JP6154606B2 - Antioxidant or whitening cosmetic composition containing an extract of Korean medicine - Google Patents
Antioxidant or whitening cosmetic composition containing an extract of Korean medicine Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/522—Antioxidants; Radical scavengers
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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Description
本発明は、各種の物理的、化学的及び環境的な要因などによって生成される活性酸素による自由ラジカルを消去できる抗酸化用の化粧料組成物、或いは、メラニン色素の沈着を防いで皮膚美白に卓越した効能を有する皮膚美白用の化粧料組成物に係り、さらに詳しくは、韓薬材混合エキスを含有することから、皮膚中の活性酸素による酸化を抑えて抗酸化に卓越した効果があり、しかも、皮膚メラニン色素の沈着を阻害して美白効果に優れた化粧料組成物に関する。 The present invention is an anti-oxidation cosmetic composition capable of eliminating free radicals due to active oxygen generated by various physical, chemical and environmental factors, or skin whitening by preventing the deposition of melanin pigment. It relates to a cosmetic composition for skin whitening that has an excellent effect, and more specifically, it contains a Korean herbal ingredient mixed extract, so it has an excellent antioxidant effect by suppressing oxidation by active oxygen in the skin, Moreover, the present invention relates to a cosmetic composition that inhibits the deposition of skin melanin pigment and has an excellent whitening effect.
体内酵素系、還元代謝、化学薬品、公害物質及び光化学反応などの各種の物理的、化学的及び環境的な要因などによって生成される活性酸素は、細胞構成成分である脂質、タンパク質、糖及びＤＮＡなどに対して非選択的、非可逆的な破壊作用をするため、細胞老化または癌をはじめとする各種の疾病を引き起こすものとして知られている。また、これらの活性酸素による脂質過酸化の結果として生成される脂質過酸化物をはじめとする種々の体内過酸化物も、細胞に対する酸化的な破壊を引き起こして各種の機能障害を来たすため、各種の疾病の原因にもなる。この理由から、このような自由ラジカルを消去できる化合物又は過酸化物の生成抑制物質などの抗酸化剤が、これらの酸化物に起因する老化及び各種の疾患の抑制剤または治療剤として期待される。 Reactive oxygen produced by various physical, chemical and environmental factors such as internal enzyme systems, reductive metabolism, chemicals, pollutants, and photochemical reactions are lipids, proteins, sugars and DNA, which are cell components. It is known to cause various diseases such as cell aging or cancer because of its non-selective and irreversible destructive action. In addition, various body peroxides including lipid peroxides produced as a result of lipid peroxidation by these active oxygens cause various oxidative damages to cells, resulting in various functional disorders. It can also cause illness. For this reason, antioxidants such as compounds capable of eliminating such free radicals or peroxide formation inhibitors are expected as inhibitors or therapeutic agents for aging and various diseases caused by these oxides. .
一方、人間の皮膚色の決定には種々の要因が関与するが、中でも、メラニン色素を生成するメラノサイトの活動性、血管の分布、皮膚の厚さ及びカロテノイド、ビリルビンなどの人体内外の色素含有のいかんなどの要因が重要である。 On the other hand, various factors are involved in the determination of human skin color. Among them, the activity of melanocytes that produce melanin pigment, the distribution of blood vessels, skin thickness and carotenoid, bilirubin and other pigment containing substances inside and outside the human body. Factors such as anger are important.
中でも、最も重要な要因は、人体内のメラノサイトにおいてチロシナーゼなどの種々の酵素が作用して生成されるメラニンという黒色色素である。このメラニン色素の形成には、遺伝的な要因、ホルモン分泌、ストレスなどと関わる生理的要因及び紫外線の照射などの環境的要因などが影響を及ぼす。 Among them, the most important factor is a black pigment called melanin produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. Genetic factors, hormone secretion, physiological factors related to stress, and environmental factors such as ultraviolet irradiation influence the formation of melanin pigment.
身体皮膚のメラニン細胞において生成されるメラニン色素は、黒い色素とタンパク質との複合体の形を有するフェノール系高分子物質であり、太陽から照射される紫外線を遮断して真皮以下の皮膚器官を保護すると共に、皮膚生体内にできた自由ラジカルなどを消去するなど皮膚内タンパク質及び遺伝子を保護する有用な役割を果たす。 The melanin pigment produced in the melanocytes of the body skin is a phenolic polymer substance in the form of a complex of black pigment and protein, which protects the skin organs below the dermis by blocking the ultraviolet rays irradiated from the sun. In addition, it plays a useful role in protecting proteins and genes in the skin, such as by eliminating free radicals formed in the skin body.
