ES2566053T3 - Procedimiento para la producción in vitro de tejido cartilaginoso u óseo tridimensional vital - Google Patents
Procedimiento para la producción in vitro de tejido cartilaginoso u óseo tridimensional vital Download PDFInfo
- Publication number
- ES2566053T3 ES2566053T3 ES01913858.5T ES01913858T ES2566053T3 ES 2566053 T3 ES2566053 T3 ES 2566053T3 ES 01913858 T ES01913858 T ES 01913858T ES 2566053 T3 ES2566053 T3 ES 2566053T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- cartilaginous
- procedure
- bone tissue
- vitro production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Rheumatology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Orthopedic Medicine & Surgery (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physical Education & Sports Medicine (AREA)
- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Procedimiento para la producción in vitro de un agregado celular a base de tejido cartilaginoso u óseo tridimensional, vital, que es un esferoide, caracterizado por que células cartilaginosas, células óseas o células madre mesenquimales obtenidas de un organismo humano o animal se cultivan en recipientes de cultivo celular con superficie hidrófoba y fondos que se estrechan en forma de cultivo en suspensión bajo la adición de suero autógeno o suero autólogo y sin factores de crecimiento y sin antibióticos y sin fungiestáticos hasta que resulta un agregado celular, que es un esferoide y que contiene hasta al menos 40% en volumen de matriz extracelular (ECM) en la que están embutidas células diferenciadas, y que presenta una zona externa en la que están presentes células susceptibles de proliferación y de migración.
Description
Claims (1)
-
imagen1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10013223A DE10013223C2 (de) | 2000-03-13 | 2000-03-13 | Verfahren zur in vitro-Herstellung von dreidimensionalem, vitalem Knorpel- oder Knochengewebe und dessen Verwendung als Transplantationsmaterial |
DE10013223 | 2000-03-13 | ||
PCT/EP2001/002698 WO2001068811A2 (de) | 2000-03-13 | 2001-03-09 | Verfahren zur in vitro-herstellung von dreidimensionalem, vitalem knorpel- oder knochengewebe und dessen verwendung als transplantationsmaterial |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2566053T3 true ES2566053T3 (es) | 2016-04-08 |
Family
ID=7635247
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES01913858.5T Expired - Lifetime ES2566053T3 (es) | 2000-03-13 | 2001-03-09 | Procedimiento para la producción in vitro de tejido cartilaginoso u óseo tridimensional vital |
Country Status (9)
Country | Link |
---|---|
US (1) | US7887843B2 (es) |
EP (1) | EP1265986B1 (es) |
AU (1) | AU2001239287A1 (es) |
CA (1) | CA2403120A1 (es) |
CY (1) | CY1117464T1 (es) |
DE (1) | DE10013223C2 (es) |
DK (1) | DK1265986T3 (es) |
ES (1) | ES2566053T3 (es) |
WO (1) | WO2001068811A2 (es) |
Families Citing this family (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10222896A1 (de) * | 2002-05-23 | 2003-12-11 | Biotissue Technologies Ag | Verfahren zur Herstellung eines dreidimensionalen und flächigen Gewebetransplantats |
US7622562B2 (en) | 2002-06-26 | 2009-11-24 | Zimmer Orthobiologics, Inc. | Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue |
DE10253066A1 (de) * | 2002-11-07 | 2004-05-27 | Co.Don Aktiengesellschaft | Gewebeersatzstruktur, Verfahren zur Modifikation einer Gewebeläsion und Verwendung von vorgeformtem dreidimensionalem Gewebe als Lieferant von Botenstoffen und/oder Strukturbausteinen |
WO2004110512A2 (en) * | 2003-06-12 | 2004-12-23 | Interface Biotech A/S | A method for cell implantation |
US7744869B2 (en) | 2003-08-20 | 2010-06-29 | Ebi, Llc | Methods of treatment using electromagnetic field stimulated mesenchymal stem cells |
ES2404682T3 (es) | 2003-12-11 | 2013-05-28 | Isto Technologies Inc. | Sistema de cartílago particulado |
US8148150B2 (en) * | 2003-12-12 | 2012-04-03 | Co.Don Ag | Method for the production of intervertebral disk cell transplants and their use as transplantation material |
US8241905B2 (en) | 2004-02-24 | 2012-08-14 | The Curators Of The University Of Missouri | Self-assembling cell aggregates and methods of making engineered tissue using the same |
JP4044579B2 (ja) * | 2005-08-02 | 2008-02-06 | 株式会社Pgリサーチ | 人工軟骨組織 |
EP1916964A4 (en) | 2005-08-26 | 2015-11-04 | Zimmer Inc | IMPLANTS AND METHODS FOR THE REPAIR, REPLACEMENT AND TREATMENT OF JOINT DISEASES |
EP1764117A1 (en) * | 2005-09-20 | 2007-03-21 | Zimmer GmbH | Implant for the repair of a cartilage defect and method for manufacturing the implant |
US20070093905A1 (en) * | 2005-10-21 | 2007-04-26 | O'neil Michael J | Degenerative disc regeneration techniques |
US20080166329A1 (en) * | 2005-10-24 | 2008-07-10 | Hsing-Wen Sung | Medical device and methods for living cell injection |
JP5039715B2 (ja) | 2006-01-24 | 2012-10-03 | ブラウン ユニバーシティ | 細胞凝集及び封入デバイス及び方法 |
KR20080091832A (ko) * | 2006-01-30 | 2008-10-14 | 유니버시티 오브 버지니아 페이턴트 파운데이션 | 중간엽 줄기 세포 응집물의 제조 및 특성화 방법, 및 이의 용도 |
US8835170B2 (en) * | 2006-10-06 | 2014-09-16 | University Of Virginia Patent Foundation | Methods and compositions useful for diabetic wound healing |
US8163549B2 (en) | 2006-12-20 | 2012-04-24 | Zimmer Orthobiologics, Inc. | Method of obtaining viable small tissue particles and use for tissue repair |
US20090012629A1 (en) | 2007-04-12 | 2009-01-08 | Isto Technologies, Inc. | Compositions and methods for tissue repair |
AU2009271223B2 (en) | 2008-06-24 | 2013-05-16 | The Curators Of The University Of Missouri | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
EP2339990A4 (en) * | 2008-07-06 | 2013-01-23 | Univ Missouri | OSTEO-CARTILAGINOUS IMPLANTS, METHODS OF ARTHROPLASTY, DEVICES AND ASSOCIATED SYSTEMS |
US20100098739A1 (en) * | 2008-10-20 | 2010-04-22 | University Of Virginia Patent Foundation | Compositions and methods for modular soft tissue repair |
TW201022435A (en) * | 2008-12-02 | 2010-06-16 | Univ Nat Taiwan | Method for transferring a cell onto a carrier and the applications thereof |
US8501476B2 (en) * | 2009-10-07 | 2013-08-06 | Brown University | Assays and methods for fusing cell aggregates to form proto-tissues |
CA2788514A1 (en) | 2010-02-02 | 2011-08-11 | Gabor Forgacs | Engineered biological nerve graft fabrication and application thereof |
CN105496601A (zh) | 2010-10-21 | 2016-04-20 | 奥加诺沃公司 | 用于制造组织的装置、***和方法 |
CN103747693B (zh) | 2011-07-26 | 2017-08-01 | 密苏里大学董事会 | 可食用工程肉 |
US9243278B2 (en) | 2011-09-22 | 2016-01-26 | Brown University | Mechanotransduction by the synergistic action of heterotypic cell interactions |
US9468680B2 (en) | 2011-09-22 | 2016-10-18 | Brown University | Differential effects of drugs on transport in a multi-layer 3D spheroid model |
US9499779B2 (en) | 2012-04-20 | 2016-11-22 | Organovo, Inc. | Devices, systems, and methods for the fabrication of tissue utilizing UV cross-linking |
EP2677027A1 (de) | 2012-06-21 | 2013-12-25 | Hochschule Lausitz | Verfahren zur Herstellung von funktionalem Fusionsgewebe aus humanen Chondrozyten |
US20140178343A1 (en) | 2012-12-21 | 2014-06-26 | Jian Q. Yao | Supports and methods for promoting integration of cartilage tissue explants |
US10072241B2 (en) | 2013-03-13 | 2018-09-11 | Innovative Surface Technologies, Inc. | Conical devices for three-dimensional aggregate(s) of eukaryotic cells |
US9442105B2 (en) | 2013-03-15 | 2016-09-13 | Organovo, Inc. | Engineered liver tissues, arrays thereof, and methods of making the same |
KR20160036619A (ko) | 2013-07-31 | 2016-04-04 | 오가노보, 인크. | 조직을 제작하기 위한 자동화 장치, 시스템 및 방법 |
WO2015038988A1 (en) | 2013-09-13 | 2015-03-19 | Modern Meadow, Inc. | Edible and animal-product-free microcarriers for engineered meat |
JP2017505138A (ja) | 2014-02-05 | 2017-02-16 | モダン メドー インコーポレイテッド | 培養筋細胞から形成される乾燥食品 |
