JPS54138189A - Separation and purification of urokinase - Google Patents

Separation and purification of urokinase

Info

Publication number
JPS54138189A
JPS54138189A JP4571378A JP4571378A JPS54138189A JP S54138189 A JPS54138189 A JP S54138189A JP 4571378 A JP4571378 A JP 4571378A JP 4571378 A JP4571378 A JP 4571378A JP S54138189 A JPS54138189 A JP S54138189A
Authority
JP
Japan
Prior art keywords
urokinase
purity
adsorbent
bonded
containing solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP4571378A
Other languages
Japanese (ja)
Inventor
Naoyoshi Okazaki
Yoshio Yamazaki
Masaaki Tanifuji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daigo Nutritive Chemicals Ltd
Takeda Pharmaceutical Co Ltd
Original Assignee
Daigo Nutritive Chemicals Ltd
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daigo Nutritive Chemicals Ltd, Takeda Chemical Industries Ltd filed Critical Daigo Nutritive Chemicals Ltd
Priority to JP4571378A priority Critical patent/JPS54138189A/en
Publication of JPS54138189A publication Critical patent/JPS54138189A/en
Pending legal-status Critical Current

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Abstract

PURPOSE: To separate and purity urokinase useful as a remedy for thrombosis and cancer, in high yield and purity, by adsorbing urokinase from a urokinase-containing solution with an adsorbent bonded with tetraaminodiphenyl sulfone.
CONSTITUTION: 3,3',4,4'-Tetraaminodiphenyl sulfone is bonded to a water-insoluble carrier which can be used as a carrier of affinity chromatography, e.g. cellulose, diatomite, etc., directly or with a spacer such as β-alanine, ω-amino acid, etc. The adsorbent thus obtained is made contact with a urokinase-containing solution such as human urine. The adsorbed urokinase is eluted, e.g. with 1M NaCl solution, and purified by, e.g. ammonium sulfate salting-out, alcohol precipitation, ultra-filtration, freeze-drying, etc.
COPYRIGHT: (C)1979,JPO&Japio
JP4571378A 1978-04-17 1978-04-17 Separation and purification of urokinase Pending JPS54138189A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4571378A JPS54138189A (en) 1978-04-17 1978-04-17 Separation and purification of urokinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4571378A JPS54138189A (en) 1978-04-17 1978-04-17 Separation and purification of urokinase

Publications (1)

Publication Number Publication Date
JPS54138189A true JPS54138189A (en) 1979-10-26

Family

ID=12726982

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4571378A Pending JPS54138189A (en) 1978-04-17 1978-04-17 Separation and purification of urokinase

Country Status (1)

Country Link
JP (1) JPS54138189A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115386564A (en) * 2022-09-20 2022-11-25 河南省尤里卡生物科技有限公司 Method for purifying urokinase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115386564A (en) * 2022-09-20 2022-11-25 河南省尤里卡生物科技有限公司 Method for purifying urokinase

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