JPS54138189A - Separation and purification of urokinase - Google Patents
Separation and purification of urokinaseInfo
- Publication number
- JPS54138189A JPS54138189A JP4571378A JP4571378A JPS54138189A JP S54138189 A JPS54138189 A JP S54138189A JP 4571378 A JP4571378 A JP 4571378A JP 4571378 A JP4571378 A JP 4571378A JP S54138189 A JPS54138189 A JP S54138189A
- Authority
- JP
- Japan
- Prior art keywords
- urokinase
- purity
- adsorbent
- bonded
- containing solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To separate and purity urokinase useful as a remedy for thrombosis and cancer, in high yield and purity, by adsorbing urokinase from a urokinase-containing solution with an adsorbent bonded with tetraaminodiphenyl sulfone.
CONSTITUTION: 3,3',4,4'-Tetraaminodiphenyl sulfone is bonded to a water-insoluble carrier which can be used as a carrier of affinity chromatography, e.g. cellulose, diatomite, etc., directly or with a spacer such as β-alanine, ω-amino acid, etc. The adsorbent thus obtained is made contact with a urokinase-containing solution such as human urine. The adsorbed urokinase is eluted, e.g. with 1M NaCl solution, and purified by, e.g. ammonium sulfate salting-out, alcohol precipitation, ultra-filtration, freeze-drying, etc.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4571378A JPS54138189A (en) | 1978-04-17 | 1978-04-17 | Separation and purification of urokinase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4571378A JPS54138189A (en) | 1978-04-17 | 1978-04-17 | Separation and purification of urokinase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS54138189A true JPS54138189A (en) | 1979-10-26 |
Family
ID=12726982
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4571378A Pending JPS54138189A (en) | 1978-04-17 | 1978-04-17 | Separation and purification of urokinase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54138189A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115386564A (en) * | 2022-09-20 | 2022-11-25 | 河南省尤里卡生物科技有限公司 | Method for purifying urokinase |
-
1978
- 1978-04-17 JP JP4571378A patent/JPS54138189A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115386564A (en) * | 2022-09-20 | 2022-11-25 | 河南省尤里卡生物科技有限公司 | Method for purifying urokinase |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2068953T3 (en) | INDOLOCARBAZOL AND ITS EMPLOYMENT. | |
JPS5740503A (en) | Separation of saccharides | |
NZ210427A (en) | Process for purifying blood clotting factor (factor viii:c); pharmaceutical compositions | |
JPS5653696A (en) | Isolation of erythropoietin by adsorption | |
KR840003422A (en) | Method of producing homogeneous human immune interferon | |
KR960004367A (en) | Preparation method of α1-antichymotrypsin | |
JPS5517364A (en) | Purification of pancreatic kallikrein | |
JPS53107479A (en) | Preparation of human lysozyme | |
JPS54138189A (en) | Separation and purification of urokinase | |
Singh et al. | Purification and characterization of a tuber lectin from Alocasia indica | |
JPS5537129A (en) | Urokinase adsorbent, and preparation of high purity urokinase using the same | |
DK0383645T3 (en) | Process for producing very high purity anti-haemophilia factor (FVIIIc) | |
FR2478084B1 (en) | NOVEL ESTERS OF TRICYCLO ACID (5.2.1.02,6) DECANE-2-CARBOXYLIC, PROCESSES FOR THEIR PREPARATION AND THEIR USE IN PERFUMERY | |
JPS557006A (en) | Purification of urokinase | |
JPS55167228A (en) | Purification of high-purity kallikrein | |
JPS565095A (en) | Purification of kallidinogenase | |
GR3004622T3 (en) | ||
JPS5750924A (en) | Purification of pancreatic enzyme, kallikrein | |
JPS557005A (en) | Protein adsorbent and purification of urokinase with it | |
EP0446850A3 (en) | Process for purifying recombinant human beta-interferon | |
JPS5668689A (en) | Method for concentration and purification of interferon | |
JPS5513002A (en) | Preparation of urokinase | |
JPS56145299A (en) | Purification of s-adenosyl-l-methionine | |
JPS54107586A (en) | Preparation of pure chondroitinase b | |
JPS5574793A (en) | Purification of kallikrein |