このように、皮膚の内外部のストレス的な刺激によってできたメラニンは、ストレスが無くなっても皮膚角質化により外部に排出されないからには消えない安定的な物質である。しかしながら、メラニンが過剰生成されれば、しみやそばかす、斑点などの過色素沈着症を引き起こして美容上の観点でも悪い結果を来たす。 Thus, melanin produced by stressful stimulation inside and outside the skin is a stable substance that does not disappear even if stress is lost, because it is not discharged to the outside due to skin keratinization. However, excessive production of melanin causes hyperpigmentation such as stains, freckles, and spots, and has bad results from a cosmetic point of view.
また、レジャー人口の増大に伴い、アウトドア活動を楽しむ人口が増え、これに伴い、紫外線によるメラニン色素の沈着を防ごうとする要求が高まりつつある。 In addition, with the increase in the leisure population, the number of people who enjoy outdoor activities has increased, and accordingly, there has been an increasing demand to prevent the deposition of melanin pigment by ultraviolet rays.
このような要求に応えて、従来より、アスコルビン酸、コウジ酸、アルブチン、ヒドロキノン、グルタチオン若しくはこれらの誘導体、又はチロシナーゼ阻害活性を有する物質を、化粧料や医薬品に配合して用いてきているが、これらの不十分な美白効果、皮膚使用に対する安全性の問題、化粧料への配合時の剤型及び安定性の問題などによりその使用が制限されている。 In response to such demands, conventionally, ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione or derivatives thereof, or substances having tyrosinase inhibitory activity have been used in cosmetics and pharmaceuticals. Their use is limited by these insufficient whitening effects, safety issues for skin use, dosage forms when formulated into cosmetics, and stability issues.
細辛（サイシン）は、ウマノスズクサ科カンアオイ属の多年草であり、根茎は節が多く、主幹から２枚の葉が向き合ってつく（対生）。生薬の細辛は、薄葉細辛の根を乾燥させたものである。名前の由来は、中国で根が細く、味はきわめて辛いということから細辛といわれる。洗心、▲曾▼、少辛、細草とも呼ばれる。葉は心臓形をしており、幅は５〜１０ｃｍであり、縁部はのっべりしている。２枚の葉の間から１本の花柄を出し、紫褐色の花を咲かせる。実は漿果状であり、先に花被裂片がついており、種子が２０個ほど入っている。根は５〜７月ころに採取して日陰で乾燥させる。薬性は温和で、噛むとピリッとした辛味がある。細辛は、風邪で鼻が詰まっていたり、鼻汁が流れたり、咽喉部から分泌物が排出され続けたりするときに効き目がある。また、汗がよく出ないときに発汗させ、病原菌を体外に排出する。なお、風邪で熱が酷く、特に頭痛が酷くて耐えられないときに役立つ。慢性気管支炎や気管支拡張症によって大量の痰を排出しながら酷い咳をするときには咳止め作用もする。これらに加えて、口内炎にかかったときに細辛の粉末を吹き付けると直ぐ直る。但し、気力の衰弱が激しく、多量の汗をかいたり、貧血で頭痛があるときには使用してはならず、シュロソウと一緒に配合してもならない。民間では、歯が痛いときに噛んでいると、すぐ鎮痛されるとして、鎮痛剤として用いられる。代表的な処方としては、麻黄附子細辛湯がある。 Spicy is a perennial plant belonging to the genus Anthony, which has many nodules and two leaves facing each other from the main trunk. Herb spicy herbs are dried thin roots of thin leaves. The origin of the name is said to be fine, because the roots are thin in China and the taste is extremely spicy. Also called senshin, ▲ 曾 ▼, spicy, fine grass. The leaves have a heart shape, are 5-10 cm wide and have a smooth edge. One flower pattern is put out between the two leaves, and purple-brown flowers bloom. The fruit is actually berry-like, with a flower fragment on top, and about 20 seeds. Roots are collected around May to July and dried in the shade. The medicinal properties are mild and have a tangy pungent taste when chewed. Spicy spicy is effective when the nose is clogged with a cold, nasal discharge flows, and secretions continue to be discharged from the throat. In addition, when sweat does not come out well, it sweats and discharges pathogenic bacteria outside the body. It is useful when you have a cold and a lot of heat, especially when you have a severe headache. When coughing severely while draining a large amount of sputum due to chronic bronchitis or bronchiectasis, it also works as a cough. In addition to these, spraying with a fine powder when you have stomatitis corrects it. However, it should not be used if you are very weak and have a lot of sweat, or are anemic and have a headache. In the private sector, it is used as an analgesic because it is immediately analgesic if it is chewed when the tooth hurts. As a typical prescription, there is Mao-bushi-shoshin-to.
