EP1171240B2 - Pointe de pipette, dispositif a pipetter et combinaison de pointe de pipette et de dispositif de pipette - Google Patents

Pointe de pipette, dispositif a pipetter et combinaison de pointe de pipette et de dispositif de pipette Download PDF

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Publication number
EP1171240B2
EP1171240B2 EP00926950A EP00926950A EP1171240B2 EP 1171240 B2 EP1171240 B2 EP 1171240B2 EP 00926950 A EP00926950 A EP 00926950A EP 00926950 A EP00926950 A EP 00926950A EP 1171240 B2 EP1171240 B2 EP 1171240B2
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EP
European Patent Office
Prior art keywords
pipette tip
pipette
coupling
axial
unit according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP00926950A
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German (de)
English (en)
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EP1171240B1 (fr
EP1171240A1 (fr
Inventor
Armin Panzer
Johann L. Camenisch
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Hamilton Bonaduz AG
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Hamilton Bonaduz AG
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Application filed by Hamilton Bonaduz AG filed Critical Hamilton Bonaduz AG
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Publication of EP1171240B1 publication Critical patent/EP1171240B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means

Definitions

  • the invention relates to the attachment of a pipette tip to a pipetting device.
  • Pipetting devices are used for example in the field of molecular biology or medical analysis for liquid transfer.
  • special pipette tips are often used, which are attached to the pipetting device and intended for single use.
  • Such disposable tips are also known by the name "Tip".
  • the pipetting device may be a manual pipetting device having only a single pipetting unit.
  • pipetting machines or so-called pipetting robots are often used which have a multiplicity of pipetting units arranged in a row or in a matrix. With such automatic pipetting can be simultaneously sucked from a variety of vessels samples and released elsewhere.
  • the pipette tips have hitherto (see, for example, US-A-5,851,491) mostly been designed with a conically widening jacket area with which they are placed on a correspondingly conically shaped coupling attachment of the pipetting device, or rather of the respective pipetting unit.
  • the pipette tip is pressed so tightly on the coupling lug that adjusts a frictional interference fit between the pipette tip and the coupling lug.
  • the material of the pipette tip has a certain elasticity, which causes the pipette tip to expand when being pressed onto the coupling cone.
  • a pipetting unit described in EP 0 760 255 A1 has an adjustable pretensioning element in the form of an inflatable rubber sleeve, the edge of which facing the pipette tip abutting against a radial axial abutment surface of the pipette tip. Since the edge of the rubber boot is more or less elastic, but can not be spoken of a precise adhered Ankoppelwolf in the attack trap. If the pipette tip is not pushed up to the immediate stop on the edge of the rubber sleeve, but its stop surface has a more or less large clearance from the rubber cuff, this distance remains more or less preserved when inflating the rubber boot, which drastically shifts the positioning accuracy.
  • the push-on end of the pipette tip in turn only has a radial axial stop surface, but no surface component of an engagement surface facing away from the pin-up direction for an O-ring serving as a biasing member.
  • the O-ring is not adjustable via an actuator of the pipette between a bias state and a release state.
  • a defined position of the pipette tip relative to the coupling approach is of particular importance, in particular in pipetting machines having a multiplicity of pipetting units.
  • Different positions of the pipette tips in pipetting machines with several tens or even several hundred pipetting units may, for example during liquid aspiration, cause some pipette tips to dive properly into their associated vessels or in wells of a microtiter plate, while leaving other pipette tips above the liquid level in the vessels or wells.
  • the object of the invention is therefore to show a way, as in particular in automatic pipetting with a variety of pipetting the dosing accuracy can be improved during liquid transfer.
  • the present invention provides a pipetting unit according to claim 1.
  • the pipette tip for attachment to a pipetting device
  • the pipette tip has a jacket and a passage opening enclosed by the jacket, the passage opening extending along a longitudinal axis between a front end of the pipette tip intended for immersion in a medium to be pipetted and a plug-in end of the pipette tip opposite in the axial direction and wherein the pipette tip near the Aufstekkendes has a coupling region for coupling with a coupling lug of the pipetting device.
  • the jacket carries in the coupling region axial positioning means, which are intended to cooperate with complementary counter axial positioning means of the coupling lug and define together with the counter Axialposition réellesstoffn an axial coupling position of the pipette tip on the pipetting device.
  • the axial positioning means make it possible to obtain a predetermined defined axial position of the pipette tip relative to the pipetting device each time the pipette tip is attached to the pipetting device. This enables positionally exact and positionally identical equipping of all pipetting units of a pipetting machine with pipette tips.
  • the pipette tip with its push-on end will be able to be pushed onto the coupling projection in advance, for which reason the axial positioning elements will expediently be arranged at least in part on the inner circumference of the jacket at an axial distance from the insertion end of the pipette tip.
  • the shell inner circumference may have an envelope formed essentially of cylindrical sections, at least in the coupling region.
  • the axial positioning means may comprise at least one axial stop arranged on the shell, which is intended to cooperate with a complementary counter-stop of the coupling lug.
  • the axial stop engages with the counterstop of the coupling lug, resulting in a defined axial position of the pipette tip.
  • the coupling lug also carries an elastically deformable O-ring, which comes through axial crushing and consequent radial expansion in frictional engagement with a Dichülumblee the pipette tip, a particularly simple, yet highly accurate attachment option for the pipette tip is given to the pipetting.
  • the axial stop can be formed by an axial step shoulder of the inner circumference of the circumference, which connects a first cylindrical inner circumferential section of larger diameter closer to the upper end of the Aufstekkende with a second cylindrical outer circumferential cylindrical section of smaller diameter.
  • the diameter reduction from the first to the second shell inner peripheral portion, effected by the stepped shoulder can be between 0.8 and 1.2 mm, preferably between 0.9 and 1.1 mm, most preferably about 1.0 mm.
