JPS6341424B2 - - Google Patents

Info

Publication number: JPS6341424B2
Authority: JP; Japan
Prior art keywords: pro; peptide; ser; leu; solvent
Prior art date: 1980-06-13
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP55080467A

Other languages

English (en)

Japanese (ja)

Other versions

JPS576363A (en

Inventor

Susumu Iwasa

Koichi Kondo

Isamu Yoshida

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Takeda Pharmaceutical Co Ltd

Original Assignee

Takeda Chemical Industries Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1980-06-13

Filing date

1980-06-13

Publication date

1988-08-17

1980-06-13 Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd

1980-06-13 Priority to JP8046780A priority Critical patent/JPS576363A/ja

1981-03-26 Priority to DK137181A priority patent/DK159276C/da

1981-03-28 Priority to EP83111727A priority patent/EP0111216A3/de

1981-03-28 Priority to DE8181102360T priority patent/DE3173597D1/de

1981-03-28 Priority to EP81102360A priority patent/EP0037110B1/de

1981-03-30 Priority to CA000374165A priority patent/CA1162848A/en

1982-01-13 Publication of JPS576363A publication Critical patent/JPS576363A/ja

1983-09-19 Priority to US06/533,619 priority patent/US4517290A/en

1988-08-17 Publication of JPS6341424B2 publication Critical patent/JPS6341424B2/ja

