JPH07101827A - Cosmetic containing concentrated phaffia pigment oil - Google Patents
Cosmetic containing concentrated phaffia pigment oilInfo
- Publication number
- JPH07101827A JPH07101827A JP5277286A JP27728693A JPH07101827A JP H07101827 A JPH07101827 A JP H07101827A JP 5277286 A JP5277286 A JP 5277286A JP 27728693 A JP27728693 A JP 27728693A JP H07101827 A JPH07101827 A JP H07101827A
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- Japan
- Prior art keywords
- pigment
- oil
- concentrated
- natural
- treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Cosmetics (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、色調、耐光性、着色性
に優れる天然赤色色素を高濃度で含有する色素油を配合
した化粧料に関するものである。詳細には、本発明は、
アスタキサンチン生産酵母ファフィア ロドチーマ（Ｐ
ｈａｆｆｉａ ｒｈｏｄｏｚｙｍａ）に含まれる色素を
抽出して得た粗抽出色素油を濃縮、脱臭処理した発色
性、耐光性、着色性に優れる天然赤色色素含有の濃縮色
素油を配合した化粧料に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition containing a pigment oil containing a high concentration of a natural red pigment excellent in color tone, light resistance and colorability. In particular, the invention is
Astaxanthin-producing yeast Phaffia rhodozyma (P
The present invention relates to a cosmetic composition containing a concentrated pigment oil containing a natural red pigment excellent in color development, light fastness and colorability, which is obtained by concentrating and deodorizing a crude extracted pigment oil obtained by extracting a pigment contained in haffia rhodozyma). .
【０００２】[0002]
【従来の技術】有機色素の中で天然色素も口紅に使用さ
れているが、汎用性はあまりない。天然色素は粉末状で
の外観が鮮やかであっても、油剤等と混合すると明度、
彩度が落ちてしまう場合が多い。また多くのものが、耐
光性が弱いため退色してしまったり、着色力も非常に弱
かったりする。また水溶性のものが多く、口紅に配合す
るのがむずかしい。汎用性の高い天然色素はエンジムシ
の乾燥粉体より抽出される赤色色素のカルミン酸、紅花
の花弁より抽出される赤色色素のカーサミンぐらいであ
る。2. Description of the Related Art Among organic pigments, natural pigments are also used for lipsticks, but they are not very versatile. Natural pigments have a bright appearance in powder form, but when mixed with an oil agent, etc.,
In many cases, the saturation will fall. In addition, many of them have a weak light resistance, and thus are discolored or have a very weak coloring power. In addition, many of them are water-soluble, and it is difficult to mix them with lipstick. The most versatile natural pigments are carminic acid, a red pigment extracted from dried powder of edible mushrooms, and carsamine, a red pigment extracted from petals of safflower.
【０００３】口紅に使用される有機色素は法律による規
制、物性による規制などいろいろと使用が制限されてい
る。さらに、実際に口紅を製造するときも注意しなけれ
ばならない問題点がある。口紅に必要な特性として、通
常保管で発汗をしないこと、色調に変化を生じないこ
と、スティック状の場合には使用時に折れることがない
こと、無害であること、刺激がないこと、いやな味がな
いことなどがあげられる。発汗に関しては、色素以外の
ワックス、オイル、酸化防止剤などの基材部分の配合割
合や組み合わせによる原因が大きい。また折れ強度につ
いても、色素の種類によっては極端に低くなることがあ
る。The use of organic dyes used in lipsticks is restricted in various ways such as regulation by law and regulation by physical properties. Further, there is a problem that must be taken into consideration when actually manufacturing the lipstick. The properties required for lipstick are that it does not sweat during normal storage, does not change color tone, does not break when used in stick form, is harmless, does not cause irritation, and has an unpleasant taste. There is no such thing. Regarding sweating, the cause is largely due to the mixing ratio and combination of base materials such as wax, oil, and antioxidant other than pigments. Also, the folding strength may be extremely low depending on the type of dye.
【０００４】口紅の色調が変化してしまう原因として、
日光、湿度、熱などがある。有機色素は無機色素に比べ
てこれらの条件に弱いものが多い。天然色素は、日光、
湿度、熱にさらに弱いものが多い。熱に関しては、製造
するときにワックス等を溶解するため長時間８０℃〜９
０℃に加熱することによることが多い。また、一般的に
有機色素は、油剤への均一な分散が非常にむずかしい。
口紅に配合する際も特定の油剤と色素で、あらかじめよ
く混練しておいてから配合する場合が多い。As a cause of changing the color tone of lipstick,
There is sunlight, humidity, heat, etc. Many organic dyes are less sensitive to these conditions than inorganic dyes. Natural pigments are sunlight,
Many are even more vulnerable to humidity and heat. With respect to heat, 80 ° C. to 9 ° C. for a long time because the wax or the like is melted during manufacturing.
Often by heating to 0 ° C. Further, it is generally very difficult for an organic dye to be uniformly dispersed in an oil agent.
When blending with lipstick, it is often the case that a specific oil and pigment are thoroughly kneaded in advance before blending.
