JPH0549346A - Culture of mushroom - Google Patents
Culture of mushroomInfo
- Publication number
- JPH0549346A JPH0549346A JP3237289A JP23728991A JPH0549346A JP H0549346 A JPH0549346 A JP H0549346A JP 3237289 A JP3237289 A JP 3237289A JP 23728991 A JP23728991 A JP 23728991A JP H0549346 A JPH0549346 A JP H0549346A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- culture
- bottle
- mycelia
- filled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
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- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明はきのこ栽培方法、特に栽
培期間を大幅に短縮できるきのこ栽培方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cultivating mushrooms, and more particularly to a method for cultivating mushrooms which can significantly shorten the cultivation period.
【０００２】[0002]
【従来の技術】栽培瓶に培地を充填して、種菌の接種、
菌糸培養、子実体の生育を行うきのこの人工栽培が普及
している。この場合に栽培期間を短縮して、栽培瓶の回
転効率を高め、もって生産性を向上させる工夫が常に要
求される。2. Description of the Related Art A culture bottle is filled with a medium and inoculated with inoculum,
Artificial cultivation of mushrooms for hyphal culture and growth of fruiting bodies has become widespread. In this case, it is always required to shorten the cultivation period, improve the rotation efficiency of the cultivation bottle, and thus improve the productivity.
【０００３】[0003]
【発明が解決しようとする課題】例えば、ほんしめじ
（シロタモギタケ）の栽培期間は、菌糸の培養に約２５
日間、菌糸の熟成に約９０日間、子実体の生育に約１５
日間を要し、培養から収穫まで、１２０〜１４０日間の
長期間を要しているため、栽培瓶の回転が年間で２回転
位しかできず、生産性に劣る問題点がある。そこで本発
明は栽培期間、特に培養期間、熟成期間を大幅に短縮し
て生産性を高めることのできるきのこ栽培方法を提供す
ることを目的とする。[Problems to be Solved by the Invention] For example, the cultivation period of honshimeji mushrooms is about 25 for cultivation of hyphae.
Approximately 90 days for mycelial ripening and approximately 15 days for fruit body growth
Since it takes a long period of time from cultivation to harvesting for 120 to 140 days, the cultivation bottle can rotate only about two times a year, which causes a problem of poor productivity. Therefore, an object of the present invention is to provide a mushroom cultivating method capable of significantly shortening the culturing period, particularly the culturing period and the ripening period to enhance the productivity.
【０００４】[0004]
【課題を解決するための手段】本発明は上記目的を達成
するため次の構成を備える。すなわち、栽培瓶内に培地
を充填し、種菌の接種、培養、子実体の生育を行うきの
こ栽培方法において、オガ屑等の培地素材に必要な栄養
素を添加して殺菌処理をした培養基Ａと、オガ屑等を主
体とする培地素材に種菌を接種して培養して菌糸が充満
した培養基Ｂとを適宜比率で混合して培地Ｃを調整し、
この培地Ｃを栽培瓶に充填し、この培地Ｃ上にあらかじ
め培養が完了し、熟成期間中に入っている種菌Ｄを必要
量植菌し、培養を行うことを特徴としている。上記栽培
瓶は、培地Ｃが充填される部位の壁面に透孔が形成さ
れ、この透孔を覆って空気は流通させ、雑菌の流通を阻
止するフィルター部材を取り付けた栽培瓶を用いると好
適である。またさらに本発明では、栽培瓶内に培地を充
填し、種菌の接種、培養、子実体の生育を行うきのこ栽
培方法において、一度きのこを栽培し、野積みしておい
た古い培地素材に必要な栄養素を添加した培養基Ｅと、
オガ屑等を主体とする培地素材に種菌を接種して培養し
て菌糸が充満した培養基Ｂとを適宜比率で混合して培地
Ｆを調整し、この培地Ｆを栽培瓶に充填し、この培地Ｆ
上にあらかじめ培養が完了し、熟成期間中に入っている
種菌Ｄを必要量植菌し、培養を行うことを特徴としてい
る。The present invention has the following constitution in order to achieve the above object. That is, in a mushroom cultivation method in which a culture medium is filled in a culture bottle, inoculated with inoculum, cultivated, and fruit bodies are grown, a culture medium A sterilized by adding necessary nutrients to a medium material such as sawdust. Inoculate the medium material mainly composed of sawdust and inoculate it with the inoculum and mix it with the culture medium B filled with mycelia at an appropriate ratio to prepare the medium C,
