DK3133154T3 - Amylasepolypeptider - Google Patents
Amylasepolypeptider Download PDFInfo
- Publication number
- DK3133154T3 DK3133154T3 DK16181640.0T DK16181640T DK3133154T3 DK 3133154 T3 DK3133154 T3 DK 3133154T3 DK 16181640 T DK16181640 T DK 16181640T DK 3133154 T3 DK3133154 T3 DK 3133154T3
- Authority
- DK
- Denmark
- Prior art keywords
- gly
- asp
- ala
- ser
- val
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
Claims (14)
1. Polypeptid med amylaseaktivitet, der omfatter en aminosyresekvens med mindst 65 % sekvensidentitet med aminosyresekvensen ifølge SEQ ID NO: 1, og hvor polypeptidet omfatter en aminosyresubstitution ved følgende position: 88, med reference til positionsnummereringen fra sekvensen vist som SEQ ID NO: 1; hvor polypeptidet har forbedret termostabilitet sammenlignet med amylasen af SEQ ID NO: 1.
2. Polypeptid ifølge krav 1, hvor polypeptidet omfatter én eller flere aminosyresubstitutioner ved følgende positioner: 88, 205, 235, 240, 311, eller 409 og/eller én eller flere af følgende aminosyresubstitutioner: 42K/A/V/N/I/H/F, 34Q, 100Q/K/N/R, 272D eller 392K/D/E/Y/N/Q/R/S/T/G.
3. Polypeptid ifølge krav 1 eller 2, hvor polypeptidet endvidere omfatter aminosyren 223A/K/S.
4. Polypeptid ifølge et hvilket som helst af kravene 1-3 med mindst 90, 91, 92, 93, 94, 95, 96, 97, 98, eller 99 % sekvensidentitet med aminosyresekvensen ifølge SEQ ID NO: 1.
5. Polypeptid ifølge krav 1, der omfatter en aminosyresekvens valgt fra gruppen bestående af SEQ ID NO: 21.
6. Polypeptid ifølge et hvilket som helst af kravene 1-5 med en linker kondenseret ved C-terminus.
7. Polypeptid ifølge et hvilket som helst af kravene 1-6 med exoamylaseaktivitet, fortrinsvis ikke-maltogen exoamylaseaktivitet.
8. Nukleinsyre, der er i stand til at kode for et polypeptid ifølge et hvilket som helst af kravene 1-7.
9. Ekspressionsvektor, der omfatter en nukleinsyre ifølge krav 8, eller som er i stand til at udtrykke et polypeptid ifølge et hvilket som helst af kravene 1-7.
10. Plasmid, der omfatter en nukleinsyre ifølge krav 8.
11. Værtscelle, der omfatter, fortrinsvis transformeret med, en ekspressionsvektor ifølge krav 9 eller et plasmid ifølge krav 10.
12. Anvendelse af et polypeptid ifølge et hvilket som helst af kravene 1-7 som et fødevare- eller foderadditiv.
13. Fremgangsmåde til fremstilling af en saccharidsirup, hvilken fremgangsmåde omfatter tilsætning af et polypeptid ifølge et hvilket som helst af kravene 1-7 til en granuløs stivelseslikvefaktion for at danne saccharidsiroppen.
14. Fødevareprodukt, der omfatter et polypeptid ifølge et hvilket som helst af kravene 1-7.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17952509P | 2009-05-19 | 2009-05-19 | |
| EP09160655 | 2009-05-19 | ||
| EP10723554.1A EP2432875B1 (en) | 2009-05-19 | 2010-05-19 | Amylase polypeptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3133154T3 true DK3133154T3 (da) | 2019-01-28 |
Family
ID=41258492
Family Applications (8)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18196821.5T DK3473711T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
| DK18180444.4T DK3412771T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
| DK19190067.9T DK3591046T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptide |
| DK18153826.5T DK3346004T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
| DK20198751.8T DK3783103T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
| DK19201468.6T DK3620518T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptid |
| DK10723554.1T DK2432875T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptide |
| DK16181640.0T DK3133154T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
Family Applications Before (7)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18196821.5T DK3473711T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
| DK18180444.4T DK3412771T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
| DK19190067.9T DK3591046T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptide |
| DK18153826.5T DK3346004T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
| DK20198751.8T DK3783103T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptider |
| DK19201468.6T DK3620518T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptid |
| DK10723554.1T DK2432875T3 (da) | 2009-05-19 | 2010-05-19 | Amylasepolypeptide |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8753859B2 (da) |
| EP (8) | EP3783103B1 (da) |
| CN (1) | CN102428176B (da) |
| AU (1) | AU2010251109B2 (da) |
| BR (1) | BRPI1012787A8 (da) |
| DK (8) | DK3473711T3 (da) |
| ES (4) | ES2604666T3 (da) |
| MX (1) | MX2011011911A (da) |
| WO (1) | WO2010133644A2 (da) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2486799A1 (en) * | 2011-02-14 | 2012-08-15 | DSM IP Assets B.V. | Method to produce cake with lipolytic enzyme and alpha-amylase |
| MX2014007603A (es) * | 2011-12-26 | 2014-09-12 | Dupont Nutrition Biosci Aps | Uso de enzima exoamilasa. |
| US11692016B2 (en) | 2012-03-09 | 2023-07-04 | Vestaron Corporation | High gene expression yeast strain |
| CN112400900A (zh) | 2012-03-09 | 2021-02-26 | 韦斯塔隆公司 | 毒肽产生、植物中的肽表达和富含半胱氨酸的肽的组合 |
| US20150373999A1 (en) * | 2013-03-01 | 2015-12-31 | Dsm Ip Assets B.V. | Combination of an alpha-amylase and a g4-forming amylase |
| WO2015086027A1 (en) | 2013-12-09 | 2015-06-18 | Carlsberg A/S | Stable haze for beverages |
| CN103695386B (zh) * | 2013-12-25 | 2015-09-16 | 南宁邦尔克生物技术有限责任公司 | 一种耐酸性高温β-淀粉酶突变体及其应用 |
| CN105219753B (zh) * | 2015-11-03 | 2018-11-02 | 绍兴加华生物科技有限公司 | 一种固定化的有机磷农药降解酶及其制备方法与应用 |
| WO2017152169A1 (en) | 2016-03-04 | 2017-09-08 | Danisco Us Inc. | Engineered ribosomal promoters for protein production in microorganisms |
| CN109072204A (zh) * | 2016-04-29 | 2018-12-21 | 焙乐道有限责任公司 | 改善的烘焙制品 |
| CN110267527A (zh) * | 2016-10-21 | 2019-09-20 | 韦斯塔隆公司 | 可切割肽及包含可切割肽的杀昆虫和杀线虫蛋白 |
| US11866713B2 (en) | 2017-02-24 | 2024-01-09 | Danisco Us Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
| WO2019089898A1 (en) | 2017-11-02 | 2019-05-09 | Danisco Us Inc | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
| WO2020185737A1 (en) * | 2019-03-11 | 2020-09-17 | Basf Se | Amylases and methods for making and using them |
| US20230172214A1 (en) | 2020-04-30 | 2023-06-08 | Dupont Nutrition Biosciences Aps | Ingredient system for bakery products |
| EP4071242A1 (en) * | 2021-04-06 | 2022-10-12 | DuPont Nutrition Biosciences ApS | Amylase polypeptides with improved properties |
| WO2024088550A1 (en) | 2022-10-24 | 2024-05-02 | Novozymes A/S | Baking method for pulse protein fortified bread employing thermostable amyloglucosidase variante (ec 3.2.1.3) |
| WO2024088549A1 (en) | 2022-10-24 | 2024-05-02 | Novozymes A/S | Baking method with thermostable amg variant and alpha-amylase |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK135983D0 (da) | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
| DK154572C (da) | 1985-08-07 | 1989-04-24 | Novo Industri As | Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler |
| EP0625577A1 (en) | 1985-08-29 | 1994-11-23 | Genencor International, Inc. | Heterologous polypeptides expressed in filamentous fungi, processes for their preparation, and vectors for their preparation |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| GB8610600D0 (en) | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| WO1988002775A1 (en) | 1986-10-17 | 1988-04-21 | Novo Industri A/S | Positionally non-specific lipase from candida sp, a method for producing it, its use and a recombinant dna process for producing it |
| DK399387D0 (da) | 1987-07-31 | 1987-07-31 | Novo Industri As | Immobiliseret lipase og dennes anvendelse |
| EP0305216B1 (en) | 1987-08-28 | 1995-08-02 | Novo Nordisk A/S | Recombinant Humicola lipase and process for the production of recombinant humicola lipases |
| DK474589D0 (da) | 1989-09-27 | 1989-09-27 | Novo Nordisk As | Fremgangsmaade til fremstilling af bageriprodukter |
| DE4017150A1 (de) | 1990-05-28 | 1991-12-05 | Henkel Kgaa | Verfahren zur herstellung eines backaktiven pentosanase-praeparates |
| NL9001388A (nl) | 1990-06-19 | 1992-01-16 | Unilever Nv | Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan. |
| US5162210A (en) | 1990-06-29 | 1992-11-10 | Iowa State University Research Foundation | Process for enzymatic hydrolysis of starch to glucose |
| IE20040144A1 (en) | 1990-07-24 | 2004-05-19 | Dsm Nv | Cloning and expression of xylanase genes from fungal origin |
