DK3183340T3 - Termolabile exonukleaser - Google Patents

Termolabile exonukleaser Download PDF

Info

Publication number
DK3183340T3
DK3183340T3 DK15770444.6T DK15770444T DK3183340T3 DK 3183340 T3 DK3183340 T3 DK 3183340T3 DK 15770444 T DK15770444 T DK 15770444T DK 3183340 T3 DK3183340 T3 DK 3183340T3
Authority
DK
Denmark
Prior art keywords
termolabile
exonucleaser
termolabile exonucleaser
Prior art date
Application number
DK15770444.6T
Other languages
English (en)
Inventor
Marit Sjo Lorentzen
Yvonne Piotrowski
Nils Peder Willassen
Terese Solstad
Atle Noralf Larsen
Elisabeth Lill Andreassen
Morten Elde
Olav Lanes
Original Assignee
Arcticzymes As
Univ I Tromsoe Norges Arktiske Univ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arcticzymes As, Univ I Tromsoe Norges Arktiske Univ filed Critical Arcticzymes As
Application granted granted Critical
Publication of DK3183340T3 publication Critical patent/DK3183340T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/11Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11001Exodeoxyribonuclease I (3.1.11.1)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/30Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/325Single stranded exonuclease
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/916Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922Ribonucleases (RNAses); Deoxyribonucleases (DNAses)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK15770444.6T 2014-08-19 2015-08-19 Termolabile exonukleaser DK3183340T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1414745.8A GB201414745D0 (en) 2014-08-19 2014-08-19 Exonucleases
PCT/EP2015/001703 WO2016026574A1 (en) 2014-08-19 2015-08-19 Thermolabile exonucleases

Publications (1)

Publication Number Publication Date
DK3183340T3 true DK3183340T3 (da) 2019-08-19

Family

ID=51662672

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15770444.6T DK3183340T3 (da) 2014-08-19 2015-08-19 Termolabile exonukleaser

Country Status (12)

Country Link
US (3) US10087483B2 (da)
EP (1) EP3183340B1 (da)
JP (1) JP6316505B2 (da)
KR (2) KR20170040263A (da)
CN (1) CN107109383B (da)
CA (1) CA2956853C (da)
DK (1) DK3183340T3 (da)
ES (1) ES2742186T3 (da)
GB (1) GB201414745D0 (da)
LT (1) LT3183340T (da)
PL (1) PL3183340T3 (da)
WO (1) WO2016026574A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018013250A1 (en) * 2016-07-14 2018-01-18 Gene Infinity Llc Methods of rapid ligation-independent cloning of dna and uses thereof
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
GB201721053D0 (en) * 2017-12-15 2018-01-31 Univ I Tromsø - Norges Arktiske Univ DNA polymerases
WO2020172362A1 (en) * 2019-02-20 2020-08-27 Genetics Research, Llc Regulated multiplex reactions in a single tube
WO2021064106A1 (en) 2019-10-01 2021-04-08 Universitetet I Tromsø - Norges Arktiske Universitet Dna polymerase and dna polymerase derived 3'-5'exonuclease
CN114761548A (zh) * 2019-10-01 2022-07-15 特罗姆瑟大学-挪威北极圈大学 海洋dna聚合酶i
EP4200414A2 (en) * 2020-08-21 2023-06-28 Ultima Genomics, Inc. Compositions for surface amplification and uses thereof
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674679A (en) 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US5756285A (en) 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
CA2101119A1 (en) * 1992-08-17 1994-02-18 George T. Walker Exonuclease decontamination method
CA2137728C (en) 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
CA2168712A1 (en) 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
WO1997021837A1 (en) * 1995-12-15 1997-06-19 Amersham Life Science, Inc. Methods for the detection and removal of mutant sequences that arise during enzymatic amplification using mismatch repair systems
GB9918150D0 (en) 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
US6379940B2 (en) 2000-03-21 2002-04-30 Usb Corporation Stable composition comprising a nuclease and a phosphatase
US8361712B2 (en) 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
US20030087278A1 (en) 2001-08-15 2003-05-08 Jon Sayers Nuclease assay
EP1444354B1 (en) 2001-11-13 2007-01-31 Serono Genetics Institute S.A. Method for enhancing the efficiency of an enzymatic purification process
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
KR101038137B1 (ko) * 2002-06-28 2011-05-31 프리메라디엑스, 인크. 서열 차이를 감지하는 방법
JP3959315B2 (ja) 2002-07-26 2007-08-15 株式会社東芝 核酸汚染防止方法、それにおいて使用される抱埋化プライマーおよびそれを具備するキット
US20040126765A1 (en) 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
EP1704246A4 (en) 2003-09-30 2007-11-28 Perkinelmer Las Inc COMPOSITIONS AND METHODS FOR GENOTYPING POLYMORPHISMS OF A SINGLE NUCLEOTIDE
WO2006095769A1 (ja) 2005-03-08 2006-09-14 Takara Bio Inc. 低温性微生物由来エンドヌクレアーゼ
WO2007143206A2 (en) 2006-06-02 2007-12-13 Epicentre Technologies Compositions and methods for removal of dna from a sample
WO2009100080A1 (en) 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
US9249457B2 (en) * 2008-06-18 2016-02-02 Riken Method of manufacturing a mixture of amplified double-stranded nucleic acids comprising unknown sequence
WO2010045326A1 (en) 2008-10-14 2010-04-22 Barnes Wayne M In vitro recombination methods using 5' exonuclease
JP2012516145A (ja) 2009-01-30 2012-07-19 オックスフォード ナノポア テクノロジーズ リミテッド ハイブリダイゼーションリンカー
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
GB201120494D0 (en) 2011-11-29 2012-01-11 Univ Sheffield Modified exonuclease
AU2013220102A1 (en) 2012-02-17 2014-08-21 Biotec Pharmacon Asa Endonucleases