This culture medium C is filled in a culture bottle, and the culture medium is preliminarily cultivated on the culture medium C, and a required amount of the inoculum D contained in the aging period is inoculated and cultured. It is preferable to use a cultivation bottle in which a through hole is formed in a wall surface of a portion where the culture medium C is filled, air is circulated to cover the through hole, and a filter member that prevents circulation of various bacteria is attached to the cultivation bottle. is there. Still further, in the present invention, in a mushroom cultivation method in which a culture bottle is filled with a medium, inoculation of inoculum, culture, and growth of fruiting bodies, mushrooms are cultivated once and necessary for an old medium material that has been piled up in the field. A culture medium E containing nutrients,
A culture medium B mainly containing sawdust is inoculated and cultured, and the culture medium B filled with mycelia is mixed at an appropriate ratio to prepare a culture medium F. The culture medium F is filled in a cultivation bottle, and the culture medium B is filled with the culture medium B. F
It is characterized in that the above-mentioned culture is completed in advance and the required amount of the inoculum D contained in the aging period is inoculated and the culture is performed.
【０００５】[0005]
【作用】上記のように培地Ｃ（Ｆ）を調整し、また種菌
Ｄを接種することによって、菌糸の培養期間および熟成
期間を大幅に短縮することができた。例えば、ほんしめ
じの場合、菌糸の培養期間は従来２５日程要したのが、
本発明方法によれば５日程に短縮され、また熟成期間は
従来９０日も要したのが、２５日程に短縮された。すな
わち、ほんしめじの場合、本発明方法によると、熟成終
了時まで３０日程度であり、この短期間経過後菌掻きを
行うことによって子実体の発生を見、以後従来と同様１
５日程の生育期間できのこの収穫が可能となった。した
がって、全栽培期間が４５日程であり、年間を通じて栽
培瓶の回転を６回程度行うことができ、生産性が非常に
向上した。By adjusting the medium C (F) and inoculating the inoculum D as described above, the culture period and ripening period of mycelia could be greatly shortened. For example, in the case of Hoshimeji, it took 25 days to culture mycelium.
According to the method of the present invention, it was shortened to about 5 days, and the aging period was conventionally 90 days, but it was shortened to about 25 days. That is, according to the method of the present invention, in the case of shimeji shimeji, it took about 30 days until the end of ripening, and after this short period of time, the generation of fruiting bodies was observed by scraping the bacteria.
It became possible to harvest this after a growing period of about 5 days. Therefore, the total cultivation period was about 45 days, the cultivation bottle could be rotated about 6 times throughout the year, and the productivity was greatly improved.
【０００６】[0006]
【実施例】以下、本発明の好適な実施例を添付図面に基
づいて詳細に説明する。図１は本発明に用いて好適な栽
培瓶１０を示す。栽培瓶１０は広口瓶形状をなし、その
側壁に複数の透孔１２が形成され、この透孔１２を覆っ
て、空気は流通させるが雑菌は通さないフィルター部材
１４が取り付けられてなる。フィルター部材１４の取付
は、図２に示すように、栽培瓶１０の側壁の上部および
下部に互いに対向する断面Ｌ字状の突周部１６を形成
し、この突周部１６の溝にフィルター部材１６の端部を
挿入するようにして突周部１６にてフィルター部材１６
を保持するようにするとよい。あるいはフィルター部材
の端縁部を接着剤にて栽培瓶外壁に固定して取り付ける
ようにしてもよい。さらに図３に示されるように、栽培
瓶１０の底面にも透孔１２を設け、この透孔１２を覆っ
て上記と同様にしてフィルター部材１４を取り付けるよ
うにしてもよい。フィルター部材１４の材質は、例えば
目の細かい不織布を用いることが出来る。あるいは、ミ
クロン単位の小孔のあいたプラスチックフィルムを用い
ることができる。フィルター部材１４は栽培瓶１０の内
側に透孔１２を覆って接着剤で固定するようにしてもよ
いが、フィルター部材１４の交換を容易にするため、上
記のように栽培瓶１０の外側に脱着自在に装着するのが
好適である。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT A preferred embodiment of the present invention will be described below in detail with reference to the accompanying drawings. FIG. 1 shows a cultivation bottle 10 suitable for use in the present invention. The cultivation bottle 10 has a wide-mouthed bottle shape, and a plurality of through holes 12 are formed in a side wall of the cultivation bottle 10, and a filter member 14 which covers the through holes 12 and allows air to flow but does not allow bacteria to pass therethrough is attached. As shown in FIG. 2, the attachment of the filter member 14 forms projecting peripheral portions 16 having L-shaped cross sections on the upper and lower portions of the side wall of the cultivation bottle 10, and the filter member is provided in the groove of the projecting peripheral portion 16. The filter member 16 is inserted in the projecting peripheral portion 16 so that the end portions of the filter member 16 are inserted.