【０００５】[0005]
【発明が解決しようとする課題】本発明は、天然色素に
基づく日光、湿度、熱に強い鮮やかな明るい赤色の化粧
料の提供を目的とする。本発明は、天然色素の特徴を損
なうことなく、これまで天然色素の欠点となっていた着
色性や安定性、色素濃度に優れた天然赤色色素を配合し
た化粧料の提供を目的としている。SUMMARY OF THE INVENTION It is an object of the present invention to provide a bright bright red cosmetic which is resistant to sunlight, humidity and heat and which is based on natural pigments. An object of the present invention is to provide a cosmetic composition containing a natural red pigment excellent in coloring property, stability and pigment concentration, which have been the drawbacks of natural pigments, without impairing the characteristics of natural pigments.
【０００６】[0006]
【課題を解決するための手段】本発明の化粧品は、天然
赤色色素アスタキサンチンを高濃度で含有している色素
油を配合する。アスタキサンチン高濃度含有色素油は、
アスタキサンチン生産酵母ファフィア ロドチーマ（Ｐ
ｈａｆｆｉａ ｒｈｏｄｏｚｙｍａ）に含まれる色素を
抽出して得た粗抽出色素油を、濃縮、脱臭処理して得た
濃縮天然赤色色素油、すなわちアスタキサンチンを主要
成分とする濃縮ファフィア色素油である。この濃縮ファ
フィア色素油は、色調、耐光性、着色性、耐熱性に優れ
るだけでなく、抗酸化活性、抗炎症作用、免疫賦活作用
等の生理活性を有する天然赤色色素アスタキサンチンを
高濃度で含有している。The cosmetic of the present invention contains a pigment oil containing a high concentration of natural red pigment astaxanthin. Astaxanthin high concentration pigment oil,
Astaxanthin-producing yeast Phaffia rhodozyma (P
It is a concentrated natural red pigment oil obtained by concentrating and deodorizing a crude extracted pigment oil obtained by extracting the pigment contained in haffia rhodozyma), that is, a concentrated Phaffia pigment oil containing astaxanthin as a main component. This concentrated Phaffia pigment oil contains not only excellent color tone, light resistance, colorability, and heat resistance, but also a high concentration of natural red pigment astaxanthin having physiological activities such as antioxidant activity, anti-inflammatory effect and immunostimulatory effect. ing.
【０００７】アスタキサンチンはβ−カロテンよりも抗
酸化作用が強くしかも、極性が高く乳化や蛋白質との複
合体の形成も容易なことから広い汎用性が考えられる。
また、アスタキサンチンはβ−カロテンに比べプロビタ
ミン活性も低く、ビタミンＡ過剰症の副作用の可能性も
低いうえ、抗菌、抗カビ性、細胞毒性も非常に低いとい
う特性も合わせ持っている。機能性天然色素として種々
の分野での利用が期待されている天然色素である。Astaxanthin has a stronger antioxidative effect than β-carotene, and since it has a high polarity and is easy to emulsify and form a complex with a protein, it is considered to have wide versatility.
In addition, astaxanthin has lower provitamin activity than β-carotene, less possibility of side effect of hypervitaminosis A, and also has properties of extremely low antibacterial, antifungal and cytotoxic properties. It is a natural pigment that is expected to be used in various fields as a functional natural pigment.
【０００８】また、ファフィア色素の主要成分であるア
スタキサンチンはβ−カロテン等のカロテノイド系色素
の中でも最も赤色色調が高く、熱、光、ｐＨ等にたいす
る安定性も比較的良好である上、カニ、エビ、サケ等の
生産食品から日常的に摂取しており安全性の点から問題
がない。本発明により、このように高い機能性を有する
アスタキサンチンを高濃度で含有した化粧品を初めて提
供することができる。濃縮天然赤色色素油はそのままの
形で使用することができる。必要な場合は乳化物の形で
あるいは粉末の形で使用することができる。これらの色
素油は化粧品においては着色剤として使用される。Further, astaxanthin, which is a main component of the Phaffia dye, has the highest red color tone among carotenoid dyes such as β-carotene and has relatively good stability against heat, light, pH and the like, and also has crab and shrimp. There is no problem in terms of safety because it is taken daily from food products such as salmon. According to the present invention, it is possible to provide for the first time a cosmetic product containing astaxanthin having such high functionality in a high concentration. The concentrated natural red pigment oil can be used as it is. If desired, they can be used in emulsion form or in powder form. These pigment oils are used as colorants in cosmetics.
【０００９】天然赤色色素アスタキサンチンを高濃度で
含有している色素油である上記濃縮天然赤色色素油の製
造方法の概略は、以下の通りである。赤色色素を生産す
るファフィア酵母を特定の抽出溶剤を用いて抽出処理
し、次いで得た粗抽出色素油を濃縮処理し、必要により
さらに脱臭処理することにより濃縮天然赤色色素油を製
造する。上記特定の抽出溶剤としてヘキサンとエタノー
ルの混液が使用される。抽出溶剤としてヘキサンとエタ
ノールの混合溶剤を採用しそれを用いてファフィア酵母
よりアスタキサンチンを抽出し、各種濃縮処理を行い、
さらに脱臭処理を行う。The outline of the method for producing the concentrated natural red pigment oil, which is a pigment oil containing a high concentration of the natural red pigment astaxanthin, is as follows. A concentrated natural red pigment oil is produced by subjecting Phaffia yeast producing a red pigment to an extraction treatment with a specific extraction solvent, and then subjecting the obtained crude extracted pigment oil to a concentration treatment and, if necessary, a further deodorizing treatment. A mixed solution of hexane and ethanol is used as the specific extraction solvent. Adopting a mixed solvent of hexane and ethanol as an extraction solvent and using it to extract astaxanthin from Phaffia yeast, perform various concentration treatments,
Further, deodorization processing is performed.
