JPWO2021132576A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2021132576A5 JPWO2021132576A5 JP2021567680A JP2021567680A JPWO2021132576A5 JP WO2021132576 A5 JPWO2021132576 A5 JP WO2021132576A5 JP 2021567680 A JP2021567680 A JP 2021567680A JP 2021567680 A JP2021567680 A JP 2021567680A JP WO2021132576 A5 JPWO2021132576 A5 JP WO2021132576A5
- Authority
- JP
- Japan
- Prior art keywords
- region
- value
- fragmented
- fluorescent
- nucleosomes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005259 measurement Methods 0.000 claims 6
- 108010047956 Nucleosomes Proteins 0.000 claims 5
- 210000001623 nucleosome Anatomy 0.000 claims 5
- 239000002184 metal Substances 0.000 claims 4
- 238000000034 method Methods 0.000 claims 4
- 201000011510 cancer Diseases 0.000 claims 3
- 238000011528 liquid biopsy Methods 0.000 claims 3
- 206010028980 Neoplasm Diseases 0.000 claims 2
- 210000001124 body fluid Anatomy 0.000 claims 2
- 239000010839 body fluid Substances 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000007865 diluting Methods 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 108700025701 Retinoblastoma Genes Proteins 0.000 claims 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 claims 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 claims 1
- 238000001574 biopsy Methods 0.000 claims 1
- 230000002708 enhancing effect Effects 0.000 claims 1
- 238000002073 fluorescence micrograph Methods 0.000 claims 1
Claims (5)
- a)試料中プラズモン金属メソ結晶領域を有する測定基板を用い、体液または細胞を含む培養液をそのまま又は希釈して作成した検体を接触させ、検体中のアポトーシス後の断片化DNA(細分化ヌクレオソーム)を疾病関連物質としてプラズモン金属メソ結晶に電荷捕捉させる工程と、b)このプラズモン金属メソ結晶上の捕捉された断片化DNA(細分化ヌクレオソーム)に励起光を照射して、その自家蛍光を表面プラズモン増強効果により増強し、断片化DNA(細分化ヌクレオソーム)の蛍光コロニーの蛍光画像を一定の測定領域(ROI)を決め、ブルーB波長領域の蛍光コロニー画像を悪性腫瘍に関する値として取得する工程と、c)該蛍光コロニー画像の所定の閾値以上の輝度を示すピクセルを採択する工程と、d)採択された測定領域の所定のブルーB波長領域での所定の閾値以上のピクセルの総面積値を演算する工程を含むことを特徴とする自家蛍光によるリキッド・バイオプシィ法。
- (削除)
- a)試料中プラズモン金属メソ結晶領域を有する測定基板を用い、体液または細胞を含む培養液をそのまま又は希釈して作成した検体を接触させ、検体中のアポトーシス後の断片化DNA(細分化ヌクレオソーム)を疾病関連物質としてプラズモン金属メソ結晶に電荷捕捉させる工程と、b)このプラズモン金属メソ結晶上の捕捉された断片化DNA(細分化ヌクレオソーム)に励起光を照射して、その自家蛍光を表面プラズモン増強効果により増強し、断片化DNA(細分化ヌクレオソーム)の蛍光コロニーの蛍光画像を一定の測定領域(ROI)を決め、ブルーB波長領域の蛍光コロニー画像を悪性腫瘍に関する値として及びグリーンG波長領域の蛍光コロニー画像を良性腫瘍に関する値として取得する工程と、c)該蛍光コロニー画像の所定の閾値以上の輝度を示すピクセルを採択する工程と、d)採択された測定領域の所定のB波長領域およびG波長領域での所定の閾値以上のピクセルの総面積値を求め、総面積値のRatioであるB/G又はG/B値を演算する工程を含むことを特徴とする自家蛍光によるリキッド・バイオプシィ法。
- 所定の閾値以上のピクセルの総面積値のB領域/G領域のRatio値で、悪性腫瘍で1.9から2.0の幅を持ち、健常者で0.2から0.8、良性腫瘍で0.1前後である請求項3記載のリキッド・バイオプシィ法。
- 捕捉した断片化DNA(細分化ヌクレオソーム)がメチル化されたガン抑制遺伝子、高メチル化したRB遺伝子、p16以外にp14、p53等を含む請求項1記載のリキッド・バイオプシィ法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2019234330 | 2019-12-25 | ||
JP2020204585 | 2020-11-20 | ||
PCT/JP2020/048764 WO2021132576A1 (ja) | 2019-12-25 | 2020-12-25 | アポトーシスにより細分化されたヌクレオソームを標的とする自家蛍光によるリキッド・バイオプシィ法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2021132576A1 JPWO2021132576A1 (ja) | 2021-07-01 |
JPWO2021132576A5 true JPWO2021132576A5 (ja) | 2022-10-12 |
Family
ID=76574328
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2021567680A Pending JPWO2021132576A1 (ja) | 2019-12-25 | 2020-12-25 |
Country Status (10)
Country | Link |
---|---|
US (1) | US20230050766A1 (ja) |
EP (1) | EP4083223A4 (ja) |
JP (1) | JPWO2021132576A1 (ja) |
KR (1) | KR20220136354A (ja) |
CN (1) | CN114901839A (ja) |
AU (1) | AU2020411961A1 (ja) |
BR (1) | BR112022012573A2 (ja) |
CA (1) | CA3162973A1 (ja) |
MX (1) | MX2022008008A (ja) |
WO (1) | WO2021132576A1 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI818278B (zh) * | 2020-11-20 | 2023-10-11 | 日商米特奇有限公司 | 透過自體螢光的液態生檢法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5544653B2 (ja) | 2010-02-02 | 2014-07-09 | 独立行政法人産業技術総合研究所 | 抗原抗体反応の検出方法 |
JP6091417B2 (ja) | 2011-09-13 | 2017-03-08 | 国立大学法人九州大学 | 金属ナノ粒子を用いた呈色膜および呈色方法 |
US9952157B2 (en) * | 2012-07-17 | 2018-04-24 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Tissue imaging and visualization of lesions using reflectance and autofluorescence measurements |
MY193573A (en) | 2014-05-08 | 2022-10-19 | Mytech Co Ltd | Plasmonic chip for observing cancer related substances by localized surface plasmon resonance |
