DK3023777T3 - Fremgangsmåde til fremstilling af peptidfragmenter, kit til fremstilling af peptidfragmenter til anvendelse deri, samt analysefremgangsmåde - Google Patents
Fremgangsmåde til fremstilling af peptidfragmenter, kit til fremstilling af peptidfragmenter til anvendelse deri, samt analysefremgangsmåde Download PDFInfo
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- DK3023777T3 DK3023777T3 DK13893016.9T DK13893016T DK3023777T3 DK 3023777 T3 DK3023777 T3 DK 3023777T3 DK 13893016 T DK13893016 T DK 13893016T DK 3023777 T3 DK3023777 T3 DK 3023777T3
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- DK
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- Prior art keywords
- protease
- antibody
- peptide fragments
- immobilized
- porous body
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
- G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/976—Trypsin; Chymotrypsin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Computational Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Optics & Photonics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Claims (14)
1. Fremgangsmåde til fremstilling af peptidfragmenter, omfattende: et substratimmobiliseringstrin med immobilisering af et substratprotein, som skal pro-teolyseres, i porer i et porøst legeme; og et proteolysetrin med proteolysering af substratproteinet med en protease ved at bringe det porøse legeme med substratproteinet immobiliseret derpå og mikropartikler med proteasen immobiliseret på overfladen deraf i kontakt med hinanden i en væske, hvor en gennemsnitlig partikeldiameter for mikropartiklerne er større end en gennemsnitlig porediameter for det porøse legeme.
2. Fremgangsmåde til fremstilling af peptidfragmenter ifølge krav 1, hvor, i substratimmobiliseringstrinnet, en forudbestemt region af substratproteinet immobiliseres på det porøse legeme, og en region af substratproteinet afvigende fra den forudbestemte region underkastes en positionsselektiv proteaseproteolyse.
3. Fremgangsmåde til fremstilling af peptidfragmenter ifølge krav 1 eller 2, hvor et linker-molekyle, som er i stand til positionsspecifik interaktion med substratproteinet, er immobiliseret i porerne i det porøse legeme, og i substratimmobiliseringstrinnet immobiliseres substratproteinet i porerne af det porøse legeme via linkermolekylet.
4. Fremgangsmåde til fremstilling af peptidfragmenter ifølge krav 3, hvor, efter substratimmobiliseringstrinnet, et molekyle, i hvilket linkermolekylet binder med substratproteinet, har en størrelse på 0,5 gange til 1,5 gange porediameteren i det porøse legeme.
5. Fremgangsmåde til fremstilling af peptidfragmenter ifølge ethvert af kravene 1 til 4, hvor overfladen på mikropartiklerne er modificeret med et afstandsmolekyle, som er i stand til at binde med proteasen, og proteasen immobiliseres på overfladen af mikropartiklerne via afstandsmolekylet.
6. Fremgangsmåde til fremstilling af peptidfragmenter ifølge ethvert af kravene 1 til 5, hvor substratproteinet er et antistof.
7. Fremgangsmåde til fremstilling af peptidfragmenter ifølge krav 6, hvor antistoffet er et monoklonalt antistof.
8. Fremgangsmåde til fremstilling af peptidfragmenter ifølge krav 6 eller 7, hvor, i substratimmobiliseringstrinnet, et Fc domæne af antistoffet immobiliseres på det porøse legeme, og i proteolysetrinnet bliver et Fab domæne af antistoffet positionsselektivt proteolyseret af proteasen.
9. Fremgangsmåde til fremstilling af peptidfragmenter ifølge ethvert af kravene 1 til 8, hvor proteasen er trypsin.
10. Fremgangsmåde til fremstilling af peptidfragmenter ifølge ethvert af kravene 1 til 9, hvor den gennemsnitlige porediameter for det porøse legeme er 30 til 150 nm og den gennemsnitlige partikeldiameter for mikropartiklerne er 100 nm eller mere.
11. Sæt til fremstilling af peptidfragmenter til anvendelse ved fremgangsmåden ifølge ethvert af kravene 1 til 10, hvilket sæt omfatter: et porøst legeme med porer, som er i stand til at immobilisere et substratprotein, og mikropartikler, som er i stand til at immobilisere en protease på overfladen deraf, hvor en gennemsnitlig partikeldiameter for mikropartiklerne er større end en porediameter for det porøse legeme.
12. Sæt til fremstilling af peptidfragmenter ifølge krav 11, hvor mikropartiklerne er tilvejebragt i en tilstand, hvor proteasen er immobiliseret på overfladen deraf.
13. Analysefremgangsmåde omfattende fremstillingen af peptidfragmenter ifølge en fremgangsmåde ifølge ethvert af kravene 1 til 10 og analyse ved massespektrometri.
