CN104965016A - Integrated biological tissue transparency device - Google Patents
Integrated biological tissue transparency device Download PDFInfo
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- CN104965016A CN104965016A CN201510328214.6A CN201510328214A CN104965016A CN 104965016 A CN104965016 A CN 104965016A CN 201510328214 A CN201510328214 A CN 201510328214A CN 104965016 A CN104965016 A CN 104965016A
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- biological tissue
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- electrophoresis tank
- transparent
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Abstract
The invention relates to an integrated biological tissue transparency device. A power source, an electrophoresis tank, a temperature control device, a singlechip, a display panel, an operation button, a water storage tank and a pump for tissue transparency are integrated into a system. Voltage strength of electrophoresis, set electrophoresis temperature, set working time and the like are controlled automatically. The biological tissue transparency device is simple and intelligent; and the work efficiency of biological tissue transparency can be improved.
Description
Technical field
The present invention relates to a kind of integrated apparatus for transparent organism tissue, belong to biological assay devices technical field.
Background technology
Senile Mouse is the field used in bio-science research widely.But, traditional Senile Mouse method be all tissue is fixing after, take the method for cutting into slices to carry out the change of research organization and cellular level.Want in integral level to study cell or to organize the change of level, this is a very difficult thing.On the one hand, histotomy is a very loaded down with trivial details process; On the other hand, the three-dimensional of tissue builds also is difficult completely.Up-to-date research shows, utilizes one to be called CLARITY(transparent) technology, can, by itself opaque tissue in animal body, after the phospholipid molecule on all cells film is all changed by liposome conversion, make tissue become optical clear.But we are in the process putting into practice this transparent technology, find the method complex steps, there is no ready-made integrated apparatus in the market.In order to improve the conventional efficient of this technology, we have developed biological tissue's transparent unit of integration.
Summary of the invention
The object of the present invention is to provide a kind of biological tissue's transparent unit of integration, the transparent experiment of easy biological tissue, improve conventional efficient.
The present invention adopts following technical proposals to realize: a kind of biological tissue's transparent unit of integration, comprises power supply, electrophoresis tank, attemperating unit, single-chip microcomputer, display panel, action button, reserve tank, pump; Electrophoresis tank, pump, reserve tank three form the circulation system, power supply is powered to electrophoresis tank, attemperating unit, single-chip microcomputer, display panel, pump, attemperating unit is arranged in reserve tank, and single-chip microcomputer is connected with power supply, attemperating unit, display panel, action button by I/O interface.
Described power supply is an ac-dc converter device, and the direct current of one group of adjustable voltage is for electrophoresis tank work, and the direct current of many group fixed voltages is for attemperating unit, single-chip microcomputer, display panel and pump work.
Described electrophoresis tank is the transparent main place of biological tissue, is the square cavity that a top is added a cover, posts positive and negative electrode in cavity.
Described attemperating unit is one group and is immersed in and adds hot-charging device and refrigeratory in reserve tank.
Described single-chip microcomputer is the control center of this transparency of organization device, is connected with power supply, attemperating unit, display panel, action button by I/O interface.Be connected with power supply the control realized electrophoresis tank operating voltage; Be connected with attemperating unit and can control the temperature of liquid in reserve tank; Connect to display panel and can show relevant real time data; Be connected with action button the design that can realize correlation parameter.
Described display panel can show voltage in electrophoresis tank, strength of current, the design temperature of display attemperating unit, display reserve tank observed temperature, and shows working time and setting working time.
Described action button is used to voltage and the strength of current of adjusting electrophoresis tank, regulates the design temperature of attemperating unit, and sets real time and the working routine of whole system.
Described reserve tank is collect the cavity overflowing liquid in electrophoresis tank, and described pump liquid is drawn into from reserve tank the propulsion system injecting electrophoresis tank, one group of fixed voltage that required power is provided by power supply.
The present invention is a kind of integrated apparatus being used for transparent organism tissue, and it is integrated with power supply, electrophoresis tank, attemperating unit, single-chip microcomputer, display panel, action button, reserve tank, pump.Utilize this integrated apparatus, as long as will treat that transparent biological tissue samples directly puts into electrophoresis tank, setting correlation parameter just can realize transparent effect quickly and easily.
Accompanying drawing explanation
Fig. 1 is single chip circuit schematic diagram of the present invention.
Fig. 2 is organization framework figure of the present invention.
