JPWO2006062149A1 - 生体サンプル分析用プレート - Google Patents
生体サンプル分析用プレート Download PDFInfo
- Publication number
- JPWO2006062149A1 JPWO2006062149A1 JP2006546748A JP2006546748A JPWO2006062149A1 JP WO2006062149 A1 JPWO2006062149 A1 JP WO2006062149A1 JP 2006546748 A JP2006546748 A JP 2006546748A JP 2006546748 A JP2006546748 A JP 2006546748A JP WO2006062149 A1 JPWO2006062149 A1 JP WO2006062149A1
- Authority
- JP
- Japan
- Prior art keywords
- biological sample
- analysis plate
- chamber
- sample
- sample analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012472 biological sample Substances 0.000 title claims abstract description 190
- 238000004458 analytical method Methods 0.000 title claims abstract description 149
- 239000000523 sample Substances 0.000 claims abstract description 227
- 230000002093 peripheral effect Effects 0.000 claims abstract description 25
- 239000000872 buffer Substances 0.000 claims description 61
- 108090000623 proteins and genes Proteins 0.000 claims description 35
- 238000006243 chemical reaction Methods 0.000 claims description 19
- 230000005484 gravity Effects 0.000 claims description 15
- 239000012503 blood component Substances 0.000 claims description 3
- 108020004414 DNA Proteins 0.000 description 188
- 238000002347 injection Methods 0.000 description 56
- 239000007924 injection Substances 0.000 description 56
- 238000011002 quantification Methods 0.000 description 32
- 238000000034 method Methods 0.000 description 28
- 102000004169 proteins and genes Human genes 0.000 description 22
- 238000001962 electrophoresis Methods 0.000 description 21
- 238000010586 diagram Methods 0.000 description 19
- 238000013508 migration Methods 0.000 description 13
- 230000005012 migration Effects 0.000 description 13
- 239000003795 chemical substances by application Substances 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 10
- 125000003729 nucleotide group Chemical group 0.000 description 10
- 230000027455 binding Effects 0.000 description 9
- 239000006172 buffering agent Substances 0.000 description 8
- 238000005251 capillar electrophoresis Methods 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 238000012163 sequencing technique Methods 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 6
- 239000003446 ligand Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 4
- 230000004568 DNA-binding Effects 0.000 description 4
- 108010026552 Proteome Proteins 0.000 description 4
- 108020004682 Single-Stranded DNA Proteins 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005429 filling process Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 229920000178 Acrylic resin Polymers 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 239000004642 Polyimide Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 238000010256 biochemical assay Methods 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000011097 chromatography purification Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000001952 enzyme assay Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000008622 extracellular signaling Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000010230 functional analysis Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000004068 intracellular signaling Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000006174 pH buffer Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 2
