JPH0463680B2 - - Google Patents

Info

Publication number: JPH0463680B2
Authority: JP; Japan
Prior art keywords: dna; fragments; core; sequence; probe
Prior art date: 1984-11-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP60504605A

Other languages

English (en)

Japanese (ja)

Other versions

JPS62501467A (ja

Inventor

Ariku Jon Jefuriizu

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

RISUTAA INST OBU PURIBENTEIBU MEDEISUN

Original Assignee

RISUTAA INST OBU PURIBENTEIBU MEDEISUN

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-11-12

Filing date

1985-10-17

Publication date

1992-10-12

1985-10-17 Application filed by RISUTAA INST OBU PURIBENTEIBU MEDEISUN filed Critical RISUTAA INST OBU PURIBENTEIBU MEDEISUN

1987-06-18 Publication of JPS62501467A publication Critical patent/JPS62501467A/ja

1992-10-12 Publication of JPH0463680B2 publication Critical patent/JPH0463680B2/ja

Status Granted legal-status Critical Current

Links

108020004414 DNA Proteins 0.000 description 337
239000012634 fragment Substances 0.000 description 233
239000000523 sample Substances 0.000 description 231
108091092919 Minisatellite Proteins 0.000 description 93
241000282414 Homo sapiens Species 0.000 description 77
238000000034 method Methods 0.000 description 71
239000002773 nucleotide Substances 0.000 description 57
125000003729 nucleotide group Chemical group 0.000 description 52
108091033319 polynucleotide Proteins 0.000 description 40
102000040430 polynucleotide Human genes 0.000 description 40
239000002157 polynucleotide Substances 0.000 description 40
108700028369 Alleles Proteins 0.000 description 36
238000004458 analytical method Methods 0.000 description 36
230000008775 paternal effect Effects 0.000 description 33
230000008774 maternal effect Effects 0.000 description 31
210000004369 blood Anatomy 0.000 description 30
239000008280 blood Substances 0.000 description 30
238000009396 hybridization Methods 0.000 description 30
241001465754 Metazoa Species 0.000 description 26
241000283690 Bos taurus Species 0.000 description 22
230000002068 genetic effect Effects 0.000 description 22
238000012360 testing method Methods 0.000 description 22
239000002585 base Substances 0.000 description 20
210000000582 semen Anatomy 0.000 description 18
108090000623 proteins and genes Proteins 0.000 description 17
238000000926 separation method Methods 0.000 description 17
208000009905 Neurofibromatoses Diseases 0.000 description 16
239000011543 agarose gel Substances 0.000 description 16
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 16
108010062374 Myoglobin Proteins 0.000 description 15
238000001962 electrophoresis Methods 0.000 description 15
201000004931 neurofibromatosis Diseases 0.000 description 15
108091008146 restriction endonucleases Proteins 0.000 description 15
201000010099 disease Diseases 0.000 description 14
230000000295 complement effect Effects 0.000 description 13
210000002826 placenta Anatomy 0.000 description 13
241000282412 Homo Species 0.000 description 12
210000004027 cell Anatomy 0.000 description 12
238000003776 cleavage reaction Methods 0.000 description 12
239000000499 gel Substances 0.000 description 12
230000007017 scission Effects 0.000 description 11
239000003298 DNA probe Substances 0.000 description 10
238000007894 restriction fragment length polymorphism technique Methods 0.000 description 10
231100000241 scar Toxicity 0.000 description 10
238000000211 autoradiogram Methods 0.000 description 9
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 8
108091081062 Repeated sequence (DNA) Proteins 0.000 description 8
230000029087 digestion Effects 0.000 description 8
239000003550 marker Substances 0.000 description 8
241000282472 Canis lupus familiaris Species 0.000 description 7
102100030856 Myoglobin Human genes 0.000 description 7
241001494479 Pecora Species 0.000 description 7
238000010367 cloning Methods 0.000 description 7
238000004519 manufacturing process Methods 0.000 description 7
108091035707 Consensus sequence Proteins 0.000 description 6
102000053602 DNA Human genes 0.000 description 6
241000588724 Escherichia coli Species 0.000 description 6
239000000020 Nitrocellulose Substances 0.000 description 6
239000000306 component Substances 0.000 description 6
238000009826 distribution Methods 0.000 description 6
239000004744 fabric Substances 0.000 description 6
229920001220 nitrocellulos Polymers 0.000 description 6
210000004940 nucleus Anatomy 0.000 description 6
229920000642 polymer Polymers 0.000 description 6
208000032544 Cicatrix Diseases 0.000 description 5
241000287828 Gallus gallus Species 0.000 description 5
108020004487 Satellite DNA Proteins 0.000 description 5
108020004682 Single-Stranded DNA Proteins 0.000 description 5
244000309466 calf Species 0.000 description 5
235000013330 chicken meat Nutrition 0.000 description 5
