EA201891629A1 - Способы и композиции для повышения эффективности нацеленной модификации генов с применением опосредуемой олигонуклеотидами репарации генов - Google Patents

Способы и композиции для повышения эффективности нацеленной модификации генов с применением опосредуемой олигонуклеотидами репарации генов

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Publication number
EA201891629A1
EA201891629A1 EA201891629A EA201891629A EA201891629A1 EA 201891629 A1 EA201891629 A1 EA 201891629A1 EA 201891629 A EA201891629 A EA 201891629A EA 201891629 A EA201891629 A EA 201891629A EA 201891629 A1 EA201891629 A1 EA 201891629A1
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Eurasian Patent Office
Prior art keywords
genes
compositions
methods
improving
efficiency
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EA201891629A
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English (en)
Inventor
Питер Р. Битэм
Грегори Ф.В. Гокал
Кристиан Шопке
Ноэль Сауэр
Джеймс Пирс
Роза Е. Сегами
Джерри Мозорук
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Сибас Юс Ллс
Сибас Юроп Б.В.
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Application filed by Сибас Юс Ллс, Сибас Юроп Б.В. filed Critical Сибас Юс Ллс
Publication of EA201891629A1 publication Critical patent/EA201891629A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

Согласно настоящему изобретению предложены способы и композиции для внесения нацеленных изменений в последовательность ДНК. В различных аспектах и вариантах реализации предложены способы и композиции для модификации последовательности ДНК в клетке (такой как клетка растений, бактерий, дрожжей, грибов, водорослей или млекопитающих). В некоторых аспектах и вариантах реализации модификация ДНК включает комбинирование олигонуклеотидов для репарации генов с подходами, которые повышают доступность компонентов механизмов репарации генов клетки-мишени, таких как агент для разрезания ДНК.
EA201891629A 2016-02-09 2016-09-16 Способы и композиции для повышения эффективности нацеленной модификации генов с применением опосредуемой олигонуклеотидами репарации генов EA201891629A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662293278P 2016-02-09 2016-02-09
PCT/US2016/052346 WO2017138986A1 (en) 2016-02-09 2016-09-16 Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair

Publications (1)

Publication Number Publication Date
EA201891629A1 true EA201891629A1 (ru) 2019-03-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201891629A EA201891629A1 (ru) 2016-02-09 2016-09-16 Способы и композиции для повышения эффективности нацеленной модификации генов с применением опосредуемой олигонуклеотидами репарации генов

Country Status (9)

Country Link
US (2) US11542515B2 (ru)
JP (2) JP2019506170A (ru)
KR (1) KR20180116769A (ru)
CN (1) CN109072248A (ru)
BR (1) BR112018016278A2 (ru)
CA (1) CA3014036A1 (ru)
CL (1) CL2018002140A1 (ru)
EA (1) EA201891629A1 (ru)
WO (1) WO2017138986A1 (ru)

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CN111801007A (zh) 2017-12-28 2020-10-20 嘉士伯有限公司 具有增加的水解酶活性的大麦
US11690341B2 (en) 2017-12-28 2023-07-04 Carlsberg A/S Cereal plants with improved cell wall properties
WO2019134962A1 (en) 2018-01-05 2019-07-11 Carlsberg A/S Cereal comprising starch with low gelatinisation temperature
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CN112375781B (zh) * 2020-11-08 2023-05-02 浙江大学 pC1300-MAS-Cas9基因编辑***在84K杨树基因编辑中的应用
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Also Published As

Publication number Publication date
BR112018016278A2 (pt) 2019-01-02
JP2019506170A (ja) 2019-03-07
JP2022153467A (ja) 2022-10-12
KR20180116769A (ko) 2018-10-25
CN109072248A (zh) 2018-12-21
US20230407322A1 (en) 2023-12-21
CA3014036A1 (en) 2017-08-17
CL2018002140A1 (es) 2019-01-04
US11542515B2 (en) 2023-01-03
WO2017138986A1 (en) 2017-08-17
US20190249182A1 (en) 2019-08-15

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