ES2180207T3 - Deteccion e identificacion del papilomavirus humano por medio de pcr y de hibridacion inversa de tipo especifico. - Google Patents
Deteccion e identificacion del papilomavirus humano por medio de pcr y de hibridacion inversa de tipo especifico.Info
- Publication number
- ES2180207T3 ES2180207T3 ES98951392T ES98951392T ES2180207T3 ES 2180207 T3 ES2180207 T3 ES 2180207T3 ES 98951392 T ES98951392 T ES 98951392T ES 98951392 T ES98951392 T ES 98951392T ES 2180207 T3 ES2180207 T3 ES 2180207T3
- Authority
- ES
- Spain
- Prior art keywords
- hpv
- identification
- detection
- pcr
- probes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Procedimiento para la detección y/o identificación del HPV que se encuentra en una muestra biológica, que comprende las etapas siguientes: (i) amplificación de un fragmento de ácido polinucleico del HPV mediante la utilización de un iniciador de extremo 5'' que se hibridice específicamente con la región A ó B del genoma de por lo menos un tipo de HPV, indicándose en la figura 1 dichas regiones A y B, y un iniciador de extremo 3'' que se hibridice específicamente con la región C del genoma de por lo menos un tipo de HPV, indicándose en la figura 1 dicha región C; (ii) hibridación de los fragmentos amplificados de la etapa (i) con por lo menos una sonda capaz de hibridizarse específicamente con la región D de por lo menos un tipo de HPV, indicándose dicha región D en la figura 1.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97870136 | 1997-09-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2180207T3 true ES2180207T3 (es) | 2003-02-01 |
Family
ID=8231037
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES98951392T Expired - Lifetime ES2180207T3 (es) | 1997-09-16 | 1998-09-14 | Deteccion e identificacion del papilomavirus humano por medio de pcr y de hibridacion inversa de tipo especifico. |
Country Status (11)
Country | Link |
---|---|
US (2) | US6482588B1 (es) |
EP (2) | EP1012348B1 (es) |
AT (1) | ATE219153T1 (es) |
AU (1) | AU9743398A (es) |
CA (1) | CA2302146C (es) |
DE (1) | DE69806024T2 (es) |
DK (1) | DK1012348T3 (es) |
ES (1) | ES2180207T3 (es) |
HK (1) | HK1031238A1 (es) |
PT (1) | PT1012348E (es) |
WO (1) | WO1999014377A2 (es) |
Families Citing this family (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100382703B1 (ko) | 2000-03-15 | 2003-05-09 | 주식회사 바이오메드랩 | 인유두종바이러스의 유전형 진단키트 및 상기 진단키트의제조방법 |
US20020155427A1 (en) * | 2000-04-03 | 2002-10-24 | Cohenford Menashi A. | Detection and typing of human papillomavirus using PNA probes |
US7439016B1 (en) * | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
GB0018050D0 (en) * | 2000-07-21 | 2000-09-13 | Norchip As | Detection of human papillomavirus mRNA |
JP2004538010A (ja) * | 2001-08-08 | 2004-12-24 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | アッセイ |
KR100452163B1 (ko) * | 2001-09-14 | 2004-10-12 | 주식회사 바이오메드랩 | 인유두종 바이러스의 감염을 진단하기 위한 유전형 분석키트 |
EP1302550A1 (en) * | 2001-10-10 | 2003-04-16 | King Car Food Industrial Co., Ltd. | Method and detector for identifying subtypes of human papilloma viruses |
HUP0200981A3 (en) * | 2002-03-14 | 2004-06-28 | Genoid Kft | Pcr reactions with hybridizing probes using primers with broad genotype-specificity for detection of human papilloma-viruses and typing amplificates by using specifically hybridizing oligonucleotides |
EP1359192A1 (en) | 2002-04-30 | 2003-11-05 | Solvay Polyolefins Europe-Belgium (Société Anonyme) | Polyethylene pipe resins |
US7704965B2 (en) | 2002-06-26 | 2010-04-27 | The Penn State Research Foundation | Methods and materials for treating human papillomavirus infections |
