DK2970915T3 - Fremgangsmåder og sammensætninger til generering af stabile transficerede celler - Google Patents
Fremgangsmåder og sammensætninger til generering af stabile transficerede celler Download PDFInfo
- Publication number
- DK2970915T3 DK2970915T3 DK14763369T DK14763369T DK2970915T3 DK 2970915 T3 DK2970915 T3 DK 2970915T3 DK 14763369 T DK14763369 T DK 14763369T DK 14763369 T DK14763369 T DK 14763369T DK 2970915 T3 DK2970915 T3 DK 2970915T3
- Authority
- DK
- Denmark
- Prior art keywords
- compositions
- methods
- transfected cells
- stable transfected
- generating stable
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
- C12Y207/01095—Kanamycin kinase (2.7.1.95), i.e. neomycin-kanamycin phosphotransferase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361794785P | 2013-03-15 | 2013-03-15 | |
| PCT/US2014/028561 WO2014144237A1 (en) | 2013-03-15 | 2014-03-14 | Methods and compositions for generating stable transfected cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2970915T3 true DK2970915T3 (da) | 2019-11-11 |
Family
ID=51537653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14763369T DK2970915T3 (da) | 2013-03-15 | 2014-03-14 | Fremgangsmåder og sammensætninger til generering af stabile transficerede celler |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10316335B2 (da) |
| EP (1) | EP2970915B1 (da) |
| JP (2) | JP6683598B2 (da) |
| CN (1) | CN105378071B (da) |
| DK (1) | DK2970915T3 (da) |
| WO (1) | WO2014144237A1 (da) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10316335B2 (en) | 2013-03-15 | 2019-06-11 | Maxcyte, Inc. | Methods and compositions for generating stable transfected cells |
| CN106687585B (zh) * | 2014-04-14 | 2021-11-02 | 美克斯细胞有限公司 | 用于修饰基因组dna的方法和组合物 |
| CA2982759A1 (en) | 2015-04-13 | 2016-10-20 | Maxcyte, Inc. | Methods and compositions for modifying genomic dna |
| CN110066768A (zh) * | 2018-08-24 | 2019-07-30 | 中国疾病预防控制中心营养与健康所 | 表达hFcεRIα的RBL-2H3细胞株的构建方法 |
| KR102129681B1 (ko) * | 2018-10-22 | 2020-07-02 | 부경대학교 산학협력단 | 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법 |
| CN112662612A (zh) * | 2020-12-30 | 2021-04-16 | 武汉北度生物科技有限公司 | 一种人体细胞外基质培育用筛选方法 |
| WO2023150797A2 (en) * | 2022-02-07 | 2023-08-10 | Stiles Brendon M | Targeting art1 for cancer immunotherapy |
| CN116478913B (zh) * | 2023-06-21 | 2023-08-18 | 苏州依科赛生物科技股份有限公司 | 一种cho细胞培养基及其应用 |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4554101A (en) | 1981-01-09 | 1985-11-19 | New York Blood Center, Inc. | Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity |
| DK0533838T3 (da) | 1990-06-11 | 1998-02-23 | Nexstar Pharmaceuticals Inc | Nukleinsyreligander |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| ATE272311T1 (de) | 1993-03-23 | 2004-08-15 | Cbr Lab Inc | Verfahren und vorrichtung zur einkapselung biologisch aktiver substanzen in zellen |
| JPH0844143A (ja) | 1993-06-29 | 1996-02-16 | Toshiba Corp | 帯電装置並びに該帯電装置を備えた画像形成装置 |
| KR19980702848A (ko) | 1995-03-10 | 1998-08-05 | 존 더블유 홀라데이 | 유동 일렉트로포레이션 체임버 및 방법 |
| US6074605A (en) | 1995-03-10 | 2000-06-13 | Entremed, Inc. | Flow electroporation chamber and method |
| US6773669B1 (en) | 1995-03-10 | 2004-08-10 | Maxcyte, Inc. | Flow electroporation chamber and method |
| US5720921A (en) | 1995-03-10 | 1998-02-24 | Entremed, Inc. | Flow electroporation chamber and method |
| US7067318B2 (en) | 1995-06-07 | 2006-06-27 | The Regents Of The University Of Michigan | Methods for transfecting T cells |