Should be held. Alternatively, the edge portion of the filter member may be fixed and attached to the outer wall of the cultivation bottle with an adhesive. Further, as shown in FIG. 3, a through hole 12 may be provided in the bottom surface of the cultivation bottle 10, and the through hole 12 may be covered and the filter member 14 may be attached in the same manner as above. The material of the filter member 14 may be, for example, a fine-grained nonwoven fabric. Alternatively, a plastic film with micron-sized apertures can be used. The filter member 14 may cover the through hole 12 inside the cultivation bottle 10 and be fixed with an adhesive, but in order to facilitate the replacement of the filter member 14, the filter member 14 is detached from the cultivation bottle 10 as described above. It is suitable to be freely attached.
【０００７】続いて栽培方法について説明する。本発明
では、培地の調整方法に特徴がある。図４に示すよう
に、通常のごとくオガ屑等を主体とする培地素材にコヌ
カ等の必要な栄養素を添加し、水分調整をしたものを大
きな容器２０に入れ、蓋２１を被せた後殺菌釜に収納
し、常法により殺菌して培養基Ａを調整する。また図５
に示すように、オガ屑等を主体とする培地素材にコヌカ
等の必要な栄養素を添加し、水分調整をしたものを容器
２２に収納し、殺菌し、種菌を接種して菌糸培養を行っ
た培養基Ｂを調整する。この培養基Ｂは、菌糸が充満
し、培養が終了したもので、通常のきのこ栽培における
種菌としても用いうるものである。この培養基Ｂは、培
養基Ａの調整時にはもう既に調整が完了、すなわちほん
しめじの場合、既に２５日程経過して培養が完了してい
るものである。培養基Ｂの調整、すなわち培養はあらか
じめ別途行っておくのであり、したがって培養基Ｂ自体
の調整は栽培期間に算入されない。Next, a cultivation method will be described. The present invention is characterized by the method for adjusting the medium. As shown in FIG. 4, a medium material mainly composed of sawdust etc. is added with necessary nutrients such as Conuka as usual, and the water content is adjusted and put in a large container 20, and a lid 21 is put on the sterilization pot. Then, the culture medium A is prepared by sterilizing it by a conventional method. Also in FIG.
As shown in FIG. 5, necessary nutrients such as Conuka were added to a medium material mainly composed of sawdust and the water content was adjusted and stored in a container 22, sterilized, and inoculated with an inoculum to perform mycelial culture. Prepare culture medium B. The culture medium B is one that has been filled with hyphae and has been cultured, and can also be used as an inoculum in ordinary mushroom cultivation. The culture medium B has already been adjusted when the culture medium A was adjusted, that is, in the case of the shimeji mushroom, the culture was already completed after about 25 days. The adjustment of the culture medium B, that is, the culturing is performed separately in advance, and therefore the adjustment of the culture medium B itself is not included in the cultivation period.
