DK3546924T3 - System til tælling af mikrobielle partikler og fremgangsmåde til tælling af mikrobielle partikler - Google Patents

System til tælling af mikrobielle partikler og fremgangsmåde til tælling af mikrobielle partikler Download PDF

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Publication number
DK3546924T3
DK3546924T3 DK17874689.7T DK17874689T DK3546924T3 DK 3546924 T3 DK3546924 T3 DK 3546924T3 DK 17874689 T DK17874689 T DK 17874689T DK 3546924 T3 DK3546924 T3 DK 3546924T3
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DK
Denmark
Prior art keywords
particulate counting
microbial particulate
microbial
counting method
counting system
Prior art date
Application number
DK17874689.7T
Other languages
English (en)
Inventor
Kazuma Sekimoto
Original Assignee
Rion Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2017204059A external-priority patent/JP6954800B2/ja
Application filed by Rion Co filed Critical Rion Co
Application granted granted Critical
Publication of DK3546924T3 publication Critical patent/DK3546924T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/34Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/02Means for providing, directing, scattering or concentrating light located outside the reactor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06Quantitative determination
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N15/0205Investigating particle size or size distribution by optical means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06Investigating concentration of particle suspensions
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65Raman scattering
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N15/0205Investigating particle size or size distribution by optical means
    • G01N2015/0238Single particle scatter
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486Counting the particles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1488Methods for deciding
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493Particle size

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DK17874689.7T 2016-11-22 2017-11-07 System til tælling af mikrobielle partikler og fremgangsmåde til tælling af mikrobielle partikler DK3546924T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2016227169 2016-11-22
JP2017204059A JP6954800B2 (ja) 2016-11-22 2017-10-20 生物粒子計数システムおよび生物粒子計数方法
PCT/JP2017/040087 WO2018096920A1 (ja) 2016-11-22 2017-11-07 生物粒子計数システムおよび生物粒子計数方法

Publications (1)

Publication Number Publication Date
DK3546924T3 true DK3546924T3 (da) 2022-05-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK17874689.7T DK3546924T3 (da) 2016-11-22 2017-11-07 System til tælling af mikrobielle partikler og fremgangsmåde til tælling af mikrobielle partikler

Country Status (4)

Country Link
EP (1) EP3546924B1 (da)
CN (1) CN110023741B (da)
DK (1) DK3546924T3 (da)
WO (1) WO2018096920A1 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022107070A (ja) * 2019-05-13 2022-07-21 国立研究開発法人海洋研究開発機構 粒子分取装置、粒子分取方法及びコンピュータプログラム
WO2022055761A1 (en) * 2020-09-11 2022-03-17 Amgen Inc. Inline uv flow device for sterilizing flow cytometry sheath fluid

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* Cited by examiner, † Cited by third party
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US20020028434A1 (en) * 2000-09-06 2002-03-07 Guava Technologies, Inc. Particle or cell analyzer and method
JP3995684B2 (ja) * 2004-12-21 2007-10-24 リオン株式会社 粒子計数器
US7741108B2 (en) * 2005-01-14 2010-06-22 Optech Ventures, Llc Bacteria sensor and method
JP5112312B2 (ja) * 2005-07-15 2013-01-09 バイオヴィジラント システムズ インコーポレイテッド 病原体及び微粒子検出システム並びに検出法
WO2008136769A1 (en) * 2007-05-03 2008-11-13 Nanyang Technological University Online contaminant detection and removal system
CN101398367B (zh) * 2007-09-26 2011-04-06 中国人民解放军军事医学科学院微生物流行病研究所 气溶胶粒子激光分析仪
ES2586623T3 (es) * 2008-08-15 2016-10-17 Aperture Bio, LLC Sistemas y métodos basados en citometría de flujo para la detección de microbios
EP2194368B1 (de) * 2008-12-03 2019-07-17 Grundfos Management A/S Sensorsystem zum Erfassen und Spezifizieren von einzelnen Partikeln in einem Fluid
JP2012217382A (ja) * 2011-04-08 2012-11-12 Fuji Electric Co Ltd 微生物検出装置
US20130015362A1 (en) * 2011-07-12 2013-01-17 Sharp Kabushiki Kaisha Fluid purification and sensor system
JP6126400B2 (ja) 2013-02-08 2017-05-10 リオン株式会社 生物粒子計数システム及び生物粒子計数方法
JP6121319B2 (ja) * 2013-03-29 2017-04-26 シスメックス株式会社 粒子測定装置、照射光学系および照射位置調整方法
JP6314454B2 (ja) * 2013-12-04 2018-04-25 横河電機株式会社 細胞検査装置および細胞検査方法
CN103940709A (zh) * 2014-05-06 2014-07-23 南京中科神光科技有限公司 一种实时微生物粒子计数器
JP6425918B2 (ja) * 2014-05-27 2018-11-21 アズビル株式会社 精製水製造装置の監視システム及び精製水製造装置の監視方法
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JP6325423B2 (ja) * 2014-10-10 2018-05-16 アズビル株式会社 液中蛍光検出装置及び液中の蛍光の検出方法
JP2016111940A (ja) * 2014-12-11 2016-06-23 アズビル株式会社 代謝活性を有する微生物の検出装置
JP6240280B1 (ja) * 2016-08-26 2017-11-29 リオン株式会社 生物粒子計数システムおよび生物粒子計数方法

Also Published As

Publication number Publication date
EP3546924A1 (en) 2019-10-02
CN110023741A (zh) 2019-07-16
EP3546924A4 (en) 2019-12-18
WO2018096920A1 (ja) 2018-05-31
CN110023741B (zh) 2022-01-28
EP3546924B1 (en) 2022-03-30

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