JP5186676B2 - 細胞の凍結保存のための装置及び方法 - Google Patents
細胞の凍結保存のための装置及び方法 Download PDFInfo
- Publication number
- JP5186676B2 JP5186676B2 JP2009516675A JP2009516675A JP5186676B2 JP 5186676 B2 JP5186676 B2 JP 5186676B2 JP 2009516675 A JP2009516675 A JP 2009516675A JP 2009516675 A JP2009516675 A JP 2009516675A JP 5186676 B2 JP5186676 B2 JP 5186676B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleation
- cryopreservation
- automatic
- container
- ice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000005138 cryopreservation Methods 0.000 title claims description 63
- 238000000034 method Methods 0.000 title description 39
- 238000010899 nucleation Methods 0.000 claims description 47
- 239000007788 liquid Substances 0.000 claims description 39
- 230000006911 nucleation Effects 0.000 claims description 31
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 26
- 238000007710 freezing Methods 0.000 claims description 26
- 230000008014 freezing Effects 0.000 claims description 26
- 235000012000 cholesterol Nutrition 0.000 claims description 13
- 229930182558 Sterol Natural products 0.000 claims description 11
- 150000003432 sterols Chemical group 0.000 claims description 11
- 235000003702 sterols Nutrition 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 9
- 230000006910 ice nucleation Effects 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 239000011159 matrix material Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 2
- 238000000576 coating method Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 51
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 38
- 210000004027 cell Anatomy 0.000 description 36
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 33
- 239000000243 solution Substances 0.000 description 27
- 238000001816 cooling Methods 0.000 description 19
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 14
- 230000008569 process Effects 0.000 description 14
- 230000015572 biosynthetic process Effects 0.000 description 13
- 239000013078 crystal Substances 0.000 description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
- 210000001519 tissue Anatomy 0.000 description 10
- 238000003860 storage Methods 0.000 description 9
- 239000002577 cryoprotective agent Substances 0.000 description 8
- 230000004899 motility Effects 0.000 description 8
- 238000004781 supercooling Methods 0.000 description 8
- 239000000306 component Substances 0.000 description 7
- 235000013601 eggs Nutrition 0.000 description 7
- 210000000130 stem cell Anatomy 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 238000007789 sealing Methods 0.000 description 6
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 5
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 5
- 230000003834 intracellular effect Effects 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 230000007704 transition Effects 0.000 description 5
- 239000008358 core component Substances 0.000 description 4
- 230000000959 cryoprotective effect Effects 0.000 description 4
- 210000004700 fetal blood Anatomy 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 238000010257 thawing Methods 0.000 description 4
- 230000035899 viability Effects 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 244000309464 bull Species 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 208000037887 cell injury Diseases 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000269350 Anura Species 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 1
