HK1179304A1 - Method and compositions for detection and enumeration of genetic variations - Google Patents

Method and compositions for detection and enumeration of genetic variations

Info

Publication number
HK1179304A1
HK1179304A1 HK13106839.7A HK13106839A HK1179304A1 HK 1179304 A1 HK1179304 A1 HK 1179304A1 HK 13106839 A HK13106839 A HK 13106839A HK 1179304 A1 HK1179304 A1 HK 1179304A1
Authority
HK
Hong Kong
Prior art keywords
enumeration
compositions
detection
genetic variations
genetic
Prior art date
Application number
HK13106839.7A
Other languages
English (en)
Chinese (zh)
Inventor
Devin Dressman
Hai Yan
Kenneth Kinzler
Bert Vogelstein
Original Assignee
Univ Johns Hopkins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34107717&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HK1179304(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Publication of HK1179304A1 publication Critical patent/HK1179304A1/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK13106839.7A 2003-07-05 2013-06-10 Method and compositions for detection and enumeration of genetic variations HK1179304A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48530103P 2003-07-05 2003-07-05
US52585903P 2003-12-01 2003-12-01

Publications (1)

Publication Number Publication Date
HK1179304A1 true HK1179304A1 (en) 2013-09-27

Family

ID=34107717

Family Applications (1)

Application Number Title Priority Date Filing Date
HK13106839.7A HK1179304A1 (en) 2003-07-05 2013-06-10 Method and compositions for detection and enumeration of genetic variations

Country Status (6)

Country Link
US (4) US8048627B2 (xx)
EP (3) EP2918595B1 (xx)
JP (1) JP5183063B2 (xx)
CA (1) CA2531105C (xx)
HK (1) HK1179304A1 (xx)
WO (1) WO2005010145A2 (xx)