EP3974387A1 (en) * | 2016-03-07 | 2022-03-30 | X-Zell Inc. | Compositions and methods for identifying rare cells |
JP2021156576A (ja) * | 2018-06-21 | 2021-10-07 | 有限会社マイテック | 疾病関連タンパク結合体を標的とする自家蛍光によるリキッド・バイオプシィ法 |
-
2020
- 2020-12-25 EP EP20905211.7A patent/EP4083223A4/en active Pending
- 2020-12-25 JP JP2021567680A patent/JPWO2021132576A1/ja active Pending
- 2020-12-25 US US17/788,852 patent/US20230050766A1/en active Pending
- 2020-12-25 WO PCT/JP2020/048764 patent/WO2021132576A1/ja active Application Filing
- 2020-12-25 AU AU2020411961A patent/AU2020411961A1/en active Pending
- 2020-12-25 CA CA3162973A patent/CA3162973A1/en active Pending
- 2020-12-25 KR KR1020227024473A patent/KR20220136354A/ko unknown
- 2020-12-25 BR BR112022012573A patent/BR112022012573A2/pt unknown
- 2020-12-25 MX MX2022008008A patent/MX2022008008A/es unknown
- 2020-12-25 CN CN202080090408.6A patent/CN114901839A/zh active Pending
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Yezhelyev et al. | In situ molecular profiling of breast cancer biomarkers with multicolor quantum dots | |
Ebisch et al. | Evaluation of p16/Ki-67 dual-stained cytology as triage test for high-risk human papillomavirus-positive women | |
Xu et al. | The utility of p16 immunostaining in fine needle aspiration in p16-positive head and neck squamous cell carcinoma | |
JP5682975B2 (ja) | がん発症又はがん発症リスクの判定方法 | |
Patel et al. | Exposure profiles and human papillomavirus infection in skin cancer: an analysis of 25 genus β-types in a population-based study | |
Park et al. | Human papillomavirus and p16 detection in cervical lymph node metastases from an unknown primary tumor | |
Zhu et al. | The relationship between oral squamous cell carcinoma and human papillomavirus: a meta-analysis of a Chinese population (1994–2011) | |
JPWO2021132576A5 (ja) | ||
Kaur et al. | Recent advances in point-of-care diagnostics for oral cancer | |
JP2020089367A (ja) | プローブ試薬および該プローブ試薬を用いたfish | |
Kerr et al. | Automated extraction of formalin-fixed, paraffin-embedded tissue for high-risk human papillomavirus testing of head and neck squamous cell carcinomas using the Roche Cobas 4800 system | |
Yao et al. | Human papillomavirus (HPV) E6/E7 mRNA detection in cervical exfoliated cells: a potential triage for HPV-positive women | |
Catteau et al. | Evaluation of the oncogenic human papillomavirus DNA test with liquid‐based cytology in primary cervical cancer screening and the importance of the ASC/SIL ratio: a Belgian study | |
Švajdler Jr et al. | Origin of cystic squamous cell carcinoma metastases in head and neck lymph nodes: addition of EBV testing improves diagnostic accuracy | |
Yuan et al. | Gastric-type adenocarcinoma in situ of uterine cervix: cytological and histopathological features of two cases | |
Clavero et al. | Squamous intraepithelial lesions of the anal squamocolumnar junction: Histopathological classification and HPV genotyping | |
Bomfim-Hyppólito et al. | Cervicography as an adjunctive test to visual inspection with acetic acid in cervical cancer detection screening | |
KR20140008375A (ko) | 초미세 구조 및 분자 마커의 검출에 의한 암 스크리닝 | |
Aiad et al. | Significance of AgNORs and ki-67 proliferative markers in differential diagnosis of thyroid lesions | |
Schache et al. | Human papillomavirus testing in head and neck squamous cell carcinoma: best practice for diagnosis | |
Huang et al. | Human papillomavirus infection is not associated with laryngeal squamous cell carcinoma in Taiwan | |
Mao et al. | DNA modulates the interaction of genetically engineered DNA-binding proteins and gold nanoparticles: diagnosis of high-risk HPV infection | |
Giotakis et al. | Apoptotic and proliferative status in HPV (+) and HPV (−) inverted papilloma patients. Correlation with local recurrence and clinicopathological variables | |
Jankowski et al. | Diagnostic and management implications of basic science advances in Barrett’s esophagus | |
Matoso et al. | Prevalence and distribution of 15 high-risk human papillomavirus types in squamous cell carcinoma of the scrotum |