DK2705144T3 - Polypeptid - Google Patents

Description

DESCRIPTION

Field of the invention [0001] The invention relates to a polypeptide, a corresponding nucleic acid molecule, a construct and/or a vector and/or a cell comprising such nucleic acid molecule and/or a composition comprising said polypeptide, nucleic acid molecule, construct, vector and/or cell. The invention further relates to such polypeptide, corresponding nucleic acid molecule, construct and/or vector and/or cell comprising such nucleic acid molecule and/or composition for medical use, preferably for use in treating an infectious disease. Furthermore, the invention relates to the use of said polypeptide, nucleic acid molecule, construct, vector, cell and/or composition as an antimicrobial, preferably as a food additive or disinfectant, or for detecting bacteria, preferably in a diagnostic application.

Background of the invention [0002] Staphylococcus aureus is a major human pathogen frequently implicated in several serious infectious diseases and food poisoning. Its treatment becomes more and more difficult because of emerging antibiotic resistant strains. Endolysins from phages infecting Staphylococcus aureus have been shown to potentially control these pathogens and can be used for their specific detection. In most cases, major obstacles in the application of endolysins targeting Staphylococcus species are low enzyme activity, difficult production in large quantities and/or protein stability.

[0003] There is always a need for new antimicrobials with improved characteristics on for example antimicrobial activity and/or stability.

Description of the invention [0004] Reported here is the newly characterised Ply2638, the endolysin of S. aureus bacteriophage <t>2638a. The enzyme and several engineered derivatives were expressed in a soluble way in E. Coli and showed surprising stability after lyophilisation as proven by their lytic activity after reconstitution. In addition to a cell wall-binding domain which binds the cell wall of Staphylococcus genera, we showed that two functional domains, i.e. an M23 endopeptidase domain and an amidase domain, are crucial for optimal lytic activity.

[0005] We showed that retrofitting of the enzyme with catalytic domains and/or duplication of the cell wall-binding domain originating from S. aureus Φ11 endolysin, Φ Twort endolysin, and Lysostaphin resulted in a heterologous polypeptide fusion product with an enhanced lytic activity and/or a shifted pH optimum and/or an increased stability after lyophilisation and reconstitution.

[0006] The embodiments of the invention are presented in the claims herein.

Nucleic acid molecule [0007] In a first aspect, there is provided a nucleic acid molecule comprising a first nucleotide sequence, wherein said nucleotide sequence has at least 85% sequence identity with SEQ ID NO: 12, wherein said first nucleotide sequence encodes a cell wall-binding domain binding the peptidoglycan cell wall of Staphylococcus genera, and wherein said nucleic acid molecule further comprises a heterologous nucleotide sequence encoding a lytic domain exhibiting peptidoglycan hydrolase activity [0008] Disclosed is a nucleic acid molecule comprising or consisting of a first nucleotide sequence, said nucleotide sequence having 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 12 (referred is to Table 1 for an overview of all SEQ ID NOs used herein). Preferably, said first nucleotide sequence has a length of at least 282, 285, 290, 300, 310, 320, 330, 340, 350, 360 or 370 nucleotides, more preferably, at least 381 nucleotides and/or a length of at most 510, 480, 450, 420 or 390 nucleotides. Preferably, said nucleic acid molecule has a length of at least 282, 285, 290, 300, 310, 320, 330, 340, 350, 360, 370 nucleotides, more preferably at least 381 nucleotides and/or a length of at most 4500, 4200, 3900 and/or 3600 nucleotides. Preferably, said nucleic acid molecule has a length of at least 1200, 1230, 1260, 1290, 1320, 1350, 1380, 1410, 1440, 1470, 1500, 1530 or 1560 nucleotides and/or a length of at most 4500, 4200, 3900 and/or 3600 nucleotides. Also preferred is a nucleic acid molecule according to the invention with a length of at least 1890, 1920, 1950, 1980, 2010, 2040, 2070, 2100, 2130 or 2160 nucleotides and/or a length of at most 4500, 4200, 3900 and/or 3600 nucleotides. Said first nucleotide sequence encodes a cell wall-binding domain which binds the peptidoglycan cell wall of Staphylococcus genera. Preferably, said first nucleotide sequence originates from S. aureus bacteriophage 02638a endolysin.

[0009] As estimated from alignments with the crystal structure of the C-terminal 92 residues of ALE-1 (Lu et al., J. Biol. Chem., 2006, 281(1):549-58), it was estimated that a minimum of 94 amino acids from the cell wall-binding domain originating from S. aureus bacteriophage 02638a endolysin may be sufficient to direct the enzyme to the cell wall of Staphylococcus genera.

[0010] Binding of a domain to the peptidoglycan cell wall of Staphylococcus genera may be assessed using assays well known to the artisan. In a preferred embodiment, an immunohistochemical technique and/or a gene fusion technique resulting in labelled constructs are used for assessing specific binding of peptides, polypeptides or proteins to the peptidoglycan cell wall of Staphylococcus genera. Quantification methods of signals used in the above mentioned immunohistochemical or fusion techniques are well known in the art.

[0011] In one embodiment, Staphylococcus peptidoglycan cell wall-binding can be quantified using a fluorescent fusion construct comprising a polypeptide comprising a domain encoded by a first nucleotide sequence. Such a cell wall-binding assay is described in detail by Loessner et al (Molecular Microbiology 2002, 44(2): 335-349) and in Example 1. In this assay a solution comprising said fluorescent fusion construct or a negative control, preferably Green Fluorescent Protein (GFP), is subjected to Staphylococcus cells, preferably S. aureus cells, more preferably S. aureus BB255for an indicated time period where after the cells are sedimented by centrifugation together with the bound fluorescent fusion constructs. The fluorescent signal of the Staphylococcus cells exposed to a fluorescent fusion construct subtracted by the fluorescence signal of the Staphylococcus cells exposed to a negative control, preferably GPF, is a measure for cell binding as meant in this disclosure.

[0012] Preferably, within the context of the invention a nucleic acid molecule will be said to encode a polypeptide domain that binds the peptidoglycan cell wall of Staphylococcus genera when using this assay an increase in fluorescent signal of the sedimented cells above the negative control as defined herein is detected. The binding is preferably said to be specific. Preferably, the invention relates to a nucleic acid molecule encoding a polypeptide or a domain which exhibits binding as defined herein of at least 50, 60, 70, 80, 90 or 100, 150 or 200% of peptidoglycan cell wall binding of S. aureus bacteriophage <t>2638a endolysin (Ply2638) encoded by SEQ ID NO: 1.

[0013] In an embodiment, the invention relates to a nucleic acid molecule that has at least 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91,92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 1 encoding for S. aureus bacteriophage Φ2638 endolysin.

[0014] The nucleic acid molecule comprises in addition to said first nucleotide sequence, a heterologous nucleotide sequence encoding a lytic domain that exhibits peptidoglycan hydrolase activity as defined later herein. Said nucleic acid molecule comprising heterologous nucleotide sequences being defined herein as a "retrofitted construct".

[0015] As used herein the term "heterologous sequence" or "heterologous nucleic acid" is one that is not naturally found operably linked as neighbouring sequence of said first nucleotide sequence. As used herein, the term "heterologous" may mean "recombinant". "Recombinant" refers to a genetic entity distinct from that generally found in nature. As applied to a nucleotide sequence or nucleic acid molecule, this means that said nucleotide sequence or nucleic acid molecule is the product of various combinations of cloning, restriction and/or ligation steps, and other procedures that result in the production of a construct that is distinct from a sequence or molecule found in nature.

[0016] A "peptidoglycan hydrolase activity" herein also defined as a "lytic activity" can be assessed by methods well known to the artisan. In an embodiment, lytic activity can be assessed spectrophotometrically by measuring the drop in turbidity of substrate cell suspensions. Preferably, lytic activity can be assessed spectrophotometrically measuring the drop in turbidity of a S. aureus suspension, wherein turbidity is quantified by measuring OD595 spectrophotometrically (Libra S22, Biochrom). More preferably, 200 nM of a polypeptide encoded by a nucleic acid molecule as identified herein is incubated together with an S. aureus suspension having an initial Οϋβοο of 1 ± 0.05, as assessed spectrophotometrically (Libra S22, Biochrom), in PBS buffer pH 7.4, 120 mM sodium chloride for 30 min at 37°C. The drop in turbidity is calculated by subtracting the OD595 after 30 min of incubation from the OD595 before 30 min of incubation. Within the context of the invention a nucleic acid molecule will be said to comprise a nucleic acid sequence encoding a lytic domain when using this assay a drop in turbidity of at least 10, 20, 30, 40, 50 or 60% is detected. Preferably, a drop of at least 70% is detected. Preferably, the invention relates to a nucleic acid molecule encoding a polypeptide which exhibits a lytic activity of at least 50, 60, 70, 80, 90, 100, 150 or 200% or more of a lytic activity of S. aureus bacteriophage <t>2638a endolysin (Ply2638) encoded by SEQ ID NO: 1.

[0017] A nucleic acid molecule of the invention does not consist of SEQ ID NO:1. SEQ ID NO: 1 encodes for S. aureus bacteriophage Φ2638 endolysin.

[0018] The nucleic acid molecule comprising said first nucleotide sequence as identified herein further comprises as a lytic domain a second and third nucleotide sequences, wherein said second sequence encodes an endopeptidase domain and third nucleotide sequence encodes an amidase domain. Disclosed is a nucleic acid molecule comprising said first nucleotide sequence, wherein said nucleic acid molecule has 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 1, or wherein said nucleic acid molecule further comprises a heterologous nucleotide sequence encoding a lytic domain.

[0019] An endopeptidase domain as used herein preferably cleaves the pentaglycine cross-bridges (Trayer, H. R. and Buckley, C. E. (1970) Molecular properties of lysostaphin, a bacteriolytic agent specific for Staphylococcus aureus. J. Biol. Chem. 245, 4842-4846) that are found in the cell wall of Staphylococcus genera, preferably in the cell wall of S. aureus, S. simulans and S. carnosus. An amidase domain as used herein preferably hydrolyzes gamma-glutamyl-containing substrates. The functionality and activity of these domains in a polypeptide can be confirmed by characterizing the cleavage products upon incubation of said polypeptides containing any of these domains with purified peptidoglycan. Preferably, each of the nucleotide sequences encoding the second or third domain is of bacterial or bacteriophage origin. In a preferred embodiment, said second and third nucleotide sequences originate from a gene encoding for an enzyme selected from the group consisting of S. aureus bacteriophage <t>2638a endolysin, S. aureus bacteriophage Φ11 endolysin, S. aureus bacteriophage ΦΤνοΓί endolysin and S. Simulans lysostaphin. Preferably, said second nucleotide sequence has 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 14 or 15 and said third nucleotide sequence has 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 16 or 17.

[0020] The invention encompasses all constructs as defined herein containing the functional domains as meant in the invention at any possible location within the construct. In a preferred embodiment, a nucleic acid molecule as defined herein encodes for a polypeptide with a C-terminal domain encoded by a first nucleic acid sequence as identified herein, vtfiich is shown herein to encode for functional polypeptides able to target for Staphylococcus genera. Even more preferred is a nucleic acid molecule as defined herein comprising a nucleic acid molecule that has 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91,92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 9. SEQ ID NO: 9 comprises a first nucleotide sequence encoding a C-terminal SH3b homologue cell wall-binding domain (both domains from Ply2638 encoded by SEQ ID NO: 1: Leu138 - Lys486), a second nucleotide sequence encoding a polypeptide comprising an N-terminal M23 glycyl-glycine endopeptidase homologue domain (mature Lysostaphin encoded by SEQ ID NO: 33: Alai - Gly154) and a third nucleotide sequence encoding a central amidase-2 homologue domain. It has a theoretical size of 58.266 kDa. A polypeptide encoded by SEQ ID NO: 9 differs from S. aureus bacteriophage ¢26388 endolysin in that the N-terminal M23 endopeptidase domain is substituted by an M23 endopeptidase domain from S. Simulans lysostaphin. We showed here that a polypeptide encoded by SEQ ID NO: 9 demonstrated at least 20% increased lytic activity as compared to S. aureus bacteriophage Φ26388 endolysin while the lytic activity is maintained after lyophilisation and reconstitution. In a preferred embodiment, a nucleic acid molecule comprising said first, second and third nucleotide sequences encodes for a polypeptide exhibiting a lytic activity of at least 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 or 2 fold as compared to a lytic activity of S. aureus bacteriophage ¢26388 endolysin encoded by SEQ ID NO:1. In a preferred embodiment, a nucleic acid molecule comprising said first, second and third nucleotide sequences encodes for a polypeptide exhibiting a decrease in lytic activity of at most 1,2, 3,4, 5, 6, 7, 8, 9 or 10% after lyophilisation and reconstitution as defined herein as compared to freshly prepared polypeptide, wherein "freshly prepared" is preferably defined herein as at most 2 days storage at 1.63 mg/mL in lyophilisation buffer (50mM Tris, 500mM sucrose, 200mM mannitol, 0.05% polysorbate 20 + 50% glycerol) at -20 °C and thawed immediately before assessing lytic activity in an assay as identified herein.

[0021] Lyophilisation and reconstitution is defined herein as dehydration by freeze-drying and subsequent reconstitution of the sample by adding water. In an embodiment, lyophilisation and reconstitution may be done by dialyzing against 3 changes of 300 ml lyophilization buffer (50 mM phosphate or Tris, 500 mM sucrose, 200 mM mannitol, pH 7.4) aliquot and freezing in the gaseous phase of liquid nitrogen. The freeze-drying can be done under standard conditions, preferably at -40°C and vacuum at 75 mTorr for 60 minutes, followed by increasing temperature during 5 hours to -10°C and another 60 minutes at -10°C at the same vacuum levels. As final step, temperature is preferably increased to 25°C during 10 hours. Samples are reconstituted by the addition of water.

[0022] In another preferred embodiment, a nucleic acid molecule as defined herein comprises in addition to the above identified first, second and third nucleotide sequences at least one duplicate identical or heterologous first, second and/or third nucleotide sequence. Preferably, a nucleic acid molecule as defined herein comprises in addition to said first, second and third nucleotide sequences, a duplicate identical first nucleotide sequence. We showed here that duplication of the first nucleotide sequence as defined herein encoding a cell wall-binding domain results in a polypeptide preferably as encoded by SEQ ID NO: 20 which exposes at least 5, 10, 20, 30, 20 or 40% increased lytic activity as compared to a lytic activity of a reference polypeptide encoded by a nucleic acid molecule lacking such duplicate first domain or as compared to a lytic activity of S. aureus bacteriophage <t>2638a endolysin encoded by SEQ ID NO:1 as assessed in an assay as identified herein, more specifically using equimolar amount of a polypeptide and a modified PBS buffer containing 200, 300, 400, or 1000nM NaCI. This embodiment also encompasses a heterologous nucleic acid molecule in which said first, second and third nucleotide sequences originate from the same source being S. aureus bacteriophage<t>2638a, said nucleic acid molecule comprising a sequence that has 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO:1 and an additional duplicate identical or heterologous first, second and/or third nucleotide sequence. Also preferred is a nucleic acid molecule as defined herein comprising a nucleotide sequence that has 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity wth SEQ ID NO: 6 and 20.

[0023] In another preferred embodiment, a nucleic acid molecule as defined herein comprises a fourth nucleotide sequence encoding a CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain. More preferably, said fourth nucleotide sequences originates from S. aureus bacteriophage Φ11 or S. aureus bacteriophage OTwort endolysin. Even more preferably, said fourth nucleotide sequence has 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 18 or SEQ ID NO: 19. Preferably, a nucleic acid molecule as defined herein comprises a nucleotide sequence that has 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 5 or 7. We showed here that a molecule comprising said first, second, third and fourth nucleotide sequences as defined by SEQ ID NO: 5 encodes for a polypeptide exhibiting an increased lytic activity and/or a shifted, preferably decreased pH optimum as compared to a polypeptide encoded by a construct lacking said fourth domain and/or compared to S. aureus bacteriophage <P2638a endolysin encoded by SEQ ID NO: 1. Lytic activity was assessed spectrophotometrically and under the conditions as earlier defined herein. Preferably, said polypeptide exhibits an increase in a lytic activity of at least 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, or 2 fold as compared to a lytic activity of a polypeptide encoded by a reference polypeptide differing from said polypeptide only in lacking said fourth domain or as compared to a lytic activity of S. aureus bacteriophage 02638a endolysin encoded by SEQ ID NO: 1. Even more preferably, said polypeptide exhibits an increase in a lytic activity of at least 2.5 fold as compared to the defined reference polypeptide or a polypeptide encoded by SEQ ID NO: 1.

[0024] A shifted or decreased pH optimum is defined herein as a shift or decrease in optimal lytic activity to a lower pH value, where ionic strength is kept constant. Lytic activity is preferably assessed spectrophotometrically as defined herein. Preferably, the pH optimum of a lytic activity is decreased 0.5 - 1 pH unit as compared to a lytic activity of a polypeptide encoded by a reference polypeptide differing from said polypeptide only in lacking said fourth domain or as compared to a lytic activity of S. aureus bacteriophage 02638a endolysin encoded by SEQ ID NO:1.

[0025] The invention preferably relates to a nucleic acid molecule comprising a first, second, third and optionally fourth nucleotide sequences as identified herein encoding a polypeptide which has the same lytic activity and/or the same pH optimum or which has an increased lytic activity and/or a decreased pH optimum as compared to a lytic activity ofS. aureus bacteriophage <t>2638a endolysin encoded by SEQ ID NO: 1. The same being identified herein as no detectable difference when using the assay as identified herein or a method well known by the artisan. The current invention also relates to a nucleic acid molecule comprising a first, second, and fourth nucleotide sequence as identified herein encoding a polypeptide which has the same lytic activity and/or the same pH optimum or which has an increased lytic activity and/or a decreased pH optimum as compared to a lytic activity of S. aureus bacteriophage 02638a endolysin encoded by SEQ ID NO: 1. The current invention also relates to a nucleic acid molecule comprising a first, third, and fourth nucleotide sequence as identified herein encoding a polypeptide which has the same lytic activity and/or the same pH optimum or which has an increased lytic activity and/or a decreased pH optimum as compared to a lytic activity of S. aureus bacteriophage <t>2638a endolysin encoded by SEQ ID NO: 1. Each of the nucleotide sequences identified herein, i.e. first, second, third, fourth nucleotide sequences, encoding the individual domain of a polypeptide defined herein can be assembled by any usual method known for constructing and assembling nucleic acid fragments which are well known to those skilled in the art and widely described in the literature (Sambrook, Maniatis et al. (1989) and illustrated experimental part of the disclosure. In a preferred embodiment, a first, second, third and/or fourth nucleotide sequences are operably linked together.

[0026] Accordingly, a nucleic acid molecule of the invention encodes a polypeptide, preferably a polypeptide as identified herein which is able to bind Staphylococcus genera via the cell wall-binding domain encoded by a first nucleotide sequence as defined herein and/or lyse said bacteria via an endopeptidase and/or amidase domain and optionally a CHAP domain encoded by a second, third and fourth nucleotide sequence, respectively, as defined herein.

[0027] In a preferred embodiment, a nucleic acid molecule of the invention as defined herein optionally comprises a sequence encoding a tag for ease of purification. Preferably, said tag is selected from, but is not limited to, the group consisting of a FLAG-tag, poly(His)-tag, HA-tag and Myc-tag. More preferably said tag is a 6xHis-tag. Even more preferably, said tag is an N-terminal 6xHis-tag identical to SEQ ID NO: 43.

Polypeptide [0028] In a further aspect, there is provided a polypeptide encoded by a nucleic acid molecule according to the invention. This polypeptide comprises a cell wall-binding domain and an endopeptidase domain and/or an amidase domain as defined in the previous section.

[0029] A polypeptide domain encompassed by the current invention preferably has 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 35, 36, 37, 38, 39, 40, 41 and/or 42. Preferably, a polypeptide domain encompassed by the current invention preferably comprises one or more putative linkers and has 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 51, 52, 53, 54, 55, 56, 57 and/or 58.

[0030] A polypeptide encompassed by the current invention preferably has 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91,92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 21, 25, 26, 27, 29 and/or 32. More preferably, a polypeptide encompassed by the current invention preferably has 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 25, 26, 27, 29 and/or 32 with SEQ ID NO: 25, 26, 27, 29 and/or 32.

[0031] In an embodiment of the current invention, a polypeptide preferably has at least 80% sequence identity with SEQ ID NO: 21, 25, 26, 27, 29, 32, 35, 36, 37, 38, 39, 40, 41, and/or 42 encoded by a nucleic acid construct with at least 80% identity with SEQ ID NO: 1, 5, 6, 7, 9, 20, 12, 13, 14, 15, 16, 17, 18 and/or 19, respectively. More preferably, a polypeptide of the current invention has at least 80% sequence identity with SEQ ID NO: 25, 26, 27, 29, 32, 35, 36, 37, 38, 39, 40, 41, and/or 42 encoded by a nucleic acid construct with at least 80% identity with SEQ ID NO: 5, 6, 7, 9, 20, 12, 13, 14, 15, 16, 17, 18 and/or 19, respectively.

[0032] A polypeptide according to the invention may have a length of at least 94, 95, 96, 100, 110 or 120 amino acids, preferably 127 amino acids and/or at most 1500, 1400, 1300 or 1200 amino acids. Preferably, said polypeptide has a length of at least 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510 or 520 amino acids and/or at most 1500, 1400, 1300 or 1200 amino acids. Also preferred is a polypeptide according to the invention with a length of at least 630, 640, 650, 660, 670, 680, 690, 700, 710 or 720 amino acids and/or at most 1500, 1400, 1300 or 1200 amino acids.

[0033] An amino acid or nucleotide sequence, encompassed by the present invention, may be derived from one of the sequences as identified herein by substituting, inserting, deleting, or adding one, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18,20 or more nucleotides or amino acids, respectively. An amino acid sequence, encompassed by the present invention, may be derived from one of the sequences as identified herein by adding an additional N- or C- terminal amino acids or chemical moieties to increase stability, solubility and activity.

[0034] An embodiment of the invention encompasses a variant polypeptide. A variant polypeptide may be a non-naturally occurring form of the polypeptide. A polypeptide variant may differ in some engineered way from the polypeptide isolated from its native source. A variant may be made by site-directed mutagenesis starting from the nucleotide sequence of SEQ ID NO: 1,5, 6, 7, 9, 12, 13, 14, 15, 16, 17, 18, 19 and/or 20. Preferably, a polypeptide variant contains mutations that do not alter the biological function of the encoded polypeptide. According to a preferred embodiment, a polypeptide variant exhibits Staphylococcus peptidoglycan cell wall-binding and/or a lytic activity which is the same or enhanced as compared to the Staphylococcus peptidoglycan cell-wall binding and/or lytic activity of S. aureus bacteriophage 02638a endolysin encoded by SEQ ID NO:1 . A polypeptide variant of the invention preferably is a variant of SEQ ID NO: 21, 25, 26, 27, 29, 32, 35, 36, 37, 38, 39, 40, 41 and/or 42. A polypeptide variant with the same or an enhanced Staphylococcus peptidoglycan cell-wall binding and/or lytic activity is a polypeptide exhibiting a Staphylococcus aureus peptidoglycan cell-wall binding and/or lytic activity, which is the same or increased compared to the Staphylococcus peptidoglycan cell-wall binding and/or lytic activity ofS. aureus bacteriophage 02638a endolysin encoded by SEQ ID NO:1 measured in an assay as earlier identified herein.

[0035] According to another preferred embodiment, a nucleotide sequence of the invention is a variant of the nucleotide sequences of SEQ ID NO: 1, 5, 6, 7, 9, 12, 13, 14, 15, 16, 17, 18, 19 and/or 20. Nucleotide sequence variants may be used for preparing a polypeptide variant as defined earlier. A nucleic acid variant may be a fragment of any of the nucleotide sequences as defined above. A nucleic acid variant may also be a nucleotide sequence that differs from SEQ ID NO: 1,5, 6, 7, 9, 12, 13, 14, 15, 16, 17, 18, 19 and/or 20 by virtue of the degeneracy of the genetic code. A nucleic acid variant may also be an allelic variant of SEQ ID NO: 1, 5, 6, 7, 9, 12,13, 14, 15, 16, 17, 18, 19 and/or 20. An allelic variant denotes any of two or more alternative forms of a gene occupying the same chromosome locus. A preferred nucleic acid variant is a nucleotide sequence, which contains silent mutation(s). Alternatively or in combination, a nucleic acid variant may also be obtained by introduction of nucleotide substitutions, which do not give rise to another amino acid sequence of the polypeptide encoded by the nucleotide sequence, but which corresponds to the codon usage of the host organism intended for production of the polypeptide of the invention. According to a preferred embodiment, a nucleic acid variant encodes a polypeptide still exhibiting its biological function. More preferably, a nucleotide sequence variant encodes a polypeptide exhibiting Staphylococcus peptidoglycan cell wall-binding and/or a lytic activity. Even more preferably, a nucleic acid variant encodes a polypeptide with enhanced Staphylococcus peptidoglycan cell wall-binding and/or lytic activity as defined earlier. Nucleic acids encoding a polypeptide exhibiting Staphylococcus peptidoglycan cell wall-binding and/or lytic activity may be isolated from any microorganism.

[0036] All these variants can be obtained using techniques known to the skilled person, such as screening of library by hybridisation (southern blotting procedures) under low to medium to high hybridisation conditions with for the nucleotide sequence SEQ ID NO: 1, 5, 6, 7, 9, 12, 13, 14, 15, 16, 17, 18, 19 and/or 20 or a variant thereof which can be used to design a probe. Low to medium to high stringency conditions means prehybridization and hybridization at 42°C in 5X SSPE, 0.3% SDS, 200pg/ml sheared and denatured salmon sperm DNA, and either 25% 35% or 50% formamide for low to medium to high stringencies respectively. Subsequently, the hybridization reaction is washed three times for 30 minutes each using 2XSSC, 0.2%SDS and either 55°C, 65 °C, or 75°C for low to medium to high stringencies.

[0037] The sequence information as provided herein should not be so narrowly construed as to require inclusion of erroneously identified bases. The skilled person is capable of identifying such erroneously identified bases and knows how to correct for such errors.

Nucleic acid construct [0038] In a further aspect, there is provided a nucleic acid construct comprising a nucleic acid molecule according to the invention. This nucleic acid construct comprises a first nucleic acid sequence encoding a polypeptide comprising a cell wallbinding domain and further comprises a second or third and optionally fourth nucleic acid sequence as defined in the previous section.

[0039] The invention also relates to an expression vector comprising a nucleic acid construct of the invention. Preferably, an expression vector comprises a nucleotide sequence of the invention, which is operably linked to one or more control sequences, which direct the production or expression of the encoded polypeptide in a cell, a subject, or a cell-free expression system.

[0040] An expression vector may be seen as a recombinant expression vector. This vector can be constituted by a plasmid, a cosmid, a bacteriophage or a virus which is transformed by introducing a nucleic acid molecule according to the invention. Such transformation vectors according to the host organism to be transformed are well known to those skilled in the art and widely described in the literature.

[0041] Afurther subject of the invention is a process for the transformation of host organisms, by integrating a least one nucleic acid molecule of the invention, which transformation may be carried out by any suitable known means which have been widely described in the specialist literature and in particular in the references cited in the present application, more particularly by the vector according to the invention.

Cell [0042] In a further aspect, the present invention relates to a cell, which comprises a nucleic acid construct or an expression vector of the invention as defined herein. A cell may be any microbial, prokaryotic or eukaryotic cell, which is suitable for expression of the polypeptide of the invention. In a preferred embodiment, said cell is an E. Coli. In an even more preferred embodiment, said cell is E. coli CLIblue MRF.

