EP0700433A1 - Procede d'hydrolyse de proteines - Google Patents
Procede d'hydrolyse de proteinesInfo
- Publication number
- EP0700433A1 EP0700433A1 EP94913509A EP94913509A EP0700433A1 EP 0700433 A1 EP0700433 A1 EP 0700433A1 EP 94913509 A EP94913509 A EP 94913509A EP 94913509 A EP94913509 A EP 94913509A EP 0700433 A1 EP0700433 A1 EP 0700433A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- protein
- proteolytic
- preparation
- hydrolysis
- food product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 50
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 50
- 230000003301 hydrolyzing effect Effects 0.000 title claims abstract description 10
- 235000018102 proteins Nutrition 0.000 claims abstract description 48
- 238000002360 preparation method Methods 0.000 claims abstract description 41
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- 239000000796 flavoring agent Substances 0.000 claims abstract description 25
- 235000013351 cheese Nutrition 0.000 claims abstract description 21
- 235000019634 flavors Nutrition 0.000 claims abstract description 21
- 230000002797 proteolythic effect Effects 0.000 claims abstract description 21
- 108010009736 Protein Hydrolysates Proteins 0.000 claims abstract description 20
- 239000003531 protein hydrolysate Substances 0.000 claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 17
- 108091005804 Peptidases Proteins 0.000 claims abstract description 16
- 102000035195 Peptidases Human genes 0.000 claims abstract description 13
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- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 7
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 7
- 235000021120 animal protein Nutrition 0.000 claims abstract description 4
- 235000020256 human milk Nutrition 0.000 claims abstract description 4
- 239000002537 cosmetic Substances 0.000 claims abstract description 3
- 239000000284 extract Substances 0.000 claims abstract 2
- 239000000047 product Substances 0.000 claims description 18
- 102000004882 Lipase Human genes 0.000 claims description 14
- 108090001060 Lipase Proteins 0.000 claims description 14
- 239000004367 Lipase Substances 0.000 claims description 14
- 238000011534 incubation Methods 0.000 claims description 14
- 235000019421 lipase Nutrition 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000004365 Protease Substances 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- 102000011632 Caseins Human genes 0.000 claims description 6
- 108010076119 Caseins Proteins 0.000 claims description 6
- 108010011756 Milk Proteins Proteins 0.000 claims description 5
- 102000014171 Milk Proteins Human genes 0.000 claims description 5
- 229920000159 gelatin Polymers 0.000 claims description 5
- 235000019322 gelatine Nutrition 0.000 claims description 5
- 235000021239 milk protein Nutrition 0.000 claims description 5
- 230000007065 protein hydrolysis Effects 0.000 claims description 5
- 108091005658 Basic proteases Proteins 0.000 claims description 4
- 108010082495 Dietary Plant Proteins Proteins 0.000 claims description 4
- 108091005507 Neutral proteases Proteins 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- 230000002538 fungal effect Effects 0.000 claims description 4
- 108010017384 Blood Proteins Proteins 0.000 claims description 3
- 102000004506 Blood Proteins Human genes 0.000 claims description 3
- 240000002791 Brassica napus Species 0.000 claims description 3
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 3
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- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010028690 Fish Proteins Proteins 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 3
- 108010068370 Glutens Proteins 0.000 claims description 3
- 108010073032 Grain Proteins Proteins 0.000 claims description 3
- 108010070551 Meat Proteins Proteins 0.000 claims description 3
- 240000004658 Medicago sativa Species 0.000 claims description 3
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims description 3
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- 108010084695 Pea Proteins Proteins 0.000 claims description 3
- 244000000231 Sesamum indicum Species 0.000 claims description 3
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- 239000005018 casein Substances 0.000 claims description 3
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 3
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- 241000228212 Aspergillus Species 0.000 claims description 2
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- 241000235403 Rhizomucor miehei Species 0.000 claims 1
- 241000209140 Triticum Species 0.000 claims 1
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- 230000007062 hydrolysis Effects 0.000 abstract description 34
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 34
- 239000004614 Process Aid Substances 0.000 abstract 1
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- 235000013311 vegetables Nutrition 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 17
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- 229940088598 enzyme Drugs 0.000 description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
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- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 235000019626 lipase activity Nutrition 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 4
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
- 239000005695 Ammonium acetate Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 108010056079 Subtilisins Proteins 0.000 description 3
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- 235000019257 ammonium acetate Nutrition 0.000 description 3
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- 229940071440 soy protein isolate Drugs 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 235000013618 yogurt Nutrition 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 239000001828 Gelatine Substances 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
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- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/342—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of collagen; of gelatin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/343—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/343—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins
- A23J3/344—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins of casein
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/345—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of blood proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L15/00—Egg products; Preparation or treatment thereof
- A23L15/25—Addition or treatment with microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/21—Synthetic spices, flavouring agents or condiments containing amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/24—Synthetic spices, flavouring agents or condiments prepared by fermentation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21004—Trypsin (3.4.21.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21014—Microbial serine proteases (3.4.21.14)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
Definitions
- the present invention relates to a method of hydrolysing proteins, a protein hydrolysate obtained by the method, and food and non-food products containing the protein hydrolysate.
