ES457925A1 - Un procedimiento para la preparacion de una enzima alfa-ami-lasa estable al calor y a los acidos. - Google Patents

Un procedimiento para la preparacion de una enzima alfa-ami-lasa estable al calor y a los acidos.

Info

Publication number
ES457925A1
ES457925A1 ES457925A ES457925A ES457925A1 ES 457925 A1 ES457925 A1 ES 457925A1 ES 457925 A ES457925 A ES 457925A ES 457925 A ES457925 A ES 457925A ES 457925 A1 ES457925 A1 ES 457925A1
Authority
ES
Spain
Prior art keywords
minutes
calcium ions
alpha
amylase activity
retain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES457925A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever Bestfoods North America
Original Assignee
Unilever Bestfoods North America
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Bestfoods North America filed Critical Unilever Bestfoods North America
Publication of ES457925A1 publication Critical patent/ES457925A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)

Abstract

Una enzima alfa-amilasa estable al calor y a los ácidos caracterizado porque (1) es capaz de retener por lo menos aproximadamente 70% de su actividad inicial de alfa-amilasa cuando se mantiene a 90ºC y a un pH de 6,0 durante 10 minutos en ausencia de iones calcio añadidos; (2) es capaz de retener por lo menos aproximadamente 50% de su actividad inicial de alfa-amilasa cuando se mantiene a 90ºC a un pH de 6,0 durante 60 minutos en ausencia de iones calcio añadidos; y (3) es capaz de retener por lo menos aproximadamente 50% de retener por lo menos aproximadamente 50% de su actividad inicial de alfa-amilasa a una temperatura de 80ºC y a un pH de 4,55 en presencia de 5mM de iones calcio durante 10 minutos.
ES457925A 1976-04-19 1977-04-18 Un procedimiento para la preparacion de una enzima alfa-ami-lasa estable al calor y a los acidos. Expired ES457925A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67851376A 1976-04-19 1976-04-19
US76492377A 1977-02-02 1977-02-02

Publications (1)

Publication Number Publication Date
ES457925A1 true ES457925A1 (es) 1978-03-01

Family

ID=27102035

Family Applications (1)

Application Number Title Priority Date Filing Date
ES457925A Expired ES457925A1 (es) 1976-04-19 1977-04-18 Un procedimiento para la preparacion de una enzima alfa-ami-lasa estable al calor y a los acidos.

Country Status (16)

Country Link
JP (1) JPS52151790A (es)
AR (1) AR218023A1 (es)
AU (1) AU513863B2 (es)
BR (1) BR7702475A (es)
CA (1) CA1081633A (es)
DE (1) DE2717333C2 (es)
DK (1) DK149157C (es)
ES (1) ES457925A1 (es)
FI (1) FI58157C (es)
FR (1) FR2348930A1 (es)
GB (1) GB1539694A (es)
IT (1) IT1080353B (es)
MY (1) MY8000095A (es)
NL (1) NL7704280A (es)
NZ (1) NZ183818A (es)
YU (1) YU40476B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1170202A (en) * 1981-01-15 1984-07-03 Susan Mickel Process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis
US4376824A (en) * 1981-04-27 1983-03-15 Nabisco Brands, Inc. Process for producing glucose/fructose syrups from unrefined starch hydrolysates
GB2129806B (en) * 1982-10-29 1986-04-03 Cpc International Inc Process for preparing high-dextrose starch hydrolysates with immobilized glucoamylase
GB8414272D0 (en) * 1984-06-05 1984-07-11 Cpc International Inc Enzymatic hydrolysis
US5010008A (en) * 1988-12-12 1991-04-23 Enzyme Bio-Systems Ltd. Stable liquid enzyme concentrate and process for its production
JP2019094420A (ja) * 2017-11-22 2019-06-20 株式会社ニイタカ 洗浄剤組成物、浸漬用洗浄剤及び食器洗浄機用洗浄剤
CN110423737B (zh) * 2019-09-10 2021-04-30 白银赛诺生物科技有限公司 来源于嗜热脂肪土芽孢杆菌的耐热型α-淀粉酶及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2695863A (en) * 1952-04-16 1954-11-30 Purdue Research Foundation Process for preparing alpha amylase
GB1285173A (en) * 1968-09-09 1972-08-09 Glaxo Lab Ltd AN alpha-AMYLASE AND A PROCESS FOR ITS PREPARATION
FR2256248A1 (en) * 1973-12-28 1975-07-25 Staley Mfg Co A E Non-retrograded thinned starch hydrolyzates - with reduced alpha amylase requirements
JPS5144690A (en) * 1974-10-14 1976-04-16 Nagase & Co Ltd Tainetsuseiarufua amiraazeno seizoho
FR2304675A1 (fr) * 1975-03-21 1976-10-15 Cpc International Inc Procede de production de levulose a partir d'amidon granulaire

Also Published As

Publication number Publication date
CA1081633A (en) 1980-07-15
FI771240A (es) 1977-10-20
YU40476B (en) 1986-02-28
DK169877A (da) 1977-10-20
NL7704280A (nl) 1977-10-21
DE2717333C2 (de) 1986-10-16
FI58157B (fi) 1980-08-29
NZ183818A (en) 1980-05-08
DK149157C (da) 1986-07-14
AU513863B2 (en) 1981-01-08
BR7702475A (pt) 1978-05-02
IT1080353B (it) 1985-05-16
FR2348930B1 (es) 1983-02-11
FI58157C (fi) 1980-12-10
JPS52151790A (en) 1977-12-16
AR218023A1 (es) 1980-05-15
YU99877A (en) 1982-08-31
MY8000095A (en) 1980-12-31
AU2422677A (en) 1978-10-19
JPS5752837B2 (es) 1982-11-10
DE2717333A1 (de) 1977-11-10
DK149157B (da) 1986-02-17
FR2348930A1 (fr) 1977-11-18
GB1539694A (en) 1979-01-31

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