Method [0043] In a further aspect, there is provided a method for producing, optionally purifying and optionally freeze-drying a polypeptide according to the invention. Said method comprising the steps of: 1. i) producing said polypeptide in a cell according to the invention comprising a nucleic acid construct as defined in the previous section, optionally 2. ii) purifying said polypeptide, and optionally 3. iii) freeze-drying said purified polypeptide.

[0044] In a preferred embodiment, an E. Coll is used in step i) for producing a polypeptide using recombinant technologies. More preferably an E. coli XLIblueMRF is used in step i) for producing a polypeptide using recombinant technologies Preferably, in step ii), IMAC and Econo-Pac Chromatography columns (Biorad) packed with 5mL low density Nickel chelating agarose beads (ABT beads) in combination with gravity flow is used to purify said (6xHis-tagged recombinant) polypeptides. The eluted polypeptide can be dialyzed for 2, 4, and 12 hours against 3x11 lyophilization buffer, said buffer preferably comprising 50 mM phosphate, 500mM sucrose, 200mM mannitol, 0.005% polysorbate20, pH 7.4.

Method [0045] In a further aspect, the invention also relates to a method for producing a polypeptide with an enhanced lytic activity by treating a polypeptide according to the invention or as obtainable by the method described above. Said treatment comprises substituting a divalent metal ion for increasing a lytic activity as compared to an untreated polypeptide, preferably said method comprising the steps of: 1. i) dialyzing said polypeptide against a buffer comprising a chelating compound; 2. ii) dialyzing said polypeptide against a divalent metal ion-containing buffer, preferably said divalent metal ion being selected from the group consisting of Mn2+, Co2+, Cu2+, and Zn2+.

[0046] A "chelating compound" being defined herein as a compound that binds a metal ion. Well known chelating compounds are ethylene diammine tetraacetic acid (EDTA) and ethylene glycol tetraacetic acid (EGTA). Preferably EDTA is used in step i) of the method of the invention.

[0047] Preferably, the divalent metal ion of step ii) is selected from the group consisting Mn2+, Co2+, Cu2+, more preferably, said divalent metal ion is selected from the group consisting of Mn2+ and Co2+, even more preferably said divalent metal ion is Mn2+.

[0048] We showed that substituting a divalent metal ion by any of the above defined resulted in an increase of a lytic activity of Ply2638 of 2 - 2.5 fold. Lytic activity was assessed spectrophotometrically as defined herein. Preferably, said method leads to an increase in a lytic activity of at least 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 or 2 fold as compared to an untreated polypeptide. Even more preferably, the method leads to an increase in a lytic activity of at least 2.5 fold. Preferably, the treated polypeptide exhibits a 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 to 2 fold increase in lytic activity as compared to the untreated polypeptide encoded by SEQ ID NO: 1.

Composition [0049] In a further aspect, there is provided a composition comprising a nucleic acid molecule or a nucleic acid construct or a polypeptide or a vector or a cell according to the invention. Preferably, the composition exhibits a lytic activity as defined herein. More preferably, said composition is for use as a medicament. This medicament is preferably for treating, preventing and/or delaying an infectious disease. The invention also relates to a pharmaceutical or medical composition. Even more preferably, the invention relates to a pharmaceutical or medical composition for the treatment of an infectious disease. Preferably, the invention relates to a pharmaceutical or medical composition for the treatment of an infectious disease caused by a bacterium, preferably a bacterium of the genus Staphylococcus, more preferably a bacterium of the species S. aureus. Preferably, said infectious disease is a skin infection, mastitis, pneumonia, meningitis, endocarditis, Toxic Shock Syndrome (TSS), sepsis, septicemia, bacteremia, or osteomyelitis. Preferably, said skin infection is selected from the group of pimples, impetigo, boils, furuncles, cellulitis folliculitis, carbuncles, scaled skin syndrome and abscesses.

[0050] A composition according to the invention may comprise a mixture of different nucleic acid molecules, and/or nucleic acid constructs and/or polypeptides and/or vectors and/or cells according to the invention.

[0051] A composition according to the invention may comprise one or more additional active ingredients. Active preferably being defined herein as showing a lytic activity as defined herein. Preferably, said one or more additional active ingredients are selected from the group consisting of a bacteriophage or phage and antibiotic. A phage encompassed herein can be any phage known in literature. Preferably, a phage encompassed by the present invention belongs, but is not limited, to a family of the list consisting of Myoviridae, Slphovirldae and Podovirldae. A phage encompassed by the present invention may also belong to a family of the list consisting of Tectlvirldae, Cortlcovirldae, Llpothrlxvirldae, Plasmavirldae, Rudlvirldae, Fusellovirldae, Inovlrldae, Mlcrovirldae, Leviviridae and Cystoviridae. More preferably, said one or more active ingredients comprise and/or consist of lysostaphin, preferably S. Slmulans lysostaphin having 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% sequence identity with SEQ ID NO: 34, more preferably S. Slmulans lysostaphin of SEQ ID NO: 34.). Even more preferably, said one or more active ingredients comprise and/or consist of both one or more different bacteriophages and lysostaphin, preferably, one or more different phages and S. Slmulans lysostaphin (SEQ ID NO: 34). Within the context of this invention, a combination of active ingredients as defined herein can be administered sequentially and/or simultaneously.

[0052] A composition as defined herein may further comprise a pharmaceutically acceptable carrier. Such composition is preferably for use as a medicine or as a medicament. Preferably the medicament is used in the treatment of infectious diseases. A composition may be in the liquid, solid or semi-liquid or semi-solid form.

[0053] Acomposition of the invention can be used to treat animals, including humans, infected with S. aureus. Any suitable route of administration can be used to administer said composition including but not limited to: oral, aerosol or other device for delivery to the lungs, nasal spray, intravenous, intramuscular, intraperitoneal, intrathecal, vaginal, rectal, topical, lumbar puncture, intrathecal, and direct application to the brain and/or meninges.

[0054] A composition comprising a nucleic acid molecule or a nucleic acid construct or a polypeptide or a vector or a cell according to the invention is preferably said to be active, functional or therapeutically active or able to treat, prevent and/or delay an infectious disease when it decreases the amount of a Staphylococcus genera present in a patient or in a cell of said patient or in a cell line or in a cell free in vitro system and preferably means that 99%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 5% or less of the initial amount of a Staphylococcus genera, is still detectable. Preferably no Staphylococcus genera is detectable. In this paragraph, the expression "amount of Staphylococcus genera" preferably means alive Staphylococcus genera. Staphylococcus genera may be detected using standard techniques known by the artisan such as immunohistochemical techniques using Staphylococcus specific antibodies, tube coagulase tests that detect staphylocoagulase or "free coagulase", detection of surface proteins such as clumping factor (slide coagulase test) and/or protein A (commercial latex tests). Alive Staphylococcus genera may be detected using standard techniques known by the artisan such as microbiological bacterial culture techniques and/or real-time quantitative reverse transcription polymerase chain reaction to assay for bacterial mRNA.

Said decrease is preferably assessed in a tissue or in a cell of an individual or a patient by comparison to the amount present in said individual or patient before treatment with said composition or polypeptide of the invention. Alternatively, the comparison can be made with a tissue or cell of said individual or patient which has not yet been treated with said composition or polypeptide in case the treatment is local.

[0055] A composition comprising a nucleic acid molecule or a nucleic acid construct or a polypeptide or a vector or a cell according to the invention may be administered to a patient or of a cell, tissue or organ or said patient at least one week, one month, six month, one year or more.

[0056] In another embodiment, the invention relates to a non-medical composition exhibiting a binding and/or lytic activity according to the invention. Preferably, such composition relates to an antimicrobial. Preferably, such composition relates to an antimicrobial for lysing a bacterium, preferably a bacterium of the genus Staphylococcus, more preferably a bacterium of the species S. aureus. Preferably, such composition relates to an antimicrobial as food preservative or disinfectant.

Use [0057] In a further aspect, the invention relates to the use of a polypeptide according to the invention, a nucleic acid molecule according to the invention, a construct comprising such nucleic acid molecule, a vector comprising such construct, a cell comprising such vector and/or a composition comprising any of the above, preferably as antimicrobial. Preferably, use is as an antimicrobial for lysing a bacterium, preferably a bacterium of the genus Staphylococcus, more preferably a bacterium of the species S. aureus. Preferably the use relates to an antimicrobial as food preservative or disinfectant. Possibly, such food preservatives or disinfectants are used together with other antimicrobial agents. Preferably, such food preservatives or disinfectants are used in combination with one or more additional active ingredients as defined herein. Preferably, said one or more additional active ingredients are selected from the group consisting of a bacteriophage or phage and antibiotic as defined herein.

[0058] The polypeptide, nucleic acid molecule, construct, vector, cell and/or composition according to the invention can be applied on or into food products, and/or into various physical sites to be disinfected, by a number of means including, but not limited to, admixing said polypeptide and/or cell containing polypeptide of the invention into the food products, spraying said polypeptide and/or cell containing the polypeptide of the invention onto the foodstuffs or physical sites to be disinfected.

[0059] A polypeptide of the invention can be isolated from a cell or a cell containing said polypeptide of the invention can be directly applied or administered without isolation of said polypeptide. For example, a cell which produces a polypeptide of the invention could be administered to a subject (human or animal) or applied to a surface where the polypeptide of the invention would be secreted into food, onto a surface or into the subject's gut. The polypeptide of the invention can then bind and lyse a bacterium of the genus Staphylococcus, more preferably a bacterium of the species S. aureus, present in this environment.

[0060] Further encompassed is the use of a polypeptide according to the invention, a nucleic acid molecule encoding such polypeptide, a construct comprising such nucleic acid molecule, a vector comprising such construct, a cell comprising such vector and/or a composition comprising any of the above, for detecting bacteria of the genus Staphylococcus, more preferably a bacterium of the species S. aureus. Preferably, said polypeptide, nucleic acid molecule, construct, vector, cell and/or composition is used in a diagnostic application. Possibly said polypeptide, nucleic acid molecule, a construct, a vector, cell and/or a composition is used together with other detection agents.

Definitions Sequence identity [0061] "Sequence identity" is herein defined as a relationship between two or more amino acid (peptide, polypeptide, or protein) sequences or two or more nucleic acid (nucleotide, polynucleotide) sequences, as determined by comparing the sequences. In the art, "identity" also means the degree of sequence relatedness between amino acid or nucleotide sequences, as the case may be, as determined by the match between strings of such sequences. "Similarity" between two amino acid sequences is determined by comparing the amino acid sequence and its conserved amino acid substitutes of one peptide or polypeptide to the sequence of a second peptide or polypeptide. In a preferred embodiment, identity or similarity is calculated over the whole SEQ ID NO as identified herein. "Identity" and "similarity" can be readily calculated by known methods, including but not limited to those described in Computational Molecular Biology, Lesk, A M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993 ; Computer Analysis of Sequence Data, Part I, Griffin, A. M., and Griffin, H. G., eds., Flumana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heine, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991; and Carillo, H„ and Lipman, D„ SIAM J. Applied Math., 48:1073 (1988).

[0062] Preferred methods to determine identity are designed to give the largest match between the sequences tested. Methods to determine identity and similarity are codified in publicly available computer programs. Preferred computer program methods to determine identity and similarity between two sequences include e.g. the GCG program package (Devereux, J., et al., Nucleic Acids Research 12 (1): 387 (1984)), BestFit, BLASTP, BLASTN, and FASTA ( Altschul, S. F. et al., J. Mol. Biol. 215:403-410 (1990). The BLAST X program is publicly available from NCBI and other sources (BLAST Manual, Altschul, S., et al., NCBI NLM NIH Bethesda, MD 20894; Altschul, S., et al., J. Mol. Biol. 215:403-410 (1990). The well-known Smith Waterman algorithm may also be used to determine identity.

[0063] Preferred parameters for polypeptide sequence comparison include the following: Algorithm: Needleman and Wunsch, J.

Mol. Biol. 48:443-453 (1970); Comparison matrix: BLOSSUM62 from Hentikoff and Hentikoff, Proc. Natl. Acad. Sci. USA. 89:10915-10919 (1992); Gap Penalty: 12; and Gap Length Penalty: 4. A program useful with these parameters is publicly available as the "Ogap" program from Genetics Computer Group, located in Madison, Wl. The aforementioned parameters are the default parameters for amino acid comparisons (along with no penalty for end gaps).

[0064] Preferred parameters for nucleic acid comparison include the following: Algorithm: Needleman and Wunsch, J. Mol. Biol. 48:443-453 (1970); Comparison matrix: matches=+10, mismatch=0; Gap Penalty: 50; Gap Length Penalty: 3. Available as the Gap program from Genetics Computer Group, located in Madison, Wis. Given above are the default parameters for nucleic acid comparisons.

[0065] Optionally, in determining the degree of amino acid similarity, the skilled person may also take into account so-called "conservative" amino acid substitutions, as will be clear to the skilled person. Conservative amino acid substitutions refer to the interchangeability of residues having similar side chains. For example, a group of amino acids having aliphatic side chains is glycine, alanine, valine, leucine, and isoleucine; a group of amino acids having aliphatic-hydroxyl side chains is serine and threonine; a group of amino acids having amide-containing side chains is asparagine and glutamine; a group of amino acids having aromatic side chains is phenylalanine, tyrosine, and tryptophan; a group of amino acids having basic side chains is lysine, arginine, and histidine; and a group of amino acids having sulphur-containing side chains is cysteine and methionine. Preferred conservative amino acids substitution groups are: valine-leucine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, and asparagine-glutamine. Substitutional variants of the amino acid sequence disclosed herein are those in which at least one residue in the disclosed sequences has been removed and a different residue inserted in its place. Preferably, the amino acid change is conservative. Preferred conservative substitutions for each of the naturally occurring amino acids are as follows: Ala to ser; Arg to lys; Asn to gin or his; Asp to glu; Cys to ser or ala; Gin to asn; Glu to asp; Gly to pro; His to asn or gin; lie to leu or val; Leu to ile or val; Lys to arg; gin or glu; Met to leu or ile; Phe to met, leu or tyr; Ser to thr; Thr to ser; Trp to tyr; Tyr to trp or phe; and, Val to ile or leu.

Nucleic acid construct, transformation, expression vector, operablv linked, expression, control sequences. polypeptide

Construct [0066] A nucleic acid molecule is represented by a nucleotide sequence. A polypeptide is represented by an amino acid sequence. A nucleic acid construct is defined as a nucleic acid molecule which is isolated from a naturally occurring gene or which has been modified to contain segments of nucleic acids which are combined or juxtaposed in a manner which would not otherwise exist in nature. A nucleic acid molecule is represented by a nucleotide sequence. Optionally, a nucleotide sequence present in a nucleic acid construct is operably linked to one or more control sequences, which direct the production or expression of said peptide or polypeptide in a cell or in a subject.

[0067] "Operably linked" is defined herein as a configuration in which a control sequence is appropriately placed at a position relative to the nucleotide sequence coding for the polypeptide of the invention such that the control sequence directs the production/expression of the peptide or polypeptide of the invention in a cell and/or in a subject.

[0068] "Operably linked" may also be used for defining a configuration in which a sequence is appropriately placed at a position relative to another sequence coding for a functional domain such that a chimeric polypeptide is encoded in a cell and/or in a subject.

Expression will be understood to include any step involved in the production of the peptide or polypeptide including, but not limited to, transcription, post-transcriptional modification, translation, post-translational modification and secretion.

[0069] Control sequence is defined herein to include all components which are necessary or advantageous for the expression of a polypeptide. At a minimum, the control sequences include a promoter and transcriptional and translational stop signals. Optionally, a promoter represented by a nucleotide sequence present in a nucleic acid construct is operably linked to another nucleotide sequence encoding a peptide or polypeptide as identified herein.

[0070] The term "transformation" refers to a permanent or transient genetic change induced in a cell following the incorporation of new DNA(i.e. DNA exogenous to the cell). When the cell is a bacterial cell, as is intended in the current invention, the term usually refers to an extrachromosomal, self-replicating vector which harbors a selectable antibiotic resistance.

[0071] An expression vector may be any vector which can be conveniently subjected to recombinant DNA procedures and can bring about the expression of a nucleotide sequence encoding a polypeptide of the invention in a cell and/or in a subject. As used herein, the term “promoter" refers to a nucleic acid fragment that functions to control the transcription of one or more genes or nucleic acids, located upstream with respect to the direction of transcription of the transcription initiation site of the gene. It is related to the binding site identified by the presence of a binding site for DNA-dependent RNA polymerase, transcription initiation sites, and any other DNA sequences, including, but not limited to, transcription factor binding sites, repressor and activator protein binding sites, and any other sequences of nucleotides known to one skilled in the art to act directly or indirectly to regulate the amount of transcription from the promoter. Within the context of the invention, a promoter preferably ends at nucleotide -1 of the transcription start site (TSS).

[0072] "Polypeptide" as used herein refers to any peptide, oligopeptide, polypeptide, gene product, expression product, or protein. A polypeptide is comprised of consecutive amino acids. The term "polypeptide" encompasses naturally occurring or synthetic molecules.

[0073] In this document and in its claims, the verb "to comprise" and its conjugations is used in its non-limiting sense to mean that items following the word are included, but items not specifically mentioned are not excluded. In addition the verb "to consist" may be replaced by "to consist essentially of meaning that a product or a composition or a nucleic acid molecule or a peptide or polypeptide of a nucleic acid construct or vector or cell as defined herein may comprise additional component(s) than the ones specifically identified; said additional component(s) not altering the unique characteristic of the invention. In addition, reference to an element by the indefinite article "a" or "an" does not exclude the possibility that more than one of the elements is present, unless the context clearly requires that there be one and only one of the elements. The indefinite article "a" or "an" thus usually means "at least one".

[0074] The following examples are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in anyway.

Description of the figures [0075]

Fig. 1. Linear relationship of Ply2638 (SEQ ID NO: 21 encoded by SEQ ID NO: 44) activity against S. aureus SA2638/2854 cells in dependency of endolysin concentration. Assays were performed under standard conditions (PBS buffer pH 7.4, 120 mM sodium chloride) in photometric lysis assays. Maximum activity was determined from the first derivative of regression fits from sigmoidal lysis curves, calculated vwth SigmaPlot software. Error bars represent standard deviation calculated from technical triplicate experiments.

Fig. 2. Influence of divalent cations on lytic activity of Ply2638 (SEQ ID NO: 21, encoded by SEQ ID NO: 44). The enzyme was EDTA treated with subsequent substitution of metal ions by dialysis against MOPS buffer containing MgCI2, CaCl2, ZnCl2, CuCl2, C0CI2, or MnS04. Ply2638 dialyzed against MOPS buffer omitting EDTA treatment served as reference. Error bars represent standard deviation, calculated from technical triplicate experiments.

Fig. 3. Lytic activity of 50 nM Ply2638 (SEQ ID NO: 21, encoded by SEQ ID NO: 44) on S. aureus SA2638/2854 cells after lyophilization and reconstitution. Activity was measured in a turbidity reduction assay under standard conditions. Lyophilization buffer was taken as a control. The triple domain enzyme recovers full lytic activity after freeze-drying.

Fig. 4. Lytic activity of 50 nM M23-LST_Ami2638_CBD2638 (SEQ ID NO: 29, encoded by SEQ ID NO: 48) on S. aureus SA2638/2854 cells after lyophilization and reconstitution (indicated as lyophilized) as compared to freshly prepared M23-LST_Ami2638_CBD2638 (SEQ ID NO: 29, encoded by SEQ ID NO: 48) and Ply2638 (SEQ ID NO: 21, encoded by SEQ ID NO: 44). Lyophilization buffer was taken as a control. Activity was measured in a turbidity reduction assay under standard conditions. The triple domain enzyme recovers full lytic activity after freeze-drying.

Fig. 5. Relative activity of Lysostaphin (LST; SEQ ID NO: 34, encoded by SEQ ID NO: 33) and Ply2638 derivatives (SEQ ID NO: 31, 30, 29, 21 and 24, encoded by SEQ ID NO: 11, 10, 48, 44 and 4, respectively) in dependency of the concentration. The activity of LST at 50 nM was set as reference. All assays were done under standard conditions (37°C, pH 7.4 and 120 mM sodium chloride concentration) using S. aureus SA2638/2854 substrate cells from frozen stock.

Fig. 6. Relative activities at various pH values (left diagram) and sodium chloride concentrations (right diagram) of truncated and (with PlyTw domains) retrofitted Ply2638 variants determined in turbidity reduction assays. Truncation of the enzyme by one of the two catalytic domains resulted in impaired activities. Duplication of the CBD (M23_Ami_SH3b_SH3b; SEQ ID NO: 32, encoded by SEQ ID NO; 49) accelerates lysis at basic pH values and elevated salt concentrations. Retrofitting of Ply2638 with CHAP11 domain results in an enzyme which is presumed to attack three different bonds in the peptidoglycan layer of Staphylococcus. It shifted pH optima to slight acidic conditions and improved antibacterial activity. However, protein stability of the chimeric enzymes remains a challenge. Maximum lysis velocity of Ply2638 at standard conditions (pH 7.4 and 120 mM sodium chloride concentration) was set as reference. Bars represent mean of triplicate assays, standard deviation is not shown. (CHAP_M23_Ami_SH3b = SEQ ID NO: 25, encoded by SEQ ID NO: 45; Ami_M23_Ami_SH3b = SEQ ID NO: 26, encoded by SEQ ID NO: 46; M23_Ami_SH3b_SH3b = SEQ ID NO: 32, encoded by SEQ ID NO: 49; M23_Ami_SH3b = SEQ ID NO: 21, encoded by SEQ ID NO: 44; Ami_SH3b = SEQ ID NO: 22, encoded by SEQ ID NO: 2; M23_SH3b = SEQ ID NO: 23, encoded by SEQ ID NO: 3).

Fig. 7.: Activity of 50 nM quadruple domain enzymes (SEQ ID NO: 27 and 28, encoded by SEQ ID NO: 47 and 8, respectively), Ply2638 (SEQ ID NO: 21, encoded by SEQ ID NO: 44), and Lyosotaphin (SEQ ID NO: 34, encoded by SEQ ID NO: 33) against using S. aureus SA2638/2854 substrate cells under standard conditions. Lyophilization buffer was taken as a control. Quadruple domain enzymes were constructed by combining domains of Ply2638, PlyTw, and Lysostaphin.

Examples

Materials and Methods

Bacterial strains, culture conditions, phages and plasmids [0076] E. coli XLIBIueMRF and E. coli Sure was used for the over-expression of 6x-His-tagged (SEQ ID NO: 43) fusion proteins. Both strains were cultured in LB-PE medium at 30°C with 100 pg/ml ampicillin and 30 pg/ml tetracycline for plasmid selection. Phage 2638A lysate were used as template for the amplification of Ply2638A gene or domain coding regions thereof. CHAPTw domain (SEQ ID NO: 19) was amplified from Phage Twort lysate. Cystein- histidine dependent amidase/ peptidase (CHAP) domain and amidase domain from phage 11 (SEQ ID NO 18 and 17, respectively; Donovan, et al., 2006 and 2008; Navarre et al., 1999; Sass and Bierbaum 2007) were amplified from a pet21a vector containing phi11 autolysin gene, a kindly gift from Donovan, D.M.. Plasmid LT1215 containing the sequence of mature Lysostaphin (SEQ ID NO: 33) was used as template for domain amplification M23-LST (SEQ ID NO: 15) and CWT-LST (SEQ ID NO: 13). pQE-30 vector (catalogues number: 32915, Qiagen, Hlden, Germany; SEQ ID NO: 50) was used as cloning and expression vector for the production of 6x-His tagged recombinant fusion proteins in E. coli XL1 BlueMRF' or E. coli Sure respectively. DNA techniques and cloning procedures [0077] Standard techniques according to Sambrook, Maniatis et al. (1989) were employed for cloning of single genes and the creation of fusion proteins, high Fidelity PCR Enzyme mix (Fermentas) was used in PCR reactions. DNA concentrations were determined with a spectrophotometer (NanoDrop ND-1000 Spectrophotometer). pHP12638 is constructed by insertion of Ply2638 (SEQ ID NO: 1) encoding sequence Met1 - Lys486 into pQE30 (SEQ ID NO: 50) sites BamHI - Sail. pHP12638-P12638 construct has the same sequence consecutively inserted into BamHI -Sacl - Sail sites. CHAP11 (SEQ ID NO: 18), Ami11 (SEQ ID NO: 17) and CHAP_Ami11 were N-terminal introduced into BamHI digested pHPL2638A. Prior to ligation reaction, the vector was dephosphorylated using shrimp alkaline phosphatase (SAP, Fermentas). pHM23_CBD2638 (SEQ ID NO: 3), and pHM23-2638_Ami2638_CBD2638_CBD2638 (SEQ ID NO: 49) were constructed by a replacement of GFP coding region from pHGFP_CBD2638A-c vector (SEQ ID NO: 59) with the respective inserts using BamHI and Sacl restriction sites. pHM23-LST_Ami2638_CBD2638 (SEQ ID NO: 48) has a pQE-30 backbone with mature lysostaphin (SEQ ID NO: 33) coding sequence Alai - Gly154 inserted into BamHI and Sacl and Ply2638 partial sequence encoding Leu138 -Lys486 in Sacl and Sail sites. pHM23-LST_M23-LST_CWT-LST (SEQ ID NO: 11) has mature Lysostaphin (SEQ ID NO: 33) sequences Alai - Gly154 inserted into BamHI and Sacl and Alai - Lys246 into Sacl and Sail sites of pQE30. pHLST-LST (SEQ ID NO: 10) is constructed the same way having Alai - Lys246 repeatedly in BamHI - Sacl and Sacl - Sail sites. In plasmids encoding quadruple domain constructs pHCHAPTw_Ami2638_M23-LST_CBD2638 (SEQ ID NO: 47) and pHCHAPTw_Ami2638_M23- LST_CWT-LST (SEQ ID NO: 8) the domains are directly fused via splicing overlap extension PCR (SOE) and inserted into pQE30 BamHI and Sail sites. In both constructs, boarder regions of individual domains (CHAPTw, SEQ ID NO: 19: Met1 - Ile140, Ami2638, SEQ ID NO: 16: Lys141-Gly358 of SEQ ID NO: 1, CBD2638, SEQ ID NO: 12 : Trp393- Lys486 of SEQ ID NO: 1, M23-LST, SEQ ID NO: 15: Alai - Gly154 of SEQ ID NO: 33, CWT-LST, SEQ ID NO: 13: Trp155 - Lys246 of SEQ ID NO: 33) were determined with bioinformatics (unpublished data). Plasmids with repetitive sequences were transferred into E. coli Sure strain, all other plasmids into E. coli XLIBIueMRF'.

Expression and purification of recombinant fusion proteins [0078] Protein overexpression and partial purification was essentially done as previously described by others (Loessner et al., 1996, Schmelcher et al., 2010). In brief, plasmid bearing E. coli were grown in 250 ml modified LB medium (15 g/l tryptose, 8 g/l yeast extract, 5 g/l NaCI, pH 7.8) to an optical density at 600nm (OD600nm) of 0.4 to 0.6 and induced with 1 mM IPTG. Cells were further incubated for 4 hours at 30°C, or 18 hours at 20°C, cooled to 4°C, and harvested by centrifugation. Cell pellets were suspended in 5 ml immobilization buffer (50 mM NaH2P04, 500 mM NaCI, 5 mM imidazole, 0.1% polysorbate20, pH 7.4). Cytosolic E. coli contents containing soluble recombinant proteins were liberated by a double passage through a French Pressure Cell Press (1200 psi, SLM Aminco, Urbana, IL, U.S.) operated at 1200 psi. Other downstream processing steps included removal of insoluble cell debris by centrifugation, filter sterilization (0.2 pm PES membrane, Millipore), and Immobilized Metal Affinity Chromatography (IMAC) purification using MicroBiospin (Bio-Rad, Hercules, CA, U.S.) columns packet with low density Ni-NTA Superflow resin (Chemie Brunschwig AG, Basel, Switzerland). Ni-NTA immobilized proteins were on-column gravity flow washed with 5-10 column volumes immobilization buffer. Protein fractions were then eluted with elution buffer (50 mM NaH2P04, 500 mM NaCI, 125 mM imidazole, 0.1% polysorbate20, pH 7.4) and dialyzed against two changes of dialysis buffer (50 mM NaH2P04, 100 mM NaCI, 0.1% polysorbate20, pH 7.4). Protein concentrations were defined in a NanoDrop ND-1000 spectrophotmeter, corrected for specific absorbance at 280 nm as calculated from the primary amino acid sequence with Vector ΝΓΓΙ software (Invitrogen, Carlsbad, CA, U.S.) and estimated for purity by SDS-PAGE. Aliquots were stored at -20°C mixed with 50% glycerol.