DK3126490T3 (da) | 2014-04-04 | 2021-01-04 | Organovo Inc | Kunstigt tredimensionalt brystvæv, fedtvæv og tumorsygdomsmodel |
RU2017110275A (ru) | 2014-10-06 | 2018-11-15 | Органово, Инк. | Сконструированные ткани почки, матрицы на их основе и способы их получения |
US20160122723A1 (en) | 2014-11-05 | 2016-05-05 | Organovo, Inc. | Engineered three-dimensional skin tissues, arrays thereof, and methods of making the same |
DE102014222898A1 (de) * | 2014-11-10 | 2016-05-12 | Universität Konstanz | Herstellung und Isolierung einer nativen humanen dermalen extrazellulären Matrix |
US11913166B2 (en) | 2015-09-21 | 2024-02-27 | Modern Meadow, Inc. | Fiber reinforced tissue composites |
KR101733137B1 (ko) * | 2015-12-30 | 2017-05-08 | (주)엑셀세라퓨틱스 | 연골조직 제조를 위한 3차원 오가노이드 블록 제작 방법 |
KR20170096095A (ko) | 2016-02-15 | 2017-08-23 | 브렌던 패트릭 퍼셀 | 콜라겐 피브릴을 함유하는 생제작된 물질 |
CN110167609A (zh) | 2016-11-10 | 2019-08-23 | 奥加诺沃公司 | 生物打印的毛囊及其用途 |
IL270836B2 (en) * | 2017-05-30 | 2023-12-01 | Univ Leuven Kath | Functional Hinduism of bone organs |
EP3417888A1 (de) * | 2017-06-25 | 2018-12-26 | co.don AG | Verfahren zum herstellen von transplantierbarem knorpelgewebe |
AU2018253595A1 (en) | 2017-11-13 | 2019-05-30 | Modern Meadow, Inc. | Biofabricated leather articles having zonal properties |
RU2691916C1 (ru) * | 2018-05-03 | 2019-06-18 | Федеральное государственное бюджетное учреждение "Новосибирский научно-исследовательский институт травматологии и ортопедии им. Я.Л. Цивьяна" Министерства здравоохранения Российской Федерации (ФГБУ "ННИИТО им. Я.Л. Цивьяна" Минздрава России) | Способ хирургического лечения деформирующего остеоартроза коленного сустава |
EP3704202A4 (en) | 2019-01-17 | 2020-12-16 | Modern Meadow, Inc. | LAYERED COLLAGEN MATERIALS AND METHODS FOR MANUFACTURING THEM |
EP3744831A1 (en) | 2019-05-31 | 2020-12-02 | co.don AG | Method for cultivation of cartilage and spheroids thereof |
CN111019888A (zh) * | 2020-01-10 | 2020-04-17 | 中怡(深圳)医疗科技集团有限公司 | 一种基于鲟鱼来源的原代软骨细胞提取方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5723331A (en) * | 1994-05-05 | 1998-03-03 | Genzyme Corporation | Methods and compositions for the repair of articular cartilage defects in mammals |
US5655546A (en) * | 1995-06-07 | 1997-08-12 | Halpern; Alan A. | Method for cartilage repair |
DE19540487A1 (de) * | 1995-10-20 | 1997-04-24 | Olaf Schultz | Zellinteraktionssystem zur Induktion künstlicher Gewebe |
CA2198978A1 (en) * | 1996-03-01 | 1997-09-01 | Clemens Antoni Van Blitterswijk | Method for the in vitro production of bone |
US6811776B2 (en) * | 2000-12-27 | 2004-11-02 | The Regents Of The University Of Michigan | Process for ex vivo formation of mammalian bone and uses thereof |
-
2000
- 2000-03-13 DE DE10013223A patent/DE10013223C2/de not_active Expired - Lifetime
-
2001
- 2001-03-09 WO PCT/EP2001/002698 patent/WO2001068811A2/de active Application Filing
- 2001-03-09 EP EP01913858.5A patent/EP1265986B1/de not_active Expired - Lifetime
- 2001-03-09 US US10/239,701 patent/US7887843B2/en not_active Expired - Fee Related
- 2001-03-09 AU AU2001239287A patent/AU2001239287A1/en not_active Abandoned
- 2001-03-09 ES ES01913858.5T patent/ES2566053T3/es not_active Expired - Lifetime
- 2001-03-09 DK DK01913858.5T patent/DK1265986T3/en active
- 2001-03-09 CA CA002403120A patent/CA2403120A1/en not_active Abandoned
-
2016
- 2016-03-23 CY CY20161100246T patent/CY1117464T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
EP1265986A2 (de) | 2002-12-18 |
DE10013223A1 (de) | 2001-10-11 |
CA2403120A1 (en) | 2002-09-13 |
DK1265986T3 (en) | 2016-04-04 |
WO2001068811A2 (de) | 2001-09-20 |
EP1265986B1 (de) | 2016-02-24 |
US20030153078A1 (en) | 2003-08-14 |
WO2001068811A3 (de) | 2001-12-27 |
CY1117464T1 (el) | 2017-04-26 |
DE10013223C2 (de) | 2002-07-18 |
US7887843B2 (en) | 2011-02-15 |
AU2001239287A1 (en) | 2001-09-24 |
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