川▲弓▼（センキュウ）は、セリ科ハマゼリ属（クニディム属）の多年草であり、川▲弓▼（センキュウ）、▲弓▼▲窮▼（キュウキュウ）という語で使われる。学名はCnidium officinale MAKINOである。中国産の植物であって、韓国でよく栽培されているが、川▲弓▼は、草丈３０〜６０センチくらいになり、葉は２枚羽状複葉で、小葉は卵状皮針形で、深くさけ、へりにはこまかな鋸歯がある。花は、茎の先に、複合散形花序に小さな多数の白色花をつける。秋の終わりころに果実をつけるが、ほとんどが未熟になり、繁殖は株分けで行う。全草に、特有の芳香があるが、特に根茎の部分には、強い芳香がある。成分は、芳香性精油を多量含有しており、香りがいい。主成分としては、リグスチリド（ligustilide）、クニジリド（cnidilide）、ネオクニジリド（neocnidilide）などがある。植え付けの翌年の秋には掘り取り、ひげ根を取り去って、水洗いして乾燥させる。生薬業者は湯通しという方法で乾燥して仕上げるが、一般にはそのまま乾燥させる。乾燥しにくいので、生のうちに薄く切ってから、日干しにして乾燥させる。これを生薬で、川▲弓▼という。特に、川▲弓▼は虫がつきやすいので、保存には十分な注意が必要である。動物を用いた薬理的な作用実験で、中枢神経系統に作用し、鎮静効果があることを確認した。また、弱いとはいえ、持続的な血圧降下作用も確認された。平滑筋に対する実験では、子宮の収縮力を増大させ、また、殺菌作用があるため大腸菌、赤痢菌、緑膿菌、皮膚真菌の発育を抑制する効果が現れた。この薬は、薬性が温和であり、味が酸っぱいため体内に入って血液の循環をよくし、痛みを鎮める鎮静効果がある。このため、頭痛にも応用される。漢方では、婦人病の要薬とされており、月経不順、月経痛、産後腹痛及び目眩症状をなくす。また、肝臓の機能を活性化させ、貧血時に服用すれば、補血作用がある。そして、心臓筋肉の収縮不全による心臓部位の痛症においても症状を好転させる。腰と脚の骨格が弱く、筋肉に痙攣をよく引き起こす人が服用すれば、そのような症状が改善される。黄連との配合を嫌い、長期間服用すれば、元気を損なう。民間では、衣装だんすに入れてセイヨウシミを予防する。代表的な処方として、▲弓▼歸湯があるが、婦人の出産前後に多用される。川▲弓▼は、漢方で多用される生薬の一つである。 The river ▲ bow ▼ (senkyu) is a perennial plant belonging to the genus Ceramaceae genus (Kunidim genus), and is used in the terms of the river ▲ bow ▼ (senkyu) and ▲ bow ▼ ▲ ▼ (cucumber). The scientific name is Cnidium officinale MAKINO. It is a Chinese plant that is often cultivated in Korea, but the river “bow” has a plant height of 30-60 cm, the leaves are double-leafed and the leaflets are oval skin needles and deeply ablated. The edge has fine saw blades. The flower attaches a number of small white flowers to the complex diffuse inflorescence at the tip of the stem. Fruits are added around the end of autumn, but most of them are immature and breeding is done by stock splitting. The whole plant has a unique fragrance, but there is a strong fragrance especially in the rhizome part. Ingredients contain a large amount of aromatic essential oil and have a good aroma. Examples of the main component include ligustilide, cnidilide, neocnidilide and the like. In the fall of the year after planting, it is dug, the roots are removed, washed with water and dried. Herbal medicines are finished by drying with a method called blanching, but generally they are dried as they are. Since it is difficult to dry, cut it thinly in the raw, and then dry it in the sun. This is herbal medicine and is called the river “bow”. In particular, the river “bow” is easy to get insects, so you need to be careful when you save it. In a pharmacological action experiment using animals, it was confirmed that it acts on the central nervous system and has a sedative effect. Moreover, although it was weak, the continuous blood pressure lowering effect was confirmed. In experiments on smooth muscle, the effect of suppressing the growth of Escherichia coli, Shigella, Pseudomonas aeruginosa, and skin fungi appeared due to the increased uterine contractility and bactericidal action. This medicine has mild pharmacological properties and has a soothing taste, so it enters the body, improves blood circulation, and has a sedative effect to relieve pain. For this reason, it is also applied to headaches. In Kampo, it is regarded as an essential drug for gynecological diseases, and it eliminates irregular menstruation, menstrual pain, postpartum abdominal pain and dizziness. In addition, if the liver function is activated and taken during anemia, it has a blood-supplementing effect. Symptoms are also improved in heart region pain due to cardiac muscle contraction failure. If taken by a person with weak back and leg skeletons and often causing muscle spasms, such symptoms are improved. Dislikes the combination with Huang Ren, and if you take it for a long time, it will harm your spirit. In the private sector, put it in a costume wardrobe to prevent lice. A typical prescription is ▲ bow ▼ 歸 yu, which is frequently used before and after a woman's childbirth. River “bow” is one of herbal medicines frequently used in Chinese medicine.