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 102000012750 Membrane Glycoproteins Human genes 0.000 description 1
- 108010090054 Membrane Glycoproteins Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 210000000991 chicken egg Anatomy 0.000 description 1
- HGAZMNJKRQFZKS-UHFFFAOYSA-N chloroethene;ethenyl acetate Chemical compound ClC=C.CC(=O)OC=C HGAZMNJKRQFZKS-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000004836 empirical method Methods 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000004299 exfoliation Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000012595 freezing medium Substances 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 230000000642 iatrogenic effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 210000005061 intracellular organelle Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 231100000065 noncytotoxic Toxicity 0.000 description 1
- 208000008634 oligospermia Diseases 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000010421 standard material Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Hematology (AREA)
- Mechanical Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medical Preparation Storing Or Oral Administration Devices (AREA)
Description
(関連出願)
本願は、2006年6月20日に出願された“細胞の凍結保存のための装置及び方法”という名称の同時係属の米国仮特許出願第60/814,982号に基づく優先権を主張している。当該出願の全体が本明細書に参考として組み入れられている。
Claims (11)
- 内部に含まれる液体を、凍結に先立って凍結保存容器内へ導入するための自動核形成装置であり、
凍結保存容器内へ導入できる大きさとされた細長い中空の管と、
当該中空の管内に配置された氷核形成成分と、
前記中空の管の両端に設けられたシールと、を備え、
前記両端のうちの少なくとも一方の端部は前記氷核形成成分に対して不透過性であるが前記凍結保存容器内に含まれている液体に対しては透過性である膜によってシールされている、自動核形成装置。 - 請求項1に記載の自動核形成装置であり、
前記氷核形成成分がステロールであることを特徴とする自動核形成装置。 - 請求項2に記載の自動核形成装置であり、
前記氷核形成成分がコレステロールであることを特徴とする自動核形成装置。 - 請求項1に記載の自動核形成装置であり、
前記氷核形成成分が前記中空の管の内側に設けられたコーティングであることを特徴とする自動核形成装置。 - 請求項1に記載の自動核形成装置であり、
前記氷核形成成分が前記管内に設けられた固体マトリックスであることを特徴とする自動核形成装置。 - 請求項1に記載の自動核形成装置であり、
前記中空の管の両端に設けられた前記シールが前記膜であることを特徴とする自動核形成装置。 - 請求項1に記載の自動核形成装置が内部に配設された凍結保存容器。
- 請求項7に記載の凍結保存容器であり、
前記自動核形成装置が前記容器の内部に取り付けられていることを特徴とする凍結保存容器。 - 請求項8に記載の凍結保存容器であり、
可撓性の管状の本体であって、一端が当該本体内へ液体サンプルを導入するために最初は開口している前記可撓性の管状本体と、
当該管状本体の他端に形成されている閉塞されたポートであって、前記液体サンプルの抜き取りのための針によって穿刺されるようになされた前記ポートと、を更に備えていることを特徴とする凍結保存容器。 - 請求項9に記載の凍結保存容器であり、
前記自動の核形成装置が、前記入口からずらされている前記管状本体の内部に固定されていることを特徴とする凍結保存容器。 - 請求項9に記載の凍結保存容器であり、
前記管状本体の前記一端が熱シール可能な材料によって作られていることを特徴とする凍結保存容器。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US81498206P | 2006-06-20 | 2006-06-20 | |
US60/814,982 | 2006-06-20 | ||
PCT/US2007/071545 WO2007149847A2 (en) | 2006-06-20 | 2007-06-19 | Systems and methods for cryopreservation of cells |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012053369A Division JP2012152217A (ja) | 2006-06-20 | 2012-03-09 | 細胞の凍結保存のための装置及び方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2009540833A JP2009540833A (ja) | 2009-11-26 |
JP5186676B2 true JP5186676B2 (ja) | 2013-04-17 |
Family
ID=38834305
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009516675A Active JP5186676B2 (ja) | 2006-06-20 | 2007-06-19 | 細胞の凍結保存のための装置及び方法 |
JP2012053369A Pending JP2012152217A (ja) | 2006-06-20 | 2012-03-09 | 細胞の凍結保存のための装置及び方法 |
JP2014075300A Active JP5925826B2 (ja) | 2006-06-20 | 2014-04-01 | 細胞の凍結保存のための装置及び方法 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012053369A Pending JP2012152217A (ja) | 2006-06-20 | 2012-03-09 | 細胞の凍結保存のための装置及び方法 |
JP2014075300A Active JP5925826B2 (ja) | 2006-06-20 | 2014-04-01 | 細胞の凍結保存のための装置及び方法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US7939316B2 (ja) |
EP (2) | EP3351100B1 (ja) |
JP (3) | JP5186676B2 (ja) |
