HRP20220521T1 - Ciljana genska modifikacija primjenom popravka gena posredovanog oligonukleotidom - Google Patents
Ciljana genska modifikacija primjenom popravka gena posredovanog oligonukleotidom Download PDFInfo
- Publication number
- HRP20220521T1 HRP20220521T1 HRP20220521TT HRP20220521T HRP20220521T1 HR P20220521 T1 HRP20220521 T1 HR P20220521T1 HR P20220521T T HRP20220521T T HR P20220521TT HR P20220521 T HRP20220521 T HR P20220521T HR P20220521 T1 HRP20220521 T1 HR P20220521T1
- Authority
- HR
- Croatia
- Prior art keywords
- nickases
- gron
- site
- cell
- dna sequence
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims 4
- 230000008439 repair process Effects 0.000 title claims 3
- 108091034117 Oligonucleotide Proteins 0.000 title claims 2
- 230000001404 mediated effect Effects 0.000 title claims 2
- 238000012239 gene modification Methods 0.000 title 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 18
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 18
- 238000000034 method Methods 0.000 claims 16
- 210000004027 cell Anatomy 0.000 claims 11
- 241000196324 Embryophyta Species 0.000 claims 7
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 238000006243 chemical reaction Methods 0.000 claims 6
- 108020004414 DNA Proteins 0.000 claims 3
- 102000053602 DNA Human genes 0.000 claims 3
- 240000003829 Sorghum propinquum Species 0.000 claims 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims 3
- 238000010459 TALEN Methods 0.000 claims 3
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 3
- 108091005948 blue fluorescent proteins Proteins 0.000 claims 3
- 210000001938 protoplast Anatomy 0.000 claims 3
- 239000011701 zinc Substances 0.000 claims 3
- 229910052725 zinc Inorganic materials 0.000 claims 3
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 claims 2
- 235000011293 Brassica napus Nutrition 0.000 claims 2
- 240000002791 Brassica napus Species 0.000 claims 2
- 235000006008 Brassica napus var napus Nutrition 0.000 claims 2
- 240000000385 Brassica napus var. napus Species 0.000 claims 2
- 240000007124 Brassica oleracea Species 0.000 claims 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims 2
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 claims 2
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 claims 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims 2
- 230000033616 DNA repair Effects 0.000 claims 2
- 235000010469 Glycine max Nutrition 0.000 claims 2
- 244000068988 Glycine max Species 0.000 claims 2
- 240000005979 Hordeum vulgare Species 0.000 claims 2
- 235000007340 Hordeum vulgare Nutrition 0.000 claims 2
- 240000004658 Medicago sativa Species 0.000 claims 2
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims 2
- 235000010582 Pisum sativum Nutrition 0.000 claims 2
- 240000004713 Pisum sativum Species 0.000 claims 2
- 240000008042 Zea mays Species 0.000 claims 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 2
- 235000005822 corn Nutrition 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- 244000291564 Allium cepa Species 0.000 claims 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 claims 1
- 244000075850 Avena orientalis Species 0.000 claims 1
- 235000007319 Avena orientalis Nutrition 0.000 claims 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 claims 1
- 241000219198 Brassica Species 0.000 claims 1
- 235000003351 Brassica cretica Nutrition 0.000 claims 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 claims 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims 1
- 235000004221 Brassica oleracea var gemmifera Nutrition 0.000 claims 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims 1
