HRP20230991T1 - Inzulin-fc fuzijski proteini ultradugog djelovanja i metode uporabe - Google Patents
Inzulin-fc fuzijski proteini ultradugog djelovanja i metode uporabe Download PDFInfo
- Publication number
- HRP20230991T1 HRP20230991T1 HRP20230991TT HRP20230991T HRP20230991T1 HR P20230991 T1 HRP20230991 T1 HR P20230991T1 HR P20230991T T HRP20230991T T HR P20230991TT HR P20230991 T HRP20230991 T HR P20230991T HR P20230991 T1 HRP20230991 T1 HR P20230991T1
- Authority
- HR
- Croatia
- Prior art keywords
- fusion protein
- cell culture
- nucleic acid
- image
- cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 9
- 102000037865 fusion proteins Human genes 0.000 title claims 8
- 108020001507 fusion proteins Proteins 0.000 title claims 8
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 102000018071 Immunoglobulin Fc Fragments Human genes 0.000 claims 3
- 108010091135 Immunoglobulin Fc Fragments Proteins 0.000 claims 3
- 102000004877 Insulin Human genes 0.000 claims 3
- 108090001061 Insulin Proteins 0.000 claims 3
- 239000006143 cell culture medium Substances 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 238000004113 cell culture Methods 0.000 claims 2
- 239000002299 complementary DNA Substances 0.000 claims 2
- 239000012228 culture supernatant Substances 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- 238000001890 transfection Methods 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 238000003153 stable transfection Methods 0.000 claims 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/65—Peptidic linkers, binders or spacers, e.g. peptidic enzyme-labile linkers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
- A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
- A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
- A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2869—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against hormone receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Diabetes (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Dermatology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Emergency Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Neurology (AREA)
- Analytical Chemistry (AREA)
Claims (7)
1. Metoda pripreme rekombinantne stanice koja sadrži nukleinsku kiselinu koja kodira fuzijski protein, navedeni fuzijski protein sadrži polipeptid inzulina i Fc fragment,
pri čemu su polipeptid inzulina i Fc fragment povezani poveznicom, pri čemu Fc fragment sadrži sljedeću sekvencu:
[image]
i pri čemu polipeptid inzulina sadrži sljedeću sekvencu:
[image]
i pri čemu Xi nije D i X2 nije H,
navedena metoda sadrži:
transfektiranje stanice domaćina s nukleinskom kiselinom koja kodira navedeni fuzijski protein, pri čemu se navedeni fuzijski protein eksprimira u navedenoj rekombinantnoj stanici nakon navedenog koraka transfekcije.
2. Metoda prema zahtjevu 1, nadalje sadrži:
uzgoj navedene rekombinantne stanice u mediju stanične kulture; i
prikupljanje supernatanta stanične kulture iz navedenog medija stanične kulture, navedeni supernatant stanične kulture sadrži navedeni eksprimirani fuzijski protein.
3. Metoda prema zahtjevu 2, nadalje sadrži:
pročišćavanje ili izolaciju iz medija kulture stanica.
4. Metoda prema zahtjevu 3, naznačena time što navedeni koraci pročišćavanja ili izolacije sadrže centrifugiranje, filtraciju, i/ili kromatografiju.
5. Metoda prema zahtjevu 1, naznačena time što navedeni korak transfekcije sadrži stabilnu transfekciju navedene stanice domaćina.
