JPS5537129A - Urokinase adsorbent, and preparation of high purity urokinase using the same - Google Patents

Urokinase adsorbent, and preparation of high purity urokinase using the same

Info

Publication number
JPS5537129A
JPS5537129A JP10924378A JP10924378A JPS5537129A JP S5537129 A JPS5537129 A JP S5537129A JP 10924378 A JP10924378 A JP 10924378A JP 10924378 A JP10924378 A JP 10924378A JP S5537129 A JPS5537129 A JP S5537129A
Authority
JP
Japan
Prior art keywords
urokinase
adsorbent
bonding
high purity
crude
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP10924378A
Other languages
Japanese (ja)
Other versions
JPS5737317B2 (en
Inventor
Daigaku Takiguchi
Eiji Ito
Hiroaki Nakamura
Izumi Kumita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Soda Co Ltd
Original Assignee
Nippon Soda Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Soda Co Ltd filed Critical Nippon Soda Co Ltd
Priority to JP10924378A priority Critical patent/JPS5537129A/en
Publication of JPS5537129A publication Critical patent/JPS5537129A/en
Publication of JPS5737317B2 publication Critical patent/JPS5737317B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

PURPOSE: To prepare a high purity urokinase from crude urokinase solution by affinity chromatography using a urokinase-adsorbent obtained by bonding a specific compound to a carrier.
CONSTITUTION: Crude urokinase solution is treated with a urokinase adsorbent obtained by bonding a compound I (R is amino or carboxyl; X is O, S or imino) to a water-insoluble carrier, directly or interposing a spacer. The adsorbed urokinase is eluted to obtain the objective purified urokinase. For the further improvement of the purification efficiency, the crude urokinase solution is preferably passed through a column packed with a adsorbent obtained by bonding a compound II (Y is alkylamino, alkyl-substituted quaternary ammonium or alkoxycarbonyl) and a water-insoluble carrier, before passing through the column packed with the former adsorbent.
COPYRIGHT: (C)1980,JPO&Japio
JP10924378A 1978-09-06 1978-09-06 Urokinase adsorbent, and preparation of high purity urokinase using the same Granted JPS5537129A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP10924378A JPS5537129A (en) 1978-09-06 1978-09-06 Urokinase adsorbent, and preparation of high purity urokinase using the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10924378A JPS5537129A (en) 1978-09-06 1978-09-06 Urokinase adsorbent, and preparation of high purity urokinase using the same

Publications (2)

Publication Number Publication Date
JPS5537129A true JPS5537129A (en) 1980-03-15
JPS5737317B2 JPS5737317B2 (en) 1982-08-09

Family

ID=14505235

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10924378A Granted JPS5537129A (en) 1978-09-06 1978-09-06 Urokinase adsorbent, and preparation of high purity urokinase using the same

Country Status (1)

Country Link
JP (1) JPS5537129A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58123211A (en) * 1982-01-19 1983-07-22 Fuji Electric Co Ltd Base circuit of transistor
JPH07132085A (en) * 1980-04-03 1995-05-23 Abbott Lab Preparation of plasminogen activator protein

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07132085A (en) * 1980-04-03 1995-05-23 Abbott Lab Preparation of plasminogen activator protein
JPS58123211A (en) * 1982-01-19 1983-07-22 Fuji Electric Co Ltd Base circuit of transistor

Also Published As

Publication number Publication date
JPS5737317B2 (en) 1982-08-09

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