Lyophilization of recombinant proteins [0079] IMAC purified proteins were dialyzed against 3 changes of 300 ml lyophilization buffer (50 mM phosphate or Tris, 500 mM sucrose, 200 mM mannitol, pH 7.4) aliquot and frozen in the gaseous phase of liquid nitrogen. The freeze-drying was done at -40°C and vacuum at 75 mTorr for 60 minutes, followed by increasing temperature during 5 hours to -10°C and another 60 minutes at -10°C at the same vacuum levels. As final step, temperature was increased to 25°C during 10 hours. Samples were reconstituted prior to testing in lysis assays by the addition of water.

Cell wall-binding assay [0080] As a standard assay to determine the ability of a CBD to direct a GFP fusion to the bacterial surface and mediate tight binding to the cell wall ligand, the following conditions is used: bacteria, preferably S. aureus BB255, from late log phase are harvested by centrifugation, resuspended in 1/10th volume of PBS-T (50 mM NaH2P04 120 mM NaCI [pH 8.0], 0.01% polysorbate 20) and stored on ice. GFP-CBD proteins, preferably SEQ ID NO: 64, encoded by SEQ ID NO: 60, are diluted in the same buffer to a concentration of 400 nM (2x GFP-CBD) and also stored on ice. In a 1.5 ml microcentrifuge cup, 100 pi cells and 100 μΙ of 2x GFP-CBD are mixed and incubated at room temperature for 5 min. Cells are then removed from the supernatant by centrifugation in a microfuge (16000 g, 60 s). The supernatant was discarded and cells were washed twice in 500 μΙ of PBS-T buffer. For fluorescence microscopy the pellet was finally resuspended in 50 μΙ of buffer. For fluorometer assays, the pellet is finally resuspended in 200 μΙ of PBS-T and transferred to a microplate well. Quantitative fluorescence assays can be performed using a multi label counter device (Victor3, Perkin Elmer, Massachusetts, U.S.) with sterile, untreated, black 96-well polystyrene microplates (Nunc, Roskilde, Denmark). As a negative control GFP can be used.

Quantitative fluorescence assays [0081] Dependency of pH and salt on CBD2638 to S. aureus BB255 cell surface ligand interaction is investigated by incubation of cells from 1 ml volume set to an OD600nm 1 +/- 0.05 (~4 x 10^ cells) with 7.5 pg GFP-CBDS2638 fusion protein, SEQ ID NO: 64, encoded by SEQ ID NO: 60. This cell to protein ratio is close to saturation point as determined in previous experiments and enables detection of variations in binding efficiencies. Varying pH is tested using citrate buffers pH 4.5 to 6.5 and phosphate buffers pH 6 to 9. After incubation with GFP-CBD2638 protein in pH buffer, cells are washed with respective pH buffer followed by standard PBS-T (pH 8) washing. Finally, cells are adjusted to an OD5g5nm = 0.3 to detect fluorescence from 200 μΙ suspensions thereof with a Victor3 multi label counter. Similar experiments are performed using buffers prepared with increasing sodium chloride concentrations (10 mM NaH2P04, 0 - 1000 mM NaCI, 0.1% polysorbate 20, pH 6).

[0082] Quantification of CBD binding capacity of Staphylococcus strains with altered cell surface properties is tested by recording relative fluorescence units (RFU) of washed and heat killed cells previously incubated with excessive GFP-CBD2638 protein. Fluorescence of equal volumes (200 μΙ) of GFP-CBD2638 labeled cells, adjusted to an OD595nm = 0.3, are measured using appropriate filter sets in a multi label counter device. Comparison and quantification of absorption levels of GFP-CBD2638 (SEQ ID NO: 64, encoded by SEQ ID NO: 60), GFP-CBD2638-CBD2638 (SEQ ID NO 65 and/or 66, encoded by SEQ ID NO: 61 and 62, respectively), and GFP-CBD2638-CBD2638-CBD2638 (SEQ ID NO: 67, encoded SEQ ID NO: 63) on S. aureus BB255 cells and SDS treated cells is done the same way.

Lysis assays [0083] Substrate cell for lytic activity assays were grown to an optical density at 600nm (OD600) of 0.4, washed twice with PBST pH 7.4 and re- suspended in 15% glycerol containing PBS buffer pH 7.4 concentrating it at the same time 100 fold. The cells were stored at -20°C. For further use in binding or lytic activity assays the cells were thaw, washed wth PBS pH 7.4 and diluted to an OD600 of 1 ± 0.05. In standard lytic activity assays protein samples were diluted to equimolar amounts and distributed in transparent 96-well tissue culture test plates (SPL life sciences, Pocheon, Korea). Substrate cells were added to a final volume and drop in optical density at 595nm (OD595nm) were recorded for about 1 hour at 37°C.

[0084] Lytic activity of retrofitted and deletion constructs of Ply2638 were tested against Phage 2638A propagation strain S. aureus SA2638/2854 from frozen stock in lysis assays. We tested the activity at various buffer conditions. pH values from 4.6 to 9 in 0.4 increments were tested using Citrate/ Phosphate buffers (25 mM Citrate, 25 mM Phosphate, 120 mM NaCI, pH 4.6 - 6.6) and Tris/ Phosphate buffers (25 mM Tris, 25 mM Phosphate, 120 mM NaCI). The activity of Ply2638A derivatives at salt concentrations ranging from 0 to 1000 mM Sodium chloride (in 10 mM phosphate buffer pH 7.4) was tested. The Ply2638A derivatives were diluted to 10 μΜ final concentration with MQ prior to its application in lysis assays. Here, 4 μΙ of 10 μΜ Ply2638A derivatives were applied to 196 μΙ substrate cell suspensions using a multichannel pipette, resulting in an assay concentration of 200 nM protein. The substrate cell suspensions were prepared from frozen stocks, diluting it with pH or salt buffers and standardizing it spectrophotemetrically (Libra S22, Biochrom) to an initial OD600 of 1 ± 0.05. Decrease in optical density at 595 nm (OD595) was measured using a Victor3 1420 Multilabel Counter instrument (Perkin Elmer) during 1 hour. Plates were shaken vigorously for 1 second (double orbit, 0.1mm diameter) after every single read out. As positive control served N-terminal 6xHis tagged Lysostaphin (HLST), commercially available Lysostaphin (recombinant, E. coli originated, Sigma). As negative control we applied MilliQ water.

Influence of divalent metal ions on the activity of Ply2638 [0085] Partially purified Ply2638 (SEQ ID NO: 21) was dialyzed for 2 hours against EDTA containing buffer (50 mM MOPS, 100 mM sodium chloride, 0.005% polysorbate20, 10 mM EDTA) followed by dialysis against buffer containing the respective divalent metal ions (50 mM MOPS, 100 mM sodium chloride, 0.005% polysorbate20, and 10 mM CaCI2, 10 mM MgCl2, 1 mM C0CI2, 1 mM CuCl2, 1 mM MnS04 or 1 mM ZnCl2 respectively). Cells used as substrate were SDS treated and EDTA washed prior to its application in standard lysis assays.

Tabel 1. SEQ ID NO identification

enzyme/domain/construct/vector nucleic amino acid I amino acid nucleic acid I acid sequence I sequence of sequence of His-sequence domain with tagged construct putative linker

Mature enzyme | SEQ ID NO: SEQ ID NO:1 j

Ply2638 1 21 SEQ ID NO: 44 enzyme/domain/construct/vector nucleic amino acid jamino acid nucleic acid j

I

on7umo/rlnmain/rnncfriirt/x/orfnr nurlair I aminA arirl 1 aminn arirl 3 nurloir ariH 5

t SEQ ID NO: 1 <Plv26381 [0086]

ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCT

GACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTAC

CATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTT

TTGATGTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTAC’TAGCGGAA

CAGTT ATT GAAGC AAACGATT ACGGT AATTTTGGT GGT ACATTCGTT ATT AG

AGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAAT

GCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACA

AGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACA

ATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTG

GTTTGGCT ATGGAAAA AT ATGACATT ACAAATTTA AATGCTAAACAAGAT A

AATCAAAGAAT GGGAGC GTGAAAG AGTT GAAAC AT AT CT ATT CAAACCAT

ATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTC

ATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTAT

ATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATG

CAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGC

ATT GT GGT AATC AAT GGGC AAATGCT AACTT AAT CGGATTT GAAGTGT GTG

AGTCGT ATCCTGGT AGAATCTCGGACAA ATT ATTCTTAGA AAATGAAGAAG

CGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTA

ATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACA

TCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAA

TATTAATAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGA

CGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACG

TTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACA

GATTGGAAACAGAAT AAAGAT GGC ATTTGGT AT AAAGCT GAAC ATGCTTCG

TTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGG

ACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATAT

GATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTT

GAGGGCGAAACTGT ATACATGCCGGT ACGCACAT GGGACGCTAAAACTGG

T AAAGTT GGT AAGTTGT GGGGCGAAATT AAAT AA SEQ ID NO: 2 (Ami2638 CBD2638 + 6xHis and cloning sites) [0087]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCTTACGCCCTAAAGAC

GCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAA

ATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGA

GCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGA

TT AC AGCAC C AAAAC CT AGT ATT C AAGGT GTGGTCATCC ACAAT GATT AT G

GTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATA

ACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAG

TGCTTT GGTAT CATCCGAC AGACT AC GT AGAGT GGCATT GT GGT AATC AAT

GGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTA

GAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAG

CTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTAC

GTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTT

GCATGTT GGC AAAGGT GAGCCTT AC AC AACTACT AAT ATT AAT AAAAT GAA

AGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGA

AGT A AGCAAAGCAGCAACT ATC A AACAATCTG ACGTTA AGCA AGA AGTT A

AAAAGCAAGAAGCAAAAC AAATTGT GAAAGCAACAGATT GGAAAC AGAAT

AAAGATGGCATTTGGT ATA AAGCT GAAC ATGCTTCGTTC ACAGTGACAGC A

CCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAA

GCTGGT GTATT ACAAAAAGGTC AAACGATTAAAT AT GAT GAGGTT C AAAAA

TTTGACGGTCATGTTT GGGTATCGTGGGA A ACGTTTGAGGGCG AAACT GT A

TACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTG

TGGGGCGAAATTAAATAA SEQ ID NO: 3 (M23-2638 CBD2638 + 6xHis and cloning sites) [0088]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATT GACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTAC GATGGTT ACCCTAA AAACT ACGGCT ACAGA AATT ACCATGAAAACGGCATT AATT AT GAT GAGTTTTGT GGTGGTT ATCAT AGAGCTTTTGATGTTT ACAGT A ACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAA ACG ATTACGGT AATTTTGGTGGTACATTCGTT ATT AG AGACGCT AACG AT A ACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAG GCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACT

ATTACGACAATCCTATGAGTGTACATTTACATTT ACAATTACGCCCT AAAGA

CGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAA

AAT AT GACATTAC AAATTTA AATGCT AAACAAG AT A AATCAAAGAATGGG

AGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAG

ATTACAGCACCAAAACCTAGTATTCAAGGTGAGCTCGGTGGAAAGCTAGAA

GT AAGC AAAGCAGC AACT ATCAAAC AAT CT GAC GTT AAGCAAGAAGTT AA

AAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATA

AAGAT GGC ATTT GGT AT AAAGCT GAAC AT GCTTCGTT C ACAGT GACAGC AC

CAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAG

CTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAAT

TTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTAT

ACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGT

GGGGCGAAATTAAATAA SEQ ID NO: 4 (Plv2638-Plv2638 + 6x His and cloning sites» [0089]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATT

GACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTAC

GATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATT

AATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTA

ACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAA

ACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATA

ACGATT GG AT AT AT GGGCATCT ACAACGT GGCT CAAT GCGATTT GTTGT AG

GCGACAAAGT CAAT CAAGGT GACATT ATTGGTTTAC AAGGTAAT AGC AACT

ATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCT AA AGA

CGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAA

AATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGG

AGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAG

ATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTAT

GGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAAT

AACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAA

GTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAA

T GGGCAAATGCT AACTT AATCGGATTTGAAGT GTGTGAGTCGT ATCCTGGT

AGAATCTCGGAC AAATT ATTCTT AG AAAAT GAAGAAGCGAC ATT GAAAGT A

GCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTA

CGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACT

TGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGA

AAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAG

AAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTT

AAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGA

AT A A AGAT GGC ATTT GGT AT A A AGCTGA AC ATGCTTCGTT CAC AGTGAC AG

CACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCAC

AAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAA

AATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTG

TATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGT

TGTGGGGCGAAATTAAAGAGCTCATGCTAACTGCTATTGACTATCTTACGA

AAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTA

AAAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGT

TTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGA

CGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAA

TTTTGGTGGTACATTCGTTATT AG AGACGCTA ACG AT AACG ATTGGATAT AT

GGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAAT

CAAGGTGACATTATTGGTTTACAAGGTAATAGCAACTATTACGACAATCCT

ATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGAT

GAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACA

AATTTAAATGCTAAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTT

GAAACATATCTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAA

ACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACC

TAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGT

TAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCA

TCCGAC AGACT ACGT AGAGT GGCATT GT GGT AAT C AATGGGCAAAT GCT AA

CTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAA

ATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGAT

GAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGA

ATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAA

GGTGAGCCTTACACAACTACTAATATTAATAAAATGAAAGACTACTTCATC

AAACGCATCAAACATTATTATGACGGTGGAAAGCTAGAAGTAAGCAAAGC

AGC AACTAT CAAAC AAT CT GACGTT AAGCAAGAAGTT AAAAAGC AAGAAG CAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATT T GGT AT AAAGCTG AACATGCTTCGTTCACAGTG ACAGCACC AGAGGGAATT ATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTA CAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCAT GTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTA CGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATT AAATAA SEQ ID NO: 5 (CHAP11 M23-2638 Ami2638 CBD2638) [0090]

ATGCAAGCA AAATTA ACT AAA AATGAGTTT AT AGAGTGGTTGAA AACTTCT

GAGGGAAAACAATT C AATGT GGACTT ATGGT ATGGATTT CAAT GCTTT GAT

TATGCCAATGCTGGTTGGAAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAG

GTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTAT

ACCAAAATACACCGGACTTCTTAGCACAACCTGGCGACATGGTGGTATTCG

GTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAA

CTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGA

CTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGA

CAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTG

AGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAA

ACAGCTGGATCCATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGG

AAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGC

TACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTTTTGTGGTGGT

T ATC ATAGAGCTTTT GAT GTTT ACAGT AACGAAACT AACGACGT GC CTGCT

GTTACTAGCGGAACAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGT

ACATTCGTTATTAGAGACGCTAACGATAACGATTGGATATATGGGCATCTA

CAACGTGGCTCAATGCGATTTGTTGTAGGCGACAAAGTCAATCAAGGTGAC

ATT ATTGGTTT ACAAGGTA ATAGCAACT ATTACGACA ATCCTATGAGTGT A

CATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCA

CAAGTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAAT

GCT AAACAAGAT AAAT C AAAGAATGGGAGCGTGAAAG AGTT GAAACAT AT

CTATTCAAACCATATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTAT

T CAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACCTAGTCAATA

CTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTG GGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGA CTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGG ATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTA GAAAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTA CGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGAATTCTTCGG AACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCT T ACACAACT ACTA AT ATT AAT AAAATGA AAGACT ACTTCATCAAACGCATC AAAC ATT ATT ATGACGGT GGAAAGCT AGAAGT AAGCAAAGC AGC AACT AT CAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAA TTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAA GCT GAAC ATGCTT C GTTC ACAGT GACAGC ACC AG AGGGAATTAT CAC AAGA T ACAAAGGT CCTT GGACT GGTC ACCC ACAAGCT GGTGT ATT AC AAAAAGGT CAAACGATT AAAT AT GAT GAGGTT C AAAAATTT GACGGT CAT GTTT GGGT A TCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGG GACGCT AAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA SEQ ID NO: 6 (Ami 11 M23-2638 Ami2638 CBD2638) [0091]

AAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAA

AGGTTATGACCTACCTAAGCGTGGTAGTAACCCTAAAGGTATAGTTATACA

CAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGAT

TAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACG

T ATCAGGCAACACAGTTT GGCAAGCCTT AGATGAATCACAAGT AGGTTGGC

ATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTC

AATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTT

TCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGA

AAT ACAAT C AGATT GC AC AAT GAATTTACTT CAAC ATC AT GC CCTC AT AGA

AGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAG

AAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGT

ACATGGATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTT

CAAGTAATACAGTTAAACCAGTTGCAAGTGCAGGATCCATGCTAACTGCTA

TTG ACT ATCTTACG AA AAA AGGTTGG A A AATATCATCTG ACCCTCGCACTT

ACGATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCA

TTA ATT ATGATGAGTTTTGTGGTGGTT ATC ATAGAGCTTTTGATGTTT ACAG

TAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTTATTGAAGC

AAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGA

T AACGATTGGAT AT AT GGGCAT CT AC AACGT GGCT C AAT GCGATTT GTTGT

AGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAA

CTATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAA

GAC GCAAAGAAAGAT GAAAAAT C AC AAGT AT GTAGTGGTTT GGCT AT GGA

AAAATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATG

GGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACA

AGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATT

ATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGA

ATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACG

AAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATC

AATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTG

GTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAG

TAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTG

TACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGA

CTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAAT

GAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCT

AGAAGT A AGC AAAGCAGCAACT ATCA AACA ATCTGACGTT AAGCA AGAAG

TT AAAAAGC AAGAAGC AAAAC AAATT GT GAAAGCAAC AGATTGGAAAC AG

AAT A AAGAT GGCATTTGGT AT AAAGCT GA ACAT GCTTCGTTCACAGTGACA

GCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCA

CAAGCT GGTGT ATT ACAAAAAGGT C AAACGATT AAAT AT GAT GAGGTTC AA

AAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACT

GT AT ACATGCCGGT ACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAG

TTGTGGGGCGAAATTAAATAA SEQ ID NO: 7 (CHAPTw Ami2638 M23-LST CBD2638) [0092]

ATG A AAACCCTG A AACAAGC AGAGT CCT ACATT AAG AGT AAAGTA AATAC AGGAACTGATTTTGATGGTTTATATGGGTATCAGTGTATGGACTTAGCAGT AGATT AT ATTT ACC ATGT AAC AGAT GGT AAAAT AAGAATGTGGGGT AAT GC TAAGGATGCGATAAATAACTCTTTTGGTGGTACTGCTACGGTATATAAAAA

CTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGT

AATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATG

GAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGA

TTT AT AAAACCGAGTT AGCT AC AAT C AGAACAC ACGATTAC AC AGGAATT A

CACATTTTATTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTG

GTTTGGCT ATGGA AAAATATGACATT AC A AATTTA AATGCT AA ACAAGAT A

AAT CAAAGAAT GGGAGC GT GAAAG AGTT GAAAC ATAT CTATT C AAACC AT

ATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTC

ATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTAT

ATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATG

CAAATAGAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGC

ATTGT GGTAATC A ATGGGCA AATGCT AACTTA ATCGGATTTGAAGTGTGT G

AGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAG

CGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTT A

ATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACA

TCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAA

T ATT AATA A AATGAAAGACT ACTTCATC A AACGCATCAA ACATT ATT ATGA

CGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACG

TTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACA

GATGCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAA

GGATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTAC

GGAGTTGATTTTTTT ATGA AT ATT GGAACACCAGT AAAAGCT ATTTC AAGC

GGAAA AAT AGTTGAAGCTGGTTGGAGTA ATTACGGAGGAGGT AATCAAAT

AGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAG

TAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGG

TTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGA

ATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAA

AGAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAAT

ACAGGTTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCT

T CGTTCACAGTGACAGCACC AGAGGGAATTATCACAAGATACAA AGGTCCT

TGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAA

TATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACG

TTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACT GGT AAAGTT GGT AAGTTGT GGGGC G AAATT AAAT AA SEQ ID NO: 8 (CHAPTw Ami2638 M23-LST CWT-LST +6xHis and cloning site) [0093]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAAAACCCTGAAA

CAAGCAGAGTCCTACATTAAGAGTAAAGTAAATACAGGAACTGATTTTGAT

GGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATG

TAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAAT

AACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGAC

CTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGG

TCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTT

ACAGTT CTTG AACAAAACTGGAACGGT AACGGG ATTT AT AAAAC CGAGTT A

GCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATTAAAGAC

GCAAAGAAAGAT GAAAAAT CAC AAGT AT GT AGTGGTTTGGCT AT GGAAAA

ATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGA

GCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAGA

TTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATG

GTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATA

ACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAG

TGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAAT

GGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTA

G AAT CT CGG AC AAATT ATT CTTAG AAAATG AAG AAG CG AC ATT G AAAGT AG

CT GCGGAT GTGATGAAGTCGT ACGGATT ACCAGTTAATCGCAACACTGTAC

GTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTT

GCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGAA

AGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGA

AGT AAGC AAAGCAGC AACT AT C AAACAATCT GACGTT AAGC AAGAAGTT A

AAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATGCTGCAACACAT

GAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGT

CCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTA

TGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAG

CT GGTTGGAGT AATT ACGGAGGAGGT AATC AAAT AGGTCTT ATT GAAAAT G

ATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAG

T AGGAGATTAT GT C AAAGCT GGT CAAAT AAT CGGTTGGTCT GGAAGC ACT G

GTT ATTCTACAGCACCAC ATTTACACTTCCA AAGAATGGTT AATTCATTTT C

AAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGG

AAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAA

ACA AATATGGCACACT AT AT AA ATCAGAGTCAGCT AGCTTCAC ACCTA AT A

CAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAG

GAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAG

ACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACT

TGCCTGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGG

AACT ATAAAGTAA SEQ ID NO: 9 (M23-LST Ami2638 CBD2638) [0094]

GCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGG

ATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGG

AGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGG

AAAAAT AGTTGAAGCTGGTTGGAGT AATT ACGGAGGAGGT AAT C AAAT AG

GT CTT ATTGAA A ATG AT GGAGTGCAT AGACA ATGGT AT ATGC ATCT AAGT A

AATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTT

GGTCT GGAAGC ACTGGTT ATT CT AC AGC ACC ACATTT ACACTT CCAAAGAA

TGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAA

GAGCGCAGGATATGGAAAAGCAGGTGGTACAGTAACTCCAACGCCGAATA

CAGGTGAGCTCTTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAA

GTATGTAGTGGTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCT

AAACAAGATAAATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTA

TTCAAACCATATTA AAGGTAACAAGATTACAGCACCAAAACCTAGTATTC A

AGGT GTGGT CAT CC ACAAT GATT AT GGT AGT AT GACACCTAGT CAAT ACTT

ACCATGGTTATATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGC

T AGTGTTTATGCAA AT AGAAACGAAGT GCTTTGGT ATCATCCGACAGACT A

CGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATT

TGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGA

AAATGAAGAAGCGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACG

GATT ACCAGTT AATCGCAACACTGT ACGTCTGCAT AACGA ATTCTTCGGAA

CTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAAGGTGAGCCTTA

CAC A ACT ACTA AT ATT A ΑΤΑ AAATGA A AGACT ACTTCATC A AACGCATCAA

ACATT ATT AT GACGGT GGAAAGCT AG AAGTAAGC AAAGC AGC AACT ATC A

AACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATT

GTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGC

TGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATA

CAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCA

AAC GATT AAAT ATGATGAGGTT C AAAAATTT GAC GGT C ATGTTT GGGT AT C

GTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGG

ACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAATAA SEQ ID NO: 10 (LST LST + 6xHis and cloning sites) [0095]

AT GAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAA

CATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCT

T ATCCATTAGGT ATAA ATGGCGGTATGCACTACGGAGTTGATTTTTTT AT GA

ATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTG

GTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATG

GAGTGCAT AGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAG

GAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTT

ATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAA

TTCA ACTGCCCA AGATCCA ATGCCTTTCTT AAAGAGCGCAGGAT ATGGAA A

AGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAAACAAACA

AAT AT GGCACACT AT ATAAATCAGAGTCAGCTAGCTTCACACCTAATACAG

ATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAGTCAGGAG

TCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAACAAGACG

GTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAACGTATTTACTTGCC

TGTAAGAACATGGAATAAATCTACTAATACTTTAGGTGTTCTTTGGGGAAC

TATAAAGGAGCTCGCTGCAACACATGAACATTCAGCACAATGGTTGAATAA

TTACAA AAAAGGAT ATGGTT ACGGTCCTT ATCCATTAGGT AT AA ATGGCGG

ATTTCAAGCGGAAAAAT AGTTGA AGCTGGTTGGAGT AATTACGGAGGAGGT AATCAAATAGGTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATG CATCTAAGTAAATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAA ATAATCGGTTGGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACT

TCCAAAGAATGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCC TTTCTT AAAGAGCGCAGGAT AT GGAAAAGCAGGT GGT AC AGT AACTCC AAC GCC GAATAC AGGTT GGAAAACAAACAAATAT GGCACACT AT AT AAATCAG AGTCAGCTAGCTTCACACCTA AT ACAGAT AT AAT AACA AGAACGACTGGTC CATTTAGAAGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTC ATTATGATGAAGTGATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAG GTA ACAGT GGCCAACGT ATTT ACTTGCCTGTAAGA ACATGGA AT AAATCT A CT AAT ACTTTAGGTGTTCTTT GGGGAACT AT AAAGT GA SEQ ID NO: 11 (M23-LST M23-LST CWT-LST +6xHis and cloning sites) [0096]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAA

CATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCT

TATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGA

AT ATTGGAAC ACCAGT AAAAGCT ATTT CAAGC GGAAAAAT AGTTGAAGCT G

GTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATG

GAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAG

GAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTGGTT

ATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAA

TTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAA

AGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTGAGCTCGCTGCAAC

ACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTA

CGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTT

TTT ATGAAT ATTGGAACACCAGTAAAAGCT ATTTCAAGCGGAAA A ATAGTT

GAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAA

AATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTT

AAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGC

ACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCAT

TTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGAT

AT GGAAAAGC AGGT GGT AC AGT AACTCCAACGCCGAAT ACAGGTTGG AAA

ACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTCACACCT

AATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATGCCGCAG

TCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTGATGAAA

CAAGACGGT CAT GTTTGGGT AGGTT ATACAGGTAACAGTGGCCAACGT ATT

T ACTTGCCTGT AAGAACATGGA ATA A ATCTACT AATACTTTAGGTGTTCTTT GGGGAACTΑΤΑAAGTGA SEQ ID NO: 12 (CBD-2638) [0097]

T GG A AACAGAAT AAAG ATGGCATTTGGT ATA A AGCTGAACATGCTTCGTTC

ACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGAC

TGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGA

T GAGGTT CAAAAATTT GACGGT CAT GTTT GGGTAT CGT GGGAAACGTTT GA

GGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTA

AAGTTGGTAAGTTGTGGGGCGAAATTAAATAA SEQ ID NO: 13 (CWT-LST) [0098]