6. Metoda prema zahtjevu 1, naznačena time što navedena nukleinska kiselina sadrži cDNA koja kodira navedeni fuzijski protein, navedeni fuzijski protein sadrži sljedeću sekvencu:
[image]
7. Metoda prema zahtjevu 6, naznačena time što cDNA sadrži sljedeću sekvencu nukleinske kiseline:
[image]
Applications Claiming Priority (16)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862692498P | 2018-06-29 | 2018-06-29 | |
US201862692507P | 2018-06-29 | 2018-06-29 | |
US201862693814P | 2018-07-03 | 2018-07-03 | |
US201862696645P | 2018-07-11 | 2018-07-11 | |
US201862698648P | 2018-07-16 | 2018-07-16 | |
US201862702167P | 2018-07-23 | 2018-07-23 | |
US201862719347P | 2018-08-17 | 2018-08-17 | |
US201862740735P | 2018-10-03 | 2018-10-03 | |
US201862743358P | 2018-10-09 | 2018-10-09 | |
US201862774682P | 2018-12-03 | 2018-12-03 | |
US201862781378P | 2018-12-18 | 2018-12-18 | |
US201862781368P | 2018-12-18 | 2018-12-18 | |
US201962824176P | 2019-03-26 | 2019-03-26 | |
US201962827809P | 2019-04-01 | 2019-04-01 | |
US201962837188P | 2019-04-22 | 2019-04-22 | |
EP21207279.7A EP4011908B1 (en) | 2018-06-29 | 2019-06-28 | Ultra-long acting insulin-fc fusion proteins and methods of use |
Publications (1)
Publication Number | Publication Date |
---|---|
HRP20230991T1 true HRP20230991T1 (hr) | 2023-12-08 |
Family
ID=68985236
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HRP20220091TT HRP20220091T1 (hr) | 2018-06-29 | 2019-06-28 | Inzulin-fc fuzijski proteini ultradugog djelovanja i postupci uporabe |
HRP20240408TT HRP20240408T1 (hr) | 2018-06-29 | 2019-06-28 | Inzulin-fc fuzijski proteini ultradugog djelovanja i metode uporabe |
HRP20221418TT HRP20221418T1 (hr) | 2018-06-29 | 2019-06-28 | Inzulin-fc fuzijski proteini ultra-dugog djelovanja i postupci uporabe |
HRP20230991TT HRP20230991T1 (hr) | 2018-06-29 | 2019-06-28 | Inzulin-fc fuzijski proteini ultradugog djelovanja i metode uporabe |
Family Applications Before (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HRP20220091TT HRP20220091T1 (hr) | 2018-06-29 | 2019-06-28 | Inzulin-fc fuzijski proteini ultradugog djelovanja i postupci uporabe |
HRP20240408TT HRP20240408T1 (hr) | 2018-06-29 | 2019-06-28 | Inzulin-fc fuzijski proteini ultradugog djelovanja i metode uporabe |
HRP20221418TT HRP20221418T1 (hr) | 2018-06-29 | 2019-06-28 | Inzulin-fc fuzijski proteini ultra-dugog djelovanja i postupci uporabe |
Country Status (15)
Country | Link |
---|---|
US (8) | US10947292B2 (hr) |
EP (5) | EP4011908B1 (hr) |
JP (2) | JP7417277B2 (hr) |
KR (1) | KR20210029210A (hr) |
CN (1) | CN113038964A (hr) |
AU (1) | AU2019291945A1 (hr) |
BR (1) | BR112020026777A2 (hr) |
CA (1) | CA3104144A1 (hr) |
DK (4) | DK3655006T3 (hr) |
FI (2) | FI4186920T3 (hr) |
HR (4) | HRP20220091T1 (hr) |
HU (4) | HUE065725T2 (hr) |
LT (4) | LT3892628T (hr) |
SI (4) | SI3892628T1 (hr) |
WO (1) | WO2020006529A1 (hr) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK3551209T3 (da) | 2016-12-09 | 2021-08-23 | Akston Biosciences Corp | Insulin-fc-fusioner og fremgangsmåder til anvendelse |
