francis E	Protease resistant recombinant bacterial collagenases
TWI395593B (zh)	2008-03-06	2013-05-11	Halozyme Inc	可活化的基質降解酵素之活體內暫時性控制
EP2130551B1 (en)	2008-06-02	2012-06-06	Roche Diagnostics GmbH	Improved purification of collagenases from clostridium histolyticum liquid culture
US8236356B2 (en)	2008-06-11	2012-08-07	Roche Diagnostics Operations, Inc.	Growth medium for Clostridium histolyticum
WO2010102262A1 (en)	2009-03-06	2010-09-10	Halozyme, Inc.	Temperature sensitive mutants of matrix metalloprotease 1 und uses thereof
ES2385239B1 (es)	2010-09-30	2013-06-19	Proteos Biotech S.L.U.	Uso de colagenasa g recombinante, colagenasa h recombinante y pz-peptidasa recombinante para el tratamiento de enfermedades que cursan con alteraciones del colágeno.
US10119131B2 (en) *	2011-03-16	2018-11-06	Biospecifics Technologies Corp.	Compositions and methods for producing clostridial collagenases
PL2734216T3 (pl)	2011-07-20	2018-12-31	Mediwound, Ltd.	Ekstrakt proteolityczny z bromelainy do leczenia schorzeń tkanki łącznej
DK3581199T3 (da) *	2011-10-21	2023-09-04	Endo Global Ventures	Fremgangsmåder til behandling eller reduktion af ødematøs fibrosklerotisk pannikulitis
ITPD20120118A1 (it)	2012-04-18	2013-10-19	Fidia Farmaceutici	"nuovo processo di produzione e purificazione dell'enzima collagenasi da vibrio alginolyticus"
CA2872227A1 (en)	2012-05-01	2013-11-07	Proteolease Ltd.	Methods for extracting a tooth
BR102012013110A2 (pt)	2012-05-31	2014-05-27	Cristalia Prod Quimicos Farm	Meio de cultura para bactérias do gênero clostridium livre de componentes de origem animal e processo para produção de sobrenadante contendo uma ou mais proteases com atividade colagenolítica e gelatinolítica
WO2014066622A2 (en)	2012-10-24	2014-05-01	The Research Foundation For The State University Of New York	Use of collagenase to treat glaucoma
WO2014107745A1 (en)	2013-01-07	2014-07-10	Halozyme, Inc.	Metal sensitive mutants of matrix metalloproteases and uses thereof
US20160000890A1 (en)	2013-03-15	2016-01-07	Biospecifics Technologies Corp.	Thermosensitive hydrogel collagenase formulations
US10369110B2 (en) *	2013-03-15	2019-08-06	Biospecifics Technologies Corporation	Treatment method and product for uterine fibroids using purified collagenase
US9849133B2 (en) *	2013-06-18	2017-12-26	Eton Pharmaceuticals, Inc.	Pharmaceutical formulations of xanthine or xanthine derivatives, and their use
US10383875B2 (en)	2013-06-18	2019-08-20	Harrow Ip, Llc	Pharmaceutical formulations of xanthine or xanthine derivatives, and their use
US10117892B2 (en) *	2013-08-29	2018-11-06	Allergan, Inc.	Devices and methods for reducing the appearance of cellulite
CN103751102A (zh)	2014-01-15	2014-04-30	上海交通大学	一种胶原酶温敏水凝胶及其制备方法和应用
CN105412916B (zh) *	2014-09-19	2021-04-30	达森生物药业有限公司	治疗乳腺癌的组合物及其用途
CN105477628B (zh) *	2014-09-19	2021-04-30	山东蓝金生物工程有限公司	抗癌组合物及其用途
CN105412917B (zh) *	2014-09-19	2021-04-30	达森生物药业有限公司	治疗实体瘤的抗癌组合物及其用途
