HRP20211745T1 - Postupci za dobivanje jednolančane rnk - Google Patents
Postupci za dobivanje jednolančane rnk Download PDFInfo
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- HRP20211745T1 HRP20211745T1 HRP20211745TT HRP20211745T HRP20211745T1 HR P20211745 T1 HRP20211745 T1 HR P20211745T1 HR P20211745T T HRP20211745T T HR P20211745TT HR P20211745 T HRP20211745 T HR P20211745T HR P20211745 T1 HRP20211745 T1 HR P20211745T1
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- 238000000034 method Methods 0.000 title claims 16
- 239000000463 material Substances 0.000 claims 52
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 42
- 239000000872 buffer Substances 0.000 claims 39
- 238000002360 preparation method Methods 0.000 claims 21
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 20
- 150000003839 salts Chemical class 0.000 claims 12
- 238000002156 mixing Methods 0.000 claims 10
- 229920002678 cellulose Polymers 0.000 claims 9
- 239000001913 cellulose Substances 0.000 claims 9
- 239000000203 mixture Substances 0.000 claims 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 8
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 8
- 239000007791 liquid phase Substances 0.000 claims 7
- 238000001914 filtration Methods 0.000 claims 6
- 230000005484 gravity Effects 0.000 claims 6
- 239000007790 solid phase Substances 0.000 claims 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 4
- 239000012928 buffer substance Substances 0.000 claims 4
- 239000002738 chelating agent Substances 0.000 claims 4
- 239000007788 liquid Substances 0.000 claims 4
- 239000011780 sodium chloride Substances 0.000 claims 4
- 238000005406 washing Methods 0.000 claims 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 4
- 238000000926 separation method Methods 0.000 claims 3
- 239000000243 solution Substances 0.000 claims 3
- 239000006228 supernatant Substances 0.000 claims 3
- 229920003043 Cellulose fiber Polymers 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- 239000000725 suspension Substances 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 108020005544 Antisense RNA Proteins 0.000 claims 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 1
- 108010065868 RNA polymerase SP6 Proteins 0.000 claims 1
- 108020004459 Small interfering RNA Proteins 0.000 claims 1
- 101710137500 T7 RNA polymerase Proteins 0.000 claims 1
- 238000003916 acid precipitation Methods 0.000 claims 1
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000003184 complementary RNA Substances 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 239000003480 eluent Substances 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 108091070501 miRNA Proteins 0.000 claims 1
- 239000002679 microRNA Substances 0.000 claims 1
- 238000004810 partition chromatography Methods 0.000 claims 1
- 239000002924 silencing RNA Substances 0.000 claims 1
- 238000000108 ultra-filtration Methods 0.000 claims 1
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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Claims (15)
1. Postupak za dobivanje jednolančane RNK (ssRNA), koji obuhvaća:
(i) proizvodnju preparata RNK koji sadrži ssRNA in vitro transkripcijom;
(ii) dovođenje u kontakt preparata RNK sa celuloznim materijalom pod uvjetima koji omogućavaju vezivanje dvolančane RNK (dsRNA) za celulozni materijal, a ne omogućavaju vezivanje ssRNA za celulozni materijal; i
(iii) odvajanje ssRNA od celuloznog materijala pod uvjetima koji omogućavaju vezivanje dsRNA za celulozni materijal, a ne omogućavaju vezivanje ssRNA za celulozni materijal,
gdje je
u koraku (ii) preparat RNK osiguran kao tekućina koja sadrži ssRNA i prvi pufer i/ili je celulozni materijal osiguran kao suspenzija u prvom puferu, pri čemu prvi pufer sadrži vodu, etanol i sol u koncentraciji koja omogućava vezivanje dsRNA za celulozni materijal, a ne omogućava vezivanje ssRNA za celulozni materijal; i
koncentracija etanola u prvom puferu je 14 do 20% (zapr./zapr.), a koncentracija soli u prvom puferu je 15 do 70 mM.