US11267862B2 (en) | 2018-06-29 | 2022-03-08 | Akston Biosciences Corporation | Ultra-long acting insulin-Fc fusion proteins and methods of use |
LT3892628T (lt) | 2018-06-29 | 2022-12-12 | Akston Biosciences Corporation | Itin ilgai veikiantys insulino-fc sulieti baltymai ir naudojimo būdai |
WO2021011827A1 (en) * | 2019-07-16 | 2021-01-21 | Akston Biosciences Corporation | Ultra-long acting insulin-fc fusion proteins and methods of use |
US11352407B2 (en) | 2019-12-19 | 2022-06-07 | Akston Biosciences Corporation | Ultra-long acting insulin-Fc fusion proteins |
US11186623B2 (en) | 2019-12-24 | 2021-11-30 | Akston Bioscience Corporation | Ultra-long acting insulin-Fc fusion proteins and methods of use |
EP4248983A3 (en) | 2020-04-10 | 2024-01-24 | Akston Biosciences Corporation | Antigen specific immunotherapy for covid-19 fusion proteins and methods of use |
US11192930B2 (en) | 2020-04-10 | 2021-12-07 | Askton Bioscences Corporation | Ultra-long acting insulin-Fc fusion protein and methods of use |
US11198719B2 (en) | 2020-04-29 | 2021-12-14 | Akston Biosciences Corporation | Ultra-long acting insulin-Fc fusion protein and methods of use |
EP4230216A1 (en) * | 2020-07-24 | 2023-08-23 | Jiangsu Gensciences Inc. | Insulin-fc fusion protein and application thereof |
TW202237175A (zh) * | 2020-12-14 | 2022-10-01 | 美商美國禮來大藥廠 | 治療糖尿病之方法 |
WO2023004406A2 (en) | 2021-07-23 | 2023-01-26 | Akston Biosciences Corporation | Insulin-fc fusion proteins and methods of use to treat cancer |
WO2023064711A2 (en) * | 2021-10-14 | 2023-04-20 | Akston Biosciences Corporation | Ultra-long acting insulin-fc fusion proteins and methods of use |
AR127619A1 (es) | 2021-11-15 | 2024-02-14 | Lilly Co Eli | FORMULACIONES CONSERVADAS DE FUSIONES DE INSULINA-Fc |
TW202342552A (zh) * | 2022-03-16 | 2023-11-01 | 大陸商北京拓界生物醫藥科技有限公司 | 人胰島素類似物、其融合蛋白及醫藥用途 |
WO2023247640A1 (en) * | 2022-06-23 | 2023-12-28 | Sanofi | Single chain insulins and fc conjugates thereof |
Family Cites Families (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030040601A1 (en) | 2001-06-08 | 2003-02-27 | Ivan Diers | Method for making insulin precursors and insulin analog precursors |
US7169894B2 (en) * | 2001-11-14 | 2007-01-30 | Mirari Biosciences, Inc. | Methods and compositions for reverse translation |
US8188231B2 (en) | 2002-09-27 | 2012-05-29 | Xencor, Inc. | Optimized FC variants |
US20100143479A1 (en) | 2008-12-04 | 2010-06-10 | Oakwood Laboratories, Llc | Method of making sustained release microparticles |
WO2010107520A1 (en) | 2009-03-20 | 2010-09-23 | Smartcells, Inc. | Soluble non-depot insulin conjugates and uses thereof |
WO2010117760A2 (en) * | 2009-03-30 | 2010-10-14 | Boehringer Ingelheim International Gmbh | Fusion proteins comprising canine fc portions |
CN101891823B (zh) | 2010-06-11 | 2012-10-03 | 北京东方百泰生物科技有限公司 | 一种Exendin-4及其类似物融合蛋白 |
EP2598170A4 (en) | 2010-07-28 | 2016-07-06 | Smartcells Inc | MEDICAMENT-LIGAND CONJUGATES, THEIR SYNTHESIS AND CORRESPONDING INTERMEDIATES |
AU2011282988A1 (en) | 2010-07-28 | 2013-01-31 | Smartcells, Inc. | Recombinantly expressed insulin polypeptides and uses thereof |