TGGAAAACAAACAAATATGGCACACTATATAAATCAGAGTCAGCTAGCTTC ACACCTAATACAGATATAATAACAAGAACGACTGGTCCATTTAGAAGCATG CCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGATGAAGTG ATGAAACAAGACGGTCATGTTTGGGTAGGTTATACAGGTAACAGTGGCCAA CGT ATTT ACTTGCCTGT AAGA ACAT GG AAT AAATCT ACTA AT ACTTT AGGTG TTCTTTGGGGAACTATAAAGTGA SEQ ID NO: 14 (M23-2638) [0099]

ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCT GACCCTCGCACTT ACGATGGTT ACCCT AAAAACT ACGGCT ACAGA AATTAC CATG AAA ACGGCATTAATT AT GAT GAGTTTT GTGGTGGTT ATCATAG AGCTT TTGAT GTTT AC AGT AACGAAACT AACGACGTGC CTGCT GTT ACT AGCGGAA CAGTT ATTG AAGCAAACGATT ACGGT AATTTTGGTGGT ACATTCGTT ATT AG AGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAAT GCGATTT GTT GTAGGCGAC AAAGTC AATC AAGGT GAC ATT ATTGGTTT AC A AGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACA ATTACGCCCTAAAGACGCAAAGAAAGAT SEQ ID NO: 15 (M23-LS~n [0100]

GCT GCAACAC ATGAACATT CAGC ACAAT GGTT GAAT AATT AC AAAAAAGG ATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGG

AGTT GATTTTTTT AT GAAT ATTGG AACACC AGT AAAAGCTATTT C AAGCGG

AAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAG

GTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTA

AAT AT AATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTT

GGTCTGGAAGCACTGGTTATTCTACAGCACCACATTTACACTTCCAAAGAA

TGGTTAATTCATTTTCAAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAA

GAGCGCAGGATAT SEQ ID NO: 16 (Ami-26381 [0101]

GGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCAC

AATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCAC

GTG AGA AT AACGGT ACACACGTTAACGGTTGGGCTAGTGTTT ATGCAAAT A

GAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTG

GTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGT

ATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACAT

TGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCA

ACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTC

GTGGGACTTGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAA

TAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGT SEQ ID NO: 17 (Ami-<D111 [0102]

AAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAA

AGGTTATGACCT ACCT AAGCGTGGT AGT AACCCTAAAGGTATAGTTATACA

CAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGAT

T AGT AAATGCACCTTT ATCAAGATTAGA AGCGGGCATTGCGCATAGTTACG

TATCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGC

ATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTC

AATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTT

TCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGA

AATACAATCAGATTGCACAATGAATTTACTTCAACATCATGCCCTCATAGA

AGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAG

AAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGT

ACATGGATGGT AAA ATACCGGTTGCCACTGTCTCT AATGAGTCA AGCGCTT

C AAGT AATACAGTT AAACC AGTT GC AAGT GC A SEQ ID N0:18 (CHAP-<D111 [0103]

ATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCT

GAGGGAAAACAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGAT

T ATGCC AATGCTGGTT GGAAAGTTTT GTTT GGATT ACTT CTAAAAGGTTT AG

GTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTAT

ACC A AAAT ACACCGGACTTCTT AGCACAACCTGGCGACATGGTGGT ATTCG

GTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAA

CTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGA

CTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGA

CAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTG

AGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAA

ACAGCT SEQ ID NO:19 (CHAP-OTwortl [0104]

AT GA AAACCCTGAAACAAGCAGAGT CCT ACATTA AGAGTAA AGTA A ATAC AGGAACTGATTTT GAT GGTTT AT ATGGGT AT CAGT GT AT GGACTT AGC AGT AGATTAT ATTT ACCAT GT AACAGATGGT AAAAT AAGAATGTGGGGTA AT GC T AAGGATGCGAT AAAT AACTCTTTTGGTGGT ACTGCT ACGGT AT ATA AAA A CTACCCTGCTTTTAGACCTAAGTACGGTGATGTAGTCGTATGGACTACTGGT AATTTTGCAACTTATGGTCATATCGCAATAGTTACTAACCCTGACCCTTATG GAGACCTTCAATATGTTACAGTTCTTGAACAAAACTGGAACGGTAACGGGA TTT ATAAAACCGAGTTAGCTACAATCAGAACACACGATTACACAGGAATTA CACATTTTATT SEQ ID NQ:20 (M23-2638 Ami2638 CBD2638 CBD26381 [0105]

ATGCTAACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCT GACCCTCGCACTTACGATGGTTACCCTAAAAACTACGGCTACAGAAATTAC CATG AAA ACGGCATTA ATT ATGATGAGTTTTGTGGTGGTT AT CAT AGAGCTT TTGAT GTTT AC AGT AACGAAACT AACGACGTGC CTGCT GTT ACT AGCGGAA CAGTTATTGAAGCAAACGATTACGGTAATTTTGGTGGTACATTCGTTATTAG AGACGCTAACGATAACGATTGGATATATGGGCATCTACAACGTGGCTCAAT

GCGATTT GTT GT AGGCG ACAAAGTC AATCAAGGT GAC ATT ATT GGTTT AC A

AGGTAATAGCAACTATTACGACAATCCTATGAGTGTACATTTACATTTACA

ATTACGCCCTAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTG

GTTTGGCTATGGAAAAATATGACATTACAAATTTAAATGCTAAACAAGATA

AATCAAAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCAT

ATTAAAGGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTC

ATCCACAATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTAT

ATGCACGTGAGAATAACGGTACACACGTTAACGGTTGGGCTAGTGTTTATG

CAAATAGAAAC GAAGT GCTTTGGT AT CATCCGAC AGACT ACGT AGAGT GGC

ATTGTGGTAATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTG

AGTCGTATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAG

CGACATTGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTA

ATCGCAACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACA

T CGTTCGTGGGACTTGCAT GTTGGCAA AGGTGAGCCTTACACAACT ACT AA

T ATT AATA A AATGAA AGACT ACTTCATCAA ACGCATCAAACATT ATT ATGA

CGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACG

TTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACA

GATTGGA AACAGA AT AAAGATGGCATTTGGT ATA AAGCTG A ACATGCTTCG

TTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGG

ACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATAT

GATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTT

GAGGGCGAAACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGG

T AAAGTT GGT AAGTT GT GGGGCGAAATT AAAGAGCTCGGT GGAAAGCT AG

AAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTT

AAAAAG CAAG AAG CAAAAC AAATT GT G AAAGC AAC AG ATT G G AAACAG A

AT AAAGAT GGCATTT GGTATAAAGCTGAACATGCTT CGTTCACAGTGACAG

CACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCAC

AAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAA

AATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTG

TATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGT

TGTGGGGCGAAATTAAATAA SEQ ID NO: 21 (Plv26381 [0106]

MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGI

NYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDN

DWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKD

AKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITA

PKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLW

YHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADV

MKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNTNKMKDYFI

KRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIW

YKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVW

VS WETFEGETVYMPVRTWD AKT GKV GKLW GEIK SEQ ID NO: 22 (Ami2638 CBD26381 [0107]

MRGSHHHHHHGST.RPKDAKKDEKSOVCSGT AMF.KYDTTNT NAKODKSKNG

SVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGT

HVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRIS

DKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVG

KGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAK

QIVKATDWKQNKDGIWYKAEHASFTVTAPEGUTRYKGPWTGHPQAGVLQKG

QTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK SEQ ID NO: 23 (M23-2638 CBD26381 [0108]

MRGSHHHHHHGSMT.TATnYTTKKGWKTSSnPRTYDGYPKNYGYRNYHF.NGT

NYDEFCGGYHRAFDVYSNETNDVPAVTSGTVTEANDYGNFGGTFVTRDANDN

DWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKD

AKKDEKSQVCSGLAMEKYDTTNLNAKQDKSKNGSVKELKHTYSNHTKGNKTTA

PKPSIQGELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIW

YKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVW

VS WETFEGETVYMPVRTWD AKT GKV GKLW GEIK SEQ ID NO: 24 (Plv2638-Plv26381 [0109]

MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGI

NYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDN

DWIYGHLQRGSMRFWGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKD

AKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITA

PKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLW

YHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADV

MKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNTNKMKDYFT

KRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIW

YKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVW

VSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKELMLTAIDYLTKKGWKISS

DPRT YDGYPKNY GYKNYFIEN GINYDEF C GGYFIRAFDVY SNETNDVP AVTS GT

VTEANDYGNFGGTFVIRDANDNDWTYGHLQRGSMRFVVGDKVNQGDITGLQG

NSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKS

KNGSVKELKHIYSNHIKGNKITAPKPSIQGVVIHNDYGSMTPSQYLPWLYAREN

NGTHVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANLTGFEVCESYP

GRISDKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDL

HVGKGEPYTTTNINKMKDYF1KRIKHYYDGGKLEVSKAAT1KQSDVKQEVKK

QEAKQIVKATD WKQNKDGIWYKAEFIASFTVT APEGIITRYKGP WT GHPQAGV

LQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWG

EIK SEQ ID NO: 25 (CHAP11 M23-2638 Ami2638 CBD2638) [0110]

MRGSHHHHHHGSMO AKT TKNEFTEWT KTSEGKOFNVDT ,WY GFOCFDY AN A

GWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYG

AGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYD

FPMWFIRPNFKSETAPRSVQSPTQAPKKETAGSMLTAIDYLTKKGWKISSDPRT

YDGYPKNYGYRNYHENGINYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEA

NDYGNFGGTFVIRDANDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNY

YDNPMSVHLHLQLRPKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGS

VKELKHTYSNHTKGNKTTAPKPSTQGWTHNDYGSMTPSQYLPWLYARENNGTH

VN GWAS VY ANRNEVLWYHPTDYVE WHC GN Q WAN ANLIGFEV CES YPGRISD

KLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGK

GEPYTTTNINKMKDYF1KR1KHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQ

IVKATDWKQNKDG1WYKAEHASFTVTAPEG1ITRYKGPWTGHPQAGVLQKGQ

TIKYD EVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK SEQ ID NO: 26 IAmi11 M23-2638 Ami2638 CBD26381 [0111]

MRGSHHHHHHGSKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSK

GATAEAYRNGLVNAPLSRLEAGTAHSYVSGNTVWQALDESQVGWHTANQTGN

KYYY GIE V CQ SMGADNATFLKNEQ ATF QECARLLKKW GLPANRNTIRLUNEF

TSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVS

NESSASSNTVKPVASAGSMLTATDYLTKKGWKTSSDPRTYDGYPKNYGYRNYH

EN GINYDEFCGGYFIRAFDVY SNETND VPAVTSGTVIE ANDY GNF GGTFVIRD A

NDNDWIYGHLQRGSMRFVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLR

PKDAKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGN

KITAPKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNE

VLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVA

ADVMKSYGLPVNRNTVRLFTNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKD

YFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDG

IWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHV

WVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK SEQ ID NO: 27 (CHAPTw Ami2638 M23-LST CBD26381 [0112]

MRGSHHHHHHGSMKTLKQAESYIKSKVNTGTDFDGLY GY QCMDLAVDYIY

HVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVVVWTTGNFAT

YGHIAIVTNPDPYGDLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFItCDA

KKDEKLSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAP

KPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWY

HPTDYVEWHCGNQWANANLIGFEVCESYPGRTSDKLFLENEEATLKVAADVM

KSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKR

IKHYYDGGKEEVSKAATIKQSDVKQEVKKQEAKQIVKATDAATHEHSAQWLN

NYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKA1SSGK1VEAGWSNYGGG

NQJGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQI1GWSGSTGYSTAPHLHF

QRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKQNKDGIWYKAE

HASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWE

TFEGETVYMPVRTWDAKTGKVGKLWGEIK SEQ ID NO: 28 (CHAPTw Ami2638 M23-LST CWT-LST1 [0113]

MRCSHHHHHHCSMKTLKQAESYTKSKVNTGTDFDGLYGYQCMDLAVDYTY

HVTDGKIRMWGNAKDAINNSFGGTATVYKNYPAFRPKYGDVVVWTTGNFAT YGHIAIVTNPDPYGDLQYVTVLEQNWNGNG1YKTELATIRTHDYTGITHF1KDA KKDEKS QV C SGLAMEKYDITNLN AKQDKSKNGS VKELKHIY SNHIKGNKIT AP KPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWY HPTDYVEWHCGNQWANANLTGFEVCESYPGRTSDKLFLENEEATLKVAADVM KSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFIKR IKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDAATHEHSAQWLN NYKKGY GY GPYPLGINGGMFIY GVD FFMNIGTPVKAIS S GKIVEAGW SNY GGG NQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHF QRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSES ASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSG 0R1YLPVRTWNKSTNTEGVLWGTIK SEQ ID NO: 29 (M23-LST Ami2638 CBD2638) [0114]

MRr.SHHHHHHr.SAATHF.HSAOWTNNYKKGYGYGPYPTGTNGGMHYr.VDFF

MNTGTPVKATSSGKTVEAGWSNYGGGNQTGLTENDGVHRQWYMHLSKYNVKV

GDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGK

AGGTVTPTPNTGELLRPKDAKKDEKSQVCSGLAMEKYD1TNLNAKQDKSKNG

SVKELKHIYSNHIKGNKITAPKPSIQGWIHNDYGSMTPSQYLPWLYARENNGT

HVNGWASVYANRNEVLWYHPTDYVEWHCGNQWANANL1GFEVCESYPGRIS

DKLFLENEEATLKVAADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVG

KGEPYTTTNINKMKDYFIKRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAK

QIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKG

QTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIK SEQ ID NO: 30 (LST LST1 [0115]

MRGSHHHHHHGSAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFF

MNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKV

GDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGK

AGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTD1ITRTTGPFRSMPQSGVLKA

GQTTHYDEVMKQDGHVWVGYTGNSGQRTYLPVRTWNKSTNTLGVLWGTTKE

LAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSG

KIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKA.GQIIGW

SGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTG WKTNKYGTLYKSESASFTPNTD1ITRTTGPFRSMPQSGVLKAGQT1HYDEVMK QDGFTVWV GYTGN SGQRIYLPVRTWNKSTNTLGVLWGTIK SEQ ID NO: 31 (M23-LST M23-LST CWT-LST) [0116]

MRG SHHHHHHG SAATHEHS AQ WLNNYKKGY GY GP YPLGINGGMHY GVDFE

MNTGTPVKATSSGKTVEAGWSNYGGGNQTGLTENDGVHRQWYMHLSKYNVKV

GDYVKAGQTTGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGK

AGGTVTPTPNTGELAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDF

FMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVK

VGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYG

KAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLK

AGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK

SEQ ID NO: 32 (M23-2638 Ami2638 CBD2638 CBD2638T

[0117]

MRGSHHHHHHGSMLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGI

NYDEFCGGYHRAFDVYSNETNDVPAVTSGTVIEANDYGNFGGTFVIRDANDN

DWTYGHLQRGSMRFVVGDKVNQGDTTGLQGNSNYYDNPMSVHLHLQLRPKD

AKKDEKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITA

PKPSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLW

YHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADV

MKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKDYFI

KRIKHYYDGGKLEVSKAATIKQSDVKQEVKKQEAFCQIVKATDWKQNKDGIW

YKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVW

VSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKELGGKLEVSKAATIKQSDV

KQEVKKQEAKQTVKATDWKQNKDGTWYKAEHASFTVTAPEGTTTRYKGPWTG

HPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWDAKTGK

VGKLWGEIK SEQ ID NO: 33 (LST) [0118]

GCTGCAACACATGAACATTCAGCACAATGGTTGAATAATTACAAAAAAGG ATATGGTTACGGTCCTTATCCATTAGGTATAAATGGCGGTATGCACTACGG AGTTGATTTTTTTATGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGG

AAAAATAGTTGAAGCTGGTTGGAGTAATTACGGAGGAGGTAATCAAATAG GTCTTATTGAAAATGATGGAGTGCATAGACAATGGTATATGCATCTAAGTA AATATAATGTTAAAGTAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTT GGTCT GGAAGCACTGGTT ATT CT AC AGC ACC AC ATTT AC ACTT CC AAAGAA T GGTT AATTC ATTTT CAAATT C A ACT GCCC AAGATCC AAT GCCTTTCTT AAA GAGCGCAGGAT ATGG A AA AGCAGGTGGT ACAGTA ACTCCA ACGCCG AAT A CAGGTT GGAAAAC AAACAAAT AT GGC ACACT AT ATAAAT CAGAGT C AGCT AGCTTCACACCT AATACAGAT AT AATA AC AAGAACGACTGGTCCATTT AGA AGCATGCCGCAGTCAGGAGTCTTAAAAGCAGGTCAAACAATTCATTATGAT GAAGT GAT GAAACAAGACGGT CATGTTT GGGTAGGTTATACAGGTAACAGT GGCC AACGT ATTTACTTGCCTGT AAGA ACATGGAATA AATCT ACT AAT ACT TTAGGTGTTCTTTGGGGAACTATAAAGTGA SEQ ID NO: 34 (LST aal [0119]

AATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMN1GTPVKA1SSGK 1VEAGWSNYGGGNQIGL1ENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWS GSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTGW KTNKY GTLYKSESASFTPNTDIITRTT GPFRSMPQSGVLKAGQTIHYDEVMKQD GHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGTIK SEQ ID NO: 35 (CBD-26381 [0120]

WKQNKDGTWYKAEHASFTVTAPEGTTTRYKGPWTGHPQAGVLQKGQTIKYDE V OKFDGFTVWV S WETFEGETVYMPVRT WDAKT GKV GKL W GEIK SEQ ID NO: 36 (CWT-LST) [0121]

WKTNKYGTLYKSESASFTPNTDTTTRTTGPFRSMPQSGVLKAGQTTHYDEVMK

QDGHVWVGYTGNSGQRJYLPVRTWNKSTNTLGVLWGTIK SEQ ID NO: 37 (M23-2638) [0122]

MLT AID YLTKKG WKIS SDPRTYDGYPKNY GYRNYHEN GINYDEF CGGYHRAF DVYSNETNDVPAVTSGTVTEANDYGNFGGTFVTRDANDNDWTYGHLQRGSMR FVVGDKVNQGDI1GLQGNSNYYDNPMSVHLHLQLRPKDAKKD SEQ ID NO: 38 (M23-LS~n [0123]

AATHEHSAQWLNNYICKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGK

IVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWS

GSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGY SEQ ID NO: 39 (Ami-26381 [0124]

NKJTAPKPSIQGVVIHNDYGSMTPSQYLPWEYARENNGTHVNGWASVYANRN

EVLWYHPTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATEKV

AADVMKSYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNTNKMK

DYFIKRIKHYYDG SEQ ID NO: 40 (Ami-(D111 [0125]

NPKGTVTFINDAGSKGATAEAYRNGLVNAPLSRLEAGTAFISYVSGNTVWQALD

ESQVGWHTANQTGNKYYYGTEVCQSMGADNATFLKNEQATFQECARLLKKW

GLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYF1KQI

RAYMD SEQ ID NO: 41 (CHAP-<D111 [0126]

MSI1MEVATMQAKLTKNEF1EWLKTSEGKQFNVDLWYGFQCFDYAMAGWKV

LFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMWFGSNYGAGYG

HVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMW

FIRP SEQ ID NO: 42(CHAP-<DTwort1 [0127]

MKTLKQAESYTKSKVNTGTDFDGLYGYQCMDLAVDYTYHVTDGKTRMWGNA KDAINN SFGGT ATVYKNYPAFRPKY GD VVVWTT GNFAT Y GHIAIVTNPDPY G DLQYVTVLEQNWNGNGTYKTELATTRTHDYTGTTHFT SEQ ID NO 43 (6xHis tag N terminal)

[0128] MRGSHHHHHHGS SEQ ID NO: 44 (Plv2638 + 6xHis and cloning sites) [0129]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATT

GACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCCTCGCACTTAC

GATGGTTACCCTAAAAACTACGGCTACAGAAATTACCATGAAAACGGCATT

AATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTA

ACGAAACT AACGACGTGCCTGCTGTT ACT AGCGG AACAGTT ATTGA AGCA A

ACGATT ACGGT AATTTTGGT GGT AC ATTCGTT ATT AGAGAC GCT AACGAT A

ACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAG

GCGACAAAGTC AATCAAGGTGAC ATT ATT GGTTT AC AAGGT AAT AGC AACT

ATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGA

CGC A AAGA AAGATGA A A AATC ACAAGT AT GTAGTGGTTT GGCT ATGGAAA

AATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGG

AGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAG

ATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTAT

GGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAAT

AACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAA

GTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAA

TGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGT

AGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTA

GCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTA

CGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACT

TGCATGTTGGCAAAGGTGAGCCTTACACAACTACTA AT ATT AATA AAATGA

AAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAG

AAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTT

AAAAAGC AAGAAGCAAAACAAATT GT GAAAGCAACAGATTGGAAACAGA

ATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAG

CACCAGAGGGAATT ATCACAAGAT ACAAAGGTCCTTGGACTGGTCACCCAC

AAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAA

AATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTG

TATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGT

TGTGGGGCGAAATTAAATAA SEQ ID NO: 45 (CHAP 11 M23-2638 Ami2638 CBD2638+6xHis and cloning sites) [0130]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCAAGCAAAATTA ACT AAAAAT GAGTTT AT AG AGT GGTT GAAAACTT CT GAGGGAAAAC AATTC

AATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTT

GGAAAGTTTT GTTT GGATT ACTTCTAAAAGGTTTAGGT GCAAAAGAT ATTCC

GTTCGCTAACAACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGA

CTTCTTAGCACAACCTGGCGACATGGTGGTATTCGGTAGCAACTACGGTGC

TGGATATGGTCACGTTGCATGGGTAATTGAAGCAACTTTAGATTACATCATT

GTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACGGAATCGAACA

ACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTATGATTT

CCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATC

AGTTCAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTGGATCCATGCT

AACTGCTATTGACTATCTTACGAAAAAAGGTTGGAAAATATCATCTGACCC

TCGCACTT ACGATGGTTACCCT AAAAACT ACGGCT ACAGA A ATT ACCATGA

AAACGGCATTAATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGAT

GTTTACAGTAACGAAACTAACGACGTGCCTGCTGTTACTAGCGGAACAGTT

ATT GAAGC AAAC GATT AC GGT AATTTT GGT GGTAC ATT CGTTATTAGAGAC

GCT AACGATAACGATTGGAT AT ATGGGCATCTACAACGTGGCTCAATGCGA

TTTGTTGTAGGCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGT

AAT AGCAACT ATTACGACA ATCCTAT GAGTGT ACATTT ACATTT AC A ATT AC

GCCCT AAAGAC GCAAAGAAAGAT GAAAAAT CAC AAGTAT GT AGT GGTTTG

GCT AT GG ΑΑΑΑΑΤ AT GAC ATTACAAATTT AAAT GCTAAACAAGATAAATC A

AAGAATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAA

GGTAACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCAC

AATGATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCAC

GTGAGAAT AACGGTACACACGTTA ACGGTTGGGCTAGTGTTT ATGCAAAT A

GAAACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTG

GT AAT CAATGGGCAAAT GCT AACTT AAT CGGATTT GAAGT GTGT GAGT CGT

ATCCTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACAT

TGAAAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCA

ACACTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTC

GT GGGACTTGC ATGTT GGC AAAGGT G AGC CTTACAC AACT ACT AAT ATT AA

TAAAATGAAAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGG

AAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGC

AAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGG

AAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACA

GTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGT CACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAG GTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGC GAA ACTGT ATACATGCCGGT ACGCACAT GGGACGCT AAAACTGGTA AAGTT GGT AAGTTGT GGGGCG AAATT AAAT AA SEQ ID NO: 46 (Ami11 M23-2638 Ami2638 CBD2638+6xHis and cloning sites) [0131]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCAAGCCACAACCTAAA

GCAGTAGAACTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCT

AAGCGTGGTAGTAACCCTAAAGGTATAGTTATACACAACGACGCAGGGAG

CAAAGGGGCG ACT GCT GAAGCAT ATCGT AACGG ATT AGTAAATGC ACCTTT

ATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTATCAGGCAACACAGT

TTGGCAAGCCTTAGATGAATCACAAGT AGGTTGGCAT ACCGCTAATCAAAT

AGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGA

TAACGCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAG

ATTGTTGAAAAAATGGGGATTACCAGCAAACAGAAATACAATCAGATTGC

ACAATGAATTTACTTCAACATCATGCCCTCATAGAAGTTCGGTTTTACACAC

TGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAGAAGACAAGCGGTTGCA

ACTT AAAGACT ACTTT AT C AAGC AG ATT AGGGCGT AC AT GGAT GGT AAAAT

ACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAA

ACCAGTTGCAAGTGCAGGATCCATGCTAACTGCTATTGACTATCTTACGAA

AAAAGGTTGGAAAATATCATCTGACCCTCGCACTTACGATGGTTACCCTAA

AAACTACGGCTACAGAAATTACCATGAAAACGGCATTAATTATGATGAGTT

TTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTAACGAAACTAACGAC

GTGCCTGCTGTT ACT AGCGGAAC AGTT ATTGAAGC AAACGATT ACGGT AAT

TTTGGTGGT ACATTCGTT ATT AGAGACGCT AACG AT AACGATTGGATAT AT

GGGCATCT ACA ACGTGGCTCA ATGCGATTTGTTGT AGGCGACAA AGTCAAT

CAAGGTGAC ATT ATT GGTTTAC AAGGT AAT AGC AACT ATT ACGAC AATCCT

ATGAGTGTACATTTACATTTACAATTACGCCCTAAAGACGCAAAGAAAGAT

GAAAAATCAC AAGT ATGT AGT GGTTTGGCT AT GGAAAAATAT GAC ATTACA

AATTT AAATGCT AAACAAG AT AAAT CAAAG AAT GGGAGCGT G AAAG AGTT

GAAACAT AT CT ATT CA AACCAT ATT AAAGGT AACA AG ATT ACAGCACCA A A

ACCT AGT ATTCAAGGTGTGGTCATCCACAATGATTATGGTAGTATGACACC

TAGTCAATACTTACCATGGTTATATGCACGTGAGAATAACGGTACACACGT

TAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAGTGCTTTGGTATCA

TCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAATGGGCAAATGCTAA

CTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTAGAATCTCGGACAA

ATTATTCTT AGAAAAT GAAGAAGCG AC ATT GAAAGT AGCTGC GGATGT GAT

GAAGTCGTACGGATTACCAGTTAATCGCAACACTGTACGTCTGCATAACGA

ATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTTGCATGTTGGCAAA

GGT GAGCCTT ACAC AACT ACTAAT ATT AAT AAAATGAAAGACTACTT CAT C

AAACGCATC AAACATT ATT ATGACGGTGGA AAGCT AGAAGTA AGCA AAGC

AGC AACTAT CAAAC AAT CT GACGTT AAGCAAGAAGTT AAAAAGC AAGAAG

CAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATT

TGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATT

ATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTA

CAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCAT

GTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTA

CGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATT

AAAT AA SEQ ID NO: 47 (CHAPTw Ami2638 M23-LST CBD2638 +6xHis and cloning sites) [0132]

