ES2357821B8 - Método para la detección y determinación de carga viral del virus del papiloma humano. - Google Patents
Método para la detección y determinación de carga viral del virus del papiloma humano. Download PDFInfo
- Publication number
- ES2357821B8 ES2357821B8 ES200930425A ES200930425A ES2357821B8 ES 2357821 B8 ES2357821 B8 ES 2357821B8 ES 200930425 A ES200930425 A ES 200930425A ES 200930425 A ES200930425 A ES 200930425A ES 2357821 B8 ES2357821 B8 ES 2357821B8
- Authority
- ES
- Spain
- Prior art keywords
- determination
- detection
- hpv
- amplifying
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000003612 virological effect Effects 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 2
- 241000700605 Viruses Species 0.000 title 1
- 241000701806 Human papillomavirus Species 0.000 abstract 5
- 101150075239 L1 gene Proteins 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical group O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método para la detección y determinación de carga viral del virus del papiloma humano.#Un par de cebadores degenerados capaces de amplificar un fragmento del gen L1 de varios genotipos del virus del papiloma humano (VPH) y un método de detección y de cuantificación de la carga viral del VPH en muestras biológicas aisladas, que comprende: la extracción de ADN de una muestra biológica aislada, poner en contacto la muestra con una mezcla de reacción que contiene al menos un fluorocromo y los cebadores degenerados de la invención, amplificar un fragmento del gen L1 del VPH, medir la fluorescencia obtenida en el paso de amplificación y comparar el valor de fluorescencia obtenido con una secuencia control.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200930425A ES2357821B8 (es) | 2009-07-07 | 2009-07-07 | Método para la detección y determinación de carga viral del virus del papiloma humano. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200930425A ES2357821B8 (es) | 2009-07-07 | 2009-07-07 | Método para la detección y determinación de carga viral del virus del papiloma humano. |
Publications (3)
Publication Number | Publication Date |
---|---|
ES2357821A1 ES2357821A1 (es) | 2011-05-03 |
ES2357821B1 ES2357821B1 (es) | 2012-01-13 |
ES2357821B8 true ES2357821B8 (es) | 2012-03-05 |
Family
ID=43859198
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200930425A Expired - Fee Related ES2357821B8 (es) | 2009-07-07 | 2009-07-07 | Método para la detección y determinación de carga viral del virus del papiloma humano. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2357821B8 (es) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999014377A2 (en) * | 1997-09-16 | 1999-03-25 | Innogenetics N.V. | Detection and identification of human papillomavirus by pcr and type-specific reverse hybridization |
-
2009
- 2009-07-07 ES ES200930425A patent/ES2357821B8/es not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2357821B1 (es) | 2012-01-13 |
ES2357821A1 (es) | 2011-05-03 |
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Legal Events
Date | Code | Title | Description |
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FG2A | Definitive protection |
Ref document number: 2357821 Country of ref document: ES Kind code of ref document: B1 Effective date: 20120113 |
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FD2A | Announcement of lapse in spain |
Effective date: 20190611 |