- 235000012905 Brassica oleracea var viridis Nutrition 0.000 claims 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 claims 1
- 244000308368 Brassica oleracea var. gemmifera Species 0.000 claims 1
- 235000003343 Brassica rupestris Nutrition 0.000 claims 1
- 235000004936 Bromus mango Nutrition 0.000 claims 1
- 235000005881 Calendula officinalis Nutrition 0.000 claims 1
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 235000002566 Capsicum Nutrition 0.000 claims 1
- 235000005633 Chrysanthemum balsamita Nutrition 0.000 claims 1
- 244000260524 Chrysanthemum balsamita Species 0.000 claims 1
- 235000010523 Cicer arietinum Nutrition 0.000 claims 1
- 244000045195 Cicer arietinum Species 0.000 claims 1
- 241000207199 Citrus Species 0.000 claims 1
- 229920000742 Cotton Polymers 0.000 claims 1
- 241000219112 Cucumis Species 0.000 claims 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims 1
- 240000008067 Cucumis sativus Species 0.000 claims 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims 1
- 235000002767 Daucus carota Nutrition 0.000 claims 1
- 244000000626 Daucus carota Species 0.000 claims 1
- 235000009355 Dianthus caryophyllus Nutrition 0.000 claims 1
- 240000006497 Dianthus caryophyllus Species 0.000 claims 1
- 244000004281 Eucalyptus maculata Species 0.000 claims 1
- 240000009088 Fragaria x ananassa Species 0.000 claims 1
- 241000219146 Gossypium Species 0.000 claims 1
- 244000020551 Helianthus annuus Species 0.000 claims 1
- 235000003222 Helianthus annuus Nutrition 0.000 claims 1
- 235000003228 Lactuca sativa Nutrition 0.000 claims 1
- 240000008415 Lactuca sativa Species 0.000 claims 1
- 240000004322 Lens culinaris Species 0.000 claims 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 claims 1
- 241000234435 Lilium Species 0.000 claims 1
- 235000004431 Linum usitatissimum Nutrition 0.000 claims 1
- 240000006240 Linum usitatissimum Species 0.000 claims 1
- 241000219745 Lupinus Species 0.000 claims 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims 1
- 244000070406 Malus silvestris Species 0.000 claims 1
- 235000014826 Mangifera indica Nutrition 0.000 claims 1
- 240000007228 Mangifera indica Species 0.000 claims 1
- 240000005561 Musa balbisiana Species 0.000 claims 1
- 240000002853 Nelumbo nucifera Species 0.000 claims 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims 1
- 244000061176 Nicotiana tabacum Species 0.000 claims 1
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 239000006002 Pepper Substances 0.000 claims 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims 1
- 241000018646 Pinus brutia Species 0.000 claims 1
- 235000011613 Pinus brutia Nutrition 0.000 claims 1
- 235000016761 Piper aduncum Nutrition 0.000 claims 1
- 240000003889 Piper guineense Species 0.000 claims 1
- 235000017804 Piper guineense Nutrition 0.000 claims 1
- 235000008184 Piper nigrum Nutrition 0.000 claims 1
- 241000219000 Populus Species 0.000 claims 1
- 240000005809 Prunus persica Species 0.000 claims 1
- 235000006040 Prunus persica var persica Nutrition 0.000 claims 1
- 241000220324 Pyrus Species 0.000 claims 1
- 240000000111 Saccharum officinarum Species 0.000 claims 1
- 235000007201 Saccharum officinarum Nutrition 0.000 claims 1
- 241000209056 Secale Species 0.000 claims 1
- 235000007238 Secale cereale Nutrition 0.000 claims 1
- 240000003768 Solanum lycopersicum Species 0.000 claims 1
- 235000002597 Solanum melongena Nutrition 0.000 claims 1
- 244000061458 Solanum melongena Species 0.000 claims 1
- 235000002595 Solanum tuberosum Nutrition 0.000 claims 1
- 244000061456 Solanum tuberosum Species 0.000 claims 1
- 235000009184 Spondias indica Nutrition 0.000 claims 1
- 235000021536 Sugar beet Nutrition 0.000 claims 1
