EP0733404A1 - Embout de pipette - Google Patents

Embout de pipette Download PDF

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Publication number
EP0733404A1
EP0733404A1 EP96301946A EP96301946A EP0733404A1 EP 0733404 A1 EP0733404 A1 EP 0733404A1 EP 96301946 A EP96301946 A EP 96301946A EP 96301946 A EP96301946 A EP 96301946A EP 0733404 A1 EP0733404 A1 EP 0733404A1
Authority
EP
European Patent Office
Prior art keywords
pipette tip
foam
fluid
distal end
barrier
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96301946A
Other languages
German (de)
English (en)
Inventor
Michael L. Lamos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of EP0733404A1 publication Critical patent/EP0733404A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips

Definitions

  • the present invention relates to an aerosol barrier for use in a disposable pipette tip and, more particularly, to a disposable pipette tip having an aerosol barrier made from an absorbent open cell foam that provides sufficient air flow through the disposable pipette tip to perform air flow fluid detection but sufficiently blocks droplets and aerosol from flowing through the disposable pipette tip.
  • a pipette is a device commonly used in reagent laboratories as a fluid transfer device to perform addition and mixing of liquid components, i.e., reagents and samples.
  • disposable pipette tips were introduced to help laboratories avoid the problems of cross-contamination associated with reusing pipettes.
  • the disposable pipette tips were attached to the distal end of mechanical aspirators, i.e., a pipettor, and were designed to fit on the end of the mechanical aspirator so that the only air path from the mechanical aspirator was out the distal end of the disposable pipette tip.
  • the mechanical aspirator typically used a plunger to push air through the aspirator and out the distal end of the disposable pipette tip, prior to the distal end being inserted into a fluid to be aspirated from a source container.
  • the plunger on the mechanical aspirator was released to draw a vacuum into the disposable pipette tip and thereby pull fluid from the source container into the disposable pipette tip.
  • the aspirated fluid contained in the disposable pipette tip could then be dispensed into a target container using the plunger.
  • disposable pipette tips were commonly ejected from the mechanical aspirator using a tubular or ejector sleeve that pressed against the top of the disposable pipette tip to push the pipette tip off the mechanical aspirator.
  • the operation of ejecting the disposable pipette tip from the mechanical aspirator in this manner has the potential for creating aerosols from liquid residues within the disposable tip within the mechanical aspirator.
  • aerosols within the mechanical aspirator can also be generated when aspirating fluid into the disposable pipette tip. Aerosols coming from the pipette tip into the mechanical aspirator can potentially contaminate the mechanical aspirator and, therefore, other reagents or samples.
  • One proposed solution to the aerosol problem was to add a porous barrier between the fluid and the mechanical aspirator.
  • a hydrophobic filter plug made of polyethylene, for example, has been used to prevent fluid and aerosol passage from the distal end of the pipette tip into the mechanical aspirator without impacting aspirations and dispensing of the fluid from the disposable pipette tip.
  • Robotic aspirating instruments have come to replace manual mechanical aspirators, especially in high throughput, or more labor intensive environments that require more automation.
  • Robotic aspirating instruments have typically consisted of a stainless steel needle that aspirates fluid into its distal end from a target container by applying a vacuum to its proximal end by hydraulic displacement, for example.
  • robotic aspirating instruments must be washed periodically to prevent cross-contamination of reagents or samples.
  • disposable pipette tips have been attached to the robotic aspirating instruments to reduce the risk of cross-contamination.
  • the controller of the instrument it is critical for the controller of the instrument to detect when its distal end enters the fluid in the target container.
