JPS5537129A - Urokinase adsorbent, and preparation of high purity urokinase using the same - Google Patents
Urokinase adsorbent, and preparation of high purity urokinase using the sameInfo
- Publication number
- JPS5537129A JPS5537129A JP10924378A JP10924378A JPS5537129A JP S5537129 A JPS5537129 A JP S5537129A JP 10924378 A JP10924378 A JP 10924378A JP 10924378 A JP10924378 A JP 10924378A JP S5537129 A JPS5537129 A JP S5537129A
- Authority
- JP
- Japan
- Prior art keywords
- urokinase
- adsorbent
- bonding
- high purity
- crude
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
PURPOSE: To prepare a high purity urokinase from crude urokinase solution by affinity chromatography using a urokinase-adsorbent obtained by bonding a specific compound to a carrier.
CONSTITUTION: Crude urokinase solution is treated with a urokinase adsorbent obtained by bonding a compound I (R is amino or carboxyl; X is O, S or imino) to a water-insoluble carrier, directly or interposing a spacer. The adsorbed urokinase is eluted to obtain the objective purified urokinase. For the further improvement of the purification efficiency, the crude urokinase solution is preferably passed through a column packed with a adsorbent obtained by bonding a compound II (Y is alkylamino, alkyl-substituted quaternary ammonium or alkoxycarbonyl) and a water-insoluble carrier, before passing through the column packed with the former adsorbent.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10924378A JPS5537129A (en) | 1978-09-06 | 1978-09-06 | Urokinase adsorbent, and preparation of high purity urokinase using the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10924378A JPS5537129A (en) | 1978-09-06 | 1978-09-06 | Urokinase adsorbent, and preparation of high purity urokinase using the same |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5537129A true JPS5537129A (en) | 1980-03-15 |
JPS5737317B2 JPS5737317B2 (en) | 1982-08-09 |
Family
ID=14505235
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10924378A Granted JPS5537129A (en) | 1978-09-06 | 1978-09-06 | Urokinase adsorbent, and preparation of high purity urokinase using the same |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5537129A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58123211A (en) * | 1982-01-19 | 1983-07-22 | Fuji Electric Co Ltd | Base circuit of transistor |
JPH07132085A (en) * | 1980-04-03 | 1995-05-23 | Abbott Lab | Preparation of plasminogen activator protein |
-
1978
- 1978-09-06 JP JP10924378A patent/JPS5537129A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07132085A (en) * | 1980-04-03 | 1995-05-23 | Abbott Lab | Preparation of plasminogen activator protein |
JPS58123211A (en) * | 1982-01-19 | 1983-07-22 | Fuji Electric Co Ltd | Base circuit of transistor |
Also Published As
Publication number | Publication date |
---|---|
JPS5737317B2 (en) | 1982-08-09 |
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