【００１０】上記濃縮処理手段としては、好ましくは低
温分別処理、ケン化処理、又は低温分別処理とケン化処
理の併用が採用される。低温分別処理とケン化処理を併
用するとき、低温分別処理をし、次いでケン化処理をす
る方法、低温分別処理とケン化処理を同時にする方法が
採用される。上記ケン化処理としては、好ましくは脂肪
酸のアルカリセッケン形成法を応用したもの又は脂肪酸
の金属セッケン形成法を応用したものが採用される。上
記脱臭処理としては、好ましくは水蒸気蒸留が採用され
る。As the concentration treatment means, low temperature fractionation treatment, saponification treatment, or a combination of low temperature fractionation treatment and saponification treatment is preferably adopted. When the low temperature fractionation treatment and the saponification treatment are used together, a method of performing the low temperature fractionation treatment and then the saponification treatment, and a method of simultaneously performing the low temperature fractionation treatment and the saponification treatment are adopted. As the saponification treatment, it is preferable to apply a method of forming a fatty acid alkali soap or a method of applying a fatty acid metal soap. As the above deodorizing treatment, steam distillation is preferably adopted.
【００１１】原料酵母として用いる赤色色素を生産する
ファフィア酵母は、微生物学分類上不完全菌類に属し赤
色色素を生産する酵母、例えばファフィア ロドチーマ
（Ｐｈａｆｆｉａ ｒｈｏｄｏｚｙｍａ）である。抽出
に用いるファフィア酵母は培養後、遠心分離やろ過によ
り集めよく水洗したのち、そのままあるいは乾燥後、ボ
ールミル、凍結粉砕、圧縮粉砕、超音波処理等の物理的
破砕処理かセルラーゼ等を用いた酵素的処理により前処
理することが望ましい。湿菌体から有機溶剤を用いて色
素油を抽出した場合、夾雑物質の混入が著しく、また、
抽出率も一様に低下することから工業的には乾燥菌体か
ら溶剤抽出する方が一般的である。The Phaffia yeast which produces a red pigment used as a raw material yeast is a yeast which belongs to an incomplete fungus in terms of microbiological classification and produces a red pigment, for example, Phaffia rhodozyma. Phaffia yeast used for extraction is collected by centrifugation or filtration after culturing, then thoroughly washed with water, and then either as it is or after drying, is physically crushed by ball mill, freeze crushing, compression crushing, ultrasonic treatment, or enzymatically using cellulase. Pretreatment by treatment is desirable. When the pigment oil is extracted from the wet cells using an organic solvent, contaminants are significantly mixed, and
Since the extraction rate also decreases uniformly, it is industrially more common to perform solvent extraction from dried cells.
【００１２】ファフィア酵母から色素を抽出するときに
用いる抽出溶剤としてはアセトン、酢酸エチル、ヘキサ
ン、エタノール等が考えられる。抽出率の良いアセトン
や酢酸エチルは、食品添加物の抽出には用いることがで
きない。また、ヘキサンは価格が低い反面抽出率の点で
問題があり、エタノールは抽出率はよいものの抽出コス
トの増加や高極性成分の増加による濃縮コストの増加の
問題がある。これらの問題を解決する手段として、ヘキ
サンの短所をエタノールを混合することにより改善し安
価で優れた食品用濃縮天然赤色色素油用の抽出溶剤とす
る。Acetone, ethyl acetate, hexane, ethanol and the like can be considered as the extraction solvent used when the pigment is extracted from Phaffia yeast. Acetone and ethyl acetate, which have a good extraction rate, cannot be used to extract food additives. Hexane is low in price, but has a problem in terms of extraction rate, and ethanol has a good extraction rate, but has a problem of increase in extraction cost and concentration cost due to increase in highly polar components. As a means for solving these problems, the disadvantages of hexane are improved by mixing with ethanol to provide an inexpensive and excellent extractant for concentrated natural red dye oil for food.
【００１３】ファフィア酵母からアスタキサンチン色素
を抽出するに当たり破砕したファフィア酵母からヘキサ
ンおよびエタノールの混合比率を適宜選ぶことによりア
スタキサンチンをより効率よく抽出することができる。
ヘキサンおよびエタノールの混合比率はエタノールの割
合が高くなるほど抽出率は向上する。しかしながら、エ
タノールの割合が増加すれば抽出コストの増加と高極性
物質が増加し、その後の濃縮操作が煩雑となる。したが
って、混合比率としては色素の抽出率、抽出コスト、ヘ
キサンとエタノールの共沸組成等から判断した。ヘキサ
ン：エタノールは９０〜５０：１０〜５０、好ましくは
８５〜８０：１５〜２０の割合で使用される。抽出方法
は抽出溶剤と乾燥菌体を撹拌混合した処理液をポンプに
て撹拌しながら圧縮粉砕機へ送液することによりよりス
ムーズに処理を行うことができる。破砕処理は抽出に応
じて複数回行うことが望ましい。When extracting the astaxanthin pigment from Phaffia yeast, astaxanthin can be extracted more efficiently by appropriately selecting the mixing ratio of hexane and ethanol from the crushed Phaffia yeast.