Icon illustrates: 101. power supplys, 102. electrophoresis tanks, 103. attemperating units, 104. single-chip microcomputers, 105. display panels, 106. action buttons, 201. reserve tanks, 202. pumps.
Embodiment
Below in conjunction with accompanying drawing, by embodiment, the present invention is described further.
Embodiment:
This system is an integral biological transparency of organization device being integrated with power supply (101), electrophoresis tank (102), attemperating unit (103), single-chip microcomputer (104), display panel (105), action button (106), reserve tank (201), pump (202).
See Fig. 1, the control center that described single-chip microcomputer (104) is this transparent unit, is connected with power supply (101), electrophoresis tank (102), attemperating unit (103), display panel (105), action button (106) by I/O interface.Power supply (101) is to electrophoresis tank (102), attemperating unit (103), single-chip microcomputer (104), display panel (105), pump (202) power supply.Display panel (105) can show operating voltage and electric current, the observed temperature of electrophoresis liquid and the working time of single unit system of electrophoresis tank (102) in real time.Action button (106) can set the voltage of electrophoresis tank (102) and electric current, the design temperature of electrophoresis liquid and the working routine of single unit system.
See Fig. 2, described electrophoresis tank (102) is the transparent main place of biological tissue, and post positive and negative electrode in cavity, biological sample is placed between positive and negative electrode.Described pump (202) electrophoretic buffer is drawn into from reserve tank (201) propulsion system injecting electrophoresis tank (102).Described reserve tank (201) is collect the cavity overflowing liquid from electrophoresis tank (102).In Fig. 2 shown in arrow is that liquid is at reserve tank (201), the schematic diagram completing a circulation between pump (202) and electrophoresis tank (102).
Described power supply (101) is an ac-dc converter device, the direct current of one group of adjustable voltage is for electrophoresis tank (102) work, and the direct current of many group fixed voltages is for the work of attemperating unit (103), single-chip microcomputer (104), display panel (106) and pump (202).
Described electrophoresis tank (102) is the transparent main place of biological tissue, is the square cavity that a top is added a cover, posts positive and negative electrode in cavity.Electrophoresis liquid enters from the one end of electrophoresis tank (102), enters reserve tank (201) in the mode of overflowing from the bottom of cavity.Electric power needed for electrophoresis tank (102) is the one group of adjustable voltage provided by power supply (101).
Described attemperating unit (103) is one group and is immersed in and adds hot-charging device and refrigeratory in reserve tank, compare the difference in reserve tank (104) between observed temperature and preset temperature by single-chip microcomputer (104), carry out the temperature that control heater or refrigerator carry out liquid in stable water storage.Electric power needed for attemperating unit is the one group of fixed voltage provided by power supply (101).
The control center that described single-chip microcomputer (104) is this transparency of organization device, is connected with power supply (101), attemperating unit (103), display panel (105), action button (106) by I/O interface.Be connected with power supply (101) control realized electrophoresis tank (102) operating voltage; Be connected with attemperating unit (103) and the temperature of liquid in reserve tank (201) can be controlled; Connect to display panel (105) and relevant real time data can be shown; Be connected with action button (106) design that can realize correlation parameter.
Described display panel (105), can show electrophoresis tank (102) interior voltage, strength of current, the design temperature of display attemperating unit (103), display reserve tank (201) observed temperature, and shows working time and setting working time.
Described action button (106), is used to voltage and the strength of current of adjusting electrophoresis tank (102), regulates the design temperature of attemperating unit (103), and sets real time and the working routine of whole system.
Described reserve tank (201) is collect the cavity overflowing liquid in electrophoresis tank (102).Described pump (202) liquid is drawn into from reserve tank (201) propulsion system injecting electrophoresis tank (102), one group of fixed voltage that required power is provided by power supply (101).
We are for the brain of transparent adult mice, are put into by this tissue this " biological tissue's transparent unit of integration ", conveniently can realize the optical clear of this tissue.With reference to the preparation method of biological tissue's gel in citing document, first the brain of adult mice is carried out gel.Afterwards the biological tissue after gel is put in electrophoresis tank (102), the electrophoretic buffer described in list of references is added in reserve tank (201), upper in action button (105): the voltage in setting electrophoresis tank (102) is 50V, electric current is 3A, setting attemperating unit (103) maintains the temperature of 50 DEG C, and the working time is set as 36 hours.After starter gear 36 hours, the transparent effect of this biological tissue completes substantially.The blank sheet of paper that at this time one be have printed word is placed on the below of this tissue, and we can see word clearly through this tissue, illustrate that this biological sample has good optical transparent properties.