- 240000000785 Tagetes erecta Species 0.000 claims 1
- 235000021307 Triticum Nutrition 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 241000722921 Tulipa gesneriana Species 0.000 claims 1
- 235000010749 Vicia faba Nutrition 0.000 claims 1
- 240000006677 Vicia faba Species 0.000 claims 1
- 235000002096 Vicia faba var. equina Nutrition 0.000 claims 1
- 235000002098 Vicia faba var. major Nutrition 0.000 claims 1
- 241000219094 Vitaceae Species 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 235000021016 apples Nutrition 0.000 claims 1
- 235000021015 bananas Nutrition 0.000 claims 1
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 claims 1
- 108091092356 cellular DNA Proteins 0.000 claims 1
- 239000013611 chromosomal DNA Substances 0.000 claims 1
- 235000020971 citrus fruits Nutrition 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 235000005489 dwarf bean Nutrition 0.000 claims 1
- 238000011156 evaluation Methods 0.000 claims 1
- 235000021021 grapes Nutrition 0.000 claims 1
- 244000080020 horsebean Species 0.000 claims 1
- 235000010460 mustard Nutrition 0.000 claims 1
- 230000000869 mutational effect Effects 0.000 claims 1
- 235000021017 pears Nutrition 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 235000012015 potatoes Nutrition 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 235000021012 strawberries Nutrition 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Physiology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Claims (14)
1. Postupak za uvođenje mutacije posredovane oligonukleobazom za popravak gena (GRON) u ciljnu sekvencu deoksiribonukleinske kiseline (DNK) u biljnoj stanici, koji obuhvaća:
kultivaciju stanice pod uvjetima koji povećavaju jedan ili više procesa popravke DNK stanice prije, i/ili istovremeno sa, isporukom GRON-a u biljnu stanicu,
pri čemu uvjeti koji povećavaju jedan ili više procesa popravka stanične DNK obuhvaćaju uvođenje jedne ili više nikaza specifičnih za mjesto u biljnu stanicu,
pri čemu jedna ili više nikaza specifičnih za mjesto uvode ureze jednog niza u blizini ciljanog mjesta za konverziju pomoću GRON-a.
2. Postupak prema patentnom zahtjevu 1, pri čemu jedna ili više nikaza su odabrane od TALEN nikaza, nikaza cinkovog prsta i meganukleaznih nikaza.
3. Postupak prema patentnom zahtjevu 1 ili 2, pri čemu jedna ili više nikaza specifičnih za mjesto su kovalentno povezane za GRON.
4. Postupak prema jednom od patentnih zahtjeva 1-3, pri čemu stanica je biljna stanica vrste koja je odabrana iz grupe koja se sastoji od repice, suncokreta, kukuruza, duhana, šećerne repe, pamuka, kukuruza, pšenice, ječma, riže, lucerne, ječma, sirka, rajčice, manga, breskve, jabuke, kruške, jagode, banane, dinje, krumpira, mrkve, zelene salate, luka, zrna soje, više vrsta soje, šećerne trske, graška, slanutka, poljskog graška, boba, leće, repe, korabe, prokulice, lupine, cvjetače, kelja, poljskog graha, topole, bora, eukaliptusa, grožđa, citrusa, pšenoraži, lucerne, raži, zobi, travnjaka i krmne trave, lana, uljane repice, gorušice, krastavca, slaka, nedirka, papra, patlidžana, nevena, lotosa, kupusa, tratinčice, karanfila, tulipana, irisa i ljiljana.
5. Postupak prema jednom od patentnih zahtjeva 1-4, pri čemu je stanica transgena.
6. Postupak prema patentnom zahtjevu 5, pri čemu ciljna DNK sekvenca je endogeni gen stanice.
7. Postupak prema jednom od patentnih zahtjeva 1-6, pri čemu postupak dalje obuhvaća regeneriranje biljke koja ima mutaciju uvedenu pomoću GRON-a iz biljne stanice.
8. Primjena jedne ili više nikaza za poboljšanje mutacijske konverzije pomoću oligonukleotida za popravak gena (GRON-ova) kao donora, koja obuhvaća uvođenje jedne ili više nikaza specifičnih za mjesto u stanicu.