| JP3023740U (ja) * | 1995-06-23 | 1996-04-30 | 稲村印刷株式会社 | ノートの表紙等の抗菌性印刷物 |
| US6090617A (en) | 1996-12-05 | 2000-07-18 | Entremed, Inc. | Flow electroporation chamber with electrodes having a crystalline metal nitride coating |
| EP0972445B1 (en) * | 1997-02-28 | 2006-07-05 | Kirin Beer Kabushiki Kaisha | Chimeric mice that express a human antibody |
| WO2002022834A2 (en) | 2000-09-15 | 2002-03-21 | Deltagen, Inc. | Methods of producing cells and animals comprising targeted gene modifications |
| AU2001290147A1 (en) | 2000-09-29 | 2002-04-08 | Ecole Polytechnique Federale De Lausanne | Transfection using plasmid preparations |
| US7029916B2 (en) | 2001-02-21 | 2006-04-18 | Maxcyte, Inc. | Apparatus and method for flow electroporation of biological samples |
| WO2003018751A2 (en) * | 2001-08-22 | 2003-03-06 | Maxcyte, Inc. | Apparatus and method for electroporation of biological samples |
| AU2003277109A1 (en) | 2002-09-30 | 2004-04-23 | Maxcyte, Inc. | Apparatus and method for streaming electroporation |
| US20040214333A1 (en) | 2003-02-18 | 2004-10-28 | Maxcyte, Inc. | Loading of cells with antigens by electroporation |
| US7489362B2 (en) * | 2003-03-04 | 2009-02-10 | Broadcom Corporation | Television functionality on a chip |
| CA2565316C (en) | 2004-05-12 | 2017-05-30 | Maxcyte, Inc. | Methods and devices related to a regulated flow electroporation chamber |
| US7531327B2 (en) * | 2004-07-23 | 2009-05-12 | Immunomedics, Inc. | Methods and compositions for increasing longevity and protein yield from a cell culture |
| EP1802646B1 (en) | 2004-07-23 | 2017-09-06 | Immunomedics Inc. | Methods and compositions for increasing longevity and protein yield from a cell culture |
| US20070059833A1 (en) | 2005-09-07 | 2007-03-15 | Maxcyte, Inc. | Use of Nucleases to Improve Viability and Enhance Transgene Expression in Transfected Cells |
| EP2010668A4 (en) | 2006-04-10 | 2009-04-29 | Univ California | SYSTEMS AND METHODS FOR EFFICIENT COLLECTION OF UNIQUE CELLS AND COLONIES OF CELLS AND RAPID FORMATION OF STABLE TRANSFECTORS |
| WO2008153742A2 (en) | 2007-05-23 | 2008-12-18 | Sangamo Biosciences, Inc. | Methods and compositions for increased transgene expression |
| KR101009336B1 (ko) | 2008-12-31 | 2011-01-19 | 주식회사 하이닉스반도체 | 반도체 메모리 장치 및 그 구동 방법 |
| JP6039552B2 (ja) * | 2010-07-01 | 2016-12-07 | グラクソ グループ リミテッドＧｌａｘｏ Ｇｒｏｕｐ Ｌｉｍｉｔｅｄ | 高産生細胞株を選択するための改良された方法 |
| BR112013001685B1 (pt) | 2010-07-23 | 2021-10-13 | Sigma-Aldrich Co. Llc | Método para editar pelo menos uma sequência cromossômica endógena em uma célula pela inserção de uma sequência na sequência cromossômica |
| WO2013139994A1 (en) | 2012-03-23 | 2013-09-26 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | A novel method of producing an oocyte carrying a modified target sequence in its genome |
| KR102116153B1 (ko) | 2012-05-07 | 2020-05-27 | 상가모 테라퓨틱스, 인코포레이티드 | 도입유전자의 뉴클레아제-매개 표적화된 통합을 위한 방법 및 조성물 |
| CN102839214A (zh) | 2012-09-06 | 2012-12-26 | 许汉鹏 | 筛选真核细胞基因组内特定基因调控相关机制和分子的方法 |
| JP3181613U (ja) * | 2012-12-04 | 2013-02-14 | はまや印刷株式会社 | 書類フォルダ |
| US10316335B2 (en) | 2013-03-15 | 2019-06-11 | Maxcyte, Inc. | Methods and compositions for generating stable transfected cells |
| CN106687585B (zh) | 2014-04-14 | 2021-11-02 | 美克斯细胞有限公司 | 用于修饰基因组dna的方法和组合物 |
| JP3195272U (ja) * | 2014-07-07 | 2015-01-15 | 大同紙工印刷株式会社 | 香りつきマスク収納ケース |
| JP3222103U (ja) * | 2019-03-27 | 2019-07-11 | 有限会社 東宝ラベル | マスクケース |
-
2014
- 2014-03-14 US US14/777,079 patent/US10316335B2/en active Active
