JPWO2021132576A5 - - Google Patents
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- JPWO2021132576A5 JPWO2021132576A5 JP2021567680A JP2021567680A JPWO2021132576A5 JP WO2021132576 A5 JPWO2021132576 A5 JP WO2021132576A5 JP 2021567680 A JP2021567680 A JP 2021567680A JP 2021567680 A JP2021567680 A JP 2021567680A JP WO2021132576 A5 JPWO2021132576 A5 JP WO2021132576A5
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- fluorescent
- nucleosomes
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- 238000005259 measurement Methods 0.000 claims 6
- 108010047956 Nucleosomes Proteins 0.000 claims 5
- 210000001623 nucleosome Anatomy 0.000 claims 5
- 239000002184 metal Substances 0.000 claims 4
- 238000000034 method Methods 0.000 claims 4
- 201000011510 cancer Diseases 0.000 claims 3
- 238000011528 liquid biopsy Methods 0.000 claims 3
- 206010028980 Neoplasm Diseases 0.000 claims 2
- 210000001124 body fluid Anatomy 0.000 claims 2
- 239000010839 body fluid Substances 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000007865 diluting Methods 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 108700025701 Retinoblastoma Genes Proteins 0.000 claims 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 claims 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 claims 1
- 238000001574 biopsy Methods 0.000 claims 1
- 230000002708 enhancing effect Effects 0.000 claims 1
- 238000002073 fluorescence micrograph Methods 0.000 claims 1
Claims (5)
- ａ）試料中プラズモン金属メソ結晶領域を有する測定基板を用い、体液または細胞を含む培養液をそのまま又は希釈して作成した検体を接触させ、検体中のアポトーシス後の断片化ＤＮＡ（細分化ヌクレオソーム）を疾病関連物質としてプラズモン金属メソ結晶に電荷捕捉させる工程と、ｂ）このプラズモン金属メソ結晶上の捕捉された断片化ＤＮＡ（細分化ヌクレオソーム）に励起光を照射して、その自家蛍光を表面プラズモン増強効果により増強し、断片化ＤＮＡ（細分化ヌクレオソーム）の蛍光コロニーの蛍光画像を一定の測定領域（ＲＯＩ）を決め、ブルーＢ波長領域の蛍光コロニー画像を悪性腫瘍に関する値として取得する工程と、ｃ）該蛍光コロニー画像の所定の閾値以上の輝度を示すピクセルを採択する工程と、ｄ）採択された測定領域の所定のブルーＢ波長領域での所定の閾値以上のピクセルの総面積値を演算する工程を含むことを特徴とする自家蛍光によるリキッド・バイオプシィ法。
- （削除）
- ａ）試料中プラズモン金属メソ結晶領域を有する測定基板を用い、体液または細胞を含む培養液をそのまま又は希釈して作成した検体を接触させ、検体中のアポトーシス後の断片化ＤＮＡ（細分化ヌクレオソーム）を疾病関連物質としてプラズモン金属メソ結晶に電荷捕捉させる工程と、ｂ）このプラズモン金属メソ結晶上の捕捉された断片化ＤＮＡ（細分化ヌクレオソーム）に励起光を照射して、その自家蛍光を表面プラズモン増強効果により増強し、断片化ＤＮＡ（細分化ヌクレオソーム）の蛍光コロニーの蛍光画像を一定の測定領域（ＲＯＩ）を決め、ブルーＢ波長領域の蛍光コロニー画像を悪性腫瘍に関する値として及びグリーンＧ波長領域の蛍光コロニー画像を良性腫瘍に関する値として取得する工程と、ｃ）該蛍光コロニー画像の所定の閾値以上の輝度を示すピクセルを採択する工程と、ｄ）採択された測定領域の所定のＢ波長領域およびＧ波長領域での所定の閾値以上のピクセルの総面積値を求め、総面積値のＲａｔｉｏであるＢ／Ｇ又はＧ／Ｂ値を演算する工程を含むことを特徴とする自家蛍光によるリキッド・バイオプシィ法。
- 所定の閾値以上のピクセルの総面積値のＢ領域／Ｇ領域のＲａｔｉｏ値で、悪性腫瘍で１．９から２．０の幅を持ち、健常者で０．２から０．８、良性腫瘍で０．１前後である請求項３記載のリキッド・バイオプシィ法。
- 捕捉した断片化ＤＮＡ（細分化ヌクレオソーム）がメチル化されたガン抑制遺伝子、高メチル化したＲＢ遺伝子、ｐ１６以外にｐ１４、ｐ５３等を含む請求項１記載のリキッド・バイオプシィ法。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019234330 | 2019-12-25 | ||