238000010586 diagram Methods 0.000 description 5
230000000694 effects Effects 0.000 description 5
238000012252 genetic analysis Methods 0.000 description 5
230000037387 scars Effects 0.000 description 5
238000012163 sequencing technique Methods 0.000 description 5
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
241000283086 Equidae Species 0.000 description 4
241000282326 Felis catus Species 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
241000699666 Mus <mouse, genus> Species 0.000 description 4
241000699670 Mus sp. Species 0.000 description 4
206010028980 Neoplasm Diseases 0.000 description 4
108091034117 Oligonucleotide Proteins 0.000 description 4
241000283973 Oryctolagus cuniculus Species 0.000 description 4
241000282887 Suidae Species 0.000 description 4
241000251539 Vertebrata <Metazoa> Species 0.000 description 4
238000003491 array Methods 0.000 description 4
230000005540 biological transmission Effects 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
244000309464 bull Species 0.000 description 4
210000000349 chromosome Anatomy 0.000 description 4
238000010276 construction Methods 0.000 description 4
238000003745 diagnosis Methods 0.000 description 4
210000004209 hair Anatomy 0.000 description 4
238000003780 insertion Methods 0.000 description 4
230000037431 insertion Effects 0.000 description 4
238000005259 measurement Methods 0.000 description 4
239000000203 mixture Substances 0.000 description 4
239000000178 monomer Substances 0.000 description 4
230000003169 placental effect Effects 0.000 description 4
102000054765 polymorphisms of proteins Human genes 0.000 description 4
238000011160 research Methods 0.000 description 4
238000005204 segregation Methods 0.000 description 4
ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 4
241000251468 Actinopterygii Species 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
108091005904 Hemoglobin subunit beta Proteins 0.000 description 3
208000026350 Inborn Genetic disease Diseases 0.000 description 3
241000699660 Mus musculus Species 0.000 description 3
108091028043 Nucleic acid sequence Proteins 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
238000002105 Southern blotting Methods 0.000 description 3
208000007502 anemia Diseases 0.000 description 3
238000009395 breeding Methods 0.000 description 3
230000001488 breeding effect Effects 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
239000008358 core component Substances 0.000 description 3
230000009089 cytolysis Effects 0.000 description 3
230000007423 decrease Effects 0.000 description 3
239000003814 drug Substances 0.000 description 3
238000012869 ethanol precipitation Methods 0.000 description 3
238000000605 extraction Methods 0.000 description 3
208000016361 genetic disease Diseases 0.000 description 3
238000002955 isolation Methods 0.000 description 3
239000000463 material Substances 0.000 description 3
239000013612 plasmid Substances 0.000 description 3
229920001184 polypeptide Polymers 0.000 description 3
108090000765 processed proteins & peptides Proteins 0.000 description 3
102000004196 processed proteins & peptides Human genes 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
230000006798 recombination Effects 0.000 description 3
238000005215 recombination Methods 0.000 description 3
241000894007 species Species 0.000 description 3
238000010561 standard procedure Methods 0.000 description 3
238000006467 substitution reaction Methods 0.000 description 3
208000035203 thalassemia minor Diseases 0.000 description 3
235000021419 vinegar Nutrition 0.000 description 3
239000000052 vinegar Substances 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 2
OLXZPDWKRNYJJZ-RRKCRQDMSA-N 2'-deoxyadenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 OLXZPDWKRNYJJZ-RRKCRQDMSA-N 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
241000271566 Aves Species 0.000 description 2
UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 2
238000007400 DNA extraction Methods 0.000 description 2
238000007399 DNA isolation Methods 0.000 description 2
108010067770 Endopeptidase K Proteins 0.000 description 2
101100481527 Escherichia phage N15 gene 19 gene Proteins 0.000 description 2
YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical compound C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 2
NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
208000034702 Multiple pregnancies Diseases 0.000 description 2
241000287462 Phalacrocorax carbo Species 0.000 description 2
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
210000001766 X chromosome Anatomy 0.000 description 2
210000002593 Y chromosome Anatomy 0.000 description 2
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
238000000376 autoradiography Methods 0.000 description 2
230000037429 base substitution Effects 0.000 description 2