- 2014-03-14 EP EP14763369.7A patent/EP2970915B1/en active Active
- 2014-03-14 JP JP2016502834A patent/JP6683598B2/ja active Active
- 2014-03-14 DK DK14763369T patent/DK2970915T3/da active
- 2014-03-14 WO PCT/US2014/028561 patent/WO2014144237A1/en active Application Filing
- 2014-03-14 CN CN201480021503.5A patent/CN105378071B/zh active Active
-
2020
- 2020-01-09 JP JP2020001778A patent/JP2020072719A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2970915A4 (en) | 2016-10-26 |
| US10316335B2 (en) | 2019-06-11 |
| JP2016514454A (ja) | 2016-05-23 |
| JP6683598B2 (ja) | 2020-04-22 |
| JP2020072719A (ja) | 2020-05-14 |
| EP2970915B1 (en) | 2019-08-14 |
| US20160053283A1 (en) | 2016-02-25 |
| CN105378071B (zh) | 2021-04-20 |
| CN105378071A (zh) | 2016-03-02 |
| WO2014144237A1 (en) | 2014-09-18 |
| EP2970915A1 (en) | 2016-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3527068T3 (da) | Fremgangsmåder og sammensætninger til forøgelse af effektivitet af målrettet genmodifikation under anvendelse af oligonukleotid-medieret genreparation | |
| DK2978419T3 (da) | Sammensætninger til forbedring af cellelevedygtighed og fremgangsmåder til anvendelse deraf | |
| DK2991646T3 (da) | Fremgangsmåder og sammensætninger til forøgelse af cd4+-regulatoriske t-celler | |
| DK3556869T3 (da) | Sammensætninger og fremgangsmåder til sekventering af polynukleotider | |
| DK2970915T3 (da) | Fremgangsmåder og sammensætninger til generering af stabile transficerede celler | |
| DK2970456T3 (da) | Fremgangsmåder og sammensætninger til levering af mrna-kodede antistoffer | |
| DK3008168T3 (da) | Sc-celler og sammensætninger og fremgangsmåde til dannelse deraf | |
| DK3013964T3 (da) | Sammensætninger til indføring af rna i celler | |
| DK3137605T3 (da) | Sammensætninger og fremgangsmåder til modulering af angiopoietin-lignende-3-ekspression | |
| DK2991656T3 (da) | Sammensætninger og fremgangsmåder til modulering af apolipoprotein c-iii-ekspression | |
| DK2997132T3 (da) | Fremgangsmåder til konstruering af allogenisk og høj-aktiv T-celle til immunoterapi | |
| DK2970423T3 (da) | Interleukin-2 muteiner for udvidelsen af t-regulatoriske celler | |
| DK3219707T3 (da) | Fremgangsmåde til fremstilling af substituerede oxiraner og triazoler | |
| DK2968099T3 (da) | Metoder og sammensætninger til rengøring og desinfektion af overflader | |
| DK4082530T3 (da) | Proteasehæmmerholdige sammensætninger, sammensætninger omfattende samme og fremgangsmåder til fremstilling og anvendelse af samme | |
| DK3027186T3 (da) | Fremgangsmåde til fremstilling af sammensætninger, der omfatter hyaluronsyre og mepivacainhydrochlorid | |
| DK3079658T3 (da) | Kammer til indkapsling af secernerende celler | |
| DK2739293T3 (da) | Fremgangsmåder og sammensætninger til frembringelse af vaccinavirus | |
| DK3685848T3 (da) | Sammensætninger og fremgangsmåder til behandling af pulmonal hypertension | |
| DK2922955T3 (da) | Sammensætninger og fremgangsmåder til modulering af FGFR3-ekspression | |
| DK3007695T3 (da) | Sammensætninger og fremgangsmåder til behandling af anæmi | |
| DK3363805T3 (da) | Fremgangsmåder til syntese af sphingomyeliner og dihydrosphingomyeliner | |
| DK2994533T3 (da) | Fremgangsmåder og sammensætninger til behandling af cancer | |
| DK2955777T3 (da) | Brændselscelle og fremgangsmåde til fremstilling af samme | |
| DK2552484T3 (da) | Fremgangsmåder og sammensætninger til reducering af parathyroidniveauer |