- the present invention provides a method for hydrolysis of proteins by incubation with a proteolytic preparation derived from Aspergrillus oryzae and supplied by Novo Nordisk A/S, Denmark under the tradename FlavourzymeTM.
- the invention provides protein hydrolysates obtained by the method of the invention.
- the invention provides food and non-food products comprising a protein hydrolysate of the invention.
- Fig. 1 shows the degree of hydrolysis (%DH) vs. time s of hydrolysis (hours) achieved by the method of the invention applied to sodium caseinate in the pH range of from pH 5 to pH 9 ( ⁇ : pH 5, A pH 6, O pH 7, D pH 8, • pH 9) ;
- Fig. 2 shows the degree of hydrolysis (%DH) of soy protein isolate after 22 hours of hydrolysis according to the ⁇ o method of invention ( ⁇ : FlavourzymeTM, A : CorolaseTM 7092, O: CorolaseTM 7093, ⁇ : AlcalaseTM, D: NeutraseTM) ; and
- Fig. 3 shows the degree of hydrolysis (%DH) of Na- caseinate after 22 hours of hydrolysis according to the method of invention ( ⁇ : FlavourzymeTM, A : CorolaseTM 7092, O: CorolaseTM is 7093, ⁇ : AlcalaseTM, D: NeutraseTM).
- proteolytic enzyme components each of which may have any of the following approximate molecular weights: 23 kD, 27 kD, 31 kD, 32 kD, 35 kD, 38 kD, 42 kD, 47 kD, 53 kD, and 100 kD.
- the present invention provides a method
- proteolytic enzyme preparation which is derived from Aspergillus oryzae and comprises at least five proteolytic components having an approximate molecular weight, respectively, selected from 23 kD, 27 kD, 31 kD, 32 kD, 35 kD, 38 kD, 42 kD, 47 kD, 53 kD, and
- the protein is incubated with a proteolytic preparation derived from Aspergillus oryzae and comprising at least five proteolytic components having the approximate molecular weights 23 kD, 31 kD, 35 kD, 38. kD, and
- the molecular weight of the protease components in the proteolytic preparation was determined by using SDS polyacrylamide gel electrophoresis (SDS-PAGE) in a manner known to persons skilled in the art. In this way, the molecular weight of each protease component was determined.
- the method of the invention is able to perform extensive protein hydrolysis of proteins, and the method leads to non-bitter hydrolysates and hydrolysates having pronounced soup-flavour/meat-flavour.
- the extent of the protein hydrolysis may be determined by the degree of hydrolysis achieved.
- the degree of hydrolysis (DH) is defined by the following formula:
- the DH may be calculated according to Adler-Nissen ;
- a DH which is higher than 35%, preferably higher than 60%, more preferably higher than 70%, most preferably higher than 80%.
- the degree of protein solubility may be described by way of a
- PSI Protein Solubility Index
- the protein or proteinaceous material which may advantageously be hydrolysed by the present method may be any vegetable protein such as soy protein, grain proteins, e.g. wheat gluten or zein, rape seed protein, alfalfa protein, pea protein, fabaceous bean protein, cotton seed protein or sesame seed protein, or any animal protein or proteinaceous material such as milk protein, whey protein, casein, meat protein, fish protein, blood protein, egg white or gelatin.
- vegetable protein such as soy protein, grain proteins, e.g. wheat gluten or zein, rape seed protein, alfalfa protein, pea protein, fabaceous bean protein, cotton seed protein or sesame seed protein
- animal protein or proteinaceous material such as milk protein, whey protein, casein, meat protein, fish protein, blood protein, egg white or gelatin.
- the proteolytic enzyme may suitably be added to the protein or proteinaceous material in an amount of 0.05-15 AU/100 g of protein, in particular 0.1-8 AU/100 g of protein.
- the incubation may be performed at a pH from between about 4 and about 10, preferably between about 5 and about 9.
- the method of the invention performs excellent even at extreme pH conditions, i.e. at pH values in all of the range of 5 to 9.