杏仁は、中国産のバラ科に属するマンシュウアンズ、モウコアンズの乾燥種子である。鎮咳及び去痰効能があるため、民間では、咳嗽、喘息、気管支炎、急性肝炎などに用いられる。杏仁の主な成分としては、クエン酸、リンゴ酸、アミグダリン、オレインなどがあり、これらに加えて、特に、カリウムとリンを多量含有しており、糖質、カルシウム、ナトリウム、繊維質、ビタミンＡ、Ｂ、Ｃなどを含有している。荒れて乾燥した皮膚に潤いと栄養を与え、小皺、しみ、そばかすの除去、美白、角質除去の効果がある。本草綱目には、杏仁を用いた治療方法が２００余種類も記録されているほどその用途が多く、薬効も高い。杏仁をすりおろして作ったパックは、主として、しみ、そばかすなど皮膚色素の沈着、はれ物などに用いられる。 Anjin is a dried seed of Manchuanzu, Maucoans belonging to the family Rosaceae from China. Because of its antitussive and expectorant effect, it is used in the private sector for cough, asthma, bronchitis, acute hepatitis, and the like. The main ingredients of apricot are citric acid, malic acid, amygdalin, olein, etc. In addition to these, it contains a large amount of potassium and phosphorus, especially carbohydrates, calcium, sodium, fiber, vitamin A. , B, C and the like. Moisturizes and nourishes rough and dry skin and has the effects of removing wrinkles, stains, freckles, whitening and exfoliating. In this class, more than 200 kinds of treatment methods using apricot kernel are recorded, and the use is more and the medicinal effect is higher. Packs made by grated anzujin are mainly used for the deposition of skin pigments such as stains and freckles, and for exfoliation.
そこで、本発明者らは、前記問題点を解消し、より優れた抗酸化及び美白原料を見出そうとする研究の一環として、種々の天然物を検索したところ、細辛、川▲弓▼及び杏仁からなる韓薬材混合エキスが単体のエキスに比べて抗酸化及び美白効果に非常に優れているということを見出し、本発明を完成するに至った。 Therefore, the present inventors searched various natural products as part of research to solve the above problems and find better antioxidant and whitening materials. As a result, the present inventors have found that a Korean herbal medicine mixture extract consisting of Apricot and Jinjin is extremely superior in antioxidant and whitening effects as compared with a single extract.
そこで、本発明の目的は、細辛エキス、川▲弓▼エキス及び杏仁エキスを有効成分として含有する化粧料組成物を提供することである。本発明の他の目的は、細辛エキス、川▲弓▼エキス及び杏仁エキスを有効成分として含有して抗酸化作用を有する化粧料組成物を提供することである。本発明のさらに他の目的は、細辛エキス、川▲弓▼エキス及び杏仁エキスを有効成分として含有して自由ラジカル消去能を有する化粧料組成物を提供することである。本発明のさらに他の目的は、細辛エキス、川▲弓▼エキス及び杏仁エキスを有効成分として含有して皮膚美白効能を有する化粧料組成物を提供することである。 Accordingly, an object of the present invention is to provide a cosmetic composition containing as an active ingredient a spicy extract, a river bow extract and an apricot extract. Another object of the present invention is to provide a cosmetic composition having an antioxidative effect, comprising a spicy extract, a river bow extract and an apricot extract as active ingredients. Still another object of the present invention is to provide a cosmetic composition having free radical scavenging ability, containing a spicy extract, a river bow extract and an apricot extract as active ingredients. Still another object of the present invention is to provide a cosmetic composition having a skin whitening effect, containing a spicy extract, a river bow extract and an apricot extract as active ingredients.
上述した目的を達成するために、本発明は、細辛エキス、川▲弓▼エキス及び杏仁エキスを有効成分として含有する化粧料組成物を提供する。 In order to achieve the above-described object, the present invention provides a cosmetic composition containing a spicy extract, a river bow extract and an apricot extract as active ingredients.
