IN2014DN09073A - - Google Patents

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Publication number
IN2014DN09073A
IN2014DN09073A IN9073DEN2014A IN2014DN09073A IN 2014DN09073 A IN2014DN09073 A IN 2014DN09073A IN 9073DEN2014 A IN9073DEN2014 A IN 9073DEN2014A IN 2014DN09073 A IN2014DN09073 A IN 2014DN09073A
Authority
IN
India
Prior art keywords
methods
disease
diagnose
practicing
toxicity
Prior art date
Application number
Inventor
Karl A Deisseroth
Kwanghun Chung
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of IN2014DN09073A publication Critical patent/IN2014DN09073A/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/549—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic with antigen or antibody entrapped within the carrier
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N2001/4038—Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures

Abstract

Methods and compositions are provided for preparing a biological specimen for microscopic analysis. These methods find many uses for example in medicine and research e.g. to diagnose or monitor disease or graft transplantation to study healthy or diseased tissue to screen candidate agents for toxicity and efficacy in disease modification. Also provided are reagents devices kits and systems thereof that find use in practicing the subject methods.
IN9073DEN2014 2012-08-09 2013-03-13 IN2014DN09073A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261681551P 2012-08-09 2012-08-09
PCT/US2013/031066 WO2014025392A1 (en) 2012-08-09 2013-03-13 Methods and compositions for preparing biological specimens for microscopic analysis

Publications (1)

Publication Number Publication Date
IN2014DN09073A true IN2014DN09073A (en) 2015-05-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
IN9073DEN2014 IN2014DN09073A (en) 2012-08-09 2013-03-13

Country Status (9)

Country Link
US (3) US10545075B2 (en)
EP (2) EP2883030B1 (en)
JP (1) JP6228205B2 (en)
KR (1) KR102148747B1 (en)
CN (1) CN104350372B (en)
AU (1) AU2013300171B2 (en)
HK (1) HK1204677A1 (en)
IN (1) IN2014DN09073A (en)
WO (1) WO2014025392A1 (en)

Families Citing this family (127)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
EP4249605A3 (en) 2011-12-22 2023-11-15 President And Fellows Of Harvard College Methods for analyte detection
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
EP2883030B1 (en) 2012-08-09 2022-08-31 The Board of Trustees of the Leland Stanford Junior University Methods and compositions for preparing biological specimens for microscopic analysis
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
DE102013205115A1 (en) * 2013-03-22 2014-09-25 Leica Microsystems Cms Gmbh SPIM arrangement
CN111662960B (en) 2013-06-25 2024-04-12 普罗格诺西斯生物科学公司 Spatially encoded bioanalytical analysis using microfluidic devices
DE102013216934A1 (en) * 2013-08-26 2015-02-26 Leica Microsystems Cms Gmbh Method and device for increasing the optical transparency of regions of a tissue sample
KR20160058900A (en) * 2013-09-20 2016-05-25 캘리포니아 인스티튜트 오브 테크놀로지 Methods for phenotyping of intact whole tissues
US10794802B2 (en) * 2013-09-20 2020-10-06 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
CN103558055B (en) * 2013-09-29 2016-01-06 中国疾病预防控制中心病毒病预防控制所 A kind of locating super-thin dicing method for infecting viral sick cell
EP3108218A4 (en) * 2014-02-21 2017-11-15 Massachusetts Institute Of Technology Expansion microscopy
WO2015154000A1 (en) 2014-04-04 2015-10-08 Massachusetts Institute Of Technology Active transport of charged molecules into, within, and/or from charged matrices
EP3149534A4 (en) 2014-05-30 2018-01-31 The Board of Trustees of The Leland Stanford Junior University Methods and devices for imaging large intact tissue samples
JP6778186B2 (en) * 2014-07-07 2020-10-28 ロゴス バイオシステムズ， インコーポレイテッド Tissue clearing device using electrophoresis