AR087433A1 (es) | 2011-08-08 | 2014-03-26 | Merck Sharp & Dohme | Analogos de insulina n-glicosilados |
WO2013074598A1 (en) * | 2011-11-18 | 2013-05-23 | Merck Sharp & Dohme Corp. | Fc CONTAINING POLYPEPTIDES HAVING INCREASED ANTI-INFLAMMATORY PROPERTIES AND INCREASED FcRN BINDING |
KR102041412B1 (ko) | 2011-12-30 | 2019-11-11 | 한미사이언스 주식회사 | 면역글로불린 Fc 단편 유도체 |
CN103509118B (zh) * | 2012-06-15 | 2016-03-23 | 郭怀祖 | 胰岛素-Fc融合蛋白 |
AU2013302569B2 (en) * | 2012-08-17 | 2017-09-28 | Dresser-Rand Company | System and method for detecting stall or surge in radial compressors |
WO2014093387A1 (en) * | 2012-12-10 | 2014-06-19 | Kindred Biosciences, Inc. | Vegf receptor fusion proteins for veterinary use |
PE20151409A1 (es) | 2013-02-26 | 2015-10-07 | Hanmi Pharm Ind Co Ltd | Analogo de insulina novedoso y su uso |
WO2016044676A2 (en) * | 2014-09-18 | 2016-03-24 | AskGene Pharma, Inc. | Novel feline erythropoietin receptor agonists |
CA2961037A1 (en) | 2014-10-06 | 2016-04-14 | Case Western Reserve University | Biphasic single-chain insulin analogues |
WO2016064606A1 (en) | 2014-10-20 | 2016-04-28 | Case Western Reserve University | Halogenated insulin analogues of enhanced biological potency |
WO2016105545A2 (en) | 2014-12-24 | 2016-06-30 | Case Western Reserve University | Insulin analogues with enhanced stabilized and reduced mitogenicity |
CN107428814A (zh) | 2015-01-20 | 2017-12-01 | 卡斯西部储备大学 | 具有选择性信号传导特性和降低的促有丝***性的胰岛素类似物 |
JP2018504911A (ja) * | 2015-01-29 | 2018-02-22 | ネックスヴェット オーストラリア プロプライエタリー リミテッド | 治療及び診断剤 |
US10894089B2 (en) | 2015-02-17 | 2021-01-19 | Hanmi Pharm. Co., Ltd. | Long-acting insulin or insulin analogue conjugate |
ES2754432T3 (es) * | 2015-05-04 | 2020-04-17 | Apogenix Ag | Proteínas agonistas de receptor CD40 de cadena sencilla |
AR105616A1 (es) * | 2015-05-07 | 2017-10-25 | Lilly Co Eli | Proteínas de fusión |
ES2784603T3 (es) | 2015-06-02 | 2020-09-29 | Novo Nordisk As | Insulinas con extensiones recombinantes polares |
EP3757119A1 (en) | 2015-11-16 | 2020-12-30 | Ubiprotein, Corp. | A method for extending half-life of a protein |
WO2018014091A1 (en) | 2016-07-22 | 2018-01-25 | University Of Utah Research Foundation | Insulin analogs |
CN109071623B (zh) * | 2016-05-04 | 2022-05-27 | 美国安进公司 | 用于扩增t调节性细胞的白细胞介素-2突变蛋白 |
WO2018009921A1 (en) * | 2016-07-08 | 2018-01-11 | AskGene Pharma, Inc. | Fusion protein comprising leptin and methods for producing and using the same |
TWI774694B (zh) * | 2016-09-23 | 2022-08-21 | 南韓商韓美藥品股份有限公司 | 具有降低對胰島素受體之親和力之胰島素類似物及其用途 |
WO2018073185A1 (en) | 2016-10-17 | 2018-04-26 | Vetoquinol Sa | Modified antibody constant region |
DK3551209T3 (da) | 2016-12-09 | 2021-08-23 | Akston Biosciences Corp | Insulin-fc-fusioner og fremgangsmåder til anvendelse |
TW201835099A (zh) | 2017-03-07 | 2018-10-01 | 美國凱斯西方瑞瑟夫大學 | 藉由第四雙硫鍵穩定之單鏈胰島素類似物 |
US10572594B2 (en) | 2017-08-01 | 2020-02-25 | Intuit Inc. | Extracting domain-specific actions and entities in natural language commands recognized based on edition and recognition scores |
