SG10201911069WA - High-throughput nucleotide library sequencing - Google Patents

High-throughput nucleotide library sequencing

Info

Publication number
SG10201911069WA
SG10201911069WA SG10201911069WA SG10201911069WA SG10201911069WA SG 10201911069W A SG10201911069W A SG 10201911069WA SG 10201911069W A SG10201911069W A SG 10201911069WA SG 10201911069W A SG10201911069W A SG 10201911069WA SG 10201911069W A SG10201911069W A SG 10201911069WA
Authority
SG
Singapore
Prior art keywords
library sequencing
nucleotide library
throughput
throughput nucleotide
sequencing
Prior art date
Application number
SG10201911069WA
Inventor
Francois Vigneault
Briggs Adrian Wrangham
Christopher Ryan Clouser
Stephen Jacob Goldfless
Sonia Timberlake
Original Assignee
Abvitro Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abvitro Llc filed Critical Abvitro Llc
Publication of SG10201911069WA publication Critical patent/SG10201911069WA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/179Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/514Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Chemical Kinetics & Catalysis (AREA)
SG10201911069WA 2014-09-15 2015-09-15 High-throughput nucleotide library sequencing SG10201911069WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462050549P 2014-09-15 2014-09-15
US201462051832P 2014-09-17 2014-09-17

Publications (1)

Publication Number Publication Date
SG10201911069WA true SG10201911069WA (en) 2020-01-30

Family

ID=54291588

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201911069WA SG10201911069WA (en) 2014-09-15 2015-09-15 High-throughput nucleotide library sequencing
SG11201702060VA SG11201702060VA (en) 2014-09-15 2015-09-15 High-throughput nucleotide library sequencing

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201702060VA SG11201702060VA (en) 2014-09-15 2015-09-15 High-throughput nucleotide library sequencing

Country Status (13)

Country Link
US (3) US10590483B2 (en)
EP (3) EP3950944A1 (en)
JP (3) JP6672310B2 (en)
KR (1) KR102541849B1 (en)
CN (1) CN107002076B (en)
AU (2) AU2015318011B2 (en)
CA (1) CA2961210A1 (en)
ES (2) ES2895750T3 (en)
IL (1) IL251123B (en)
MX (1) MX2017003382A (en)
SG (2) SG10201911069WA (en)
WO (1) WO2016044227A1 (en)
ZA (1) ZA201701916B (en)

Families Citing this family (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CN104364392B (en) 2012-02-27 2018-05-25 赛卢拉研究公司 For the composition and kit of numerator counts
IN2015DN01126A (en) 2012-08-14 2015-06-26 10X Genomics Inc
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
DK2954065T3 (en) 2013-02-08 2021-09-06 10X Genomics Inc Division and processing of analytes and other species
GB2584364A (en) 2013-03-15 2020-12-02 Abvitro Llc Single cell bar-coding for antibody discovery
KR20230074639A (en) 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
CN114534806B (en) 2014-04-10 2024-03-29 10X基因组学有限公司 Fluidic devices, systems and methods for packaging and partitioning reagents and uses thereof
WO2015188839A2 (en) 2014-06-13 2015-12-17 Immudex Aps General detection and isolation of specific cells by binding of labeled molecules
KR20230070325A (en) 2014-06-26 2023-05-22 10엑스 제노믹스, 인크. Methods of analyzing nucleic acids from individual cells or cell populations
MX2017003382A (en) 2014-09-15 2017-11-20 Abvitro Llc High-throughput nucleotide library sequencing.
CN107002128A (en) 2014-10-29 2017-08-01 10X 基因组学有限公司 The method and composition being sequenced for target nucleic acid
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
DE102014017085A1 (en) 2014-11-20 2016-05-25 Kautex Textron Gmbh & Co. Kg Device for producing a reinforcing structure on a shaped body surface
BR112017014902A2 (en) 2015-01-12 2018-03-13 10X Genomics Inc processes and systems for the preparation of nucleic acid sequencing libraries and libraries prepared using them
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
ES2836802T3 (en) 2015-02-27 2021-06-28 Becton Dickinson Co Spatially addressable molecular barcodes
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
US10788452B2 (en) * 2015-04-21 2020-09-29 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
AU2016250591B2 (en) 2015-04-21 2021-08-05 Isolation Bio Inc. High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
JP6894380B2 (en) 2015-04-27 2021-06-30 アブビトロ, エルエルシー Methods for Sequencing, Determining, Pairing and Verifying Therapeutic Drugs and Disease-Specific Antigens
ES2745694T3 (en) 2015-09-11 2020-03-03 Cellular Res Inc Methods and compositions for nucleic acid library normalization
CN108289954B (en) 2015-09-24 2022-05-31 阿布维特罗有限责任公司 HIV antibody compositions and methods of use
BR112018005937A2 (en) 2015-09-24 2019-05-21 Abvitro Llc affinity oligonucleotide conjugates and uses of these
KR20180097536A (en) * 2015-11-04 2018-08-31 아트레카, 인크. A combination set of nucleic acid barcodes for the analysis of nucleic acids associated with single cells
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
EP4299136A3 (en) 2015-12-16 2024-02-14 Gritstone bio, Inc. Neoantigen identification, manufacture, and use
US11117113B2 (en) 2015-12-16 2021-09-14 Fluidigm Corporation High-level multiplex amplification
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
SG11201811048UA (en) * 2016-07-14 2019-01-30 Fluidigm Corp Single-cell transcript sequencing
US10683531B2 (en) 2016-09-15 2020-06-16 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free DNA
CN109937254B (en) 2016-09-15 2023-05-30 阿谢尔德克斯有限责任公司 Nucleic acid sample preparation method
JP6929354B2 (en) * 2016-09-24 2021-09-01 アブビトロ, エルエルシー Affinity-oligonucleotide conjugates and their use
JP7091348B2 (en) 2016-09-26 2022-06-27 ベクトン・ディキンソン・アンド・カンパニー Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11072660B2 (en) 2016-10-03 2021-07-27 Juno Therapeutics, Inc. HPV-specific binding molecules
WO2018075693A1 (en) * 2016-10-19 2018-04-26 10X Genomics, Inc. Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations
CA3042391A1 (en) 2016-11-02 2018-05-11 ArcherDX, Inc. Methods of nucleic acid sample preparation for immune repertoire sequencing
US20190316180A1 (en) * 2016-11-23 2019-10-17 Universite De Strasbourg Tandem barcoding of target molecules for their absolute quantification at single-entity resolution
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
MY201744A (en) 2016-12-23 2024-03-15 Visterra Inc Binding polypeptides and methods of making the same
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
CN110199022A (en) * 2017-02-16 2019-09-03 宝生物工程(美国) 有限公司 Prepare the method for nucleic acid library and composition and kit for implementing the method
WO2018208133A1 (en) * 2017-05-12 2018-11-15 서울대학교산학협력단 Method and apparatus for acquiring high-purity nucleotides
CN116064732A (en) 2017-05-26 2023-05-05 10X基因组学有限公司 Single cell analysis of transposase accessibility chromatin
AU2018273999A1 (en) 2017-05-26 2019-12-05 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2019010486A1 (en) * 2017-07-07 2019-01-10 Board Of Regents, The University Of Texas System High-coverage and ultra-accurate immune repertoire sequencing using molecular identifiers
MX2020001491A (en) 2017-08-09 2020-08-06 Juno Therapeutics Inc Methods and compositions for preparing genetically engineered cells.
EP3679370A1 (en) 2017-09-07 2020-07-15 Juno Therapeutics, Inc. Methods of identifying cellular attributes related to outcomes associated with cell therapy
JP2020537515A (en) 2017-10-03 2020-12-24 ジュノー セラピューティクス インコーポレイテッド HPV-specific binding molecule
AU2018348165A1 (en) 2017-10-10 2020-05-21 Gritstone Bio, Inc. Neoantigen identification using hotspots
CN111051523B (en) 2017-11-15 2024-03-19 10X基因组学有限公司 Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3714275A4 (en) 2017-11-22 2021-10-27 Gritstone bio, Inc. Reducing junction epitope presentation for neoantigens
EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
CN111699042A (en) 2017-12-07 2020-09-22 麻省理工学院 Single cell analysis
JP2021506260A (en) 2017-12-15 2021-02-22 ジュノー セラピューティクス インコーポレイテッド Anti-CCT5 binding molecule and how to use it
EP3728636A1 (en) * 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
CN108866174B (en) * 2017-12-25 2023-05-19 厦门基源医疗科技有限公司 Method for detecting low-frequency mutation of circulating tumor DNA
CN108085341A (en) * 2017-12-28 2018-05-29 河南省华隆生物技术有限公司 A kind of MSGV recombinant vectors and its preparation method and application
CN112236518A (en) * 2018-02-23 2021-01-15 耶鲁大学 Single cell freeze-thaw lysis
CN112566698A (en) 2018-04-05 2021-03-26 朱诺治疗学股份有限公司 T cell receptor and engineered cells expressing the same
CN112262218A (en) 2018-04-06 2021-01-22 10X基因组学有限公司 System and method for quality control in single cell processing
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
CN109251961A (en) * 2018-06-28 2019-01-22 广西医科大学 A kind of method of unicellular sequencing detection activating T cell
CN112673100A (en) * 2018-07-09 2021-04-16 格罗生物科学公司 Compositions comprising non-standard amino acids and uses thereof
KR102543325B1 (en) * 2018-08-13 2023-06-13 루트패스 제노믹스, 인크. High-throughput cloning of paired binary immunoreceptor polynucleotides and their applications
WO2020046833A1 (en) * 2018-08-28 2020-03-05 Cellular Research, Inc. Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
WO2020072380A1 (en) * 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
PE20212198A1 (en) 2019-01-29 2021-11-16 Juno Therapeutics Inc ANTIBODIES AND CHIMERIC RECEPTORS OF SPECIFIC ANTIGENS TO ORPHAN RECEPTOR 1, RECEPTOR TYROSINE KINASE TYPE (ROR1)
WO2020206238A2 (en) 2019-04-05 2020-10-08 Rootpath Genomics, Inc. Compositions and methods for t-cell receptor gene assembly
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN110241460A (en) * 2019-05-31 2019-09-17 南方医科大学南方医院 A kind of immune group library method for screening the reaction of independent sample crossover
CN110241459A (en) * 2019-05-31 2019-09-17 南方医科大学南方医院 A kind of immune group library method screening sample room and being reacted with independent sample crossover
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
WO2021023853A1 (en) * 2019-08-08 2021-02-11 INSERM (Institut National de la Santé et de la Recherche Médicale) Rna sequencing method for the analysis of b and t cell transcriptome in phenotypically defined b and t cell subsets
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
KR20230024283A (en) 2020-05-13 2023-02-20 주노 쎄러퓨티크스 인코퍼레이티드 Methods for identifying features associated with clinical response and uses thereof
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022081643A2 (en) * 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
CN112071366B (en) * 2020-10-13 2024-02-27 南开大学 Metagenome data analysis method based on second-generation sequencing technology
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN114958996B (en) * 2021-05-12 2022-12-20 浙江大学 Ultrahigh-throughput unicellular sequencing reagent combination
EP4355914A1 (en) * 2021-06-18 2024-04-24 Adoc Ssf, Llc Methods for improved t cell receptor sequencing
CN115029341A (en) * 2022-05-23 2022-09-09 立凌生物制药(苏州)有限公司 Rapid clone pairing TCR sequence detection method and application thereof
WO2024015862A1 (en) * 2022-07-13 2024-01-18 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples

Family Cites Families (194)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4766067A (en) 1985-05-31 1988-08-23 President And Fellows Of Harvard College Gene amplification
GB8601597D0 (en) 1986-01-23 1986-02-26 Wilson R H Nucleotide sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4795699A (en) 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US4921794A (en) 1987-01-14 1990-05-01 President And Fellows Of Harvard College T7 DNA polymerase
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5168038A (en) 1988-06-17 1992-12-01 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5142033A (en) 1988-09-23 1992-08-25 Hoffmann-La Roche Inc. Structure-independent DNA amplification by the polymerase chain reaction
US4994370A (en) 1989-01-03 1991-02-19 The United States Of America As Represented By The Department Of Health And Human Services DNA amplification technique
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
DE3920358A1 (en) 1989-06-22 1991-01-17 Behringwerke Ag BISPECIFIC AND OLIGO-SPECIFIC, MONO- AND OLIGOVALENT ANTI-BODY CONSTRUCTS, THEIR PRODUCTION AND USE
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
AU660306B2 (en) 1991-02-12 1995-06-22 Roussel-Uclaf Nucleotidic sequences coding for variable regions of beta chains of human T lymphocyte receptors, corresponding peptidic segments and diagnostic and therapeutic applications
CA2124087C (en) 1991-11-22 2002-10-01 James L. Winkler Combinatorial strategies for polymer synthesis
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
US5550215A (en) 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
CA2122732C (en) 1991-11-25 2008-04-08 Marc D. Whitlow Multivalent antigen-binding proteins
EP0746609A4 (en) 1991-12-17 1997-12-17 Genpharm Int Transgenic non-human animals capable of producing heterologous antibodies
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
EP0705271B1 (en) 1993-06-25 2002-11-13 Affymetrix, Inc. (a Delaware Corporation) Hybridization and sequencing of nucleic acids
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US6090555A (en) 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
WO1995030774A1 (en) 1994-05-05 1995-11-16 Beckman Instruments, Inc. Oligonucleotide repeat arrays
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
EP0902885A4 (en) 1996-05-16 2006-09-27 Affymetrix Inc Systems and methods for detection of labeled materials
WO1997045559A1 (en) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
PT1179600E (en) 1996-06-04 2005-08-31 Univ Utah Res Found HIBRIDATION MONITORING DURING CPR
PT937251E (en) 1996-09-06 2007-01-31 Ortho Mcneil Pharm Inc Purification of antigen-specific t cells
ATE545710T1 (en) 1997-04-01 2012-03-15 Illumina Cambridge Ltd METHOD FOR THE DUPLICATION OF NUCLEIC ACIDS
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
WO1999023254A1 (en) 1997-10-31 1999-05-14 Affymetrix, Inc. Expression profiles in adult and fetal organs
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US6428752B1 (en) 1998-05-14 2002-08-06 Affymetrix, Inc. Cleaning deposit devices that form microarrays and the like
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US5936324A (en) 1998-03-30 1999-08-10 Genetic Microsystems Inc. Moving magnet scanner
DE69933369D1 (en) 1998-04-03 2006-11-09 Compound Therapeutics Inc Adressierbare protein arrays
JP3813818B2 (en) 1998-05-01 2006-08-23 アリゾナ ボード オブ リージェンツ Method for determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
CA2366459A1 (en) 1999-03-26 2000-10-05 Affymetrix, Inc. Universal arrays
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
GB9928787D0 (en) 1999-12-03 2000-02-02 Medical Res Council Direct screening method
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
JP5508654B2 (en) 2000-04-14 2014-06-04 コーネル・リサーチ・ファンデーション・インコーポレイテッド Method for designing positionable arrays for detection of nucleic acid sequence differences using ligase detection reactions
US6686184B1 (en) 2000-05-25 2004-02-03 President And Fellows Of Harvard College Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks
US7567870B1 (en) 2000-07-31 2009-07-28 Institute For Systems Biology Multiparameter analysis for predictive medicine
AU1092602A (en) 2000-10-24 2002-05-06 New Ind Res Organization Genomes participating in rheumatoid arthritis, method of the diagnosis thereof, method of evaluating the onset possibility thereof, diagnostic kit for detectingthem and therapeutic method and remedies for rheumatoid arthritis
US7393656B2 (en) 2001-07-10 2008-07-01 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for risk stratification
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
AU2003247129A1 (en) 2002-07-01 2004-01-19 Centre National De La Recherche Scientifique (Cnrs) System, method, device, and computer program product for extraction, gathering, manipulation, and analysis of peak data from an automated sequencer
EP1517995A4 (en) 2002-07-03 2007-07-11 Inst Scient Res Inc Compositions and methods for the detection of human t cell receptor variable family gene expression
AU2003283976B2 (en) 2002-09-27 2009-12-10 Cold Spring Harbor Laboratory Cell-based RNA interference and related methods and compositions
DK1549764T3 (en) 2002-10-11 2011-01-24 Univ Erasmus Primers for nucleic acid amplification in PCR-based clonality studies
EP2159285B1 (en) 2003-01-29 2012-09-26 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
AU2004229440B2 (en) 2003-04-10 2010-08-12 President And Fellows Of Harvard College Formation and control of fluidic species
AU2003902299A0 (en) 2003-05-13 2003-05-29 Flinders Medical Centre A method of analysing a marker nucleic acid molecule
WO2005010145A2 (en) 2003-07-05 2005-02-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
US7767435B2 (en) 2003-08-25 2010-08-03 University Of Washington Method and device for biochemical detection and analysis of subcellular compartments from a single cell
JP4630870B2 (en) 2003-08-27 2011-02-09 プレジデント アンド フェロウズ オブ ハーバード カレッジ Electronic control of fluid species
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
FR2863274B1 (en) 2003-12-05 2012-11-16 Commissariat Energie Atomique METHOD FOR QUANTITATIVE EVALUATION OF REARRANGEMENT OR TARGETED GENETIC RECOMBINATION OF AN INDIVIDUAL AND APPLICATIONS THEREOF
EP1704251A1 (en) 2003-12-15 2006-09-27 Institut Pasteur Repertoire determination of a lymphocyte b population
US20080166718A1 (en) 2003-12-15 2008-07-10 Institut Pasteur Repertoire determination of a lymphocyte B population
US20060046258A1 (en) 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
WO2005084134A2 (en) * 2004-03-04 2005-09-15 Dena Leshkowitz Quantifying and profiling antibody and t cell receptor gene expression
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US7820382B2 (en) 2004-08-03 2010-10-26 Bauer A Robert Method for the early detection of breast cancer, lung cancer, pancreatic cancer and colon polyps, growths and cancers as well as other gastrointestinal disease conditions and the preoperative and postoperative monitoring of transplanted organs from the donor and in the recipient and their associated conditions related and unrelated to the organ transplantation
US20080280282A1 (en) 2004-08-03 2008-11-13 Bauer Jr A Robert Method for early detection of various cancers and gastrointestinal disease and monitoring of transplanted organs
US20060094018A1 (en) 2004-08-03 2006-05-04 Bauer A R Jr Discovery and a method for the early detection of pancreatic cancer and other disease conditions
WO2006031221A1 (en) 2004-09-13 2006-03-23 Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Compositions comprising t cell receptors and methods of use thereof
US7643818B2 (en) 2004-11-22 2010-01-05 Seven Networks, Inc. E-mail messaging to/from a mobile terminal
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
EP2485052B1 (en) 2005-09-13 2015-05-06 Affymetrix, Inc. Encoded microparticles
US9360526B2 (en) 2005-10-24 2016-06-07 The Johns Hopkins University Methods for beaming
US7375211B2 (en) 2005-11-18 2008-05-20 Kou Zhong C Method for detection and quantification of T-cell receptor Vβ repertoire
US20070161031A1 (en) 2005-12-16 2007-07-12 The Board Of Trustees Of The Leland Stanford Junior University Functional arrays for high throughput characterization of gene expression regulatory elements
WO2007081387A1 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices, methods of use, and kits for performing diagnostics
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
CA2640024A1 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
MX2008011280A (en) 2006-03-06 2008-09-12 Symphogen As Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections.
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
CA2656022C (en) 2006-06-19 2017-10-17 The Johns Hopkins University Single-molecule pcr on microparticles in water-in-oil emulsions
US20080108804A1 (en) 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
WO2008066596A2 (en) 2006-11-30 2008-06-05 Johns Hopkins University Gene expression barcode for normal and diseased tissue classification
WO2008076842A2 (en) 2006-12-14 2008-06-26 Applied Biosystems Inc. Sequencing methods
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
EP2173509A4 (en) 2007-06-25 2013-11-06 Affymetrix Inc Patterned microcodes
CA2691940C (en) 2007-07-03 2018-03-06 Joost Alexander Kolkman Methods for providing improved immunoglobulin sequences
EP2036989B1 (en) 2007-09-12 2012-07-25 Institut Pasteur Polynucleotide suitable for single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
WO2009045898A2 (en) 2007-09-28 2009-04-09 Mayo Foundation For Medical Education And Research Assessing t cell repertoires
EP2062982A1 (en) 2007-11-26 2009-05-27 ImmunID Method for studying the V(D)J combinatorial diversity.
US20110008775A1 (en) 2007-12-10 2011-01-13 Xiaolian Gao Sequencing of nucleic acids
US20090163366A1 (en) 2007-12-24 2009-06-25 Helicos Biosciences Corporation Two-primer sequencing for high-throughput expression analysis
EP2100970B1 (en) 2008-03-13 2017-05-10 National Institute of Immunology Ig genes specific oligonucleotides and uses thereof
US9012148B2 (en) 2008-04-16 2015-04-21 Jian Han Method for evaluating and comparing immunorepertoires
EP2644709B1 (en) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US20100062494A1 (en) 2008-08-08 2010-03-11 President And Fellows Of Harvard College Enzymatic oligonucleotide pre-adenylation
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
AU2009297108B2 (en) 2008-09-23 2015-02-12 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9115399B2 (en) 2008-09-23 2015-08-25 The Trustees Of The University Of Pennsylvania Recombination sequence (RS) rearrangement frequency as a measure of central B cell tolerance
CN102227440A (en) 2008-09-30 2011-10-26 雅培制药有限公司 Improved antibody libraries
EP2719774B8 (en) 2008-11-07 2020-04-22 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US20140037645A1 (en) 2008-11-07 2014-02-06 National Jewish Health Diagonsis and treatment of autoimmune diseases by targeting autoimmune-related b cells ("abcs")
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
DK3059337T3 (en) 2009-01-15 2019-07-22 Adaptive Biotechnologies Corp Adaptive immunity profiling and methods for producing monoclonal antibodies
AU2010206843B2 (en) 2009-01-20 2015-01-29 The Board Of Trustees Of The Leland Stanford Junior University Single cell gene expression for diagnosis, prognosis and identification of drug targets
US9079942B2 (en) 2009-02-09 2015-07-14 Epitomics, Inc. CDR-anchored amplification method
GB0905023D0 (en) 2009-03-24 2009-05-06 Univ Erasmus Medical Ct Binding molecules
ES2903425T3 (en) * 2009-03-30 2022-04-01 Illumina Inc Gene expression analysis in single cells
WO2010138621A2 (en) 2009-05-26 2010-12-02 The Uab Research Foundation Diagnosing and treating iga nephropathy
KR20120060948A (en) 2009-05-29 2012-06-12 더 리전트 오브 더 유니버시티 오브 캘리포니아 B cell signature associated with tolerance in transplant recipients
SG10201403451QA (en) 2009-06-25 2014-09-26 Hutchinson Fred Cancer Res Method of measuring adaptive immunity
US8497071B2 (en) 2009-06-29 2013-07-30 California Institute Of Technology Isolation of unknown rearranged T-cell receptors from single cells
GB2471473A (en) 2009-06-30 2011-01-05 Owen Mumford Ltd Syringe sheath remover
WO2011025826A1 (en) 2009-08-26 2011-03-03 Research Development Foundation Methods for creating antibody libraries
US8293483B2 (en) 2009-09-11 2012-10-23 Epitomics, Inc. Method for identifying lineage-related antibodies
