JPH0218836B2 - - Google Patents

Info

Publication number: JPH0218836B2
Authority: JP; Japan
Prior art keywords: pro; hcg; ser; solvent; leu
Prior art date: 1981-01-14
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP56004507A

Other languages

English (en)

Japanese (ja)

Other versions

JPS57118790A (en

Inventor

Susumu Iwasa

Koichi Kondo

Isamu Yoshida

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Takeda Pharmaceutical Co Ltd

Original Assignee

Takeda Chemical Industries Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-01-14

Filing date

1981-01-14

Publication date

1990-04-26

1981-01-14 Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd

1981-01-14 Priority to JP450781A priority Critical patent/JPS57118790A/ja

1981-03-26 Priority to DK137181A priority patent/DK159276C/da

1981-03-28 Priority to EP83111727A priority patent/EP0111216A3/de

1981-03-28 Priority to DE8181102360T priority patent/DE3173597D1/de

1981-03-28 Priority to EP81102360A priority patent/EP0037110B1/de

1981-03-30 Priority to CA000374165A priority patent/CA1162848A/en

1981-10-13 Priority to DE8181108272T priority patent/DE3174064D1/de

1981-10-13 Priority to EP81108272A priority patent/EP0049898B2/de

1981-10-14 Priority to CA000387873A priority patent/CA1181342A/en

1981-10-14 Priority to US06/311,327 priority patent/US4496658A/en

1982-07-23 Publication of JPS57118790A publication Critical patent/JPS57118790A/ja

1983-09-19 Priority to US06/533,619 priority patent/US4517290A/en

1990-04-26 Publication of JPH0218836B2 publication Critical patent/JPH0218836B2/ja