- 229920001721 polyimide Polymers 0.000 description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000004853 protein function Effects 0.000 description 2
- 230000004850 protein–protein interaction Effects 0.000 description 2
- 238000012207 quantitative assay Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 238000003158 yeast two-hybrid assay Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 1
- 230000007261 regionalization Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0621—Control of the sequence of chambers filled or emptied
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0654—Lenses; Optical fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Engineering & Computer Science (AREA)
- Signal Processing (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
200 パターン
3 回転部固定用穴
4 位置決め穴
5 プレート重心
6 緩衝剤注入口
7 緩衝剤注入部
8 サンプル注入口
9 サンプル注入部
10 第1の流路
11 第2の流路
12 正電極部
13 負電極部
14 第3の流路
15 流路
16 チャンバー部(第1チャンバー)
17 流路
18 流路(第1マイクロ流路)
19 流路(第2マイクロ流路)
20 サンプル保持部(第2チャンバー)
20a 開口部
21 バッファ部(第3チャンバー)
22、50 流路
23 サンプル定量部
24 溝
25 流路
26 流路
27 フィルム
28 流路
29 流路
30 電極部
31 DNAコンジュゲート
32 DNAサンプル
33 DNAサンプル
34 空気
35 部分
36 部分
37 フィルム
40 円弧部分
80 試料供給用流路
90 緩衝剤供給用流路
A 泳動方向
以下、図1〜図14を用いて、本発明の実施の形態1による生体サンプル分析用プレートについて説明する。
以下、図1〜図14を用いて、本発明の実施の形態1による生体サンプル分析用プレートについて説明する。
200 パターン
3 回転部固定用穴
4 位置決め穴
5 プレート重心
6 緩衝剤注入口
7 緩衝剤注入部
8 サンプル注入口
9 サンプル注入部
10 第1の流路
11 第2の流路
12 正電極部
13 負電極部
14 第3の流路
15 流路
16 チャンバー部(第1チャンバー)
17 流路
18 流路(第1マイクロ流路)
19 流路(第2マイクロ流路)
20 サンプル保持部(第2チャンバー)
20a 開口部
21 バッファ部(第3チャンバー)
22、50 流路
23 サンプル定量部
24 溝
25 流路
26 流路
27 フィルム
28 流路
29 流路
30 電極部
31 DNAコンジュゲート
32 DNAサンプル
33 DNAサンプル
34 空気
35 部分
36 部分
37 フィルム
40 円弧部分
80 試料供給用流路
90 緩衝剤供給用流路
A 泳動方向
Claims (9)
- マイクロ流路が形成され、該マイクロ流路を流れる生体サンプルを、光学的に、あるいは電気化学的に分析する構成を有してなる生体サンプル分析用プレートにおいて、
該生体サンプル分析用プレートの回転中心に対して内周側に設けた第1チャンバーと、外周側に設けた第2チャンバーとが、第1マイクロ流路により連結されて、前記生体サンプルが、該第1マイクロ流路を介して前記第1チャンバーと前記第2チャンバーとの間で移動可能となっており、
前記第2チャンバーは、あらかじめ内部に空気を保有しており、該生体サンプル分析用プレートの回転により生ずる遠心力によって、前記生体サンプルが前記第1チャンバーから前記第2チャンバーに向けて移動するとき、前記第2チャンバー内の空気は該第2チャンバー内に圧縮保持される、
ことを特徴とする生体サンプル分析用プレート。 - 請求項1に記載の生体サンプル分析用プレートにおいて、
前記第2チャンバーの前記第1チャンバーと連通する開口部は、前記第2チャンバーの外壁のうちの該分析用プレートの外周側にある部分に形成されている、
ことを特徴とする生体サンプル分析用プレート。 - 請求項1に記載の生体サンプル分析用プレートにおいて、
前記第2チャンバーに連結された前記第1マイクロ流路に、さらに、第2マイクロ流路が連結されて設けられ、
前記第2チャンバーに保持される生体サンプルが、前記第1マイクロ流路、および前記第2マイクロ流路を介して移動可能である、
ことを特徴とする生体サンプル分析用プレート。 - 請求項2に記載の生体サンプル分析用プレートにおいて、
前記第2チャンバーに連結された前記第1マイクロ流路に、さらに、第2マイクロ流路が連結されて設けられ、
前記第2チャンバーに保持される生体サンプルが、前記第1マイクロ流路、および前記第2マイクロ流路を介して移動可能である、
ことを特徴とする生体サンプル分析用プレート。 - 請求項4に記載の生体サンプル分析用プレートにおいて、
前記第2マイクロ流路に連結されて、第3チャンバーが設けられ、
該第3チャンバーは、前記生体サンプル分析用プレートの回転中心から、前記第1チャンバーよりも近い位置に配置され、
前記生体サンプルが、前記第2チャンバーから前記第2マイクロ流路を介して前記第3チャンバーに移動可能である、
ことを特徴とする生体サンプル分析用プレート。 - 請求項3ないし5のいずれかに記載の生体サンプル分析用プレートにおいて、
前記第1のマイクロ流路、および前記第2のマイクロ流路により形成される試料供給用流路とその一部が接し、該接する部分でサンプル定量保持部を形成する緩衝剤供給用流路は、該生体サンプル分析用プレートの重心を中心とする円弧部分を有する、
ことを特徴とする生体サンプル分析用プレート。 - 請求項1ないし6のいずれかに記載の生体サンプル分析用プレートであって、
該生体サンプル分析用プレートは、抗原抗体反応分析用のものである、
ことを特徴とする生体サンプル分析用プレート。 - 請求項1ないし6のいずれかに記載の生体サンプル分析用プレートであって、
該生体サンプル分析用プレートは、血液成分分析用のものである、