US7452712B2 (en) | 2002-07-30 | 2008-11-18 | Applied Biosystems Inc. | Sample block apparatus and method of maintaining a microcard on a sample block |
EP1403384A1 (en) * | 2002-09-26 | 2004-03-31 | Stichting Researchfonds Pathologie | Method for detecting and typing of cutaneous HPV and primers and probes for use therein |
EP2031070B1 (en) | 2002-12-04 | 2013-07-17 | Life Technologies Corporation | Multiplex amplification of polynucleotides |
US20060024690A1 (en) * | 2003-09-19 | 2006-02-02 | Kao H P | Normalization of data using controls |
US20050112634A1 (en) * | 2003-09-19 | 2005-05-26 | Woudenberg Timothy M. | High density sequence detection methods and apparatus |
US7527948B2 (en) | 2003-09-25 | 2009-05-05 | Third Wave Technologies, Inc. | Detection of HPV |
WO2005033333A2 (en) | 2003-10-07 | 2005-04-14 | Dako Denmark A/S | Methods and compositions for the diagnosis of cancer |
WO2005085475A1 (en) | 2004-03-01 | 2005-09-15 | Applera Corporation | Methods, compositions and kits for use in polynucleotide amplification |
US7482142B1 (en) | 2004-05-07 | 2009-01-27 | Roche Molecular Systems, Inc. | High-risk human papillomavirus detection |
US20050255485A1 (en) * | 2004-05-14 | 2005-11-17 | Livak Kenneth J | Detection of gene duplications |
US20060003337A1 (en) * | 2004-06-30 | 2006-01-05 | John Brandis | Detection of small RNAS |
US7670774B2 (en) * | 2004-10-04 | 2010-03-02 | Goodgene Inc. | Probe of human papillomavirus and DNA chip comprising the same |
US7901883B2 (en) * | 2004-12-10 | 2011-03-08 | Genera Biosystems Limited | Human papilloma virus (HPV) detection using nucleic acid probes, microbeads and fluorescent-activated cell sorter (FACS) |
GB0500996D0 (en) * | 2005-01-18 | 2005-02-23 | Delfts Diagnostic Labaratory B | Detection method and materials therefor |
US8927208B2 (en) * | 2005-03-30 | 2015-01-06 | Labo Bio-Medical Investments B.V. | Identification of beta-papillomavirus DNA by type-specific reverse hybridization |
US20070031826A1 (en) * | 2005-08-05 | 2007-02-08 | My Gene | Diagnostic kit for determining the genotype of a human papilloma virus and method of using thereof |
GB0516145D0 (en) * | 2005-08-05 | 2005-09-14 | Genomica Sau | In vitro diagnostic kit for identification of human papillomavirus in clinical samples |
KR20080084809A (ko) * | 2005-11-15 | 2008-09-19 | 게노이드 케이에프티. | 병원체를 검출하는 방법 |
US8841069B2 (en) * | 2005-12-29 | 2014-09-23 | Korea Materials & Analysis Corporation | Dendron-mediated DNA virus detection |
KR100702415B1 (ko) * | 2006-03-03 | 2007-04-09 | 안웅식 | 올리고 핵산 탐침 비드 어레이를 이용한 인유두종바이러스검출 키트 및 방법 |
EP3167900B1 (en) | 2006-03-29 | 2018-11-21 | Merial Limited | Vaccine against streptococci |
ZA200901744B (en) * | 2006-08-11 | 2010-10-27 | Chu Sainte Justine | Oligonucleotides for discriminating related nucleic acid sequences |
US8080643B2 (en) * | 2006-09-05 | 2011-12-20 | Third Wave Technologies, Inc. | HPV primers |
ATE518958T1 (de) | 2007-01-30 | 2011-08-15 | Transgene Sa | Zur impfung verwendetes papillomavirus-e2- polypeptid |
GB0702557D0 (en) * | 2007-02-09 | 2007-03-21 | Health Prot Agency | Detection of human papillomavirus |
ES2403729T3 (es) * | 2007-04-05 | 2013-05-21 | Genera Biosystems Limited | Composiciones y métodos de detección |
EP1997914A1 (en) * | 2007-06-01 | 2008-12-03 | Università Degli Studi Di Milano - Bicocca | Identification and quantification of oncogenic HPV nucleic acids by means of real-time PCR assays |
ES2584228T3 (es) * | 2007-11-01 | 2016-09-26 | Self-Screen B.V. | Nuevo método de detección para los HPV cervicales |
US9090948B2 (en) | 2008-09-30 | 2015-07-28 | Abbott Molecular Inc. | Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples |
GB0820822D0 (en) | 2008-11-13 | 2008-12-24 | Inst Catala D Oncologia | Novel product and processes |
WO2010058357A1 (en) | 2008-11-19 | 2010-05-27 | Diagcor Bioscience Incorporation Ltd. | Nucleotide sequences, methods and kits for detecting hpv |
NO330943B1 (no) * | 2009-04-30 | 2011-08-22 | Unilabs Telelabs As | En metode for detektering og/eller typebestemmelse og/eller kvantifisering av human papillomavirus (HPV) type, primere og prober derav samt diagnostisk sett og anvendelse derav. |
CN102497880A (zh) | 2009-06-25 | 2012-06-13 | 葛兰素史密丝克莱恩生物有限公司 | 新的人***状瘤病毒(hpv)蛋白构建体及其在预防hpv疾病中的用途 |
ES2357821B8 (es) * | 2009-07-07 | 2012-03-05 | Universidad Politécnica De Cartagena | Método para la detección y determinación de carga viral del virus del papiloma humano. |
JP6153866B2 (ja) | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー. | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
EP2703496A1 (en) | 2012-08-31 | 2014-03-05 | Innogenetics N.V. | Combination of PCR primers and a method for detection and identification of Human Papillomavirus (HPV) |
WO2014134607A1 (en) * | 2013-03-01 | 2014-09-04 | The Johns Hopkins University | Dual sequence-capture method for quantifying trans renal hpv dna in urine |
GB201511470D0 (en) * | 2015-06-30 | 2015-08-12 | Cellcall Kft | HPV detection method |
CN106939359A (zh) * | 2017-05-17 | 2017-07-11 | 上海市第十人民医院 | 一种lamp法检测hpv常见亚型的引物组及检测体系 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5182377A (en) * | 1988-09-09 | 1993-01-26 | Hoffmann-La Roche Inc. | Probes for detection of human papillomavirus |
US5639871A (en) * | 1988-09-09 | 1997-06-17 | Roche Molecular Systems, Inc. | Detection of human papillomavirus by the polymerase chain reaction |
US5447839A (en) * | 1988-09-09 | 1995-09-05 | Hoffmann-La Roche Inc. | Detection of human papillomavirus by the polymerase chain reaction |
NL9000134A (nl) * | 1990-01-19 | 1991-08-16 | Stichting Res Fonds Pathologie | Primers en werkwijze voor het detecteren van humaan papilloma virus genotypen m.b.v. pcr. |
-
1998
- 1998-09-14 WO PCT/EP1998/005829 patent/WO1999014377A2/en active IP Right Grant
- 1998-09-14 DK DK98951392T patent/DK1012348T3/da active
- 1998-09-14 AT AT98951392T patent/ATE219153T1/de active
- 1998-09-14 EP EP98951392A patent/EP1012348B1/en not_active Expired - Lifetime
- 1998-09-14 EP EP01204423A patent/EP1201771A3/en not_active Withdrawn
- 1998-09-14 PT PT98951392T patent/PT1012348E/pt unknown
- 1998-09-14 ES ES98951392T patent/ES2180207T3/es not_active Expired - Lifetime
- 1998-09-14 DE DE69806024T patent/DE69806024T2/de not_active Expired - Lifetime
- 1998-09-14 AU AU97433/98A patent/AU9743398A/en not_active Abandoned
- 1998-09-14 CA CA2302146A patent/CA2302146C/en not_active Expired - Lifetime
-
2000
- 2000-03-16 US US09/527,030 patent/US6482588B1/en not_active Expired - Lifetime
- 2000-12-21 HK HK00108367A patent/HK1031238A1/xx not_active IP Right Cessation
-
2002
- 2002-09-11 US US10/241,780 patent/US20030165821A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1201771A3 (en) | 2002-10-23 |
WO1999014377A2 (en) | 1999-03-25 |
DE69806024D1 (de) | 2002-07-18 |
HK1031238A1 (en) | 2001-06-08 |
AU9743398A (en) | 1999-04-05 |
EP1012348A2 (en) | 2000-06-28 |
EP1012348B1 (en) | 2002-06-12 |
DK1012348T3 (da) | 2002-10-14 |
EP1201771A2 (en) | 2002-05-02 |
WO1999014377A3 (en) | 1999-06-17 |
CA2302146C (en) | 2010-07-13 |
DE69806024T2 (de) | 2003-02-13 |
PT1012348E (pt) | 2002-11-29 |
ATE219153T1 (de) | 2002-06-15 |
US6482588B1 (en) | 2002-11-19 |
CA2302146A1 (en) | 1999-03-25 |
US20030165821A1 (en) | 2003-09-04 |
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