2. Postupak za dobivanje jednolančane RNK (ssRNA), koji obuhvaća:
(i) proizvodnju preparata RNK koji sadrži ssRNA in vitro transkripcijom;
(ii) dovođenje u kontakt preparata RNK sa celuloznim materijalom pod uvjetima koji omogućavaju vezivanje dvolančane RNK (dsRNA) i ssRNA za celulozni materijal; i
(iii) odvajanje ssRNA od celuloznog materijala pod uvjetima koji omogućavaju vezivanje dsRNA za celulozni materijal, a ne omogućavaju vezivanje ssRNA za celulozni materijal,
gdje
korak (iii) obuhvaća:
(1) miješanje celuloznog materijala za koji su vezane dsRNA i ssRNA sa prvim puferom uz mućkanje i/ili miješanje, gdje prvi pufer sadrži vodu, etanol i sol u koncentraciji koja omogućava vezivanje dsRNA za celulozni materijal, a ne omogućava vezivanje ssRNA za celulozni materijal; i
(2) odvajanje tekuće faze koja sadrži ssRNA od celuloznog materijala;
i
koncentracija etanola u prvom puferu je 14 do 20% (zapr./zapr.), a koncentracija soli u prvom puferu je 15 do 70 mM.
3. Postupak prema patentnom zahtjevu 1, gdje korak (ii) obuhvaća miješanje preparata RNK koji sadrži ssRNA sa celuloznim materijalom uz mućkanje i/ili miješanje, poželjno tokom najmanje 5 min, poželjnije tokom najmanje 10 min.
4. Postupak prema patentnom zahtjevu 3, gdje
(a) sol sadržana u prvom puferu je natrij klorid; i/ili
(b) koncentracija etanola u prvom puferu je 14 do 16% (zapr./zapr.); i/ili
(c) koncentracija soli u prvom puferu je 20 do 60 mM; i/ili
(d) prvi pufer dodatno sadrži pufersku supstancu, poželjno tris(hidroksimetil)aminometan (TRIS), i/ili kelirajući agens, poželjno EDTA; i/ili
(e) u koraku (iii)
(α) mješavina preparata RNK, celuloznog materijala i prvog pufera je osigurana u epruveti i korak (iii) obuhvaća (1) primjenu gravitacije ili centrifugalne sile na epruvetu tako da se tekuća i čvrsta faza razdvoje; i (2) ili sakupljanje supernatanta koji sadrži ssRNA ili uklanjanje celuloznog materijala; ili
(β) mješavina preparata RNK, celuloznog materijala i prvog pufera je osigurana u spin-koloni ili uređaju za filtraciju i korak (iii) obuhvaća (1’) primjenu gravitacije, centrifugalne sile, pritiska, ili vakuuma na spin-kolonu ili uređaj za filtraciju tako da se tekuća i čvrsta faza razdvoje; i (2’) sakupljanje propuštene frakcije koja sadrži ssRNA.
5. Postupak prema bilo kojem od patentnih zahtjeva 1, 3 i 4, gdje se koraci (ii) i (iii) ponavljaju jednom ili dva ili više puta, pri čemu se preparat ssRNA dobiven nakon koraka (iii) jednog ciklusa koraka (ii) i (iii) koristi kao preparat RNK u koraku (ii) sljedećeg ciklusa, i u koraku (ii) svakog ciklusa koraka (ii) i (iii) upotrebljava se svjež celulozni materijal.
6. Postupak prema patentnom zahtjevu 2, gdje korak (ii) obuhvaća
(1) miješanje preparata RNK koji sadrži ssRNA sa celuloznim materijalom uz mućkanje i/ili miješanje, poželjno tokom najmanje 5 min, poželjnije tokom najmanje 10 min; i
(2) odvajanje celuloznog materijala za koji su vezane dsRNA i ssRNA od ostatka.
7. Postupak prema patentnom zahtjevu 6, gdje je u koraku (ii) preparat RNK osiguran kao tekućina koja sadrži ssRNA i drugi pufer i/ili je celulozni materijal osiguran kao suspenzija u drugom puferu, gdje drugi pufer sadrži vodu, etanol i sol, poželjno natrij klorid, u koncentraciji koja omogućava vezivanje dsRNA i ssRNA za celulozni materijal.