  • the diameter of the first shell inner peripheral portion may be between 6.5 and 7.1 mm, preferably between 6.7 and 6.9 mm, most preferably about 6.8 mm.
  • the diameter of the second shell inner peripheral portion may be between 5.5 and 6.1 mm, preferably between 5.7 and 5.9 mm, most preferably about 5.8 mm. It is advisable to define a standard coupling interface between the pipette tip and the pipetting device, so that pipette tips with different receiving volumes for liquids to be pipetted, but with standardized coupling regions, can be combined with one and the same pipetting device.
  • the exact axial positioning of the pipette tip by its axial stop and the counter-stop of the coupling lug can be further improved in that the jacket in the coupling region has an engagement surface for a held on the pipetting device biasing member, which is intended, in the Ankoppelungs ein the pipette tip whose axial stop axially bias against the counter-stop of the coupling lug.
  • a structurally particularly simple solution can consist in that the engagement surface is at the same time designed for sealing engagement of a sealing element forming the biasing member of elastically deformable material, which serves to seal the pipette tip against the coupling approach.
  • the sealing element therefore not only assumes sealing function, but at the same time also pretensioning function.
  • For the sealing element can be used on known solutions.
  • the sealing element may be formed by an O-ring and according to US-A-5,063,790 be axially compressible by a squeezing device of the pipetting device. It can then be provided that the attack surface is designed and located on the jacket that in the Ankoppelungs ein the pipette tip, the sealing element in an axially uncompressed state substantially vorspannkraftindem engagement with the attack surface and in the course of an axial compression in vorspannkraftinden engagement with the attack surface passes.
  • the attack surface is formed on an incorporated into the shell inner circumference Ringnüt into which the sealing element of the coupling lug can "snap".
  • the engagement surface is preferably arranged axially between the axial stop and the second end of the pipette tip.
  • a pipetting apparatus having at least one pipetting unit having a pipetting channel extending along a channel axis and a coupling lug for coupling a pipette tip, in particular of the type described above, the coupling lug for interacting with axial positioning of the pipette tip certain complementary Jacob-Axialpositiontechniksstoff, which together with define an axial coupling position of the pipette tip on the pipetting unit to the axial positioning means.
  • the coupling lug can be plugged into the pipette tip ahead with a push-on end, it then being expedient to arrange the counter-axial positioning means at least partially on the outer circumference of the coupling lug at an axial distance from the push-on end.
  • the outer periphery of the coupling projection can have an envelope essentially formed by cylindrical sections, at least in the region of the coupling projection projecting into the pipette tip in the coupling position.
  • the counter-axial positioning means may comprise at least one complementary counter-abutment on the coupling tab intended to cooperate with an axial stop of the pipette tip.
  • This counter-stop can be formed by a stepped shoulder on the outer circumference of the coupling lug.
  • the coupling lug can carry a sealing element serving for sealing between the pipette tip and the coupling lug, in particular an O-sealing ring, of elastically deformable material, wherein the pipetting unit can be assigned a squeezing device for axially compressing the sealing element.
  • the sealing element can not only assume a sealing function, but also a biasing function, if the pipette tip has a correspondingly designed and localized engagement surface for the sealing element, which has a radial surface component and can be loaded with an axial force component of the sealing element.
  • a pipette tip of the type described above may be combined with a pipetting device of the type described above.
  • FIG. 1 One recognizes there a pipette tip 1, also referred to as a tip, with a jacket 5 which is rotationally symmetrical about a longitudinal axis 3 and which encloses a through-opening 7 passing axially through the pipette tip 1.
  • a coupling region 11 which serves for coupling to a not shown in Fig. 1 pipetting.
  • the Aufstekkende 9 opposite the pipette tip 1 has a mouth end 13, which is intended for immersion in the medium to be pipetted.
  • the sheath 5 of the pipette tip 1 has an outer circumference 15 and an inner circumference 17.
  • the inner periphery 17 has a cylindrical inner peripheral portion 19, which extends substantially from the Aufsteckende 9 to an annular stepped shoulder 21, to which a further cylindrical inner peripheral portion 23 connects.
  • the stepped shoulder 21 forms an axially directed stop surface.
  • a circumferentially extending annular groove 25 is incorporated in the jacket 5. This axially between the Aufsteckende 9 and the Stufanabsatz 21 arranged annular groove 25 has in the sectional view of FIG. 1, a groove contour which follows a circular arc.
  • the jacket 5 has a first conical wall section 27 and a second conical wall section 29, whose conicity is weaker than that of the wall section 27.
  • the jacket 5 is designed with a smaller wall thickness than in the coupling region 11. There, the greater wall thickness in the coupling region 11 increases the stability and rigidity of the pipette tip 1. This allows a secure and leak-free coupling of the pipette tip 1 to a pipetting device.
  • the inner diameter of the shell 5 in the region of the inner peripheral portion 19 may be about 6.8 mm and in the region of the inner peripheral portion 23 about 5.8 mm.
  • the stepped shoulder 21 can be arranged at an axial distance from the end 9 of about 5 mm.
  • the radius of curvature of the circular contour of the annular groove 25 may be about 0.6 mm.
  • the maximum radial depth of the annular groove 25 may be about 0.2 mm.
  • the axial distance between the step 21 and the axial center of the annular groove 25, so the lowest point, may be about 2.1 mm.
  • the pipetting unit 201 has a pipetting tube 205, which contains a pipetting channel 207, which runs along a channel axis 209 and continues in the coupling state of the pipette tip 101 on the pipetting unit 201 from the through opening 107 of the pipette tip 101.
  • the coupling projection 203 comprises a at the lower end of the pipette 205 fixedly attached thereto, for example, pressed or screwed coupling sleeve 211.
  • An O-ring 213 of elastically deformable and optionally electrically conductive material is pushed onto the pipette 205 and is located at the pipette tip 101 axially remote from the end face of the coupling sleeve 211.
  • a ferrule 215 is pushed onto the pipetting tube 205. This ferrule 215 is axially displaceable relative to the pipetting tube 205 and serves for the axial crushing of the O-ring seal 213.