As for the mixing ratio of hexane and ethanol, the extraction ratio improves as the ratio of ethanol increases. However, if the proportion of ethanol increases, the extraction cost increases and the amount of highly polar substances increases, and the subsequent concentration operation becomes complicated. Therefore, the mixing ratio was judged from the extraction ratio of the dye, the extraction cost, the azeotropic composition of hexane and ethanol, and the like. Hexane: ethanol is used in a ratio of 90 to 50:10 to 50, preferably 85 to 80:15 to 20. As for the extraction method, the treatment can be carried out more smoothly by feeding the treatment liquid obtained by stirring and mixing the extraction solvent and the dry cells to the compression grinder while stirring with a pump. It is desirable to perform the crushing process multiple times depending on the extraction.
【００１４】ファフィア酵母における脂質組成で主要と
なるのはトリグリセリドと遊離脂肪酸で抽出色素油の色
素濃度を上げるためにはこれら主要成分の効率的な除去
が必要となる。主要成分の１つであるトリグリセリドは
低温分別により効率的に除ける。低温分別は−６０℃か
ら１０℃、好ましくは−５０℃でヘキサン、アセトン、
エタノール等の有機溶剤を用いて行うことができ、アス
タキサンチン類を濃縮精製することができる。例えば、
原料酵母としてトリグリセリドを特に多く含むものを使
用する場合、低温分別が優れた濃縮処理であるといえ
る。Triglycerides and free fatty acids play a major role in the lipid composition in Phaffia yeast, and efficient removal of these major components is necessary in order to increase the pigment concentration of the extracted pigment oil. Triglyceride, which is one of the main components, can be removed efficiently by low temperature fractionation. Low temperature fractionation is performed at -60 ° C to 10 ° C, preferably at -50 ° C with hexane, acetone,
It can be performed using an organic solvent such as ethanol, and astaxanthins can be concentrated and purified. For example,
When a material yeast containing a large amount of triglyceride is used, it can be said that low temperature fractionation is an excellent concentration treatment.
【００１５】主要成分の１つである遊離脂肪酸はケン化
処理により効率的に除ける。ケン化処理は水酸化ナトリ
ウム、水酸化カリウム、水酸化カルシウム等のアルカリ
を添加することにより生成する脂肪酸のアルカリ石鹸を
除き、アスタキサンチン類を濃縮精製することができ
る。例えば、原料酵母として遊離脂肪酸を特に多く含む
ものを使用する場合、ケン化処理が優れた濃縮処理であ
るといえる。ケン化処理はアセトン、ヘキサン等の有機
溶媒や油脂中で行うことができるが、もっとも効率的な
のはアセトン中で行うときである。Free fatty acid, which is one of the main components, can be efficiently removed by saponification treatment. In the saponification treatment, astaxanthins can be concentrated and purified by removing an alkaline soap of a fatty acid produced by adding an alkali such as sodium hydroxide, potassium hydroxide or calcium hydroxide. For example, when a raw material yeast containing a large amount of free fatty acids is used, the saponification treatment can be said to be an excellent concentration treatment. The saponification treatment can be carried out in an organic solvent such as acetone or hexane or a fat and oil, but the most efficient one is in acetone.
【００１６】低温分別による濃縮は遊離脂肪酸の除去率
が約４０％程度であり、また、セッケン形成法を利用し
た濃縮法では脂肪酸のみを選択的に除くことから、これ
らの方法を併用することにより、主要脂質成分が複数で
ある粗抽出色素油からより高度に色素を濃縮することが
できる。In the concentration by low temperature fractionation, the removal rate of free fatty acids is about 40%, and in the concentration method utilizing the soap forming method, only the fatty acids are selectively removed. Therefore, by combining these methods, , It is possible to concentrate pigments to a higher degree from crude extract pigment oils that have multiple major lipid components.
【００１７】また、濃縮天然赤色色素油の用途は化粧品
であり、酵母臭の除去、脱溶剤のために脱臭処理を行う
ことが必須である。脱臭処理手段としては水蒸気蒸留が
採用される。水蒸気蒸留は好ましくは通常１００〜２０
０℃、好ましくは１２０〜１５０℃、真空度は３０ｔｏ
ｒｒ以下で行われる。ファフィア酵母から抽出した色素
油には、一般に発酵生産物特有の刺激臭が存在する。こ
の刺激臭は色素を高濃度で用いた場合に特にひどく、色
素油の利用範囲を著しく限定することになる。The concentrated natural red pigment oil is used for cosmetics, and it is essential to carry out a deodorizing treatment for removing yeast odor and solvent removal. Steam distillation is used as a deodorizing means. Steam distillation is usually 100 to 20
0 ° C, preferably 120 to 150 ° C, vacuum degree is 30 to
It is performed below rr. Dye oil extracted from Phaffia yeast generally has an irritating odor peculiar to fermentation products. This pungent odor is particularly severe when the dye is used at a high concentration, and it significantly limits the range of use of the dye oil.