BR (1) | BRPI0713683B1 (ja) |
CA (1) | CA2656139C (ja) |
ES (1) | ES2969987T3 (ja) |
WO (1) | WO2007149847A2 (ja) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8709797B2 (en) * | 2006-06-20 | 2014-04-29 | Cook General Biotechnology Llc | Systems and methods for cryopreservation of cells |
US8222027B2 (en) | 2006-06-20 | 2012-07-17 | Cook General Biotechnolgy, LLC | Systems and methods for cryopreservation of cells |
WO2010111255A1 (en) * | 2009-03-23 | 2010-09-30 | Hememics Biotechnologies, Inc. | Desiccated biologics and methods of preparing the same |
WO2010127158A1 (en) * | 2009-04-29 | 2010-11-04 | Biolife Solutions, Inc. | Apparatuses and compositions for cryopreservation of cellular monolayers |
AT508582B1 (de) * | 2009-07-01 | 2011-09-15 | Leica Mikrosysteme Gmbh | Verfahren zur herstellung einer in einen probenbehälter eingeschlossenen wasserhaltigen probe und probenbehälter zur durchführung des verfahrens |
CN102160546B (zh) * | 2010-02-24 | 2016-01-20 | 库克通用生物科技有限责任公司 | 冷冻保存细胞的***和方法 |
DE102011108251A1 (de) | 2011-07-22 | 2013-01-24 | Gottfried Wilhelm Leibniz Universität Hannover, Körperschaft des öffentlichen Rechts | Verfahren zum Induzieren der Nukleation in einer Probe und System hierfür |
GB201506541D0 (en) * | 2015-04-16 | 2015-06-03 | Lamb S | Improved methods for cryopreservation of biological materials |
WO2019084371A1 (en) | 2017-10-26 | 2019-05-02 | Cook Regentec Llc | ADAPTER FOR BOTTLES AND SYRINGES |
WO2019152823A1 (en) | 2018-02-01 | 2019-08-08 | Cook Regentec Llc | Closed system syringe loading |
CN108277152B (zh) * | 2018-04-04 | 2023-10-13 | 博品(上海)生物医药科技有限公司 | 细胞复苏用无菌换液装置及利用该装置进行细胞复苏换液的方法 |
US11116206B2 (en) | 2018-10-01 | 2021-09-14 | Cook Medical Technologies Llc | Cryocontainer |
WO2020198534A1 (en) | 2019-03-26 | 2020-10-01 | Sicreations, Llc | Imaging agents and methods of using the same |
EP3976117A4 (en) * | 2019-05-29 | 2023-02-01 | Sicreations, LLC | METHODS OF CONSERVING A SUBJECT AND USING IMAGING CONTRAST AGENTS |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2653606A (en) * | 1949-07-06 | 1953-09-29 | George R Ryan | Transfusion apparatus |
US4262494A (en) * | 1980-01-30 | 1981-04-21 | Karow Jr Armand M | Portable device for cryopreservation, transportation and temporary cryogenic storage of semen and other similar tissue |
GB8611894D0 (en) * | 1986-05-15 | 1986-06-25 | Cell Systems Ltd | Biological cryo-protection |
US4928493A (en) | 1987-02-06 | 1990-05-29 | Reaction Thermal Systems, Inc. | Ice building, chilled water system and method |
CA2064728A1 (en) * | 1989-08-11 | 1991-02-12 | Brian William Wilson Grout | Biological cryopreservation |
GB8918370D0 (en) * | 1989-08-11 | 1989-09-20 | Cell Systems Ltd | Material for use in biological cryoprotection |
AU653708B2 (en) * | 1989-08-11 | 1994-10-13 | Cell Systems Limited | Biological cryopreservation |
FR2651793B1 (fr) * | 1989-09-14 | 1991-12-06 | Cassou Robert | Tubes ou paillettes pour la conservation cryogenique d'echantillons biologiques tels que virus, et procede de remplissage. |
US5460625A (en) * | 1990-07-31 | 1995-10-24 | Baxter International Inc. | Cryogenic resistant coextruded tubing |
US6337205B1 (en) * | 1998-01-06 | 2002-01-08 | Integrated Biosystems, Inc | Cryopreservation vial apparatus and methods |
JP3776227B2 (ja) * | 1998-01-16 | 2006-05-17 | テルモ株式会社 | 採血用器具 |
US6592613B1 (en) * | 1998-01-16 | 2003-07-15 | Terumo Kabushiki Kaisha | Blood collecting apparatus and blood collecting method using blood collecting apparatus |