  • Aerosol filter barriers used in disposable pipette tips are often submicron hydrophobic plugs that cause large resistance to air flow. Such barriers are too dense to allow sufficient air flow, which causes a detrimental effect on small volume pipetting and does not work at all with automated robotic aspirating instruments that use air flow fluid detection. Therefore, there is the need for a new kind of barrier to be used in disposable pipette tips that will sufficiently entrap fluid and aerosol from flowing into the robotic aspirating instrument, but provide sufficient air flow for an air flow fluid detection system to operate.
  • the present invention overcomes the problems identified in the background material by providing a barrier for a disposable pipette tip that allows sufficient air flow through the pipette tip into a robotic aspirating instrument for an air flow detection system to operate, but continues to sufficiently entrap fluid and aerosol from flowing through the pipette tip and into the robotic aspirating instrument.
  • a preferred embodiment of a pipette tip according to the present invention includes a cannula having a distal and proximal end, with the proximal end being larger than the distal end and both ends being connected by a passageway.
  • a barrier is mounted within the passageway of the pipette tip near the cannula's proximal end to capture and prevent fluid and aerosol from flowing through the pipette tip and out the proximal end.
  • the barrier allows sufficient air flow through the pipette tip to permit air flow liquid detection systems in a robotic aspirating instrument to properly operate.
  • the barrier is made from a highly absorbent open cell foam that can sufficiently trap aerosols but not restrict air flow.
  • Fig. 1 is a perspective view of a pipette tip 10, according to the present invention, for use on a manual or robotic aspirating instrument to perform fluid transfer in a laboratory.
  • Pipette tip 10 includes an open cannula 15 having a generally tapering conical shape defined by a cylindrical outer wall 17.
  • Cannula 15 includes an open proximal end 20 and an open distal end 25 with a plurality of optional annular gradations 130, 135 and 140 on outer wall 17 therebetween.
  • Cannula 15 also includes a plurality of ribs 45 projecting from outer wall 17 and extending longitudinally from proximal end 20 towards distal end 25. Ribs 45 are provided to vertically support pipette tip 10, when attaching pipette tip 10 to an aspirating instrument. Vertical axial alignment is critical to robotic attachment of pipette tip 10 to an aspirating instrument, especially if automatic vision aids are not present.
  • Optional gradations 130, 135 and 140 divide cannula 15 into sections 30, 35 and 40 with each gradation 130, 135 and 140 representing a predetermined volume of liquid that may be located between the respective gradation and distal end 25 within cannula 15. These gradations are used by laboratory workers when visually measuring the volume of aspirated liquid during a manual pipetting operation.
  • the approximate volume of liquid contained in section 30 of cannula 15 between distal end 25 and gradation 130 is 10 ⁇ l; when the level is aligned with gradation 135 the approximate volume of liquid in sections 30 and 35 is 50 ⁇ l; and when the level is aligned with gradation 140 the approximate volume of liquid in sections 30, 35 and 40 is 100 ⁇ l.
  • the total volume of liquid that can be held in this usable portion of pipette tip 10 is approximately 120 ⁇ l.
  • the above-noted volumes are merely exemplary, the present invention will also work and be very beneficial for pipette tips having larger and smaller volumes with or without gradations.
  • Fig. 2 is a cross-sectional view of pipette tip 10, shown in Fig. 1 along lines 2-2, and shows a passageway 50 with a generally tapering conical shape extending from proximal end 20 to distal end 25.
  • passageway 50 is defined by an interior wall 57 having a diameter that decreases from proximal end 20 to distal end 25.
  • a foam barrier 60 is contained within passageway 50 near proximal end 20 and is preferably mounted in place using a light friction press fit.
  • other means i.e., adhesive, could also be used to mount foam barrier 60 in position.
  • Foam barrier 60 is preferably an open cell aerosol absorbing foam having a web-like or filamentous structure, similar to that shown in Fig. 3, that permits the flow of air through the foam but prevents droplets or aerosols from passing through the foam.
  • Fig. 3 is an electron microscope photograph of an actual foam barrier 60 for use in pipette tip 10, according to the present invention.