9. Primjena prema patentnom zahtjevu 8, pri čemu su jedna ili više nikaza odabrane od TALEN nikaza, nikaza cinkovog prsta i meganukleaznih nikaza.
10. Postupak prema patentnom zahtjevu 1, koji dalje obuhvaća procjenu efikasnosti konverzija za GRON, pri čemu postupak obuhvata:
uvođenje GRON-a u biljni protoplast koji eksprimira ciljnu DNK sekvencu koja kodira plavi fluorescentni protein, pri čemu je GRON konfiguriran da mutira ciljnu DNK sekvencu na unaprijed određenom mjestu da izazove konverziju plavog fluorescentnog proteina pri čemu plavi fluorescentni protein emitira fluorescenciju na drugačijoj valnoj dužini, pri čemu prije ili istovremeno sa uvođenjem GRON-a, DNK u protoplastu dolazi u kontakt sa navedenom jednom ili više nikaza specifičnih za mjesto koje uvode ureze jednog niza u ciljnu DNK sekvencu unutar mjesta ciljanog pomoću GRON-a; i
određivanje efikasnosti konverzije.
11. Postupak prema patentnom zahtjevu 10, pri čemu jedna ili više nikaza specifičnih za mjesto su odabrane od TALEN nikaza, nikaza cink prsta i meganukleaznih nikaza.
12. Postupak prema patentnom zahtjevu 10 ili 11, pri čemu ciljna DNK sekvenca je prisutna u kromosomskoj DNK protoplasta
13. Postupak prema patentnom zahtjevu 10 ili 11, pri čemu ciljna DNK sekvenca je prisutna u plazmidu.
14. Postupak prema jednom od patentnih zahtjeva 10-13, pri čemu jedna ili više nikaza specifičnih za mjesto osiguravaju povećanu efikasnost konverzije u odnosu na efikasnost određenu za GRON u odsustvu jedne ili više nikaza.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361801320P | 2013-03-15 | 2013-03-15 | |
| EP14763072.7A EP2966984B1 (en) | 2013-03-15 | 2014-03-14 | Targeted gene modification using oligonucleotide-mediated gene repair |
| PCT/US2014/029621 WO2014144987A2 (en) | 2013-03-15 | 2014-03-14 | Methods and compositions for increasing efficiency of increased efficiency of targeted gene modification using oligonucleotide-mediated gene repair |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20220521T1 true HRP20220521T1 (hr) | 2022-06-10 |
Family
ID=51538403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20220521TT HRP20220521T1 (hr) | 2013-03-15 | 2014-03-14 | Ciljana genska modifikacija primjenom popravka gena posredovanog oligonukleotidom |
Country Status (24)
| Country | Link |
|---|---|
| US (2) | US11434494B2 (hr) |
| EP (1) | EP2966984B1 (hr) |
| JP (1) | JP2016516408A (hr) |
| KR (1) | KR102304487B1 (hr) |
| CN (1) | CN105338805A (hr) |
| AU (2) | AU2014233465A1 (hr) |
| BR (1) | BR112015022848B1 (hr) |
| CA (1) | CA2905135C (hr) |
| CL (1) | CL2015002650A1 (hr) |
| DK (1) | DK2966984T3 (hr) |
| EA (1) | EA201591447A1 (hr) |
| ES (1) | ES2912400T3 (hr) |
| HK (1) | HK1220864A1 (hr) |
| HR (1) | HRP20220521T1 (hr) |
| HU (1) | HUE058351T2 (hr) |
| IL (1) | IL241145B (hr) |
| LT (1) | LT2966984T (hr) |
| MY (1) | MY191390A (hr) |
| PL (1) | PL2966984T3 (hr) |
| PT (1) | PT2966984T (hr) |
| RS (1) | RS63188B1 (hr) |
| SI (1) | SI2966984T1 (hr) |
| UA (1) | UA121099C2 (hr) |
| WO (1) | WO2014144987A2 (hr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2966984B1 (en) | 2013-03-15 | 2022-03-02 | Cibus US LLC | Targeted gene modification using oligonucleotide-mediated gene repair |