US20110086051A1 (en) 2009-10-08 2011-04-14 Dartmouth-Hitchcock Clinic System and method for monitoring and optimizing immune status in transplant recipients
WO2011049603A1 (en) 2009-10-22 2011-04-28 Dana-Farber Cancer Institute, Inc. Biomarkers to identify hiv-specific t-cell subsets
GB0918564D0 (en) 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
ES2821500T3 (en) 2009-11-06 2021-04-26 Univ Leland Stanford Junior Non-invasive diagnosis of graft rejection in organ transplant patients
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
EP2539470B1 (en) 2010-02-24 2017-02-22 The Board of Trustees of The Leland Stanford Junior University Methods for autoimmune disease diagnosis, prognosis, and treatment
CA2794255C (en) 2010-03-25 2020-01-14 Minnie M. Sarwal Protein and gene biomarkers for rejection of organ transplants
EP3456847A1 (en) 2010-05-06 2019-03-20 Adaptive Biotechnologies Corporation Monitoring solid transplant rejection using clonotype profiles
US9234240B2 (en) 2010-05-07 2016-01-12 The Board Of Trustees Of The Leland Stanford Junior University Measurement and comparison of immune diversity by high-throughput sequencing
CA2799746C (en) 2010-05-17 2020-11-24 Sai Reddy Rapid isolation of monoclonal antibodies from animals
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
WO2012027503A2 (en) 2010-08-24 2012-03-01 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
JP5992911B2 (en) 2010-09-21 2016-09-14 ポピュレーション ジェネティクス テクノロジーズ リミテッド Increasing the reliability of allelic calls by molecular counting
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
US9902950B2 (en) * 2010-10-08 2018-02-27 President And Fellows Of Harvard College High-throughput single cell barcoding
DK2625295T3 (en) 2010-10-08 2019-06-11 Harvard College HIGH-THROUGHPUT-IMMUNE SEQUENCING
WO2012072705A1 (en) 2010-12-01 2012-06-07 Morphosys Ag Simultaneous detection of biomolecules in single cells
US9193997B2 (en) 2010-12-15 2015-11-24 The Board Of Trustees Of The Leland Stanford Junior University Measuring and monitoring of cell clonality
EP2652155B1 (en) 2010-12-16 2016-11-16 Gigagen, Inc. Methods for massively parallel analysis of nucleic acids in single cells
US10670608B2 (en) 2010-12-31 2020-06-02 Bioatla, Llc Comprehensive monoclonal antibody generation
DK2663864T3 (en) 2011-01-14 2019-06-24 Irepertoire Inc PROCEDURE TO EVALUATE IMMEDIATE VARIETY AND APPLICATION OF THIS
US9150852B2 (en) * 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
ES2625288T3 (en) 2011-04-15 2017-07-19 The Johns Hopkins University Secure Sequencing System
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
EP3415619B1 (en) * 2011-04-28 2020-12-16 The Board of Trustees of the Leland Stanford Junior University Identification of polynucleotides associated with a sample
EP3216872B1 (en) 2011-06-02 2020-04-01 Bio-Rad Laboratories, Inc. Enzyme quantification
EP3034513B1 (en) 2011-07-12 2020-09-02 XBiotech, Inc Identifying affinity-matured human antibodies
GB2496016B (en) 2011-09-09 2016-03-16 Univ Leland Stanford Junior Methods for obtaining a sequence
US20130078633A1 (en) 2011-09-22 2013-03-28 ImmuMetrix, LLC Detection of Isotype Profiles as Signatures for Disease
US9176031B2 (en) * 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
EP2823064B1 (en) * 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
CN104903466B (en) 2012-11-05 2016-11-23 鲁比康基因组学公司 Bar coding nucleic acid
GB2584364A (en) 2013-03-15 2020-12-02 Abvitro Llc Single cell bar-coding for antibody discovery
US20160122753A1 (en) * 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
PL3030682T3 (en) * 2013-08-05 2020-11-16 Twist Bioscience Corporation De novo synthesized gene libraries
WO2015103339A1 (en) * 2013-12-30 2015-07-09 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
ES2819277T3 (en) 2014-02-11 2021-04-15 Hoffmann La Roche Directed Sequencing and UID Filtering
AU2015222944A1 (en) * 2014-02-27 2016-09-08 Massachusetts Institute Of Technology T cell balance gene expression, compositions of matters and methods of use thereof
MX2017003382A (en) 2014-09-15 2017-11-20 Abvitro Llc High-throughput nucleotide library sequencing.