  • Foam barrier 60 having the structure of the present invention properly performs as a barrier to fluid droplets and aerosol that attempt to travel up passageway 50 from distal end 25 towards proximal end 20, during an aspiration process or when pipette tip 10 is ejected from the distal end of the aspirating instrument, and does not overly restrict the air flow necessary to perform automated air flow fluid detection.
  • pipette tip 10 containing foam barrier 60 is attached to the distal end of the aspirating instrument and held in place by friction on the distal end of the aspirating instrument.
  • the distal end of the aspirating instrument with pipette tip 10 thereon is moved over the source container and slowly lowered into the fluid to be aspirated.
  • the aspirating instrument generates an air flow through pipette tip 10 which is monitored by a controller to detect a change in air flow, when distal end 25 of pipette tip 10 enters the fluid.
  • the aspirating instrument can determine that pipette tip 10 has entered the fluid and can begin an aspiration process, wherein a vacuum is applied to proximal end 20 to aspirate fluid through distal end 25 of pipette tip 10 and into passageway 50. The vacuum is applied until a predetermined volume of fluid has been aspirated into passageway 50. Then, the aspirating instrument extracts pipette tip 10 from the fluid source and moves pipette tip 10 to the target container to dispense the aspirated fluid.
  • the aspirating instrument ejects pipette tip 10 from its distal end into a disposal container which violently displaces a large volume of air from proximal end 20 of passageway 50 in pipette tip 10 causing a momentary vacuum, and high velocity aerosolization of the residual fluid volume contained within distal end 25 of pipette tip 10.
  • foam barrier 60 is designed and formed of a material, described below, that captures any such fluid and aerosol to prevent it from flowing into, impacting upon, or being passed by the aspirating instrument.
  • Cannula 15 can be made from any form of plastic and foam barrier 60 can be made from polyurethane, polyvinylchloride or polyvinylalcohol.
  • foam barrier 60 is formed from a melamine-based open cell foam with a density in range of 0.5 to 0.8 pcf, with the most preferred density being 0.7 pcf.
  • Foams having these general properties are available from a number of suppliers for use as a thermal and acoustical insulation.
  • WilltecTM foam available from illbruck, Inc. in Minneapolis, Minnesota is a flexible, lightweight melamine-based open cell foam having the preferred density of 0.7 pcf.
  • WilltecTM foam exhibits chemical resistance to organic solvents as well as to a series of diluted acids and alkali.
  • Other physical properties of WilltecTM foam are: Physical Properties Typical Values Test Methods Tensile Strength 8.0 psi ASTM D3574-77 Tear Strength 0.3 lb/in ASTM D3574-77 Ultimate Elongation 8.0% ASTM D3574-77 Temperature Stability -60°C to 150°C permanent 24 hours to 250°C possible
  • WilltecTM foam is, of course, merely exemplary of a foam that can be used to form a foam barrier 60 according to the present invention. More importantly, as described above, foam barrier 60 must be made from a foam material that does not overly restrict the flow of air through foam barrier 60, but does prevent droplets or aerosols from passing through foam barrier 60. It has been determined that an open cell foam structure, similar to that shown in Fig. 3, satisfies this criteria and that a closed, ball-like or globular structure, similar to that shown in Fig. 4 does not perform as well.
  • Fig. 4 is an electron microscope photograph of a prior art barrier found in conventional aerosol filtered pipette tips. The barrier shown in Fig.
  • foam barrier 60 from WilltecTM foam.
  • a cork borer is used to punch a "plug" shaped foam barrier 60 from a 0.25 inch thick sheet of WilltecTM foam.
  • the "plug” shaped foam barrier 60 is then is inserted through proximal end 20 of pipette tip 10 and pressed to a predetermined position within passageway 50.
  • this manufacturing technique and the materials and tool used are merely exemplary, various other manufacturing methods and materials could also be used.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
EP96301946A 1995-03-24 1996-03-21 Embout de pipette Withdrawn EP0733404A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41024595A 1995-03-24 1995-03-24
US410245 1999-09-30