【００１８】この発酵臭は、ファフィア酵母を培養する
ときに用いる培地に由来することから、培養終了後に集
めた菌体を良く水洗することによりかなり軽減するが、
これだけでは色素油の脱臭は不十分である。また、抽出
溶剤に用いるヘキサンおよび濃縮時に用いるアセトン
は、最終生産物である色素油に残留してはいけないこと
になっている。従って、抽出後の色素油をさらに通常１
００〜２００℃、好ましくは１２０〜１５０℃、真空度
は３０ｔｏｒｒ以下で水蒸気残留を行うことにより発酵
臭は著しく低下し、さらに色素油中から抽出、精製溶剤
であるヘキサン、アセトンを水との共沸により完全に除
くことができる。Since this fermentation odor is derived from the medium used for culturing Phaffia yeast, it is considerably reduced by thoroughly washing the collected bacterial cells with water.
This alone is insufficient to deodorize the pigment oil. Further, hexane used as an extraction solvent and acetone used for concentration should not remain in the dye oil as the final product. Therefore, the pigment oil after extraction is usually added to 1
Fermentation odor is remarkably reduced by steam remaining at 0 to 200 ° C., preferably 120 to 150 ° C. and a vacuum degree of 30 torr or less, and hexane and acetone, which are extracted from the pigment oil and purified solvents, are used together with water. It can be completely removed by boiling.
【００１９】上記濃縮天然赤色色素油は必要により、乳
化して乳化物の形で、あるいは粉末化して粉末の形で使
用される。乳化物は上記濃縮天然赤色色素油に化粧品用
に許可されている乳化剤を添加し撹拌し、乳化物とする
ことにより製造される。このようにして得られた乳化物
はＯ／Ｗ型のエマルジョンであり、これを噴霧乾燥する
ことによって、粉末化することができる。If necessary, the concentrated natural red pigment oil is emulsified and used in the form of an emulsion, or powdered and used in the form of powder. The emulsion is produced by adding an emulsifier approved for cosmetics to the concentrated natural red dye oil and stirring the mixture to form an emulsion. The emulsion thus obtained is an O / W type emulsion, and can be pulverized by spray drying.
【００２０】[0020]
【実施例】本発明の詳細を実施例で説明する。本発明は
実施例によってなんら限定されることはない。 抽出例１ ファフィア ロドチーマ（Ｐｈａｆｆｉａ ｒｈｏｄｏ
ｚｙｍａ）に属する菌株を培養し、得られた培養物を遠
心分離やろ過により集め、スプレードライヤーにより乾
燥菌体を得た。この乾燥菌体各３００ｇにエタノール、
ヘキサン、アセトン、酢酸エチル、ヘキサンとエタノー
ルの各比率の混合溶剤３Ｌを加え撹拌混合しながら圧縮
粉砕機（ＡＰＶ ＧＡＵＬＩＮ社製 １５ＭＲ）を用い
圧縮粉砕を行った。結果を表１（溶剤による色素抽出
率）に示した。The details of the present invention will be described with reference to examples. The present invention is in no way limited by the examples. Extraction Example 1 Phaffia rhodoma
The strains belonging to Zyma) were cultured, the obtained cultures were collected by centrifugation and filtration, and dried bacterial cells were obtained by a spray dryer. Ethanol,
Hexane, acetone, ethyl acetate, and 3 L of a mixed solvent of each ratio of hexane and ethanol were added, and the mixture was stirred and mixed, and compression pulverization was performed using a compression pulverizer (15MR, manufactured by APV GAULIN). The results are shown in Table 1 (dye extraction rate by solvent).
【００２１】[0021]
【表１】 [Table 1]
【００２２】アセトン、酢酸エチルは食用色素の抽出に
は不適当であるが、どちらも抽出率は９０％以上と良好
であることから、他の利用分野では十分利用可能であ
る。ヘキサンのみの色素抽出率は６５．８％と低いがエ
タノールの含有率が高くなるにつれ、ほぼ直線的に抽出
率が向上しエタノール含有率が１５％のとき８７％とな
った。抽出率が９０％以上になるにはエタノール含有率
７５％以上でなくてはならないが、アスタキサンチン類
以外の高極性の成分の割合が増加し、その後の処理が煩
雑となることや、ヘキサンとエタノールの共沸組成比か
らヘキサン：エタノール＝８５：１５が最も望ましい値
であることが明らかとなった。Acetone and ethyl acetate are not suitable for the extraction of food dyes, but both have good extraction ratios of 90% or more, and thus can be sufficiently used in other fields of application. The dye extraction rate of hexane alone was as low as 65.8%, but as the content of ethanol increased, the extraction rate improved almost linearly to 87% when the ethanol content was 15%. The ethanol content must be 75% or more for the extraction rate to be 90% or more, but the proportion of highly polar components other than astaxanthins increases, and subsequent processing becomes complicated, and coexistence of hexane and ethanol From the boiling composition ratio, it was revealed that hexane: ethanol = 85: 15 was the most desirable value.