  • the ferrule 215 can be actuated by means not shown actuating means.
  • actuating means may allow manual or automatic actuation of the ferrule 215.
  • the ferrule 215 may be hydraulically actuable. It is also conceivable to provide a screw drive for the adjustment of the ferrule 215, which can be actuated by hand, but which can also be actuated by means of an electric motor.
  • an ejector 217 which is movable axially relative to the pipetting tube 205, is also provided, which in the embodiment illustrated is designed as the ejector tube enclosing the ferrule 215 and the pipetting tube 205.
  • the manner of operation of the ejector 217 will be discussed later.
  • annular stepped shoulder 219 is formed, which abuts when provokekken the coupling projection 203 in the pipette tip 101 to the stepped shoulder 121 of the pipette tip 101.
  • the coupling sleeve 211 Towards the end of the coupling projection 203 leading to the insert, the coupling sleeve 211 has a cylindrical outer peripheral portion 221 whose diameter is matched to the diameter of the inner peripheral portion 123 of the pipette tip 101 in the sense of a smooth attachment of the pipette tip 101 to the coupling projection 203.
  • another cylindrical outer circumference section 223 of the coupling sleeve 211 adjoins the stepped shoulder 219, the diameter of which is matched to the diameter of the inner circumferential section 119 of the pipette tip 101 in the same sense.
  • Fig. 3 shows the state when the pipette tip 101 is attached and the O-ring seal 213 is still squeezed. It can be seen that the axial distance of the stepped shoulder 219 of the coupling sleeve 211 of the axial end face of the coupling sleeve 211, on which the O-ring 213 rests, is dimensioned such that in the Aufsteckposition shown in Fig. 3, the O-ring 213 axially something is offset from the annular groove 125. More specifically, the axial center of the annular groove 125 is offset slightly from the axial center of the uncompressed O-ring 213 toward the pipette tip 101.
  • Figs. 4 and 4a of which Fig. 4 shows the state in which the crushing of the O-ring seal 213 is completed and the final assembly position of the pipette tip 101 is made to the pipetting unit 201, and of which Figs 4a shows an enlarged section in the region of the O-sealing ring 213.
  • the ferrule 215 has at its end facing the O-ring 213 a leading tip 225 which, when approaching the ferrule 215 to the O-ring 213 between them and the pipetting tube 205 penetrates, so that the O-ring 213 is not only axially compressed, but also by the tip 225 is pressed radially slightly to the annular groove 125 out.
  • the O-ring 213 experiences a total increase in its outer diameter. Its radially pressed-out parts can escape into the free space through the annular groove 125, which is indicated by dashed lines in Fig. 4a.
  • This attack surface 127 has a radial component due to their curvature, so that when the O-ring 213 presses against the engagement surface 127, a force on the Pipette tip 101 is exerted with an axial component.
  • This axial force biases the stepped shoulder 121 of the pipette tip 101 axially against the stepped shoulder 219 of the coupling sleeve 211, whereby a secure axial hold of the pipette tip 101 on the pipetting unit 201 is brought about.
  • the ferrule 215 is raised axially from its squish position shown in Fig. 4, whereby the O-ring 213 relaxes again and withdraws from the annular groove 125.
  • the prestressing force exerted on the stepped shoulders 121, 219 in the coupling state according to FIG. 4 is thereby canceled. It finally turns back to the state shown in Fig. 3. Conveniently, in this condition shown in FIG.
  • the O-ring 213 will not be completely out of contact with the inner periphery 117 of the pipette tip 101, but in such engagement with the inner periphery 117 of the pipette tip 101 that the pipette tip 101 will not be detached from the pipetting unit 201 falls down.
  • the O-ring seal 213 may abut at the transition edge designated 129 between the annular groove 125 and the cylindrical inner peripheral portion 119 of the pipette tip 101.
  • this transition edge 129 must be moved past the O-ring seal 213, which is only possible with a simultaneous slight radial compression of the O-ring seal 213.
  • this radial compression of the O-ring 213 has a friction-increasing effect, which in itself would run counter to the requirement for a smooth stripping of the pipette tip 101 from the pipetting unit 201.
  • the cylindrical inner peripheral portion 119 of the pipette tip 101 situated above the annular groove 125 is preferably stepped, as can be seen in particular in FIG. 4a.
  • the transition edge 129 At a small axial distance from the transition edge 129, it has a step extension formed by a step 131, which causes a corresponding increase in diameter of this inner peripheral portion 119.
  • the ejector 217 is provided. This can be operated in various ways. For example, a hydraulic or electromotive operation of the ejector 217 is conceivable. Alternatively, a biasing spring, not shown, can be attached to the devaluation element 217, which is stretched when placing the pipette tip 101 on the pipetting unit 201, when the pipette tip 101 with its front end 109 pushes the ejector tube forming the ejector 217 upwards. Then, when the pinch seal of the O-ring 213 is released, this ejector bias spring can relax again.
  • the ejector biasing spring will be sized so that the force exerted by it in the tensioned state on the Abwerferrohr 217 force does not exceed the axial holding force of the O-ring 213. At the same time, it will be dimensioned so that the force exerted on the pipette tip 101 in the course of its relaxation is sufficient to move the edge 129 past the O-sealing ring 213.
  • Fig. 5 shows the discharge state in which the ejector 217 is moved down and the pipette tip 101 is completely moved past the O-ring seal 213.
  • the pipetting tube 205 of the pipetting unit 201 will be associated with suitable suction means, which make it possible to generate a negative pressure in the pipetting channel 207 and thus in the pipette tip 101, which leads to the aspiration of liquid to be pipetted.
  • suction means may comprise, for example, a piston which is axially movable in the pipetting tube 205 and which is axially adjustable by means of electrical, hydraulic or pneumatic actuating means.
  • the pipette tip 101 is preferably made of a plastic material, for example by injection molding.
  • This plastic material may be electrically conductive in order to be able to conduct conductance measurements on the liquid to be pipetted in a manner known per se.
  • the coupling sleeve 211 and the pipetting tube 205 may be made of conductive materials.
  • metals are preferably used here, although plastic materials for the coupling sleeve 211 and the pipetting tube 205 are not excluded.
  • the capacity of the disposable pipette tip 101 may be, for example, between 0.1 and 1300 ⁇ l.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (12)