【０００８】上記の培養基Ａと培養基Ｂとを適宜比率で
混合して培地Ｃを調整し、この培地Ｃを図６に示すよう
に上記した栽培瓶１０に常法により充填する。培地Ｃに
は空気流通孔を穿設する。培養基Ａと培養基Ｂの混合割
合はきのこの種類等によって異なるが、ほんしめじの場
合、２：１〜１：２程度の範囲が培養、熟成期間短縮の
上で好適であった。次に図７に示すように培地Ｃ上に種
菌Ｄを接種し、常法により菌糸の培養を行う。種菌Ｄ
は、培養が終了して既に熟成期間中に入ったもの、例え
ばほんしめじの場合培養開始時から４０日〜５０日程経
過したものを用いる。また図７に示すように、種菌Ｄの
量は通常のきのこ栽培時の量よりは多量に接種するのが
好適である。通常のきのこ栽培では種菌量は培地表面上
を覆う程度の量を接種するが、それよりもかなり多く接
種するのがよい。多量の種菌を接種するには、例えば図
８に示すように瓶口に所定厚さを有する孔明き板２４を
孔を瓶口に一致させて種菌Ｄを瓶口内および孔明き板２
４の孔内に収納されるように供給し、上から押圧板２５
で押圧して種菌Ｄを突き固め、種菌Ｄの表面位置が瓶口
縁から５ｍｍ程低い位置となるようにする。これによっ
て多量の種菌Ｄを接種できる。種菌Ｄ表面が瓶口縁より
も低くなるようにするのは、菌糸培養時に種菌表面上の
菌糸がキャップ内面に付着しないように間隔を確保する
ためである。The culture medium A and the culture medium B are mixed in an appropriate ratio to prepare a medium C, and the medium C is filled in the above-mentioned cultivation bottle 10 by a conventional method as shown in FIG. An air circulation hole is formed in the medium C. The mixing ratio of the culture medium A and the culture medium B varies depending on the type of mushroom, etc., but in the case of shimeji mushroom, a range of about 2: 1 to 1: 2 is suitable for shortening the culture and aging period. Next, as shown in FIG. 7, the inoculum D is inoculated on the medium C, and mycelia are cultured by a conventional method. Inoculum D
Is used after the culturing has been completed and has already entered the aging period, for example, in the case of Honshimeji, those which have been about 40 to 50 days after the start of the culture are used. Further, as shown in FIG. 7, it is preferable to inoculate a larger amount of the inoculum D than the amount during normal mushroom cultivation. In normal mushroom cultivation, the inoculum is inoculated so that it covers the surface of the medium, but it is preferable to inoculate much more than that. To inoculate a large amount of inoculum, for example, as shown in FIG. 8, a perforated plate 24 having a predetermined thickness is formed in the bottle mouth so that the holes coincide with the bottle mouth, and the inoculum D is placed in the bottle mouth and the perforated plate 2
4 is supplied so as to be housed in the hole of 4, and the pressing plate 25
Then, the seed germ D is pressed and solidified so that the surface position of the seed germ D is about 5 mm lower than the mouth edge of the bottle. With this, a large amount of inoculum D can be inoculated. The reason why the surface of the inoculum D is set to be lower than the rim of the bottle is to secure a space so that the hyphae on the surface of the inoculum do not adhere to the inner surface of the cap during mycelium culture.
【０００９】上記のように培地Ｃを調整し、また種菌Ｄ
を接種することによって、菌糸の培養期間および熟成期
間を大幅に短縮することができた。例えば、ほんしめじ
の場合、菌糸の培養期間は従来２５日程要したのが、上
記実施例では５日程に短縮され、また熟成期間は従来９
０日も要したのが、２５日程に短縮された。すなわち、
上記実施例では、熟成終了時まで３０日程度であり、こ
の短期間経過後種菌Ｄの表面を若干削り取る菌掻きをし
たところ子実体の発生を見、以後従来と同様１５日程の
生育期間できのこの収穫が可能となった。したがって、
全栽培期間が４５日程であり、年間を通じて栽培瓶の回
転を６回程度行うことができ、生産性が非常に向上し
た。The medium C was prepared as described above, and the inoculum D
It was possible to significantly shorten the culture period and ripening period of the mycelium by inoculating with. For example, in the case of Hon-shimeji, the culture period of hypha was conventionally about 25 days, but in the above example, it was shortened to about 5 days, and the aging period was 9 days.
It took 0 days, but it was shortened to 25 days. That is,
In the above-mentioned Example, it took about 30 days until the end of ripening, and after a short period of time, the surface of the inoculum D was scraped off slightly, and when the bacteria were scratched, the occurrence of fruiting bodies was observed. This harvest has become possible. Therefore,
The total cultivation period was about 45 days, and the cultivation bottle could be rotated about 6 times throughout the year, and the productivity was greatly improved.