CN105477627B (zh) *	2014-09-19	2021-04-30	山东蓝金生物工程有限公司	治疗***癌的组合物及其用途
DE202014105440U1 (de) *	2014-11-12	2016-02-15	Bilz Werkzeugfabrik Gmbh & Co. Kg	Werkzeugaufnahme
KR101723168B1 (ko)	2015-04-28	2017-04-05	주식회사 대웅	보툴리눔 독소의 제조를 위한 배지 조성물
US10303245B2 (en) *	2015-05-04	2019-05-28	Adobe Inc.	Methods and devices for detecting and responding to changes in eye conditions during presentation of video on electronic devices
CN115969318A (zh)	2017-03-01	2023-04-18	恩多风投有限公司	用于评估和治疗橘皮组织的装置和方法
KR102642381B1 (ko) *	2017-03-28	2024-02-28	엔도 벤쳐즈 리미티드	개선된 콜라게나제 생성 방법
WO2020021330A2 (en) *	2018-07-12	2020-01-30	Endo Global Aesthetics Limited	Injection techniques for the treatment of cellulite
WO2020021332A2 (en)	2018-07-12	2020-01-30	Endo Global Aesthetics Limited	Injection techniques for the treatment of cellulite
CN113015514A (zh)	2018-09-18	2021-06-22	恩多全球美学有限公司	用于治疗皮下脂肪团的组合物和方法
US20220305094A1 (en)	2019-01-06	2022-09-29	Endo Global Aesthetics Limited	Collagenase formulations and methods of producing the same
CA3157517A1 (en)	2019-10-15	2021-04-22	The Johns Hopkins University	Treatment of uterine fibroids using purified collagenase
CA3236097A1 (en)	2022-01-05	2023-07-13	Jan HEYLAND	Culture medium for cultivating hathewaya histolytica (or clostridium histolyticum) and the production of one or more proteases

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS61289885A (ja) *	1985-06-14	1986-12-19	Nitta Zerachin Kk	コラゲナ−ゼおよびコラゲナ−ゼの製造方法
JP2898022B2 (ja) *	1989-09-05	1999-05-31	株式会社ニッピ	コラーゲン分解酵素の製造方法
CA2047306A1 (en) *	1990-07-23	1992-01-24	Milan Holjevac	Mutant bacterium clostridium histolyticum, a process for the obtaining thereof, and its use in the production of clostripain-free collagenase
US5393792A (en)	1991-11-20	1995-02-28	Advance Biofactures Of Curacao, N.V.	High dosage topical forms of collagenase
US5462739A (en) *	1991-11-21	1995-10-31	Yeda Research And Development Co., Ltd.	Microdelivery device and method for enhanced drug administration to the eye
JPH08500970A (ja) *	1992-06-22	1996-02-06	トライジェン，インコーポレーテッド	ヒストリチクス菌からのコラゲナーゼ産生に応答性の遺伝子の分子クローニング
JP3186881B2 (ja) *	1993-02-18	2001-07-11	倉敷紡績株式会社	肝細胞の分離方法
US5332503A (en) *	1993-04-16	1994-07-26	Baxter International Inc.	Process for purifying collagenase
JP3028852B2 (ja) *	1994-06-24	2000-04-04	ロシュダイアグノスティックスコーポレーション	ヒストリチクス菌から得られるコラゲナーゼおよび２つの他のプロテアーゼの精製混合物
US5589171A (en)	1994-08-22	1996-12-31	Advance Biofactures Of Curacao	Treatment of Dupuytren's disease with collagenase
BR9607143A (pt) *	1995-03-16	1997-11-25	Knoll Ag	Enzima ibtenível de clostridium histolyticum mistura de enzimas uso da mistura uso de enzimas colagenase hp na forma pura colagenase az na forma pura e elastase na forma pura
US5989888A (en)	1996-01-24	1999-11-23	Roche Diagnostics Corporation	Purified mixture of collagenase I, collagenase II and two other proteases
AU5222498A (en)	1996-11-19	1998-06-10	Boehringer Mannheim Gmbh	Type i recombinant clostridium histolyticum collagenase and its use for isolating cells and cell aggregates