MX2020001707A (es) | 2017-08-15 | 2020-03-20 | Kindred Biosciences Inc | Variantes igg fc para uso veterinario. |
CN112423741A (zh) | 2018-04-16 | 2021-02-26 | 犹他大学研究基金会 | 葡萄糖反应性胰岛素 |
LT3892628T (lt) | 2018-06-29 | 2022-12-12 | Akston Biosciences Corporation | Itin ilgai veikiantys insulino-fc sulieti baltymai ir naudojimo būdai |
US11267862B2 (en) | 2018-06-29 | 2022-03-08 | Akston Biosciences Corporation | Ultra-long acting insulin-Fc fusion proteins and methods of use |
WO2020069011A1 (en) | 2018-09-25 | 2020-04-02 | Absci, Llc | Protein purification methods |
US11649269B2 (en) | 2018-10-05 | 2023-05-16 | Novo Nordisk A/S | Bifunctional compounds comprising insulin peptides and EGF(A) peptides |
CN113330025A (zh) | 2018-11-19 | 2021-08-31 | 卡斯西部储备大学 | 具有聚丙氨酸c结构域子区段的单链胰岛素类似物 |
AU2020279968A1 (en) | 2019-05-17 | 2021-12-16 | Case Western Reserve University | Variant single-chain insulin analogues |
WO2021011827A1 (en) | 2019-07-16 | 2021-01-21 | Akston Biosciences Corporation | Ultra-long acting insulin-fc fusion proteins and methods of use |
JP2022542301A (ja) | 2019-07-31 | 2022-09-30 | イーライ リリー アンド カンパニー | リラキシン類似体およびその使用方法 |
US11352407B2 (en) | 2019-12-19 | 2022-06-07 | Akston Biosciences Corporation | Ultra-long acting insulin-Fc fusion proteins |
US11186623B2 (en) | 2019-12-24 | 2021-11-30 | Akston Bioscience Corporation | Ultra-long acting insulin-Fc fusion proteins and methods of use |
US11192930B2 (en) | 2020-04-10 | 2021-12-07 | Askton Bioscences Corporation | Ultra-long acting insulin-Fc fusion protein and methods of use |
US11198719B2 (en) | 2020-04-29 | 2021-12-14 | Akston Biosciences Corporation | Ultra-long acting insulin-Fc fusion protein and methods of use |
-
2019
- 2019-06-28 LT LTEP21164434.9T patent/LT3892628T/lt unknown
- 2019-06-28 HU HUE22203969A patent/HUE065725T2/hu unknown
- 2019-06-28 HR HRP20220091TT patent/HRP20220091T1/hr unknown
- 2019-06-28 HR HRP20240408TT patent/HRP20240408T1/hr unknown
- 2019-06-28 LT LTEPPCT/US2019/040010T patent/LT3655006T/lt unknown
- 2019-06-28 CA CA3104144A patent/CA3104144A1/en active Pending
- 2019-06-28 HU HUE21164434A patent/HUE060471T2/hu unknown
- 2019-06-28 SI SI201930392T patent/SI3892628T1/sl unknown
- 2019-06-28 AU AU2019291945A patent/AU2019291945A1/en active Pending
- 2019-06-28 BR BR112020026777-5A patent/BR112020026777A2/pt unknown
- 2019-06-28 HR HRP20221418TT patent/HRP20221418T1/hr unknown
- 2019-06-28 EP EP21207279.7A patent/EP4011908B1/en active Active
- 2019-06-28 DK DK19826154.7T patent/DK3655006T3/da active
- 2019-06-28 SI SI201930726T patent/SI4186920T1/sl unknown
- 2019-06-28 DK DK22203969.5T patent/DK4186920T5/da active
- 2019-06-28 JP JP2020573352A patent/JP7417277B2/ja active Active
- 2019-06-28 DK DK21207279.7T patent/DK4011908T3/da active
- 2019-06-28 DK DK21164434.9T patent/DK3892628T3/da active
- 2019-06-28 HU HUE21207279A patent/HUE062719T2/hu unknown
- 2019-06-28 HR HRP20230991TT patent/HRP20230991T1/hr unknown
- 2019-06-28 EP EP19826154.7A patent/EP3655006B1/en active Active
- 2019-06-28 EP EP21164434.9A patent/EP3892628B1/en active Active
