JP4923065B2 - 蛍光標識生物学的成分の検出のための試料採取装置及び方法 - Google Patents
蛍光標識生物学的成分の検出のための試料採取装置及び方法 Download PDFInfo
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Description
Claims (25)
- 液体試料中の生物学的成分の検出のための試料採取装置であって、
液体試料を受け取るための測定キャビティであって、所定の固定厚さ、及び撮像される面積を有し、前記試料の体積は前記測定キャビティの厚さ、及び前記撮像される面積によって定義され、それにより前記試料の単位体積当たりの生物学的成分の数を得ることができる測定キャビティと、
液体試料を採取するように構成される試料入口であって、前記測定キャビティは、前記試料入口と連絡して構成される、試料入口と、
前記測定キャビティ内部に乾燥形態で配備される試薬であって、その成分として、生物学的成分に結合するよう構成された蛍光体結合分子を含む試薬とを備え、
前記生物学的成分に結合するよう構成された前記測定キャビティ内部の全ての前記試薬成分は、前記液体試料中に溶解可能、又は縣濁可能である、試料採取装置。 - 前記蛍光体結合分子が、生物学的成分の特定の分子構造に結合するように構成される請求項1に記載の試料採取装置。
- 前記測定キャビティを画定する2つの平坦な面を有する本体部材を備える請求項1に記載の試料採取装置。
- 前記平坦な面が、光学的測定のための試料厚さを決定するために互いから所定の距離に配置される請求項3に記載の試料採取装置。
- 前記測定キャビティが、50〜170マイクロメートルの均一な厚さを有する請求項1に記載の試料採取装置。
- 前記測定キャビティが、少なくとも100マイクロメートルの均一な厚さを有する請求項5に記載の試料採取装置。
- 前記測定キャビティが、150マイクロメートル以下の均一な厚さを有する請求項5に記載の試料採取装置。
- さらに、前記測定キャビティを試料採取装置の外部と連絡する試料入口を備え、前記入口が、液体試料を採取するように構成される請求項1に記載の試料採取装置。
- 前記入口が、毛細管力によって液体試料を採取するように構成される請求項8に記載の試料採取装置。
- 前記試薬が、前記試薬を乾燥形態で残すように蒸発する揮発性液体中に溶解されて、表面に塗布される請求項1に記載の試料採取装置。
- 前記蛍光体結合分子が、抗体又は抗体フラグメントである請求項1に記載の試料採取装置。
- 使い捨てである請求項1に記載の試料採取装置。
- 液体試料中の蛍光体標識生物学的成分の検出のための方法であって、
試料採取装置の測定キャビティ内に前記液体試料を導入する段階であって、前記測定キャビティが所定の固定厚さ、及び撮像される面積を有し、前記試料の体積は前記測定キャビティの厚さ、及び前記撮像される面積によって定義され、前記試料採取装置は前記測定キャビティ内部に乾燥形態で配備され、蛍光体結合分子を有する試薬を含む段階と、
蛍光体結合分子が液体試料中の生物学的成分の特定の分子構造に結合するように、蛍光体結合分子を含む試薬を液体試料と混合する段階と、
前記測定キャビティ内の前記試料が撮像されるようにされた領域を、前記蛍光体の励起波長に対応する波長の電磁放射で照射する段階と、
前記測定キャビティの厚さ全体中の蛍光体標識生物学的成分を検出する段階であって、前記測定キャビティ内の前記照射された領域のデジタル画像を取得することを含む段階と、
前記蛍光体を示す生物学的成分を識別するためにデジタル画像をデジタルに分析する段階と
前記試料内の蛍光体を示す生物学的成分の数を決定する段階と、
を含み
前記蛍光体を示す生物学的成分が、前記デジタル画像内で、前記蛍光体の放出波長に対応する波長の電磁放射を放出する蛍光ドットとして判別される方法。 - 前記混合が、前記試薬に接触するように前記液体試料を前記測定キャビティ内に導入することによって実現される請求項13に記載の方法。
- 前記デジタル画像が、3〜50倍の光学倍率を使用して取得される請求項13に記載の方法。
- 前記デジタル画像が、3〜10倍の光学倍率を使用して取得される請求項13に記載の方法。
- 前記解析する段階が、放出される電磁放射によって生じる前記デジタル画像の領域を識別する段階を含む請求項13に記載の方法。
- 前記解析する段階が、放出される電磁放射によって生じる前記デジタル画像内のドットを識別する段階を含む請求項13に記載の方法。
- 前記解析する段階が、2つ以上の得られた画像を重ね合わせる段階を含み、各画像が、それぞれ特定の放出波長を表す請求項13に記載の方法。
- 前記解析する段階が、前記取得されたデジタル画像を電子的に拡大する段階を含む請求項13に記載の方法。
- 前記液体試料が、毛細管力によって、毛細管試料入口を通して前記試料採取装置の前記測定キャビティ内に導入される請求項13に記載の方法。
- 前記デジタル画像が、少なくとも前記測定キャビティの厚さに対応する被写界深度で取得される請求項13に記載の方法。
- 前記分析される液体試料の体積が、前記測定キャビティの厚さと、撮像される前記試料の面積とによって正確に定義される請求項13に記載の方法。
- 前記照射が、発光ダイオードを備える光源によって行われる請求項13に記載の方法。
- 励起波長に対応する前記波長が、色フィルタと組み合わせて発光ダイオードを使用することによって実現される請求項13に記載の方法。
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KR101055544B1 (ko) | 2011-08-08 |
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WO2007111555A1 (en) | 2007-10-04 |
CN101410708B (zh) | 2012-01-18 |
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CA2637592A1 (en) | 2007-10-04 |
AU2007229975A1 (en) | 2007-10-04 |
AU2007229975B2 (en) | 2010-10-28 |
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JP2009527734A (ja) | 2009-07-30 |
ZA200805865B (en) | 2009-11-25 |
SE531233C2 (sv) | 2009-01-27 |
KR20080098657A (ko) | 2008-11-11 |
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