Status Granted legal-status Critical Current

Links

239000000203 mixture Substances 0.000 claims description 91
108010005774 beta-Galactosidase Proteins 0.000 claims description 73
238000004519 manufacturing process Methods 0.000 claims description 33
238000004108 freeze drying Methods 0.000 claims description 20
239000000126 substance Substances 0.000 claims description 18
102000005936 beta-Galactosidase Human genes 0.000 claims description 16
235000000346 sugar Nutrition 0.000 claims description 15
150000005846 sugar alcohols Chemical class 0.000 claims description 12
238000002372 labelling Methods 0.000 claims description 7
102000009027 Albumins Human genes 0.000 claims description 6
108010088751 Albumins Proteins 0.000 claims description 6
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 147
XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 87
239000002904 solvent Substances 0.000 description 81
239000000047 product Substances 0.000 description 61
108090000765 processed proteins & peptides Proteins 0.000 description 58
WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 57
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 52
238000000034 method Methods 0.000 description 51
239000003054 catalyst Substances 0.000 description 46
102000004190 Enzymes Human genes 0.000 description 45
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ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 40
239000003153 chemical reaction reagent Substances 0.000 description 39
239000000243 solution Substances 0.000 description 39
QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 38
238000006243 chemical reaction Methods 0.000 description 34
230000000694 effects Effects 0.000 description 33
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 32
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 32
239000000872 buffer Substances 0.000 description 30
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 28
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 25
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KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 24
238000003756 stirring Methods 0.000 description 23
239000000843 powder Substances 0.000 description 22
239000001257 hydrogen Substances 0.000 description 21
229910052739 hydrogen Inorganic materials 0.000 description 21
UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 20
238000004458 analytical method Methods 0.000 description 20
239000000427 antigen Substances 0.000 description 20
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239000002244 precipitate Substances 0.000 description 20
239000011780 sodium chloride Substances 0.000 description 19
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239000012085 test solution Substances 0.000 description 18
238000002844 melting Methods 0.000 description 17
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230000002829 reductive effect Effects 0.000 description 17
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 16
PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 16
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 16
238000000921 elemental analysis Methods 0.000 description 16
229940027941 immunoglobulin g Drugs 0.000 description 13
ZUSSTQCWRDLYJA-UHFFFAOYSA-N n-hydroxy-5-norbornene-2,3-dicarboximide Chemical compound C1=CC2CC1C1C2C(=O)N(O)C1=O ZUSSTQCWRDLYJA-UHFFFAOYSA-N 0.000 description 13
239000000758 substrate Substances 0.000 description 13
238000000354 decomposition reaction Methods 0.000 description 12
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210000004900 c-terminal fragment Anatomy 0.000 description 11
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108091003079 Bovine Serum Albumin Proteins 0.000 description 9
ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 9
238000005406 washing Methods 0.000 description 9
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 8
WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 8
DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 8
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NPZTUJOABDZTLV-UHFFFAOYSA-N hydroxybenzotriazole Substances O=C1C=CC=C2NNN=C12 NPZTUJOABDZTLV-UHFFFAOYSA-N 0.000 description 8
229910001629 magnesium chloride Inorganic materials 0.000 description 8
230000036961 partial effect Effects 0.000 description 8
241000283973 Oryctolagus cuniculus Species 0.000 description 7
239000004793 Polystyrene Substances 0.000 description 7
UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 7
230000027455 binding Effects 0.000 description 7
239000001913 cellulose Substances 0.000 description 7
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230000009137 competitive binding Effects 0.000 description 7
230000000984 immunochemical effect Effects 0.000 description 7
230000000977 initiatory effect Effects 0.000 description 7
229920002223 polystyrene Polymers 0.000 description 7
102000004196 processed proteins & peptides Human genes 0.000 description 7
239000007790 solid phase Substances 0.000 description 7
HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 description 6
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
229910019142 PO4 Inorganic materials 0.000 description 6
229920002684 Sepharose Polymers 0.000 description 6
229930006000 Sucrose Natural products 0.000 description 6
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 6
235000001014 amino acid Nutrition 0.000 description 6
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150000001413 amino acids Chemical class 0.000 description 6
229910001873 dinitrogen Inorganic materials 0.000 description 6
238000006911 enzymatic reaction Methods 0.000 description 6
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 6
239000007791 liquid phase Substances 0.000 description 6
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 6
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VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
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125000003277 amino group Chemical group 0.000 description 5
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MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 description 5
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150000002500 ions Chemical class 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
238000005304 joining Methods 0.000 description 1
239000007788 liquid Substances 0.000 description 1
229910052749 magnesium Inorganic materials 0.000 description 1
159000000003 magnesium salts Chemical class 0.000 description 1
FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
239000000463 material Substances 0.000 description 1
HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
HOVAGTYPODGVJG-UHFFFAOYSA-N methyl beta-galactoside Natural products COC1OC(CO)C(O)C(O)C1O HOVAGTYPODGVJG-UHFFFAOYSA-N 0.000 description 1
SOHCYNFHNYKSTM-UHFFFAOYSA-N methylsulfinylmethane;oxolane Chemical compound CS(C)=O.C1CCOC1 SOHCYNFHNYKSTM-UHFFFAOYSA-N 0.000 description 1
239000011259 mixed solution Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
210000003205 muscle Anatomy 0.000 description 1
TYRGLVWXHJRKMT-QMMMGPOBSA-N n-benzyloxycarbonyl-l-serine-betalactone Chemical compound OC(=O)[C@H](C)NC(=O)OCC1=CC=CC=C1 TYRGLVWXHJRKMT-QMMMGPOBSA-N 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
229960001319 parathyroid hormone Drugs 0.000 description 1
239000000199 parathyroid hormone Substances 0.000 description 1
229940049954 penicillin Drugs 0.000 description 1
150000002972 pentoses Chemical class 0.000 description 1
UYWQUFXKFGHYNT-UHFFFAOYSA-N phenylmethyl ester of formic acid Natural products O=COCC1=CC=CC=C1 UYWQUFXKFGHYNT-UHFFFAOYSA-N 0.000 description 1
NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical compound OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 1
239000008055 phosphate buffer solution Substances 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
239000000186 progesterone Substances 0.000 description 1
229960003387 progesterone Drugs 0.000 description 1
108010029020 prolylglycine Proteins 0.000 description 1
125000006239 protecting group Chemical group 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
230000009257 reactivity Effects 0.000 description 1
238000005057 refrigeration Methods 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
229910052710 silicon Inorganic materials 0.000 description 1
239000010703 silicon Substances 0.000 description 1
235000011121 sodium hydroxide Nutrition 0.000 description 1
XHFLOLLMZOTPSM-UHFFFAOYSA-M sodium;hydrogen carbonate;hydrate Chemical compound [OH-].[Na+].OC(O)=O XHFLOLLMZOTPSM-UHFFFAOYSA-M 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
239000000600 sorbitol Substances 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
150000003431 steroids Chemical class 0.000 description 1
238000007920 subcutaneous administration Methods 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
238000004809 thin layer chromatography Methods 0.000 description 1
150000004043 trisaccharides Chemical class 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
229940088594 vitamin Drugs 0.000 description 1
239000011782 vitamin Substances 0.000 description 1
235000013343 vitamin Nutrition 0.000 description 1
229930003231 vitamin Natural products 0.000 description 1
239000000811 xylitol Substances 0.000 description 1
235000010447 xylitol Nutrition 0.000 description 1
HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
229960002675 xylitol Drugs 0.000 description 1
FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1