Also Published As

Publication number Publication date
EP3536786B1 (en) 2021-06-30
CA2961210A1 (en) 2016-03-24
AU2015318011A1 (en) 2017-04-06
EP3950944A1 (en) 2022-02-09
JP6672310B2 (en) 2020-03-25
US20240076732A1 (en) 2024-03-07
JP2017527313A (en) 2017-09-21
CN107002076A (en) 2017-08-01
SG11201702060VA (en) 2017-04-27
EP3536786A1 (en) 2019-09-11
IL251123A0 (en) 2017-04-30
IL251123B (en) 2020-08-31
US10590483B2 (en) 2020-03-17
EP3194593A1 (en) 2017-07-26
AU2020204449B2 (en) 2022-03-17
JP6960490B2 (en) 2021-11-05
AU2020204449A1 (en) 2020-07-23
US20200239955A1 (en) 2020-07-30
ZA201701916B (en) 2020-10-28
ES2727656T3 (en) 2019-10-17
US20160244825A1 (en) 2016-08-25
MX2017003382A (en) 2017-11-20
KR20170063726A (en) 2017-06-08
EP3194593B1 (en) 2019-02-06
KR102541849B1 (en) 2023-06-09
AU2015318011B2 (en) 2020-07-23
ES2895750T3 (en) 2022-02-22
JP2022023115A (en) 2022-02-07
WO2016044227A1 (en) 2016-03-24
JP2020108382A (en) 2020-07-16
CN107002076B (en) 2021-11-23
JP7278352B2 (en) 2023-05-19

Similar Documents

Publication Publication Date Title
ZA201701916B (en) High-throughput nucleotide library sequencing
HK1256688A1 (en) Nuclease-mediated dna assembly
HK1245830A1 (en) Sequencing controls
SG11201706636PA (en) Single-cell nucleic acids for high-throughput studies
EP3174993C0 (en) Probe library construction
EP3394292A4 (en) Methods of library construction for polynucleotide sequencing
SG11201610168YA (en) Nucleic acid synthesis techniques
IL250836A0 (en) Nucleotide analogs
HK1243958A1 (en) Nucleic acid construct
GB201507119D0 (en) Nucleic Acid Construct
GB201412977D0 (en) Single nucleotide detection method
HK1245346A1 (en) High-throughput sequencing of polynucleotides
GB201507115D0 (en) Nucleic Acid Construct
GB201507111D0 (en) Nucleic acid construct
GB201507104D0 (en) Nucleic acid construct
SG11201609754QA (en) Sequencing process
GB201418144D0 (en) High throughput sequencing
IL257500A (en) Modified cullin1 gene
GB201517241D0 (en) DNA modification
GB201415674D0 (en) Nucleic acid analysis
GB201402996D0 (en) Variant analysis in high-throughput sequencing applications
GB201709308D0 (en) Variant nucleotide library
GB201503465D0 (en) Sequencing methods
GB201416211D0 (en) Sequencing techniques
GB201409785D0 (en) Sequencing Techniques