以下、本発明に係る韓薬材混合エキスを含有する抗酸化用または美白用の化粧料組成物について詳述する。 Hereinafter, the anti-oxidant or whitening cosmetic composition containing the Hankook material mixture extract according to the present invention will be described in detail.
本発明の細辛エキス、川▲弓▼エキス及び杏仁エキスは、当業界における通常の方法により得られるものであり、その方法に特に限定はない。具体的には、乾燥された細辛、川▲弓▼又は杏仁にエタノールを加えて油溶性成分を抽出し、還流抽出後に沈漬し、ろ過及び遠心分離により分離されたろ液を減圧・濃縮して、細辛エキス、川▲弓▼エキス又は杏仁エキスとして得る。 The spicy extract, river bow extract and apricot extract of the present invention are obtained by a conventional method in the art, and the method is not particularly limited. Specifically, ethanol is added to dried dried spicy fish, river archery or apricot kernel to extract oil-soluble components, submerged after reflux extraction, and the filtrate separated by filtration and centrifugation is decompressed and concentrated. And obtained as a spicy extract, river ▲ bow ▼ extract or apricot kernel extract.
本発明の細辛、川▲弓▼及び杏仁の混合エキスは、組成物の総質量に対して０．００１〜２０質量％、より好ましくは、０．００５〜１０質量％含有される。０．００１質量％未満では、所望の効果を期待することができず、２０質量％を超える場合には、変臭など化粧品の変性が起こる虞があり、化粧品剤型の粘度が調節し難く、エマルジョン剤型の製造時に転相が起こり易いため化粧料の剤型として開発し難い他、経済性にも劣る。 The mixed extract of the spicy fish, the river bow and the anion of the present invention is contained in an amount of 0.001 to 20% by mass, more preferably 0.005 to 10% by mass, based on the total mass of the composition. If it is less than 0.001% by mass, the desired effect cannot be expected, and if it exceeds 20% by mass, there is a possibility that cosmetic modification such as deodorization may occur, and the viscosity of the cosmetic preparation is difficult to adjust. Since phase inversion tends to occur during the production of an emulsion dosage form, it is difficult to develop it as a cosmetic dosage form and it is inferior in economic efficiency.
本発明の化粧料組成物に含有されている細辛エキス、川▲弓▼エキス及び杏仁エキスの混合比は、質量比にて１：１〜３：１〜３である。混合比が上記の範囲から外れると、成分の相乗効果が期待し難い。 The mixing ratio of the spicy extract, the river “bow” extract and the apricot extract contained in the cosmetic composition of the present invention is 1: 1 to 3: 1 to 3 in mass ratio. If the mixing ratio is out of the above range, it is difficult to expect a synergistic effect of the components.
本発明において提供する化粧料組成物は、上記の細辛、川▲弓▼及び杏仁の混合エキスを含有することにより、紫外線、その他の外部有害成分から誘発される皮膚酸化反応を遮断する効果があり、しかも、皮膚メラニン色素の沈着を阻害して美白効果を実質的に実現することができる。 The cosmetic composition provided in the present invention has the effect of blocking the skin oxidation reaction induced by ultraviolet rays and other external harmful components by containing the mixed extract of the above-mentioned spicy, river bow and apricot kernel. In addition, the skin whitening effect can be substantially realized by inhibiting the deposition of skin melanin pigment.
本発明の化粧料組成物は、皮膚抗酸化用及び美白用の化粧品に適用可能であり、その剤型には特に制限がなく、例えば、乳液、クリーム、化粧水、エッセンス、パック、ジェル、パウダー、口紅、メイクアップベース、ファウンデーション、ローション又は皮膚粘着型の化粧料の剤型あるいは経皮投与型の剤型であってもよい。 The cosmetic composition of the present invention can be applied to cosmetics for skin antioxidants and whitening, and there is no particular limitation on the dosage form, for example, emulsion, cream, lotion, essence, pack, gel, powder. Lipsticks, makeup bases, foundations, lotions or skin-adhesive cosmetic dosage forms or transdermal dosage forms.
本発明においては、細辛エキス、川▲弓▼エキス及び杏仁エキスを含有する化粧料組成物が、ＤＰＰＨの生成を抑える抗酸化効果があり、しかも、紫外線（ＵＶ）によって生成された色素沈着を改善する効果に起因する美白効果があることを確認した。このため、本発明に係る細辛、川▲弓▼及び杏仁の混合エキスは、抗酸化用または美白用の化粧料組成物若しくは薬学組成物として非常に有用である。 In the present invention, the cosmetic composition containing the spicy extract, the river bow extract, and the apricot extract has an antioxidant effect that suppresses the production of DPPH, and also exhibits pigmentation produced by ultraviolet (UV). It was confirmed that there was a whitening effect due to the improving effect. For this reason, the mixed extract of spicy, river, and apricot kernels according to the present invention is very useful as a cosmetic or pharmaceutical composition for antioxidant or whitening.