Families Citing this family (418)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
CA2513541A1 (en) 2003-01-29 2004-08-19 454 Corporation Method for preparing single-stranded dna libraries
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
KR20070112785A (ko) 2005-02-01 2007-11-27 에이젠코트 바이오사이언스 코오포레이션 비드-기초 서열화를 위한 시약, 방법, 및 라이브러리
EP2233582A1 (en) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
PL1712639T3 (pl) 2005-04-06 2009-02-27 Maurice Stroun Sposób diagnozowania nowotworu przez wykrywanie krążącego DNA i RNA
EP1760159A1 (en) * 2005-08-31 2007-03-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. A method of electrophoresis of nucleic acid molecules
EP2428579B1 (en) * 2005-10-24 2013-05-29 The Johns Hopkins University Improved methods for BEAMing
US20100137163A1 (en) 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
SI1981995T2 (sl) 2006-02-02 2019-08-30 The Board Of Trustees Of The Leland Stanford Junior University Neinvazivni genetski presejalni test ploda z digitalno analizo
US8460878B2 (en) 2006-02-21 2013-06-11 The Trustees Of Tufts College Methods and arrays for detecting cells and cellular components in small defined volumes
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US7682791B2 (en) * 2006-10-29 2010-03-23 Macevicz Stephen C Method of generating nested sets of double stranded DNA circles
WO2008076842A2 (en) * 2006-12-14 2008-06-26 Applied Biosystems Inc. Sequencing methods
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20090062132A1 (en) 2007-08-29 2009-03-05 Borner Scott R Alternative nucleic acid sequencing methods
EP2201374B1 (en) 2007-08-30 2015-10-07 Trustees Of Tufts College Methods for determining the concentration of an analyte in solution.
WO2009085215A1 (en) 2007-12-21 2009-07-09 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
EP2274441B1 (en) 2008-02-04 2012-06-20 Life Technologies Corporation Composition and method for sequencing nucleic acid
CA2717320A1 (en) * 2008-03-11 2009-09-17 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
EP2262911B1 (en) * 2008-04-17 2016-10-12 QIAGEN Gaithersburg, Inc. Compositions, methods, and kits using synthetic probes for determining the presence of a target nucleic acid
EP2291533B2 (en) 2008-07-02 2020-09-30 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
EP3103871B1 (en) 2008-09-16 2020-07-29 Sequenom, Inc. Processes for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for fetal nucleic acid quantification
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US20110159499A1 (en) * 2009-11-25 2011-06-30 Quantalife, Inc. Methods and compositions for detecting genetic material
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9127312B2 (en) 2011-02-09 2015-09-08 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
EP2340314B8 (en) 2008-10-22 2015-02-18 Illumina, Inc. Preservation of information related to genomic dna methylation
CA2741650C (en) 2008-10-27 2017-09-05 Qiagen Gaithersburg Inc. Fast results hybrid capture assay and system
CA2742298C (en) 2008-11-03 2019-09-10 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
EP2364368B1 (en) 2008-11-07 2014-01-15 Sequenta, Inc. Methods of monitoring conditions by sequence analysis
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
WO2010080134A1 (en) 2008-12-19 2010-07-15 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
US8236532B2 (en) 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
WO2010083456A1 (en) 2009-01-15 2010-07-22 Imdaptive Inc. Adaptive immunity profiling and methods for generation of monoclonal antibodies
US8741558B2 (en) 2009-02-04 2014-06-03 Wake Forest University Compositions, methods, and kits for identifying candidate molecules from encoded chemical libraries
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
WO2010104597A2 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Scale-up of microfluidic devices
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
EP3327139A1 (en) * 2009-04-06 2018-05-30 The Johns Hopkins University Digital quantification of dna methylation
CA2760542A1 (en) * 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
RU2539032C2 (ru) 2009-06-25 2015-01-10 Фред Хатчинсон Кансэр Рисёч Сентер Способ измерения искусственного иммунитета
CN102574078B (zh) 2009-09-02 2016-05-18 哈佛学院院长等 使用喷射和其它技术产生的多重乳液
EP2940153B1 (en) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US20110053143A1 (en) * 2009-09-03 2011-03-03 Qiagen Gaithersburg Inc. Methods and kits for determining viral load in clinical samples
US9410146B2 (en) * 2009-09-14 2016-08-09 Qiagen Gaithersburg Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
CN102648053B (zh) 2009-10-27 2016-04-27 哈佛学院院长等 液滴生成技术
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CA2785020C (en) 2009-12-22 2020-08-25 Sequenom, Inc. Processes and kits for identifying aneuploidy
CA2786322A1 (en) * 2010-01-04 2011-07-07 Qiagen Gaithersburg, Inc. Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples
EP2513341B1 (en) 2010-01-19 2017-04-12 Verinata Health, Inc Identification of polymorphic sequences in mixtures of genomic dna by whole genome sequencing
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
CN102822189B (zh) * 2010-01-29 2016-06-29 奇亚根盖瑟斯堡股份有限公司 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物
EP2531880B1 (en) 2010-02-01 2016-08-03 Illumina Inc. Focusing methods and optical systems and assemblies using the same
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US8535889B2 (en) 2010-02-12 2013-09-17 Raindance Technologies, Inc. Digital analyte analysis
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
JP5363663B2 (ja) 2010-03-01 2013-12-11 クワンテリクス コーポレーション 分子または粒子を検出するアッセイにおけるダイナミックレンジを拡張するための方法またはシステム
US8481903B2 (en) 2010-03-06 2013-07-09 Alexander Triener Systems, methods, and apparatuses including a moveable optical component for detecting optical signals from a sample
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
JP6155419B2 (ja) 2010-03-25 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 検出用の液滴輸送システム
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
EP2556171B1 (en) 2010-04-05 2015-09-02 Prognosys Biosciences, Inc. Spatially encoded biological assays
EP2572001A2 (en) 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
WO2011156387A2 (en) 2010-06-07 2011-12-15 Esoterix Genetic Laboratories, Llc Enumeration of nucleic acids
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
EP2596127A2 (en) 2010-07-23 2013-05-29 Esoterix Genetic Laboratories, LLC Identification of differentially represented fetal or maternal genomic regions and uses thereof
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
US8753816B2 (en) 2010-10-26 2014-06-17 Illumina, Inc. Sequencing methods
US20120135414A1 (en) 2010-10-28 2012-05-31 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
CN106268390B (zh) 2010-11-01 2020-01-10 伯乐生命医学产品有限公司 用于形成乳液的***
WO2012074855A2 (en) 2010-11-22 2012-06-07 The Regents Of The University Of California Methods of identifying a cellular nascent rna transcript
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
EP2673614B1 (en) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Method for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
EP2686449B1 (en) 2011-03-18 2020-11-18 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
WO2012142301A2 (en) 2011-04-12 2012-10-18 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012249759A1 (en) 2011-04-25 2013-11-07 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US8460872B2 (en) 2011-04-29 2013-06-11 Sequenom, Inc. Quantification of a minority nucleic acid species
EP2714254B1 (en) 2011-05-23 2017-09-06 President and Fellows of Harvard College Control of emulsions, including multiple emulsions
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
US20140235474A1 (en) 2011-06-24 2014-08-21 Sequenom, Inc. Methods and processes for non invasive assessment of a genetic variation
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
PT3623481T (pt) 2011-09-23 2021-10-15 Illumina Inc Composições para sequenciação de ácidos nucleicos
AU2012316218B2 (en) 2011-09-26 2016-03-17 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
EP2764459B1 (en) 2011-10-06 2021-06-30 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20140242588A1 (en) 2011-10-06 2014-08-28 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK2766496T3 (en) 2011-10-11 2017-05-15 Sequenom Inc METHODS AND PROCESSES FOR NON-INVASIVE ASSESSMENT OF GENETIC VARIATIONS
CA2853088C (en) 2011-10-21 2018-03-13 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
EP2776165A2 (en) 2011-11-07 2014-09-17 Illumina, Inc. Integrated sequencing apparatuses and methods of use
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
ES2867924T3 (es) 2011-12-09 2021-10-21 Adaptive Biotechnologies Corp Diagnóstico de tumores malignos linfoides y detección de enfermedad residual mínima
WO2013085710A2 (en) 2011-12-09 2013-06-13 Illumina, Inc. Expanded radix for polymeric tags
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
US9746466B2 (en) 2011-12-23 2017-08-29 Nanomedica Llc Integrated compound discovery systems and methods
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
EP2872523B1 (en) 2011-12-30 2018-01-17 Abbott Molecular Inc. Microorganism nucleic acid purification from host samples
PL2805280T3 (pl) 2012-01-20 2022-11-21 Sequenom, Inc. Procesy diagnostyczne będące czynnikiem warunków doświadczalnych
JP6375230B2 (ja) 2012-02-27 2018-08-15 セルラー リサーチ, インコーポレイテッド 分子計数のための組成物およびキット
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
US9670529B2 (en) 2012-02-28 2017-06-06 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
ES2930180T3 (es) 2012-03-02 2022-12-07 Sequenom Inc Métodos para enriquecer ácido nucleico canceroso a partir de una muestra biológica
JP6302847B2 (ja) 2012-03-05 2018-03-28 アダプティヴ バイオテクノロジーズ コーポレーション 頻度が一致したサブユニットからの、対をなす免疫受容体鎖の決定
NO2694769T3 (xx) 2012-03-06 2018-03-03
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
CN204832037U (zh) 2012-04-03 2015-12-02 伊鲁米那股份有限公司 检测设备
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
RU2631797C2 (ru) 2012-05-08 2017-09-26 Эдэптив Байотекнолоджиз Корпорейшн Композиции и способы измерения и калибровки систематической ошибки амплификации в мультиплексных пцр-реакциях
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3978621B1 (en) 2012-05-21 2023-08-30 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20150167084A1 (en) 2012-07-03 2015-06-18 Sloan Kettering Institute For Cancer Research Quantitative Assessment of Human T-Cell Repertoire Recovery After Allogeneic Hematopoietic Stem Cell Transplantation
AU2013290102B2 (en) 2012-07-13 2018-11-15 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR102090851B1 (ko) 2012-08-14 2020-03-19 10엑스 제노믹스, 인크. 마이크로캡슐 조성물 및 방법
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
SG11201501662TA (en) 2012-09-04 2015-05-28 Guardant Health Inc Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP3640343A1 (en) 2012-10-01 2020-04-22 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
CA2887094C (en) 2012-10-04 2021-09-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9644204B2 (en) 2013-02-08 2017-05-09 10X Genomics, Inc. Partitioning and processing of analytes and other species
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
WO2014168711A1 (en) 2013-03-13 2014-10-16 Sequenom, Inc. Primers for dna methylation analysis
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
CN113337604A (zh) 2013-03-15 2021-09-03 莱兰斯坦福初级大学评议会 循环核酸肿瘤标志物的鉴别和用途
WO2014142981A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Enzyme-linked nucleotides
EP2981921B1 (en) 2013-04-03 2023-01-18 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR102540202B1 (ko) 2013-05-24 2023-06-02 시쿼넘, 인코포레이티드 유전적 변이의 비침습 평가를 위한 방법 및 프로세스
CA2915628C (en) 2013-06-21 2020-04-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
MX363806B (es) 2013-07-01 2019-04-03 Illumina Inc Funcionalización de superficies sin catalizador e injerto de polímeros..
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
CN105358715B (zh) 2013-07-03 2018-09-18 伊鲁米那股份有限公司 正交合成测序
MX370560B (es) 2013-08-08 2019-12-17 Illumina Inc Sistema de fluidos para distribución de reactivo a una celda de flujo.
GB2525104B (en) 2013-08-28 2016-09-28 Cellular Res Inc Massively Parallel Single Cell Nucleic Acid Analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
JP6525434B2 (ja) 2013-10-04 2019-06-05 セクエノム, インコーポレイテッド 遺伝子の変異の非侵襲的な評価のための方法および処理
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
JP6680680B2 (ja) 2013-10-07 2020-04-15 セクエノム, インコーポレイテッド 染色体変化の非侵襲性評価のための方法およびプロセス
EP3875601A1 (en) 2013-10-17 2021-09-08 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US10100355B2 (en) 2013-12-09 2018-10-16 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3524694B1 (en) 2013-12-28 2020-07-15 Guardant Health, Inc. Methods and systems for detecting genetic variants
WO2015134787A2 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
EP3117011B1 (en) 2014-03-13 2020-05-06 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
CN106414772B (zh) * 2014-04-08 2021-02-19 华盛顿大学商业中心 用于使用多分散小滴执行数字检定的方法和设备
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
ES2777529T3 (es) 2014-04-17 2020-08-05 Adaptive Biotechnologies Corp Cuantificación de genomas de células inmunitarias adaptativas en una mezcla compleja de células
CN106574305B (zh) 2014-06-13 2021-03-02 生命技术公司 多重核酸扩增
CA2952503A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
KR20230070325A (ko) 2014-06-26 2023-05-22 10엑스 제노믹스, 인크. 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법
US11783911B2 (en) 2014-07-30 2023-10-10 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
EP3653726B1 (en) 2014-08-11 2021-10-27 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
CN107076739B (zh) 2014-08-21 2018-12-25 伊卢米纳剑桥有限公司 可逆表面官能化
SG10201911069WA (en) 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
AU2015318017B2 (en) 2014-09-18 2022-02-03 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
EP3201356A1 (en) 2014-10-03 2017-08-09 Life Technologies Corporation Genetic sequence verification compositions, methods and kits
WO2016057901A1 (en) 2014-10-10 2016-04-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3212790B1 (en) 2014-10-29 2020-03-25 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
CN107002128A (zh) 2014-10-29 2017-08-01 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
LT3212684T (lt) 2014-10-31 2020-04-10 Illumina Cambridge Limited Polimerai ir dnr kopolimero dangos
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
ES2768762T3 (es) 2014-11-05 2020-06-23 Illumina Cambridge Ltd Reducción del daño al ADN durante la preparación de muestras y secuenciación usando agentes quelantes sideróforos
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
CA2968543C (en) 2014-11-25 2024-04-02 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