230000027455 binding Effects 0.000 description 2
201000011510 cancer Diseases 0.000 description 2
239000013068 control sample Substances 0.000 description 2
UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 2
229960000633 dextran sulfate Drugs 0.000 description 2
239000005546 dideoxynucleotide Substances 0.000 description 2
208000035475 disorder Diseases 0.000 description 2
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
229960005542 ethidium bromide Drugs 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
210000004700 fetal blood Anatomy 0.000 description 2
238000001502 gel electrophoresis Methods 0.000 description 2
102000054766 genetic haplotypes Human genes 0.000 description 2
238000011065 in-situ storage Methods 0.000 description 2
238000007689 inspection Methods 0.000 description 2
210000000265 leukocyte Anatomy 0.000 description 2
230000033001 locomotion Effects 0.000 description 2
210000004698 lymphocyte Anatomy 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
230000035772 mutation Effects 0.000 description 2
230000036961 partial effect Effects 0.000 description 2
210000005259 peripheral blood Anatomy 0.000 description 2
239000011886 peripheral blood Substances 0.000 description 2
230000002688 persistence Effects 0.000 description 2
210000005152 placental membrane Anatomy 0.000 description 2
230000002829 reductive effect Effects 0.000 description 2
230000008439 repair process Effects 0.000 description 2
230000002441 reversible effect Effects 0.000 description 2
238000012216 screening Methods 0.000 description 2
239000001632 sodium acetate Substances 0.000 description 2
235000017281 sodium acetate Nutrition 0.000 description 2
239000001509 sodium citrate Substances 0.000 description 2
238000001228 spectrum Methods 0.000 description 2
229940063673 spermidine Drugs 0.000 description 2
210000002784 stomach Anatomy 0.000 description 2
238000003786 synthesis reaction Methods 0.000 description 2
239000013638 trimer Substances 0.000 description 2
PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 2
101710190981 50S ribosomal protein L6 Proteins 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
241000269350 Anura Species 0.000 description 1
208000031872 Body Remains Diseases 0.000 description 1
208000005623 Carcinogenesis Diseases 0.000 description 1
241000777300 Congiopodidae Species 0.000 description 1
241000699800 Cricetinae Species 0.000 description 1
MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
102000012410 DNA Ligases Human genes 0.000 description 1
108010061982 DNA Ligases Proteins 0.000 description 1
102000004594 DNA Polymerase I Human genes 0.000 description 1
108010017826 DNA Polymerase I Proteins 0.000 description 1
108020003215 DNA Probes Proteins 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
108700003861 Dominant Genes Proteins 0.000 description 1
241000257465 Echinoidea Species 0.000 description 1
102100031780 Endonuclease Human genes 0.000 description 1
108010042407 Endonucleases Proteins 0.000 description 1
108010044495 Fetal Hemoglobin Proteins 0.000 description 1
102100021519 Hemoglobin subunit beta Human genes 0.000 description 1
108091005905 Hemoglobin subunit zeta Proteins 0.000 description 1
108010054147 Hemoglobins Proteins 0.000 description 1
102000001554 Hemoglobins Human genes 0.000 description 1
101000976075 Homo sapiens Insulin Proteins 0.000 description 1
101000635854 Homo sapiens Myoglobin Proteins 0.000 description 1
241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
108091007491 NSP3 Papain-like protease domains Proteins 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
241000287127 Passeridae Species 0.000 description 1
108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
201000000582 Retinoblastoma Diseases 0.000 description 1
108091006629 SLC13A2 Proteins 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
208000024780 Urticaria Diseases 0.000 description 1
241000700605 Viruses Species 0.000 description 1
241000269457 Xenopus tropicalis Species 0.000 description 1
239000002253 acid Substances 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
239000003513 alkali Substances 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
230000008485 antagonism Effects 0.000 description 1
239000000427 antigen Substances 0.000 description 1
230000000890 antigenic effect Effects 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
238000013459 approach Methods 0.000 description 1
238000012550 audit Methods 0.000 description 1
230000006399 behavior Effects 0.000 description 1
230000008901 benefit Effects 0.000 description 1
DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
230000037396 body weight Effects 0.000 description 1
238000010322 bone marrow transplantation Methods 0.000 description 1
UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