【００１０】本実施例の場合、種菌Ｄ自体について注目
してみれば、接種時にすでに４０日〜５０日程経過した
熟成過程にあり、下方の培地Ｃの培養、熟成期間の合計
の３０日を合わせれば約７０日〜８０日経過したものと
なる。通常の場合、ほんしめじにあっては前記のごとく
約１１５日ほど経過しないと発芽しないが、本実施例の
場合、上記のように、下方の培地Ｃの培養、熟成が短期
間に進み、これが上方の種菌Ｄにも影響を与えて３０日
程で発芽可能となったものと考えられる。In the case of this example, paying attention to the inoculum D itself, it is in the aging process in which about 40 to 50 days have already passed at the time of inoculation, and the total of 30 days of the culture and aging period of the lower medium C should be combined. For example, about 70 to 80 days have passed. Normally, in the case of Honshimeji, germination does not occur until about 115 days have passed as described above, but in the case of this example, the culture and aging of the lower medium C proceeded in a short period of time as described above, and It is considered that the seed germ D above was also affected and germination was possible in about 30 days.
【００１１】培養期間、熟成期間が上記のように大幅に
短縮されたのは次の理由によると考えられる。まず１つ
には、培地Ｃが通常のきのこ栽培に用いられる培養基Ａ
と、既に菌糸が蔓延した培養基Ｂとを混合したものであ
るので、極めて菌糸回りがよいこと、次には、種菌Ｄが
既に培養が終了し、熟成期間に入ったものを大量に接種
することによって、下方の培地Ｃ内の菌糸の生育を促進
し、菌糸まわりを早めること、この相乗効果によって培
養期間、熟成期間が大幅に短縮される。培養基Ａと培養
基Ｂを混合することにより都合のよいことは、これらを
混合して調整する培地Ｃの量が変化しても培養、熟成期
間にほとんど変動がなく、短期間に行えることである。
これにより１瓶あたり多量の培地を充填しても栽培期間
に変わりはなく、したがって１瓶あたりの収量を多くす
ることができる。この点従来にあっては培地の量が多く
なればなる程、培地全体に菌糸が蔓延するのに時間を要
し、培養、熟成に長期間を要した。The reason why the culture period and the ripening period were greatly shortened as described above is considered to be as follows. First, the medium C is a culture medium A used for ordinary mushroom cultivation.
And the culture medium B in which the hyphae have already spread, so that the mycelium rotation is extremely good. Next, inoculate a large amount of the inoculum D that has already been cultured and has reached the aging period. Promotes the growth of mycelia in the lower medium C and accelerates the mycelial circumference, and the synergistic effect significantly shortens the culture period and ripening period. It is more convenient to mix the culture medium A and the culture medium B in that even if the amount of the medium C prepared by mixing them is changed, there is almost no change in the culturing and aging period, and the culture can be performed in a short period of time.
As a result, the cultivation period does not change even if a large amount of medium is filled per bottle, and therefore the yield per bottle can be increased. In this respect, in the conventional method, the larger the amount of the medium, the longer it took for the hyphae to spread throughout the medium, and the longer the culture and aging took.
【００１２】また、上記のように栽培瓶１０に、外壁に
透孔１２を設けたものを使用することにより、フィルタ
ー部材１４を介して該透孔１２から栽培瓶１０内に空気
が流通し、菌糸の生育が活発となって培養期間、熟成期
間が短縮されるのである。なお本発明においては、栽培
瓶１０は上記のものに限定されず、透孔のない従来の栽
培瓶を使用できる。この場合栽培瓶内への空気の流通は
キャップ（図示せず）に設けたフィルター部材を介して
のみ行われるので、空気流通による培養、熟成の促進は
上記よりも低くなるが、培地Ｃ、種菌Ｄを上記のように
調整することで培養期間、熟成期間はやはりかなり短縮
される。例えばほんしめじの場合従来の栽培瓶を用いた
としても培養期間は８日程となり、また熟成期間は３８
日程となってやはり従来に比して大幅な短縮となる。Further, by using the cultivation bottle 10 having the through hole 12 in the outer wall as described above, the air flows from the through hole 12 into the cultivation bottle 10 through the filter member 14, The growth of mycelium becomes active, and the culture period and ripening period are shortened. In the present invention, the cultivation bottle 10 is not limited to the above, and a conventional cultivation bottle having no through hole can be used. In this case, since the circulation of air into the cultivation bottle is performed only through the filter member provided in the cap (not shown), the culture by air circulation and the promotion of aging are lower than the above, but the medium C, the inoculum By adjusting D as described above, the culture period and ripening period can be considerably shortened. For example, in the case of honshi-shimeji, the culture period is about 8 days and the aging period is 38 even if a conventional culture bottle is used.