AT GAGAGGATCGCATCACCATCACCATCACGGATCCATGAAAACCCTGAAA

CAAGCAGAGTCCTAC ATT AAGAGT AAAGT AAATACAGGAACTGATTTT GAT

GGTTTATATGGGTATCAGTGTATGGACTTAGCAGTAGATTATATTTACCATG

TAACAGATGGTAAAATAAGAATGTGGGGTAATGCTAAGGATGCGATAAAT

AACTCTTTTGGTGGTACTGCTACGGTATATAAAAACTACCCTGCTTTTAGAC

CTAAGTACGGTGATGTAGTCGTATGGACTACTGGTAATTTTGCAACTTATGG

TCATATCGCAATAGTTACTAACCCTGACCCTTATGGAGACCTTCAATATGTT

ACAGTTCTTGAACAAAACTGGAACGGTAACGGGATTTATAAAACCGAGTTA

GCTACAATCAGAACACACGATTACACAGGAATTACACATTTTATTAAAGAC

GCAAAGAAAGAT GAAAAAT CAC AAGT AT GT AGTGGTTTGGCT AT GGAAAA

ATATGACATTACAAATTTAAATGCTAAACAAGATAAATCAAAGAATGGGA

GCGTG AAAG AGTT GAAAC ATAT CT ATT C AAACC AT ATT AAAGGT AAC AAGA

TTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTATG

GTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAATA

ACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAAG

TGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTAATCAAT

GGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGTA

G AAT CT CGGAC AAATT ATT CTTAGAAAATGAAGAAGCGAC ATT G AAAGT AG

CTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTAC

GTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACTT

GCATGTTGGCAAAGGTGAGCCTT ACACA ACTACT AAT ATT AAT AAA ATGA A

AGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAGA

AGT A AGCAAAGCAGCAACT ATC A AACAATCTGACGTTA AGCA AGA AGTT A

AAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATGCTGCAACACAT

GAACATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGT

CCTTATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTA

TGAATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAG

CT GGTTGGAGT AATT ACGGAGGAGGT AATC AAAT AGGTCTT ATT GAAAAT G

ATGGAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAG

TAGGAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTGGAAGCACTG

GTTATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTC

AAATTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGG

AAAAGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTTGGAAACAGA

ATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAG

CACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCAC

AAGCTGGT GT ATT ACA AAA AGGTCAAACGATT AAAT ATGATGAGGTTCAAA

AATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTG

TATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGT

TGTGGGGCGAAATTAAATAA SEQ ID NO: 48 (M23-LST Ami2638 CBD2638 + 6xHis and cloning sites) [0133]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCGCTGCAACACATGAA CATTCAGCACAATGGTTGAATAATTACAAAAAAGGATATGGTTACGGTCCT TATCCATTAGGTATAAATGGCGGTATGCACTACGGAGTTGATTTTTTTATGA ATATTGGAACACCAGTAAAAGCTATTTCAAGCGGAAAAATAGTTGAAGCTG GTTGGAGTAATTACGGAGGAGGTAATCAAATAGGTCTTATTGAAAATGATG GAGTGCATAGACAATGGTATATGCATCTAAGTAAATATAATGTTAAAGTAG

GAGATTATGTCAAAGCTGGTCAAATAATCGGTTGGTCTQGAAGCACTGQTT

ATTCTACAGCACCACATTTACACTTCCAAAGAATGGTTAATTCATTTTCAAA

TTCAACTGCCCAAGATCCAATGCCTTTCTTAAAGAGCGCAGGATATGGAAA

AGCAGGTGGTACAGTAACTCCAACGCCGAATACAGGTGAGCTCTTACGCCC

TAAAGACGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTA

T GGA AA A ATATGACATT ACA AATTT AAATGCT AA ACAAGATA A ATCAAAG

AATGGGAGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGT

AACAAGATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAAT

GATTATGGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTG

AGAATA ACGGT ACACACGTT AACGGTTGGGCT AGTGTTT ATGCA A AT AGAA

ACGAAGTGCTTTGGTATCATCCGACAGACTACGTAGAGTGGCATTGTGGTA

ATCAATGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATC

CTGGTAGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGA

AAGTAGCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACA

CTGTACGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTG

GGACTT GC AT GTT GGC AAAGGT GAGCCTT AC AC AACT ACT AAT ATT AAT AA

AATGAA AGACT ACTTCATC AAACGCATC A AAC ATTATT ATGACGGTGGAAA

GCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAG

AAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAA

CAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTG

ACAGCACCAG AGGG AATT ATCAC AAG ATACAAAGGTCCTT GG ACTGGTCAC

CC ACAAGCTGGT GT ATT AC AAAAAGGT C AAAC GATT AAAT ATGAT GAGGTT

CAAAAATTT GAC GGT CAT GTTT GGGT AT CGTGGGAAAC GTTTGAGGGCGAA

ACTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGT

AAGTTGTGGGGCGAAATTAAATAA SEQ ID NO: 49 (M23-2638 Ami2638 CBD2638 CBD2638 + 6xHis and cloning sites) [0134]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGCTAACTGCTATT GACT AT CTT ACGA AAAAAGGTTGGA AAAT AT CAT CTGACCCTCGCACTT AC GATGGTT ACCCTAA AAACT ACGGCT ACAGA AATT ACCATGAAAACGGCATT AATTATGATGAGTTTTGTGGTGGTTATCATAGAGCTTTTGATGTTTACAGTA ACGAAACT AAC GAC GTGCCTGCTGTTACT AGCGGAAC AGTT ATTGAAGC AA

ACGATTACGGTAATTTTGGTGGTACATTCGTTATTAGAGACGCTAACGATA

ACGATTGGATATATGGGCATCTACAACGTGGCTCAATGCGATTTGTTGTAG

GCGACAAAGTCAATCAAGGTGACATTATTGGTTTACAAGGTAATAGCAACT

ATTACGACAATCCTATGAGTGTACATTTACATTTACAATTACGCCCTAAAGA

CGCAAAGAAAGATGAAAAATCACAAGTATGTAGTGGTTTGGCTATGGAAA

AAT AT GAC ATTAC AAATTT AAAT GCTAAAC AAGAT AAATC AAAGAAT GGG

AGCGTGAAAGAGTTGAAACATATCTATTCAAACCATATTAAAGGTAACAAG

ATTACAGCACCAAAACCTAGTATTCAAGGTGTGGTCATCCACAATGATTAT

GGTAGTATGACACCTAGTCAATACTTACCATGGTTATATGCACGTGAGAAT

AACGGTACACACGTTAACGGTTGGGCTAGTGTTTATGCAAATAGAAACGAA

GTGCTTTGGT ATCATCCGACAGACTACGT AGAGTGGCATTGTGGT AATCAA

TGGGCAAATGCTAACTTAATCGGATTTGAAGTGTGTGAGTCGTATCCTGGT

AGAATCTCGGACAAATTATTCTTAGAAAATGAAGAAGCGACATTGAAAGTA

GCTGCGGATGTGATGAAGTCGTACGGATTACCAGTTAATCGCAACACTGTA

CGTCTGCATAACGAATTCTTCGGAACTTCTTGTCCACATCGTTCGTGGGACT

TGCATGTTGGCAAAGGTGAGCCTTACACAACTACTAATATTAATAAAATGA

AAGACTACTTCATCAAACGCATCAAACATTATTATGACGGTGGAAAGCTAG

AAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTT

AAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGA

ATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAG

CACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCAC

AAGCTGGT GT ATT ACAAAAAGGTC AAACGATT AAATAT GAT GAGGTTCAAA

AATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTG

TATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGT

TGTGGGGCGAAATTAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAAGCA

GCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGAAGC

AAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCATTT

GGT ATAAAGCT GAACAT GCTTC GTT C AC AGTGAC AGCACC AGAGGGAATT A

TCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTAC

AAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATG

TTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTAC

GCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTA

A AT A A SEQ ID NO: 50 (pQE-30 vector, available under cat. No.: 32915 at Qiaaen - Hilden. Germany) [0135]

CTCGAGAAATCAT AA A AAATTT ATTTGCTTTGTGAGCGGATAACAATT AT A

ATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAG

GAGAAATTAACTATGAGAGGATCGCATCACCATCACCATCACGGATCCGCA

TGCGAGCTCGGTACCCCGGGTCGACCTGCAGCCAAGCTTAATTAGCTGAGC

TTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTT

GTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAG

CTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAA

ATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACAT

TTTGAGGCATTTCAGTCAGTTGCTCAATGTACCT ATA ACCAGACCGTTCAGC

TGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTT

AT CCGGCCTTT ATTCACATTCTTGCCCGCCTGAT GA AT GCTCAT CCGGA ATT

TCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCC

TTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGT

GAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTG

GCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAAT

ATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAA

ACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAAT

ATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATC

ATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAAC

AGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATT

GGTGCCCTT AAACGCCT GGGGT AAT GACT CTCT AGCTT GAGGCAT CAAAT A

AAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGT

CGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGAGCTGCCTCGC

GCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGAC

GGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGG

GCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCAC

GT AGCGAT AGCGGAGT GT AT ACT GGCTTAACTAT GCGGCATCAGAGC AGAT

TGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAG

GAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTG

CGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTA

ATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGC

AAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT

TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA

GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC

CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA

CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGC

TGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC

ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCT

TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG

TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA

GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGC

TCTGCTGAAGCCAGTT ACCTTCGGA A A A AGAGTTGGTAGCTCTTGATCCGG

CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGAT

TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG

GT CTG AC GCT CAGT GGAAC GAAAACT C ACGTT AAGGGATTTT GGT CAT GAG

ATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTT

AAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGC

TTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAG

TTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCAT

CTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAG

ATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGT

CCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTA

GAGT AAGT AGTT CGCC AGTTAAT AGTTT GCGCAAC GTT GTTGCC ATTGCTAC

AGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGT

TCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCG

GTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGT

TATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATC

CGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGA

ATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAA

TACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTC

TTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGAT

GTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGC

GTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAAT

AAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTAT

TGAAGCATTT ATCAGGGTTATTGTCTCATGAGCGGAT ACAT ATTTGA ATGT A TTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGC CACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATA GGCGT ATCACGAGGCCCTTTCGTCTTCAC SEQ ID NO: 51 (CBD-2638 with putative linker indicated in bolch [0136]

GKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASF

TVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEG

ETVYMPVRTWDAKTGKVGKLWGEIK SEQ ID NO: 52 (CWT-LST with putative linker indicated in bold) [0137]

GKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGV

LKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWNKSTNTLGVLWGT

IK SEQ ID NO: 53 (M23-2638 with putative linker indicated in bolch [0138]

MLTAIDYLTKKGWKISSDPRTYDGYPKNYGYRNYHENGINYDEFCGGYHRAF DVYSNETNDVPAVTSGTVTEANDYGNFGGTFVTRDANDNDWTYGHLQRGSMR FVVGDKVNQGDIIGLQGNSNYYDNPMSVHLHLQLRPKDAKKDEKSQVCSGL AMEKYDITNLNAKQDKSKNGSVKELKHIY SNHIKG SEQ ID NO: 54 (M23-LST with putative linker indicated in bold) [0139]

AATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMMGTPVKAISSGK

IVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWS

GSTGYSTAPHLHFQRMVNSFSNSTAQDPMPFLKSAGYGKAGGTVTPTPNTG SEQ ID NO: 55 (Ami-2638 with putative linker indicated in bold) [0140]

EKSQVCSGLAMEKYDITNLNAKQDKSKNGSVKELKHIYSNHIKGNKITAPK

PSIQGVVIHNDYGSMTPSQYLPWLYARENNGTHVNGWASVYANRNEVLWYH

PTDYVEWHCGNQWANANLIGFEVCESYPGRISDKLFLENEEATLKVAADVMK

SYGLPVNRNTVRLHNEFFGTSCPHRSWDLHVGKGEPYTTTNINKMKBYFIKRI

KHYYDGCKLEVSKAATIKQSDVKQEVKKQEAKQIVKATD SEQ ID NO: 56 (Ami-ΦΙΙ with putative linker indicated in bold) [0141]

PKKETAKPQPKAVELKIIKDWKGYDLPKRGSNPKGIVIHNDAGSKGATAE

AYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYY GIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSC PHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSA S SNTVKPVASA SEQ ID NO: 57 (CHAP-(D11 with putative linker indicated in bold) [0142]

MSIIMEVATMQAKLTKNEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKV

LFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMWFGSNYGAGYG

HVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMW

FIRPNFKSETAPRSVQSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRG SEQ ID NO: 58 (CHAP-OTwort with putative linker indicated in bold) [0143]

MKTLKQAESYIKSKVNTGTDFDGLYGYQCMDLAVDYIYHVTDGK1RMWGNA KDAINNSFGGTATVYKNYPAFRPKY GDVVVWTT GNFATY GHIAIVTNPDPY G DLQYVTVLEQNWNGNGIYKTELATIRTHDYTGITHFIRPNFATESSVKKKDTK KKPKPSNRDGINKDKIVYDRTNINYNMVKRG SEQ ID NO: 59 (pHGFP CBD2638 c vector) [0144]

CTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATA AT AG ATTCAATTGTGAGCGGATA ACAATTT CACACAGA ATTCATT AAAGAG GAGAAATTAACTATGAGAGGATCGCATCACCATCACCATCACGGATCCATG AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTA GATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGT GATGCAACATACGGA AAACTT ACCCTT A AATTT ATTTGCACTACTGGAAAA CTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAAT GCTTT GCG AGATACCCAG ATCAT AT GA A ACGGCATG ACTTTTTCAAGAGT G CCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACG GGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTA AT AGAAT CGAGTT AAAAGGT ATT GATTTT AAAGAAGATGGAAAC ATTCTT G GACACAAATTGGAAT ACAACTATAACTCACACAATGTATACATCATGGCAG ACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATT

GAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATT

GGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTG

CCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGT

TTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAAGAGC

TCGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGAC

GTTAAGCAAGAAGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAAC

AGATTGGAAACAGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTC

GTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTG

GACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATA

TGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTT

TGAGGGCGAA ACTGT AT ACATGCCGGT ACGCACATGGGACGCTAAAACTG

GT AAAGTTGGT AAGTTGTGGGGCGAAATTAAAT AAGTCGACCTGCAGCCAA

GCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAG

AACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT

TGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTA

AAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGC

ATCGTAAAGAACATTTT GAGGCATTTCAGTCAGTT GCTCA ATGTACCT ΑΤΑ A

CCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAA

TAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAAT

GCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGG

GATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTT

CATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATAT

ATTCGCAAGATGTGGCGTGTTACGGT GAAAACCTGGCCT ATTTCCCT AAAG

GGTTT ATTGAG A AT ATGTTTTTCGTCT CAGCCA ATCCCT GGGTG AGTTTCAC

CAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTC

ACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCG

ATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTA

AAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTG

AGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTT

ATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTA

GAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCA

GCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACA

AGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCAT

GACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCA

TCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCAC

AGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTC

ACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCAC

TCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAA

GAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG

CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA

ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATAC

CAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC

CGCTT ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTC

TCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG

CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCC

GGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTG

GCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGC

TACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGT

ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGG

TAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT

TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAlAGATCCTTT

GATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGG

GATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAAT

T AAAAAT GAAGTTTT AAATCAAT CT AlAAGT AT AT ATGAGTAAACTT GGTCT

GACAGTT ACCA ATGCTT AATCAGTGAGGCACCT ATCTCAGCGAT CTGTCT AT

TTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACG

GGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACG

CTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGA

GCGCAGAAGT GGT CCT GCAACTTTATCCGCCT CCATCCAGT CTATTAATTGT

T GCCGGGAAGCT AGAGTAAGT AGTTCGCC AGTT AAT AGTTT GCGC AACGTT

GTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTT

CATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTT

GTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAA

GTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTT

ACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCA

AGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGT

CAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCA

TTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGA

GATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTT

TACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGC

AAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCT

TTTT CAAT ATTATTGAAGCATTT ATC AGGGTT ATT GT CT CAT G AGCGGAT AC

ATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTT

CCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTA

ACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC SEQ ID NO: 60 (GFP CBD26381 [0145]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGA

AGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTT

AATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATAC

GGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCAT

GGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATA

CCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGG

TTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGAC

ACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTT

AAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGA

ATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGA

ATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGC

GTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCT

GTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAG

ATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTG

CTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAG

CT AGAAGT AAGCAAAGCAGCAACT AT CA AACAATCTGACGTTA AGCA AGA

AGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAAC

AGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGA

CAGC ACCAG AGGGAATT ATCACA AG ATACA AAGGT CCTTGGACTGGTCACC

CACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTC

AAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAA

CT GT ATACATGCCGGT ACGCACATGGGACGCTAAAACTGGTAAAGTTGGTA AGTTGT GGGGCGAAATTAAATAA SEQ ID NO: 61 (GFP CBD2638 CBD2638 Variant 1. Restriction sites used for construction: BamHI - Sacl - Sacl- Sail. TAAStop codon derived from 2— CBD2638> [0146]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGQAGA

AGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTT

AATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATAC

GGAAAACTT ACCCTT AA ATTT ATTTGCACT ACTGGAA A ACT ACCTGTTCC AT

GGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATA

CCCAGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGG

TTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGAC

ACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTT

AAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGA

ATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGA

ATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGC

GTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCT

GTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAG

ATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTG

CTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAG

CTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGA

AGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAAC

AGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGA

CAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACC

CACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTC

AAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAA

CTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTA

AGTTGTGGGGCGAAATTAAAGAGCTCGGTGGAAAGCTAGAAGTAAGCAAA

GCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGA

AGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCA

TTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAA

TTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTAT

TACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTC

ATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGG

TACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAA

ATTAAATAAGTCGAC SEQ ID NO: 62 (GFP CBD2638 CBD2638 Variant 2. Restriction sites used for construction: BamHI - Sacl - Kpnl - Sail. Stop codon is encoded bv vector) [0147]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGQAGA

AGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTT

AATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATAC

GGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCAT

GGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATA

CCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGG

TTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGAC

ACGTGCTGA AGTCAAGTTTGA AGGTGAT ACCCTT GTT A AT AG A AT CG AGTT

AAAAGGT ATTGATTTT A AAGA AGATGGAAACATTCTTGGAC AC A A ATT GG A

ATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGA

ATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGC

GTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCT

GT CCTTTTACCAGACAACC ATTACCT GTCC AC ACAATCTGCCCTTT C GAAAG

ATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTG

CTGGGATTACACATGGCATGGATGAACTATACAAAGAGCTCGGTGGAAAG

CT AGAAGT AAGCAAAGCAGCAACT ATCA AACAATCTGACGTT A AGCA AGA

AGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAAC

AGAATAAAGATGGCATTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGA

CAGCACCAGAGGGAATTATCACAAGATACAAAGGTCCTTGGACTGGTCACC

CACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTC

AAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAA

CTGTAT ACATGCCGGT ACGC ACATGGGACGCT AAAACTGGT AA AGTTGGT A

AGTTGTGGGGCGAAATTAAAGGTACCGGTGGAAAGCTAGAAGTAAGCAAA

GCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGA

AGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCA

TTTGGTATAAAGCTGAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAA

TTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTAT

TACAAAAAGGTCAAACGATTAAATATGATGAGGTTCAAAAATTTGACGGTC ATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGG T ACGCACAT GGGACGCTAAAACT GGTAAAGTT GGT AAGTTGT GGGGCGAA ATTAAAGTCGACCTGCAGCCAAGCTTAATTAGCTGA SEQ ID NO: 63 (GFP CBD2638 CBD2638 CBD2638. Restriction sites used for construction: BamHI - Sacl - Kpnl- Sail -Pstl. Stop codon is encoded bv vector) [0148]

ATGAGAGGATCGCATCACCATCACCATCACGGATCCATGAGTAAAGGAGA

AGAACTTTTC ACT GGAGTT GTCC CAATT CTTGTT GAATT AGAT GGT GAT GTT

AATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATAC

GGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCAT

GGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATA

CCCAGATCAT ATGAA ACAGCAT GACTTTTTCA AG AGTGCCAT GCCCG AAGG

TTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGAC

ACGT GCT GAAGT CAAGTTTGAAGGT GAT ACC CTT GTTAAT AGAAT CGAGTT

AAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGA

ATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGA

ATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGC

GTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCT

GTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAG

ATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTG

CT GGGATTACACATGGCAT GGATGAACTAT ACAAAGAGCT CGGTGGAAAG

CTAGAAGTAAGCAAAGCAGCAACTATCAAACAATCTGACGTTAAGCAAGA

AGTTAAAAAGCAAGAAGCAAAACAAATTGTGAAAGCAACAGATTGGAAAC

AGAAT AAAGATGGCATTTGGT AT AAAGCT GAAC ATGCTT CGTT CACAGT GA

CAGC ACCAG AGGGAATT ATCACA AG ATACA AAGGT CCTTGGACTGGTCACC

CACAAGCTGGTGTATTACAAAAAGGTCAAACGATTAAATATGATGAGGTTC

AAAAATTTGACGGTCATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAA

CTGTATACATGCCGGTACGCACATGGGACGCTAAAACTGGTAAAGTTGGTA

AGTTGT GGGGCGAAATT AAAGGT ACC GGT GGAAAGCT AGAAGT AAGC AAA

GCAGCAACTATCAAACAATCTGACGTTAAGCAAGAAGTTAAAAAGCAAGA

AGCAAAACAAATTGTGAAAGCAACAGATTGGAAACAGAATAAAGATGGCA

TTTGGT AT AAAGCTGA ACATGCTTCGTTCACAGTGACAGCACCAGAGGGAA

TTATCACAAGATACAAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTAT

T ACA AA A AGGTC AAACGATTA A AT ATGATGAGGTTCA AAA ATTTGACGGTC

ATGTTTGGGTATCGTGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGG

TACGCACATGGGACGCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAA

ATTAAAGTCGACGGTGGAAAGCTAGAAGTAAGCAAAGCAGCAACTATCAA

ACAATCTGACGTT AAGC AAGAAGTTAAAAAGCAAGAAGC AAAAC AAATT G

T GA A AGCAAC AGATT GGAAACAGAATA AAGATGGCATTTGGT ATAA AGCT

GAACATGCTTCGTTCACAGTGACAGCACCAGAGGGAATTATCACAAGATAC

AAAGGTCCTTGGACTGGTCACCCACAAGCTGGTGTATTACAAAAAGGTCAA

ACGATTAAATATGATGAGGTTCAAAAATTTGACGGTCATGTTTGGGTATCG

TGGGAAACGTTTGAGGGCGAAACTGTATACATGCCGGTACGCACATGGGAC

GCTAAAACTGGTAAAGTTGGTAAGTTGTGGGGCGAAATTAAACTGCAGCCA

AGCTT AATT AGCTGA SEQ ID NO: 64 (GFP_CBD2638 aa) [0149]

MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY

GKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFK.SAMPEGY

YQERTTFFKDDGNYKTRAEVKFEGDTLVNRTELKGTDFKEDGNTLGHKLEYNYN

SHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH

YT STOS AT SKDPNF.KRDHMVT ,T F.FVTA AGTTHGMDF.T .YKF.T ,GGKT .F.VSK A ATT

KQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYK

GPWTGHPQAGVLQKGQT1KYDEVQKFDGHVWVSWETFEGETVYMPVRTWD

AKTGKVGKLWGETK SEQ ID NO: 65 (GFP_CBD2638_CBD2638 Variant 1 aa) [0150]

MRGSHHHHHHGSMSKGFFI .FTGVVPIFVEFOGDVNGHKFSVSGF G EGDATY

GKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHDFFKSAMPEGY

VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN

SHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH

YL STQ S AL SKDPNEKRDHMVLLEFVT AAGITHGMDELYKEL GGKLEVSKAATI

KQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGIITRYK

GPWTGHPQAGVLQKGQTTKYDEVQKFDGTTVWVSWETFEGETVYMPVRTWD

AKTGKVGKLWGEIKELGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDW

KQNKDGIWYKAEHASFTVTAPEGIITRYKGPWTGHPQAGVLQKGQTIKYDEV

QKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGETK SEQ ID NO: 66 (GFP_CBD2638_CBD2638 Variant 2 aa) [0151]

MRGSHHHHHHGSMSKGEELFTGWPILVELDGDVNGHKFSVSGEGEGDATY

GKLTLKFTCTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGY

VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGMLGHKLEYNYN

STTNVYTMADKQKNGTKVNFKTRTTNTEDGSVQLADHYQQNTPTGDGPVLLPDNH

YL STQ S ALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATI

KQSDVKQEVKKQEAKQIVKATDWKQNKDGTWYKAEHASFTVTAPEGTTTRYK

GPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPVRTWD

AKTGKVGKLWGEIKGTGGKLEVSKAATIKQSDVKQEVKKQEAKQIVKATDW

KQNKDG1WYKAEHASFTVTAPEGT1TRYKGPWTGHPQAGVLQKGQTIKYDEV

QKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKVDLQPSLIS SEQ ID NO: 67 (GFP_CBD2638_CBD2638_CBD2638 aa) [0152]

MRGSHHHHHHGSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY

GKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGY

VQERTIFFKDDGNYKTRAEVKFEGDTLVNRiELKGIDFKEDGNILGHKLEYNYN

SHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH

YLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKELGGKLEVSKAATI

KQSDVKQEVKKQEAKQJVKATDWKQNKDG1WYKAEHASFTVTAPEG1ITRYK

GPWTGHPQAGVLQKGQT1KYDEVQKFDGHVWVSWETFEGETVYMPVRTWD

AKTGKVGKLWGEIKGTGGKLEVSKAATIKQSDVKQEVKKQEAKQIVBCATDW

KQNKDGTWYKAEHASFTVTAPEGITTRYKGPWTGHPQAGVLQKGQTTKYDEV

QKFDGHVWVSWETFEGETVYMPVRTWDAKTGKVGKLWGEIKVDGGKLEVS

KAATIKQSDVKQEVKKQEAKQIVKATDWKQNKDGIWYKAEHASFTVTAPEGI

ITRYKGPWTGHPQAGVLQKGQTIKYDEVQKFDGHVWVSWETFEGETVYMPV

RT WDAKT GKV GKLW GEIKLQPSLIS

SEQUENCE LISTING

[0153] <110> EBI Food Safety B.V. <120> A polypeptide <130> P6035159PCT <160> 67 <170> Patentln version 3.3