CN106715768B (en) 2014-07-30 2020-06-16 哈佛学院院长及董事 System and method for assaying nucleic acids
US10571371B2 (en) 2014-08-07 2020-02-25 Wake Forest University Health Sciences Compositions and methods for clearing a biological sample
KR101563826B1 (en) * 2014-12-31 2015-10-28 계명대학교 산학협력단 Mothod for tissue clearing
AU2016218055B2 (en) * 2015-02-09 2019-05-16 Ventana Medical Systems, Inc. Materials and methods for standardizing diffusion of a fluid into tissues
DK3901281T3 (en) 2015-04-10 2023-01-23 Spatial Transcriptomics Ab SPATIALLY SEPARATE, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SAMPLES
US11408890B2 (en) 2015-04-14 2022-08-09 Massachusetts Institute Of Technology Iterative expansion microscopy
US10526649B2 (en) 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
US10059990B2 (en) 2015-04-14 2018-08-28 Massachusetts Institute Of Technology In situ nucleic acid sequencing of expanded biological samples
CN111579769B (en) * 2015-04-30 2023-10-27 李小卫 Method for performing immune labeling on tissue sample
CN104965016A (en) * 2015-06-15 2015-10-07 南昌大学 Integrated biological tissue transparency device
EP3332029B1 (en) * 2015-08-07 2021-10-06 Massachusetts Institute of Technology Nanoscale imaging of proteins and nucleic acids via expansion microscopy
WO2017027368A1 (en) * 2015-08-07 2017-02-16 Massachusetts Institute Of Technology Protein retention expansion microscopy
WO2017031249A1 (en) * 2015-08-17 2017-02-23 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
WO2017075265A1 (en) * 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
JP2019507352A (en) * 2015-12-02 2019-03-14 クリアライト ダイアグノスティックス リミテッド ライアビリティ カンパニー Methods for the preparation and analysis of tumor tissue samples for cancer detection and monitoring
CN108431664B (en) * 2015-12-23 2021-11-02 皇家飞利浦有限公司 Fluorescent calibration slide
CN105823807B (en) * 2016-01-04 2018-04-20 浙江农林大学 Cancer therapy drug Hydroxycamptothecin Composition analyzed device and detection method
US11254974B2 (en) * 2016-02-10 2022-02-22 The Board Of Trustees Of The Leland Stanford Junior University RNA fixation and detection in clarity-based hydrogel tissue
CA3016361C (en) * 2016-03-07 2024-01-02 X-Zell Inc. Compositions and methods for identifying rare cells
JP6721899B2 (en) * 2016-03-30 2020-07-15 国立大学法人 大分大学 Embedding method for electron microscope mainly composed of highly hydrophilic polymer monomer
WO2017189525A1 (en) 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US11397140B2 (en) * 2016-04-29 2022-07-26 Massachusetts Institute Of Technology Methods for reversible and tunable tissue magnification
US10852290B2 (en) 2016-05-11 2020-12-01 Bonraybio Co., Ltd. Analysis accuracy improvement in automated testing apparatus
US10324022B2 (en) 2016-05-11 2019-06-18 Bonraybio Co., Ltd. Analysis accuracy improvement in automated testing apparatus
US10281386B2 (en) * 2016-05-11 2019-05-07 Bonraybio Co., Ltd. Automated testing apparatus
EP3252452A1 (en) 2016-05-25 2017-12-06 The Board of Trustees of the Leland Stanford Junior University Method for imaging and analysis of a biological specimen
WO2017205531A1 (en) * 2016-05-25 2017-11-30 The Board Of Trustees Of The Leland Stanford Junior University Methods for visualization and quantification of fiber-like structures
WO2017222453A1 (en) 2016-06-21 2017-12-28 Hauling Thomas Nucleic acid sequencing
CN107621462B (en) * 2016-07-13 2020-03-31 王志伟 Tissue clearing liquid SUT and preparation and application thereof
CN107674879A (en) * 2016-08-01 2018-02-09 深圳先进技术研究院 A kind of photogene plasmid and its application
WO2018044899A1 (en) 2016-08-29 2018-03-08 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method and composition for optical clearing of tissues
CN109923216A (en) 2016-08-31 2019-06-21 哈佛学院董事及会员团体 By the detection combination of biomolecule to the method for the single test using fluorescent in situ sequencing
