JP2018535449A - 試料区画化要素、顕微鏡検査方法、及び顕微鏡 - Google Patents
試料区画化要素、顕微鏡検査方法、及び顕微鏡 Download PDFInfo
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Abstract
Description
有利な構成は、従属請求項において提供されている。
Varianz)が±10%以下であると有利である。小さい分散は、実効屈折率neffのより正確な調整を可能にし、実効屈折率neffの局所的な不均一性が低減される。
Probenbegrenzungselements)が有利に実現される。
Handbook, Vol. 4, Ceramic and Glasses: 427 - 432)、において開示されている多孔質材料の少なくとも1つから成ることができる。
試料3は、照射対物レンズ25によって照射される、又は、照射されることができる。
1.1 試料側表面
1.2 対物レンズ側表面
1.3 シリンダ長手軸
2 孔
3 試料
4 浸漬媒体
5 浸漬対物レンズ
6 顕微鏡
6.1 光軸
7 対物担体
8 対物担体ホルダ
9 観察ビーム
10 焦点面
11 孔勾配
12 孔の無い層
13 シーリング層
14 媒体
15 埋設媒体
16 気泡
17 マニピュレータ
18 浸漬媒体交換装置
19 ポンプユニット
20 浸漬媒体供給部
21 制御ユニット
22 検出ユニット
23 光源
24 ドライブ
25 照明対物レンズ
d 厚さ
Claims (11)
- 少なくとも1つの領域において、顕微鏡によって捕捉可能な少なくとも1つの観察ビームに対して透明なナノ多孔質材料から成る、試料区画化要素であって、
前記ナノ多孔質材料は、平均孔径が前記観察ビームの波長よりも小さい孔を有し、
前記ナノ多孔質材料の少なくとも部分において、前記孔によって、前記ナノ多孔質材料の体積の少なくとも5%の比率が占められ、
前記ナノ多孔質材料は、開口多孔性を有し、
前記平均孔径は最高1000nmであり、
前記ナノ多孔質材料の単位体積当たりの前記孔の数、及び/又は、前記平均孔径は、前記試料区画化要素の少なくとも1つの広がりに沿って変化し、従って前記試料区画化要素において孔勾配が形成され、
前記孔勾配は、多孔性を有しない少なくとも1つの勾配部分を備え、従って、前記試料区画化要素を用いて、前記試料区画化要素の表面上の試料媒体と、前記試料区画化要素の反対側の表面上の浸漬媒体と、が分離される、
試料区画化要素。 - 前記平均孔径は0.5nm〜100nmの値範囲にある、
請求項1記載の試料区画化要素。 - 前記平均孔径は1nm〜10nmの値範囲にある、
請求項1記載の試料区画化要素。 - 前記観察ビームの波長は、200〜2000nmの波長範囲から選択される、
請求項1乃至3いずれか1項記載の試料区画化要素。 - 前記試料区画化要素の2つの相対向する前記表面から出発するそれぞれ1つの孔勾配が構成され、
前記孔勾配は前記孔の数に関して前記材料の及び/又は平均孔径の単位体積ごとに相互に反対向きに構成される、
請求項4記載の試料区画化要素。 - 既知の光学特性を有する硬化又は液状媒体によって前記孔を充填し、
前記硬化又は液状媒体は前記試料区画化要素内に留まる、
請求項1乃至5いずれか1項記載の試料区画化要素。 - 前記ナノ多孔質材料は、ガラス、プラスチック、又はプラスチック混合体である、
請求項1乃至6いずれか1項記載の試料区画化要素。 - 請求項1乃至5いずれか1項記載の試料区画化要素を準備するステップと、
試料を準備するステップと、
前記試料を前記試料区画化要素で覆うステップと、
請求項1乃至5いずれか1項気記載の前記試料区画化要素の前記孔を浸漬媒体及び/又は試料媒体で充填するステップと、
前記試料区画化要素を浸漬媒体で濡らすステップと、
浸漬対物レンズを前記浸漬媒体と接触させるステップと、
前記観察ビームの少なくとも1つの光線が前記浸漬対物レンズを介して、かつ前記試料区画化要素を介して検出されることにより、前記試料を観察するステップと、
を含む、顕微鏡検査方法。 - 試料として埋設媒体に埋設された試料が準備される、
請求項8記載の顕微鏡検査方法。 - 請求項8記載の方法を実行するための顕微鏡であって、
対物担体を保持するための対物担体ホルダを備え、
請求項1乃至7いずれか1項記載の試料区画化要素が設けられた対物担体が存在することを特徴とする、
顕微鏡。 - 請求項1乃至7いずれか1項記載の試料区画化要素に浸漬媒体を供給するための浸漬媒体供給部と、
前記浸漬媒体を、前記浸漬媒体供給部を介してコンテナ容器から前記試料区画化要素へ制御して移送するためのポンプユニットと、
前記ポンプユニットを駆動制御するための制御ユニットと、
を備える浸漬媒体交換装置によって特徴づけられる、
請求項10記載の顕微鏡。
