JP6347949B2 - 改良型細胞培養培地 - Google Patents
改良型細胞培養培地 Download PDFInfo
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- JP6347949B2 JP6347949B2 JP2013506612A JP2013506612A JP6347949B2 JP 6347949 B2 JP6347949 B2 JP 6347949B2 JP 2013506612 A JP2013506612 A JP 2013506612A JP 2013506612 A JP2013506612 A JP 2013506612A JP 6347949 B2 JP6347949 B2 JP 6347949B2
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
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Description
Claims (10)
- 組換えポリペプチドを産生するための方法であって、
10対1と1対1の間のナトリウムイオン対カリウムイオンのモル比によって特徴付けられ、ナトリウムイオンの濃度が50mMと90mMの間である、無血清および無タンパク質の細胞培養培地中で哺乳動物細胞を培養すること、および
組換えポリペプチドを発現させることを含む方法。 - ナトリウムイオン対カリウムイオンのモル比が8対1と6対1の間である、請求項1に記載の方法。
- カリウムイオンの濃度が8mMと12mMの間である、請求項1または2に記載の方法。
- 全アミノ酸濃度が40mMと100mMの間である、請求項1から3のいずれか一項に記載の方法。
- 全アミノ酸濃度に対する全イオン濃度のモル比が1.9と4の間である、請求項1から4のいずれか一項に記載の方法。
- 培養条件が少なくとも1つの温度変動および/または少なくとも1つのpH変動を含む、請求項1から5のいずれか一項に記載の方法。
- 培養をフェドバッチプロセスによって行う、請求項1から6のいずれか一項に記載の方法。
- 産生されるポリペプチドがグリコシル化されたものである、請求項1から7のいずれか一項に記載の方法。
- ポリペプチドが抗体または抗体断片である、請求項1から8のいずれか一項に記載の方法。
- 哺乳動物細胞がCHO細胞、HEK細胞およびSP2/0細胞からなる群から選択される、請求項1から9のいずれか一項に記載の方法。
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PCT/EP2011/056508 WO2011134920A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
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MX2012012526A (es) | 2012-11-23 |
KR101828623B1 (ko) | 2018-02-12 |
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RU2012150346A (ru) | 2014-06-10 |
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JP2017012180A (ja) | 2017-01-19 |
IL222452B (en) | 2019-03-31 |
PL2563904T3 (pl) | 2015-06-30 |
US9428727B2 (en) | 2016-08-30 |
BR112012027282A2 (pt) | 2015-09-22 |
DK2563904T3 (en) | 2015-04-07 |
WO2011134920A1 (en) | 2011-11-03 |
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