  1. Unité formant pipette (201) pourvue d'une pointe de pipette destinée à un dispositif à pipetter, l'unité formant pipette (201) présentant une pièce rapportée d'accouplement (203), sur laquelle la pointe de pipette est susceptible d'être enfoncée, dans une direction d'enfoncement, pour être accouplée, la pièce rapportée d'accouplement (203) présentant un organe de précontrainte (213) déplaçable, susceptible de passer d'un état de précontrainte à un état libéré grâce à la présence d'un dispositif d'actionnement (215) prévu dans la pièce rapportée d'accouplement,
    caractérisée en ce que
    la pointe de pipette (101) présente une butée axiale (121) qui coopère, dans la position axiale d'accouplement de la pointe de pipette (101), avec une butée complémentaire (219) de la pièce rapportée d'accouplement (203), en ce que l'organe de précontrainte (213), dans son état de précontrainte, s'applique sur une surface d'application (127) de la pointe de pipette (101) enfoncée, par des composantes de surface de la pointe de pipette (101) qui sont orientées à l'opposé de la direction d'enfoncement, s'étendant radialement vers l'intérieur, de telle sorte qu'il repose de façon étanche contre la surface d'application et précontraigne la pointe de pipette (201) de l'unité formant pipette (201) dans la direction axiale d'accouplement, et en ce que l'organe de précontrainte (213), dans son état libéré, laisse sensiblement à découvert la surface d'application (127) de la pointe de pipette (101), la surface d'application (127) étant constituée par une rainure annulaire (125) incurvée en forme d'arc de cercle.
  2. Unité formant pipette selon la revendication 1, caractérisée en ce que la butée axiale (121) est située, au moins partiellement, sur une circonférence interne (117) d'une enveloppe (105) de la pointe de pipette, à distance axiale de l'extrémité (109) à laquelle la pointe de pipette (101) est enfoncée.
  3. Unité formant pipette selon la revendication 2, caractérisée en ce que la circonférence interne (117) de l'enveloppe possède, au moins dans la zone d'accouplement (111), une extrémité formant gaine constituée sensiblement par des tronçons cylindriques (119, 123).
  4. Unité formant pipette selon l'une des revendications précédentes, caractérisée en ce que la butée axiale (121) est constituée par un épaulement de la circonférence interne (117) de l'enveloppe qui relie un premier tronçon cylindrique de circonférence interne d'enveloppe (119), de plus gros diamètre, proche de l'extrémité (109) à laquelle la pointe de pipette est enfoncée, avec un deuxième tronçon cylindrique de circonférence interne d'enveloppe (123), de plus petit diamètre, éloigné de l'extrémité (109) à laquelle la pointe de pipette est enfoncée.
  5. Unité formant pipette selon la revendication 4, caractérisée en ce que la diminution de diamètre, entre le premier (119) et le deuxième (123) tronçon de circonférence interne d'enveloppe, due à la présence de l'épaulement (121), est comprise entre 0,8 et 1,2 mm.
  6. Unité formant pipette selon la revendication 4 ou 5, caractérisée en ce que le diamètre du premier tronçon de circonférence interne d'enveloppe (119) est compris entre 6,5 et 7,1 mm.
  7. Unité formant pipette selon l'une des revendications 4 à 6, caractérisée en ce que le diamètre du deuxième tronçon de circonférence interne d'enveloppe (123) est compris entre 5,5 et 6,1 mm.
  8. Unité formant pipette selon l'une des revendications précédentes, caractérisée en ce que la section transversale de la rainure annulaire (125) est délimitée par un arc de cercle et possède un rayon de courbure compris entre 0,3 et 0,9 mm.
  9. Unité formant pipette selon l'une des revendications précédentes, caractérisée en ce que la surface d'application (127) est située, axialement, entre la butée axiale (121) et l'extrémité (109) à laquelle la pointe de pipette (101) est enfoncée.
  10. Unité formant pipette selon l'une des revendications précédentes, caractérisée en ce que la butée complémentaire (219) est constituée par un épaulement présent sur la circonférence externe de la pièce rapportée d'accouplement (203).
  11. Unité formant pipette selon l'une des revendications précédentes, caractérisée en ce que la pièce rapportée d'accouplement (203) porte un élément d'étanchéité (213) servant à assurer l'étanchéité entre la pointe de pipette (101) et la pièce rapportée d'accouplement (203), constitué en matériau élastiquement déformable et, si cela est souhaité, en matériau conduisant l'électricité, et en ce qu'est associé, à l'unité formant pipette (201), un dispositif d'écrasement (215) destiné à comprimer axialement l'élément d'étanchéité (213).
  12. Unité formant pipette selon la revendication 11, caractérisée en ce que l'élément d'étanchéité (213) est un joint annulaire.
EP00926950A 1999-04-16 2000-04-14 Pointe de pipette, dispositif a pipetter et combinaison de pointe de pipette et de dispositif de pipette Expired - Lifetime EP1171240B2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19917375 1999-04-16
DE19917375A DE19917375C2 (de) 1999-04-16 1999-04-16 Pipettiereinheit
PCT/EP2000/003423 WO2000062933A1 (fr) 1999-04-16 2000-04-14 Pointe de pipette, dispositif a pipetter et combinaison de pointe de pipette et de dispositif de pipette