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DE102015221044.8A DE102015221044A1 (de) | 2015-10-28 | 2015-10-28 | Probenbegrenzungselement, Mikroskopierverfahren und Mikroskop |
DE102015221044.8 | 2015-10-28 | ||
PCT/EP2016/074786 WO2017071978A1 (de) | 2015-10-28 | 2016-10-14 | Probenbegrenzungselement aus nanoporösem material für die immersionsmikroskopie |
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EP (1) | EP3368935B1 (ja) |
JP (1) | JP6807923B2 (ja) |
CN (1) | CN108292035B (ja) |
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WO (1) | WO2017071978A1 (ja) |
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DE102017217192A1 (de) | 2017-09-27 | 2019-03-28 | Carl Zeiss Microscopy Gmbh | Immersionsmatrix, dessen Verwendung und Immersionsvorrichtung |
DE102017217380A1 (de) * | 2017-09-29 | 2019-04-04 | Carl Zeiss Microscopy Gmbh | Immersionsvorrichtung zur dynamischen Anpassung eines Mediums an eine Probe |
DE102018214054A1 (de) * | 2018-08-21 | 2020-02-27 | Carl Zeiss Microscopy Gmbh | Verfahren zur Anpassung optischer Eigenschaften einer Immersionsmischung sowie Immersionsmischungen und deren Komponenten |
DE102018221670A1 (de) * | 2018-12-13 | 2020-06-18 | Karlsruher Institut für Technologie | Vorrichtung und Verfahren zur optischen Charakterisierung oder Bearbeitung eines Objekts |
WO2020176591A1 (en) * | 2019-02-27 | 2020-09-03 | Calico Life Sciences Llc | Oblique plane microscopy system and method |
DE102019109832B3 (de) * | 2019-04-12 | 2020-04-23 | Leica Microsystems Cms Gmbh | Lichtblattmikroskop und Verfahren zum Erfassen einer Messgröße |
DE102020111715A1 (de) * | 2020-04-29 | 2021-11-04 | Carl Zeiss Microscopy Gmbh | Immersionsobjektiv und verfahren zur immersionsmikroskopie |
CN114060004B (zh) * | 2021-11-11 | 2022-06-03 | 西南石油大学 | 一种基于微观驱替实验玻璃夹持模型及实验方法 |
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US20180321480A1 (en) | 2018-11-08 |
WO2017071978A1 (de) | 2017-05-04 |
CN108292035B (zh) | 2021-07-27 |
CN108292035A (zh) | 2018-07-17 |
EP3368935A1 (de) | 2018-09-05 |
EP3368935B1 (de) | 2022-02-02 |
US11280992B2 (en) | 2022-03-22 |
JP6807923B2 (ja) | 2021-01-06 |
DE102015221044A1 (de) | 2017-05-04 |
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