【００２３】濃縮天然赤色色素油の製造例１ 粗色素油から遊離脂肪酸、トリグリセリドを除くため
の、低温分別処理を行った。粗抽出色素油に１０，５，
３倍量のアセトンあるいはｎ−ヘキサン／エタノール
（８５：１５）を加え、−５０℃、１時間の撹拌を行っ
た後、４Ａ濾紙（ＡＤＢＡＮＴＥＣ製）で濾別し、濾液
を減圧濃縮して脂質量、カロテノイド量を求めた。添加
する溶剤が抽出油の３倍のときは、溶液中の沈殿物の濃
度が濃く、低温分別中に固まり分別が不可能であった
が、５倍量のアセトンにより低温分別を行うことにより
トリグリセリド、ステロール、リン脂質が除かれ色素濃
度は約１．５倍に濃縮された。その結果を表２（低温分
別処理による色素油の濃縮）に示す。Production Example 1 of Concentrated Natural Red Dye Oil A low temperature fractionation treatment was carried out to remove free fatty acids and triglycerides from crude dye oil. Crude extract dye oil 10,5
Three times the amount of acetone or n-hexane / ethanol (85:15) was added, and the mixture was stirred at -50 ° C for 1 hour, filtered with 4A filter paper (ADBANTEC), and the filtrate was concentrated under reduced pressure to prepare lipids. The amount and carotenoid amount were determined. When the amount of solvent added was 3 times that of the extracted oil, the concentration of precipitates in the solution was so high that it could not be fractionated because it solidified during low temperature fractionation, but triglyceride was obtained by performing low temperature fractionation with 5 volumes of acetone. , Sterols and phospholipids were removed, and the dye concentration was concentrated about 1.5 times. The results are shown in Table 2 (concentration of pigment oil by low temperature fractionation treatment).
【００２４】[0024]
【表２】 [Table 2]
【００２５】濃縮天然赤色色素油の製造例２ 粗色素油中の遊離脂肪酸と当量の水酸化カリウムを０．
８％含水アセトン２０ｍｌに４０℃で撹拌溶解させ、１
ｇの色素油（遊離脂肪酸７５．３９％）を添加し、室温
で１０分間撹拌しながらカリウムセッケンを形成させ
た。金属セッケン形成のため、遊離脂肪酸の４倍当量の
塩化カルシュウムを粉末のまま添加し室温で金属石鹸生
成まで十分撹拌しろ過した。ろ液を減圧濃縮して得られ
た油状物質に残存する脂肪酸と色素の回収率を求めた結
果を表３（色素油中の脂肪酸の除去）に示した。遊離脂
肪酸の９０％以上が除かれ、色素濃度は２．９倍に濃縮
された。Production Example 2 of Concentrated Natural Red Dye Oil The free fatty acid and the equivalent amount of potassium hydroxide in the crude dye oil were adjusted to 0.
Stir to dissolve in 20 ml of 8% hydrous acetone at 40 ° C., and
g oil (75.39% free fatty acid) was added and potassium soap was formed with stirring for 10 minutes at room temperature. In order to form a metal soap, calcium chloride in an amount equivalent to 4 times the free fatty acid was added as a powder, and the mixture was sufficiently stirred at room temperature until metal soap was formed and filtered. The results of obtaining the recovery rates of fatty acids and pigments remaining in the oily substance obtained by concentrating the filtrate under reduced pressure are shown in Table 3 (removal of fatty acids from pigment oil). 90% or more of the free fatty acids were removed, and the pigment concentration was concentrated 2.9 times.
【００２６】[0026]
【表３】 [Table 3]
【００２７】濃縮天然赤色色素油の製造例３ 色素油 （遊離脂肪酸：７５．３９％）１ｇを２０ｍｌ
のアセトンに溶解し、色素油中の遊離脂肪酸と当量の水
酸化カリウムを添加し、脂肪酸が十分に沈殿するまで撹
拌し、生成したカリウムセッケンをろ過し濃縮を行っ
た。ろ液からアセトンを減圧留去し得られた油状物質に
残存する脂肪酸と色素の回収率を求めた。表４（色素油
中の脂肪酸の除去）に示すように、遊離脂肪酸は９２．
１％除かれ色素回収は９０．２％と向上し、色素濃度は
２．８倍となった。Production Example 3 of concentrated natural red dye oil 20 ml of 1 g of dye oil (free fatty acid: 75.39%)
Was dissolved in acetone, the amount of potassium hydroxide equivalent to the free fatty acid in the pigment oil was added, the mixture was stirred until the fatty acid was sufficiently precipitated, and the potassium soap produced was filtered and concentrated. Acetone was distilled off from the filtrate under reduced pressure, and the recovery rate of fatty acid and dye remaining in the oily substance obtained was obtained. As shown in Table 4 (removal of fatty acids in pigment oil), the free fatty acids were 92.
By removing 1%, the dye recovery was improved to 90.2%, and the dye concentration was 2.8 times.