Also Published As

Publication number Publication date
US10087483B2 (en) 2018-10-02
PL3183340T3 (pl) 2019-12-31
US20170233800A1 (en) 2017-08-17
US10787702B2 (en) 2020-09-29
ES2742186T3 (es) 2020-02-13
KR20170040263A (ko) 2017-04-12
CA2956853A1 (en) 2016-02-25
US20200332352A9 (en) 2020-10-22
KR20180040736A (ko) 2018-04-20
CN107109383A (zh) 2017-08-29
EP3183340B1 (en) 2019-05-15
GB201414745D0 (en) 2014-10-01
JP2017523803A (ja) 2017-08-24
US20180363042A1 (en) 2018-12-20
CN107109383B (zh) 2019-04-02
JP6316505B2 (ja) 2018-04-25
US10415082B2 (en) 2019-09-17
KR102432501B1 (ko) 2022-08-12
US20200071753A1 (en) 2020-03-05
LT3183340T (lt) 2019-10-25
EP3183340A1 (en) 2017-06-28
CA2956853C (en) 2022-05-17
WO2016026574A1 (en) 2016-02-25

Similar Documents

Publication Publication Date Title
DK3134402T3 (da) 4-amino-imidazoquinolinforbindelser
DK3183051T3 (da) Væske-til-luftmembranenergivekslere
DK3211743T3 (da) Ø-til-netværk-omskiftningsfremgangsmåde
DK3222714T3 (da) Phytasemutanter
DK3119396T3 (da) Muscarinreceptoragonister
DK3006072T3 (da) Karadgang
DE112015001664A5 (de) Betätigungsaktuator
DK3183340T3 (da) Termolabile exonukleaser
DK3110439T3 (da) Pcsk9-vacciner
DK3154703T3 (da) Fuldkappe-snekkecentrifuge
FI20145040A (fi) Ajoneuvon rengas
DE112015005041A5 (de) Gargerätevorrichtung
DE102014000623A8 (de) Halbhohlnietelement
DK3009858T3 (da) Skyradar
DE112015001633A5 (de) Betätigungsaktuator
DK3447493T3 (da) Proteinrettede ortologer
ES1133830Y (es) Columbario
DK3091875T3 (da) Vipbar taburet
DE112015005055A5 (de) Gargerätevorrichtung
DK3191825T3 (da) Kreatininbiosensor
FI10894U1 (fi) Putkieristyskouru
ES1137381Y (es) Trompo
ES1136185Y (es) Huevera
FI10806U1 (fi) Ajoestepylväs
FI10861U1 (fi) Liukumisenestonasta