8. Postupak prema patentnom zahtjevu 7, gdje
(a) koncentracija etanola u drugom puferu je najmanje 35% (zapr./zapr.), poželjno 38 do 42% (zapr./zapr.); i/ili
(b) koncentracija soli u drugom puferu je 15 do 70 mM, poželjno 20 do 60 mM; i/ili
(c) drugi pufer dodatno sadrži pufersku supstancu, poželjno tris(hidroksimetil)aminometan (TRIS), i/ili kelirajući agens, poželjno EDTA; i/ili
(d) u koraku (ii)(2)
(α) mješavina preparata RNK i celuloznog materijala dobivena u koraku (ii)(1) je osigurana u epruveti i korak (ii)(2) obuhvaća (2a) primjenu gravitacije ili centrifugalne sile na epruvetu tako da se tekuća i čvrsta faza razdvoje; i (2b) ili uklanjanje supernatanta ili sakupljanje celuloznog materijala za koji su vezane dsRNA i ssRNA; ili
(β) mješavina preparata RNK i celuloznog materijala dobivena u koraku (ii)(1) je osigurana u spin-koloni ili uređaju za filtraciju i korak (ii)(2) obuhvaća (2a’) primjenu gravitacije, centrifugalne sile, pritiska, ili vakuuma na spin-kolonu ili uređaj za filtraciju tako da se tekuća i čvrsta faza razdvoje; i (2b’) odbacivanje propuštene frakcije; i/ili
(e) korak (ii) dalje obuhvaća (3) dodavanje alikvota drugog pufera u celulozni materijal za koji su vezane dsRNA i ssRNA; (4) inkubaciju dobivene mješavine uz mućkanje i/ili miješanje, poželjno tokom najmanje 5 min, poželjnije tokom najmanje 10 min; i (5) odvajanje celuloznog materijala za koji su vezane dsRNA i ssRNA od tekuće faze; i izborno (6) ponavljanje koraka (3) do (5) jednom ili dva ili više puta.
9. Postupak prema bilo kojem od patentnih zahtjeva 2 i 6 do 8, gdje
(a) u koraku (iii)(1) celulozni materijal za koji su vezane dsRNA i ssRNA se miješa sa prvim puferom uz mućkanje i/ili miješanje tokom najmanje 5 min, poželjno tokom najmanje 10 min; i/ili
(b) sol sadržana u prvom puferu je natrij klorid; i/ili
(c) koncentracija etanola u prvom puferu je 14 do 16% (zapr./zapr.); i/ili
(d) koncentracija soli u prvom puferu je 20 do 60 mM; i/ili
(e) prvi pufer dodatno sadrži pufersku supstancu, poželjno tris(hidroksimetil)aminometan (TRIS), i/ili kelirajući agens, poželjno EDTA; i/ili
(f) u koraku (iii)
(α) mješavina celuloznog materijala i prvog pufera je osigurana u epruveti i korak (iii)(2) obuhvaća (2a) primjenu gravitacije ili centrifugalne sile na epruvetu tako da se tekuća i čvrsta faza razdvoje; i (2b) ili sakupljanje supernatanta koji sadrži ssRNA ili uklanjanje celuloznog materijala; ili
(β) mješavina celuloznog materijala i prvog pufera je osigurana u spin-koloni ili uređaju za filtraciju i korak (iii)(2) obuhvaća (2a’) primjenu gravitacije, centrifugalne sile, pritiska, ili vakuuma na spin-kolonu ili uređaja za filtraciju; i (2b’) sakupljanje propuštene frakcije koja sadrži ssRNA; i/ili
(g) koraci (ii) i (iii) se ponavljaju jednom ili dva ili više puta, pri čemu se preparat ssRNA dobiven nakon koraka (iii) jednog ciklusa koraka (ii) i (iii) upotrebljava kao preparat RNK u koraku (ii) sljedećeg ciklusa, i u koraku (ii) svakog ciklusa koraka (ii) i (iii) koristi se svjež celulozni materijal.
10. Postupak prema patentnom zahtjevu 2, gdje je u koraku (ii) celulozni materijal osiguran u koloni, korak (ii) obuhvaća nanošenje preparata RNK na kolonu pod uvjetima koji omogućavaju vezivanje dsRNA i ssRNA za celulozni materijal, i korak (iii) obuhvaća eluiranje ssRNA sa celuloznog materijala pod uvjetima koji omogućavaju vezivanje dsRNA za celulozni materijal, a ne omogućavaju vezivanje ssRNA za celulozni materijal.
11. Postupak prema patentnom zahtjevu 10, gdje je u koraku (ii) preparat RNK osiguran i nanijet na kolonu kao tekućina koja sadrži ssRNA i drugi pufer, pri čemu drugi pufer sadrži vodu, etanol i sol, poželjno natrij klorid, u koncentraciji koja omogućava vezivanje dsRNA i ssRNA za celulozni materijal.
12. Postupak prema patentnom zahtjevu 11, gdje
(a) koncentracija etanola u drugom puferu je najmanje 35% (zapr./zapr.), poželjno 38 do 42% (zapr./zapr.); i/ili
(b) koncentracija soli u drugom puferu je 15 do 70 mM, poželjno 20 do 60 mM; i/ili
(c) drugi pufer dodatno sadrži pufersku supstancu, poželjno tris(hidroksimetil)aminometan (TRIS), i/ili helirajući agens, poželjno EDTA; i/ili
(d) korak (iii) se provodi uporabom prvog pufera kao eluenta, pri čemu je prvi pufer poželjno prvi pufer definiran u patentnom zahtjevu 9(b), patentnom zahtjevu 9(c), patentnom zahtjevu 9(d), i/ili patentnom zahtjevu 9(e).
13. Postupak prema bilo kojem od patentnih zahtjeva 1 do 12, gdje
(a) preparat RNK se proizvodi uporabom RNK polimeraze izabrane iz grupe koja se sastoji od RNK polimeraza T3, T7 i SP6; i/ili
(b) prije koraka (ii) preparat RNK se podvrgava najmanje jednom tretmanu pred-pročišćavanja, pri čemu najmanje jedan tretman pred-pročišćavanja poželjno uključuje jedno ili više od sljedećeg: precipitaciju nukleinskih kiselina; vezivanje nukleinskih kiselina za magnetne perle; ultrafiltraciju; i degradaciju DNK; i/ili
(c) ssRNA je iRNK ili inhibitorna RNK, kao što je antisens RNK, siRNA, ili miRNA; i/ili
(d) ssRNA ima dužinu od najmanje 2700 nt, poželjno najmanje 3000 nt, poželjnije najmanje 3500 nt, poželjnije najmanje 4500 nt; i/ili
(e) celulozni materijal sadrži celulozna vlakna, poželjno celulozna vlakna odgovarajućeg kvaliteta za uporabu kao reagens za particijsku kromatografiju; i/ili
(f) prije dovođenja u kontakt sa preparatom RNK u koraku (ii), celulozni materijal je osiguran kao oprani celulozni materijal.
14. Postupak prema patentnom zahtjevu 13(f), gdje pranje celuloznog materijala uključuje
(I) miješanje celuloznog materijala sa otopinom za pranje uz mućkanje i/ili miješanje, poželjno tijekom najmanje 5 min; i
(II) ili uklanjanje tekućine ili sakupljanje celuloznog materijala; i izborno
(III) ponavljanje koraka (I) i (II) jednom ili dva ili više puta.
15. Postupak prema patentnom zahtjevu 14, gdje
(A) ako se korak (ii) provodi pod uvjetima koji omogućavaju vezivanje dsRNA za oprani celulozni materijal, a ne omogućavaju vezivanje ssRNA za oprani celulozni materijal, otopina za pranje ima sastav prvog pufera definiran u patentnom zahtjevu 1, patentnom zahtjevu 4(a), patentnom zahtjevu 4(b), patentnom zahtjevu 4(c), i/ili patentnom zahtjevu 4(d), ili
(B) ako se korak (ii) provodi pod uvjetima koji omogućavaju vezivanje dsRNA i ssRNA za oprani celulozni materijal, otopina za pranje ima sastav drugog pufera definiran u patentnom zahtjevu 7, patentnom zahtjevu 8(a), patentnom zahtjevu 8(b), i/ili patentnom zahtjevu 8(c).
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP2016059056 | 2016-04-22 | ||
EP17722695.8A EP3445850B1 (en) | 2016-04-22 | 2017-04-19 | Methods for providing single-stranded rna |
PCT/EP2017/059293 WO2017182524A1 (en) | 2016-04-22 | 2017-04-19 | Methods for providing single-stranded rna |
Publications (1)
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EP (2) | EP4008782A1 (hr) |
JP (2) | JP7000343B2 (hr) |
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CN (1) | CN109072232B (hr) |
AU (1) | AU2017251983B2 (hr) |
BR (1) | BR112018069417A2 (hr) |
CA (1) | CA3020481A1 (hr) |
CY (1) | CY1124845T1 (hr) |
DK (1) | DK3445850T3 (hr) |
ES (1) | ES2900272T3 (hr) |
HR (1) | HRP20211745T1 (hr) |
HU (1) | HUE059314T2 (hr) |
IL (1) | IL262304A (hr) |
LT (1) | LT3445850T (hr) |
MA (1) | MA44732B1 (hr) |
MD (1) | MD3445850T2 (hr) |
MX (1) | MX2018012880A (hr) |
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RU (2) | RU2760790C2 (hr) |
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SI (1) | SI3445850T1 (hr) |
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Families Citing this family (48)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2760790C2 (ru) | 2016-04-22 | 2021-11-30 | Бионтэк Рна Фармасьютикалз Гмбх | Способы получения одноцепочечной рнк |
WO2018188730A1 (en) * | 2017-04-11 | 2018-10-18 | Biontech Rna Pharmaceuticals Gmbh | Rna for treatment of autoimmune diseases |
WO2019036685A1 (en) | 2017-08-18 | 2019-02-21 | Modernatx, Inc. | METHODS FOR HPLC ANALYSIS |
CA3149497A1 (en) * | 2019-08-09 | 2021-02-18 | Nutcracker Therapeutics, Inc. | Methods and apparatuses for manufacturing for removing material from a therapeutic composition |
WO2021160346A1 (en) | 2020-02-13 | 2021-08-19 | Institut Pasteur | Nucleic acid vaccine against the sars-cov-2 coronavirus |
CA3174588A1 (en) | 2020-03-30 | 2021-10-07 | BioNTech SE | Rna compositions targeting claudin-18.2 |
EP3896160A1 (en) * | 2020-04-17 | 2021-10-20 | Bia Separations D.O.O. | A method of single-stranded rna purification |
EP3896159A1 (en) * | 2020-04-17 | 2021-10-20 | Bia Separations D.O.O. | A method of single strand rna purification employing an anion exchanger |
EP3896161A1 (en) * | 2020-04-17 | 2021-10-20 | Bia Separations D.O.O. | A method of single-stranded rna purification |
WO2021255297A1 (en) | 2020-06-19 | 2021-12-23 | Etherna Immunotherapies Nv | Rna purification method |
JP2023552678A (ja) | 2020-11-16 | 2023-12-19 | ビオンテック・ソシエタス・エウロパエア | 粒子およびmRNAを含む医薬組成物ならびにそれを調製および貯蔵する方法 |
WO2022218503A1 (en) | 2021-04-12 | 2022-10-20 | BioNTech SE | Lnp compositions comprising rna and methods for preparing, storing and using the same |
TW202237148A (zh) | 2020-11-16 | 2022-10-01 | 德商拜恩迪克公司 | 包含rna之lnp組合物以及製備、儲存及使用彼之方法 |
WO2022162027A2 (en) | 2021-01-27 | 2022-08-04 | Curevac Ag | Method of reducing the immunostimulatory properties of in vitro transcribed rna |
CN115197935A (zh) * | 2021-04-08 | 2022-10-18 | 上海细胞治疗集团有限公司 | 纤维素色谱纯化rna的方法 |
EP4322925A2 (en) | 2021-04-12 | 2024-02-21 | BioNTech SE | Rna compositions comprising a buffer substance and methods for preparing, storing and using the same |
KR20240006575A (ko) | 2021-04-26 | 2024-01-15 | 앵스띠뛰 파스퇴르 | SARS-CoV-2에 대한 사람 중화 모노클로날 항체 및 이의 용도 |
WO2022266038A1 (en) * | 2021-06-14 | 2022-12-22 | 2Seventy Bio, Inc. | Single stranded rna purification methods |
WO2022266389A1 (en) * | 2021-06-17 | 2022-12-22 | Modernatx, Inc. | Alternative rna purification strategies |
CN115505589A (zh) * | 2021-07-27 | 2022-12-23 | 上海兆维科技发展有限公司 | 一种rna的制备方法、合成蛋白质的方法以及转录反应液 |
IL309952A (en) | 2021-07-29 | 2024-03-01 | BioNTech SE | Compositions and methods for treating melanoma |
WO2023030635A1 (en) | 2021-09-02 | 2023-03-09 | BioNTech SE | Potency assay for therapeutic potential of coding nucleic acid |
WO2023036960A1 (en) | 2021-09-10 | 2023-03-16 | BioNTech SE | Lipid-based rna formulations suitable for therapy |
WO2023051926A1 (en) | 2021-09-30 | 2023-04-06 | BioNTech SE | Treatment involving non-immunogenic rna for antigen vaccination and pd-1 axis binding antagonists |
AU2022374004A1 (en) | 2021-10-22 | 2024-05-02 | BioNTech SE | Compositions for administration of different doses of rna |
WO2023073228A1 (en) | 2021-10-29 | 2023-05-04 | CureVac SE | Improved circular rna for expressing therapeutic proteins |
AU2022380858A1 (en) * | 2021-11-08 | 2024-05-30 | Gritstone Bio, Inc. | Self-amplifying rna compositions and methods of use thereof |
WO2023083434A1 (en) | 2021-11-09 | 2023-05-19 | BioNTech SE | Rna encoding peptidoglycan hydrolase and use thereof for treating bacterial infection |
WO2023126053A1 (en) | 2021-12-28 | 2023-07-06 | BioNTech SE | Lipid-based formulations for administration of rna |
AU2023212857A1 (en) | 2022-01-27 | 2024-07-04 | BioNTech SE | Pharmaceutical compositions for delivery of herpes simplex virus antigens and related methods |
US20230279471A1 (en) * | 2022-02-28 | 2023-09-07 | Perkinelmer Health Sciences, Inc. | Methods for separating and detecting double-stranded and single-stranded ribonucleic acid (rna) |
WO2023165681A1 (en) | 2022-03-01 | 2023-09-07 | BioNTech SE | Rna lipid nanoparticles (lnps) comprising a polyoxazoline and/or polyoxazine polymer |
KR20230142248A (ko) | 2022-04-01 | 2023-10-11 | 주식회사 엘지에너지솔루션 | 연성회로기판을 포함하는 파우치형 전지셀 |
WO2023193892A1 (en) | 2022-04-05 | 2023-10-12 | BioNTech SE | Nucleic acid compositions comprising an inorganic polyphosphate and methods for preparing, storing and using the same |
WO2023218431A1 (en) | 2022-05-13 | 2023-11-16 | BioNTech SE | Rna compositions targeting hiv |
CN114921457B (zh) * | 2022-05-16 | 2023-09-29 | 硅羿科技(上海)有限公司 | 一种提取dsRNA的方法 |
WO2023230295A1 (en) | 2022-05-25 | 2023-11-30 | BioNTech SE | Rna compositions for delivery of monkeypox antigens and related methods |
WO2024017479A1 (en) | 2022-07-21 | 2024-01-25 | BioNTech SE | Multifunctional cells transiently expressing an immune receptor and one or more cytokines, their use and methods for their production |
WO2024028325A1 (en) | 2022-08-01 | 2024-02-08 | BioNTech SE | Nucleic acid compositions comprising amphiphilic oligo ethylene glycol (oeg)-conjugated compounds and methods of using such compounds and compositions |
WO2024028445A1 (en) | 2022-08-03 | 2024-02-08 | BioNTech SE | Rna for preventing or treating tuberculosis |
WO2024027910A1 (en) | 2022-08-03 | 2024-02-08 | BioNTech SE | Rna for preventing or treating tuberculosis |
WO2024063789A1 (en) | 2022-09-23 | 2024-03-28 | BioNTech SE | Compositions for delivery of malaria antigens and related methods |
WO2024064934A1 (en) | 2022-09-23 | 2024-03-28 | BioNTech SE | Compositions for delivery of plasmodium csp antigens and related methods |
WO2024063788A1 (en) | 2022-09-23 | 2024-03-28 | BioNTech SE | Compositions for delivery of malaria antigens and related methods |
WO2024064931A1 (en) | 2022-09-23 | 2024-03-28 | BioNTech SE | Compositions for delivery of liver stage antigens and related methods |
WO2024074634A1 (en) | 2022-10-06 | 2024-04-11 | BioNTech SE | Rna compositions targeting claudin-18.2 |
WO2024074211A1 (en) | 2022-10-06 | 2024-04-11 | BioNTech SE | Rna compositions targeting claudin-18.2 |
WO2024141955A1 (en) | 2022-12-28 | 2024-07-04 | BioNTech SE | Rna compositions targeting hiv |
Family Cites Families (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2936240A1 (de) * | 1979-09-07 | 1981-03-26 | Bayer Ag, 51373 Leverkusen | Verfahren zur herstellung bekannter und neuer 6-amino-6-desoxy-2,3-0-isopropyliden-(alpha)-l-sorbofuranose-derivate sowie neue zwischenprodukte des verfahrens |
US4475196A (en) | 1981-03-06 | 1984-10-02 | Zor Clair G | Instrument for locating faults in aircraft passenger reading light and attendant call control system |
US4447233A (en) | 1981-04-10 | 1984-05-08 | Parker-Hannifin Corporation | Medication infusion pump |
US4439196A (en) | 1982-03-18 | 1984-03-27 | Merck & Co., Inc. | Osmotic drug delivery system |
US4522811A (en) | 1982-07-08 | 1985-06-11 | Syntex (U.S.A.) Inc. | Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides |
US4447224A (en) | 1982-09-20 | 1984-05-08 | Infusaid Corporation | Variable flow implantable infusion apparatus |
US4487603A (en) | 1982-11-26 | 1984-12-11 | Cordis Corporation | Implantable microinfusion pump system |
US4486194A (en) | 1983-06-08 | 1984-12-04 | James Ferrara | Therapeutic device for administering medicaments through the skin |
US4596556A (en) | 1985-03-25 | 1986-06-24 | Bioject, Inc. | Hypodermic injection apparatus |
US5374548A (en) | 1986-05-02 | 1994-12-20 | Genentech, Inc. | Methods and compositions for the attachment of proteins to liposomes using a glycophospholipid anchor |
MX9203291A (es) | 1985-06-26 | 1992-08-01 | Liposome Co Inc | Metodo para acoplamiento de liposomas. |
US4941880A (en) | 1987-06-19 | 1990-07-17 | Bioject, Inc. | Pre-filled ampule and non-invasive hypodermic injection device assembly |
US4790824A (en) | 1987-06-19 | 1988-12-13 | Bioject, Inc. | Non-invasive hypodermic injection device |
NL8900725A (nl) * | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
US5108921A (en) | 1989-04-03 | 1992-04-28 | Purdue Research Foundation | Method for enhanced transmembrane transport of exogenous molecules |
US5064413A (en) | 1989-11-09 | 1991-11-12 | Bioject, Inc. | Needleless hypodermic injection device |
US5312335A (en) | 1989-11-09 | 1994-05-17 | Bioject Inc. | Needleless hypodermic injection device |
US5383851A (en) | 1992-07-24 | 1995-01-24 | Bioject Inc. | Needleless hypodermic injection device |
IL151928A0 (en) | 2000-03-30 | 2003-04-10 | Whitehead Biomedical Inst | Rna sequence-specific mediators of rna interference |
JP3692395B2 (ja) * | 2000-09-01 | 2005-09-07 | 独立行政法人農業・生物系特定産業技術研究機構 | ベクターモノカリオンを用いた紫紋羽病菌に対するゲノムが2本鎖rnaである糸状菌に寄生するウイルスを導入する新規方法 |
WO2005012487A2 (en) * | 2003-08-01 | 2005-02-10 | Invitrogen Corporation | Compositions and methods for preparing short rna molecules and other nucleic acids |
DE102005046490A1 (de) | 2005-09-28 | 2007-03-29 | Johannes-Gutenberg-Universität Mainz | Modifikationen von RNA, die zu einer erhöhten Transkriptstabilität und Translationseffizienz führen |
CN101086011B (zh) * | 2006-06-08 | 2010-12-08 | 河南农业大学 | 食用菌和植物双链rna病毒检测试剂盒及其应用 |
DE102006061015A1 (de) | 2006-12-22 | 2008-06-26 | Curevac Gmbh | Verfahren zur Reinigung von RNA im präparativen Maßstab mittels HPLC |
PT2167523E (pt) | 2007-06-19 | 2014-09-22 | Univ Louisiana State | Síntese e utilização de análogos fosforotiolato antireversos do capuz de arn mensageiro |
US8808982B2 (en) | 2009-12-07 | 2014-08-19 | Cellscript, Llc | Compositions and methods for reprogramming eukaryotic cells |
DK3421601T3 (da) | 2011-12-30 | 2020-02-24 | Cellscript Llc | Fremstilling og anvendelse af in-vitro-syntetiseret ssRNA til indføring i pattedyrceller med henblik på at inducere en biologisk eller biokemisk effekt |
EP3744843A1 (en) * | 2015-05-29 | 2020-12-02 | CureVac Real Estate GmbH | A method for producing and purifying rna, comprising at least one step of tangential flow filtration |
RU2760790C2 (ru) | 2016-04-22 | 2021-11-30 | Бионтэк Рна Фармасьютикалз Гмбх | Способы получения одноцепочечной рнк |
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