Publications (3)

Publication Number Publication Date
EP1171240A1 EP1171240A1 (fr) 2002-01-16
EP1171240B1 EP1171240B1 (fr) 2003-07-02
EP1171240B2 true EP1171240B2 (fr) 2006-06-14

Family

ID=7904895

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00926950A Expired - Lifetime EP1171240B2 (fr) 1999-04-16 2000-04-14 Pointe de pipette, dispositif a pipetter et combinaison de pointe de pipette et de dispositif de pipette

Country Status (7)

Country Link
US (2) US7033543B1 (fr)
EP (1) EP1171240B2 (fr)
JP (1) JP3977597B2 (fr)
AT (1) ATE244070T1 (fr)
DE (2) DE19917375C2 (fr)
ES (1) ES2197094T5 (fr)
WO (1) WO2000062933A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10493444B2 (en) 2015-10-13 2019-12-03 Roche Molecular Systems, Inc. Pipetting device for an apparatus for processing a sample or reagent, apparatus for processing a sample or reagent and method for pipetting a sample or reagent

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001275195A1 (en) * 2000-06-26 2002-01-08 Vistalab Technologies, Inc. Automatic pipette identification and detipping
US6596240B2 (en) * 2001-01-12 2003-07-22 Porex Corporation Pipette tip for easy mounting and ejecting from a pipette
US6973845B2 (en) * 2001-01-18 2005-12-13 Beckman Coulter, Inc. Low insertion force tip/mandrel
FI20010972A0 (fi) * 2001-05-09 2001-05-09 Thermo Labsystems Oy Kärkisäiliöpipetti
US6568288B2 (en) * 2001-06-29 2003-05-27 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft
US7335337B1 (en) * 2001-09-11 2008-02-26 Smith James C Ergonomic pipette tip and adapters
JP4558995B2 (ja) * 2001-09-12 2010-10-06 ベックマン コールター， インコーポレイテッド 移送ユニットおよびその移送ユニットを備える自動分析装置
EP1329262B1 (fr) 2002-01-16 2009-08-19 Brand Gmbh + Co Kg Pipette et procédé pour sa production
US20040141885A1 (en) * 2002-02-12 2004-07-22 Molecular Devices Corp. Pipettor systems and components
US6793891B2 (en) * 2002-04-08 2004-09-21 Felxi Yiu Pipettor and externally sealed pipette tip
US7517694B2 (en) * 2002-07-26 2009-04-14 Ortho-Clinical Diagnostics, Inc. Metering tip with internal features to control fluid meniscus and oscillation
DE10345324B3 (de) * 2003-09-30 2004-08-19 Eppendorf Ag Pipettenspitze
US7641859B2 (en) * 2004-02-11 2010-01-05 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip
CA2512353A1 (fr) * 2004-07-16 2006-01-16 Stemcell Technologies Inc. Machine a pipette automatisee
JP4224488B2 (ja) * 2005-11-14 2009-02-12 株式会社アイディエス 検体分取分注用ノズル装置
EP1862219B1 (fr) * 2006-05-29 2018-02-07 Qiagen GmbH Appareil pour retenir un embout à pipette et pipette utilisant ledit embout
DE102006036764B4 (de) * 2006-08-05 2012-11-29 Eppendorf Ag Pipettiersystem
US20080078258A1 (en) * 2006-09-28 2008-04-03 Price West L Multi-component pipette tip and associated methods
US7662343B2 (en) * 2006-10-24 2010-02-16 Viaflo Corporation Locking pipette tip and mounting shaft
US7662344B2 (en) 2006-10-24 2010-02-16 Viaflo Corporation Locking pipette tip and mounting shaft
US7794664B2 (en) 2006-11-16 2010-09-14 Idexx Laboratories, Inc. Pipette tip
WO2008068605A1 (fr) * 2006-12-07 2008-06-12 Gilson S.A.S. Dispositif de montage d'embout pour dispositif d'échantillonnage
JP4231522B2 (ja) * 2006-12-07 2009-03-04 株式会社アイディエス 回転式分取分注装置
US8273268B2 (en) 2007-08-13 2012-09-25 Polyone Corporation Electrically conductive polyolefin blends
US20090071267A1 (en) * 2007-09-17 2009-03-19 Greg Mathus Pipette tip ejection mechanism
EP2197584A4 (fr) * 2007-09-19 2011-08-03 Sorenson Bioscience Inc Support pour embouts de pipette et procédés associés
US20090181359A1 (en) * 2007-10-25 2009-07-16 Lou Sheng C Method of performing ultra-sensitive immunoassays
US8222048B2 (en) 2007-11-05 2012-07-17 Abbott Laboratories Automated analyzer for clinical laboratory
US8691149B2 (en) * 2007-11-06 2014-04-08 Abbott Laboratories System for automatically loading immunoassay analyzer
US8273009B2 (en) * 2008-03-07 2012-09-25 Cook Medical Technologies Llc Pipette aspiration device
US8202495B1 (en) * 2008-06-23 2012-06-19 Smith James C Ergonomic pipette tip
ES2567067T3 (es) 2008-11-28 2016-04-19 F. Hoffmann-La Roche Ag Sistema y método para la extracción automática de ácidos nucleicos
EP2191900B1 (fr) * 2008-11-28 2016-03-30 F. Hoffmann-La Roche AG Système et précédé pour le traitement d'un fluide contenant des acides nucléiques
US8318499B2 (en) 2009-06-17 2012-11-27 Abbott Laboratories System for managing inventories of reagents
US8715574B2 (en) 2009-06-19 2014-05-06 Abbott Laboratories System for managing inventory of bulk liquids
US8277757B2 (en) * 2009-09-29 2012-10-02 Integra Biosciences Corp. Pipette tip mounting shaft
US8211313B2 (en) 2009-12-21 2012-07-03 Abbott Laboratories System for processing magnetic particles
JP5635811B2 (ja) * 2010-05-28 2014-12-03 深江化成株式会社 ピペット装置
FI123648B (fi) * 2010-11-15 2013-08-30 Thermo Fisher Scientific Oy Monikanavapipetti
US8524170B2 (en) * 2011-02-22 2013-09-03 Rainin Instrument, Llc Pipette and sealing tip
DE102011006581A1 (de) 2011-03-31 2012-10-04 Hamilton Bonaduz Ag Zustandsüberwachung einer Pipettenspitze mit von innen angekoppelten piezoelektrischen Elementen