| JP2020204585 | 2020-11-20 | ||
| PCT/JP2020/048764 WO2021132576A1 (ja) | 2019-12-25 | 2020-12-25 | アポトーシスにより細分化されたヌクレオソームを標的とする自家蛍光によるリキッド・バイオプシィ法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPWO2021132576A1 JPWO2021132576A1 (ja) | 2021-07-01 |
| JPWO2021132576A5 true JPWO2021132576A5 (ja) | 2022-10-12 |
Family
ID=76574328
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021567680A Pending JPWO2021132576A1 (ja) | 2019-12-25 | 2020-12-25 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230050766A1 (ja) |
| EP (1) | EP4083223A4 (ja) |
| JP (1) | JPWO2021132576A1 (ja) |
| KR (1) | KR20220136354A (ja) |
| CN (1) | CN114901839A (ja) |
| AU (1) | AU2020411961A1 (ja) |
| BR (1) | BR112022012573A2 (ja) |
| CA (1) | CA3162973A1 (ja) |
| MX (1) | MX2022008008A (ja) |
| WO (1) | WO2021132576A1 (ja) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI818278B (zh) * | 2020-11-20 | 2023-10-11 | 日商米特奇有限公司 | 透過自體螢光的液態生檢法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5544653B2 (ja) | 2010-02-02 | 2014-07-09 | 独立行政法人産業技術総合研究所 | 抗原抗体反応の検出方法 |
| JP6091417B2 (ja) | 2011-09-13 | 2017-03-08 | 国立大学法人九州大学 | 金属ナノ粒子を用いた呈色膜および呈色方法 |
| US9952157B2 (en) * | 2012-07-17 | 2018-04-24 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Tissue imaging and visualization of lesions using reflectance and autofluorescence measurements |
| MY193573A (en) | 2014-05-08 | 2022-10-19 | Mytech Co Ltd | Plasmonic chip for observing cancer related substances by localized surface plasmon resonance |
| EP3974387A1 (en) * | 2016-03-07 | 2022-03-30 | X-Zell Inc. | Compositions and methods for identifying rare cells |
| JP2021156576A (ja) * | 2018-06-21 | 2021-10-07 | 有限会社マイテック | 疾病関連タンパク結合体を標的とする自家蛍光によるリキッド・バイオプシィ法 |
-
2020
- 2020-12-25 EP EP20905211.7A patent/EP4083223A4/en active Pending
- 2020-12-25 JP JP2021567680A patent/JPWO2021132576A1/ja active Pending
- 2020-12-25 US US17/788,852 patent/US20230050766A1/en active Pending
- 2020-12-25 WO PCT/JP2020/048764 patent/WO2021132576A1/ja active Application Filing
- 2020-12-25 AU AU2020411961A patent/AU2020411961A1/en active Pending
- 2020-12-25 CA CA3162973A patent/CA3162973A1/en active Pending
- 2020-12-25 KR KR1020227024473A patent/KR20220136354A/ko unknown
- 2020-12-25 BR BR112022012573A patent/BR112022012573A2/pt unknown
- 2020-12-25 MX MX2022008008A patent/MX2022008008A/es unknown
- 2020-12-25 CN CN202080090408.6A patent/CN114901839A/zh active Pending
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