ことを特徴とする生体サンプル分析用プレート。 - 請求項1ないし6のいずれかに記載の生体サンプル分析用プレートであって、
該生体サンプル分析用プレートは、遺伝子分析用のものである、
ことを特徴とする生体サンプル分析用プレート。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004355142 | 2004-12-08 | ||
JP2004355142 | 2004-12-08 | ||
PCT/JP2005/022525 WO2006062149A1 (ja) | 2004-12-08 | 2005-12-08 | 生体サンプル分析用プレート |
Publications (1)
Publication Number | Publication Date |
---|---|
JPWO2006062149A1 true JPWO2006062149A1 (ja) | 2008-06-12 |
Family
ID=36577972
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006546748A Pending JPWO2006062149A1 (ja) | 2004-12-08 | 2005-12-08 | 生体サンプル分析用プレート |
Country Status (5)
Country | Link |
---|---|
US (1) | US20080075632A1 (ja) |
EP (1) | EP1832872A4 (ja) |
JP (1) | JPWO2006062149A1 (ja) |
CN (1) | CN101073003B (ja) |
WO (1) | WO2006062149A1 (ja) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4597091B2 (ja) * | 2006-05-24 | 2010-12-15 | 株式会社日立ハイテクノロジーズ | 生化学分析装置及びそれに用いる検査カートリッジ |
JP2007330201A (ja) * | 2006-06-16 | 2007-12-27 | Ab Size:Kk | 細胞分取用マイクロチップ及び細胞分取方法 |
DE102008010436A1 (de) * | 2008-02-21 | 2009-09-03 | Berthold Technologies Gmbh & Co. Kg | Vorrichtung und Verfahren zur Messung von Lumineszenz und Fluoreszenz von transfizierten Zellen oder Organteilen |
DE102012202775B4 (de) * | 2012-02-23 | 2016-08-25 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Fluidikmodul, vorrichtung und verfahren zum pumpen einer flüssigkeit |
DE102012205545A1 (de) * | 2012-04-04 | 2013-10-10 | Robert Bosch Gmbh | Revolverbauteil für ein Reagenzgefäß, Reagenzgefäß-Einsetzteil und Reagenzgefäß |
GB2516675A (en) | 2013-07-29 | 2015-02-04 | Atlas Genetics Ltd | A valve which depressurises, and a valve system |
GB2516672B (en) | 2013-07-29 | 2015-05-20 | Atlas Genetics Ltd | A system and method for expelling liquid from a fluidic cartridge |
DE102013219929B4 (de) * | 2013-10-01 | 2015-07-30 | Albert-Ludwigs-Universität Freiburg | Fluidikmodul, Vorrichtung und Verfahren zum Aliquotieren einer Flüssigkeit |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004212050A (ja) * | 2002-05-08 | 2004-07-29 | Hitachi High-Technologies Corp | 化学分析装置及び遺伝子診断装置 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69422604T2 (de) * | 1994-03-08 | 2000-06-08 | Zeptosens Ag, Witterwil | Vorrichtung und Verfahren, die Bioaffinitätsassay und elektrophoretische Auftrennung kombinieren |
US20010055812A1 (en) * | 1995-12-05 | 2001-12-27 | Alec Mian | Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics |
ATE395136T1 (de) * | 1999-06-22 | 2008-05-15 | Tecan Trading Ag | Vorrichtungen zur durchfuehrung von miniaturisierten in vitro amplifizierungsassays |
DE60124699T2 (de) * | 2000-05-15 | 2007-09-13 | Tecan Trading Ag | Zweirichtungs-durchfluss-zentrifugalmikrofluid-vorrichtungen |
EP1384249A1 (en) * | 2001-03-19 | 2004-01-28 | Gyros AB | A microfluidic system (ms) |
JP3749991B2 (ja) * | 2001-10-18 | 2006-03-01 | アイダエンジニアリング株式会社 | 微量液体秤取構造及び該構造を有するマイクロチップ |
EP1304167B1 (en) * | 2001-10-18 | 2004-07-28 | Aida Engineering Ltd. | Micro-globule metering and sampling structure and microchips having the structure |
CN1477400A (zh) * | 2002-08-23 | 2004-02-25 | 汶 连 | 一种微流体***及控制微流体在微流体***中运动的方法 |
CN1482465A (zh) * | 2002-09-13 | 2004-03-17 | 上海博N微晶科技有限公司 | 变轴心微流体***及控制微流体在该***中运动的方法 |