Status Granted legal-status Critical Current

Links

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QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 42
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ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
229960003272 asparaginase Drugs 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-M asparaginate Chemical compound [O-]C(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-M 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
150000001540 azides Chemical class 0.000 description 1
239000011324 bead Substances 0.000 description 1
125000001584 benzyloxycarbonyl group Chemical group C(=O)(OCC1=CC=CC=C1)* 0.000 description 1
108010019077 beta-Amylase Proteins 0.000 description 1
108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
102000006995 beta-Glucosidase Human genes 0.000 description 1
108010047754 beta-Glucosidase Proteins 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
229910021538 borax Inorganic materials 0.000 description 1
210000004899 c-terminal region Anatomy 0.000 description 1
QXJJQWWVWRCVQT-UHFFFAOYSA-K calcium;sodium;phosphate Chemical compound [Na+].[Ca+2].[O-]P([O-])([O-])=O QXJJQWWVWRCVQT-UHFFFAOYSA-K 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
150000001718 carbodiimides Chemical class 0.000 description 1
108010089934 carbohydrase Proteins 0.000 description 1
PFKFTWBEEFSNDU-UHFFFAOYSA-N carbonyldiimidazole Chemical compound C1=CN=CN1C(=O)N1C=CN=C1 PFKFTWBEEFSNDU-UHFFFAOYSA-N 0.000 description 1
239000001768 carboxy methyl cellulose Substances 0.000 description 1
235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
239000008112 carboxymethyl-cellulose Substances 0.000 description 1
239000003729 cation exchange resin Substances 0.000 description 1
229940023913 cation exchange resins Drugs 0.000 description 1
229940106157 cellulase Drugs 0.000 description 1
235000013330 chicken meat Nutrition 0.000 description 1
229940048961 cholinesterase Drugs 0.000 description 1
239000007979 citrate buffer Substances 0.000 description 1
230000009137 competitive binding Effects 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000009833 condensation Methods 0.000 description 1
230000005494 condensation Effects 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
229910052802 copper Inorganic materials 0.000 description 1
239000010949 copper Substances 0.000 description 1
238000004132 cross linking Methods 0.000 description 1
230000037029 cross reaction Effects 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
238000004108 freeze drying Methods 0.000 description 1
108010074605 gamma-Globulins Proteins 0.000 description 1
239000007863 gel particle Substances 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
239000000122 growth hormone Substances 0.000 description 1
229960000789 guanidine hydrochloride Drugs 0.000 description 1
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
230000035931 haemagglutination Effects 0.000 description 1
IKDUDTNKRLTJSI-UHFFFAOYSA-N hydrazine monohydrate Substances O.NN IKDUDTNKRLTJSI-UHFFFAOYSA-N 0.000 description 1
150000002431 hydrogen Chemical class 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
230000036039 immunity Effects 0.000 description 1
230000002163 immunogen Effects 0.000 description 1
102000018358 immunoglobulin Human genes 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000002347 injection Methods 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
230000000968 intestinal effect Effects 0.000 description 1
235000011073 invertase Nutrition 0.000 description 1
238000005342 ion exchange Methods 0.000 description 1
239000003456 ion exchange resin Substances 0.000 description 1
229920003303 ion-exchange polymer Polymers 0.000 description 1
229910052742 iron Inorganic materials 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
239000004816 latex Substances 0.000 description 1
229920000126 latex Polymers 0.000 description 1
235000019421 lipase Nutrition 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
230000004060 metabolic process Effects 0.000 description 1
XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 description 1
SOHCYNFHNYKSTM-UHFFFAOYSA-N methylsulfinylmethane;oxolane Chemical compound CS(C)=O.C1CCOC1 SOHCYNFHNYKSTM-UHFFFAOYSA-N 0.000 description 1
239000011259 mixed solution Substances 0.000 description 1
TYRGLVWXHJRKMT-QMMMGPOBSA-N n-benzyloxycarbonyl-l-serine-betalactone Chemical compound OC(=O)[C@H](C)NC(=O)OCC1=CC=CC=C1 TYRGLVWXHJRKMT-QMMMGPOBSA-N 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
229910052760 oxygen Inorganic materials 0.000 description 1
KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
CMPQUABWPXYYSH-UHFFFAOYSA-N phenyl phosphate Chemical compound OP(O)(=O)OC1=CC=CC=C1 CMPQUABWPXYYSH-UHFFFAOYSA-N 0.000 description 1
UYWQUFXKFGHYNT-UHFFFAOYSA-N phenylmethyl ester of formic acid Natural products O=COCC1=CC=CC=C1 UYWQUFXKFGHYNT-UHFFFAOYSA-N 0.000 description 1
NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical compound OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 1
229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
150000004032 porphyrins Chemical class 0.000 description 1
229910000160 potassium phosphate Inorganic materials 0.000 description 1
235000011009 potassium phosphates Nutrition 0.000 description 1
239000000583 progesterone congener Substances 0.000 description 1
108010029020 prolylglycine Proteins 0.000 description 1
125000006239 protecting group Chemical group 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
235000011083 sodium citrates Nutrition 0.000 description 1
235000011121 sodium hydroxide Nutrition 0.000 description 1
235000010339 sodium tetraborate Nutrition 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
238000007920 subcutaneous administration Methods 0.000 description 1
108060007951 sulfatase Proteins 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
230000002194 synthesizing effect Effects 0.000 description 1
125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
238000004809 thin layer chromatography Methods 0.000 description 1
AKYUZBFQLWFNOB-UHFFFAOYSA-K trisodium 2-hydroxypropane-1,2,3-tricarboxylate hydrochloride Chemical group [Na+].[Na+].[Na+].Cl.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O AKYUZBFQLWFNOB-UHFFFAOYSA-K 0.000 description 1
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Medicinal Chemistry (AREA)
Immunology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Endocrinology (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Peptides Or Proteins (AREA)

JP8046780A 1980-03-31 1980-06-13 Production of specific antibody Granted JPS576363A (en)

Priority Applications (7)

Application Number	Priority Date	Filing Date	Title
JP8046780A JPS576363A (en)	1980-06-13	1980-06-13	Production of specific antibody
DK137181A DK159276C (da)	1980-03-31	1981-03-26	Fremgangsmaade til isolering af specifikke antistoffer og enzym-immunbestemmelsesmetode med anvendelse af det isolerede antistof
EP83111727A EP0111216A3 (de)	1980-03-31	1981-03-28	Enzymimmunologisches Testverfahren, Peptid-Enzym-Konjugat, dessen Lyophilisat, Antikörper und Reagenzsatz dafür
DE8181102360T DE3173597D1 (en)	1980-03-31	1981-03-28	Specific antibody and its use in an enzyme immuno assay
EP81102360A EP0037110B1 (de)	1980-03-31	1981-03-28	Spezifischer Antikörper und dessen Verwendung in einem Enzymimmuntest
CA000374165A CA1162848A (en)	1980-03-31	1981-03-30	Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor
US06/533,619 US4517290A (en)	1980-03-31	1983-09-19	Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP8046780A JPS576363A (en)	1980-06-13	1980-06-13	Production of specific antibody