<210> 1 <211 > 1461 <212> DNA <213> Staphylococcus aureus bacteriophage 2638a <400> 1 atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc 60 acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat 120 tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac 180 gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt 240 ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt 300 ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa 360 ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct 420 aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat 480 gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg 540 aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt 600 caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg 660 ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat 720 agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa 780 tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg 840 gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag 900 tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact 960 tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact 1020 aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga 1080 aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa 1140 aagcaagaag caaaacaaat tgtgaaagca acagattgga aacagaataa agatggcatt 1200 tggtataaag ctgaacatgc ttcgttcaca gtgacagcac cagagggaat tatcacaaga 1260 tacaaaggtc cttggactgg tcacccacaa gctggtgtat tacaaaaagg tcaaacgatt 1320 aaatatgatg aggttcaaaa atttgacggt catgtttggg tatcgtggga aacgtttgag 1380 ggcgaaactg tatacatgcc ggtacgcaca tgggacgcta aaactggtaa agttggtaag 1440 ttgtggggcg aaattaaata a 1461 <210> 2 <211>1086 <212> DNA <213> artificial <220> <223> His-tagged Ami2638_CBD2368 <400>2 atgagaggat cgcatcacca tcaccatcac ggatccttac gccctaaaga cgcaaagaaa 60 gatgaaaaat cacaagtatg tagtggtttg gctatggaaa aatatgacat tacaaattta 120 aatgctaaac aagataaatc aaagaatggg agcgtgaaag agttgaaaca tatctattca 180 aaccatatta aaggtaacaa gattacagca ccaaaaccta gtattcaagg tgtggtcatc 240 cacaatgatt atggtagtat gacacctagt caatacttac catggttata tgcacgtgag 300 aataacggta cacacgttaa cggttgggct agtgtttatg caaatagaaa cgaagtgctt 360 tggtatcatc cgacagacta cgtagagtgg cattgtggta atcaatgggc aaatgctaac 420 ttaatcggat ttgaagtgtg tgagtcgtat cctggtagaa tctcggacaa attattctta 480 gaaaatgaag aagcgacatt gaaagtagct gcggatgtga tgaagtcgta cggattacca 540 gttaatcgca acactgtacg tctgcataac gaattcttcg gaacttcttg tccacatcgt 600 tcgtgggact tgcatgttgg caaaggtgag ccttacacaa ctactaatat taataaaatg 660 aaagactact tcatcaaacg catcaaacat tattatgacg gtggaaagct agaagtaagc 720 aaagcagcaa ctatcaaaca atctgacgtt aagcaagaag ttaaaaagca agaagcaaaa 780 caaattgtga aagcaacaga ttggaaacag aataaagatg gcatttggta taaagctgaa 840 catgcttcgt tcacagtgac agcaccagag ggaattatca caagatacaa aggtccttgg 900 actggtcacc cacaagctgg tgtattacaa aaaggtcaaa cgattaaata tgatgaggtt 960 caaaaatttg acggtcatgt ttgggtatcg tgggaaacgt ttgagggcga aactgtatac 1020 atgccggtac gcacatggga cgctaaaact ggtaaagttg gtaagttgtg gggcgaaatt 1080 aaataa 1086 <210> 3 <211> 1035 <212> DNA <213> artificial <220> <223> His-tagged M23-2638_CBD2638 <400>3 atgagaggat cgcatcacca tcaccatcac ggatccatgc taactgctat tgactatctt 60 acgaaaaaag gttggaaaat atcatctgac cctcgcactt acgatggtta ccctaaaaac 120 tacggctaca gaaattacca tgaaaacggc attaattatg atgagttttg tggtggttat 180 catagagctt ttgatgttta cagtaacgaa actaacgacg tgcctgctgt tactagcgga 240 acagttattg aagcaaacga ttacggtaat tttggtggta cattcgttat tagagacgct 300 aacgataacg attggatata tgggcatcta caacgtggct caatgcgatt tgttgtaggc 360 gacaaagtca atcaaggtga cattattggt ttacaaggta atagcaacta ttacgacaat 420 cctatgagtg tacatttaca tttacaatta cgccctaaag acgcaaagaa agatgaaaaa 480 tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540 caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600 aaaggtaaca agattacagc accaaaacct agtattcaag gtgagctcgg tggaaagcta 660 gaagtaagca aagcagcaac tatcaaacaa tctgacgtta agcaagaagt taaaaagcaa 720 gaagcaaaac aaattgtgaa agcaacagat tggaaacaga ataaagatgg catttggtat 780 aaagctgaac atgcttcgtt cacagtgaca gcaccagagg gaattatcac aagatacaaa 840 ggtccttgga ctggtcaccc acaagctggt gtattacaaa aaggtcaaac gattaaatat 900 gatgaggttc aaaaatttga cggtcatgtt tgggtatcgt gggaaacgtt tgagggcgaa 960 actgtataca tgccggtacg cacatgggac gctaaaactg gtaaagttgg taagttgtgg 1020 ggcgaaatta aataa 1035 <210> 4 <211 >2961 <212> DNA <213> artificial <220> <223> His-tagged Ply2638_Ply2638 <400>4 atgagaggat cgcatcacca tcaccatcac ggatccatgc taactgctat tgactatctt 60 acgaaaaaag gttggaaaat atcatctgac cctcgcactt acgatggtta ccctaaaaac 120 tacggctaca gaaattacca tgaaaacggc attaattatg atgagttttg tggtggttat 180 catagagctt ttgatgttta cagtaacgaa actaacgacg tgcctgctgt tactagcgga 240 acagttattg aagcaaacga ttacggtaat tttggtggta cattcgttat tagagacgct 300 aacgataacg attggatata tgggcatcta caacgtggct caatgcgatt tgttgtaggc 360 gacaaagtca atcaaggtga cattattggt ttacaaggta atagcaacta ttacgacaat 420 cctatgagtg tacatttaca tttacaatta cgccctaaag acgcaaagaa agatgaaaaa 480 tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540 caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600 aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660 tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720 acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780 ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840 tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900 gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960 aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020 ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080 ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140 actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200 aaagcaacag attggaaaca gaataaagat ggcatttggt ataaagctga acatgcttcg 1260 ttcacagtga cagcaccaga gggaattatc acaagataca aaggtccttg gactggtcac 1320 ccacaagctg gtgtattaca aaaaggtcaa acgattaaat atgatgaggt tcaaaaattt 1380 gacggtcatg tttgggtatc gtgggaaacg tttgagggcg aaactgtata catgccggta 1440 cgcacatggg acgctaaaac tggtaaagtt ggtaagttgt ggggcgaaat taaagagctc 1500 atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc 1560 acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat 1620 tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac 1680 gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt 1740 ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt 1800 ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa I860 ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct 1920 aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat 1980 gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg 2040 aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt 2100 caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg 2160 ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat 2220 agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa 2280 tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg 2340 gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag 2400 tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact 2460 tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact 2520 aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga 2580 aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa 2640 aagcaagaag caaaacaaat tgtgaaagca acagattgga aacagaataa agatggcatt 2700 tggtataaag ctgaacatgc ttcgttcaca gtgacagcac cagagggaat tatcacaaga 2760 tacaaaggtc cttggactgg tcacccacaa gctggtgtat tacaaaaagg tcaaacgatt 2820 aaatatgatg aggttcaaaa atttgacggt catgtttggg tatcgtggga aacgtttgag 2880 ggcgaaactg tatacatgcc ggtacgcaca tgggacgcta aaactggtaa agttggtaag 2940 ttgtggggcg aaattaaata a 2961 <210>5 <211> 1983 <212> DNA <213> artificial <220> <223> CHAP11_M23-2638_Ami2638_CBD2638 <400>5 atgcaagcaa aattaactaa aaatgagttt atagagtggt tgaaaacttc tgagggaaaa 60 caattcaatg tggacttatg gtatggattt caatgctttg attatgccaa tgctggttgg 120 aaagttttgt ttggattact tctaaaaggt ttaggtgcaa aagatattcc gttcgctaac 180 aacttcgacg gattagctac tgtataccaa aatacaccgg acttcttagc acaacctggc 240 gacatggtgg tattcggtag caactacggt gctggatatg gtcacgttgc atgggtaatt 300 gaagcaactt tagattacat cattgtatat gagcagaatt ggctaggcgg tggctggact 360 gacggaatcg aacaacccgg ctggggttgg gaaaaagtta caagacgaca acatgcttat 420 gatttcccta tgtggtttat ccgtccgaat tttaaaagtg agacagcgcc acgatcagtt 480 caatctccta cacaagcacc taaaaaagaa acagctggat ccatgctaac tgctattgac 540 tatcttacga aaaaaggttg gaaaatatca tctgaccctc gcacttacga tggttaccct 600 aaaaactacg gctacagaaa ttaccatgaa aacggcatta attatgatga gttttgtggt 660 ggttatcata gagcttttga tgtttacagt aacgaaacta acgacgtgcc tgctgttact 720 agcggaacag ttattgaagc aaacgattac ggtaattttg gtggtacatt cgttattaga 780 gacgctaacg ataacgattg gatatatggg catctacaac gtggctcaat gcgatttgtt 840 gtaggcgaca aagtcaatca aggtgacatt attggtttac aaggtaatag caactattac 900 gacaatccta tgagtgtaca tttacattta caattacgcc ctaaagacgc aaagaaagat 960 gaaaaatcac aagtatgtag tggtttggct atggaaaaat atgacattac aaatttaaat 1020 gctaaacaag ataaatcaaa gaatgggagc gtgaaagagt tgaaacatat ctattcaaac 1080 catattaaag gtaacaagat tacagcacca aaacctagta ttcaaggtgt ggtcatccac 1140 aatgattatg gtagtatgac acctagtcaa tacttaccat ggttatatgc acgtgagaat 1200 aacggtacac acgttaacgg ttgggctagt gtttatgcaa atagaaacga agtgctttgg 1260 tatcatccga cagactacgt agagtggcat tgtggtaatc aatgggcaaa tgctaactta 1320 atcggatttg aagtgtgtga gtcgtatcct ggtagaatct cggacaaatt attcttagaa 1380 aatgaagaag cgacattgaa agtagctgcg gatgtgatga agtcgtacgg attaccagtt 1440 aatcgcaaca ctgtacgtct gcataacgaa ttcttcggaa cttcttgtcc acatcgttcg 1500 tgggacttgc atgttggcaa aggtgagcct tacacaacta ctaatattaa taaaatgaaa 1560 gactacttca tcaaacgcat caaacattat tatgacggtg gaaagctaga agtaagcaaa 1620 gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 1680 attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 1740 gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 1800 ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1860 aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1920 ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1980 taa 1983 <210> 6 <211> 2109 <212> DNA <213> artificial <220> <223> Ami11_M23-2638_Ami2638_CBD2638 <400>6 aagccacaac ctaaagcagt agaacttaaa atcatcaaag atgtggttaa aggttatgac 60 ctacctaagc gtggtagtaa ccctaaaggt atagttatac acaacgacgc agggagcaaa 120 ggggcgactg ctgaagcata tcgtaacgga ttagtaaatg cacctttatc aagattagaa 180 gcgggcattg cgcatagtta cgtatcaggc aacacagttt ggcaagcctt agatgaatca 240 caagtaggtt ggcataccgc taatcaaata ggtaataaat attattacgg tattgaagta 300 tgtcaatcaa tgggcgcaga taacgcgaca ttcttaaaaa atgaacaggc aactttccaa 360 gaatgcgcta gattgttgaa aaaatgggga ttaccagcaa acagaaatac aatcagattg 420 cacaatgaat ttacttcaac atcatgccct catagaagtt cggttttaca cactggtttt 480 gacccagtaa ctcgcggtct attgccagaa gacaagcggt tgcaacttaa agactacttt 540 atcaagcaga ttagggcgta catggatggt aaaataccgg ttgccactgt ctctaatgag 600 tcaagcgctt caagtaatac agttaaacca gttgcaagtg caggatccat gctaactgct 660 attgactatc ttacgaaaaa aggttggaaa atatcatctg accctcgcac ttacgatggt 720 taccctaaaa actacggcta cagaaattac catgaaaacg gcattaatta tgatgagttt 780 tgtggtggtt atcatagagc ttttgatgtt tacagtaacg aaactaacga cgtgcctgct 840 gttactagcg gaacagttat tgaagcaaac gattacggta attttggtgg tacattcgtt 900 attagagacg ctaacgataa cgattggata tatgggcatc tacaacgtgg ctcaatgcga 960 tttgttgtag gcgacaaagt caatcaaggt gacattattg gtttacaagg taatagcaac 1020 tattacgaca atcctatgag tgtacattta catttacaat tacgccctaa agacgcaaag 1080 aaagatgaaa aatcacaagt atgtagtggt ttggctatgg aaaaatatga cattacaaat 1140 ttaaatgcta aacaagataa atcaaagaat gggagcgtga aagagttgaa acatatctat 1200 tcaaaccata ttaaaggtaa caagattaca gcaccaaaac ctagtattca aggtgtggtc 1260 atccacaatg attatggtag tatgacacct agtcaatact taccatggtt atatgcacgt 1320 gagaataacg gtacacacgt taacggttgg gctagtgttt atgcaaatag aaacgaagtg 1380 ctttggtatc atccgacaga ctacgtagag tggcattgtg gtaatcaatg ggcaaatgct 1440 aacttaatcg gatttgaagt gtgtgagtcg tatcctggta gaatctcgga caaattattc 1500 ttagaaaatg aagaagcgac attgaaagta gctgcggatg tgatgaagtc gtacggatta 1560 ccagttaatc gcaacactgt acgtctgcat aacgaattct tcggaacttc ttgtccacat 1620 cgttcgtggg acttgcatgt tggcaaaggt gagccttaca caactactaa tattaataaa 1680 atgaaagact acttcatcaa acgcatcaaa cattattatg acggtggaaa gctagaagta 1740 agcaaagcag caactatcaa acaatctgac gttaagcaag aagttaaaaa gcaagaagca 1800 aaacaaattg tgaaagcaac agattggaaa cagaataaag atggcatttg gtataaagct 1860 gaacatgctt cgttcacagt gacagcacca gagggaatta tcacaagata caaaggtcct 1920 tggactggtc acccacaagc tggtgtatta caaaaaggtc aaacgattaa atatgatgag 1980 gttcaaaaat ttgacggtca tgtttgggta tcgtgggaaa cgtttgaggg cgaaactgta 2040 tacatgccgg tacgcacatg ggacgctaaa actggtaaag ttggtaagtt gtggggcgaa 2100 attaaataa 2109 <210>7 <211> 1923 <212> DNA <213> artificial <220> <223> CHAPTw_Ami2638_M23-LST_CBD2638 <400>7 atgaaaaccc tgaaacaagc agagtcctac attaagagta aagtaaatac aggaactgat 60 tttgatggtt tatatgggta tcagtgtatg gacttagcag tagattatat ttaccatgta 120 acagatggta aaataagaat gtggggtaat gctaaggatg cgataaataa ctcttttggt 180 ggtactgcta cggtatataa aaactaccct gcttttagac ctaagtacgg tgatgtagtc 240 gtatggacta ctggtaattt tgcaacttat ggtcatatcg caatagttac taaccctgac 300 ccttatggag accttcaata tgttacagtt cttgaacaaa actggaacgg taacgggatt 360 tataaaaccg agttagctac aatcagaaca cacgattaca caggaattac acattttatt 420 aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat 480 gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg 540 aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt 600 caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg 660 ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat 720 agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa 780 tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg 840 gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag 900 tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact 960 tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact 1020 aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga 1080 aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa 1140 aagcaagaag caaaacaaat tgtgaaagca acagatgctg caacacatga acattcagca 1200 caatggttga ataattacaa aaaaggatat ggttacggtc cttatccatt aggtataaat 1260 ggcggtatgc actacggagt tgattttttt atgaatattg gaacaccagt aaaagctatt 1320 tcaagcggaa aaatagttga agctggttgg agtaattacg gaggaggtaa tcaaataggt 1380 cttattgaaa atgatggagt gcatagacaa tggtatatgc atctaagtaa atataatgtt 1440 aaagtaggag attatgtcaa agctggtcaa ataatcggtt ggtctggaag cactggttat 1500 tctacagcac cacatttaca cttccaaaga atggttaatt cattttcaaa ttcaactgcc 1560 caagatccaa tgcctttctt aaagagcgca ggatatggaa aagcaggtgg tacagtaact 1620 ccaacgccga atacaggttg gaaacagaat aaagatggca tttggtataa agctgaacat 1680 gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 1740 ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1800 aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1860 ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1920 taa 1923 <210> 8 <211 > 1953 <212> DNA <213> artificial <220> <223> His-tagged CHAPTw_Ami2638_M23-LST_CWT-LST <400>8 atgagaggat cgcatcacca tcaccatcac ggatccatga aaaccctgaa acaagcagag 60 tcctacatta agagtaaagt aaatacagga actgattttg atggtttata tgggtatcag 120 tgtatggact tagcagtaga ttatatttac catgtaacag atggtaaaat aagaatgtgg 180 ggtaatgcta aggatgcgat aaataactct tttggtggta ctgctacggt atataaaaac 240 taccctgctt ttagacctaa gtacggtgat gtagtcgtat ggactactgg taattttgca 300 acttatggtc atatcgcaat agttactaac cctgaccctt atggagacct tcaatatgtt 360 acagttcttg aacaaaactg gaacggtaac gggatttata aaaccgagtt agctacaatc 420 agaacacacg attacacagg aattacacat tttattaaag acgcaaagaa agatgaaaaa 480 tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540 caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600 aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660 tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720 acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780 ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840 tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900 gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960 aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020 ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080 ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140 actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200 aaagcaacag atgctgcaac acatgaacat tcagcacaat ggttgaataa ttacaaaaaa 1260 ggatatggtt acggtcctta tccattaggt ataaatggcg gtatgcacta cggagttgat 1320 ttttttatga atattggaac accagtaaaa gctatttcaa gcggaaaaat agttgaagct 1380 ggttggagta attacggagg aggtaatcaa ataggtctta ttgaaaatga tggagtgcat 1440 agacaatggt atatgcatct aagtaaatat aatgttaaag taggagatta tgtcaaagct 1500 ggtcaaataa tcggttggtc tggaagcact ggttattcta cagcaccaca tttacacttc 1560 caaagaatgg ttaattcatt ttcaaattca actgcccaag atccaatgcc tttcttaaag 1620 agcgcaggat atggaaaagc aggtggtaca gtaactccaa cgccgaatac aggttggaaa 1680 acaaacaaat atggcacact atataaatca gagtcagcta gcttcacacc taatacagat 1740 ataataacaa gaacgactgg tccatttaga agcatgccgc agtcaggagt cttaaaagca 1800 ggtcaaacaa ttcattatga tgaagtgatg aaacaagacg gtcatgtttg ggtaggttat 1860 acaggtaaca gtggccaacg tatttacttg cctgtaagaa catggaataa atctactaat 1920 actttaggtg ttctttgggg aactataaag taa 1953 <210>9 <211 > 1518 <212> DNA <213> artificial <220> <223> M23-LST_Ami2638_CBD2638 <400>9 gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac 60 ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat 120 attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat 180 tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat 240 atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc 300 ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt 360 aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat 420 ggaaaagcag gtggtacagt aactccaacg ccgaatacag gtgagctctt acgccctaaa 480 gacgcaaaga aagatgaaaa atcacaagta tgtagtggtt tggctatgga aaaatatgac 540 attacaaatt taaatgctaa acaagataaa tcaaagaatg ggagcgtgaa agagttgaaa 600 catatctatt caaaccatat taaaggtaac aagattacag caccaaaacc tagtattcaa 660 ggtgtggtca tccacaatga ttatggtagt atgacaccta gtcaatactt accatggtta 720 tatgcacgtg agaataacgg tacacacgtt aacggttggg ctagtgttta tgcaaataga 780 aacgaagtgc tttggtatca tccgacagac tacgtagagt ggcattgtgg taatcaatgg 840 gcaaatgcta acttaatcgg atttgaagtg tgtgagtcgt atcctggtag aatctcggac 900 aaattattct tagaaaatga agaagcgaca ttgaaagtag ctgcggatgt gatgaagtcg 960 tacggattac cagttaatcg caacactgta cgtctgcata acgaattctt cggaacttct 1020 tgtccacatc gttcgtggga cttgcatgtt ggcaaaggtg agccttacac aactactaat 1080 attaataaaa tgaaagacta cttcatcaaa cgcatcaaac attattatga cggtggaaag 1140 ctagaagtaa gcaaagcagc aactatcaaa caatctgacg ttaagcaaga agttaaaaag 1200 caagaagcaa aacaaattgt gaaagcaaca gattggaaac agaataaaga tggcatttgg 1260 tataaagctg aacatgcttc gttcacagtg acagcaccag agggaattat cacaagatac 1320 aaaggtcctt ggactggtca cccacaagct ggtgtattac aaaaaggtca aacgattaaa 1380 tatgatgagg ttcaaaaatt tgacggtcat gtttgggtat cgtgggaaac gtttgagggc 1440 gaaactgtat acatgccggt acgcacatgg gacgctaaaa ctggtaaagt tggtaagttg 1500 tggggcgaaa ttaaataa 1518 <210> 10 <211 > 1521 <212> DNA <213> artificial <220> <223> His-tagged LST_LST <400> 10 atgagaggat cgcatcacca tcaccatcac ggatccgctg caacacatga acattcagca 60 caatggttga ataattacaa aaaaggatat ggttacggtc cttatccatt aggtataaat 120 ggcggtatgc actacggagt tgattttttt atgaatattg gaacaccagt aaaagctatt 180 tcaagcggaa aaatagttga agctggttgg agtaattacg gaggaggtaa tcaaataggt 240 cttattgaaa atgatggagt gcatagacaa tggtatatgc atctaagtaa atataatgtt 300 aaagtaggag attatgtcaa agctggtcaa ataatcggtt ggtctggaag cactggttat 360 tctacagcac cacatttaca cttccaaaga atggttaatt cattttcaaa ttcaactgcc 420 caagatccaa tgcctttctt aaagagcgca ggatatggaa aagcaggtgg tacagtaact 480 ccaacgccga atacaggttg gaaaacaaac aaatatggca cactatataa atcagagtca 540 gctagcttca cacctaatac agatataata acaagaacga ctggtccatt tagaagcatg 600 ccgcagtcag gagtcttaaa agcaggtcaa acaattcatt atgatgaagt gatgaaacaa 660 gacggtcatg tttgggtagg ttatacaggt aacagtggcc aacgtattta cttgcctgta 720 agaacatgga ataaatctac taatacttta ggtgttcttt ggggaactat aaaggagctc 780 gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac 840 ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat 900 attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat 960 tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat 1020 atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc 1080 ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt 1140 aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat 1200 ggaaaagcag gtggtacagt aactccaacg ccgaatacag gttggaaaac aaacaaatat 1260 ggcacactat ataaatcaga gtcagctagc ttcacaccta atacagatat aataacaaga 1320 acgactggtc catttagaag catgccgcag tcaggagtct taaaagcagg tcaaacaatt 1380 cattatgatg aagtgatgaa acaagacggt catgtttggg taggttatac aggtaacagt 1440 ggccaacgta tttacttgcc tgtaagaaca tggaataaat ctactaatac tttaggtgtt 1500 ctttggggaa ctataaagtg a 1521 <210 11 <211> 1245 <212> DNA <213> artificial <220 <223> His-tagged M23-LST_M23_LST-CWT-LST <400 11 atgagaggat cgcatcacca tcaccatcac ggatccgctg caacacatga acattcagca 60 caatggttga ataattacaa aaaaggatat ggttacggtc cttatccatt aggtataaat 120 ggcggtatgc actacggagt tgattttttt atgaatattg gaacaccagt aaaagctatt 180 tcaagcggaa aaatagttga agctggttgg agtaattacg gaggaggtaa tcaaataggt 240 cttattgaaa atgatggagt gcatagacaa tggtatatgc atctaagtaa atataatgtt 300 aaagtaggag attatgtcaa agctggtcaa ataatcggtt ggtctggaag cactggttat 360 tctacagcac cacatttaca cttccaaaga atggttaatt cattttcaaa ttcaactgcc 420 caagatccaa tgcctttctt aaagagcgca ggatatggaa aagcaggtgg tacagtaact 480 ccaacgccga atacaggtga gctcgctgca acacatgaac attcagcaca atggttgaat 540 aattacaaaa aaggatatgg ttacggtcct tatccattag gtataaatgg cggtatgcac 600 tacggagttg atttttttat gaatattgga acaccagtaa aagctatttc aagcggaaaa 660 atagttgaag ctggttggag taattacgga ggaggtaatc aaataggtct tattgaaaat 720 gatggagtgc atagacaatg gtatatgcat ctaagtaaat ataatgttaa agtaggagat 780 tatgtcaaag ctggtcaaat aatcggttgg tctggaagca ctggttattc tacagcacca 840 catttacact tccaaagaat ggttaattca ttttcaaatt caactgccca agatccaatg 900 cctttcttaa agagcgcagg atatggaaaa gcaggtggta cagtaactcc aacgccgaat 960 acaggttgga aaacaaacaa atatggcaca ctatataaat cagagtcagc tagcttcaca 1020 cctaatacag atataataac aagaacgact ggtccattta gaagcatgcc gcagtcagga 1080 gtcttaaaag caggtcaaac aattcattat gatgaagtga tgaaacaaga cggtcatgtt 1140 tgggtaggtt atacaggtaa cagtggccaa cgtatttact tgcctgtaag aacatggaat 1200 aaatctacta atactttagg tgttctttgg ggaactataa agtga 1245

<210> 12 <211 > 285 <212> DNA <213> Staphylococcus aureus bacteriophage 2638a <400> 12 tggaaacaga ataaagatgg catttggtat aaagctgaac atgcttcgtt cacagtgaca 60 gcaccagagg gaattatcac aagatacaaa ggtccttgga ctggtcaccc acaagctggt 120 gtattacaaa aaggtcaaac gattaaatat gatgaggttc aaaaatttga cggtcatgtt 180 tgggtatcgt gggaaacgtt tgagggcgaa actgtataca tgccggtacg cacatgggac 240 gctaaaactg gtaaagttgg taagttgtgg ggcgaaatta aataa 285

<210> 13 <211 > 279 <212> DNA <213> Staphylococcus simulans <400> 13 tggaaaacaa acaaatatgg cacactatat aaatcagagt cagctagctt cacacctaat 60 acagatataa taacaagaac gactggtcca tttagaagca tgccgcagtc aggagtctta 120 aaagcaggtc aaacaattca ttatgatgaa gtgatgaaac aagacggtca tgtttgggta 180 ggttatacag gtaacagtgg ccaacgtatt tacttgcctg taagaacatg gaataaatct 240 actaatactt taggtgttct ttggggaact ataaagtga 279

<210> 14 <211 > 438 <212> DNA <213> Staphylococcus aureus bacteriophage 2638a <400> 14 atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc 60 acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat 120 tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac 180 gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt 240 ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt 300 ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa 360 ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct 420 aaagacgcaa agaaagat 438

<210> 15 <211 > 420 <212> DNA <213> Staphylococcus simulans <400> 15 gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac 60 ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat 120 attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat 180 tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat 240 atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc 300 ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt 360 aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat 420

<210> 16 <211 > 510 <212> DNA <213> Staphylococcus aureus bacteriophage 2638a <400> 16 ggtaacaaga ttacagcacc aaaacctagt attcaaggtg tggtcatcca caatgattat 60 ggtagtatga cacctagtca atacttacca tggttatatg cacgtgagaa taacggtaca 120 cacgttaacg gttgggctag tgtttatgca aatagaaacg aagtgctttg gtatcatccg 180 acagactacg tagagtggca ttgtggtaat caatgggcaa atgctaactt aatcggattt 240 gaagtgtgtg agtcgtatcc tggtagaatc tcggacaaat tattcttaga aaatgaagaa 300 gcgacattga aagtagctgc ggatgtgatg aagtcgtacg gattaccagt taatcgcaac 360 actgtacgtc tgcataacga attcttcgga acttcttgtc cacatcgttc gtgggacttg 420 catgttggca aaggtgagcc ttacacaact actaatatta ataaaatgaa agactacttc 480 atcaaacgca tcaaacatta ttatgacggt 510

<210> 17 <211 > 642 <212> DNA <213> Staphylococcus aureus phage phi 11 <400> 17 aagccacaac ctaaagcagt agaacttaaa atcatcaaag atgtggttaa aggttatgac 60 ctacctaagc gtggtagtaa ccctaaaggt atagttatac acaacgacgc agggagcaaa 120 ggggcgactg ctgaagcata tcgtaacgga ttagtaaatg cacctttatc aagattagaa 180 gcgggcattg cgcatagtta cgtatcaggc aacacagttt ggcaagcctt agatgaatca 240 caagtaggtt ggcataccgc taatcaaata ggtaataaat attattacgg tattgaagta 300 tgtcaatcaa tgggcgcaga taacgcgaca ttcttaaaaa atgaacaggc aactttccaa 360 gaatgcgcta gattgttgaa aaaatgggga ttaccagcaa acagaaatac aatcagattg 420 cacaatgaat ttacttcaac atcatgccct catagaagtt cggttttaca cactggtttt 480 gacccagtaa ctcgcggtct attgccagaa gacaagcggt tgcaacttaa agactacttt 540 atcaagcaga ttagggcgta catggatggt aaaataccgg ttgccactgt ctctaatgag 600 tcaagcgctt caagtaatac agttaaacca gttgcaagtg ca 642