- the incubation may be performed at any convenient temperature at which the enzyme preparation does not become inactivated, i.e. in the range of from about 20°C to about 70°C.
- the proteo ⁇ lytic enzyme preparation may suitably be inactivated by increasing the temperature of the incubation mixture to above about 70°C, or by decreasing the pH of the incubation mixture to below about 4.0.
- incubation of a protein or proteinaceous substrate may be performed with a combination of FlavourzymeTM and one or more other protease preparations.
- Preferred protease preparations comprises neutral or alkaline proteases.
- suitable neutral proteases are neutral proteases derived from Bacillus, preferably from Bacillus ⁇ ubtilis, such as the enzyme preparation supplied by Novo Nordisk, Denmark, under the tradename NeutraseTM.
- alkaline proteases examples include alkaline proteases derived from Bacillus , preferably from Bacillus licheniformis, such as the enzyme preparation supplied by Novo Nordisk, Denmark, under the tradename AlcalaseTM and containing Subtilisin A (Subtilisin Carlsberg) as the active component.
- AlcalaseTM alkaline proteases derived from Bacillus , preferably from Bacillus licheniformis, such as the enzyme preparation supplied by Novo Nordisk, Denmark, under the tradename AlcalaseTM and containing Subtilisin A (Subtilisin Carlsberg) as the active component.
- the incubation carried out in the method of the invention may also be performed with a combination of FlavourzymeTM and one or more other lipase preparations.
- Preferred lipase preparations comprises fungal lipases.
- Suitable fungal lipases are lipases derived from Mucor, preferably from .Rhizojnucor miehei , such as the enzyme preparation supplied by Novo Nordisk, Denmark, under the tradename PalataseTMM; and lipases derived from Aspergillus , preferably from Aspergillus niger such as the enzyme preparation supplied by Novo Nordisk, Denmark, under the tradename PalataseTMA.
- the present invention provides a protein hydrolysate obtained by the method of the invention.
- the proteolytic activity may be determined with haemoglobin as substrate.
- TCA trichloroacetic acid
- a folder AF 4/5 describing the analytical method in more detail is available from Novo Nordisk A/S, DK-2880 Bagsvaerd, Denmark, upon request which folder is hereby included by reference.
- a substrate for lipase was prepared by emulsifying glycerine tributyrat (MERCK) using gum-arabic as emulsifier. Lipase activity was assayed at pH 7 using pH stat method. One unit of lipase activity (LU/mg) is defined as the amount needed to liberate one micro ole fatty acid per minute.
- Step 1 Centrifuge the fermentation supernatant, discard the precipitate. Adjust the pH of the supernatant to 7 and add gradually an equal volume of cold 96 % ethanol. Allow the mixture to stand for 30 minutes in an ice bath. Centrifuge and discard the precipitate.
- Step 2 - Ion exchange chromatography. Filter the supernatant and apply on DEAE-fast flow (Pharmacia TM) column equilibrated with 50 mM tris-acetate buffer pH 7. Wash the column with the same buffer till absorption at 280 nm is lower than 0.05 OD. Elute the bound enzymatic activity with linear salt gradient in the same buffer (0 to 0.5 M NaCl ) using five column volumes, Pool the fractions containing enzymatic activity .
- Step 3 Hydrophobic chromatography. Adjust the molarity of the pool containing enzymatic activity to 0.8 M by adding solid Ammonium acetate. Apply the enzyme on TSK gel Butyl- Toyopearl 650 C column (available from Tosoh Corporation Japan) which was pre-equilibrated with 0.8 M ammonium acetate. Wash the unbound material with 0.8 M ammonium acetate and elute the bound material with distilled water.
- Step 4 Pool containing lipase activity is diluted with water to adjust conductance to 2 mS and pH to 7. Apply the pool on High performance Q Sepharose (Pharmacia) column pre- equilibrated with 50 mM tris -acetate buffer pH 7. Elute the bound enzyme with linear salt gradient.
- the present invention relates to food products comprising a protein hydrolysate of the inven ⁇ tion.
- the amount of protein hydrolysate incorporated in the food product will typically be in the range of 1-30% by weight.
- An important food product of the present invention is an ingredient of a mother milk substitute for infants. Due to the high degree of hydrolysis obtained by the method of the invention, the protein hydrolysates of the invention may advan- tageously be incorporated in mother milk substitutes, the hydrolysate having a significantly lower allergenicity than unhydrolysed milk proteins.
- the milk substitute may be for ⁇ mulated in substantially the same way as that indicated in the prior literature for products of this type (cf. for instance EP Patent Application 322,589) with the exception that the protein hydrolysate included in the known products is replaced by the protein hydrolysate of the present invention.
