JP4297148B2 - 核酸回収装置及び核酸回収方法 - Google Patents
核酸回収装置及び核酸回収方法 Download PDFInfo
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- JP4297148B2 JP4297148B2 JP2006257791A JP2006257791A JP4297148B2 JP 4297148 B2 JP4297148 B2 JP 4297148B2 JP 2006257791 A JP2006257791 A JP 2006257791A JP 2006257791 A JP2006257791 A JP 2006257791A JP 4297148 B2 JP4297148 B2 JP 4297148B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
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Description
本発明に係る核酸回収装置が有する微小流路の例について、以下説明する。
本発明に係る核酸回収方法の例について、以下説明する。
Claims (8)
- カオトロピックイオンで処理された生物試料からトータルRNAを回収するための核酸回収装置であって、細孔径が10〜12nm、平均粒径が10μm以下のシリカマイクロビーズによる集積部分が流路内に形成された微小流路を有する核酸回収装置。
- 前記流路の集積部分における内径が0.32〜1.00mmであることを特徴とする請求項1記載の核酸回収装置。
- 前記シリカマイクロビーズは、粒径5μmの場合の比表面積が320m2/g以上であることを特徴とする請求項1記載の核酸回収装置。
- 生物試料をDNAチップに供給する核酸供給装置として用いられる請求項1記載の核酸回収装置。
- 細孔径が10〜12nm、平均粒径が10μm以下のシリカマイクロビーズによる集積部分が流路内に形成された微小流路に、カオトロピックイオンで処理したトータルRNA溶液を通過させ、前記シリカマイクロビーズにトータルRNAを吸着させる手順を少なくとも含む、核酸回収方法。
- 前記集積部分における微小流路の内径が0.32〜1.00mmであることを特徴とする請求項5記載の核酸回収方法。
- 前記シリカマイクロビーズは、粒径5μmの場合の比表面積が320m2/g以上であることを特徴とする請求項5記載の核酸回収方法。
- 前処理として、試料のフィルタリングを行う手順を含むことを特徴とする請求項5記載の核酸回収方法。
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006257791A JP4297148B2 (ja) | 2006-09-22 | 2006-09-22 | 核酸回収装置及び核酸回収方法 |
EP07016929.7A EP1903110B1 (en) | 2006-09-22 | 2007-08-29 | Device for recovering nucleic acid and method for recovering nucleic acid |
KR1020070089057A KR101423635B1 (ko) | 2006-09-22 | 2007-09-03 | 미소 유로, 핵산 회수 장치 및 핵산 회수 방법 |
CN2007101521307A CN101153264B (zh) | 2006-09-22 | 2007-09-14 | 微通道、用于回收核酸的装置和回收核酸的方法 |
US11/858,595 US8691559B2 (en) | 2006-09-22 | 2007-09-20 | Micro channel, device for recovering nucleic acid and method for recovering nucleic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006257791A JP4297148B2 (ja) | 2006-09-22 | 2006-09-22 | 核酸回収装置及び核酸回収方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2008072987A JP2008072987A (ja) | 2008-04-03 |
JP4297148B2 true JP4297148B2 (ja) | 2009-07-15 |
Family
ID=38982955
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006257791A Expired - Fee Related JP4297148B2 (ja) | 2006-09-22 | 2006-09-22 | 核酸回収装置及び核酸回収方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8691559B2 (ja) |
EP (1) | EP1903110B1 (ja) |
JP (1) | JP4297148B2 (ja) |
KR (1) | KR101423635B1 (ja) |
CN (1) | CN101153264B (ja) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4904973B2 (ja) * | 2005-08-08 | 2012-03-28 | ソニー株式会社 | 核酸回収方法、核酸回収装置、並びに核酸供給装置 |
US8735103B2 (en) * | 2006-12-05 | 2014-05-27 | Electronics And Telecommunications Research Institute | Natural convection-driven PCR apparatus and method using disposable polymer chip |
US8664377B2 (en) | 2009-09-30 | 2014-03-04 | Samsung Electronics Co., Ltd. | Method and apparatus for isolating nucleic acids |
CN102242053B (zh) * | 2011-04-01 | 2014-06-04 | 沈越 | 一种具有高分子三维纳米结构的生物芯片 |
US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
WO2019035335A1 (ja) * | 2017-08-18 | 2019-02-21 | Agc株式会社 | 核酸の回収方法、核酸結合用担体および核酸回収キット |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
US6426126B1 (en) * | 1997-12-19 | 2002-07-30 | Amt Holdings, Inc. | Preparation of metal coatings |
JP4907030B2 (ja) | 2000-03-24 | 2012-03-28 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 分子の単離のための多孔性強磁性又はフェリ磁性ガラス粒子 |
US20050089850A1 (en) * | 2000-10-02 | 2005-04-28 | Jeffrey Van Ness | Genotyping by liquid chromatographic analysis of short nucleic acid fragments |
JP2002209580A (ja) | 2001-01-15 | 2002-07-30 | Jsr Corp | 核酸の分離方法および球状担体 |
US7208271B2 (en) | 2001-11-28 | 2007-04-24 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
US20040091411A1 (en) * | 2002-11-08 | 2004-05-13 | Bijan Modrek-Najafabadi | High surface area, high porosity silica packing with narrow particle and pore diameter distribution and methods of making same |
JP5162095B2 (ja) * | 2003-03-24 | 2013-03-13 | ソニー株式会社 | マイクロチップ、核酸抽出用のキット及び核酸の抽出方法 |
JP2005110503A (ja) | 2003-10-02 | 2005-04-28 | Arkray Inc | 核酸の精製方法及びデバイス |
JP4441610B2 (ja) | 2004-05-31 | 2010-03-31 | 独立行政法人産業技術総合研究所 | 核酸の単離方法 |
JP4626350B2 (ja) * | 2005-03-22 | 2011-02-09 | ソニー株式会社 | ハイブリダイゼーション検出に適する反応部などを有する流路系、該流路系を用いるハイブリダイゼーション検出装置 |
JP4904973B2 (ja) | 2005-08-08 | 2012-03-28 | ソニー株式会社 | 核酸回収方法、核酸回収装置、並びに核酸供給装置 |
US20070084774A1 (en) * | 2005-10-19 | 2007-04-19 | Agilent Technologies, Inc. | Chromatographic stationary phase |
US20070090034A1 (en) * | 2005-10-20 | 2007-04-26 | Agilent Technologies, Inc. | Substrate for a chromatography column |
JP4940756B2 (ja) * | 2006-05-22 | 2012-05-30 | ソニー株式会社 | マイクロ流路系 |
-
2006
- 2006-09-22 JP JP2006257791A patent/JP4297148B2/ja not_active Expired - Fee Related
-
2007
- 2007-08-29 EP EP07016929.7A patent/EP1903110B1/en not_active Expired - Fee Related
- 2007-09-03 KR KR1020070089057A patent/KR101423635B1/ko active IP Right Grant
- 2007-09-14 CN CN2007101521307A patent/CN101153264B/zh not_active Expired - Fee Related
- 2007-09-20 US US11/858,595 patent/US8691559B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN101153264A (zh) | 2008-04-02 |
EP1903110A3 (en) | 2008-04-02 |
CN101153264B (zh) | 2013-03-27 |
EP1903110B1 (en) | 2014-11-26 |
US8691559B2 (en) | 2014-04-08 |
KR101423635B1 (ko) | 2014-07-25 |
US20080161553A1 (en) | 2008-07-03 |
JP2008072987A (ja) | 2008-04-03 |
EP1903110A2 (en) | 2008-03-26 |
KR20080027134A (ko) | 2008-03-26 |
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