JP5740632B2 (ja) * 2011-05-25 2015-06-24 メディカテック株式会社 分注装置
EP2718017B1 (fr) * 2011-06-06 2020-08-05 Corsolutions, LLC Interface fluidique
PT106204A (pt) 2012-03-12 2013-09-12 Inst Politecnico De Leiria Ponta de pipetadora estanque para centrífuga
DE102012213089A1 (de) 2012-07-25 2014-01-30 Hamilton Bonaduz Ag Kopplungsausbildung eines Pipettierkanals einer Pipettiervorrichtung zur Ankopplung einer Pipettierspitze daran
FI125139B (en) 2012-08-20 2015-06-15 Thermo Fisher Scientific Oy Pipette and a method for mounting a pipette tip into a pipette
US10416046B2 (en) 2013-04-11 2019-09-17 Rarecyte, Inc. Device, system, and method for selecting a target analyte
US10088392B2 (en) * 2013-04-11 2018-10-02 Rarecyte, Inc. Device, system, and method for selecting a target analyte or fluid
US10285564B2 (en) 2013-04-30 2019-05-14 Grenova, Inc. Pipette tip washing device
CN105209186B (zh) 2013-04-30 2017-10-27 格瑞诺瓦有限责任公司 移液器吸头清洗装置
US9744570B2 (en) 2013-04-30 2017-08-29 Grenova, Llc Pipette tip washing device
DE102013218002A1 (de) * 2013-09-09 2015-03-12 Hamilton Bonaduz Ag Pipettiervorrichtung mit einer Kopplungsformation zur Ankopplung einer gesondert ausgebildeten Pipettierspitze und mit einem durch Magnetfeld formveränderbarem Verriegelungskörper zur Verriegelung und Entriegelung der Pipettierspitze an der Kopplungsformation
CH709307A1 (de) * 2014-02-26 2015-08-28 Tecan Trading Ag Transportwerkzeug zum Transportieren eines Laborartikels.
JP6260379B2 (ja) * 2014-03-18 2018-01-17 株式会社島津製作所 ガス吹付式液体注入装置及びそれに用いられる注入容器
DE102014108688A1 (de) 2014-06-20 2015-12-24 Hamilton Bonaduz Ag Pipettiervorrichtung mit modularem Pipettierkopf
DE102015213005A1 (de) * 2015-07-10 2017-01-12 Hamilton Bonaduz Ag Pipettiervorrichtung mit ausstülpbarer Kopplungsformation zur Ankopplung einer Pipettierspitze sowie hierfür ausgebildete Pipettierspitze
WO2017064088A1 (fr) 2015-10-13 2017-04-20 F. Hoffmann-La Roche Ag Dispositif de pipetage pour appareil de traitement d'échantillon ou réactif, appareil de traitement d'un échantillon ou réactif et procédé permettant de pipeter un échantillon ou réactif
WO2017139262A1 (fr) * 2016-02-12 2017-08-17 Grenova, Llc Dispositif de lavage d'embout de pipette
CA3014617C (fr) 2016-02-17 2023-08-22 Becton, Dickinson And Company Systeme de preparation d'echantillons automatise a des fins de tests diagnostiques
CN207164073U (zh) 2016-04-22 2018-03-30 贝克顿·迪金森公司 自动化诊断分析仪
EP4234182A3 (fr) 2016-04-22 2024-03-20 Becton, Dickinson and Company Analyseur automatisé perforant le bouchon pour aspirer
US11065614B2 (en) 2016-06-15 2021-07-20 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11235318B2 (en) 2016-06-15 2022-02-01 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
CN109562372A (zh) * 2016-06-15 2019-04-02 汉密尔顿公司 移液装置、移液管末梢联接器和移液管末梢：装置和方法
US10898892B2 (en) 2016-06-15 2021-01-26 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
DE102016111910A1 (de) 2016-06-29 2018-01-04 Eppendorf Ag Dosierkopf, Dosiervorrichtung umfassend einen Dosierkopf und Verfahren zum Dosieren mittels eines Dosierkopfes
DE102016111912A1 (de) 2016-06-29 2018-01-04 Eppendorf Ag Dosierkopf, Dosiervorrichtung umfassend einen Dosierkopf und Verfahren zum Dosieren mittels eines Dosierkopfes
US11167282B2 (en) 2017-01-31 2021-11-09 HighRes Biosolutions, Inc. Auto-pipetting apparatus and method
DE202017101007U1 (de) * 2017-02-23 2018-05-24 Brand Gmbh + Co Kg Austauschbare Kolben-Zylinder-Einheit für einen Dispenser, Dispenser und System zum Aufnehmen und Abgeben von Fluidvolumina
JP7089299B2 (ja) 2017-07-21 2022-06-22 シージーン アイエヌシー 磁性モジュール及びカバーモジュールを用いた核酸抽出方法、磁性モジュール、カバーモジュール、部品、並びに自動化システム
DE102017220042A1 (de) 2017-11-10 2019-05-16 Hamilton Bonaduz Ag Pipettiervorrichtung mit elektromagnetisch antreibbarem Verriegelungsaktuator zur Verriegelung von lösbar angekuppelten Pipettierspitzen
EP3666379A1 (fr) 2018-12-14 2020-06-17 Eppendorf AG Tête de pipettage, dispositif de pipettage comprenant une tête de pipettage et procédé de pipettage à l'aide d'une tête de pipettage
PL3680016T3 (pl) * 2019-01-08 2022-04-04 Eppendorf Ag Pipeta do używania z końcówką
PL3680017T3 (pl) * 2019-01-08 2022-02-07 Eppendorf Ag Pipeta do używania z końcówką
JP7404923B2 (ja) * 2019-04-02 2023-12-26 東ソー株式会社 分注装置
JP7367596B2 (ja) * 2019-11-26 2023-10-24 横河電機株式会社 チップデバイス、チップ容器セット、チップ電極装置及びチップ電極セット
EP4067471A4 (fr) * 2019-11-26 2024-06-26 Yokogawa Electric Corporation Dispositif de pointe, ensemble contenant de pointe, dispositif d'électrode de pointe et ensemble d'électrodes de pointe
WO2021146126A1 (fr) * 2020-01-15 2021-07-22 Perkinelmer Health Sciences, Inc. Pointes de pipette et procédés et systèmes les comprenant
CN115803115A (zh) * 2020-07-13 2023-03-14 安捷伦科技有限公司 移液管吸头装置和方法
WO2023088850A1 (fr) * 2021-11-16 2023-05-25 Ritter Gmbh Pointe de pipette, et procédé de production de la pointe de pipette
DE102022120212A1 (de) 2022-08-10 2024-02-15 Hamilton Bonaduz Ag Pipettierspitze mit sich gekrümmt verjüngendem Aufnahmeraum