MX2017008916A (es) 2015-01-13 2017-10-19 10X Genomics Inc Sistemas y metodos para visualizar informacion de fases y variaciones estructurales.
CN107208156B (zh) 2015-02-09 2021-10-08 10X基因组学有限公司 用于使用变异识别数据来确定结构变异和定相的***和方法
ES2824700T3 (es) 2015-02-19 2021-05-13 Becton Dickinson Co Análisis unicelular de alto rendimiento que combina información proteómica y genómica
CA2976580A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016147825A1 (ja) 2015-03-13 2016-09-22 シスメックス株式会社 被検物質の検出方法およびその方法に用いられる試薬キット
CA3225867A1 (en) 2015-03-24 2016-09-29 Illumina, Inc. Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
US11535882B2 (en) 2015-03-30 2022-12-27 Becton, Dickinson And Company Methods and compositions for combinatorial barcoding
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
DK3901281T3 (da) 2015-04-10 2023-01-23 Spatial Transcriptomics Ab Rumligt adskilt, multipleks nukleinsyreanalyse af biologiske prøver
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
WO2016183029A1 (en) 2015-05-11 2016-11-17 Illumina, Inc. Platform for discovery and analysis of therapeutic agents
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
JP6612539B2 (ja) * 2015-06-30 2019-11-27 シスメックス株式会社 遺伝子検査用検体処理装置
WO2017007753A1 (en) 2015-07-07 2017-01-12 Illumina, Inc. Selective surface patterning via nanoimrinting
US20180207920A1 (en) 2015-07-17 2018-07-26 Illumina, Inc. Polymer sheets for sequencing applications
IL305561A (en) 2015-07-30 2023-10-01 Illumina Inc Removal of orthogonal blocking of nucleotides
CN108474805A (zh) 2015-08-24 2018-08-31 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制***
US11302416B2 (en) 2015-09-02 2022-04-12 Guardant Health Machine learning for somatic single nucleotide variant detection in cell-free tumor nucleic acid sequencing applications
CN107921432A (zh) 2015-09-02 2018-04-17 伊卢米纳剑桥有限公司 改善流控***中的液滴操作的***和方法
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
US11123297B2 (en) 2015-10-13 2021-09-21 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
WO2017087206A1 (en) 2015-11-16 2017-05-26 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
JP2018535997A (ja) 2015-12-03 2018-12-06 ノバルティス アーゲー ctDNAにPIK3CA変異を有するために事前選択された患者におけるPI3K阻害剤を用いた癌の処置
EP3882357B1 (en) 2015-12-04 2022-08-10 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
CN108603228B (zh) 2015-12-17 2023-09-01 夸登特健康公司 通过分析无细胞dna确定肿瘤基因拷贝数的方法
TW201730563A (zh) 2016-01-11 2017-09-01 伊路米納有限公司 具有顯微螢光計、流體系統和流動單元閂鎖夾持模組的檢測裝置
JP6685138B2 (ja) 2016-01-27 2020-04-22 シスメックス株式会社 核酸増幅の精度管理方法、精度管理用試薬およびその試薬キット
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
JP2017143783A (ja) * 2016-02-17 2017-08-24 国立大学法人 筑波大学 並列反応用懸濁液、並列反応方法、スクリーニング方法および検査方法
US20170274374A1 (en) 2016-03-28 2017-09-28 Ilumina, Inc. Multi-plane microarrays
CN109072288A (zh) 2016-05-02 2018-12-21 赛卢拉研究公司 精确的分子条形编码
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10900076B2 (en) 2016-05-18 2021-01-26 Illumina, Inc. Self assembled patterning using patterned hydrophobic surfaces
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US11200963B2 (en) 2016-07-27 2021-12-14 Sequenom, Inc. Genetic copy number alteration classifications
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
EP3792922A1 (en) 2016-09-30 2021-03-17 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
RU2771563C2 (ru) 2016-10-14 2022-05-05 Иллюмина, Инк. Картриджный узел
CN109952612B (zh) 2016-11-08 2023-12-01 贝克顿迪金森公司 用于表达谱分类的方法
WO2018089377A1 (en) 2016-11-08 2018-05-17 Cellular Research, Inc. Methods for cell label classification
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
ES2961580T3 (es) 2017-01-13 2024-03-12 Cellular Res Inc Revestimiento hidrófilo de canales de fluidos
CA3050055C (en) 2017-01-24 2023-09-19 Sequenom, Inc. Methods and processes for assessment of genetic variations
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US20200087710A1 (en) 2017-03-17 2020-03-19 Sequenom, Inc. Methods and processes for assessment of genetic mosaicism
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
EP3635135A1 (en) 2017-06-05 2020-04-15 Becton, Dickinson and Company Sample indexing for single cells
WO2019035897A1 (en) 2017-08-15 2019-02-21 Omniome, Inc. SCANNING APPARATUS AND METHODS USEFUL FOR DETECTION OF CHEMICAL OR BIOLOGICAL ANALYTES
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
EP3697932A1 (en) 2017-10-19 2020-08-26 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN114525273A (zh) 2017-10-27 2022-05-24 10X基因组学有限公司 用于样品制备和分析的方法和***
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
EP3728636A1 (en) 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
CN112262218A (zh) 2018-04-06 2021-01-22 10X基因组学有限公司 用于单细胞处理中的质量控制的***和方法
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
EP4234718A3 (en) 2018-04-26 2023-11-29 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
CN108660191A (zh) * 2018-04-28 2018-10-16 中国科学院苏州生物医学工程技术研究所 一种基于编码微球反应器的数字化多重核酸检测方法
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
JP2021525078A (ja) 2018-05-31 2021-09-24 オムニオム インコーポレイテッドOmniome, Inc. 核酸配列決定におけるシグナル対ノイズの増加
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
EP3827097A1 (en) 2018-07-24 2021-06-02 Omniome, Inc. Serial formation of ternary complex species
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
WO2020101831A1 (en) * 2018-11-13 2020-05-22 Becton, Dickinson And Company Dried reagent strainers and methods for making and using the same
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
GB2585608B (en) 2018-12-04 2021-11-03 Omniome Inc Mixed-phase fluids for nucleic acid sequencing and other analytical assays
WO2020123319A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
MX2020013379A (es) 2018-12-19 2021-04-28 Illumina Inc Métodos para mejorar la clonalidad de grupos de polinucleótidos.
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322A3 (en) 2019-01-23 2023-09-20 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113661249A (zh) 2019-01-31 2021-11-16 夸登特健康公司 用于分离无细胞dna的组合物和方法
EP3924515A4 (en) * 2019-02-11 2022-08-24 Ultima Genomics, Inc. PROCEDURE FOR NUCLEIC ACID ANALYSIS
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
EP3924513B1 (en) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
WO2020172444A1 (en) 2019-02-20 2020-08-27 Omniome, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
EP3938537A1 (en) 2019-03-11 2022-01-19 10X Genomics, Inc. Systems and methods for processing optically tagged beads
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
CN114051534A (zh) 2019-07-22 2022-02-15 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4028407B1 (en) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible modification of nucleotides
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
CN118186063A (zh) 2019-10-18 2024-06-14 加利福尼亚太平洋生物科学股份有限公司 用于对核酸加帽的方法和组合物
WO2021087491A1 (en) 2019-10-31 2021-05-06 Sequenom, Inc. Application of mosaicism ratio in multifetal gestations and personalized risk assessment
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4219754B1 (en) 2019-12-23 2024-05-15 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
EP4100161A1 (en) 2020-02-04 2022-12-14 Pacific Biosciences of California, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
CN115836135A (zh) 2020-05-05 2023-03-21 加利福尼亚太平洋生物科学股份有限公司 用于修饰聚合酶-核酸复合物的组合物和方法
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CA3179883A1 (en) 2020-12-02 2022-06-09 Illumina Software, Inc. System and method for detection of genetic alterations
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4277740A1 (en) 2021-01-13 2023-11-22 Pacific Biosciences of California, Inc. Surface structuring with colloidal assembly
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4314327A1 (en) 2021-03-22 2024-02-07 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
EP4355476A1 (en) 2021-06-15 2024-04-24 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
KR20240031968A (ko) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 염기 호출에 대한 보간 및 적응을 갖는 강도 추출
EP4373958A1 (en) 2021-07-23 2024-05-29 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023049215A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Compressed state-based base calling
CA3234961A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
US20230348957A1 (en) 2021-12-07 2023-11-02 Luminex Corporation Methods and compositions for nucleic acid analysis
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
AU2023208743A1 (en) 2022-01-20 2024-01-04 Illumina, Inc. Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
WO2023183937A1 (en) 2022-03-25 2023-09-28 Illumina, Inc. Sequence-to-sequence base calling
US20230407386A1 (en) 2022-06-09 2023-12-21 Illumina, Inc. Dependence of base calling on flow cell tilt
WO2024072614A1 (en) 2022-09-27 2024-04-04 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US553851A (en) * 1896-02-04 Robert i-iardie
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US5395688A (en) * 1987-10-26 1995-03-07 Baxter Diagnostics Inc. Magnetically responsive fluorescent polymer particles
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
NO165894C (no) * 1988-05-24 1991-04-24 Gunnar Paulsen Analysemetode for gener.
FR2638848B1 (fr) * 1988-11-04 1993-01-22 Chemunex Sa Procede de detection et/ou de dosage dans un milieu liquide ou semi-liquide d'au moins une substance organique, biologique ou medicamenteuse soluble, par une methode d'agglutination
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
WO1991005058A1 (en) 1989-10-05 1991-04-18 Glenn Kawasaki Cell-free synthesis and isolation of novel genes and polypeptides
JPH06509473A (ja) 1991-08-10 1994-10-27 メディカル・リサーチ・カウンシル 細胞個体群の処理
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US20030105039A1 (en) * 1997-03-21 2003-06-05 Zarling David A. In vivo homologous sequence targeting in cells
DE4223169C1 (de) 1992-07-10 1993-11-25 Ferring Arzneimittel Gmbh Verfahren zur Mikroverkapselung wasserlöslicher Wirkstoffe
IL104384A (en) 1993-01-13 1996-11-14 Yeda Res & Dev Method for screening catalytic non-enzyme polypeptides and proteins
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
AU6815894A (en) 1993-04-19 1994-11-08 Stuart A. Kauffman Random chemistry for the generation of new compounds
NZ265818A (en) 1993-04-19 1997-09-22 Medisorb Technologies Internat Compositions of nucleic acids encapsulated with molecules that facilitate uptake and integration into living cells
AU8124694A (en) 1993-10-29 1995-05-22 Affymax Technologies N.V. In vitro peptide and antibody display libraries
GB9323305D0 (en) * 1993-11-11 1994-01-05 Medinnova Sf Isoaltion of nucleic acid
WO1995024929A2 (en) * 1994-03-15 1995-09-21 Brown University Research Foundation Polymeric gene delivery system
FR2726286B1 (fr) * 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
US5546792A (en) * 1994-12-22 1996-08-20 Harold L. Becker Computerized sonic portable testing laboratory
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
KR20040041639A (ko) 1995-04-24 2004-05-17 크로막솜 코포레이티드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
US5910408A (en) 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
DE69614768T2 (de) * 1995-06-14 2002-06-20 Tonen Corp Emulsionsspaltung durch Mikroorganismen
DE19646372C1 (de) 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US6022688A (en) 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
AU4596597A (en) 1996-09-27 1998-04-17 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
US6395524B2 (en) 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same
US6310354B1 (en) 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
EP1712623B1 (en) 1997-01-21 2011-10-19 The General Hospital Corporation Selection of proteins using RNA-protein fusions
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6023540A (en) * 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
CA2284066A1 (en) 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
DK1801214T3 (da) 1997-07-07 2011-01-24 Medical Res Council In vitro sorteringsfremgangsmåde
JP2981547B1 (ja) 1998-07-02 1999-11-22 農林水産省食品総合研究所長 クロスフロー型マイクロチャネル装置及び同装置を用いたエマルションの生成または分離方法
WO2000004139A1 (en) 1998-07-17 2000-01-27 Mirus Corporation Micellar systems
US6407210B1 (en) * 1998-11-09 2002-06-18 University Of Utah Research Foundation Nematode neuromuscular junction GABA receptors and methods related thereto
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
CA2370976C (en) 1999-04-20 2009-10-20 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
JP3911909B2 (ja) 1999-06-09 2007-05-09 株式会社日立製作所 Dna試料調製方法及びdna試料調製装置
US6461816B1 (en) * 1999-07-09 2002-10-08 Agilent Technologies, Inc. Methods for controlling cross-hybridization in analysis of nucleic acid sequences
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
GB9921155D0 (en) 1999-09-08 1999-11-10 Medical Res Council Selection system
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
AU2438501A (en) 1999-12-21 2001-07-03 Government Of The United States Of America, As Represented By The Secretary Of The Navy, The Expression of proteins from amplified, immobilized nucleic acids
US6403037B1 (en) * 2000-02-04 2002-06-11 Cepheid Reaction vessel and temperature control system
AU3838901A (en) * 2000-02-16 2001-08-27 Illumina Inc Parallel genotyping of multiple patient samples
CN1189159C (zh) * 2000-05-05 2005-02-16 欧莱雅 含水溶性美容活性组分水性核的微胶囊及含其的组合物
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
JP4741170B2 (ja) * 2000-10-11 2011-08-03 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー アニオン性リンカーを有する蛍光核酸塩基結合体
US6936264B2 (en) * 2001-03-05 2005-08-30 The Procter & Gamble Company Delivery of reactive agents via multiple emulsions for use in shelf stable products
GB0114854D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0114856D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selection by avidity capture
US20030064400A1 (en) * 2001-08-24 2003-04-03 Li-Cor, Inc. Microfluidics system for single molecule DNA sequencing
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
CA2469923A1 (en) * 2001-12-12 2003-06-19 Genset S.A. Biallelic markers of d-amino acid oxidase and uses thereof
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2003189862A (ja) * 2001-12-25 2003-07-08 Hitachi Software Eng Co Ltd ターゲット検出方法、dna塩基配列の一塩基多型検出用試薬及び一塩基多型検出方法
AUPS298102A0 (en) 2002-06-13 2002-07-04 Nucleics Pty Ltd Method for performing chemical reactions
CA2510166A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2513541A1 (en) * 2003-01-29 2004-08-19 454 Corporation Method for preparing single-stranded dna libraries
WO2005082098A2 (en) * 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US7604940B1 (en) * 2005-03-16 2009-10-20 Applied Biosystems, Llc Compositions and methods for analyzing isolated polynucleotides
AU2007261445B2 (en) 2006-06-19 2013-04-18 The Johns Hopkins University Single-molecule PCR on microparticles in water-in-oil emulsions

Also Published As

Publication number Publication date
EP2532745B1 (en) 2015-09-09
EP1641809A2 (en) 2006-04-05
US20160319341A1 (en) 2016-11-03
US9328343B2 (en) 2016-05-03
EP2532745A3 (en) 2014-03-12
EP1641809B2 (en) 2018-10-03
EP2918595A2 (en) 2015-09-16
JP2007527214A (ja) 2007-09-27
JP5183063B2 (ja) 2013-04-17
EP1641809A4 (en) 2010-05-19
US20070065823A1 (en) 2007-03-22
US10604797B2 (en) 2020-03-31
EP1641809B1 (en) 2015-09-09
WO2005010145A3 (en) 2005-08-11
CA2531105A1 (en) 2005-02-03
US8048627B2 (en) 2011-11-01
EP2918595A3 (en) 2015-10-21
EP2532745A2 (en) 2012-12-12
EP2918595B1 (en) 2019-12-11
US20090286687A1 (en) 2009-11-19
CA2531105C (en) 2015-03-17
WO2005010145A2 (en) 2005-02-03
US20120183967A1 (en) 2012-07-19

Similar Documents

Publication Publication Date Title
HK1179304A1 (en) Method and compositions for detection and enumeration of genetic variations
AU2003257013A1 (en) Methods and compositions for amplification of dna
GB0207944D0 (en) Method of detection
GB0514933D0 (en) Improved method of nucleotide detection
HK1085244A1 (en) Quantification of gene expression
HK1107098A1 (en) Methods and compositions for the inhibition of gene expression
EP1546397A4 (en) CELL-BASED RNA INTERFERENCE AND METHODS AND COMPOSITIONS RELATING THERETO
EP1633718A4 (en) COMPOSITIONS AND METHODS FOR INHIBITING TGF-S
AU2003239129A8 (en) Methods and compositions for dna manipulation
EP1831825A4 (en) SYSTEM AND METHOD FOR RECOGNIZING ABSOLUTE COORDINATES
GB0412654D0 (en) Method of detection
GB2431008B (en) System and method for enhanced measurement of rheological properties
EP1663255A4 (en) WITHANAMID AND WITHANOLID COMPOSITIONS AND APPLICATION METHOD THEREFOR
TWI318680B (en) Detection of macro-defects using micro-inspection inputs
IL182680A0 (en) Compositions and methods for short interfering nucleic acid inhibition of
EP1712563A4 (en) METHOD FOR ISOLATING MONOCYTES
AU2003295692A1 (en) Methods and compositions for analysis of regulatory sequences
EP1615942A4 (en) Polymerase inhibitor and method for its use
IL158097A (en) Method and system for detection of objects
EP1514946A4 (en) METHOD FOR DETECTING GENE POLYMORPHISM
GB0303936D0 (en) Method of testing
AU2003263271A8 (en) Method capable of being automated for detection of prpless thanspgreater thanresless than/spgreater than and uses thereof
AU2003219719A8 (en) Methods and compositions for detecting differences between nucleic acids
EP1672071A4 (en) MUTATION DETECTION METHOD AND METHOD FOR INDUCING SUCH MUTATION
FI20020651A0 (fi) Geenikartoitusmenetelmä geno- ja fenotyyppikatasta