EA201791118A1 - Кодон-оптимизированный полинуклеотид для экспрессии crmна высоком уровне - Google Patents
Кодон-оптимизированный полинуклеотид для экспрессии crmна высоком уровнеInfo
- Publication number
- EA201791118A1 EA201791118A1 EA201791118A EA201791118A EA201791118A1 EA 201791118 A1 EA201791118 A1 EA 201791118A1 EA 201791118 A EA201791118 A EA 201791118A EA 201791118 A EA201791118 A EA 201791118A EA 201791118 A1 EA201791118 A1 EA 201791118A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- crm
- expression
- high level
- codon
- polynucleotide
- Prior art date
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/385—Haptens or antigens, bound to carriers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1077—Pentosyltransferases (2.4.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Food Science & Technology (AREA)
- Epidemiology (AREA)
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Abstract
Настоящее изобретение относится к экспрессии на высоком уровне бактериального токсоида или белка-токсина, представляющего фармакологический интерес, посредством оптимизированной новой полинуклеотидной последовательности, и к хозяину, трансформированному указанным полинуклеотидом. В частности, изобретение относится к способу высокой продукции полипептида CRM, где полинуклеотид по изобретению используют для трансформации подходящего хозяина, что приводит к сверхэкспрессии соответствующих белков, и к способу выделения экспрессируемого полипептида. Более конкретно, настоящее изобретение относится к экспрессии CRMна высоком уровне Escherichia coli и к способу его выделения и очистки.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN4045CH2014 | 2014-11-20 | ||
PCT/IN2015/000427 WO2016079755A1 (en) | 2014-11-20 | 2015-11-17 | Codon optimized polynucleotide for high level expression of crm197 |
Publications (2)
Publication Number | Publication Date |
---|---|
EA201791118A1 true EA201791118A1 (ru) | 2017-10-31 |
EA035117B1 EA035117B1 (ru) | 2020-04-29 |
Family
ID=55346156
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201791118A EA035117B1 (ru) | 2014-11-20 | 2015-11-17 | Кодон-оптимизированный полинуклеотид для экспрессии crmна высоком уровне |
Country Status (11)
Country | Link |
---|---|
US (1) | US10280409B2 (ru) |
EP (1) | EP3221339A1 (ru) |
JP (2) | JP2017538444A (ru) |
CN (1) | CN107109416A (ru) |
AU (1) | AU2015348922B2 (ru) |
CA (1) | CA2968491C (ru) |
EA (1) | EA035117B1 (ru) |
MX (1) | MX2017006506A (ru) |
SA (1) | SA517381488B1 (ru) |
WO (1) | WO2016079755A1 (ru) |
ZA (1) | ZA201703150B (ru) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11147863B2 (en) | 2015-06-23 | 2021-10-19 | Biological E Limited | Multivalent pneumococcal conjugate vaccine |
NZ752348A (en) | 2016-09-30 | 2022-11-25 | Biological E Ltd | Multivalent pneumococcal vaccine compositions comprising polysaccharide-protein conjugates |
WO2019043593A1 (en) * | 2017-09-01 | 2019-03-07 | Lorven Biologics Private Limited | NUCLEIC ACID ENCODING CRM197 AND ENHANCED EXPRESSION PROCESS THEREOF |
KR20200111685A (ko) * | 2018-01-19 | 2020-09-29 | 오비아이 파머 인코퍼레이티드 | Crm197 단백질 발현 |
WO2020033398A1 (en) * | 2018-08-06 | 2020-02-13 | The Trustees Of Columbia University In The City Of New York | Compositions and methods for treating atii cell-dependent lung diseases |
KR102142255B1 (ko) * | 2019-12-05 | 2020-08-07 | 주식회사 제노포커스 | Crm197 단백질 발현 방법 |
GB202108650D0 (en) * | 2021-06-17 | 2021-08-04 | Inst De Medicina Molecular Joaeo Lobo Antunes | Production of cross-reactive material 197 fusion proteins |
KR20230102682A (ko) * | 2021-12-30 | 2023-07-07 | (주)셀트리온 | 목적 단백질의 발현 방법 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