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB272218A (en) 1926-06-02 1927-10-13 British Thomson Houston Co Ltd Improvements in and relating to electric arc welding
US3732734A (en) * 1972-05-25 1973-05-15 Centaur Chemical Co Micropipette with disposable tips
DE7518809U (de) * 1975-06-12 1978-09-14 Eppendorf Geraetebau Netheler & Hinz Gmbh, 2000 Hamburg Pipettenspitze
US4187724A (en) * 1978-01-11 1980-02-12 Indicon Inc. Replaceable tip for a pipette
FI843976L (fi) * 1983-10-13 1985-04-14 Cetus Corp Utbytbar pipettspets.
GB2172218B (en) * 1985-02-15 1988-07-20 Rocket Of London Ltd Pipetter tip for pipetters
US4679446A (en) * 1985-09-09 1987-07-14 Baxter Travenol Laboratories, Inc. Multi-volume displacement pipette
US4824641A (en) * 1986-06-20 1989-04-25 Cetus Corporation Carousel and tip
US4748859A (en) * 1987-03-06 1988-06-07 Rainin Instrument Co., Inc. Disposable pipette tip
US4863695A (en) * 1987-04-28 1989-09-05 Hewlett-Packard Company Pipette assembly
US5306510A (en) * 1988-01-14 1994-04-26 Cyberlab, Inc. Automated pipetting system
GB8808583D0 (en) * 1988-04-12 1988-05-11 Flow Lab Pipette tip pickup apparatus
DE3824767A1 (de) * 1988-07-21 1990-02-01 Eppendorf Geraetebau Netheler Aufsteckbare pipettenspitze in form eines entsprechend einem aufsteckkopfstueck, insbesondere konus einer pipette, wenigstens abschnittsweise ausgefuehrten gefaesses
JP2701900B2 (ja) * 1988-12-20 1998-01-21 株式会社ニチリョー マルチピペット
US5200151A (en) * 1990-05-21 1993-04-06 P B Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
US5525302A (en) * 1991-02-01 1996-06-11 Astle; Thomas W. Method and device for simultaneously transferring plural samples
US5223225A (en) * 1991-05-17 1993-06-29 Bio 101 Scale-marked pipet tip for precision dispensing of fluids over a large range of volumes
US5232669A (en) * 1991-11-08 1993-08-03 Abbott Laboratories Pipette tip with self-aligning and self-sealing features
FI925118A0 (fi) * 1992-11-11 1992-11-11 Labsystems Oy Behaollare
US5827745A (en) * 1993-03-29 1998-10-27 Astle; Thomas W. Micropipette tip loading and unloading device and method and tip package
JP3395250B2 (ja) 1993-05-10 2003-04-07 ソニー株式会社 ピペット装置
CA2147560A1 (fr) * 1994-04-22 1995-10-23 Donald H. Devaughn Appareil avec haut de pipette resistant au transfert de liquide et d'aerosol; methode de reduction de transfert
US5496523A (en) * 1994-05-06 1996-03-05 Sorenson Bioscience Filtered micropipette tip for high/low volume pipettors
JP3387649B2 (ja) * 1994-09-16 2003-03-17 富士写真フイルム株式会社 点着チップ
JP3630493B2 (ja) * 1995-03-20 2005-03-16 プレシジョン・システム・サイエンス株式会社 分注機を利用した液体処理方法およびその装置
EP0733404A1 (fr) * 1995-03-24 1996-09-25 Becton, Dickinson and Company Embout de pipette
JPH09127130A (ja) * 1995-08-31 1997-05-16 Teruaki Ito 検体分取分注用ノズル装置
US20010036425A1 (en) * 1995-10-12 2001-11-01 Michel Gazeau Device for transferring samples of micro-amounts of liquids
US5807524A (en) * 1996-08-06 1998-09-15 Rainin Instrument Co., Inc. Pipette tip with pipette surface contamination protector
US6066297A (en) * 1997-01-03 2000-05-23 Matrix Technologies Corporation Small sample volume displacement pipette tips
US6123905A (en) * 1997-01-17 2000-09-26 Matrix Technologies Corporation Pipettor including an indicator and method of use
