EA025732B9 - Резистентные к гербицидам ahas-мутанты и способы применения - Google Patents

Резистентные к гербицидам ahas-мутанты и способы применения

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Publication number
EA025732B9
EA025732B9 EA201170490A EA201170490A EA025732B9 EA 025732 B9 EA025732 B9 EA 025732B9 EA 201170490 A EA201170490 A EA 201170490A EA 201170490 A EA201170490 A EA 201170490A EA 025732 B9 EA025732 B9 EA 025732B9
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Eurasian Patent Office
Prior art keywords
methods
mutants
nucleic acids
plants
ahas
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EA201170490A
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English (en)
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EA201170490A1 (ru
EA025732B1 (ru
Inventor
Питер Битхэм
Дейл Карлсон
Грег Гоукал
Джон Мак Элвер
Джеймс Пирс
Кристиан Шопке
Биджай Сингх
Кит Уолкер
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Басф Агрокемикал Продактс Б.В.
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Publication of EA201170490A1 publication Critical patent/EA201170490A1/ru
Publication of EA025732B1 publication Critical patent/EA025732B1/ru
Publication of EA025732B9 publication Critical patent/EA025732B9/ru

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278Sulfonylurea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Catching Or Destruction (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение относится к трансгенным или нетрансгенным растениям с повышенными уровнями устойчивости к AHAS-ингибирующим гербицидам. Изобретение также относится к нуклеиновым кислотам, кодирующим мутанты большой субъединицы синтазы ацетогидроксикислот (AHAS), к экспрессирующим векторам, к растениям, содержащим нуклеиновые кислоты, кодирующие субъединицы AHASL, содержащие одну, две или большее количество мутаций, к растениям, содержащим такие мутантные нуклеиновые кислоты субъединицы AHASL, к способам их получения и применения и к способам борьбы с сорняками.
EA201170490A 2008-09-26 2009-09-24 Резистентные к гербицидам ahas-мутанты и способы применения EA025732B9 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10054108P 2008-09-26 2008-09-26
PCT/US2009/058169 WO2010036771A2 (en) 2008-09-26 2009-09-24 Herbicide-resistant ahas-mutants and methods of use

Publications (3)

Publication Number Publication Date
EA201170490A1 EA201170490A1 (ru) 2011-12-30
EA025732B1 EA025732B1 (ru) 2017-01-30
EA025732B9 true EA025732B9 (ru) 2017-09-29

Family

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Family Applications (2)

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EA201170490A EA025732B9 (ru) 2008-09-26 2009-09-24 Резистентные к гербицидам ahas-мутанты и способы применения
EA201691309A EA201691309A1 (ru) 2008-09-26 2009-09-24 Резистентные к гербицидам ahas-мутанты и способы применения

Family Applications After (1)

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Country Status (20)

Country Link
US (1) US20120060243A1 (ru)
EP (3) EP2733212B1 (ru)
JP (2) JP6133011B2 (ru)
KR (1) KR20110080154A (ru)
CN (2) CN102216453B (ru)
AP (1) AP2011005671A0 (ru)
AR (1) AR073402A1 (ru)
AU (2) AU2009296625B2 (ru)
BR (1) BRPI0919380A2 (ru)
CA (1) CA2737939C (ru)
CL (1) CL2011000665A1 (ru)
EA (2) EA025732B9 (ru)
GE (1) GEP20146179B (ru)
MX (1) MX2011003266A (ru)
NZ (2) NZ592464A (ru)
PL (2) PL2733212T3 (ru)
UA (1) UA115019C2 (ru)
UY (1) UY32144A (ru)
WO (3) WO2010036771A2 (ru)
ZA (1) ZA201102076B (ru)

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CN104126496B (zh) * 2014-07-03 2016-08-24 华中农业大学 利用抗除草剂基因的油菜化学杀雄制种方法
CN104531598B (zh) * 2015-01-08 2018-01-16 齐鲁工业大学 一种重组链霉菌、其构建方法及提高抗生素产量的方法
WO2016130247A1 (en) * 2015-01-10 2016-08-18 Cibus Us Llc Mutated acetohydroxyacid synthase genes in euphorbiaceae and plant material comprising such genes
JP2017118878A (ja) * 2017-01-16 2017-07-06 アドヴァンタ・ホールディングス・ベスローテン・フェンノートシャップ 突然変異アセトヒドロキシ酸合成酵素タンパク質のラージサブユニットをコードする突然変異ポリヌクレオチドを有し、除草剤耐性が増大したソルガム植物
CN110545659A (zh) * 2017-01-31 2019-12-06 赖斯泰克有限公司 多个突变对提高水稻中的除草剂抗性/耐受性的作用
JP2019141045A (ja) * 2019-03-04 2019-08-29 アドヴァンタ・ホールディングス・ベスローテン・フェンノートシャップ 突然変異アセトヒドロキシ酸合成酵素タンパク質のラージサブユニットをコードする突然変異ポリヌクレオチドを有し、除草剤耐性が増大したソルガム植物
KR102147381B1 (ko) * 2019-11-22 2020-08-24 씨제이제일제당 주식회사 아세토하이드록시산 신타제 신규 변이체 및 이를 포함하는 미생물
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