JP7239465B2 (en) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ Methods for preparing nucleic acid sequence libraries for detection by fluorescence in situ sequencing
US10538632B1 (en) 2016-09-30 2020-01-21 National Technology & Engineering Solutions Of Sandia, Llc Shape-preserving polymeric replication of biological matter
JP6898720B2 (en) * 2016-10-14 2021-07-07 株式会社ファンケル Skin internal structure evaluation method
CN116242687A (en) * 2016-11-08 2023-06-09 哈佛学院院长及董事 Multiplex imaging using MERFISH, extended microscopy and related techniques
CN110140175A (en) * 2016-11-08 2019-08-16 哈佛学院院长及董事 Matrix trace and removing
KR101866249B1 (en) 2016-11-29 2018-06-12 박순현 Composition for clrearing of biotissue and clarity method for biotissue using thereof
DE102016123458B3 (en) 2016-12-05 2018-03-15 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin Process for the preparation of transparent biological preparations for a light microscopic examination
CN106682453B (en) * 2016-12-28 2019-03-26 长春理工大学 A kind of fixed point edit methods of DNA molecular
US10955322B2 (en) 2017-01-18 2021-03-23 California Institute Of Technology Methods and devices for soft and osseous tissue clearing and fluorescent imaging
CN106769350B (en) * 2017-01-20 2019-11-22 上海交通大学 A method of for being rich in the quick and complete transparence of fat drips tissue
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
KR101888782B1 (en) * 2017-02-07 2018-08-14 고려대학교 산학협력단 Method for clearing molecular structures in skins, method for three-dimensional imaging and method for diagnosis and evaluation using the same
US11436719B2 (en) 2017-02-16 2022-09-06 Kyoto University Cell evaluation method, cell evaluation device, and recording medium storing cell evaluation program
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
KR20180099482A (en) * 2017-02-27 2018-09-05 고려대학교 산학협력단 Method for preparing decellularized tissue using hydrogel polymer and decellularized tissue prepared therefrom
WO2018155925A2 (en) * 2017-02-27 2018-08-30 고려대학교 산학협력단 Method for producing decellularized tissue using hydrogel polymer and decellularized tissue produced thereby
TWI620927B (en) * 2017-04-21 2018-04-11 胡宇光 Method and kit for staining neural tissue sample and method for visualizing neurons
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
MX2017008919A (en) * 2017-07-05 2018-03-01 Gabriela Reyes Fuchs Carmen Process for forming a color image of incinerated materials by microscopy techniques.
JP7160350B2 (en) * 2017-07-06 2022-10-25 公立大学法人大阪 Biological tissue clearing method and its reagent
KR101996722B1 (en) * 2017-07-07 2019-07-04 재단법인대구경북과학기술원 A method of electrically moving a biological molecule using multi-channel thin membrane electrodes
WO2019023214A1 (en) 2017-07-25 2019-01-31 Massachusetts Institute Of Technology In situ atac sequencing
WO2019068880A1 (en) 2017-10-06 2019-04-11 Cartana Ab Rna templated ligation
KR102200618B1 (en) * 2018-01-12 2021-01-08 재단법인대구경북과학기술원 A composition comprising sulfobetainic zwitterionic detergents for tissue penetration of biological molecules and the use thereof
WO2019078655A1 (en) * 2017-10-19 2019-04-25 재단법인대구경북과학기술원 Composition for infiltrating biomolecule into tissue including sulfobetaine-based zwitterionic surfactant, and use thereof
US10488307B2 (en) 2017-12-12 2019-11-26 Dignity Health Systems, devices, and methods for improved tissue treatment
KR102050248B1 (en) * 2017-12-29 2019-11-29 연세대학교 산학협력단 Method for analysis of tissue clarity measurement
CN107966477A (en) * 2018-01-22 2018-04-27 哈尔滨工业大学 A kind of biosensor
WO2019156957A1 (en) 2018-02-06 2019-08-15 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
WO2020011735A2 (en) 2018-07-09 2020-01-16 Universität Zürich Systems, apparatuses, and methods for preparation of tissue samples