Publications (1)

Publication Number Publication Date
EP0733404A1 true EP0733404A1 (fr) 1996-09-25

Family

ID=23623884

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96301946A Withdrawn EP0733404A1 (fr) 1995-03-24 1996-03-21 Embout de pipette

Country Status (2)

Country Link
EP (1) EP0733404A1 (fr)
JP (1) JP2803809B2 (fr)

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999046046A1 (fr) * 1998-03-09 1999-09-16 Biohit Oyj Dispositif d'aspiration
DE19917375A1 (de) * 1999-04-16 2000-11-02 Hamilton Bonaduz Ag Bonaduz Pipettenspitze, Pipettiervorrichtung und Kombination aus Pipettenspitze und Pipettiervorrichtung
US6596240B2 (en) 2001-01-12 2003-07-22 Porex Corporation Pipette tip for easy mounting and ejecting from a pipette
DE10345324B3 (de) * 2003-09-30 2004-08-19 Eppendorf Ag Pipettenspitze
EP1685901A1 (fr) * 2005-01-28 2006-08-02 Molecular BioProducts, Inc. Préèvement de liquide en utilisant un embout de pipette en nervures pour la pénétration d' une barrière
EP1685902A1 (fr) * 2005-01-28 2006-08-02 Molecular BioProducts, Inc. Prélèvement de liquide en utilisant un embout de pipette en nervures pour la pénétration d'une barrière
WO2007118803A1 (fr) * 2006-04-18 2007-10-25 Basf Se Mousse à base d'aminoplaste comme outil de travail pouvant être stérilisé
DE102006036764A1 (de) * 2006-08-05 2008-02-07 Eppendorf Ag Pipettiersystem, Pipettiervorrichtung und Pipettenspitze
US7641859B2 (en) 2004-02-11 2010-01-05 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip
CN102183671A (zh) * 2011-03-04 2011-09-14 苏州康容生物医疗科技有限公司 加长型导电一次性加样尖
CN102183672A (zh) * 2011-03-04 2011-09-14 苏州康容生物医疗科技有限公司 两段式导电一次性加样尖
DE102010031240A1 (de) * 2010-07-12 2012-01-12 Hamilton Bonaduz Ag Pipettierspitze mit hydrophober Oberflächenausbildung
CN105792939A (zh) * 2014-06-27 2016-07-20 株式会社爱安德 吸量管吸头
US20180299476A1 (en) * 2017-04-12 2018-10-18 Bio-Rad Laboratories, Inc. Liquid dispenser and method of use
US20180299475A1 (en) * 2017-04-12 2018-10-18 Bio-Rad Laboratories, Inc. Liquid dispenser
EP3471878A4 (fr) * 2016-06-15 2019-11-13 Hamilton Company Dispositif de pipettage, coupleur de pointe de pipette, et pointe de pipette : dispositifs et procédés
US10898892B2 (en) 2016-06-15 2021-01-26 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11065614B2 (en) 2016-06-15 2021-07-20 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11235318B2 (en) 2016-06-15 2022-02-01 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10263421A (ja) * 1997-03-21 1998-10-06 Fukae Kasei Kk 粉体分与用ディスポーザブルピペットチップと装置
JP4346754B2 (ja) * 1999-10-26 2009-10-21 日東電工株式会社 ピペット用フィルターの製作方法
JP4447925B2 (ja) * 2002-04-08 2010-04-07 イユ、フェリックス・エイチ 改良されたピペッタ及び外部にシールが設けられたピペット
JP2005069830A (ja) * 2003-08-22 2005-03-17 Sysmex Corp 分注チップとそれを用いた分析装置
JP2005249535A (ja) * 2004-03-03 2005-09-15 Olympus Corp 分注プローブ及びこれを備えた自動分析装置
DE102005005437A1 (de) * 2005-02-05 2006-08-10 Eppendorf Ag Filterpipettenspitze
KR101348678B1 (ko) * 2007-05-23 2014-01-10 포항공과대학교 산학협력단 의료용 시험장치 및 이를 이용한 의료용 자동진단장치
US8307721B2 (en) 2009-01-12 2012-11-13 Biotix, Inc. Flexible pipette tips
US9486803B2 (en) 2010-01-22 2016-11-08 Biotix, Inc. Pipette tips
US8795606B2 (en) 2012-05-30 2014-08-05 Biotix, Inc. Integrated pipette tip devices
EP3183061B1 (fr) * 2014-08-20 2018-12-05 Corning Incorporated Embouts de pipette ayant des attributs améliorés et procédés de fabrication
US9927452B2 (en) * 2014-08-20 2018-03-27 Rai Strategic Holdings, Inc. Pipetting system
KR101785441B1 (ko) * 2015-03-16 2017-10-16 주식회사 르메디 개량된 피펫 팁
EP3492926B1 (fr) * 2016-07-26 2021-06-23 Konica Minolta, Inc. Embout de pipette, procédé de distribution de liquide et système de distribution de liquide
WO2018213196A1 (fr) 2017-05-17 2018-11-22 Biotix, Inc. Embouts de pipette ergonomiques
EP3717127A1 (fr) * 2017-11-30 2020-10-07 Corning Incorporated Pipette avec filtre encapsulé ou intégré, et procédé et appareil pour leur formation
EP3575000A1 (fr) * 2018-05-31 2019-12-04 Eppendorf AG Pointe de pipette
JP7388179B2 (ja) * 2019-12-24 2023-11-29 東ソー株式会社 鍔を設けた分注チップ