以下、実施例及び試験例を挙げて本発明について詳述するが、本発明の権利範囲がこれらによって限定されることはない。 EXAMPLES Hereinafter, although an Example and a test example are given and this invention is explained in full detail, the right scope of this invention is not limited by these.
［比較例１］細辛エキスの製造
乾燥させた細辛３００ｇに７０％エタノール２ｌを入れ、３回に亘って還流抽出を行った後、１５℃において１日間沈漬した。次いで、ろ過布によるろ過及び遠心分離により残渣とろ液を分離し、分離されたろ液を減圧濃縮してエキス２４ｇを得た。
[Comparative Example 1] Manufacture of a fine spicy extract 2 l of 70% ethanol was added to 300 g of dried spicy extract, subjected to reflux extraction three times, and then submerged at 15 ° C for 1 day. Subsequently, the residue and the filtrate were separated by filtration with a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 24 g of an extract.
［比較例２］川▲弓▼エキスの製造
乾燥させた川▲弓▼３００ｇに７０％エタノール２ｌを入れ、３回に亘って還流抽出を行った後、１５℃において１日間沈漬した。次いで、ろ過布によるろ過及び遠心分離により残渣とろ液を分離し、分離されたろ液を減圧濃縮してエキス３３ｇを得た。
[Comparative Example 2] Manufacture of River Bow Extract 70 g of 70% ethanol was added to 300 g of dried river bow and subjected to reflux extraction three times, followed by submerging at 15 ° C for 1 day. Subsequently, the residue and the filtrate were separated by filtration with a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 33 g of an extract.
［比較例３］杏仁エキスの製造
乾燥させた杏仁３００ｇに７０％エタノール２ｌを入れ、３回に亘って還流抽出を行った後、１５℃において１日間沈漬した。次いで、ろ過布によるろ過及び遠心分離により残渣とろ液を分離し、分離されたろ液を減圧濃縮してエキス３５ｇを得た。
[Comparative Example 3] Manufacture of apricot jelly extract 2 l of 70% ethanol was added to 300 g of dried apricot jelly and subjected to reflux extraction three times, followed by submerging at 15 ° C for 1 day. Subsequently, the residue and the filtrate were separated by filtration with a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 35 g of an extract.
［実施例１］細辛、川▲弓▼及び杏仁の混合エキスの製造
比較例１の細辛エキス２０ｇ、比較例２の川▲弓▼エキス２０ｇ及び比較例３の杏仁エキス２０ｇを混合し、７０％エタノール水溶液４００ｍｌを入れて完全に溶解させた後、１５℃において１日間沈漬した。次いで、ろ過布によるろ過及び遠心分離により残渣とろ液を分離し、分離されたろ液を減圧濃縮して細辛、川▲弓▼及び杏仁の混合エキス４６ｇを得た。
[Example 1] Manufacture of mixed extract of spicy, river ▲ bow and apricot kernel 20 g of spicy extract of comparative example 1, 20 g of river ▲ bow extract of comparative example 2 and 20 g of apricot extract of comparative example 3, After adding 400 ml of 70% ethanol aqueous solution and completely dissolving, it was submerged at 15 ° C. for 1 day. Next, the residue and the filtrate were separated by filtration with a filter cloth and centrifugal separation, and the separated filtrate was concentrated under reduced pressure to obtain 46 g of a mixed extract of spicy, river and bow.
［実施例２］細辛、川▲弓▼及び杏仁の混合エキスの製造
比較例１の細辛エキス１２ｇ、比較例２の川▲弓▼エキス３６ｇ及び比較例３の杏仁エキス１２ｇを混合し、７０％エタノール水溶液４５０ｍｌを入れて完全に溶解させた後、１５℃において１日間沈漬した。次いで、ろ過布によるろ過及び遠心分離により残渣とろ液を分離し、分離されたろ液を減圧濃縮して細辛、川▲弓▼及び杏仁の混合エキス４９ｇを得た。
[Example 2] Manufacture of mixed extract of spicy, river ▲ bow and apricot kernel 12 g of spicy extract of comparative example 1, 36 g of river ▲ bow extract of comparative example 2 and 12 g of apricot extract of comparative example 3, After 450 ml of 70% ethanol aqueous solution was added and completely dissolved, it was immersed at 15 ° C. for 1 day. Subsequently, the residue and the filtrate were separated by filtration with a filter cloth and centrifugal separation, and the separated filtrate was concentrated under reduced pressure to obtain 49 g of a mixed extract of fine spicy, river arch and anpricot kernel.