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC Methods and systems for sample processing or analysis
KR102151128B1 (en) * 2018-08-24 2020-09-02 주식회사 바이나리 Pressure device for enhancing of tissue clearing and antibody penetration using nitrogen gas
EP3845629B1 (en) * 2018-08-29 2023-10-25 National Institute for Materials Science Measurement device and evaluation method
KR102368585B1 (en) * 2018-09-28 2022-03-03 서울대학교산학협력단 Bioimaging technique using reversibly expandable/recoverable hydrogel dependent on temperature change
US11435302B2 (en) * 2018-11-13 2022-09-06 The Trustees Of Princeton University X-ray assisted electron microscopy staining procedure
GB201818567D0 (en) * 2018-11-14 2018-12-26 Francis Crick Institute Ltd Composition
CN109612811A (en) * 2018-12-26 2019-04-12 华中科技大学苏州脑空间信息研究院 A kind of hydrogel embedding method for protecting mechanics of biological tissue and fluorescence
CN109781493A (en) * 2018-12-26 2019-05-21 华中科技大学苏州脑空间信息研究院 It is a kind of can long-time continuous fine upper cut imaging biological tissue's hydrogel embedding method
CN110006862B (en) * 2019-03-29 2020-09-08 华中科技大学 Expansion cutting microscopic imaging method and super-absorbent hydrogel suitable for method
CN112740013A (en) * 2019-05-09 2021-04-30 科来旺科技公司 Method and apparatus for electrophoretic staining of biological samples using ion-conducting membranes
AU2020282024A1 (en) 2019-05-31 2021-11-11 10X Genomics, Inc. Method of detecting target nucleic acid molecules
WO2021095718A1 (en) * 2019-11-11 2021-05-20 公立大学法人大阪 Method for making biological tissue transparent and reagent for same
US11802822B2 (en) 2019-12-05 2023-10-31 Massachusetts Institute Of Technology Multiplexed expansion (MultiExM) pathology
US20210254140A1 (en) 2020-02-17 2021-08-19 10X Genomics, Inc. Situ analysis of chromatin interaction
US20210262018A1 (en) 2020-02-21 2021-08-26 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
CN115551638A (en) * 2020-03-13 2022-12-30 耶鲁大学 Method and system for physical expansion and imaging of biological samples
US20210348991A1 (en) * 2020-05-05 2021-11-11 Massachusetts Institute Of Technology Compositions and methods relating to reversibly compressible tissue-hydrogel hybrids
CN111397999B (en) * 2020-05-09 2021-12-03 中国科学院植物研究所 Simple method for enhancing contrast of micro-CT plant sample
CN114129780B (en) * 2020-09-03 2022-06-28 天津大学 Preparation and application of light-operated nitric oxide releasing composite hydrogel
RU2752751C1 (en) * 2020-09-28 2021-08-02 Ахмеджан Атаханович Аллаберганов Method for obtaining digital duplicates of document in form of paper information medium or image of text document
CN112284861B (en) * 2020-10-21 2023-02-17 上海市农业科学院 Fixing liquid for hypsizigus marmoreus basidiospore microscopic observation, preparation method, fixing method and application
US11333588B1 (en) * 2020-12-07 2022-05-17 Nebulum Technologies Co., Ltd. Matrix-assisted methods and compositions to prepare biological samples for super-resolution imaging
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
CN112665951B (en) * 2020-12-22 2022-11-11 中国农业科学院作物科学研究所 Embryo milk tissue embedding method and application
US20220195498A1 (en) 2020-12-23 2022-06-23 10X Genomics, Inc. Methods and compositions for analyte detection
US20220228200A1 (en) 2021-01-19 2022-07-21 10X Genomics, Inc. Methods and compositions for internally controlled in situ assays
WO2022164809A1 (en) 2021-01-26 2022-08-04 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
US20220282319A1 (en) 2021-03-03 2022-09-08 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
CN113004885A (en) * 2021-03-08 2021-06-22 光华临港工程应用技术研发(上海)有限公司 DNA nano-structure dye for expansion super-resolution imaging and application thereof