EP1656203A2 (en) * | 2003-06-19 | 2006-05-17 | Nagaoka & Co., Ltd. | Fluidic circuits for sample preparation including bio-discs and methods relating thereto |
US20040265172A1 (en) * | 2003-06-27 | 2004-12-30 | Pugia Michael J. | Method and apparatus for entry and storage of specimens into a microfluidic device |
EP1669733B1 (en) * | 2003-10-03 | 2019-01-16 | National Institute for Materials Science | Measuring chip |
-
2005
- 2005-12-08 US US11/792,521 patent/US20080075632A1/en not_active Abandoned
- 2005-12-08 CN CN2005800421851A patent/CN101073003B/zh active Active
- 2005-12-08 WO PCT/JP2005/022525 patent/WO2006062149A1/ja active Application Filing
- 2005-12-08 EP EP05814150A patent/EP1832872A4/en not_active Withdrawn
- 2005-12-08 JP JP2006546748A patent/JPWO2006062149A1/ja active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004212050A (ja) * | 2002-05-08 | 2004-07-29 | Hitachi High-Technologies Corp | 化学分析装置及び遺伝子診断装置 |
Also Published As
Publication number | Publication date |
---|---|
EP1832872A1 (en) | 2007-09-12 |
EP1832872A4 (en) | 2009-07-29 |
WO2006062149A1 (ja) | 2006-06-15 |
US20080075632A1 (en) | 2008-03-27 |
CN101073003B (zh) | 2011-07-06 |
CN101073003A (zh) | 2007-11-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2250212C (en) | Device and method for multiple analyte detection | |
JPWO2006062149A1 (ja) | 生体サンプル分析用プレート | |
US20070154895A1 (en) | Multi-assay microfluidic chips | |
JP4646809B2 (ja) | 生体サンプル判別装置、生体サンプル判別方法、及び生体サンプル判別用プレート | |
EP3984642A1 (en) | Methods and systems for nucleic acid analysis and quantification | |
JP5298718B2 (ja) | 生体試料反応用チップに反応液を充填する遠心装置 | |
EP2684027B1 (en) | Rapid quantification of biomolecules in a selectively functionalized nanofluidic biosensor and method thereof | |
JP2006337214A (ja) | 生体サンプル判別装置、生体サンプル判別用プレートカートリッジ、及び生体サンプル判別用プレート供給装置 | |
JP2009121860A (ja) | 生体試料判別用プレート及びそれを用いた生体試料移送方法 | |
JP2006226752A (ja) | 生体サンプル判別装置用プレート | |
JP2006308447A (ja) | 生体サンプル分析用プレート及び生体サンプル分析方法 | |
US10890559B2 (en) | Method and device for accelerated surface-based reactions | |
JP4483608B2 (ja) | 蛍光分析装置 | |
JP2007075051A (ja) | 生体サンプル判別用プレート | |
JP2006349559A (ja) | 反応容器及びこれを用いた物質の検出方法 | |
US20060160210A1 (en) | Biological sample analysis plate | |
JP2006242612A (ja) | 生体サンプル判別用プレート | |
JP2006220531A (ja) | 生体サンプル判別用プレート | |
CN105874320B (zh) | 用于纳米流体生物传感器的气体排空*** | |
JP2010088317A (ja) | 生体試料定量用チップ、生体試料定量用キット、及び生体試料定量方法 | |
JP2010063395A (ja) | 生体試料反応用チップ及び生体試料反応方法 | |
JP2009058354A (ja) | 生体サンプル分析用プレート | |
JP2007121185A (ja) | 生体サンプル判別用プレートおよびその製造方法 | |
JP2008232663A (ja) | SNPs解析装置、およびSNPs解析方法 | |
JP2009192348A (ja) | 生体サンプル分析用プレートの液体定量方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20081009 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20101026 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20101227 |
|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20110613 |
|
RD05 | Notification of revocation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7425 Effective date: 20110613 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110614 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110727 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20111220 |