Landscapes

Enzymes And Modification Thereof (AREA)

JP450781A 1980-03-31 1981-01-14 Freeze-dried substance containing beta-d-galactosidase Granted JPS57118790A (en)

Priority Applications (11)

Application Number	Priority Date	Filing Date	Title
JP450781A JPS57118790A (en)	1981-01-14	1981-01-14	Freeze-dried substance containing beta-d-galactosidase
DK137181A DK159276C (da)	1980-03-31	1981-03-26	Fremgangsmaade til isolering af specifikke antistoffer og enzym-immunbestemmelsesmetode med anvendelse af det isolerede antistof
EP83111727A EP0111216A3 (de)	1980-03-31	1981-03-28	Enzymimmunologisches Testverfahren, Peptid-Enzym-Konjugat, dessen Lyophilisat, Antikörper und Reagenzsatz dafür
DE8181102360T DE3173597D1 (en)	1980-03-31	1981-03-28	Specific antibody and its use in an enzyme immuno assay
EP81102360A EP0037110B1 (de)	1980-03-31	1981-03-28	Spezifischer Antikörper und dessen Verwendung in einem Enzymimmuntest
CA000374165A CA1162848A (en)	1980-03-31	1981-03-30	Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor
DE8181108272T DE3174064D1 (en)	1980-10-15	1981-10-13	Method for immunochemical assay and kit therefor
EP81108272A EP0049898B2 (de)	1980-10-15	1981-10-13	Immunochemisches Testverfahren und Reagenzsatz dafür
CA000387873A CA1181342A (en)	1980-10-15	1981-10-14	Method for enzyme immunoassay and production of antibody
US06/311,327 US4496658A (en)	1980-10-15	1981-10-14	Method for enzyme immunoassay and production of antibody
US06/533,619 US4517290A (en)	1980-03-31	1983-09-19	Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP450781A JPS57118790A (en)	1981-01-14	1981-01-14	Freeze-dried substance containing beta-d-galactosidase

Publications (2)

Publication Number	Publication Date
JPS57118790A JPS57118790A (en)	1982-07-23
JPH0218836B2 true JPH0218836B2 (de)	1990-04-26

Family

ID=11585959

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP450781A Granted JPS57118790A (en)	1980-03-31	1981-01-14	Freeze-dried substance containing beta-d-galactosidase

Country Status (1)