CN117396613A (en) 2021-06-01 2024-01-12 10X基因组学有限公司 Methods and compositions for analyte detection and probe resolution
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
WO2023283442A1 (en) 2021-07-09 2023-01-12 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
CN117651855A (en) 2021-07-13 2024-03-05 10X基因组学有限公司 Method for preparing polymeric substrates with controlled thickness
US20230057571A1 (en) 2021-08-03 2023-02-23 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US20230061542A1 (en) 2021-08-16 2023-03-02 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230279475A1 (en) 2022-01-21 2023-09-07 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023192616A1 (en) 2022-04-01 2023-10-05 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
WO2023196526A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods for multiplex cell analysis
WO2023204581A1 (en) * 2022-04-20 2023-10-26 동국대학교 산학협력단 Three-dimensional hydrogel composite having cancer cell killing effect and method for producing same
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
US20240026427A1 (en) 2022-05-06 2024-01-25 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
KR102582110B1 (en) * 2022-07-18 2023-09-21 (재)씨젠의료재단 Composition for clearing and image analysis of three-dimensional cell or tissue
KR20240042887A (en) * 2022-09-26 2024-04-02 연세대학교 산학협력단 Cell film for high magnification analysis of cell expansion, composition and method for preparing the same
WO2024081869A1 (en) 2022-10-14 2024-04-18 10X Genomics, Inc. Methods for analysis of biological samples

Family Cites Families (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3129158A (en) 1961-01-24 1964-04-14 Raymond Samuel Process for gel electrophoresis
US3208929A (en) 1962-09-11 1965-09-28 Robert H Raymond Apparatus for gel electrophoresis
US3346479A (en) 1964-04-09 1967-10-10 Scientific Industries Preparative separation by a combination of gel separation and electrophoresis
US3375187A (en) 1965-05-13 1968-03-26 Buchler Instr Inc Apparatus for temperature controlled preparative and analytical electrophoresis
US3576727A (en) 1968-05-23 1971-04-27 Bruce Lee Evatt Gel electrophoresis process
US3563880A (en) 1968-08-28 1971-02-16 Arthur W Anderson Gel electrophoresis unit
SE328135B (en) 1968-09-19 1970-09-07 Lkb Produkter Ab
US3616454A (en) 1969-03-13 1971-10-26 Univ New York Method of and apparatus for electrophoretic separation in a gel column
US3674678A (en) 1970-10-28 1972-07-04 Millipore Corp Electrophoretic apparatus
US3865712A (en) 1972-04-13 1975-02-11 Lkb Produkter Ab Electrode arrangement for an electrophoretical separation performed in a gel plate
US3989613A (en) * 1973-05-16 1976-11-02 The Dow Chemical Company Continuous balanced flow fixed boundary electrophoresis
US4088561A (en) 1977-06-27 1978-05-09 The United States Of America As Represented By The United States Department Of Energy Apparatus for electrophoresis separation
US4151065A (en) 1978-01-30 1979-04-24 The Regents Of The University Of California Horizontal slab gel electrophoresis
US4292161A (en) 1979-01-03 1981-09-29 Hoefer Scientific Instruments Vertical gel slab electrophoresis apparatus
FR2496889A1 (en) 1980-12-22 1982-06-25 Hours Michel MIGRATION AND REACTION TANK USED FOR ELECTROPHORESIS ANALYZES
US4339327A (en) 1981-04-23 1982-07-13 Tyler Jonathan M Miniature slab gel electrophoresis system
US4375401A (en) 1981-05-20 1983-03-01 Nicholas Catsimpoolas Electrophoresis system
EP0101859B1 (en) 1982-07-23 1987-10-14 Matthias Hediger Apparatus for preparative gel electrophoresis, in particular for separating mixtures on a gradient gel polyacryl amide column
US4588491A (en) 1984-02-27 1986-05-13 International Biotechnologies, Inc. Horizontal gel electrophoresis device
DE3580418D1 (en) * 1984-07-31 1990-12-13 Hitachi Ltd ELECTROPHORETIC SEPARATION PROCESS WITH FREE FLOW AND APPARATUS FOR THIS.
