JPS565095A - Purification of kallidinogenase - Google Patents

Purification of kallidinogenase

Info

Publication number
JPS565095A
JPS565095A JP7942579A JP7942579A JPS565095A JP S565095 A JPS565095 A JP S565095A JP 7942579 A JP7942579 A JP 7942579A JP 7942579 A JP7942579 A JP 7942579A JP S565095 A JPS565095 A JP S565095A
Authority
JP
Japan
Prior art keywords
kallidinogenase
antibody
insolubilized
crude
eluted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP7942579A
Other languages
Japanese (ja)
Inventor
Kenji Suzuki
Makoto Sato
Naohisa Ninomiya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanwa Kagaku Kenkyusho Co Ltd
Original Assignee
Sanwa Kagaku Kenkyusho Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanwa Kagaku Kenkyusho Co Ltd filed Critical Sanwa Kagaku Kenkyusho Co Ltd
Priority to JP7942579A priority Critical patent/JPS565095A/en
Publication of JPS565095A publication Critical patent/JPS565095A/en
Pending legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURPOSE: Crude kallidinogenase is adsorbend on insolubilized kallidnogenase antibody and then eluted to give purified kallidinogenase in high yield.
CONSTITUTION: An antibody which is prepared by using kallidinogenase with desired purity is made to combine covalently with an insoluble carrier to prepare insolubilized kallidinogenase antibody. The resulting antibody is mixed with crude kallidinogenase solution and they are stirred or the crude solution is passed through a column packed with the insolubilized antibody to adsorb the kallidinogenase specifically on the antibody. After the antibody is washed, kallidinogenase is eluted with an appropriate eluting agent.
COPYRIGHT: (C)1981,JPO&Japio
JP7942579A 1979-06-23 1979-06-23 Purification of kallidinogenase Pending JPS565095A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7942579A JPS565095A (en) 1979-06-23 1979-06-23 Purification of kallidinogenase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7942579A JPS565095A (en) 1979-06-23 1979-06-23 Purification of kallidinogenase

Publications (1)

Publication Number Publication Date
JPS565095A true JPS565095A (en) 1981-01-20

Family

ID=13689504

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7942579A Pending JPS565095A (en) 1979-06-23 1979-06-23 Purification of kallidinogenase

Country Status (1)

Country Link
JP (1) JPS565095A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996560A (en) * 1988-10-31 1991-02-26 Dainippon Screen Mfg. Co., Ltd. Apparatus for exposing an image of an original film on a photosensitive material in vacuum contact with the original film, and method therefor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996560A (en) * 1988-10-31 1991-02-26 Dainippon Screen Mfg. Co., Ltd. Apparatus for exposing an image of an original film on a photosensitive material in vacuum contact with the original film, and method therefor

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