The schedule will be much shorter than before.
【００１３】上記実施例では、培養基Ａを全く新しい培
地素材のもので調整したが、既に１度きのこ栽培をした
古い培地を再利用できる。すなわち１年程野積みしてお
いた古い培地（表層部を除く）をそのまま使用し、これ
に不足しているコヌカ等の栄養素を添加し、水分調整し
て培養基Ｅを調整する。この培養基Ｅと前記培養基Ｂと
を前記と同様にして混合して培地Ｆを調整するのであ
る。この培地Ｆを用いて上記と同様に種菌Ｄを接種し、
菌糸の培養、熟成、子実体の生育を行ったところ、前記
と同様にして何ら支障なくきのこ栽培が行え、培養期
間、熟成期間も上記と同様短縮され、同等の収量を得る
ことができた。この実施例で都合のよいのは、古い培地
を再利用しうるというだけでなく、培養基Ｅの調整の際
殺菌処理が必要でないことである。殺菌処理が必要でな
い理由はさだかではないが、野積み期間中にきのこ菌の
生育を妨げず雑菌の繁殖を抑止する何らかのバクテリア
が繁殖するのではないかと考えられる。本発明はほんし
めじの栽培のみでなく、えのきたけ、その他人工栽培が
可能なきのこの栽培に汎用的に適用できる。In the above embodiment, the culture medium A was prepared with a completely new medium material, but an old medium which has already been cultivated once mushroom can be reused. That is, the old medium (excluding the surface layer portion) that has been piled up for about one year is used as it is, and nutrients such as deficient Conuka are added to this, and the water content is adjusted to adjust the culture medium E. The culture medium E and the culture medium B are mixed in the same manner as described above to prepare the medium F. Using this medium F, inoculate the inoculum D in the same manner as above,
When the mycelia were cultured, ripened, and fruiting bodies were grown, mushrooms could be cultivated without any trouble in the same manner as described above, and the culturing period and the aging period were shortened in the same manner as above, and an equivalent yield could be obtained. The advantage of this example is not only that the old medium can be reused, but that no disinfection treatment is necessary for the preparation of the culture medium E. The reason why sterilization is not necessary is not a big reason, but it is thought that some bacteria that do not prevent the growth of mushrooms and suppress the growth of various bacteria grow during the field-loading period. INDUSTRIAL APPLICABILITY The present invention can be applied not only to cultivating honshi-shimeji mushrooms, but also to cultivating mushrooms such as enoki mushrooms and other mushrooms that can be artificially cultivated.
【００１４】以上本発明につき好適な実施例を挙げて種
々説明したが、本発明はこの実施例に限定されるもので
はなく、発明の精神を逸脱しない範囲内で多くの改変を
施し得るのはもちろんである。Although the present invention has been variously described with reference to the preferred embodiments, the present invention is not limited to these embodiments, and many modifications can be made without departing from the spirit of the invention. Of course.