239000000872 buffer Substances 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
230000036952 cancer formation Effects 0.000 description 1
231100000504 carcinogenesis Toxicity 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
210000003855 cell nucleus Anatomy 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
210000003169 central nervous system Anatomy 0.000 description 1
230000008859 change Effects 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
210000000078 claw Anatomy 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
108091036078 conserved sequence Proteins 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
229920001577 copolymer Polymers 0.000 description 1
238000012937 correction Methods 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
210000004748 cultured cell Anatomy 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
238000007405 data analysis Methods 0.000 description 1
230000034994 death Effects 0.000 description 1
230000007547 defect Effects 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
238000013461 design Methods 0.000 description 1
230000001066 destructive effect Effects 0.000 description 1
239000000539 dimer Substances 0.000 description 1
238000002224 dissection Methods 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
210000002919 epithelial cell Anatomy 0.000 description 1
210000003743 erythrocyte Anatomy 0.000 description 1
DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
239000002871 fertility agent Substances 0.000 description 1
238000013467 fragmentation Methods 0.000 description 1
238000006062 fragmentation reaction Methods 0.000 description 1
108091008053 gene clusters Proteins 0.000 description 1
230000014509 gene expression Effects 0.000 description 1
239000011521 glass Substances 0.000 description 1
229940029575 guanosine Drugs 0.000 description 1
238000009399 inbreeding Methods 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
239000003999 initiator Substances 0.000 description 1
239000000543 intermediate Substances 0.000 description 1
238000002372 labelling Methods 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
238000009630 liquid culture Methods 0.000 description 1
210000004185 liver Anatomy 0.000 description 1
244000144972 livestock Species 0.000 description 1
238000011068 loading method Methods 0.000 description 1
230000004807 localization Effects 0.000 description 1
230000007774 longterm Effects 0.000 description 1
238000013507 mapping Methods 0.000 description 1
239000011159 matrix material Substances 0.000 description 1
238000000691 measurement method Methods 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
230000000394 mitotic effect Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
125000000449 nitro group Chemical class [O-][N+](*)=O 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
125000001820 oxy group Chemical group [*:1]O[*:2] 0.000 description 1
239000008188 pellet Substances 0.000 description 1
230000002093 peripheral effect Effects 0.000 description 1
210000001428 peripheral nervous system Anatomy 0.000 description 1
239000002504 physiological saline solution Substances 0.000 description 1
238000003976 plant breeding Methods 0.000 description 1
229940093429 polyethylene glycol 6000 Drugs 0.000 description 1
239000011148 porous material Substances 0.000 description 1
238000011533 pre-incubation Methods 0.000 description 1
239000002243 precursor Substances 0.000 description 1
230000035935 pregnancy Effects 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
230000008569 process Effects 0.000 description 1
238000003908 quality control method Methods 0.000 description 1
238000011002 quantification Methods 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
229920005604 random copolymer Polymers 0.000 description 1
230000009467 reduction Effects 0.000 description 1
108091035233 repetitive DNA sequence Proteins 0.000 description 1
102000053632 repetitive DNA sequence Human genes 0.000 description 1
230000003252 repetitive effect Effects 0.000 description 1
230000010076 replication Effects 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
239000007787 solid Substances 0.000 description 1
230000000392 somatic effect Effects 0.000 description 1
230000009870 specific binding Effects 0.000 description 1
238000012546 transfer Methods 0.000 description 1
230000009466 transformation Effects 0.000 description 1
238000013519 translation Methods 0.000 description 1
230000017105 transposition Effects 0.000 description 1
HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
229940038773 trisodium citrate Drugs 0.000 description 1
210000004881 tumor cell Anatomy 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
229940045145 uridine Drugs 0.000 description 1
238000005406 washing Methods 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