| EP3325668B1 (en) * | 2015-07-23 | 2021-01-06 | Mayo Foundation for Medical Education and Research | Editing mitochondrial dna |
| US11499158B2 (en) | 2016-05-13 | 2022-11-15 | Kaneka Corporation | Method for modifying plant |
| CN109153988B (zh) | 2016-05-13 | 2023-04-18 | 株式会社钟化 | 植物的基因组编辑方法 |
| CN109152343A (zh) | 2016-05-13 | 2019-01-04 | 株式会社钟化 | 转化植物的制备方法 |
| CN106755067A (zh) * | 2016-12-05 | 2017-05-31 | 天津吉诺沃生物科技有限公司 | 通过瞬时表达对植物基因进行定点***的方法及获得的瞬时表达细胞、组织和突变植株 |
| US11859219B1 (en) | 2016-12-30 | 2024-01-02 | Flagship Pioneering Innovations V, Inc. | Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US5100792A (en) | 1984-11-13 | 1992-03-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues |
| US5024944A (en) | 1986-08-04 | 1991-06-18 | Lubrizol Genetics, Inc. | Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species |
| US5008200A (en) | 1988-05-08 | 1991-04-16 | United Agriseeds, Inc. | Propagating multiple whole fertile plants from immature leguminous |
| US5302523A (en) | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
| US5550318A (en) | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| US5204253A (en) | 1990-05-29 | 1993-04-20 | E. I. Du Pont De Nemours And Company | Method and apparatus for introducing biological substances into living cells |
| DE4216134A1 (de) | 1991-06-20 | 1992-12-24 | Europ Lab Molekularbiolog | Synthetische katalytische oligonukleotidstrukturen |
| IT230274Y1 (it) | 1993-06-11 | 1999-06-02 | Silc Spa | Pannolone assorbente sagomato per incontinenza |
| EP0733059B1 (en) | 1993-12-09 | 2000-09-13 | Thomas Jefferson University | Compounds and methods for site-directed mutations in eukaryotic cells |
| ES2203635T3 (es) | 1994-04-27 | 2004-04-16 | Novartis Ag | Nucleosidos y oligonucleotidos con grupos 2'-eter. |
| US5780296A (en) | 1995-01-17 | 1998-07-14 | Thomas Jefferson University | Compositions and methods to promote homologous recombination in eukaryotic cells and organisms |
| US5731181A (en) | 1996-06-17 | 1998-03-24 | Thomas Jefferson University | Chimeric mutational vectors having non-natural nucleotides |
| US5760012A (en) | 1996-05-01 | 1998-06-02 | Thomas Jefferson University | Methods and compounds for curing diseases caused by mutations |
| US5888983A (en) | 1996-05-01 | 1999-03-30 | Thomas Jefferson University | Method and oligonucleobase compounds for curing diseases caused by mutations |
| US6524613B1 (en) | 1997-04-30 | 2003-02-25 | Regents Of The University Of Minnesota | Hepatocellular chimeraplasty |
| JP2002511851A (ja) | 1997-04-30 | 2002-04-16 | リージェンツ オブ ジ ユニバーシティー オブ ミネソタ | 肝細胞の遺伝的病変を修正するためのリコンビナジェニックオリゴヌクレオ塩基のｉｎ ｖｉｖｏでの使用 |
| KR20010022652A (ko) | 1997-08-05 | 2001-03-26 | 키메라젠, 인크. | 식물에서 국소적인 유전자변형을 유발하기 위한 혼성 이중나선올리고뉴클레오티드의 용도 |
| US6004804A (en) | 1998-05-12 | 1999-12-21 | Kimeragen, Inc. | Non-chimeric mutational vectors |
| US6010907A (en) | 1998-05-12 | 2000-01-04 | Kimeragen, Inc. | Eukaryotic use of non-chimeric mutational vectors |
| US6271360B1 (en) | 1999-08-27 | 2001-08-07 | Valigen (Us), Inc. | Single-stranded oligodeoxynucleotide mutational vectors |