14. Analysefremgangsmåde ifølge krav 13, hvor peptidfragmenterne, som skal analyseres, indeholder i det mindste en del af en aminosyresekvens af en komplementaritetsbestemmende region af et antistof.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2013/074292 WO2015033479A1 (ja) | 2013-09-09 | 2013-09-09 | ペプチド断片の調製方法およびそれに用いられるペプチド断片調製用キット、ならびに分析方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3023777T3 true DK3023777T3 (da) | 2018-01-15 |
Family
ID=52627984
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK13893016.9T DK3023777T3 (da) | 2013-09-09 | 2013-09-09 | Fremgangsmåde til fremstilling af peptidfragmenter, kit til fremstilling af peptidfragmenter til anvendelse deri, samt analysefremgangsmåde |
Country Status (14)
Country | Link |
---|---|
US (3) | US10539571B2 (da) |
EP (1) | EP3023777B1 (da) |
JP (1) | JP5924455B2 (da) |
KR (1) | KR101826449B1 (da) |
CN (1) | CN105518447B (da) |
CA (1) | CA2922372C (da) |
DK (1) | DK3023777T3 (da) |
ES (1) | ES2649147T3 (da) |
NO (1) | NO3023777T3 (da) |
PL (1) | PL3023777T3 (da) |
PT (1) | PT3023777T (da) |
SG (1) | SG11201601031QA (da) |
TW (1) | TWI551687B (da) |
WO (1) | WO2015033479A1 (da) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105518447B (zh) | 2013-09-09 | 2018-01-16 | 株式会社岛津制作所 | 肽片段的制备方法及该方法中使用的肽片段制备用试剂盒、以及分析方法 |
CN107407666A (zh) | 2015-03-09 | 2017-11-28 | 株式会社岛津制作所 | 使用质谱分析的单克隆抗体的检测方法 |
CN107407665A (zh) * | 2015-03-10 | 2017-11-28 | 株式会社岛津制作所 | 用于检测单克隆抗体的样品调制用试剂盒 |
WO2016194114A1 (ja) * | 2015-06-01 | 2016-12-08 | 株式会社島津製作所 | モノクローナル抗体の定量方法 |
SG11201800894SA (en) * | 2015-08-03 | 2018-03-28 | Shimadzu Corp | Method for parallel quantification of protein variant |
EP3480577A4 (en) * | 2016-06-30 | 2020-07-29 | Shimadzu Corporation | SET OF CONTAINERS AND SAMPLE PREPARATION PROCESS USING IT |
WO2018025346A1 (ja) * | 2016-08-03 | 2018-02-08 | 株式会社島津製作所 | ペプチド断片の調製方法およびそれに用いられるプロテアーゼの調製方法、ならびにペプチド断片調製用キット |
WO2018167847A1 (ja) * | 2017-03-14 | 2018-09-20 | 株式会社島津製作所 | モノクローナル抗体の同時定量方法 |
JP6984657B2 (ja) | 2017-06-22 | 2021-12-22 | 株式会社島津製作所 | 抗原または抗抗体が結合したモノクローナル抗体の定量方法 |
US11085856B2 (en) | 2017-09-15 | 2021-08-10 | Thermo Finnigan Llc | Method and product for preparing a protein-containing sample for analysis by mass spectrometry |
CN111512152B (zh) * | 2017-12-28 | 2023-08-11 | 株式会社岛津制作所 | 改善单克隆抗体的检测结果的方法 |
JP6835260B2 (ja) * | 2017-12-28 | 2021-02-24 | 株式会社島津製作所 | モノクローナル抗体の簡素化された定量方法 |
US20200378986A1 (en) * | 2018-05-24 | 2020-12-03 | Sigma-Aldrich Co. Llc | Quantitative analysis of proteins |
GB2607197B (en) * | 2018-06-06 | 2023-04-26 | Bruker Daltonics Gmbh & Co Kg | Targeted protein characterization by mass spectrometry |
WO2022270126A1 (ja) | 2021-06-23 | 2022-12-29 | 株式会社島津製作所 | 抗体を分析する方法 |
Family Cites Families (19)
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US4071409A (en) * | 1976-05-20 | 1978-01-31 | Corning Glass Works | Immobilization of proteins on inorganic support materials |
GB8902770D0 (en) * | 1989-02-08 | 1989-03-30 | Bioprocessing Ltd | Improvements in or relating to supports for immunoaffinity separations |
GB0006046D0 (en) * | 2000-03-13 | 2000-05-03 | Univ Warwick | Time of flight mass spectrometry apparatus |
US6916423B2 (en) | 2000-07-18 | 2005-07-12 | Invitrogen Corporation | Device and methods for subdividing and filtering gel material and extracting molecules therefrom |
EP1495323A2 (en) * | 2002-04-05 | 2005-01-12 | University Of Georgia Research Foundation, Inc. | Method for cleaving and deglycosylating antibodies to promote ligand binding |
DE102004021351A1 (de) * | 2004-04-23 | 2005-11-17 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Funktionalisierter poröser Träger für Mikroarrays |