【００２８】アルカリ処理による抽出色素油の脂質組
成、脂肪酸組成、色素組成への影響を表５（濃縮処理に
よる組成変化）に示した。アルカリ処理により脂質では
脂肪酸が選択的に除かれ、遊離脂肪酸ではパルミチン酸
の除去率が最も高く、ついでオレイン酸、リノール酸の
順であった。色素組成ではアスタキサンチンの割合に変
化はなく、アルカリ処理の影響はなかった。The effects of the alkali treatment on the lipid composition, fatty acid composition, and pigment composition of the extracted pigment oil are shown in Table 5 (composition change due to concentration treatment). Fatty acids were selectively removed from lipids by alkali treatment, and palmitic acid was most removed from free fatty acids, followed by oleic acid and linoleic acid. There was no change in the astaxanthin ratio in the dye composition, and there was no effect of alkali treatment.
【００２９】[0029]
【表４】 [Table 4]
【００３０】[0030]
【表５】 [Table 5]
【００３１】同様の処理をヘキサン、ヘキサン：エタノ
ール（８５：１５）、エタノールにて行い、その脂肪回
収率、色素回収率を求めたところ、表６（ヘキサン、エ
タノール系での色素油中の脂肪酸の除去）に示すよう
に、これらの溶剤の脂質の除去率、色素の回収率はアセ
トンと比べ著しく低く、その濃縮効果はみられなかっ
た。The same treatment was carried out with hexane, hexane: ethanol (85:15), and ethanol, and the fat recovery rate and the dye recovery rate were determined. Table 6 (fatty acids in dye oil in hexane / ethanol system) As shown in (1), the removal rate of lipids and the recovery rate of dyes of these solvents were significantly lower than those of acetone, and the concentration effect was not observed.
【００３２】[0032]
【表６】 [Table 6]
【００３３】濃縮天然赤色色素油の製造例４ アルカリ処理と低温分別を連続して、あるいは同時に行
った場合を示す。連続処理は粗抽出色素油１０ｇを５倍
量のアセトンに溶解し、色素油中の遊離脂肪酸と当量の
水酸化カリウムを加え、カリウムセッケンが十分に生成
するまで撹拌した後、一度カリウムセッケンを濾別し、
さらに−５０℃で１時間低温分別を行った。同時処理
は、粗抽出色素油１０ｇに５倍量のアセトンを加え、色
素油中の遊離脂肪酸と当量の水酸化カリウムを添加し、
−５０℃にて１時間の低温分別を行った。アルカリ処理
に続いて低温分別をした場合、アルカリ処理で残った少
量の遊離脂肪酸、トリグリセリド、ステロールなどが除
かれ、色素は約４．４倍に濃縮された。２つの処理を同
時に行った場合でも色素は４．２倍に濃縮された。アル
カリ処理と低温分別の連続処理と同時処理の結果を表７
に示す。Production Example 4 of Concentrated Natural Red Dye Oil The following shows the case where alkali treatment and low temperature fractionation were carried out continuously or simultaneously. For continuous treatment, 10 g of crude extracted pigment oil was dissolved in 5 times the amount of acetone, potassium hydroxide equivalent to the free fatty acid in the pigment oil was added, and the mixture was stirred until potassium soap was sufficiently formed, and then potassium soap was filtered once. Aside,
Furthermore, low temperature fractionation was performed at -50 ° C for 1 hour. Simultaneous treatment was carried out by adding 5 times the amount of acetone to 10 g of crude extracted pigment oil, adding free fatty acid in the pigment oil and an equivalent amount of potassium hydroxide,
Low temperature fractionation was performed at -50 ° C for 1 hour. When the low temperature fractionation was performed after the alkali treatment, a small amount of free fatty acid, triglyceride, sterol, etc. remaining after the alkali treatment were removed, and the dye was concentrated about 4.4 times. The dye was 4.2 times more concentrated when the two treatments were performed simultaneously. Table 7 shows the results of continuous treatment and simultaneous treatment of alkali treatment and low temperature fractionation.
Shown in.
【００３４】[0034]
【表７】 [Table 7]
【００３５】濃縮天然赤色色素油の製造例５ 色素製造例４の連続処理により得られた濃縮色素油約２
０ｋｇを下記条件で水蒸気残留を行った。 真空度：３０Ｔｏｒｒ 温度：塔頂 ９６℃，塔底 １４０℃ 蒸気量：５ｋｇ／ｈｒ フィード量：２０ｋｇ／ｈｒ 残存溶剤は、水蒸気蒸留前に０．３％であったものが完
全に除去されていた。また、脱臭効果も官能的評価では
利用に当たって全く問題の無いレベルまで低下してい
た。水蒸気蒸留は比較的高温で行うため色素の損失が懸
念されるが、水蒸気との向流接触であることから高温下
の滞留時間が短いため処理前後の色素量の減少はほとん
ど認められなかった。Production Example 5 of concentrated natural red pigment oil Concentrated pigment oil obtained by continuous treatment of Pigment Production Example 4 About 2
0 kg was left under steam under the following conditions. Vacuum degree: 30 Torr Temperature: Tower top 96 ° C., Tower bottom 140 ° C. Vapor amount: 5 kg / hr Feed amount: 20 kg / hr The residual solvent was completely removed from 0.3% before the steam distillation. . In addition, the deodorizing effect was lowered to a level at which there was no problem in use by sensory evaluation. Since steam distillation is carried out at a relatively high temperature, dye loss may occur, but due to countercurrent contact with steam, there was almost no decrease in dye amount before and after treatment due to the short residence time at high temperatures.