<210> 18 <211> 516 <212> DNA <213> Staphylococcus aureus phage phi 11 <400> 18 atgcaagcaa aattaactaa aaatgagttt atagagtggt tgaaaacttc tgagggaaaa 60 caattcaatg tggacttatg gtatggattt caatgctttg attatgccaa tgctggttgg 120 aaagttttgt ttggattact tctaaaaggt ttaggtgcaa aagatattcc gttcgctaac 180 aacttcgacg gattagctac tgtataccaa aatacaccgg acttcttagc acaacctggc 240 gacatggtgg tattcggtag caactacggt gctggatatg gtcacgttgc atgggtaatt 300 gaagcaactt tagattacat cattgtatat gagcagaatt ggctaggcgg tggctggact 360 gacggaatcg aacaacccgg ctggggttgg gaaaaagtta caagacgaca acatgcttat 420 gatttcccta tgtggtttat ccgtccgaat tttaaaagtg agacagcgcc acgatcagtt 480 caatctccta cacaagcacc taaaaaagaa acagct 516

<210> 19 <211 > 420 <212> DNA <213> Staphylococcus phage Twort <400> 19 atgaaaaccc tgaaacaagc agagtcctac attaagagta aagtaaatac aggaactgat 60 tttgatggtt tatatgggta tcagtgtatg gacttagcag tagattatat ttaccatgta 120 acagatggta aaataagaat gtggggtaat gctaaggatg cgataaataa ctcttttggt 180 ggtactgcta cggtatataa aaactaccct gcttttagac ctaagtacgg tgatgtagtc 240 gtatggacta ctggtaattt tgcaacttat ggtcatatcg caatagttac taaccctgac 300 ccttatggag accttcaata tgttacagtt cttgaacaaa actggaacgg taacgggatt 360 tataaaaccg agttagctac aatcagaaca cacgattaca caggaattac acattttatt 420 <210> 20 <211 > 1851 <212> DNA <213> artificial <220> <223> M23-2638_Ami2638_CBD2638_CBD2638 <400> 20 atgctaactg ctattgacta tcttacgaaa aaaggttgga aaatatcatc tgaccctcgc 60 acttacgatg gttaccctaa aaactacggc tacagaaatt accatgaaaa cggcattaat 120 tatgatgagt tttgtggtgg ttatcataga gcttttgatg tttacagtaa cgaaactaac 180 gacgtgcctg ctgttactag cggaacagtt attgaagcaa acgattacgg taattttggt 240 ggtacattcg ttattagaga cgctaacgat aacgattgga tatatgggca tctacaacgt 300 ggctcaatgc gatttgttgt aggcgacaaa gtcaatcaag gtgacattat tggtttacaa 360 ggtaatagca actattacga caatcctatg agtgtacatt tacatttaca attacgccct 420 aaagacgcaa agaaagatga aaaatcacaa gtatgtagtg gtttggctat ggaaaaatat 480 gacattacaa atttaaatgc taaacaagat aaatcaaaga atgggagcgt gaaagagttg 540 aaacatatct attcaaacca tattaaaggt aacaagatta cagcaccaaa acctagtatt 600 caaggtgtgg tcatccacaa tgattatggt agtatgacac ctagtcaata cttaccatgg 660 ttatatgcac gtgagaataa cggtacacac gttaacggtt gggctagtgt ttatgcaaat 720 agaaacgaag tgctttggta tcatccgaca gactacgtag agtggcattg tggtaatcaa 780 tgggcaaatg ctaacttaat cggatttgaa gtgtgtgagt cgtatcctgg tagaatctcg 840 gacaaattat tcttagaaaa tgaagaagcg acattgaaag tagctgcgga tgtgatgaag 900 tcgtacggat taccagttaa tcgcaacact gtacgtctgc ataacgaatt cttcggaact 960 tcttgtccac atcgttcgtg ggacttgcat gttggcaaag gtgagcctta cacaactact 1020 aatattaata aaatgaaaga ctacttcatc aaacgcatca aacattatta tgacggtgga 1080 aagctagaag taagcaaagc agcaactatc aaacaatctg acgttaagca agaagttaaa 1140 aagcaagaag caaaacaaat tgtgaaagca acagattgga aacagaataa agatggcatt 1200 tggtataaag ctgaacatgc ttcgttcaca gtgacagcac cagagggaat tatcacaaga 1260 tacaaaggtc cttggactgg tcacccacaa gctggtgtat tacaaaaagg tcaaacgatt 1320 aaatatgatg aggttcaaaa atttgacggt catgtttggg tatcgtggga aacgtttgag 1380 ggcgaaactg tatacatgcc ggtacgcaca tgggacgcta aaactggtaa agttggtaag 1440 ttgtggggcg aaattaaaga gctcggtgga aagctagaag taagcaaagc agcaactatc 1500 aaacaatctg acgttaagca agaagttaaa aagcaagaag caaaacaaat tgtgaaagca 1560 acagattgga aacagaataa agatggcatt tggtataaag ctgaacatgc ttcgttcaca 1620 gtgacagcac cagagggaat tatcacaaga tacaaaggtc cttggactgg tcacccacaa 1680 gctggtgtat tacaaaaagg tcaaacgatt aaatatgatg aggttcaaaa atttgacggt 1740 catgtttggg tatcgtggga aacgtttgag ggcgaaactg tatacatgcc ggtacgcaca 1800 tgggacgcta aaactggtaa agttggtaag ttgtggggcg aaattaaata a 1851 <210>21 <211 >498 <212> PRT <213> artificial <220 <223> His-tagged Ply2638 <400 21

Met Arg Gly Ser His His His His His His Gly Ser Met Leu Thr Ala 15 10 15

Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg 20 25 30

Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu 35 40 45

Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe 50 55 60

Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly 65 70 75 80

Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val 85 90 95

Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gin Arg 100 105 110

Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn Gin Gly Asp Ile 115 120 125

Ile Gly Leu Gin Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val 130 135 140

His Leu His Leu Gin Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys 145 150 155 160

Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn 165 170 175

Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu 180 185 190

Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro 195 200 205

Lys Pro Ser Ile Gin Gly Val Val Ile His Asn Asp Tyr Gly Ser Met 210 215 220

Thr Pro Ser Gin Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly 225 230 235 240

Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val 245 250 255

Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gin 260 265 270

Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro 275 280 285

Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu 290 295 300

Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg 305 310 315 320

Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His 325 330 335

Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr 340 345 350

Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr 355 360 365

Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin 370 375 380

Ser Asp Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val 385 390 395 400

Lys Ala Thr Asp Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala 405 410 415

Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg 420 425 430

Tyr Lys Gly Pro Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys 435 440 445

Gly Gin Thr Ile Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val 450 455 460

Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val 465 470 475 480

Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu 485 490 495

Ile Lys <210> 22 <211 > 361 <212> PRT <213> artificial <220> <223> His-tagged Ami2638_CBD2638 <400> 22

Met Arg Gly Ser His His His His His His Gly Ser Leu Arg Pro Lys 15 10 15

Asp Ala Lys Lys Asp Glu Lys Ser Gin Val Cys Ser Gly Leu Ala Met 20 25 30

Glu Lys Tyr Asp Ile Thr Asn Leu Asn Ala Lys Gin Asp Lys Ser Lys 35 40 45

Asn Gly Ser Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys 50 55 60

Gly Asn Lys Ile Thr Ala Pro Lys Pro Ser Ile Gin Gly Val Val Ile 65 70 75 80

His Asn Asp Tyr Gly Ser Met Thr Pro Ser Gin Tyr Leu Pro Trp Leu 85 90 95

Tyr Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser Val 100 105 110

Tyr Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val 115 120 125

Glu Trp His Cys Gly Asn Gin Trp Ala Asn Ala Asn Leu Ile Gly Phe 130 135 140

Glu Val Cys Glu Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu 145 150 155 160

Glu Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser 165 170 175

Tyr Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe 180 185 190

Phe Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly Lys 195 200 205

Gly Glu Pro Tyr Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe 210 215 220

Ile Lys Arg Ile Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val Ser 225 230 235 240

Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys Gin Glu Val Lys Lys 245 250 255

Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp Trp Lys Gin Asn Lys 260 265 270

Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala 275 280 285

Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro 290 295 300

Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr Asp Glu Val 305 310 315 320

Gin Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu Gly 325 330 335

Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys 340 345 350

Val Gly Lys Leu Trp Gly Glu Ile Lys 355 360

<210> 23 <211 > 344 <212> PRT <213> artificial <220> <223> His-tagged M23-2638_CBD2638 <400> 23

Met Arg Gly Ser His His His His His His Gly Ser Met Leu Thr Ala 15 10 15

Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg 20 25 30

Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu 35 40 45

Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe 50 55 60

Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly 65 70 75 80

Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val 85 90 95

Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gin Arg 100 105 110

Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn Gin Gly Asp Ile 115 120 125

Ile Gly Leu Gin Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val 130 135 140

His Leu His Leu Gin Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys 145 150 155 160

Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn 165 170 175

Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu 180 185 190

Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro 195 200 205

Lys Pro Ser Ile Gin Gly Glu Leu Gly Gly Lys Leu Glu Val Ser Lys 210 215 220

Ala Ala Thr Ile Lys Gin Ser Asp Val Lys Gin Glu Val Lys Lys Gin 225 230 235 240

Glu Ala Lys Gin ile val Lys Ala Thr Asp Trp Lys Gin Asn Lys Asp 245 250 255

Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala Pro 260 265 270

Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro Gin 275 280 285

Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr Asp Glu Val Gin 290 295 300

Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu Gly Glu 305 310 315 320

Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys Val 325 330 335

Gly Lys Leu Trp Gly Glu Ile Lys 340 <210> 24 <211 > 986 <212> PRT <213> artificial <220> <223> His-tagged Ply2638_Ply2638 <400> 24

Met Arg Gly Ser His His His His His His Gly Ser Met Leu Thr Ala 15 10 15

Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg 20 25 30

Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu 35 40 45

Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe 50 55 60

Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly 65 70 75 80

Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val 85 90 95

Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gin Arg 100 105 110

Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn Gin Gly Asp Ile 115 120 125

Ile Gly Leu Gin Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val 130 135 140

His Leu His Leu Gin Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys 145 150 155 160

Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn 165 170 175

Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu 180 185 190

Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro 195 200 205

Lys Pro Ser Ile Gin Gly Val Val Ile His Asn Asp Tyr Gly Ser Met 210 215 220

Thr Pro Ser Gin Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly 225 230 235 240

Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val 245 250 255

Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gin 260 265 270

Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro 275 280 285

Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu 290 295 300

Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg 305 310 315 320

Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His 325 330 335

Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr 340 345 350

Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr 355 360 365

Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin 370 375 380

Ser Asp Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val 385 390 395 400

Lys Ala Thr Asp Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala 405 410 415

Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg 420 425 430

Tyr Lys Gly Pro Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys 435 440 445

Gly Gin Thr Ile Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val 450 455 460

Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val 465 470 475 480

Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu 485 490 495

Ile Lys Glu Leu Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly 500 505 510

Trp Lys Ile Ser Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn 515 520 525

Tyr Gly Tyr Arg Asn Tyr His Glu Asn Gly Ile Asn Tyr Asp Glu Phe 530 535 540

Cys Gly Gly Tyr His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn 545 550 555 560

Asp Val Pro Ala Val Thr Ser Gly Thr Val Ile Glu Ala Asn Asp Tyr 565 570 575

Gly Asn Phe Gly Gly Thr Phe Val Ile Arg Asp Ala Asn Asp Asn Asp 580 585 590

Trp Ile Tyr Gly His Leu Gin Arg Gly Ser Met Arg Phe Val Val Gly 595 600 605

Asp Lys Val Asn Gin Gly Asp Ile Ile Gly Leu Gin Gly Asn Ser Asn 610 615 620

Tyr Tyr Asp Asn Pro Met Ser Val His Leu His Leu Gin Leu Arg Pro 625 630 635 640

Lys Asp Ala Lys Lys Asp Glu Lys Ser Gin Val Cys Ser Gly Leu Ala 645 650 655

Met Glu Lys Tyr Asp Ile Thr Asn Leu Asn Ala Lys Gin Asp Lys Ser 660 665 670

Lys Asn Gly Ser Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile 675 680 685

Lys Gly Asn Lys Ile Thr Ala Pro Lys Pro Ser Ile Gin Gly Val Val 690 695 700

Ile His Asn Asp Tyr Gly Ser Met Thr Pro Ser Gin Tyr Leu Pro Trp 705 710 715 720

Leu Tyr Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser 725 730 735

Val Tyr Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr 740 745 750

Val Glu Trp His Cys Gly Asn Gin Trp Ala Asn Ala Asn Leu Ile Gly 755 760 765

Phe Glu Val Cys Glu Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe 770 775 780

Leu Glu Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys 785 790 795 800

Ser Tyr Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu 805 810 815

Phe Phe Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly 820 825 830

Lys Gly Glu Pro Tyr Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr 835 840 845

Phe Ile Lys Arg Ile Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val 850 855 860

Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys Gin Glu Val Lys 865 870 875 880

Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp Trp Lys Gin Asn 885 890 895

Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr 900 905 910

Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His 915 920 925

Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr Asp Glu 930 935 940

Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu 945 950 955 960

Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly 965 970 975

Lys Val Gly Lys Leu Trp Gly Glu Ile Lys 980 985 <210> 25 <211 > 672 <212> PRT <213> artificial <220> <223> His-tagged CHAP11_M23-2638_Ami2638_CBD2638 <400> 25

Met Arg Gly Ser His His His His His His Gly Ser Met Gin Ala Lys 15 10 15

Leu Thr Lys Asn Glu Phe Ile Glu Trp Leu Lys Thr Ser Glu Gly Lys 20 25 30

Gin Phe Asn Val Asp Leu Trp Tyr Gly Phe Gin Cys Phe Asp Tyr Ala 35 40 45

Asn Ala Gly Trp Lys Val Leu Phe Gly Leu Leu Leu Lys Gly Leu Gly 50 55 60

Ala Lys Asp Ile Pro Phe Ala Asn Asn Phe Asp Gly Leu Ala Thr Val 65 70 75 80

Tyr Gin Asn Thr Pro Asp Phe Leu Ala Gin Pro Gly Asp Met Val Val 85 90 95

Phe Gly Ser Asn Tyr Gly Ala Gly Tyr Gly His Val Ala Trp Val Ile 100 105 110

Glu Ala Thr Leu Asp Tyr Ile Ile Val Tyr Glu Gin Asn Trp Leu Gly 115 120 125

Gly Gly Trp Thr Asp Gly Ile Glu Gin Pro Gly Trp Gly Trp Glu Lys 130 135 140

Val Thr Arg Arg Gin His Ala Tyr Asp Phe Pro Met Trp Phe Ile Arg 145 150 155 160

Pro Asn Phe Lys Ser Glu Thr Ala Pro Arg Ser Val Gin Ser Pro Thr 165 170 175

Gin Ala Pro Lys Lys Glu Thr Ala Gly Ser Met Leu Thr Ala Ile Asp 180 185 190

Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg Thr Tyr 195 200 205

Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu Asn Gly 210 215 220

Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe Asp Val 225 230 235 240

Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly Thr Val 245 250 255

Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val Ile Arg 260 265 270

Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gin Arg Gly Ser 275 280 285

Met Arg Phe Val Val Gly Asp Lys Val Asn Gin Gly Asp Ile Ile Gly 290 295 300

Leu Gin Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val His Leu 305 310 315 320

His Leu Gin Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys Ser Gin 325 330 335

Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn Leu Asn 340 345 350

Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu Lys His 355 360 365

Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro Lys Pro 370 375 380

Ser Ile Gin Gly Val Val Ile His Asn Asp Tyr Gly Ser Met Thr Pro 385 390 395 400

Ser Gin Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly Thr His 405 410 415

Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val Leu Trp 420 425 430

Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gin Trp Ala 435 440 445

Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro Gly Arg 450 455 460

Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu Lys Val 465 470 475 480

Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg Asn Thr 485 490 495

Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His Arg Ser 500 505 510

Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr Asn Ile 515 520 525

Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr Tyr Asp 530 535 540

Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp 545 550 555 560

Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val Lys Ala 565 570 575

Thr Asp Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His 580 585 590

Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys 595 600 605

Gly Pro Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin 610 615 620

Thr Ile Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val 625 630 635 640

Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr 645 650 655

Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys 660 665 670 <210> 26 <211 > 714 <212> PRT <213> artificial <220> <223> His-tagged Ami11_M23-2638_Ami2638_CBD2638 <400> 26

Met Arg Gly Ser His His His His His His Gly Ser Lys Pro Gin Pro 15 10 15

Lys Ala Val Glu Leu Lys Ile Ile Lys Asp Val Val Lys Gly Tyr Asp 20 25 30

Leu Pro Lys Arg Gly Ser Asn Pro Lys Gly Ile Val Ile His Asn Asp 35 40 45

Ala Gly Ser Lys Gly Ala Thr Ala Glu Ala Tyr Arg Asn Gly Leu Val 50 55 60

Asn Ala Pro Leu Ser Arg Leu Glu Ala Gly Ile Ala His Ser Tyr Val 65 70 75 80

Ser Gly Asn Thr Val Trp Gin Ala Leu Asp Glu Ser Gin Val Gly Trp 85 90 95

His Thr Ala Asn Gin Ile Gly Asn Lys Tyr Tyr Tyr Gly Ile Glu Val 100 105 110

Cys Gin Ser Met Gly Ala Asp Asn Ala Thr Phe Leu Lys Asn Glu Gin 115 120 125

Ala Thr Phe Gin Glu Cys Ala Arg Leu Leu Lys Lys Trp Gly Leu Pro 130 135 140

Ala Asn Arg Asn Thr Ile Arg Leu His Asn Glu Phe Thr Ser Thr Ser 145 150 155 160

Cys Pro His Arg Ser Ser Val Leu His Thr Gly Phe Asp Pro Val Thr 165 170 175

Arg Gly Leu Leu Pro Glu Asp Lys Arg Leu Gin Leu Lys Asp Tyr Phe 180 185 190

Ile Lys Gin Ile Arg Ala Tyr Met Asp Gly Lys Ile Pro Val Ala Thr 195 200 205

Val Ser Asn Glu Ser Ser Ala Ser Ser Asn Thr Val Lys Pro Val Ala 210 215 220

Ser Ala Gly Ser Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly 225 230 235 240

Trp Lys Ile Ser Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn 245 250 255

Tyr Gly Tyr Arg Asn Tyr His Glu Asn Gly Ile Asn Tyr Asp Glu Phe 260 265 270

Cys Gly Gly Tyr His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn 275 280 285

Asp Val Pro Ala Val Thr Ser Gly Thr Val Ile Glu Ala Asn Asp Tyr 290 295 300

Gly Asn Phe Gly Gly Thr Phe Val Ile Arg Asp Ala Asn Asp Asn Asp 305 310 315 320

Trp Ile Tyr Gly His Leu Gin Arg Gly Ser Met Arg Phe Val Val Gly 325 330 335

Asp Lys Val Asn Gin Gly Asp Ile Ile Gly Leu Gin Gly Asn Ser Asn 340 345 350

Tyr Tyr Asp Asn Pro Met Ser Val His Leu His Leu Gin Leu Arg Pro 355 360 365

Lys Asp Ala Lys Lys Asp Glu Lys Ser Gin Val Cys Ser Gly Leu Ala 370 375 380

Met Glu Lys Tyr Asp Ile Thr Asn Leu Asn Ala Lys Gin Asp Lys Ser 385 390 395 400

Lys Asn Gly Ser Val Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile 405 410 415

Lys Gly Asn Lys Ile Thr Ala Pro Lys Pro Ser Ile Gin Gly Val Val 420 425 430

Ile His Asn Asp Tyr Gly Ser Met Thr Pro Ser Gin Tyr Leu Pro Trp 435 440 445

Leu Tyr Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser 450 455 460

Val Tyr Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr 465 470 475 480

Val Glu Trp His Cys Gly Asn Gin Trp Ala Asn Ala Asn Leu Ile Gly 485 490 495

Phe Glu Val Cys Glu Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe 500 505 510

Leu Glu Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys 515 520 525

Ser Tyr Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu 530 535 540

Phe Phe Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly 545 550 555 560

Lys Gly Glu Pro Tyr Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr 565 570 575

Phe Ile Lys Arg Ile Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val 580 585 590

Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys Gin Glu Val Lys 595 600 605

Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp Trp Lys Gin Asn 610 615 620

Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr val Thr 625 630 635 640

Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His 645 650 655

Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr Asp Glu 660 665 670

Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr Phe Glu 675 680 685

Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly 690 695 700

Lys Val Gly Lys Leu Trp Gly Glu Ile Lys 705 710 <210> 27 <211 > 652 <212> PRT <213> artificial <220> <223> His-tagged CHAPTw_Ami2638_M23-LST_CBD2638 <400> 27

Met Arg Gly Ser His His His His His His Gly Ser Met Lys Thr Leu 15 10 15

Lys Gin Ala Glu Ser Tyr Ile Lys Ser Lys Val Asn Thr Gly Thr Asp 20 25 30

Phe Asp Gly Leu Tyr Gly Tyr Gin Cys Met Asp Leu Ala Val Asp Tyr 35 40 45

Ile Tyr His Val Thr Asp Gly Lys Ile Arg Met Trp Gly Asn Ala Lys 50 55 60

Asp Ala Ile Asn Asn Ser Phe Gly Gly Thr Ala Thr Val Tyr Lys Asn 65 70 75 80

Tyr Pro Ala Phe Arg Pro Lys Tyr Gly Asp Val Val Val Trp Thr Thr 85 90 95

Gly Asn Phe Ala Thr Tyr Gly His Ile Ala Ile Val Thr Asn Pro Asp 100 105 110

Pro Tyr Gly Asp Leu Gin Tyr Val Thr Val Leu Glu Gin Asn Trp Asn 115 120 125

Gly Asn Gly Ile Tyr Lys Thr Glu Leu Ala Thr Ile Arg Thr His Asp 130 135 140

Tyr Thr Gly Ile Thr His Phe Ile Lys Asp Ala Lys Lys Asp Glu Lys 145 150 155 160

Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn 165 170 175

Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu 180 185 190

Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro 195 200 205

Lys Pro Ser Ile Gin Gly Val Val Ile His Asn Asp Tyr Gly Ser Met 210 215 220

Thr Pro Ser Gin Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly 225 230 235 240

Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val 245 250 255

Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gin 260 265 270

Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro 275 280 285

Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu 290 295 300

Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg 305 310 315 320

Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His 325 330 335

Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr 340 345 350

Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr 355 360 365

Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin 370 375 380

Ser Asp Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val 385 390 395 400

Lys Ala Thr Asp Ala Ala Thr His Glu His Ser Ala Gin Trp Leu Asn 405 410 415

Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn 420 425 430

Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro 435 440 445

Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn 450 455 460

Tyr Gly Gly Gly Asn Gin Ile Gly Leu Ile Glu Asn Asp Gly Val His 465 470 475 480

Arg Gin Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp 485 490 495

Tyr Val Lys Ala Gly Gin Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr 500 505 510

Ser Thr Ala Pro His Leu His Phe Gin Arg Met Val Asn Ser Phe Ser 515 520 525

Asn Ser Thr Ala Gin Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr 530 535 540

Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys 545 550 555 560

Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr 565 570 575

Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr 580 585 590

Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr 595 600 605

Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr 610 615 620

Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys 625 630 635 640

Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys 645 650 <210> 28 <211 > 650 <212> PRT <213> artificial <220> <223> His-tagged CHAPTw_Ami2638_M23-LST_CWT-LST <400> 28

Met Arg Gly Ser His His His His His His Gly Ser Met Lys Thr Leu 15 10 15

Lys Gin Ala Glu Ser Tyr Ile Lys Ser Lys Val Asn Thr Gly Thr Asp 20 25 30

Phe Asp Gly Leu Tyr Gly Tyr Gin Cys Met Asp Leu Ala Val Asp Tyr 35 40 45

Ile Tyr His Val Thr Asp Gly Lys Ile Arg Met Trp Gly Asn Ala Lys 50 55 60

Asp Ala Ile Asn Asn Ser Phe Gly Gly Thr Ala Thr Val Tyr Lys Asn 65 70 75 80

Tyr Pro Ala Phe Arg Pro Lys Tyr Gly Asp Val Val Val Trp Thr Thr 85 90 95

Gly Asn Phe Ala Thr Tyr Gly His Ile Ala Ile Val Thr Asn Pro Asp 100 105 110

Pro Tyr Gly Asp Leu Gin Tyr Val Thr Val Leu Glu Gin Asn Trp Asn 115 120 125

Gly Asn Gly Ile Tyr Lys Thr Glu Leu Ala Thr Ile Arg Thr His Asp 130 135 140

Tyr Thr Gly Ile Thr His Phe Ile Lys Asp Ala Lys Lys Asp Glu Lys 145 150 155 160

Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn 165 170 175

Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu 180 185 190

Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro 195 200 205

Lys Pro Ser Ile Gin Gly Val Val Ile His Asn Asp Tyr Gly Ser Met 210 215 220

Thr Pro Ser Gin Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly 225 230 235 240

Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val 245 250 255

Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gin 260 265 270

Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro 275 280 285

Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu 290 295 300

Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg 305 310 315 320

Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His 325 330 335

Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr 340 345 350

Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr 355 360 365

Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin 370 375 380

Ser Asp Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val 385 390 395 400

Lys Ala Thr Asp Ala Ala Thr His Glu His Ser Ala Gin Trp Leu Asn 405 410 415

Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn 420 425 430

Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro 435 440 445

Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn 450 455 460

Tyr Gly Gly Gly Asn Gin Ile Gly Leu Ile Glu Asn Asp Gly Val His 465 470 475 480

Arg Gin Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp 485 490 495

Tyr Val Lys Ala Gly Gin Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr 500 505 510

Ser Thr Ala Pro His Leu His Phe Gin Arg Met Val Asn Ser Phe Ser 515 520 525

Asn Ser Thr Ala Gin Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr 530 535 540

Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys 545 550 555 560

Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr 565 570 575

Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met 580 585 590

Pro Gin Ser Gly Val Leu Lys Ala Gly Gin Thr Ile His Tyr Asp Glu 595 600 605

Val Met Lys Gin Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser 610 615 620

Gly Gin Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn 625 630 635 640

Thr Leu Gly Val Leu Trp Gly Thr Ile Lys 645 650 <210> 29 <211> 517 <212> PRT <213> artificial <220> <223> His-tagged M23-LST_Ami2638_CBD2638 <400> 29

Met Arg Gly Ser His His His His His His Gly Ser Ala Ala Thr His 15 10 15

Glu His Ser Ala Gin Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr 20 25 30

Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val Asp 35 40 45

Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly Lys 50 55 €0

Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gin Ile Gly 65 70 75 80

Leu Ile Glu Asn Asp Gly Val His Arg Gin Trp Tyr Met His Leu Ser 85 90 95

Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala Gly Gin Ile Ile 100 105 110

Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His Phe 115 120 125

Gin Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala Gin Asp Pro Met 130 135 140

Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val Thr 145 150 155 160

Pro Thr Pro Asn Thr Gly Glu Leu Leu Arg Pro Lys Asp Ala Lys Lys 165 170 175

Asp Glu Lys Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp 180 185 190

Ile Thr Asn Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val 195 200 205

Lys Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile 210 215 220

Thr Ala Pro Lys Pro Ser Ile Gin Gly Val Val Ile His Asn Asp Tyr 225 230 235 240

Gly Ser Met Thr Pro Ser Gin Tyr Leu Pro Trp Leu Tyr Ala Arg Glu 245 250 255

Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg 260 265 270

Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys 275 280 285

Gly Asn Gin Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu 290 295 300

Ser Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu 305 310 315 320

Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro 325 330 335

Val Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser 340 345 350

Cys Pro His Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr 355 360 365

Thr Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile 370 375 380

Lys His Tyr Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr 385 390 395 400

Ile Lys Gin Ser Asp Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys 405 410 415

Gin Ile Val Lys Ala Thr Asp Trp Lys Gin Asn Lys Asp Gly Ile Trp 420 425 430

Tyr Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile 435 440 445

Ile Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro Gin Ala Gly Val 450 455 460

Leu Gin Lys Gly Gin Thr Ile Lys Tyr Asp Glu Val Gin Lys Phe Asp 465 470 475 480

Gly His Val Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr 485 490 495

Met Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu 500 505 510

Trp Gly Glu Ile Lys 515 <210> 30 <211> 506 <212> PRT <213> artificial <220> <223> His-tagged LST_LST <400> 30