US4685025A (en) 1985-03-14 1987-08-04 Raychem Corporation Conductive polymer circuit protection devices having improved electrodes
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
JPH03167472A (en) * 1989-11-27 1991-07-19 Sumitomo Chem Co Ltd Method for dyeing embryo skeletal of experimental animal
JP3216907B2 (en) 1992-05-18 2001-10-09 株式会社日立製作所 Electronic zoom device and imaging device
JP3049537B2 (en) * 1995-02-03 2000-06-05 悌二 竹崎 Fixation support method for biopsy sample, fixation support agent and embedding cassette
WO1999036559A1 (en) 1998-01-13 1999-07-22 Julien Jean Pierre Viral vectors expressing self-polymerizing neuronal intermediate filaments and their use
US6722395B2 (en) 1998-01-13 2004-04-20 James W. Overbeck Depositing fluid specimens on substrates, resulting ordered arrays, techniques for analysis of deposited arrays
WO2000017355A2 (en) 1998-09-18 2000-03-30 Incyte Pharmaceuticals, Inc. Human cytoskeleton associated proteins
US6232092B1 (en) 1998-10-02 2001-05-15 Rogers Imaging Corporation Method for preparing biological specimens for visual analysis
US6219575B1 (en) 1998-10-23 2001-04-17 Babak Nemati Method and apparatus to enhance optical transparency of biological tissues
US6409774B1 (en) 1999-06-11 2002-06-25 Resolution Sciences Corporation Electrophoresis-assisted staining of materials
CA2426871A1 (en) 2000-10-24 2002-05-16 Oncosis Llc Method and device for selectively targeting cells within a three -dimensional specimen
AUPR378001A0 (en) * 2001-03-16 2001-04-12 Proteome Systems Ltd Protein chip
US6472216B1 (en) 2001-07-24 2002-10-29 Ann-Shyn Chiang Aqueous tissue clearing solution
EP1438976B1 (en) * 2001-09-28 2007-12-19 Biopharma Development Ltd Polyfunctional biocompatible hydrogel and method for the production thereof
DE10209609A1 (en) 2002-03-05 2003-10-02 Europ Lab Molekularbiolog Method and device for parallel analysis of bio-molecules
CA2491528A1 (en) 2002-06-28 2004-01-08 Cardio, Inc. Decellularized tissue
WO2004034890A2 (en) 2002-10-17 2004-04-29 Vacanti, Joseph, P. Biological scaffolding material
JP4361057B2 (en) * 2003-04-30 2009-11-11 モイル，ウィリアム，アール Sensors for biomolecule detection and cell classification
US7713923B2 (en) 2003-06-25 2010-05-11 Massachusetts Institute Of Technology Self-assembling peptides incorporating modifications and methods of use thereof
US8226715B2 (en) 2003-06-30 2012-07-24 Depuy Mitek, Inc. Scaffold for connective tissue repair
US7326571B2 (en) 2003-07-17 2008-02-05 Boston Scientific Scimed, Inc. Decellularized bone marrow extracellular matrix
US7660620B2 (en) 2003-09-29 2010-02-09 Medtronic, Inc. Timing techniques for magnetic resonance imaging
US7955788B2 (en) * 2003-10-30 2011-06-07 Medtronic, Inc. Bioprosthetic tissue preparation with synthetic hydrogels
WO2005062938A2 (en) 2003-12-23 2005-07-14 The Regents Of The University Of California Chemical agents and methods for optical clearing of skin and mucous membranes to improve light-based diagnostics and therapeutics
DE602005023426D1 (en) 2004-03-25 2010-10-21 California Inst Of Techn Hybridization CHAIN REACTION
GB0414825D0 (en) * 2004-07-02 2004-08-04 Biostatus Ltd Gel formulations and uses thereof
CN1256579C (en) * 2004-09-23 2006-05-17 上海交通大学 Method for producing biolgoical sample embedded block based on atomic force microscope observation
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
WO2007030012A2 (en) * 2005-09-05 2007-03-15 Stichting Voor De Technische Wetenscahappen Extracellular matrix imaging
US8163565B2 (en) 2005-12-08 2012-04-24 Cytyc Corporation Light curing fixative
EP1801573A1 (en) * 2005-12-21 2007-06-27 Boehringer Mannheim Gmbh Method and apparatus for parallel two-dimensional electrophoresis
US20070172911A1 (en) * 2006-01-13 2007-07-26 Michael Farrell Biological sample processing composition and method
CA2696562C (en) 2007-08-14 2016-02-16 Smith & Nephew Plc Formation of a scaffold from multiple smaller scaffold components
DE102007045897A1 (en) 2007-09-26 2009-04-09 Carl Zeiss Microimaging Gmbh Method for the microscopic three-dimensional imaging of a sample