Country	Link
JP (1)	JPS57118790A (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH0677019B2 (ja) *	1984-01-17	1994-09-28	株式会社ヤトロン	酵素標識抗体の安定化法
JPS60188067A (ja) *	1984-03-09	1985-09-25	Takeda Chem Ind Ltd	ペルオキシダ−ゼ含有組成物
JPH0728741B2 (ja) *	1985-05-14	1995-04-05	わかもと製薬株式会社	安定なβ−ガラクトシダ−ゼ水性組成物
JPH0728742B2 (ja) *	1985-08-07	1995-04-05	太陽化学株式会社	エラスタ−ゼの安定化法
JPH0728743B2 (ja) *	1985-11-08	1995-04-05	東洋紡績株式会社	安定なグアナ−ゼ組成物
ES2533617T3 (es) *	2009-06-23	2015-04-13	Nestec S.A.	Leche en polvo que contiene lactasa
JP6349452B1 (ja)	2017-11-29	2018-06-27	ヤマサ醤油株式会社	Ｌ−グルタミン酸オキシダーゼ乾燥組成物

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5217116A (en) *	1975-07-30	1977-02-08	Nippon Soken Inc	Binary fluid fuel injection valve

1981
- 1981-01-14 JP JP450781A patent/JPS57118790A/ja active Granted

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5217116A (en) *	1975-07-30	1977-02-08	Nippon Soken Inc	Binary fluid fuel injection valve

Also Published As

Publication number	Publication date
JPS57118790A (en)	1982-07-23

Publication	Publication Date	Title
US4517290A (en)	1985-05-14	Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor
US4496658A (en)	1985-01-29	Method for enzyme immunoassay and production of antibody
EP0111216A2 (de)	1984-06-20	Enzymimmunologisches Testverfahren, Peptid-Enzym-Konjugat, dessen Lyophilisat, Antikörper und Reagenzsatz dafür
US4423034A (en)	1983-12-27	Process for the preparation of antibodies
Levy et al.	1967	The synthesis of triaminoacyl-insulins and the use of the t-butyloxycarbonyl group for the reversible blocking of the amino groups of insulin
GB1570532A (en)	1980-07-02	Reagent suitable for enzyme immuno assay
Scott et al.	1974	Purification and characterization of porcine corticotrophin-like intermediate lobe peptide
HU195620B (en)	1988-06-28	Process for producing conjugates with immunogenic effect and built up from haptenes and muramylpeptides and pharmaceutics comprising these conjugates
Ravazzola et al.	1988	Localization of pancreastatin immunoreactivity in porcine endocrine cells
FUJINO et al.	1978	Synthesis of the nonacosapeptide corresponding to mammalian glucagon
JPH0218836B2 (de)	1990-04-26
US5225354A (en)	1993-07-06	Monoclonal antibodies specific for human glycoalbumin
JPS6223822B2 (de)	1987-05-25
Gold et al.	1964	The amino acid sequence of a hexapeptide containing an essential sulfhydryl group of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase
GB2062644A (en)	1981-05-28	Glucagon fragment and utility hereof
US5451527A (en)	1995-09-19	HCG peptides for use in antibody purification procedures
Shively et al.	1978	Amino-terminal sequence of a carcinoembryonic antigen-like glycoprotein isolated from the colonic lavages of healthy individuals
US20070148700A1 (en)	2007-06-28	Site-specific aminoglycoside derivatives for use in immunodiagnostic assays
JPH0136065B2 (de)	1989-07-28
EP0359428A1 (de)	1990-03-21	Protein-Derivate und Verfahren zu ihrer Herstellung
JPS6311626B2 (de)	1988-03-15
Leung et al.	1977	Colonic tumor membrane-associated glycoprotein: Isolation of antigenically-active peptides after chemical cleavage
JPS6362698B2 (de)	1988-12-05
JPS6341424B2 (de)	1988-08-17
JPS6120560B2 (de)	1986-05-22