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DE2155566A1 (de) * 1971-11-09 1973-05-17 Helmut Dr Med Jansen Pipettierhilfe
US4119403A (en) * 1977-06-15 1978-10-10 W. R. Grace & Co. Method and apparatus for temperature controlled samplers
US4267729A (en) * 1979-05-14 1981-05-19 Eddelman Roy T Volumetric pipettor
US5156811A (en) * 1990-11-07 1992-10-20 Continental Laboratory Products, Inc. Pipette device
US5364595A (en) * 1993-07-02 1994-11-15 Porex Technologies Corp. Pipette device constructed to prevent contamination by aerosols or overpipetting
US5477256A (en) * 1992-03-27 1995-12-19 Scitex Digital Printing, Inc. Ink mist filter

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JP2536217Y2 (ja) * 1990-06-12 1997-05-21 住友ベークライト株式会社 ディスペンサー用チップ
JPH0539642U (ja) * 1991-10-31 1993-05-28 住友ベークライト株式会社 デイスペンサー用チツプ

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2155566A1 (de) * 1971-11-09 1973-05-17 Helmut Dr Med Jansen Pipettierhilfe
US4119403A (en) * 1977-06-15 1978-10-10 W. R. Grace & Co. Method and apparatus for temperature controlled samplers
US4267729A (en) * 1979-05-14 1981-05-19 Eddelman Roy T Volumetric pipettor
US5156811A (en) * 1990-11-07 1992-10-20 Continental Laboratory Products, Inc. Pipette device
US5477256A (en) * 1992-03-27 1995-12-19 Scitex Digital Printing, Inc. Ink mist filter
US5364595A (en) * 1993-07-02 1994-11-15 Porex Technologies Corp. Pipette device constructed to prevent contamination by aerosols or overpipetting