JP4030682B2 (ja) * | 1999-07-09 | 2008-01-09 | テルモ株式会社 | 採血用器具 |
CN1585894B (zh) * | 2000-12-07 | 2010-08-04 | 弗劳恩霍弗应用技术研究院 | 冷储存的方法和装置 |
DE10134913C2 (de) * | 2001-07-18 | 2003-06-26 | Hiberna Ag | Vorrichtung und Verfahren zur Blutpräparation |
US7727219B2 (en) * | 2001-10-22 | 2010-06-01 | Vita 34 Ag | Sterile system and methods for collecting, transporting, storing and cyropreserving body fluids |
JP2004236527A (ja) * | 2003-02-04 | 2004-08-26 | Asahi Kasei Corp | 有核細胞分離装置 |
WO2005035737A1 (ja) * | 2003-10-10 | 2005-04-21 | Asahi Kasei Medical Co., Ltd. | 細胞濃縮物の調製方法および細胞組成物 |
US8222027B2 (en) * | 2006-06-20 | 2012-07-17 | Cook General Biotechnolgy, LLC | Systems and methods for cryopreservation of cells |
US8709797B2 (en) * | 2006-06-20 | 2014-04-29 | Cook General Biotechnology Llc | Systems and methods for cryopreservation of cells |
-
2007
- 2007-06-19 EP EP18151806.9A patent/EP3351100B1/en active Active
- 2007-06-19 BR BRPI0713683A patent/BRPI0713683B1/pt not_active IP Right Cessation
- 2007-06-19 US US11/765,000 patent/US7939316B2/en active Active
- 2007-06-19 CA CA2656139A patent/CA2656139C/en active Active
- 2007-06-19 WO PCT/US2007/071545 patent/WO2007149847A2/en active Application Filing
- 2007-06-19 EP EP07812200.9A patent/EP2038402B1/en active Active
- 2007-06-19 JP JP2009516675A patent/JP5186676B2/ja active Active
- 2007-06-19 ES ES18151806T patent/ES2969987T3/es active Active
-
2012
- 2012-03-09 JP JP2012053369A patent/JP2012152217A/ja active Pending
-
2014
- 2014-04-01 JP JP2014075300A patent/JP5925826B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
US20080003561A1 (en) | 2008-01-03 |
EP2038402A4 (en) | 2012-12-05 |
BRPI0713683B1 (pt) | 2015-12-01 |
EP3351100B1 (en) | 2023-10-11 |
CA2656139C (en) | 2012-06-05 |
EP3351100A1 (en) | 2018-07-25 |
ES2969987T3 (es) | 2024-05-23 |
JP2012152217A (ja) | 2012-08-16 |
JP2014140380A (ja) | 2014-08-07 |
JP5925826B2 (ja) | 2016-05-25 |
EP2038402A2 (en) | 2009-03-25 |
WO2007149847A3 (en) | 2008-08-14 |
BRPI0713683A2 (pt) | 2012-10-30 |
US7939316B2 (en) | 2011-05-10 |
WO2007149847A2 (en) | 2007-12-27 |
EP2038402B1 (en) | 2018-01-17 |
JP2009540833A (ja) | 2009-11-26 |
CA2656139A1 (en) | 2007-12-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5925826B2 (ja) | 細胞の凍結保存のための装置及び方法 | |
US10271543B2 (en) | Systems and methods for cryopreservation of cells | |
US9877475B2 (en) | Systems and methods for cryopreservation of cells | |
US5863715A (en) | Methods for bulk cryopreservation encapsulated islets | |
US20060019233A1 (en) | Delivery of high cell mass in a syringe and related methods of cryopreserving cells | |
CN102160546B (zh) | 冷冻保存细胞的***和方法 | |
JP6667903B2 (ja) | 中空糸凍結保存用具及び細胞凍結保存方法 | |
WO2016040063A1 (en) | Cryopreservation instrument and method of using same | |
CA2647664C (en) | Systems and methods for cryopreservation of cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110920 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20111219 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20111227 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120309 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20121127 |
|
A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20121217 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20121217 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20121226 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20160201 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5186676 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |