HRP20130921T1 - Staniäśni klon koji proizvodi fsh - Google Patents
Staniäśni klon koji proizvodi fsh Download PDFInfo
- Publication number
- HRP20130921T1 HRP20130921T1 HRP20130921AT HRP20130921T HRP20130921T1 HR P20130921 T1 HRP20130921 T1 HR P20130921T1 HR P20130921A T HRP20130921A T HR P20130921AT HR P20130921 T HRP20130921 T HR P20130921T HR P20130921 T1 HRP20130921 T1 HR P20130921T1
- Authority
- HR
- Croatia
- Prior art keywords
- nucleic acid
- acid sequence
- seq
- recombinant
- acid molecule
- Prior art date
Links
- 210000004368 gonadotroph Anatomy 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 claims 44
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 27
- 108020004707 nucleic acids Proteins 0.000 claims 17
- 102000039446 nucleic acids Human genes 0.000 claims 17
- 210000004027 cell Anatomy 0.000 claims 11
- 108091026890 Coding region Proteins 0.000 claims 9
- 102000003864 Human Follicle Stimulating Hormone Human genes 0.000 claims 6
- 108010082302 Human Follicle Stimulating Hormone Proteins 0.000 claims 6
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 claims 5
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 claims 5
- 229940028334 follicle stimulating hormone Drugs 0.000 claims 5
- 238000004113 cell culture Methods 0.000 claims 4
- 238000000034 method Methods 0.000 claims 4
- 239000012228 culture supernatant Substances 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 210000002966 serum Anatomy 0.000 claims 2
- 238000004114 suspension culture Methods 0.000 claims 2
- 238000001890 transfection Methods 0.000 claims 2
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Reproductive Health (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Claims (20)
1. Molekula nukleinske kiseline koja obuhvaća sekvencu nukleinskih kiselina koja kodira β lanac humanog folikulo stimulirajućeg hormona (FSH), a to je kodirajuća regija sekvence nukleinskih kiselina prema SEQ ID No. 1.
2. Rekombinantna molekula nukleinske kiseline koja ima prvu sekvencu nukleinskih kiselina prema zahtjevu 1 pod kontrolom promotora koji je aktivan u stanici domaćinu.
3. Rekombinantna molekula nukleinske kiseline prema zahtjevu 2, dalje ima drugu sekvencu nukleinskih kiselina koja kodira α lanac humanog folikulo stimulirajućeg hormona (FSH), a izabire se iz grupe koja obuhvaća kodirajuću regiju sekvence nukleinskih kiselina prema SEQ ID No. 2 i sekvencu nukleinskih kiselina čija je jednakost sekvence najmanje 85% u odnosu na kodirajuću regiju sekvence nukleinskih kiselina kako je opisano za SEQ ID No. 2.
4. Rekombinantna molekula nukleinske kiseline prema zahtjevu 2, dalje ima drugu sekvencu nukleinskih kiselina koja se izabire iz grupe koja obuhvaća kodirajuću regiju sekvence nukleinskih kiselina prema SEQ ID No. 3 i sekvencu nukleinskih kiselina čija je jednakost sekvence najmanje 70% u odnosu na kodirajuću regiju sekvence nukleinskih kiselina kako je opisano za SEQ ID No. 3.
5. Rekombinantna molekula nukleinske kiseline prema zahtjevu 3 ili 4, gdje je druga sekvenca nukleinskih kiselina pod kontrolom posebnog promotora.
6. Rekombinantna molekula nukleinske kiseline prema bilo kojem od zahtjeva 2 do 5, gdje je prva sekvenca nukleinskih kiselina i/ili druga sekvenca nukleinskih kiselina pod kontrolom virusnog promotora.
7. Rekombinantna molekula nukleinske kiseline prema zahtjevu 6, gdje je prva sekvenca nukleinskih kiselina pod kontrolom SV40 promotora.
8. Rekombinantna molekula nukleinske kiseline prema zahtjevu 6, gdje je druga sekvenca nukleinskih kiselina pod kontrolom CMV promotora.
