JP2003530859A5 - - Google Patents

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Publication number
JP2003530859A5
JP2003530859A5 JP2001577511A JP2001577511A JP2003530859A5 JP 2003530859 A5 JP2003530859 A5 JP 2003530859A5 JP 2001577511 A JP2001577511 A JP 2001577511A JP 2001577511 A JP2001577511 A JP 2001577511A JP 2003530859 A5 JP2003530859 A5 JP 2003530859A5
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JP
Japan
Prior art keywords
reaction mixture
bacteriophage
sample
target
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP2001577511A
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English (en)
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JP2003530859A (ja
Filing date
Publication date
Priority claimed from US09/548,157 external-priority patent/US6436661B1/en
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Publication of JP2003530859A publication Critical patent/JP2003530859A/ja
Publication of JP2003530859A5 publication Critical patent/JP2003530859A5/ja
Withdrawn legal-status Critical Current

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【特許請求の範囲】
【請求項１】 標的バクテリオファージを検出する方法であって、細菌を当該サンプルと組み合わせて反応混合物を形成する工程と、
当該サンプル中に存在する任意の標的バクテリオファージが細菌を溶解して酵素を放出させるのに効果的な条件下で反応混合物を培養する工程と、
固定化酵素基質を反応混合物に添加する工程と、
固定化酵素基質と任意の存在する放出された酵素との相互作用から生じる検出可能な信号について反応混合物をモニターする工程と、
を含む方法。
【請求項２】 標的細菌を検出する方法であって、バクテリオファージを当該サンプルと組み合わせて反応混合物を形成する工程と、
バクテリオファージが当該サンプル中に存在する任意の標的細菌を溶解して酵素を放出させるのに効果的な条件下で反応混合物を培養する工程と、
固定化酵素基質を反応混合物に添加する工程と、
固定化酵素基質と任意の存在する放出された酵素との相互作用から生じる検出可能な信号について反応混合物をモニターする工程と、
を含む方法。
【請求項３】 標的細菌を検出する方法であって、バクテリオファージを当該サンプルと組み合わせて反応混合物を形成する工程と、
バクテリオファージを当該サンプル中に存在する任意の標的細菌に感染させる工程と、
抗ウイルス剤を添加して任意の細胞外バクテリオファージを不活性化する工程と、
細菌ヘルパー細胞を反応混合物に添加する工程と、
固定化酵素基質を反応混合物に添加する工程と、
バクテリオファージが存在する任意の標的細菌と、細菌ヘルパー細胞とを溶解して酵素を放出させるのに効果的な条件下で反応混合物を培養する工程と、
固定化酵素基質と存在する任意の放出された酵素との相互作用から生じる検出可能な信号について反応混合物をモニターする工程と、
を含む方法。
JP2001577511A 2000-04-13 2000-08-14 固定化酵素基材を使用した細菌およびバクテリオファージの検出 Withdrawn JP2003530859A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/548,157 2000-04-13
US09/548,157 US6436661B1 (en) 2000-04-13 2000-04-13 Bacteria and bacteriophage detection using immobilized enzyme substrates
PCT/US2000/022277 WO2001079528A1 (en) 2000-04-13 2000-08-14 Bacteria and bacteriophage detection using immobilized enzyme substrates

Publications (2)

Publication Number Publication Date
JP2003530859A JP2003530859A (ja) 2003-10-21
JP2003530859A5 true JP2003530859A5 (ja) 2007-08-30

Family

ID=24187649

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001577511A Withdrawn JP2003530859A (ja) 2000-04-13 2000-08-14 固定化酵素基材を使用した細菌およびバクテリオファージの検出

Country Status (8)

Country Link
US (2) US6436661B1 (ja)
EP (1) EP1272658B1 (ja)
JP (1) JP2003530859A (ja)
AT (1) ATE263253T1 (ja)
AU (1) AU2000269056A1 (ja)
CA (1) CA2405732A1 (ja)
DE (1) DE60009575T2 (ja)
WO (1) WO2001079528A1 (ja)

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