Publications (2)

Publication Number	Publication Date
JPS576363A JPS576363A (en)	1982-01-13
JPS6341424B2 true JPS6341424B2 (de)	1988-08-17

Family

ID=13719057

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP8046780A Granted JPS576363A (en)	1980-03-31	1980-06-13	Production of specific antibody

Country Status (1)

Country	Link
JP (1)	JPS576363A (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5781447A (en) *	1980-11-11	1982-05-21	Toyo Jozo Co Ltd	Human chorionic gonadotropic hormone c-terminal fragment
JPS59216826A (ja) *	1983-05-24	1984-12-06	Mitsubishi Chem Ind Ltd	抗血清
KR910003092A (ko) *	1988-07-20	1991-02-26	챨스 엠. 브록	항체 정제 방법에 사용하기 위한 hcg펩티드

Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS53101522A (en) *	1976-12-10	1978-09-05	Erba Carlo Spa	Improved enzyme binding immune analysing method
JPS5411231A (en) *	1977-06-27	1979-01-27	Daiichi Radioisotope Lab	Production of purified antiimyoglobin antibody and quantitative analysis of myoglobin

1980
- 1980-06-13 JP JP8046780A patent/JPS576363A/ja active Granted

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS53101522A (en) *	1976-12-10	1978-09-05	Erba Carlo Spa	Improved enzyme binding immune analysing method
JPS5411231A (en) *	1977-06-27	1979-01-27	Daiichi Radioisotope Lab	Production of purified antiimyoglobin antibody and quantitative analysis of myoglobin

Also Published As

Publication number	Publication date
JPS576363A (en)	1982-01-13

Publication	Publication Date	Title
US4496658A (en)	1985-01-29	Method for enzyme immunoassay and production of antibody
US4517290A (en)	1985-05-14	Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor
EP0111216A2 (de)	1984-06-20	Enzymimmunologisches Testverfahren, Peptid-Enzym-Konjugat, dessen Lyophilisat, Antikörper und Reagenzsatz dafür
JPS63109796A (ja)	1988-05-14	高純度プロコラ−ゲン・ペプチド（３型）の製法
Fuchs et al.	1971	Immunological studies of plant hormones: detection and estimation by immunological assays
EP0421392B1 (de)	1994-08-17	Monoklonaler Antikörper gegen Kern-hCG-beta, seine Herstellung und Verwendung
US5451527A (en)	1995-09-19	HCG peptides for use in antibody purification procedures
JPH0136065B2 (de)	1989-07-28
JPH0213396A (ja)	1990-01-17	選択的免疫学的定量のためのモノクローナル抗体
EP0109078B1 (de)	1988-05-04	Immunochemische Bestimmung von menschlichem Choriongonadotropin und Reagens hierfür
JPS6341424B2 (de)	1988-08-17
JPS6311626B2 (de)	1988-03-15
JPS6362698B2 (de)	1988-12-05
JPH0218836B2 (de)	1990-04-26
JP2873586B2 (ja)	1999-03-24	抗体およびこれを用いる免疫化学的測定方法
EP0088368A2 (de)	1983-09-14	Immunchemisches Nachweisverfahren und Reagenz für menschliches Choriongonadotropin
JP2507982B2 (ja)	1996-06-19	ヒト癌胎児性抗原反応性モノクロ―ナル抗体の製造法
Deftos	1992	Two-site immunoradiometric assay of intact salmon calcitonin
CA1218300A (en)	1987-02-24	Immunochemical assay of human chorionic gonadotropin and reagent therefor
JPH083490B2 (ja)	1996-01-17	ヒト癌胎児性抗原の免疫化学的測定法および試薬
JP3419746B2 (ja)	2003-06-23	エンドセリン−３前駆体に対するモノクローナル抗体およびその用途
JPH032262B2 (de)	1991-01-14
JPS58151558A (ja)	1983-09-08	ヒト絨毛性ゴナドトロピンの免疫化学的測定法および試薬
JPH0746105B2 (ja)	1995-05-17	インタ−フエロン−γの免疫化学的測定法および測定用試薬
JPWO2003099869A1 (ja)	2005-09-22	８−ニトログアニンを認識する抗体