9. Rekombinantna molekula nukleinske kiseline prema bilo kojem od zahtjeva 2 do 8, ima sekvencu nukleinskih kiselina kako je prikazano za SEQ ID No. 7.
10. Stanica domaćin koja sadrži rekombinantnu molekulu nukleinske kiseline prema bilo kojem od zahtjeva 3 do 9.
11. Stanica domaćin prema zahtjevu 10, koja je stanica sisavaca.
12. Stanica domaćin prema zahtjevu 10 ili 11, koja je stanica jajnika kineskog hrčka (CHO).
13. Stanica domaćin prema bilo kojem od zahtjeva 10 do 12, ima depozitni broj DSM ACC2833.
14. Stanica domaćin koja sadrži prvu rekombinantnu molekulu nukleinske kiseline prema zahtjevu 2 i drugu rekombinantnu molekulu nukleinske kiseline sa sekvencom nukleinskih kiselina koja kodira α lanac humanog folikulo stimulirajućeg hormona (FSH), a izabire se iz grupe koja obuhvaća kodirajuću regiju sekvence nukleinskih kiselina prema SEQ ID No. 2, sekvencu nukleinskih kiselina čija je jednakost sekvence najmanje 85% u odnosu na kodirajuću regiju sekvence nukleinskih kiselina kako je opisano za SEQ ID No. 2 i sekvencu nukleinskih kiselina kako je opisano za SEQ ID No. 3.
15. Stanična kultura koja sadrži stanicu domaćina prema bilo kojem od zahtjeva 10 do 14 u prikladnom kulturnom mediju.
16. Postupak proizvodnje rekombinantnog humanog FSH, koji obuhvaća korake:
- uzgoj kulture stanica domaćina prema bilo kojem od zahtjeva 10 do 14 u prikladnom kulturnom mediju; i
- ubiranje supernatanta stanične kulture.
17. Postupak prema zahtjevu 16, dalje obuhvaća korak pročišćavanja rekombinantnog humaog FSH iz supernatanta stanične kulture.
18. Postupak proizvodnje stanica domaćina prema bilo kojem od zahtjeva 10 do 13, obuhvaća transfekciju stanica, u kulturi suspenzije pod uvjetima bez prisutnosti seruma, rekombinantnom molekulom nukleinske kiseline prema bilo kojem od zahtjeva 3 do 9.
19. Postupak proizvodnje stanica domaćina prema zahtjevu 14, obuhvaća transfekciju stanica, u kulturi suspenzije pod uvjetima bez prisutnosti seruma, prvom rekombinantnom molekulom nukleinske kiseline prema zahtjevu 2 i drugom rekombinantnom molekulom nukleinske kiseline koja obuhvaća sekvencu nukleinskih kiselina koja kodira α lanac humanog folikulo stimulirajućeg hormona (FSH), a izabire se iz grupe koja obuhvaća kodirajuću regiju sekvence nukleinskih kiselina prema SEQ ID No. 2, sekvencu nukleinskih kiselina čija je jednakost sekvence najmanje 85% u odnosu na kodirajuću regiju sekvence nukleinskih kiselina kako je opisano za SEQ ID No. 2 i sekvencu nukleinskih kiselina kako je opisano za SEQ ID No. 3.