- 2019-06-28 WO PCT/US2019/040010 patent/WO2020006529A1/en active Search and Examination
- 2019-06-28 LT LTEP22203969.5T patent/LT4186920T/lt unknown
- 2019-06-28 KR KR1020217002135A patent/KR20210029210A/ko unknown
- 2019-06-28 EP EP22203969.5A patent/EP4186920B1/en active Active
- 2019-06-28 FI FIEP22203969.5T patent/FI4186920T3/fi active
- 2019-06-28 SI SI201930615T patent/SI4011908T1/sl unknown
- 2019-06-28 LT LTEP21207279.7T patent/LT4011908T/lt unknown
- 2019-06-28 HU HUE19826154A patent/HUE058005T2/hu unknown
- 2019-06-28 FI FIEP21207279.7T patent/FI4011908T3/fi active
- 2019-06-28 CN CN201980056947.5A patent/CN113038964A/zh active Pending
- 2019-06-28 EP EP23215091.2A patent/EP4344738A3/en active Pending
- 2019-06-28 SI SI201930152T patent/SI3655006T1/sl unknown
-
2020
- 2020-01-29 US US16/776,136 patent/US10947292B2/en active Active
- 2020-01-29 US US16/776,065 patent/US10870686B2/en active Active
- 2020-01-29 US US16/775,979 patent/US10961294B2/en active Active
- 2020-01-29 US US16/776,180 patent/US10851147B2/en active Active
- 2020-09-11 US US17/019,059 patent/US11261229B2/en active Active
-
2021
- 2021-09-29 US US17/488,852 patent/US11773151B2/en active Active
- 2021-09-29 US US17/488,685 patent/US11673934B2/en active Active
-
2023
- 2023-09-01 US US18/459,581 patent/US20240101635A1/en active Pending
- 2023-12-25 JP JP2023217822A patent/JP7510728B2/ja active Active
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
HRP20230991T1 (hr) | Inzulin-fc fuzijski proteini ultradugog djelovanja i metode uporabe | |
US5989868A (en) | Fusion protein systems designed to increase soluble cytoplasmic expression of heterologous proteins in esherichia coli | |
RU2019127861A (ru) | ВАРИАНТЫ Fc С ИЗМЕНЕННЫМ СВЯЗЫВАНИЕМ С FcRn | |
WO2020139783A3 (en) | Polypeptides useful for gene editing and methods of use | |
WO2006069403B1 (en) | Methods for producing soluble multi-membrane-spanning proteins | |
AR051837A1 (es) | Produccion de proteinas de fusion recombinante dentro de ensamblajes similares a cuerpos proteicos recombinantes (escpr) | |
JP2012531208A5 (hr) | ||
JP2016520053A5 (hr) | ||
US10227401B2 (en) | Production cell line enhancers | |
JP2020509755A5 (hr) | ||
PH12017502080A1 (en) | Epidermal growth factor receptor variant iii-mesothelin fusions and methods of using the same | |
CN103131676A (zh) | 表达重组人肿瘤坏死因子受体-Fc融合蛋白基因的家蚕重组杆状病毒及其制备方法和应用 | |
CN106478785A (zh) | 一种鸡贫血病毒凋亡素融合重组蛋白及其制备方法和应用 | |
US20120196328A1 (en) | Fusion protein mixture for inducing human pluripotent stem cell and preparation method there of | |
CN102071214A (zh) | 人胱抑素c真核表达载体的构建及其表达、纯化 | |
CN103205444A (zh) | 一种人活性颗粒酶k重组蛋白的制备方法 | |
CN102276727B (zh) | IL-17RC-hFc融合蛋白及其应用 | |
CN107936111B (zh) | 一种hip/pap蛋白的制备方法 | |
CN105601726B (zh) | Oct4在调控IL-31基因表达中的应用 | |
CN107083369B (zh) | 抗树鼩cd3分子单克隆抗体及分泌该抗体的杂交瘤细胞株与应用 | |
CN104892758A (zh) | 利用gst表达体系制备aqp7胞外段抗体及应用 | |
CN103555742A (zh) | 一种人活性颗粒酶a重组蛋白的制备方法 | |
CN100363497C (zh) | 基因重组技术制备pgrp(31-98)片段的方法 | |
KR102121817B1 (ko) | Crispr 편집 기술을 이용한 재조합 항원을 발현시키는 벡터 및 이를 동시에 다중 삽입시키는 방법 | |
WO2016005903A4 (en) | A process for obtaining exendin-4 |