［実施例３］細辛、川▲弓▼及び杏仁の混合エキスの製造
比較例１の細辛エキス１２ｇ、比較例２の川▲弓▼エキス１２ｇ及び比較例３の杏仁エキス３６ｇを混合し、７０％エタノール水溶液４５０ｍｌを入れて完全に溶解させた後、１５℃において１日間沈漬した。次いで、ろ過布によるろ過及び遠心分離により残渣とろ液を分離し、分離されたろ液を減圧濃縮して細辛、川▲弓▼及び杏仁の混合エキス４８ｇを得た。
[Example 3] Manufacture of mixed extract of spicy, river ▲ bow and apricot kernel 12 g of spicy extract of Comparative Example 1, 12 g of a river 弓 bow extract of Comparative Example 2 and 36 g of apricot kernel extract of Comparative Example 3 were mixed, After 450 ml of 70% ethanol aqueous solution was added and completely dissolved, it was immersed at 15 ° C. for 1 day. Subsequently, the residue and the filtrate were separated by filtration with a filter cloth and centrifugal separation, and the separated filtrate was concentrated under reduced pressure to obtain 48 g of a mixed extract of fine spicy fish, river bow and anpricot kernel.
［実施例４］細辛、川▲弓▼及び杏仁の混合エキスの製造
比較例１の細辛エキス８．５ｇ、比較例２の川▲弓▼エキス２５．５ｇ及び比較例３の杏仁エキス２５．５ｇを混合し、７０％エタノール水溶液４５０ｍｌを入れて完全に溶解させた後、１５℃において１日間沈漬した。次いで、ろ過布によるろ過及び遠心分離により残渣とろ液を分離し、分離されたろ液を減圧濃縮して細辛、川▲弓▼及び杏仁の混合エキス４８ｇを得た。
[Example 4] Manufacture of mixed extract of spicy, river ▲ bow and apricot seed 8.5 g of spicy extract of comparative example 1, 25.5 g of a river arch of comparative example 2 and apricot extract 25 of comparative example 3 0.5 g was mixed, 450 ml of 70% ethanol aqueous solution was added and completely dissolved, and then immersed at 15 ° C. for 1 day. Subsequently, the residue and the filtrate were separated by filtration with a filter cloth and centrifugal separation, and the separated filtrate was concentrated under reduced pressure to obtain 48 g of a mixed extract of fine spicy fish, river bow and anpricot kernel.
［試験例１］抗酸化効果試験（ＤＰＰＨテスト）
有機ラジカルであるＤＰＰＨ（1,1-diphenyl-2-picryl hydrazyl）の還元により（抗酸化剤は酸化される）発生される吸光度の変化により抗酸化能を評価する方法を用いた。下記の例によってＤＰＰＨの酸化が抑制されて吸光度が対照群に比べて減少される度合いを測定して、対照群の吸光度に比べて５０％以下の吸光度を示す濃度を有効抗酸化の濃度として評価した。
[Test Example 1] Antioxidant effect test (DPPH test)
A method was used in which the antioxidant ability was evaluated by the change in absorbance generated by the reduction of the organic radical DPPH (1,1-diphenyl-2-picryl hydrazyl) (the antioxidant is oxidized). The following example measures the degree to which the DPPH oxidation is suppressed and the absorbance is decreased compared to the control group, and evaluates the concentration that exhibits an absorbance of 50% or less as compared to the control group as the effective antioxidant concentration. did.
１００μＭ（エタノール中）ＤＰＰＨ溶液１９０μｌと、前記比較例、実施例のエキス１〜４と、比較試料及び対照試料をそれぞれ１０μｌ入れて反応液を製造し、３７℃において３０分間反応させた後、５４０ｎｍにおける吸光度を測定した。対照試料としては、汎用されている合成抗酸化剤トロロクス（Trolox）を用いた。 A reaction solution was prepared by adding 190 μl of a 100 μM (in ethanol) DPPH solution, 10 μl of each of Comparative Examples and Examples 1 to 4 above, a comparative sample and a control sample, reacted at 37 ° C. for 30 minutes, and then reacted at 540 nm. The absorbance at was measured. As a control sample, a commonly used synthetic antioxidant, Trolox, was used.
各物質のＤＰＰＨ分析結果を下記表１に示す。 The results of DPPH analysis for each substance are shown in Table 1 below.
