JPS557005A - Protein adsorbent and purification of urokinase with it - Google Patents

Protein adsorbent and purification of urokinase with it

Info

Publication number
JPS557005A
JPS557005A JP7743878A JP7743878A JPS557005A JP S557005 A JPS557005 A JP S557005A JP 7743878 A JP7743878 A JP 7743878A JP 7743878 A JP7743878 A JP 7743878A JP S557005 A JPS557005 A JP S557005A
Authority
JP
Japan
Prior art keywords
urokinase
adsorbent
bonded
purified
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP7743878A
Other languages
Japanese (ja)
Other versions
JPS5834179B2 (en
Inventor
Daigaku Takiguchi
Eiji Ito
Hiroaki Nakamura
Izumi Kumita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Soda Co Ltd
Original Assignee
Nippon Soda Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Soda Co Ltd filed Critical Nippon Soda Co Ltd
Priority to JP7743878A priority Critical patent/JPS5834179B2/en
Publication of JPS557005A publication Critical patent/JPS557005A/en
Publication of JPS5834179B2 publication Critical patent/JPS5834179B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

PURPOSE: To obtain purified urokinase in high yield and purity, by adsorbing a crude urokinase solution with an adsorbent comprising a carrier bonded to an aniline derivative.
CONSTITUTION: Crude urokinase (U) is adsorbed to a packed bed of a protein adsorbent, wherein an aniline derivative of formula I: (X is alkylamino or alkyl- substituted quaternary ammonium) is bonded to a water-insoluble carrier directly or via a mediator, and then eluted with a saline solution to give purified U. The solution containing the purified U is passed through an adsorbent bonded to chloroaniline for further advantageous purification.
COPYRIGHT: (C)1980,JPO&Japio
JP7743878A 1978-06-28 1978-06-28 Protein adsorbent and method for purifying urokinase using the protein adsorbent Expired JPS5834179B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7743878A JPS5834179B2 (en) 1978-06-28 1978-06-28 Protein adsorbent and method for purifying urokinase using the protein adsorbent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7743878A JPS5834179B2 (en) 1978-06-28 1978-06-28 Protein adsorbent and method for purifying urokinase using the protein adsorbent

Publications (2)

Publication Number Publication Date
JPS557005A true JPS557005A (en) 1980-01-18
JPS5834179B2 JPS5834179B2 (en) 1983-07-25

Family

ID=13634016

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7743878A Expired JPS5834179B2 (en) 1978-06-28 1978-06-28 Protein adsorbent and method for purifying urokinase using the protein adsorbent

Country Status (1)

Country Link
JP (1) JPS5834179B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57197925U (en) * 1981-06-12 1982-12-15

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57197925U (en) * 1981-06-12 1982-12-15
JPS6223473Y2 (en) * 1981-06-12 1987-06-15

Also Published As

Publication number Publication date
JPS5834179B2 (en) 1983-07-25

Similar Documents

Publication Publication Date Title
JPS57183712A (en) Removing method of pyrogen
ES8600690A1 (en) Process for purifying factor VIII:C.
JPS5564799A (en) Multi-stage concentration and purification of interferon originated from human fibroblast
JPS5630925A (en) Purification of human growth hormone
JPS5517364A (en) Purification of pancreatic kallikrein
ES8706715A1 (en) Novel 2'-deoxy-5-substituted uridine derivatives, processes for preparing the same and antitumor agent containing the same.
JPS557005A (en) Protein adsorbent and purification of urokinase with it
EP0245875A3 (en) Method of purifying factor viii
JPS5537129A (en) Urokinase adsorbent, and preparation of high purity urokinase using the same
JPS5419965A (en) Preparation of n-substituted phenyl-3,4,5,6-tetrahydro-phthalimide
JPS5574793A (en) Purification of kallikrein
JPS55167228A (en) Purification of high-purity kallikrein
JPS5581587A (en) Purification of kallikrein
JPS565095A (en) Purification of kallidinogenase
JPS5577886A (en) Removal of kininase from kallikrein-containing liquid
JPS53127885A (en) Purification of urokinase
JPS5750924A (en) Purification of pancreatic enzyme, kallikrein
Libor et al. Binding behaviour of pyruvate carboxylase from Bacillus stearothermophilus on Sepharose—avidin
JPS5542506A (en) Enzyme adsorbent and purification with it
JPS54138189A (en) Separation and purification of urokinase
JPS52128294A (en) Separation and purification of cephalosporins
JPS557006A (en) Purification of urokinase
JPS5294485A (en) Purification of urokinase
JPS5668689A (en) Method for concentration and purification of interferon
JPS5388389A (en) Urokinase adsorbent and preparation of highly pure urokinase