| AR025996A1 (es) | 1999-10-07 | 2002-12-26 | Valigen Us Inc | Plantas no transgenicas resistentes a los herbicidas. |
| EP1152058A1 (en) * | 2000-05-03 | 2001-11-07 | Institut Curie | Methods and compositions for effecting homologous recombination |
| AU2002322435A1 (en) | 2001-08-09 | 2003-02-24 | Cibus Genetics | Non-transgenic herbicide resistant plants |
| EP3584325A1 (en) | 2007-10-05 | 2019-12-25 | Cibus Europe B.V. | Mutated acetohydroxyacid synthase genes in brassica |
| HUE025914T2 (en) * | 2007-12-21 | 2016-04-28 | Keygene Nv | Enhanced Mutagenesis Method Using Mutagenic Nucleobases Using Plant Polypropylene Polyethylene Glycol |
| JP2011518555A (ja) * | 2008-04-14 | 2011-06-30 | サンガモ バイオサイエンシーズ， インコーポレイテッド | 標的組込みのための線形ドナーコンストラクト |
| WO2011078662A1 (en) | 2009-12-21 | 2011-06-30 | Keygene N.V. | Dsrna for improved genetic modification of plant dna |
| JP2013534417A (ja) * | 2010-06-14 | 2013-09-05 | アイオワ ステート ユニバーシティ リサーチ ファウンデーション，インコーポレーティッド | Ｔａｌエフェクターとｆｏｋｉの融合タンパク質のヌクレアーゼ活性 |
| JP2013537410A (ja) * | 2010-07-23 | 2013-10-03 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | 標的化エンドヌクレアーゼおよび一本鎖核酸を用いたゲノム編集 |
| SG194089A1 (en) * | 2011-04-27 | 2013-11-29 | Amyris Inc | Methods for genomic modification |
| CA2841165A1 (en) * | 2011-07-11 | 2013-01-17 | Cellular Dynamics International, Inc. | Methods for cell reprogramming and genome engineering |
| EP4289948A3 (en) * | 2012-05-25 | 2024-04-17 | The Regents of the University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| EP2966984B1 (en) | 2013-03-15 | 2022-03-02 | Cibus US LLC | Targeted gene modification using oligonucleotide-mediated gene repair |
| US9680858B1 (en) | 2013-09-09 | 2017-06-13 | BitSight Technologies, Inc. | Annotation platform for a security risk system |
-
2014
- 2014-03-14 EP EP14763072.7A patent/EP2966984B1/en active Active
- 2014-03-14 LT LTEPPCT/US2014/029621T patent/LT2966984T/lt unknown
- 2014-03-14 PT PT147630727T patent/PT2966984T/pt unknown
- 2014-03-14 HR HRP20220521TT patent/HRP20220521T1/hr unknown
- 2014-03-14 KR KR1020157026632A patent/KR102304487B1/ko active IP Right Grant
- 2014-03-14 US US14/777,410 patent/US11434494B2/en active Active
- 2014-03-14 CN CN201480024708.9A patent/CN105338805A/zh active Pending
- 2014-03-14 WO PCT/US2014/029621 patent/WO2014144987A2/en active Application Filing
- 2014-03-14 EA EA201591447A patent/EA201591447A1/ru unknown
- 2014-03-14 AU AU2014233465A patent/AU2014233465A1/en not_active Abandoned
- 2014-03-14 RS RS20220373A patent/RS63188B1/sr unknown
- 2014-03-14 HU HUE14763072A patent/HUE058351T2/hu unknown
- 2014-03-14 MY MYPI2015703120A patent/MY191390A/en unknown
- 2014-03-14 SI SI201431952T patent/SI2966984T1/sl unknown
- 2014-03-14 CA CA2905135A patent/CA2905135C/en active Active
- 2014-03-14 BR BR112015022848-8A patent/BR112015022848B1/pt active IP Right Grant
- 2014-03-14 ES ES14763072T patent/ES2912400T3/es active Active
- 2014-03-14 UA UAA201508559A patent/UA121099C2/uk unknown
- 2014-03-14 JP JP2016503169A patent/JP2016516408A/ja active Pending
- 2014-03-14 DK DK14763072.7T patent/DK2966984T3/da active