US7258990B2 (en) * | 2004-08-19 | 2007-08-21 | Biocept, Inc. | Alleviation of non-specific binding in microarray assays |
US9138467B2 (en) * | 2005-01-28 | 2015-09-22 | Stipkovits, Laszlo, Dr. | Immunologically active compositions |
CA2499657A1 (en) * | 2005-03-03 | 2006-09-03 | Richard Oleschuk | Polymer entrapped particles |
JP2009516172A (ja) * | 2005-11-10 | 2009-04-16 | ロゼッタ インファーマティックス エルエルシー | 合成画像を使用した生物学的特徴の発見 |
PT2617823E (pt) * | 2006-09-21 | 2015-11-23 | Basf Enzymes Llc | Fitases, ácidos nucleicos que as codificam e métodos para as preparar e utilizar |
US20100015652A1 (en) * | 2006-12-21 | 2010-01-21 | Brian Walter Granda | Antibody quantitation |
US8074494B2 (en) * | 2007-12-10 | 2011-12-13 | Proxeon Biosystems A/S | Plugged flow 2D chromatography |
US8592555B2 (en) * | 2008-08-11 | 2013-11-26 | Emd Millipore Corporation | Immunoglobulin-binding proteins with improved specificity |
JP2011130749A (ja) | 2009-12-25 | 2011-07-07 | Bio Matrix Research Inc | 抗体のフラグメント化促進剤 |
CN108220297A (zh) * | 2011-03-09 | 2018-06-29 | 细胞信号科技公司 | 用于生成单克隆抗体的方法和试剂 |
CN105518447B (zh) * | 2013-09-09 | 2018-01-16 | 株式会社岛津制作所 | 肽片段的制备方法及该方法中使用的肽片段制备用试剂盒、以及分析方法 |
CN107407666A (zh) * | 2015-03-09 | 2017-11-28 | 株式会社岛津制作所 | 使用质谱分析的单克隆抗体的检测方法 |
JP6835260B2 (ja) * | 2017-12-28 | 2021-02-24 | 株式会社島津製作所 | モノクローナル抗体の簡素化された定量方法 |
-
2013
- 2013-09-09 CN CN201380078545.8A patent/CN105518447B/zh active Active
- 2013-09-09 PT PT138930169T patent/PT3023777T/pt unknown
- 2013-09-09 KR KR1020167004300A patent/KR101826449B1/ko active IP Right Grant
- 2013-09-09 NO NO13893016A patent/NO3023777T3/no unknown
- 2013-09-09 JP JP2015535281A patent/JP5924455B2/ja active Active
- 2013-09-09 CA CA2922372A patent/CA2922372C/en not_active Expired - Fee Related
- 2013-09-09 PL PL13893016T patent/PL3023777T3/pl unknown
- 2013-09-09 DK DK13893016.9T patent/DK3023777T3/da active
- 2013-09-09 WO PCT/JP2013/074292 patent/WO2015033479A1/ja active Application Filing
- 2013-09-09 EP EP13893016.9A patent/EP3023777B1/en active Active
- 2013-09-09 SG SG11201601031QA patent/SG11201601031QA/en unknown
- 2013-09-09 ES ES13893016.9T patent/ES2649147T3/es active Active
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2014
- 2014-06-10 TW TW103119967A patent/TWI551687B/zh not_active IP Right Cessation
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2016
- 2016-03-08 US US15/063,774 patent/US10539571B2/en active Active
-
2019
- 2019-11-14 US US16/683,560 patent/US11022616B2/en active Active
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2021
- 2021-04-15 US US17/231,141 patent/US11650210B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JPWO2015033479A1 (ja) | 2017-03-02 |
SG11201601031QA (en) | 2016-03-30 |
US10539571B2 (en) | 2020-01-21 |
NO3023777T3 (da) | 2018-04-14 |
TW201510222A (zh) | 2015-03-16 |
JP5924455B2 (ja) | 2016-05-25 |
WO2015033479A1 (ja) | 2015-03-12 |
US20160252522A1 (en) | 2016-09-01 |
KR101826449B1 (ko) | 2018-02-06 |
CA2922372A1 (en) | 2015-03-12 |
EP3023777A1 (en) | 2016-05-25 |
ES2649147T3 (es) | 2018-01-10 |
CA2922372C (en) | 2018-05-15 |
US11650210B2 (en) | 2023-05-16 |
US11022616B2 (en) | 2021-06-01 |
EP3023777B1 (en) | 2017-11-15 |
PL3023777T3 (pl) | 2018-04-30 |
TWI551687B (zh) | 2016-10-01 |
KR20160031550A (ko) | 2016-03-22 |
CN105518447B (zh) | 2018-01-16 |
EP3023777A4 (en) | 2016-09-21 |
CN105518447A (zh) | 2016-04-20 |
US20210263044A1 (en) | 2021-08-26 |
PT3023777T (pt) | 2018-01-19 |
US20200072846A1 (en) | 2020-03-05 |
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