- the food product of the invention may also include the protein hydrolysate of the invention as a protein supple- ment or to provide other properties of the food product.
- the protein hydrolysate incorporated in the food product may for instance be based on meat or scrap meat (e.g. the so-called mechanically recovered meat, i.e. meat remaining on bones after the regular pieces of meat have been carved from animal carcases in the slaughterhouse.
- meat or scrap meat e.g. the so-called mechanically recovered meat, i.e. meat remaining on bones after the regular pieces of meat have been carved from animal carcases in the slaughterhouse.
- meat or scrap meat e.g. the so-called mechanically recovered meat, i.e. meat remaining on bones after the regular pieces of meat have been carved from animal carcases in the slaughterhouse.
- mechanically recovered meat i.e. meat remaining on bones after the regular pieces of meat have been carved from animal carcases in the slaughterhouse.
- Other proteinaceous by-products from the meat industry may
- the resulting protein hydrolysate may then suitably be added to emulsified meat products, e.g. sausages or pates, or as flavour ingredients in soups or other food products.
- a food product obtained from cow milk by the method of invention is preferably a cheese flavour product.
- the extensive hydrolysis of the milk protein surprisingly leads to flavour compounds with very distinct cheese flavour.
- Such cheese flavour products of the invention preferably find application in snack products, imitation cheese products, or as enhancers of cheese flavour in general.
- the cheese flavour may be adjusted by use of lipases according to well established practice.
- a traditional way of producing Hydrolysed Vegetable Protein (HVP) for use as flavour products is based on cooking in hydrochloric acid at a high temperature and for a long time. This is known to cause unwanted formation of chloric containing compounds. By the process of the invention it is now possible to obtain enzymatically produced HVP products, which are considered more healthy products.
- the high DH obtainable by the process of the invention leads to desirable flavour characteristics and flavour enhancing properties.
- the process of the invention may also be used for protein enrichment of dietetic products due to the non-bitter flavour obtained by the process.
- Also advantageous is the very high protein solubility in a very broad pH range that is associated with the process of the invention. It has surprisingly been found that an excellent fermentation medium may be produced by subjecting milk to the method of the present invention. Such milk hydrolysates may be used for acceleration of the fermentation in yoghurt production or in the production of lactic acid starter bacteria cultures, by addition in relatively small amounts. The shortening of process time increases production capacity and reduces the risk of infection, e.g. bacteriophage infection.
- the method of the invention may be used in combination with a fermentation process, preferably a fermentation process for the production of a food product.
- the proteolytic enzyme preparation e.g. FlavourzymeTM
- the protein hydrolysate of the invention or both may be added in a fermentation process, preferably a food fermentation process, for enhancing the productivity of the fermentation process.
- fermentation processes are processes involving or fermenting fish (fish sauce) , cocoa beans or soy (such as soy sauce, tempeh, iso) .
- the addition of the proteolytic enzyme preparation or the addition of protein hydrolysate may be useful for decreasing the total process time, i.e. increasing the rate of fermentation.
- Non-food products e.g. FlavourzymeTM
- the process of the invention may also find application in the non-food area.
- the flavour characteristics obtained by the method of invention may advantageously be used for production 5 of pet food.
- Preparation B 1% FlavourzymeTM, 2% NeutraseTM 0.5L, and 0.15% AlcalaseTM 2,4L.
- FlavourzymeTM is a proteolytic preparation derived from
- NeutraseTM 0.5L is a proteolytic preparation derived from Bacillus subtilis
- AlcalaseTM 2.4L is a proteolytic preparation derived from Bacillus licheniformis .
- hydrolysates were served before a tasting panel in a 4% solution.
- the meat flavour was most pronounced in hydrolysates obtained with Preparation A.
- flavour-samples 1, 2, and 3 were now diluted in cream cheese, and a fourth sample was prepared as a control.
- sample No. 4.1 6.0 g of fresh milk + 20 g of cheese.
- Sample No. 1.1 (FlavourzymeTM) appeared to have a very strong and intense flavour and a taste of well ripened cheese.
- Sample No. 2.1 (FlavourzymeTM + PalataseTM) appeared to have a 0 well balanced cheese flavour.
- sample No. 3.1 turned out to give the weakest taste impression.
- sample No. 4.1 was evaluated being sour/sourish.
- PalataseTM creates a new and much more simple way of producing cheese flavour.
- the hydrolysis was carried out for 20 hours at 50°C. Then the enzymes were denatured by heat treatment at 80°C for 5 min.
- Skim milk was heat treated at 90°C for 2 min and three samples of 200 ml were prepared.