表１から明らかなように、実施例１〜４に従い製造した本発明に係る細辛、川▲弓▼及び杏仁の混合エキスの方が、比較例の単体のエキスよりも良好な活性を示し、当業界における公知の抗酸化剤であるトロロクスとほとんど同じ抗酸化能を示す。 As can be seen from Table 1, the mixed extract of spicy, river 弓 and annin produced according to the present invention according to Examples 1 to 4 showed better activity than the single extract of the comparative example, It exhibits almost the same antioxidant ability as Trolox, a known antioxidant in the industry.
［試験例２］ラットの色素細胞を用いたメラニン生成抑制効果の測定
前記比較例及び実施例に従い製造した混合エキスと対照試料のメラニン生成抑制効果を調べるために、ラットの色素細胞を用いて測定した。
[Test Example 2] Measurement of melanin production inhibitory effect using rat pigment cells In order to examine the melanin production inhibitory effect of the mixed extract prepared according to the comparative example and the example and the control sample, measurement was performed using rat pigment cells. did.
先ず、Ｃ５７ＢＬ／６マウス由来のラットの色素細胞（Mel-Ab cell）（Dooley,T.P. et al,Skin pharmacol,7,pp 188-200）を、１０％ウシ胎盤血清、１００ｎＭ１２−Ｏ−テトラデカノイルホルボール−１３−酢酸（12-O-tetradecanoylphorbol-13-acetate）、１ｎＭコレラ毒素入りＤＭＥＭ培地において、３７℃、５％ＣＯ2の条件下で培養した。培養されたＭｅｌ−Ａｂ細胞を０．２５％トリプシン−ＥＤＴＡにより取り、２４−ウェルプレートに１０5細胞／ウェルの濃度にて細胞を培養し、２日目から３日間連続して１０ｐｐｍの各試験物質としてヒドロキノン、前記比較例、実施例に従い製造した混合エキスと対照試料を加えて培養した。このとき、前記ヒドロキノンは、陽性対照群として用いた。次いで、培養液を除去し、ＰＢＳにより洗浄した後、１Ｎ水酸化ナトリウムにより細胞を溶かして４００ｎｍにおける吸光度を測定した後、下記の数式１に従いメラニン生成抑制率を計算し、その結果を表２に示す（ドゥーリーの方法）。 First, rat melanoma cells derived from C57BL / 6 mice (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) were obtained from 10% bovine placental serum, 100 nM12-O-tetradecanoyl. Phorbol-13-acetate (12-O-tetradecanoylphorbol-13-acetate) was cultured in a DMEM medium containing 1 nM cholera toxin under conditions of 37 ° C. and 5% CO 2 . Cultured Mel-Ab cells are taken up with 0.25% trypsin-EDTA, cultured in 24-well plates at a concentration of 10 5 cells / well, and each test at 10 ppm for 3 consecutive days from the second day. Hydroquinone as a substance, mixed extracts prepared according to the comparative examples and examples, and a control sample were added and cultured. At this time, the hydroquinone was used as a positive control group. Next, after removing the culture medium and washing with PBS, the cells were dissolved with 1N sodium hydroxide and the absorbance at 400 nm was measured. Then, the melanin production inhibition rate was calculated according to the following formula 1, and the results are shown in Table 2. Show (Dooley method).
［数式１］
メラニン生成抑制率（％）＝１００−（試験物質の吸光度／対照群の吸光度×１００）
[Formula 1]
Melanin production inhibition rate (%) = 100− (absorbance of test substance / absorbance of control group × 100)
前記表２に示すように、本発明の実施例１〜４により得られた細辛、川▲弓▼及び杏仁の混合エキスの方が、比較例の単体のエキスよりも良好なメラニン生成抑制率を示すことが確認され、特に、混合エキスがヒドロキノンとほとんど同じメラニン生成抑制率を示すことが確認された。 As shown in Table 2, the mixed extract of spicy, river, and apricot kernels obtained in Examples 1 to 4 of the present invention has a better melanin production inhibition rate than the single extract of the comparative example. In particular, it was confirmed that the mixed extract exhibits almost the same melanin production inhibition rate as hydroquinone.
Claims (6)
当該細辛エキス、川▲弓▼エキス及び杏仁エキスの混合比は、質量比にて１：１〜３：１〜３であることを特徴とする化粧料組成物。 As an active ingredient, 0.001 to 20% by mass of a mixed extract composed of a spicy extract, a river ▲ bow ▼ extract, and an apricot extract based on the total mass of the composition ,
A cosmetic composition characterized in that the mixture ratio of the spicy extract, the river 弓 bow extract and the apricot extract is 1: 1 to 3: 1 to 3 in terms of mass ratio .
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| CN105708771A (en) * | 2016-04-25 | 2016-06-29 | 温州博远工业设计有限公司 | Formula and preparation technology of skin-whitening and beautifying effective composition |
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