- 2014-03-14 IL IL241145A patent/IL241145B/en unknown
- 2014-03-14 PL PL14763072T patent/PL2966984T3/pl unknown
-
2015
- 2015-09-11 CL CL2015002650A patent/CL2015002650A1/es unknown
-
2016
- 2016-07-21 HK HK16108753.2A patent/HK1220864A1/zh unknown
-
2020
- 2020-08-17 AU AU2020220059A patent/AU2020220059B2/en active Active
-
2022
- 2022-09-05 US US17/902,970 patent/US20230141977A1/en active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HRP20220521T1 (hr) | Ciljana genska modifikacija primjenom popravka gena posredovanog oligonukleotidom | |
| JP6668236B2 (ja) | Ｌｅｐｔｉｎｏｔａｒｓａ防除用組成物及びその方法 | |
| Hao et al. | Insights into salicylic acid responses in cucumber (Cucumis sativus L.) cotyledons based on a comparative proteomic analysis | |
| IL307456A (en) | Methods and compositions for increasing the efficiency of targeted gene modification using oligonucleotide-mediated gene repair | |
| Liu et al. | Temporary effect of postharvest UV-C irradiation on gene expression profile in tomato fruit | |
| Lester et al. | Organically versus conventionally grown produce: common production inputs, nutritional quality, and nitrogen delivery between the two systems | |
| RU2014135335A (ru) | Синтетические происходящие из brassica транзитные пептиды хлоропластов | |
| CN108064288B (zh) | 用于控制叶甲属的组合物和方法 | |
| RU2018109099A (ru) | Транзитные пептиды хлоропластов | |
| Wu et al. | Validation of reference genes for RT-qPCR studies of gene expression in preharvest and postharvest longan fruits under different experimental conditions | |
| Rezapour et al. | Effect of seed size on germination and seed vigor of two soybean (Glycine max L.) cultivars | |
| Duran-Flores et al. | Growth inhibition by self-DNA: a phenomenon and its multiple explanations | |
| RU2014145864A (ru) | Синтетические транзитные пептиды хлоропласта из brassica | |
| Rasheed et al. | Allelic variation and composition of HMW-GS in advanced lines derived from D-genome synthetic hexaploid/bread wheat (Triticum aestivum L.) | |
| Pental | When scientists turn against science | |
| Sharma et al. | Genome editing for improving nutritional quality, post-harvest shelf life and stress tolerance of fruits, vegetables, and ornamentals | |
| Fuller et al. | Effect of osmotic potential of activator solution and temperature on viability and vigour of wheat seed | |
| Vishwanath et al. | Chemical tests for identification and characterization of tomato cultivars | |
| MUNJAL | Genetic diversity in bread wheat for heat tolerance | |
| Kumar et al. | Evaluation for fruit production and quality of cherry tomato (Solanum lycopersicum L. var. cerasiforme) | |
| Anh et al. | Effect of salt on growth of rice landraces in Vietnam | |
| Prasantha et al. | Effect of hermetic storage on end-use quality of mungbean | |
| Mafakheri et al. | Abiotic stress in plants: socio-economic consequences and crops responses | |
| Priti et al. | Nutritional Enhancement in Horticultural Crops by CRISPR/Cas9: Status and Future Prospects | |
| Pandey et al. | Dynamics of ‘Candidatus Liberibacter asiaticus’ growth, concentrations of reactive oxygen species, and ion leakage in huanglongbing-positive sweet orange |