Cited By (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999046046A1 (fr) * 1998-03-09 1999-09-16 Biohit Oyj Dispositif d'aspiration
DE19917375A1 (de) * 1999-04-16 2000-11-02 Hamilton Bonaduz Ag Bonaduz Pipettenspitze, Pipettiervorrichtung und Kombination aus Pipettenspitze und Pipettiervorrichtung
DE19917375C2 (de) * 1999-04-16 2001-09-27 Hamilton Bonaduz Ag Bonaduz Pipettiereinheit
US9415388B2 (en) 1999-04-16 2016-08-16 Hamilton Bonaduz Ag Pipette tip, pipette device, and combination of pipette tip and pipette device
US7033543B1 (en) 1999-04-16 2006-04-25 Hamilton Bonaduz Ag Pipette tip, pipetting device and combination consisting of a pipette tip and pipetting device
US6596240B2 (en) 2001-01-12 2003-07-22 Porex Corporation Pipette tip for easy mounting and ejecting from a pipette
US7585467B2 (en) 2003-09-30 2009-09-08 Eppendorf Ag Pipette tip
DE10345324B3 (de) * 2003-09-30 2004-08-19 Eppendorf Ag Pipettenspitze
US8163256B2 (en) 2004-02-11 2012-04-24 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip
US7641859B2 (en) 2004-02-11 2010-01-05 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip
EP1685901A1 (fr) * 2005-01-28 2006-08-02 Molecular BioProducts, Inc. Préèvement de liquide en utilisant un embout de pipette en nervures pour la pénétration d' une barrière
EP1685902A1 (fr) * 2005-01-28 2006-08-02 Molecular BioProducts, Inc. Prélèvement de liquide en utilisant un embout de pipette en nervures pour la pénétration d'une barrière
WO2007118803A1 (fr) * 2006-04-18 2007-10-25 Basf Se Mousse à base d'aminoplaste comme outil de travail pouvant être stérilisé
DE102006036764B4 (de) * 2006-08-05 2012-11-29 Eppendorf Ag Pipettiersystem
DE102006036764A1 (de) * 2006-08-05 2008-02-07 Eppendorf Ag Pipettiersystem, Pipettiervorrichtung und Pipettenspitze
DE102010031240A1 (de) * 2010-07-12 2012-01-12 Hamilton Bonaduz Ag Pipettierspitze mit hydrophober Oberflächenausbildung
US8753715B2 (en) 2010-07-12 2014-06-17 Hamilton Bonaduz Ag Process for hydrophobically coating a pipette tip
US8840957B2 (en) 2010-07-12 2014-09-23 Hamilton Bonaduz Ag Process for hydrophobically coating a pipette tip
CN102183672A (zh) * 2011-03-04 2011-09-14 苏州康容生物医疗科技有限公司 两段式导电一次性加样尖
CN102183671A (zh) * 2011-03-04 2011-09-14 苏州康容生物医疗科技有限公司 加长型导电一次性加样尖
CN105792939A (zh) * 2014-06-27 2016-07-20 株式会社爱安德 吸量管吸头
EP3162442A4 (fr) * 2014-06-27 2018-01-03 A & D Company, Limited Embout de pipette
US10888858B2 (en) 2016-06-15 2021-01-12 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11065614B2 (en) 2016-06-15 2021-07-20 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
EP3471878A4 (fr) * 2016-06-15 2019-11-13 Hamilton Company Dispositif de pipettage, coupleur de pointe de pipette, et pointe de pipette : dispositifs et procédés
EP3578260A1 (fr) * 2016-06-15 2019-12-11 Hamilton Company Dispositif de pipetage, coupleur de pointe de pipette et pointe de pipette dispositifs et procédés
EP3599022A1 (fr) * 2016-06-15 2020-01-29 Hamilton Company Dispositif de pipetage, coupleur de pointe de pipette et pointe de pipette : dispositifs et procédés
US10661269B2 (en) 2016-06-15 2020-05-26 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US10682642B2 (en) 2016-06-15 2020-06-16 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US10730040B2 (en) 2016-06-15 2020-08-04 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US10766035B1 (en) 2016-06-15 2020-09-08 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US12005434B2 (en) 2016-06-15 2024-06-11 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US10898892B2 (en) 2016-06-15 2021-01-26 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11020738B2 (en) 2016-06-15 2021-06-01 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11065613B2 (en) 2016-06-15 2021-07-20 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11235318B2 (en) 2016-06-15 2022-02-01 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11117125B2 (en) 2016-06-15 2021-09-14 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US11130123B2 (en) 2016-06-15 2021-09-28 Hamilton Company Pipetting device, pipette tip coupler, and pipette tip: devices and methods
US20180299475A1 (en) * 2017-04-12 2018-10-18 Bio-Rad Laboratories, Inc. Liquid dispenser
US20180299476A1 (en) * 2017-04-12 2018-10-18 Bio-Rad Laboratories, Inc. Liquid dispenser and method of use

Also Published As

Publication number Publication date
JP2803809B2 (ja) 1998-09-24
JPH08266913A (ja) 1996-10-15

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