JP50460585A 1984-11-12 1985-10-17 ポリヌクレオチドプロ−ブ Granted JPS62501467A (ja)

Applications Claiming Priority (5)

Application Number	Priority Date	Filing Date	Title
GB848428491A GB8428491D0 (en)	1984-11-12	1984-11-12	Polynucleotide probes
GB8428491		1984-11-12
GB8505744		1985-03-06
GB8518755		1985-07-24
GB8522135		1985-09-06

Related Child Applications (1)

Application Number	Title	Priority Date	Filing Date
JP3149288A Division JP2750228B2 (ja)	1984-11-12	1991-05-27	遺伝的特性決定用ポリヌクレオチド

Publications (2)

Publication Number	Publication Date
JPS62501467A JPS62501467A (ja)	1987-06-18
JPH0463680B2 true JPH0463680B2 (de)	1992-10-12

Family

ID=10569583

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP50460585A Granted JPS62501467A (ja)	1984-11-12	1985-10-17	ポリヌクレオチドプロ−ブ

Country Status (3)

Country	Link
JP (1)	JPS62501467A (de)
GB (1)	GB8428491D0 (de)
ZA (1)	ZA858045B (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN105506035A (zh) *	2016-02-05	2016-04-20	广州复能基因有限公司	以多重dna片段为模板制备rna或dna探针的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS59199000A (ja) *	1983-02-28	1984-11-10	ライフコーズ・コーポレイション	父系の決定および個々の遺伝的同一性を確立する方法

1984
- 1984-11-12 GB GB848428491A patent/GB8428491D0/en active Pending
1985
- 1985-10-17 JP JP50460585A patent/JPS62501467A/ja active Granted
- 1985-10-18 ZA ZA858045A patent/ZA858045B/xx unknown

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS59199000A (ja) *	1983-02-28	1984-11-10	ライフコーズ・コーポレイション	父系の決定および個々の遺伝的同一性を確立する方法

Also Published As

Publication number	Publication date
ZA858045B (en)	1987-06-24
GB8428491D0 (en)	1984-12-19
JPS62501467A (ja)	1987-06-18

Publication	Publication Date	Title
JP2750228B2 (ja)	1998-05-13	遺伝的特性決定用ポリヌクレオチド
Marwal et al.	2020	Molecular markers: tool for genetic analysis
Jones et al.	1997	Microsatellite analysis of maternity and the mating system in the Gulf pipefish Syngnathus scovelli, a species with male pregnancy and sex‐role reversal
Schmid et al.	2005	Second report on chicken genes and chromosomes 2005
US5994075A (en)	1999-11-30	Methods for identifying a mutation in a gene of interest without a phenotypic guide
EP0238329B1 (de)	1994-04-27	Genetische Sonden
JP5176248B2 (ja)	2013-04-03	鶏卵の卵殻強度に関わる遺伝子変異の検出方法
Allendorf et al.	1994	Sex-linkage of two enzyme loci in Oncorhyncus mykiss (rainbow trout)
AU2009275988B2 (en)	2014-03-27	A genetic marker test for Brachyspina and fertility in cattle
Skuder et al.	1995	A polymorphism in mitochondrial DNA associated with IQ?
JPH11507548A (ja)	1999-07-06	ブタのリッターサイズ用ｄｎａマーカー
US6015670A (en)	2000-01-18	Methods for identifying a mutation in a gene of interest without a phenotypic guide using ES cells
JP2719337B2 (ja)	1998-02-25	Ｙ‐染色体特異的ポリヌクレオチドを用いた反芻動物の性別判定
EP0342717A2 (de)	1989-11-23	Polynukleotidproben
CA2071105A1 (en)	1992-12-14	Nucleotide sequences
JPH0463680B2 (de)	1992-10-12
JP2004529649A (ja)	2004-09-30	方法
Le et al.	1990	Analysis of formaldehyde-induced Adh mutations in Drosophila by RNA structure mapping and direct sequencing of PCR-amplified genomic DNA
JP4776037B2 (ja)	2011-09-21	遺伝情報によりブタ筋肉中脂肪蓄積能力を評価する方法
Van Haeringen	1998	DNA analysis in paternity testing of dogs and cats
US8431346B2 (en)	2013-04-30	Screening for arthrogryposis multiplex in bovines
Bhebhe	1993	Isolation of molecular genetic markers and a search for possible associations with parasite resistance and quantitative trait loci in Capra hircus
WO2011075144A1 (en)	2011-06-23	Screening for arthrogryposis multiplex in bovines
Hansen	2003	Evaluating six positional candidate genes for effects on meat tenderness
Schmulian	2005	Identification of the polled trait in Bonsmara cattle using microsatellite markers