【００１５】[0015]
【発明の効果】本発明に係るきのこ栽培方法によれば、
上記したように培養期間、熟成期間の大幅な短縮が行
え、栽培期間を短縮して栽培瓶の効率的な回転使用がで
きるから、生産性を大きく向上させることができる。特
にフィルター部材で覆った透孔を有する栽培瓶を併用す
ることにより、瓶内への空気流通を良好に行え、培養、
熟成の一層の促進が図れ、栽培期間を短縮して大幅な生
産性の向上が図れる。培養基Ａ（Ｅ）と培養基Ｂを混合
することにより都合のよいことは、これらを混合して調
整する培地Ｃの量が変化しても培養、熟成期間にほとん
ど変動がなく、短期間に行えることである。これにより
１瓶あたり多量の培地を充填しても栽培期間に変わりは
なく、したがって１瓶あたりの収量を多くすることがで
きる。また培養基Ｅに１度きのこを栽培した古い培地を
再利用でき、この場合に殺菌処理が必要なく、工数の削
減が図れる。According to the mushroom cultivation method of the present invention,
As described above, the culturing period and the ripening period can be significantly shortened, and the cultivation period can be shortened to efficiently use the cultivation bottle for rotation, so that the productivity can be greatly improved. In particular, by using together with a cultivation bottle having a through hole covered with a filter member, it is possible to satisfactorily allow air to flow into the bottle, and culture,
Aging can be further promoted, the cultivation period can be shortened, and productivity can be greatly improved. It is convenient to mix the culture medium A (E) and the culture medium B so that even if the amount of the medium C prepared by mixing them is changed, there is almost no change in the culturing and aging period, and it can be performed in a short time. Is. As a result, the cultivation period does not change even if a large amount of medium is filled per bottle, and therefore the yield per bottle can be increased. In addition, an old medium in which mushrooms are once cultivated can be reused as the culture medium E, and in this case, sterilization treatment is not required and the number of steps can be reduced.
【図１】栽培瓶の正面図である。FIG. 1 is a front view of a cultivation bottle.
【図２】栽培瓶の部分断面図である。FIG. 2 is a partial cross-sectional view of a cultivation bottle.
【図３】栽培瓶の部分断面図である。FIG. 3 is a partial cross-sectional view of a cultivation bottle.
【図４】培養基Ａの調整を示す説明図である。FIG. 4 is an explanatory view showing the adjustment of culture medium A.
【図５】培養基Ｂの調整を示す説明図である。FIG. 5 is an explanatory view showing the adjustment of culture medium B.
【図６】培地を栽培瓶に充填した状態を示す断面図であ
る。FIG. 6 is a cross-sectional view showing a state in which a culture bottle is filled with a medium.
【図７】培地上に種菌を接種した状態を示す断面図であ
る。FIG. 7 is a cross-sectional view showing a state in which the inoculum is inoculated on the medium.
【図８】種菌の接種方法の一例を示す説明図である。FIG. 8 is an explanatory view showing an example of an inoculation method of inoculum.
１０ 栽培瓶 １２ 透孔 １４ フィルター部材 10 Cultivation Bottle 12 Through Hole 14 Filter Member
Claims (3)
培養、子実体の生育を行うきのこ栽培方法において、オ
ガ屑等の培地素材に必要な栄養素を添加して殺菌処理を
した培養基Ａと、オガ屑等を主体とする培地素材に種菌
を接種して培養して菌糸が充満した培養基Ｂとを適宜比
率で混合して培地Ｃを調整し、この培地Ｃを栽培瓶に充
填し、この培地Ｃ上にあらかじめ培養が完了し、熟成期
間中に入っている種菌Ｄを必要量植菌し、培養を行うこ
とを特徴とするきのこ栽培方法。1. A culture bottle is filled with a medium and inoculated with an inoculum,
In a mushroom cultivation method for culturing and growing fruiting bodies, a culture medium A in which necessary nutrients are added to a medium material such as sawdust and sterilized, and a culture medium mainly containing the sawdust is inoculated with an inoculum. The medium C is cultivated and mixed with the culture medium B filled with mycelia at an appropriate ratio to prepare the medium C, and the medium C is filled in a cultivation bottle. The culture is completed on the medium C in advance and the aging period is started. A method for cultivating mushrooms, which comprises inoculating a required amount of inoculum D present and culturing.
形成され、この透孔を覆って空気は流通させ、雑菌の流
通を阻止するフィルター部材を取り付けた栽培瓶を用い
ることを特徴とする請求項１記載のきのこ栽培方法。2. A culture bottle is provided with a through hole formed in a wall surface of a portion to be filled with the medium C, and a culture bottle attached with a filter member which covers the through hole to allow air to flow therethrough and prevent the passage of miscellaneous bacteria. The mushroom cultivation method according to claim 1.