20. Uporaba sekvence nukleinskih kiselina prema SEQ ID No. 1 ili obje sekvence nukleinskih kiselina prema SEQ ID No. 1 i 2 za in vitro proizvodnju humanog rekombinantnog FSH.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07111257 | 2007-06-28 | ||
| PCT/EP2008/058274 WO2009000913A1 (en) | 2007-06-28 | 2008-06-27 | Fsh producing cell clone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20130921T1 true HRP20130921T1 (hr) | 2013-11-08 |
Family
ID=39745099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20130921AT HRP20130921T1 (hr) | 2007-06-28 | 2013-09-30 | Staniäśni klon koji proizvodi fsh |
Country Status (24)
| Country | Link |
|---|---|
| US (1) | US8318457B2 (hr) |
| EP (2) | EP2170942B1 (hr) |
| JP (1) | JP5570980B2 (hr) |
| KR (1) | KR101522217B1 (hr) |
| CN (1) | CN101720332B (hr) |
| AU (1) | AU2008267138B9 (hr) |
| BR (1) | BRPI0813913B1 (hr) |
| CA (1) | CA2690844C (hr) |
| CY (1) | CY1114463T1 (hr) |
| DK (1) | DK2170942T3 (hr) |
| EA (1) | EA022993B1 (hr) |
| ES (1) | ES2432183T3 (hr) |
| HK (1) | HK1140782A1 (hr) |
| HR (1) | HRP20130921T1 (hr) |
| IL (1) | IL202280A (hr) |
| MX (1) | MX2010000385A (hr) |
| NZ (1) | NZ581702A (hr) |
| PL (1) | PL2170942T3 (hr) |
| PT (1) | PT2170942E (hr) |
| RS (1) | RS52976B (hr) |
| SI (1) | SI2170942T1 (hr) |
| UA (1) | UA103598C2 (hr) |
| WO (1) | WO2009000913A1 (hr) |
| ZA (1) | ZA200908168B (hr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101573773B1 (ko) | 2008-02-08 | 2015-12-02 | 바이오제너릭스 게엠베하 | Ｆｓｈ의 액체 포뮬레이션 |
| JP5845170B2 (ja) | 2009-04-01 | 2016-01-20 | ラティオファーム ゲーエムベーハー | 組換えｆｓｈを精製する方法 |
| EP2325194A1 (en) | 2009-11-24 | 2011-05-25 | Glycotope GmbH | Process for the purification of glycoproteins |
| JP6279466B2 (ja) * | 2012-04-27 | 2018-02-14 | Ｊｃｒファーマ株式会社 | 新規な発現ベクター |
| CN103539861B (zh) * | 2013-11-01 | 2015-02-18 | 广州联康生物科技有限公司 | 长效重组人促卵泡激素融合蛋白 |
| CN103554269B (zh) * | 2013-11-01 | 2015-02-11 | 广州联康生物科技有限公司 | 重组猪促卵泡激素融合蛋白 |
| CN103539860B (zh) * | 2013-11-01 | 2014-12-03 | 广州优联康医药科技有限公司 | 一种长效重组人促卵泡激素融合蛋白 |
| CN105462909B (zh) * | 2015-12-31 | 2019-09-27 | 哈药集团技术中心 | 一种高效表达人促卵泡激素的cho细胞的培养方法 |
| CN107460206B (zh) * | 2017-09-11 | 2020-05-01 | 深圳市深研生物科技有限公司 | 人fsh的重组表达载体、重组细胞株及重组人fsh的制备方法 |
| CN111349154A (zh) * | 2018-12-21 | 2020-06-30 | 江苏众红生物工程创药研究院有限公司 | 一种重组人促卵泡激素及其制备方法和药物应用 |
| CN110812366B (zh) * | 2019-11-18 | 2023-11-17 | 珠海丽凡达生物技术有限公司 | 一种可用于激素补充的mRNA药物及其制备方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4923805A (en) | 1983-11-02 | 1990-05-08 | Integrated Genetics, Inc. | Fsh |
| IT1206302B (it) | 1987-06-26 | 1989-04-14 | Serono Cesare Ist Ricerca | Ormone follicolo-stimolante urinario |
| CA2018676C (en) * | 1989-06-20 | 2003-05-06 | Christie A. Kelton | Novel heteropolymeric protein production methods |
| EP0842275A1 (en) | 1995-02-17 | 1998-05-20 | Instituut Voor Dierhouderij En Diergezondheid (Id-Dlo) | PRODUCTION OF BIOLOGICALLY ACTIVE RECOMBINANT BOVINE FOLLICLE STIMULATING HORMONE (REC bFSH) IN THE BACULOVIRUS EXPRESSION SYSTEM |
| IL122732A0 (en) | 1997-01-15 | 1998-08-16 | Akzo Nobel Nv | Liquid gonadotropin-containing formulation its preparation and a device containing same |