US5830741A (en)	1996-12-06	1998-11-03	Boehringer Mannheim Corporation	Composition for tissue dissociation containing collagenase I and II from clostridium histolyticum and a neutral protease
US6086872A (en)	1997-03-27	2000-07-11	Advance Biofactures Of Curacao, Nv	Amelioration of dupuytren's disease
JPH10262658A (ja) *	1997-03-28	1998-10-06	Kikkoman Corp	ザルコシン・オキシダーゼの結晶、その製造方法及び三次元構造
US6022539A (en)	1999-06-03	2000-02-08	Advance Biofactures Of Curacao	Amelioration of peyronie's disease
JP4266817B2 (ja) *	2001-07-02	2009-05-20	ノルドマルク・アルツナイミッテル・ゲゼルシヤフト・ミト・ベシュレンクテル・ハフツング・ウント・コンパニー・コマンデイトゲゼルシヤフト	酵素の精製方法およびその方法で製造された精製済み酵素並びに該酵素の用途
EP1462519A1 (en) *	2003-03-24	2004-09-29	Boehringer Ingelheim Austria GmbH	Method and devices for producing biomolecules
JP4205496B2 (ja) *	2003-06-19	2009-01-07	三菱化学株式会社	新規カルボニル還元酵素及びこれをコードするｄｎａ、ならびにこれらを利用した光学活性アルコールの製造方法
NZ576986A (en) *	2004-01-30	2010-12-24	Shire Pharmaceuticals Ireland Ltd	Production and purification of recombinant arylsulfatase A
US7355027B2 (en) *	2004-06-16	2008-04-08	Dynport Vaccine Company Llc	Bacillus anthracis protective antigen
PL1781697T3 (pl) *	2004-07-07	2009-10-30	H Lundbeck As	Nowa karbamylowana EPO i sposób jej wytwarzania
EP1773400A2 (en) *	2004-07-08	2007-04-18	Amgen Inc.	Therapeutic peptides
US20060204488A1 (en) *	2005-01-21	2006-09-14	Badalamente Marie A	Methods for treating adhesive capsulitis
US7811560B2 (en) *	2006-01-30	2010-10-12	Auxilium Us Holdings, Llc	Compositions and methods for treating collagen-mediated diseases

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US11879141B2 (en)	2012-01-12	2024-01-23	Endo Global Ventures	Nucleic acid molecules encoding clostridium histolyticum collagenase II and methods of producing the same
US11975054B2 (en)	2012-01-12	2024-05-07	Endo Global Ventures	Nucleic acid molecules encoding clostridium histolyticum collagenase I and methods of producing the same

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Publication number	Publication date
CN101400788A (zh)	2009-04-01
NZ569882A (en)	2012-03-30
US20110243908A1 (en)	2011-10-06
EP1987141B8 (en)	2019-03-06
EP2474320A1 (en)	2012-07-11
IL245434A (en)	2017-10-31
JP7191152B2 (ja)	2022-12-16
CA2637262C (en)	2014-08-19
IL192878A (en)	2013-03-24
US20110189153A1 (en)	2011-08-04
MX2008009756A (es)	2008-12-18
JP5309289B2 (ja)	2013-10-09
CN105999244B (zh)	2022-01-11
JP2015128447A (ja)	2015-07-16
IL291175A (en)	2022-05-01
US20140004094A1 (en)	2014-01-02
ES2709202T3 (es)	2019-04-15
DK2474321T3 (da)	2019-06-03
IL254184A0 (en)	2017-10-31
JP2009525283A (ja)	2009-07-09
US20170087229A1 (en)	2017-03-30
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JP2017006143A (ja)	2017-01-12
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EP1987141A2 (en)	2008-11-05
IL264361A (en)	2019-02-28
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KR20110046537A - 콜라겐­매개된 질병을 치료하기 위한 조성물 및 방법 - Google Patents