培養、子実体の生育を行うきのこ栽培方法において、一
度きのこを栽培し、野積みしておいた古い培地素材に必
要な栄養素を添加した培養基Ｅと、オガ屑等を主体とす
る培地素材に種菌を接種して培養して菌糸が充満した培
養基Ｂとを適宜比率で混合して培地Ｆを調整し、この培
地Ｆを栽培瓶に充填し、この培地Ｆ上にあらかじめ培養
が完了し、熟成期間中に入っている種菌Ｄを必要量植菌
し、培養を行うことを特徴とするきのこ栽培方法。3. A culture bottle is filled with a medium and inoculated with an inoculum,
In a mushroom cultivating method for culturing and growing fruiting bodies, a culturing medium E in which mushrooms are cultivated once and the nutrients necessary for the old medium material that has been accumulated in the field are added, and a medium material mainly composed of sawdust etc. Inoculate and culture and mix with the culture medium B filled with hypha at an appropriate ratio to prepare the medium F, fill the medium F in a culture bottle, and complete the culture on the medium F in advance, and the aging period A method for cultivating mushrooms, which comprises inoculating a required amount of inoculum D contained therein and culturing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3237289A JPH0549346A (en) | 1991-08-23 | 1991-08-23 | Culture of mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3237289A JPH0549346A (en) | 1991-08-23 | 1991-08-23 | Culture of mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0549346A true JPH0549346A (en) | 1993-03-02 |
Family
ID=17013171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3237289A Pending JPH0549346A (en) | 1991-08-23 | 1991-08-23 | Culture of mushroom |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0549346A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007061089A (en) * | 2005-08-05 | 2007-03-15 | Hokken Co Ltd | Method for growing shiitake mushroom bed |
| JP2007082539A (en) * | 2005-08-22 | 2007-04-05 | Hokken Co Ltd | Method for culturing mushroom bed of lentinus edodes |
| JP2007209303A (en) * | 2006-02-13 | 2007-08-23 | Nakanoshi Nogyo Kyodo Kumiai | Cultivation method of pleurotus nebrodensis |
| CN102550293A (en) * | 2012-02-03 | 2012-07-11 | 连云港市农业科学院 | Method for liquid fermentation cultivation of Agaricus bisporus strain |
| CN102550294A (en) * | 2012-02-03 | 2012-07-11 | 连云港市农业科学院 | Method for liquid fermentation cultivation of Pleurotus cornucopiae strain |
| CN103004453A (en) * | 2011-09-20 | 2013-04-03 | 杭州海智生物技术有限公司 | Manufacturing method of edible fungi cultivar and culture medium manufacturing raw materials for edible fungi cultivar |
| CN103749151A (en) * | 2013-12-26 | 2014-04-30 | 广东省微生物研究所 | Artificial cultivation method of Amauroderma rude |
-
1991
- 1991-08-23 JP JP3237289A patent/JPH0549346A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007061089A (en) * | 2005-08-05 | 2007-03-15 | Hokken Co Ltd | Method for growing shiitake mushroom bed |
| JP2007082539A (en) * | 2005-08-22 | 2007-04-05 | Hokken Co Ltd | Method for culturing mushroom bed of lentinus edodes |
| JP4647563B2 (en) * | 2005-08-22 | 2011-03-09 | 株式会社北研 | Shiitake fungus cultivation method |
| JP2007209303A (en) * | 2006-02-13 | 2007-08-23 | Nakanoshi Nogyo Kyodo Kumiai | Cultivation method of pleurotus nebrodensis |
| CN103004453A (en) * | 2011-09-20 | 2013-04-03 | 杭州海智生物技术有限公司 | Manufacturing method of edible fungi cultivar and culture medium manufacturing raw materials for edible fungi cultivar |
| CN102550293A (en) * | 2012-02-03 | 2012-07-11 | 连云港市农业科学院 | Method for liquid fermentation cultivation of Agaricus bisporus strain |
| CN102550294A (en) * | 2012-02-03 | 2012-07-11 | 连云港市农业科学院 | Method for liquid fermentation cultivation of Pleurotus cornucopiae strain |
| CN103749151A (en) * | 2013-12-26 | 2014-04-30 | 广东省微生物研究所 | Artificial cultivation method of Amauroderma rude |
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