US5948359A (en) * 1997-03-21 1999-09-07 Biogenex Laboratories Automated staining apparatus
US5851491A (en) 1997-06-13 1998-12-22 Labcon, North America Pipette tip and filter for accurate sampling and prevention of contamination
US6499363B1 (en) * 1997-12-12 2002-12-31 The Sailor Pen Co., Ltd. Tip for pipette and pipette with the same
US6495106B1 (en) * 1998-03-24 2002-12-17 Biogenex Laboratories Automated staining apparatus
US6106783A (en) * 1998-06-30 2000-08-22 Microliter Analytical Supplies, Inc. Microplate assembly and closure
US6197259B1 (en) * 1998-11-06 2001-03-06 Rainin Instrument Co., Inc. Easy eject pipette tip
US6168761B1 (en) * 1998-11-06 2001-01-02 Rainin Instrument Co., Inc. Pipette with improved pipette tip and mounting shaft
US6248295B1 (en) * 1998-11-06 2001-06-19 Rainin Instrument Co., Inc. Pipette with improved pipette tip and mounting shaft combination
US6723237B1 (en) * 1999-01-18 2004-04-20 Precision Systems Science Co., Ltd. Concentration device using magnetic particles and method therefor
US6673318B1 (en) * 1999-05-31 2004-01-06 Bridgestone Corporation Pipette
US6399024B1 (en) * 2000-02-01 2002-06-04 Incyte Genomics, Inc. Multichannel pipette head
US6532837B1 (en) * 2000-02-03 2003-03-18 Rainin Instrument, Llc Pipette device with tip ejector utilizing stored energy
US6566145B2 (en) * 2000-02-09 2003-05-20 William E Brewer Disposable pipette extraction
DE10013511A1 (de) * 2000-03-20 2001-10-11 Brand Gmbh & Co Kg Mehrkanal-Pipettiereinrichtung sowie Pipettenschaft dafür
AU2001275195A1 (en) * 2000-06-26 2002-01-08 Vistalab Technologies, Inc. Automatic pipette identification and detipping
JP4271835B2 (ja) * 2000-08-18 2009-06-03 アークレイ株式会社 ピペット装置
FI20002761A0 (fi) * 2000-12-15 2000-12-15 Wallac Oy Monikanavainen pipetointilaitteisto
US6596240B2 (en) * 2001-01-12 2003-07-22 Porex Corporation Pipette tip for easy mounting and ejecting from a pipette
FI20010972A0 (fi) * 2001-05-09 2001-05-09 Thermo Labsystems Oy Kärkisäiliöpipetti
US7335337B1 (en) * 2001-09-11 2008-02-26 Smith James C Ergonomic pipette tip and adapters
US6967004B2 (en) * 2001-10-30 2005-11-22 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft
DE10222511B4 (de) * 2002-05-22 2005-03-17 Eppendorf Ag Pipettenspitze
US20040071602A1 (en) * 2002-10-15 2004-04-15 Yiu Felix H. Pipettor head adapter
US20050089450A1 (en) * 2003-10-27 2005-04-28 Integrated Instrument Services, Inc. Pipette with multiple sealing zones
US7641859B2 (en) * 2004-02-11 2010-01-05 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10493444B2 (en) 2015-10-13 2019-12-03 Roche Molecular Systems, Inc. Pipetting device for an apparatus for processing a sample or reagent, apparatus for processing a sample or reagent and method for pipetting a sample or reagent

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DE50002736D1 (de) 2003-08-07
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US7033543B1 (en) 2006-04-25
WO2000062933A1 (fr) 2000-10-26
US20060233669A1 (en) 2006-10-19
DE19917375A1 (de) 2000-11-02
US9415388B2 (en) 2016-08-16
DE19917375C2 (de) 2001-09-27
ATE244070T1 (de) 2003-07-15
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ES2197094T5 (es) 2006-11-16
JP3977597B2 (ja) 2007-09-19

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