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US4755465A (en) | 1983-04-25 | 1988-07-05 | Genentech, Inc. | Secretion of correctly processed human growth hormone in E. coli and Pseudomonas |
US5281532A (en) | 1983-07-27 | 1994-01-25 | Mycogen Corporation | Pseudomas hosts transformed with bacillus endotoxin genes |
US4695462A (en) | 1985-06-28 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of biological pesticides |
US4695455A (en) | 1985-01-22 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of pesticides produced by expression of heterologous genes |
US5128130A (en) | 1988-01-22 | 1992-07-07 | Mycogen Corporation | Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens |
US5055294A (en) | 1988-03-03 | 1991-10-08 | Mycogen Corporation | Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens |
US5169760A (en) | 1989-07-27 | 1992-12-08 | Mycogen Corporation | Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters |
ES2231770T3 (es) | 1993-03-05 | 2005-05-16 | Wyeth Holdings Corporation | Nuevos plasmidos para la produccion de proteina crm y toxina difterica. |
US20060270600A1 (en) | 2005-05-26 | 2006-11-30 | Eisuke Mekada | Anti-cancer agents |
NZ569168A (en) * | 2005-12-22 | 2012-02-24 | Glaxosmithkline Biolog Sa | Streptococcus pneumoniae polysaccharide conjugate vaccine comprising 19F-DT conjugate |
IT1398927B1 (it) * | 2009-06-25 | 2013-03-28 | Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi | Espressione batterica di un gene artificiale per la produzione di crm197 e derivati. |
GB0917647D0 (en) * | 2009-10-08 | 2009-11-25 | Glaxosmithkline Biolog Sa | Expression system |
JP5839411B2 (ja) | 2010-03-30 | 2016-01-06 | フェニックス インク. | 組み換え型毒素タンパク質の高レベルの発現 |
GB201106225D0 (en) | 2011-04-13 | 2011-05-25 | Glaxosmithkline Biolog Sa | Fermentation process |
GB201209896D0 (en) | 2012-06-01 | 2012-07-18 | Fujifilm Diosynth Biotechnologies Uk Ltd | Process |
CN102766647A (zh) * | 2012-07-25 | 2012-11-07 | 天津康希诺生物技术有限公司 | 在白喉杆菌中稳定复制的表达载体及含该载体的白喉杆菌 |
EP2938363B1 (en) * | 2012-12-27 | 2019-08-21 | GlaxoSmithKline Biologicals S.A. | Methods and compositions relating to crm197 |
CN103266125A (zh) * | 2013-06-04 | 2013-08-28 | 北京民海生物科技有限公司 | 白喉毒素突变体crm197的制备方法 |
KR102315246B1 (ko) | 2014-03-03 | 2021-10-21 | 스캐랩 게노믹스, 엘엘씨 | 대장균에서 재조합 crm197의 향상된 생산 |
-
2015
- 2015-11-17 MX MX2017006506A patent/MX2017006506A/es unknown
- 2015-11-17 US US15/523,616 patent/US10280409B2/en active Active
- 2015-11-17 CA CA2968491A patent/CA2968491C/en active Active
- 2015-11-17 CN CN201580062524.6A patent/CN107109416A/zh active Pending
- 2015-11-17 JP JP2017546275A patent/JP2017538444A/ja active Pending
- 2015-11-17 AU AU2015348922A patent/AU2015348922B2/en active Active
- 2015-11-17 EP EP15832894.8A patent/EP3221339A1/en active Pending
- 2015-11-17 WO PCT/IN2015/000427 patent/WO2016079755A1/en active Application Filing
- 2015-11-17 EA EA201791118A patent/EA035117B1/ru unknown
-
2017
- 2017-05-08 ZA ZA2017/03150A patent/ZA201703150B/en unknown
- 2017-05-09 SA SA517381488A patent/SA517381488B1/ar unknown
-
2020
- 2020-07-03 JP JP2020115624A patent/JP7042305B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
EA035117B1 (ru) | 2020-04-29 |
EP3221339A1 (en) | 2017-09-27 |
MX2017006506A (es) | 2018-03-12 |
AU2015348922B2 (en) | 2020-01-23 |
JP2020171307A (ja) | 2020-10-22 |
WO2016079755A1 (en) | 2016-05-26 |
AU2015348922A8 (en) | 2019-03-14 |
JP7042305B2 (ja) | 2022-03-25 |
US10280409B2 (en) | 2019-05-07 |
BR112017010718A2 (pt) | 2017-12-26 |
SA517381488B1 (ar) | 2021-10-17 |
AU2015348922A1 (en) | 2017-06-01 |
US20170306302A1 (en) | 2017-10-26 |
CA2968491C (en) | 2023-09-26 |
CN107109416A (zh) | 2017-08-29 |
ZA201703150B (en) | 2019-05-29 |
JP2017538444A (ja) | 2017-12-28 |
CA2968491A1 (en) | 2016-05-26 |
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