| AU776823B2 (en) | 1998-07-23 | 2004-09-23 | Ares Trading S.A. | FSH and FSH variant formulations, products and methods |
| AU779005B2 (en) | 1999-04-16 | 2004-12-23 | Instituto Massone S.A. | Highly purified gonadotropin compositions and process for preparing them |
| WO2001058493A1 (en) | 2000-02-11 | 2001-08-16 | Maxygen Aps | Conjugates of follicle stimulating hormones |
| US20020127652A1 (en) | 2000-02-11 | 2002-09-12 | Schambye Hans Thalsgard | Follicle stimulating hormones |
| AU2003258388C1 (en) * | 2002-09-13 | 2010-03-18 | The University Of Queensland | Gene expression system based on codon translation efficiency |
| KR100533794B1 (ko) * | 2003-10-02 | 2005-12-07 | 주식회사 프로젠 | 인간 난포자극 호르몬을 대량으로 생산하는 방법 |
| EA008924B1 (ru) | 2003-12-22 | 2007-08-31 | Арес Трейдинг С.А. | Способ очистки фсг |
| MX2007005327A (es) | 2004-11-09 | 2007-08-02 | Ares Trading Sa | Metodo para purificacion de la hormona foliculo estimulante (hfe). |
| TWI369401B (en) * | 2005-07-05 | 2012-08-01 | Ares Trading Sa | Serum-free culture medium for the production of recombinant gonadotropins |
| US20070281883A1 (en) | 2006-06-05 | 2007-12-06 | Hanna Rosenfeld | Development of follicle stimulating hormone agonists and antagonists in fish |
-
2008
- 2008-06-27 UA UAA201000813A patent/UA103598C2/uk unknown
- 2008-06-27 AU AU2008267138A patent/AU2008267138B9/en active Active
- 2008-06-27 EP EP08774439.7A patent/EP2170942B1/en active Active
- 2008-06-27 RS RS20130443A patent/RS52976B/en unknown
- 2008-06-27 PL PL08774439T patent/PL2170942T3/pl unknown
- 2008-06-27 ES ES08774439T patent/ES2432183T3/es active Active
- 2008-06-27 CN CN200880022358.7A patent/CN101720332B/zh active Active
- 2008-06-27 CA CA2690844A patent/CA2690844C/en not_active Expired - Fee Related
- 2008-06-27 EA EA201070071A patent/EA022993B1/ru not_active IP Right Cessation
- 2008-06-27 JP JP2010513938A patent/JP5570980B2/ja not_active Expired - Fee Related
- 2008-06-27 DK DK08774439.7T patent/DK2170942T3/da active
- 2008-06-27 NZ NZ581702A patent/NZ581702A/en not_active IP Right Cessation
- 2008-06-27 US US12/666,257 patent/US8318457B2/en not_active Expired - Fee Related
- 2008-06-27 SI SI200831065T patent/SI2170942T1/sl unknown
- 2008-06-27 WO PCT/EP2008/058274 patent/WO2009000913A1/en active Application Filing
- 2008-06-27 EP EP12169533A patent/EP2492280A1/en not_active Withdrawn
- 2008-06-27 PT PT87744397T patent/PT2170942E/pt unknown
- 2008-06-27 MX MX2010000385A patent/MX2010000385A/es active IP Right Grant
- 2008-06-27 KR KR1020107001959A patent/KR101522217B1/ko active IP Right Grant
- 2008-06-27 BR BRPI0813913-0A patent/BRPI0813913B1/pt active IP Right Grant
-
2009
- 2009-11-20 ZA ZA200908168A patent/ZA200908168B/xx unknown
- 2009-11-23 IL IL202280A patent/IL202280A/en active IP Right Grant
-
2010
- 2010-07-27 HK HK10107184.9A patent/HK1140782A1/xx not_active IP Right Cessation
-
2013
- 2013-09-30 HR HRP20130921AT patent/HRP20130921T1/hr unknown
- 2013-10-09 CY CY20131100874T patent/CY1114463T1/el unknown
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