| WO1992010581A1 (en) | 1990-12-10 | 1992-06-25 | Genencor International, Inc. | IMPROVED SACCHARIFICATION OF CELLULOSE BY CLONING AND AMPLIFICATION OF THE β-GLUCOSIDASE GENE OF TRICHODERMA REESEI |
| KR100234888B1 (ko) | 1991-04-02 | 1999-12-15 | 한센 핀 베네드, 안네 제헤르 | 크실라나제, 대응하는 재조합 dna 서열, 크실라나제 함유 조성물, 및 조성물의 용도 |
| DK76893D0 (da) | 1993-06-28 | 1993-06-28 | Novo Nordisk As | |
| DK114893D0 (da) | 1993-10-14 | 1993-10-14 | Novo Nordisk As | |
| US5861271A (en) | 1993-12-17 | 1999-01-19 | Fowler; Timothy | Cellulase enzymes and systems for their expressions |
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| EP1068302B1 (en) | 1998-04-01 | 2005-06-01 | Danisco A/S | Non-maltogenic exoamylases and their use in retarding retrogradation of starch |
| US6268328B1 (en) | 1998-12-18 | 2001-07-31 | Genencor International, Inc. | Variant EGIII-like cellulase compositions |
| CA3032990C (en) * | 2001-02-21 | 2020-07-14 | Basf Enzymes Llc | Enzymes having alpha amylase activity and methods of use thereof |
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| DE602004026733D1 (de) | 2003-06-13 | 2010-06-02 | Danisco | Pseudomonas-polypeptidvarianten mit einer nicht maltogenen exoamylaseaktivität und ihre verwendung bei der herstellung von lebensmittelprodukten |
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| US8143048B2 (en) | 2003-07-07 | 2012-03-27 | Danisco A/S | Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof |
| US20060018997A1 (en) | 2004-07-07 | 2006-01-26 | Kragh Karsten M | Polypeptide |
| US20060073583A1 (en) | 2004-09-22 | 2006-04-06 | Kragh Karsten M | Polypeptide |
| US20060008890A1 (en) | 2004-07-07 | 2006-01-12 | Kragh Karsten M | Polypeptide |
| WO2006003461A2 (en) * | 2004-07-07 | 2006-01-12 | Danisco A/S | Polypeptide |
| US20060008888A1 (en) | 2004-07-07 | 2006-01-12 | Kragh Karsten M | Polypeptide |
| KR20070039593A (ko) | 2004-07-21 | 2007-04-12 | 암브룩스, 인코포레이티드 | 비천연적으로 코딩된 아미노산을 이용한 생합성 폴리펩티드 |
| CA2614274A1 (en) | 2005-07-07 | 2007-01-18 | Danisco A/S | Modified amylase from pseudomonas saccharophilia |
| US8030050B2 (en) * | 2005-07-07 | 2011-10-04 | Danisco A/S | Modified amylases from Pseudomonas species |
| MX2008016444A (es) * | 2006-06-19 | 2009-01-22 | Danisco | Polipeptido. |
| EP2215202B2 (en) * | 2007-11-05 | 2024-01-10 | Danisco US Inc. | VARIANTS OF BACILLUS sp. TS-23 ALPHA-AMYLASE WITH ALTERED PROPERTIES |
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2010
- 2010-05-19 DK DK18196821.5T patent/DK3473711T3/da active
- 2010-05-19 DK DK18180444.4T patent/DK3412771T3/da active
- 2010-05-19 DK DK19190067.9T patent/DK3591046T3/da active
- 2010-05-19 EP EP20198751.8A patent/EP3783103B1/en active Active
- 2010-05-19 EP EP16181640.0A patent/EP3133154B1/en active Active
- 2010-05-19 EP EP18180444.4A patent/EP3412771B1/en active Active
- 2010-05-19 AU AU2010251109A patent/AU2010251109B2/en not_active Ceased
- 2010-05-19 EP EP19201468.6A patent/EP3620518B1/en active Active
- 2010-05-19 ES ES10723554.1T patent/ES2604666T3/es active Active
- 2010-05-19 CN CN201080021717.4A patent/CN102428176B/zh active Active
- 2010-05-19 DK DK18153826.5T patent/DK3346004T3/da active
- 2010-05-19 EP EP19190067.9A patent/EP3591046B1/en active Active
- 2010-05-19 EP EP18196821.5A patent/EP3473711B1/en active Active
- 2010-05-19 ES ES19201468T patent/ES2897742T3/es active Active
- 2010-05-19 DK DK20198751.8T patent/DK3783103T3/da active
- 2010-05-19 DK DK19201468.6T patent/DK3620518T3/da active
- 2010-05-19 EP EP18153826.5A patent/EP3346004B1/en active Active
- 2010-05-19 DK DK10723554.1T patent/DK2432875T3/da active
- 2010-05-19 ES ES19190067T patent/ES2882615T3/es active Active
- 2010-05-19 BR BRPI1012787A patent/BRPI1012787A8/pt not_active Application Discontinuation
- 2010-05-19 ES ES20198751T patent/ES2923524T3/es active Active
- 2010-05-19 DK DK16181640.0T patent/DK3133154T3/da active
- 2010-05-19 MX MX2011011911A patent/MX2011011911A/es active IP Right Grant
- 2010-05-19 EP EP10723554.1A patent/EP2432875B1/en active Active
- 2010-05-19 WO PCT/EP2010/056913 patent/WO2010133644A2/en active Application Filing
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2011
- 2011-11-02 US US13/287,492 patent/US8753859B2/en active Active
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