This device is integrated biological tissue's transparent unit, power supply (101) needed for transparency of organization, electrophoresis tank (102), attemperating unit (103), reserve tank (201), pump (202) are all incorporated in a device by it, control various piece achieve the transparent simplification of biological tissue with intelligent by single-chip microcomputer (104).Utilize this device, greatly can improve the transparent efficiency of biological tissue, this has important facilitation by biology this Technique Popularizing transparent.
Claims (10)
1. biological tissue's transparent unit of integration, comprise power supply, electrophoresis tank, attemperating unit, single-chip microcomputer, display panel, action button, reserve tank, pump, it is characterized in that: electrophoresis tank, pump, reserve tank three form the circulation system, power supply is powered to electrophoresis tank, attemperating unit, single-chip microcomputer, display panel, pump, attemperating unit is arranged in reserve tank, and single-chip microcomputer is connected with power supply, attemperating unit, display panel, action button by I/O interface.
2. biological tissue's transparent unit of integration according to claim 1, it is characterized in that: described power supply is an ac-dc converter device, the direct current of one group of adjustable voltage is for electrophoresis tank work, and the direct current of many group fixed voltages is for attemperating unit, single-chip microcomputer, display panel and pump work.
3. biological tissue's transparent unit of integration according to claim 1, is characterized in that: described electrophoresis tank is the transparent main place of biological tissue, is the square cavity that a top is added a cover, posts positive and negative electrode in cavity.
4. biological tissue's transparent unit of integration according to claim 1, is characterized in that: described attemperating unit is one group and is immersed in and adds hot-charging device and refrigeratory in reserve tank.
5. biological tissue's transparent unit of integration according to claim 1 or 2 or 3, is characterized in that: described single-chip microcomputer is connected with power supply the control realized electrophoresis tank operating voltage.
6. biological tissue's transparent unit of integration according to claim 1, is characterized in that: described single-chip microcomputer is connected with action button the design that can realize correlation parameter.
7. biological tissue's transparent unit of integration according to claim 1, it is characterized in that: described display panel can show voltage in electrophoresis tank, strength of current, the design temperature of display attemperating unit, display reserve tank observed temperature, and show working time and setting working time.
8. biological tissue's transparent unit of integration according to claim 1, it is characterized in that: described action button is used to adjust voltage and the strength of current of electrophoresis tank, regulate the design temperature of attemperating unit, and set real time and the working routine of whole system.
9. biological tissue's transparent unit of integration according to claim 1, is characterized in that: described reserve tank is collect the cavity overflowing liquid in electrophoresis tank.
10. biological tissue's transparent unit of integration according to claim 1 or 2, is characterized in that: described pump liquid is drawn into from reserve tank the propulsion system injecting electrophoresis tank.
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| CN201510328214.6A CN104965016A (en) | 2015-06-15 | 2015-06-15 | Integrated biological tissue transparency device |
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| CN201510328214.6A CN104965016A (en) | 2015-06-15 | 2015-06-15 | Integrated biological tissue transparency device |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004011144A2 (en) * | 2002-07-29 | 2004-02-05 | Dumas David P | Transparent polymer support for electrophoresis and electrochromatography and related methods |
| US20120064136A1 (en) * | 2010-09-10 | 2012-03-15 | Nanobio Corporation | Anti-aging and wrinkle treatment methods using nanoemulsion compositions |
| CN104350372A (en) * | 2012-08-09 | 2015-02-11 | 斯坦福大学托管董事会 | Methods and compositions for preparing biological specimens for microscopic analysis |
| CN204758528U (en) * | 2015-06-15 | 2015-11-11 | 南昌大学 | Transparent device of biological tissue of integration |
-
2015
- 2015-06-15 CN CN201510328214.6A patent/CN104965016A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004011144A2 (en) * | 2002-07-29 | 2004-02-05 | Dumas David P | Transparent polymer support for electrophoresis and electrochromatography and related methods |
| US20120064136A1 (en) * | 2010-09-10 | 2012-03-15 | Nanobio Corporation | Anti-aging and wrinkle treatment methods using nanoemulsion compositions |
| CN104350372A (en) * | 2012-08-09 | 2015-02-11 | 斯坦福大学托管董事会 | Methods and compositions for preparing biological specimens for microscopic analysis |
| CN204758528U (en) * | 2015-06-15 | 2015-11-11 | 南昌大学 | Transparent device of biological tissue of integration |
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