【００３６】実施例１ 口紅 アスタキサンチン含有ファフィア酵母抽出油を色素とし
て用い、口紅を調製した。すなわち、表８に示す口紅基
剤を８０℃で加熱融解して均一に混入し、これに上記製
造例５で得られた濃縮脱臭ファフィア色素油を１％添
加、さらに混入して均一に分散した。脱泡したのち型に
流し込み、室温まで急冷して固めたものを型から取り出
し、炎の中を通して表面を均一にした。得られた口紅
は、非常に鮮明な橙色を有しており、４５℃で暗所に１
カ月放置しても異臭や変褪色は認められなかった。Example 1 Lipstick Lipstick was prepared using astaxanthin-containing Phaffia yeast extract oil as a pigment. That is, the lipstick bases shown in Table 8 were heated and melted at 80 ° C. and uniformly mixed, to which 1% of the concentrated deodorized Phaffia dye oil obtained in Production Example 5 was added, and further mixed and uniformly dispersed. . After defoaming, the mixture was poured into a mold, rapidly cooled to room temperature and solidified, taken out of the mold and passed through a flame to make the surface uniform. The lipstick obtained has a very bright orange color, and at 1 ° C in the dark at 45 ° C.
No strange odor or discoloration was observed even if left for months.
【００３７】[0037]
【表８】 [Table 8]
【００３８】[0038]
【発明の効果】天然色素に基づく日光、湿度、熱に強い
鮮やかな明るい赤色の化粧料を提供することができる。
天然色素の特徴を損なうことなく、これまで天然色素の
欠点となっていた着色性や安定性、色素濃度に優れた天
然赤色色素を配合した化粧料を提供することができる。
ファフィア酵母が生産する赤色色素アスタキサンチンを
高濃度で含有する夾雑物質の少ない、無臭の、溶剤を含
まない濃厚色素油を配合した、優れた赤色を有する化粧
料を提供することができる。EFFECTS OF THE INVENTION It is possible to provide a bright bright red cosmetic which is resistant to sunlight, humidity and heat and which is based on natural pigments.
It is possible to provide a cosmetic containing a natural red pigment excellent in coloring property, stability, and pigment concentration, which have been the drawbacks of natural pigments, without impairing the characteristics of the natural pigment.
It is possible to provide a cosmetic having an excellent red color, which is mixed with a concentrated pigment oil containing no odor and containing a high concentration of the red pigment astaxanthin produced by Phaffia yeast, which is odorless and solvent-free.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 (Ｃ１２Ｐ 1/02 Ｃ１２Ｒ 1:645） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification number Office reference number FI technical display location (C12P 1/02 C12R 1: 645)
Claims (2)
色素アスタキサンチンを高濃度で含有する濃縮ファフィ
ア色素油を配合してなることを特徴とする化粧料。1. A cosmetic composition comprising a concentrated Phaffia pigment oil containing a high concentration of natural red pigment astaxanthin which is excellent in color tone, light resistance and colorability.
属する赤色色素を生産する酵母から得られた濃縮天然赤
色色素油である請求項１記載の化粧料。2. The cosmetic according to claim 1, wherein the concentrated Phaffia pigment oil is a concentrated natural red pigment oil obtained from a yeast producing a red pigment belonging to the genus Phaffia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5277286A JPH07101827A (en) | 1993-09-30 | 1993-09-30 | Cosmetic containing concentrated phaffia pigment oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5277286A JPH07101827A (en) | 1993-09-30 | 1993-09-30 | Cosmetic containing concentrated phaffia pigment oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07101827A true JPH07101827A (en) | 1995-04-18 |
Family
ID=17581421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5277286A Withdrawn JPH07101827A (en) | 1993-09-30 | 1993-09-30 | Cosmetic containing concentrated phaffia pigment oil |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07101827A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013002398A1 (en) | 2011-06-30 | 2013-01-03 | 株式会社カネカ | Method for producing carotenoid composition |
| JP2018012675A (en) * | 2016-07-22 | 2018-01-25 | 国立大学法人広島大学 | Bacteria proliferation inhibitor |
| WO2023244565A1 (en) * | 2022-06-13 | 2023-12-21 | Adler Jesse | Solubilised pigments extracted from fungi for use in cosmetic products |
-
1993
- 1993-09-30 JP JP5277286A patent/JPH07101827A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013002398A1 (en) | 2011-06-30 | 2013-01-03 | 株式会社カネカ | Method for producing carotenoid composition |
| US9096508B2 (en) | 2011-06-30 | 2015-08-04 | Kaneka Corporation | Method for producing carotenoid composition |
| JP2018012675A (en) * | 2016-07-22 | 2018-01-25 | 国立大学法人広島大学 | Bacteria proliferation inhibitor |
| WO2023244565A1 (en) * | 2022-06-13 | 2023-12-21 | Adler Jesse | Solubilised pigments extracted from fungi for use in cosmetic products |
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|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20001226 |