JP7393043B2 - オニノヤガラの有機栽培方法 - Google Patents
オニノヤガラの有機栽培方法 Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
- A01G22/63—Orchids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Description
前記成長促進液及び前記接種基質は、新鮮なオニノヤガラの茎を細断して処理対象材料を得、バチルス・プミルス菌液を前記処理対象材料にスプレーし、発酵・培養して発酵後の材料を得、溶媒としてエタノールを使用して発酵後の材料に対して熱浸漬抽出を行い、次に固液分離して残渣及び抽出液を得、前記残渣を湿熱滅菌、乾燥処理及び粉砕して前記接種基質を得、前記抽出液を濃縮、再溶解及び湿熱滅菌して成長促進液を得ることによって調製される。
Claims (10)
- オニノヤガラの有機栽培方法であって、クヌギの枝を棒材に切断し、棒材に魚鱗様の開口である魚鱗口を切り取って魚鱗口付きの棒材を得、次に沸騰水及び成長促進液で魚鱗口付きの棒材を順に浸漬し、成長促進処理後の棒材を得、次に成長促進処理後の棒材の各魚鱗口にナラタケ種及び接種基質を入れて菌材を得るという菌材の調製ステップを含み、
前記成長促進液及び前記接種基質は、新鮮なオニノヤガラの茎を細断して処理対象材料を得、バチルス・プミルス菌液を前記処理対象材料にスプレーし、発酵・培養して発酵後の材料を得、エタノールを溶媒として発酵後の材料に対して熱浸漬抽出を行い、次に固液分離して残渣及び抽出液を得、前記残渣を湿熱滅菌、乾燥処理及び粉砕して前記接種基質を得、前記抽出液を濃縮、再溶解及び湿熱滅菌して成長促進液を得ることによって調製されることを特徴とするオニノヤガラの有機栽培方法。 - 前記発酵培養の温度が40~45℃であり、回転数が100rpmであり、時間が48hであり、発酵培養過程で、2~3hごとに前記処理対象材料にバチルス・プミルス菌液をスプレーし、毎回スプレーする時、処理対象材料とバチルス・プミルス菌液の使用量比は100g:15~20mlであることを特徴とする請求項1に記載のオニノヤガラの有機栽培方法。
- バチルス・プミルス菌液のOD600値が1であることを特徴とする請求項2に記載のオニノヤガラの有機栽培方法。
- 前記熱浸漬抽出の温度が80~90℃であり、時間が10~12hであることを特徴とする請求項3に記載のオニノヤガラの有機栽培方法。
- 熱浸漬抽出において、前記エタノールの体積百分率が80%であり、発酵後の材料とエタノールの使用量比が1g:25mLであることを特徴とする請求項4に記載のオニノヤガラの有機栽培方法。
- 前記抽出液を相対密度1.10のエキスに濃縮し、次にエキス1g当たり25mLの水で再溶解し、さらに湿熱滅菌して前記成長促進液を得ることを特徴とする請求項5に記載のオニノヤガラの有機栽培方法。
- 前記残渣を湿熱滅菌した後、50℃の条件下で含水量が5%未満になるまで乾燥させ、さらに粉砕して1番篩にかけて前記接種基質を得ることを特徴とする請求項6に記載のオニノヤガラの有機栽培方法。
- ナラタケ菌種と接種基質の質量比が2~4:1であることを特徴とする請求項7に記載のオニノヤガラの有機栽培方法。
- 沸騰水及び成長促進液で魚鱗口付き棒材を順に浸漬する方法は、沸騰水で棒材を5min浸漬し、棒材が冷却した後、成長促進液で棒材を12h浸漬することであることを特徴とする請求項8に記載のオニノヤガラの有機栽培方法。
- 前記棒材の直径が8~10cmであり、長さが60~70cmであり、前記魚鱗口は棒材の長さ方向に沿って配列され、隣接する魚鱗口は2~3cm離れていることを特徴とする請求項9に記載のオニノヤガラの有機栽培方法。
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CN202111660318.9A CN114208616B (zh) | 2021-12-31 | 2021-12-31 | 一种天麻的有机种植方法 |
CN202111660318.9 | 2021-12-31 |
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CN115517142A (zh) * | 2022-08-23 | 2022-12-27 | 西南林业大学 | 一种天麻的替代种植方法 |
CN115336503B (zh) * | 2022-08-30 | 2024-01-16 | 西南林业大学 | 一种天麻栽培用替代菌材的制备方法 |
CN118252898B (zh) * | 2024-05-30 | 2024-08-09 | 四川好培养生物工程有限公司 | 一种天麻提取物及其制备工艺、培养基 |
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