- the temperature was adjusted to 41°C, and yoghurt culture YC DVS from Chr. Hansens Lab. was added to all three samples in a level of 10 6 bacteria per g.
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Abstract
L'invention concerne un procédé d'hydrolyse d'une protéine végétale ou animale par incubation avec une préparation à base d'enzymes protéolytiques dérivée de l'Aspergillus oryzae telle que la FlavourzymeTM comprenant au moins cinq composés protéolytiques présentant une masse molaire approximative de 23 kD, 27 kD, 31 kD, 32 kD, 38 kD, 42 kD, 47 kD, 53 kD ou de 100 kD, qui permet de produire un hydrolysat protéique utile dans ou comme un produit alimentaire tel que le lait maternel de substitution, le fromage, la protéine végétale hydrolysée, un aromatisant et un adjuvant de procédé pour la fermentation de produits alimentaires ou un produit non alimentaire tel qu'un aliment pour animaux familiers, des cosmétiques. Le procédé selon l'invention permet d'obtenir un degré d'hydrolyse et de goût élevés et solubilité protéique élevée.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK93467A DK46793D0 (da) | 1993-04-26 | 1993-04-26 | Enzym |
DK467/93 | 1993-04-26 | ||
PCT/DK1994/000165 WO1994025580A1 (fr) | 1993-04-26 | 1994-04-25 | Procede d'hydrolyse de proteines |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0700433A1 true EP0700433A1 (fr) | 1996-03-13 |
Family
ID=8093877
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP94913509A Withdrawn EP0700433A1 (fr) | 1993-04-26 | 1994-04-25 | Procede d'hydrolyse de proteines |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0700433A1 (fr) |
JP (1) | JPH08509366A (fr) |
KR (1) | KR960701993A (fr) |
CN (1) | CN1090675C (fr) |
AU (1) | AU681653B2 (fr) |
DK (1) | DK46793D0 (fr) |
NZ (1) | NZ265247A (fr) |
WO (1) | WO1994025580A1 (fr) |
Families Citing this family (69)
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ATE257329T1 (de) * | 1994-10-26 | 2004-01-15 | Novozymes As | Verfahren für die herstellung eines milch-protein-hydrolysates |
DE19632455C1 (de) * | 1996-08-12 | 1997-08-21 | Cpc Maizena Gmbh | Verfahren zur Herstellung eines Proteinhydrolysats aus proteinhaltigen tierischen Produkten |
EP0936875B1 (fr) * | 1996-10-30 | 2003-06-25 | Novozymes A/S | Procede de preparation d'un agent aromatisant alimentaire |
AU732747B2 (en) * | 1996-10-30 | 2001-04-26 | Novozymes A/S | Method of producing food flavoring agent |
ES2116937B1 (es) * | 1996-11-29 | 1999-03-01 | Consejo Superior Investigacion | Procedimiento de obtencion de peptonas vegetales de alto grado de hidrolisis y sus aplicaciones. |
DK0946106T3 (da) * | 1996-12-23 | 2002-09-16 | Dsm Nv | Fremgangsmåde til fremstilling af et proteinhydrolysat |
CA2275423A1 (fr) * | 1996-12-23 | 1998-07-02 | Dsm N.V. | Renforcateur de gout |
US6007851A (en) * | 1996-12-23 | 1999-12-28 | Gist-Brocades, B.V. | Process for producing a flavor enhancer |
WO1998051163A2 (fr) * | 1997-05-16 | 1998-11-19 | Novo Nordisk Biotech, Inc. | Procedes de production d'hydrolysats proteiques |
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DE3306009C2 (de) * | 1983-02-22 | 1994-02-17 | Roehm Gmbh | Verfahren zur Herstellung von Proteinhydrolysaten |
CN86108557A (zh) * | 1986-12-20 | 1988-07-13 | 同济大学 | 以含蛋白质的废料为原料酶法提取蛋白质水解物的方法及其产品 |
CH679542A5 (fr) * | 1989-11-27 | 1992-03-13 | Nestle Sa |
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- 1994-04-25 AU AU65640/94A patent/AU681653B2/en not_active Ceased
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- 1994-04-25 EP EP94913509A patent/EP0700433A1/fr not_active Withdrawn
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DK46793D0 (da) | 1993-04-26 |
CN1090675C (zh) | 2002-09-11 |
NZ265247A (en) | 1996-07-26 |
JPH08509366A (ja) | 1996-10-08 |
AU6564094A (en) | 1994-11-21 |
KR960701993A (ko) | 1996-03-28 |
AU681653B2 (en) | 1997-09-04 |
CN1121732A (zh) | 1996-05-01 |
WO1994025580A1 (fr) | 1994-11-10 |
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