WO2010014244A2 (en) 2008-07-30 2010-02-04 The Regents Of The University Of California, San Francisco Multidirectional selective plane illumination microscopy
US20100055733A1 (en) 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
WO2010030358A1 (en) 2008-09-12 2010-03-18 Quickmbed, Inc. Scaffold for tissue sample orientation
US9671400B2 (en) * 2009-02-19 2017-06-06 Dako Denmark A/S Conjugate molecules
CA2756225A1 (en) 2009-03-25 2010-09-30 Los Alamos National Security, Llc Fiber optical assembly for fluorescence spectrometry
EP2425216B1 (en) * 2009-04-27 2019-10-23 Expedeon Holdings Ltd. Programmable electrophoretic notch filter systems and methods
US8852614B2 (en) 2010-03-12 2014-10-07 The Board Of Trustees Of The Leland Stanford Junior University Hydrogels with network defects enhanced by nanoparticle incorporation
CN108061677B (en) * 2010-03-12 2021-10-29 国立研究开发法人理化学研究所 Clarifying agent for biological material and use thereof
US8867803B2 (en) 2010-04-20 2014-10-21 Eric J. Seibel Optical projection tomography microscopy (OPTM) for large specimen sizes
US8927624B2 (en) 2010-05-14 2015-01-06 Fujifilm Imaging Colorants Limited Dispersion, process for preparing a dispersion and ink jet printing ink
TWI425201B (en) 2010-10-01 2014-02-01 Nat Univ Tsing Hua Method for 3-dimensional microscopic visualization of thick biological tissues
CN102072836B (en) * 2010-11-12 2014-02-19 深圳华大基因科技有限公司 Method for concentration and desalting of biological macromolecule and kit
WO2012103343A1 (en) 2011-01-28 2012-08-02 The Board Of Trustees Of The Leland Stanford Junior University Functional targeted brain endoskeletonization
EP2703801B1 (en) 2011-04-28 2020-11-25 Riken Method for making biological material transparent
CN103562702A (en) 2011-05-20 2014-02-05 独立行政法人理化学研究所 Clarifying reagent for biological materials and use thereof
WO2013016712A2 (en) 2011-07-27 2013-01-31 The Rockefeller University Methods for fixing and detecting rna
JP5967528B2 (en) 2012-06-22 2016-08-10 国立研究開発法人理化学研究所 Biological material clarification method and biological material clarification treatment kit
DE102012211462A1 (en) 2012-07-03 2014-01-23 Carl Zeiss Microscopy Gmbh Method for preparing and performing image stacking of a sample from different orientation angles
EP2883030B1 (en) 2012-08-09 2022-08-31 The Board of Trustees of the Leland Stanford Junior University Methods and compositions for preparing biological specimens for microscopic analysis
DE102012020240A1 (en) 2012-10-12 2014-04-17 Carl Zeiss Microscopy Gmbh Microscope and method for SPIM microscopy
KR102368591B1 (en) 2013-04-30 2022-02-25 캘리포니아 인스티튜트 오브 테크놀로지 Multiplex labeling of molecules by sequential hybridization barcoding
US10267714B2 (en) 2013-08-14 2019-04-23 Riken Composition for preparing biomaterial with excellent light-transmitting property, and use thereof
DE102013216934A1 (en) 2013-08-26 2015-02-26 Leica Microsystems Cms Gmbh Method and device for increasing the optical transparency of regions of a tissue sample
KR20160058900A (en) 2013-09-20 2016-05-25 캘리포니아 인스티튜트 오브 테크놀로지 Methods for phenotyping of intact whole tissues
CN103513411B (en) 2013-09-27 2016-02-03 香港应用科技研究院有限公司 The apparatus and method focused in fluorescent microscope
EP3149534A4 (en) 2014-05-30 2018-01-31 The Board of Trustees of The Leland Stanford Junior University Methods and devices for imaging large intact tissue samples
US10571371B2 (en) 2014-08-07 2020-02-25 Wake Forest University Health Sciences Compositions and methods for clearing a biological sample
WO2016073941A1 (en) 2014-11-07 2016-05-12 California Institute Of Technology Methods for phenotyping of intact bones by tissue clearing and staining
JP6687949B2 (en) 2015-01-20 2020-04-28 国立研究開発法人理化学研究所 Clearing reagent for biological materials, system and use thereof
WO2016147812A1 (en) 2015-03-18 2016-09-22 国立研究開発法人理化学研究所 Method for observing biological material and clearing method
JP2019507352A (en) 2015-12-02 2019-03-14 クリアライト ダイアグノスティックス リミテッド ライアビリティ カンパニー Methods for the preparation and analysis of tumor tissue samples for cancer detection and monitoring

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