Met Arg Gly Ser His His His His His His Gly Ser Ala Ala Thr His 15 10 15

Glu His Ser Ala Gin Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr 20 25 30

Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val Asp 35 40 45

Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly Lys 50 55 60

Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gin Ile Gly 65 70 75 80

Leu Ile Glu Asn Asp Gly Val His Arg Gin Trp Tyr Met His Leu Ser 85 90 95

Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala Gly Gin Ile Ile 100 105 110

Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His Phe 115 120 125

Gin Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala Gin Asp Pro Met 130 135 140

Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val Thr 145 150 155 160

Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn Lys Tyr Gly Thr Leu Tyr 165 170 175

Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn Thr Asp Ile Ile Thr Arg 180 185 190

Thr Thr Gly Pro Phe Arg Ser Met Pro Gin Ser Gly Val Leu Lys Ala 195 200 205

Gly Gin Thr Ile His Tyr Asp Glu Val Met Lys Gin Asp Gly His Val 210 215 220

Trp Val Gly Tyr Thr Gly Asn Ser Gly Gin Arg Ile Tyr Leu Pro Val 225 230 235 240

Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu Gly Val Leu Trp Gly Thr 245 250 255

Ile Lys Glu Leu Ala Ala Thr His Glu His Ser Ala Gin Trp Leu Asn 260 265 270

Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly Ile Asn 275 280 285

Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn Ile Gly Thr Pro 290 295 300

Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala Gly Trp Ser Asn 305 310 315 320

Tyr Gly Gly Gly Asn Gin Ile Gly Leu Ile Glu Asn Asp Gly Val His 325 330 335

Arg Gin Trp Tyr Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp 340 345 350

Tyr Val Lys Ala Gly Gin Ile Ile Gly Trp Ser Gly Ser Thr Gly Tyr 355 360 365

Ser Thr Ala Pro His Leu His Phe Gin Arg Met Val Asn Ser Phe Ser 370 375 380

Asn Ser Thr Ala Gin Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr 385 390 395 400

Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys 405 410 415

Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr 420 425 430

Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met 435 440 445

Pro Gin Ser Gly Val Leu Lys Ala Gly Gin Thr Ile His Tyr Asp Glu 450 455 460

Val Met Lys Gin Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser 465 470 475 480

Gly Gin Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn 485 490 495

Thr Leu Gly Val Leu Trp Gly Thr Ile Lys 500 505 <210>31 <211 > 414 <212> PRT <213> artificial <220 <223> His-tagged M23-LST_M23-LST_CWT-LST <400 31

Met Arg Gly Ser His His His His His His Gly Ser Ala Ala Thr His 15 10 15

Glu His Ser Ala Gin Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr 20 25 30

Gly Pro Tyr Pro Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val Asp 35 40 45

Phe Phe Met Asn Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly Lys 50 55 60

Ile Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gin Ile Gly 65 70 75 80

Leu Ile Glu Asn Asp Gly Val His Arg Gin Trp Tyr Met His Leu Ser 85 90 95

Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala Gly Gin Ile Ile 100 105 110

Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His Phe 115 120 125

Gin Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala Gin Asp Pro Met 130 135 140

Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val Thr 145 150 155 160

Pro Thr Pro Asn Thr Gly Glu Leu Ala Ala Thr His Glu His Ser Ala 165 170 175

Gin Trp Leu Asn Asn Tyr Lys Lys Gly Tyr Gly Tyr Gly Pro Tyr Pro 180 185 190

Leu Gly Ile Asn Gly Gly Met His Tyr Gly Val Asp Phe Phe Met Asn 195 200 205

Ile Gly Thr Pro Val Lys Ala Ile Ser Ser Gly Lys Ile Val Glu Ala 210 215 220

Gly Trp Ser Asn Tyr Gly Gly Gly Asn Gin Ile Gly Leu Ile Glu Asn 225 230 235 240

Asp Gly Val His Arg Gin Trp Tyr Met His Leu Ser Lys Tyr Asn Val 245 250 255

Lys val Gly Asp Tyr val Lys Ala Gly Gin ile ile Gly Trp Ser Gly 260 265 270

Ser Thr Gly Tyr Ser Thr Ala Pro His Leu His Phe Gin Arg Met Val 275 280 285

Asn Ser Phe Ser Asn Ser Thr Ala Gin Asp Pro Met Pro Phe Leu Lys 290 295 300

Ser Ala Gly Tyr Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn 305 310 315 320

Thr Gly Trp Lys Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser 325 330 335

Ala Ser Phe Thr Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro 340 345 350

Phe Arg Ser Met Pro Gin Ser Gly Val Leu Lys Ala Gly Gin Thr Ile 355 360 365

His Tyr Asp Glu Val Met Lys Gin Asp Gly His Val Trp Val Gly Tyr 370 375 380

Thr Gly Asn Ser Gly Gin Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn 385 390 395 400

Lys Ser Thr Asn Thr Leu Gly Val Leu Trp Gly Thr Ile Lys 405 410 <210> 32 <211 > 628 <212> PRT <213> artificial <220> <223> His-tagged M23-2638_Ami2638_CBD2638_CBD2638 <400> 32

Met Arg Gly Ser His His His His His His Gly Ser Met Leu Thr Ala 15 10 15

Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser Ser Asp Pro Arg 20 25 30

Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg Asn Tyr His Glu 35 40 45

Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr His Arg Ala Phe 50 55 60

Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala Val Thr Ser Gly 65 70 75 80

Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly Gly Thr Phe Val 85 90 95

Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly His Leu Gin Arg 100 105 110

Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn Gin Gly Asp Ile 115 120 125

Ile Gly Leu Gin Gly Asn Ser Asn Tyr Tyr Asp Asn Pro Met Ser Val 130 135 140

His Leu His Leu Gin Leu Arg Pro Lys Asp Ala Lys Lys Asp Glu Lys 145 150 155 160

Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile Thr Asn 165 170 175

Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val Lys Glu Leu 180 185 190

Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr Ala Pro 195 200 205

Lys Pro Ser Ile Gin Gly Val Val Ile His Asn Asp Tyr Gly Ser Met 210 215 220

Thr Pro Ser Gin Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn Asn Gly 225 230 235 240

Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn Glu Val 245 250 255

Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly Asn Gin 260 265 270

Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser Tyr Pro 275 280 285

Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala Thr Leu 290 295 300

Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val Asn Arg 305 310 315 320

Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys Pro His 325 330 335

Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr Thr Thr 340 345 350

Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys His Tyr 355 360 365

Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin 370 375 380

Ser Asp Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val 385 390 395 400

Lys Ala Thr Asp Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala 405 410 415

Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg 420 425 430

Tyr Lys Gly Pro Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys 435 440 445

Gly Gin Thr Ile Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val 450 455 460

Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val 465 470 475 480

Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu 485 490 495

Ile Lys Glu Leu Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile 500 505 510

Lys Gin Ser Asp Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin 515 520 525

Ile Val Lys Ala Thr Asp Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr 530 535 540

Lys Ala Glu His Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile 545 550 555 560

Thr Arg Tyr Lys Gly Pro Trp Thr Gly His Pro Gin Ala Gly Val Leu 565 570 575

Gin Lys Gly Gin Thr Ile Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly 580 585 590

His Val Trp Val Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met 595 600 605

Pro Val Arg Thr Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp 610 615 620

Gly Glu Ile Lys 625

<210> 33 <211> 741 <212> DNA <213> Staphylococcus simulans <400> 33 gctgcaacac atgaacattc agcacaatgg ttgaataatt acaaaaaagg atatggttac 60 ggtccttatc cattaggtat aaatggcggt atgcactacg gagttgattt ttttatgaat 120 attggaacac cagtaaaagc tatttcaagc ggaaaaatag ttgaagctgg ttggagtaat 180 tacggaggag gtaatcaaat aggtcttatt gaaaatgatg gagtgcatag acaatggtat 240 atgcatctaa gtaaatataa tgttaaagta ggagattatg tcaaagctgg tcaaataatc 300 ggttggtctg gaagcactgg ttattctaca gcaccacatt tacacttcca aagaatggtt 360 aattcatttt caaattcaac tgcccaagat ccaatgcctt tcttaaagag cgcaggatat 420 ggaaaagcag gtggtacagt aactccaacg ccgaatacag gttggaaaac aaacaaatat 480 ggcacactat ataaatcaga gtcagctagc ttcacaccta atacagatat aataacaaga 540 acgactggtc catttagaag catgccgcag tcaggagtct taaaagcagg tcaaacaatt 600 cattatgatg aagtgatgaa acaagacggt catgtttggg taggttatac aggtaacagt 660 ggccaacgta tttacttgcc tgtaagaaca tggaataaat ctactaatac tttaggtgtt 720 ctttggggaa ctataaagtg a 741

<210> 34 <211> 246 <212> PRT <213> Staphylococcus simulans <400> 34

Ala Ala Thr His Glu His Ser Ala Gin Trp Leu Asn Asn Tyr Lys Lys 15 10 15

Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly lie Asn Gly Gly Met His 20 25 30

Tyr Gly Val Asp Phe Phe Met Asn lie Gly Thr Pro Val Lys Ala lie 35 40 45

Ser Ser Gly Lys lie Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 50 55 60

Asn Gin lie Gly Leu Ile Glu Asn Asp Gly Val His Arg Gin Trp Tyr 65 70 75 80

Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 85 90 95

Gly Gin lie lie Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 100 105 110

His Leu His Phe Gin Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 115 120 125

Gin Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 130 135 140

Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys Thr Asn Lys Tyr 145 150 155 160

Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr Pro Asn Thr Asp 165 170 175 lie He Thr Arg Thr Thr Gly Pro Phe Arg Ser Met Pro Gin Ser Gly 180 185 190

Val Leu Lys Ala Gly Gin Thr lie His Tyr Asp Glu Val Met Lys Gin 195 200 205

Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser Gly Gin Arg lie 210 215 220

Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn Thr Leu Gly Val 225 230 235 240

Leu Trp Gly Thr He Lys 245

<210> 35 <211 > 94 <212> PRT <213> Staphylococcus aureus bacteriophage 2638a <400> 35

Trp Lys Gin Asn Lys Asp Gly lie Trp Tyr Lys Ala Glu His Ala Ser 15 10 15

Phe Thr Val Thr Ala Pro Glu Gly lie lie Thr Arg Tyr Lys Gly Pro 20 25 30

Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr lie 35 40 45

Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp 50 55 60

Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp 65 70 75 80

Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu lie Lys 85 90

<210> 36 <211> 92 <212> PRT <213> Staphylococcus simulans <400> 36

Trp Lys Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser 15 10 15

Phe Thr Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg 20 25 30

Ser Met Pro Gin Ser Gly Val Leu Lys Ala Gly Gin Thr Ile His Tyr 35 40 45

Asp Glu Val Met Lys Gin Asp Gly His Val Trp Val Gly Tyr Thr Gly 50 55 60

Asn Ser Gly Gin Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser 65 70 75 80

Thr Asn Thr Leu Gly Val Leu Trp Gly Thr Ile Lys 85 90

<210> 37 <211 > 146 <212> PRT <213> Staphylococcus aureus bacteriophage 2638a <400> 37

Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser 15 10 15

Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg 20 25 30

Asn Tyr His Glu Asn Gly Ile Asn Tyr Asp Glu Phe Cys Gly Gly Tyr 35 40 45

His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala 50 55 60

Val Thr Ser Gly Thr Val Ile Glu Ala Asn Asp Tyr Gly Asn Phe Gly 65 70 75 80

Gly Thr Phe Val Ile Arg Asp Ala Asn Asp Asn Asp Trp Ile Tyr Gly 85 90 95

His Leu Gin Arg Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn 100 105 110

Gin Gly Asp Ile Ile Gly Leu Gin Gly Asn Ser Asn Tyr Tyr Asp Asn 115 120 125

Pro Met Ser Val His Leu His Leu Gin Leu Arg Pro Lys Asp Ala Lys 130 135 140

Lys Asp 145

<210> 38 <211 > 140 <212> PRT <213> Staphylococcus simulans <400> 38

Ala Ala Thr His Glu His Ser Ala Gin Trp Leu Asn Asn Tyr Lys Lys 15 10 15

Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly lie Asn Gly Gly Met His 20 25 30

Tyr Gly Val Asp Phe Phe Met Asn lie Gly Thr Pro Val Lys Ala lie 35 40 45

Ser Ser Gly Lys lie Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 50 55 60

Asn Gin lie Gly Leu Ile Glu Asn Asp Gly Val His Arg Gin Trp Tyr 65 70 75 80

Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 85 90 95

Gly Gin lie lie Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 100 105 110

His Leu His Phe Gin Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 115 120 125

Gin Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr 130 135 140

<210> 39 <211 > 169 <212> PRT <213> Staphylococcus aureus bacteriophage 2638a <400> 39

Asn Lys lie Thr Ala Pro Lys Pro Ser lie Gin Gly Val Val lie His 15 10 15

Asn Asp Tyr Gly Ser Met Thr Pro Ser Gin Tyr Leu Pro Trp Leu Tyr 20 25 30

Ala Arg Glu Asn Asn Gly Thr His Val Asn Gly Trp Ala Ser Val Tyr 35 40 45

Ala Asn Arg Asn Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val Glu 50 55 60

Trp His Cys Gly Asn Gin Trp Ala Asn Ala Asn Leu lie Gly Phe Glu 65 70 75 80

Val Cys Glu Ser Tyr Pro Gly Arg lie Ser Asp Lys Leu Phe Leu Glu 85 90 95

Asn Glu Glu Ala Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser Tyr 100 105 110

Gly Leu Pro Val Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe Phe 115 120 125

Gly Thr Ser Cys Pro His Arg Ser Trp Asp Leu His Val Gly Lys Gly 130 135 140

Glu Pro Tyr Thr Thr Thr Asn lie Asn Lys Met Lys Asp Tyr Phe lie 145 150 155 160

Lys Arg lie Lys His Tyr Tyr Asp Gly 165

<210>40 <211> 163 <212> PRT <213> Staphylococcus aureus phage phi 11 <400> 40

Asn Pro Lys Gly Ile Val Ile His Asn Asp Ala Gly Ser Lys Gly Ala 15 10 15

Thr Ala Glu Ala Tyr Arg Asn Gly Leu Val Asn Ala Pro Leu Ser Arg 20 25 30

Leu Glu Ala Gly Ile Ala His Ser Tyr Val Ser Gly Asn Thr Val Trp 35 40 45

Gin Ala Leu Asp Glu Ser Gin Val Gly Trp His Thr Ala Asn Gin Ile 50 55 60

Gly Asn Lys Tyr Tyr Tyr Gly Ile Glu Val Cys Gin Ser Met Gly Ala 65 70 75 80

Asp Asn Ala Thr Phe Leu Lys Asn Glu Gin Ala Thr Phe Gin Glu Cys 85 90 95

Ala Arg Leu Leu Lys Lys Trp Gly Leu Pro Ala Asn Arg Asn Thr Ile 100 105 110

Arg Leu His Asn Glu Phe Thr Ser Thr Ser Cys Pro His Arg Ser Ser 115 120 125

Val Leu His Thr Gly Phe Asp Pro Val Thr Arg Gly Leu Leu Pro Glu 130 135 140

Asp Lys Arg Leu Gin Leu Lys Asp Tyr Phe Ile Lys Gin Ile Arg Ala 145 150 155 160

Tyr Met Asp

<210>41 <211 > 158 <212> PRT <213> Staphylococcus aureus phage phi 11 <400>41

Met Ser Ile Ile Met Glu Val Ala Thr Met Gin Ala Lys Leu Thr Lys 15 10 15

Asn Glu Phe Ile Glu Trp Leu Lys Thr Ser Glu Gly Lys Gin Phe Asn 20 25 30

Val Asp Leu Trp Tyr Gly Phe Gin Cys Phe Asp Tyr Ala Asn Ala Gly 35 40 45

Trp Lys Val Leu Phe Gly Leu Leu Leu Lys Gly Leu Gly Ala Lys Asp 50 55 60

Ile Pro Phe Ala Asn Asn Phe Asp Gly Leu Ala Thr Val Tyr Gin Asn 65 70 75 80

Thr Pro Asp Phe Leu Ala Gin Pro Gly Asp Met Val Val Phe Gly Ser 85 90 95

Asn Tyr Gly Ala Gly Tyr Gly His Val Ala Trp Val Ile Glu Ala Thr 100 105 110

Leu Asp Tyr Ile Ile Val Tyr Glu Gin Asn Trp Leu Gly Gly Gly Trp 115 120 125

Thr Asp Gly Ile Glu Gin Pro Gly Trp Gly Trp Glu Lys Val Thr Arg 130 135 140

Arg Gin His Ala Tyr Asp Phe Pro Met Trp Phe Ile Arg Pro 145 150 155 <210> 42 <211 > 140

<212> PRT <213> Staphylococcus phage Twort <400> 42

Met Lys Thr Leu Lys Gin Ala Glu Ser Tyr Ile Lys Ser Lys Val Asn 15 10 15

Thr Gly Thr Asp Phe Asp Gly Leu Tyr Gly Tyr Gin Cys Met Asp Leu 20 25 30

Ala Val Asp Tyr Ile Tyr His Val Thr Asp Gly Lys Ile Arg Met Trp 35 40 45

Gly Asn Ala Lys Asp Ala Ile Asn Asn Ser Phe Gly Gly Thr Ala Thr 50 55 60

Val Tyr Lys Asn Tyr Pro Ala Phe Arg Pro Lys Tyr Gly Asp Val Val 65 70 75 80

Val Trp Thr Thr Gly Asn Phe Ala Thr Tyr Gly His Ile Ala Ile Val 85 90 95

Thr Asn Pro Asp Pro Tyr Gly Asp Leu Gin Tyr Val Thr Val Leu Glu 100 105 110

Gin Asn Trp Asn Gly Asn Gly Ile Tyr Lys Thr Glu Leu Ala Thr Ile 115 120 125

Arg Thr His Asp Tyr Thr Gly Ile Thr His Phe Ile 130 135 140 <210> 43 <211 > 12 <212> PRT <213> artificial <220> <223> His-tag <400> 43

Met Acg Gly Ser His His His His His His Gly Ser 15 10 <210> 44 <211 > 1497 <212> DNA <213> artificial <220> <223> His-tagged Ply2638 <400> 44 atgagaggat cgcatcacca tcaccatcac ggatccatgc taactgctat tgactatctt 60 acgaaaaaag gttggaaaat atcatctgac cctcgcactt acgatggtta ccctaaaaac 120 tacggctaca gaaattacca tgaaaacggc attaattatg atgagttttg tggtggttat 180 catagagctt ttgatgttta cagtaacgaa actaacgacg tgcctgctgt tactagcgga 240 acagttattg aagcaaacga ttacggtaat tttggtggta cattcgttat tagagacgct 300 aacgataacg attggatata tgggcatcta caacgtggct caatgcgatt tgttgtaggc 360 gacaaagtca atcaaggtga cattattggt ttacaaggta atagcaacta ttacgacaat 420 cctatgagtg tacatttaca tttacaatta cgccctaaag acgcaaagaa agatgaaaaa 480 tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540 caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600 aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660 tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720 acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780 ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840 tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900 gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960 aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020 ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080 ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140 actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200 aaagcaacag attggaaaca gaataaagat ggcatttggt ataaagctga acatgcttcg 1260 ttcacagtga cagcaccaga gggaattatc acaagataca aaggtccttg gactggtcac 1320 ccacaagctg gtgtattaca aaaaggtcaa acgattaaat atgatgaggt tcaaaaattt 1380 gacggtcatg tttgggtatc gtgggaaacg tttgagggcg aaactgtata catgccggta 1440 cgcacatggg acgctaaaac tggtaaagtt ggtaagttgt ggggcgaaat taaataa 1497 <210> 45 <211> 2019 <212> DNA <213> artificial <220> <223> His-tagged CHAP11-M23-2638_Ami2638_CBD2638 <400> 45 atgagaggat cgcatcacca tcaccatcac ggatccatgc aagcaaaatt aactaaaaat 60 gagtttatag agtggttgaa aacttctgag ggaaaacaat tcaatgtgga cttatggtat 120 ggatttcaat gctttgatta tgccaatgct ggttggaaag ttttgtttgg attacttcta 180 aaaggtttag gtgcaaaaga tattccgttc gctaacaact tcgacggatt agctactgta 240 taccaaaata caccggactt cttagcacaa cctggcgaca tggtggtatt cggtagcaac 300 tacggtgctg gatatggtca cgttgcatgg gtaattgaag caactttaga ttacatcatt 360 gtatatgagc agaattggct aggcggtggc tggactgacg gaatcgaaca acccggctgg 420 ggttgggaaa aagttacaag acgacaacat gcttatgatt tccctatgtg gtttatccgt 480 ccgaatttta aaagtgagac agcgccacga tcagttcaat ctcctacaca agcacctaaa 540 aaagaaacag ctggatccat gctaactgct attgactatc ttacgaaaaa aggttggaaa 600 atatcatctg accctcgcac ttacgatggt taccctaaaa actacggcta cagaaattac 660 catgaaaacg gcattaatta tgatgagttt tgtggtggtt atcatagagc ttttgatgtt 720 tacagtaacg aaactaacga cgtgcctgct gttactagcg gaacagttat tgaagcaaac 780 gattacggta attttggtgg tacattcgtt attagagacg ctaacgataa cgattggata 840 tatgggcatc tacaacgtgg ctcaatgcga tttgttgtag gcgacaaagt caatcaaggt 900 gacattattg gtttacaagg taatagcaac tattacgaca atcctatgag tgtacattta 960 catttacaat tacgccctaa agacgcaaag aaagatgaaa aatcacaagt atgtagtggt 1020 ttggctatgg aaaaatatga cattacaaat ttaaatgcta aacaagataa atcaaagaat 1080 gggagcgtga aagagttgaa acatatctat tcaaaccata ttaaaggtaa caagattaca 1140 gcaccaaaac ctagtattca aggtgtggtc atccacaatg attatggtag tatgacacct 1200 agtcaatact taccatggtt atatgcacgt gagaataacg gtacacacgt taacggttgg 1260 gctagtgttt atgcaaatag aaacgaagtg ctttggtatc atccgacaga ctacgtagag 1320 tggcattgtg gtaatcaatg ggcaaatgct aacttaatcg gatttgaagt gtgtgagtcg 1380 tatcctggta gaatctcgga caaattattc ttagaaaatg aagaagcgac attgaaagta 1440 gctgcggatg tgatgaagtc gtacggatta ccagttaatc gcaacactgt acgtctgcat 1500 aacgaattct tcggaacttc ttgtccacat cgttcgtggg acttgcatgt tggcaaaggt 1560 gagccttaca caactactaa tattaataaa atgaaagact acttcatcaa acgcatcaaa 1620 cattattatg acggtggaaa gctagaagta agcaaagcag caactatcaa acaatctgac 1680 gttaagcaag aagttaaaaa gcaagaagca aaacaaattg tgaaagcaac agattggaaa 1740 cagaataaag atggcatttg gtataaagct gaacatgctt cgttcacagt gacagcacca 1800 gagggaatta tcacaagata caaaggtcct tggactggtc acccacaagc tggtgtatta 1860 caaaaaggtc aaacgattaa atatgatgag gttcaaaaat ttgacggtca tgtttgggta 1920 tcgtgggaaa cgtttgaggg cgaaactgta tacatgccgg tacgcacatg ggacgctaaa 1980 actggtaaag ttggtaagtt gtggggcgaa attaaataa 2019 <210> 46 <211> 2145 <212> DNA <213> artificial <220> <223> His-tagged Ami11_M23-2638_Ami2638_CBD2638 <400> 46 atgagaggat cgcatcacca tcaccatcac ggatccaagc cacaacctaa agcagtagaa 60 cttaaaatca tcaaagatgt ggttaaaggt tatgacctac ctaagcgtgg tagtaaccct 120 aaaggtatag ttatacacaa cgacgcaggg agcaaagggg cgactgctga agcatatcgt 180 aacggattag taaatgcacc tttatcaaga ttagaagcgg gcattgcgca tagttacgta 240 tcaggcaaca cagtttggca agccttagat gaatcacaag taggttggca taccgctaat 300 caaataggta ataaatatta ttacggtatt gaagtatgtc aatcaatggg cgcagataac 360 gcgacattct taaaaaatga acaggcaact ttccaagaat gcgctagatt gttgaaaaaa 420 tggggattac cagcaaacag aaatacaatc agattgcaca atgaatttac ttcaacatca 480 tgccctcata gaagttcggt tttacacact ggttttgacc cagtaactcg cggtctattg 540 ccagaagaca agcggttgca acttaaagac tactttatca agcagattag ggcgtacatg 600 gatggtaaaa taccggttgc cactgtctct aatgagtcaa gcgcttcaag taatacagtt 660 aaaccagttg caagtgcagg atccatgcta actgctattg actatcttac gaaaaaaggt 720 tggaaaatat catctgaccc tcgcacttac gatggttacc ctaaaaacta cggctacaga 780 aattaccatg aaaacggcat taattatgat gagttttgtg gtggttatca tagagctttt 840 gatgtttaca gtaacgaaac taacgacgtg cctgctgtta ctagcggaac agttattgaa 900 gcaaacgatt acggtaattt tggtggtaca ttcgttatta gagacgctaa cgataacgat 960 tggatatatg ggcatctaca acgtggctca atgcgatttg ttgtaggcga caaagtcaat 1020 caaggtgaca ttattggttt acaaggtaat agcaactatt acgacaatcc tatgagtgta 1080 catttacatt tacaattacg ccctaaagac gcaaagaaag atgaaaaatc acaagtatgt 1140 agtggtttgg ctatggaaaa atatgacatt acaaatttaa atgctaaaca agataaatca 1200 aagaatggga gcgtgaaaga gttgaaacat atctattcaa accatattaa aggtaacaag 1260 attacagcac caaaacctag tattcaaggt gtggtcatcc acaatgatta tggtagtatg 1320 acacctagtc aatacttacc atggttatat gcacgtgaga ataacggtac acacgttaac 1380 ggttgggcta gtgtttatgc aaatagaaac gaagtgcttt ggtatcatcc gacagactac 1440 gtagagtggc attgtggtaa tcaatgggca aatgctaact taatcggatt tgaagtgtgt 1500 gagtcgtatc ctggtagaat ctcggacaaa ttattcttag aaaatgaaga agcgacattg 1560 aaagtagctg cggatgtgat gaagtcgtac ggattaccag ttaatcgcaa cactgtacgt 1620 ctgcataacg aattcttcgg aacttcttgt ccacatcgtt cgtgggactt gcatgttggc 1680 aaaggtgagc cttacacaac tactaatatt aataaaatga aagactactt catcaaacgc 1740 atcaaacatt attatgacgg tggaaagcta gaagtaagca aagcagcaac tatcaaacaa 1800 tctgacgtta agcaagaagt taaaaagcaa gaagcaaaac aaattgtgaa agcaacagat 1860 tggaaacaga ataaagatgg catttggtat aaagctgaac atgcttcgtt cacagtgaca 1920 gcaccagagg gaattatcac aagatacaaa ggtccttgga ctggtcaccc acaagctggt 1980 gtattacaaa aaggtcaaac gattaaatat gatgaggttc aaaaatttga cggtcatgtt 2040 tgggtatcgt gggaaacgtt tgagggcgaa actgtataca tgccggtacg cacatgggac 2100 gctaaaactg gtaaagttgg taagttgtgg ggcgaaatta aataa 2145 <210> 47 <211 > 1959 <212> DNA <213> artificial <220> <223> His-tagged CHAPTw_Ami2638_M23-LST_CBD2638 <400> 47 atgagaggat cgcatcacca tcaccatcac ggatccatga aaaccctgaa acaagcagag 60 tcctacatta agagtaaagt aaatacagga actgattttg atggtttata tgggtatcag 120 tgtatggact tagcagtaga ttatatttac catgtaacag atggtaaaat aagaatgtgg 180 ggtaatgcta aggatgcgat aaataactct tttggtggta ctgctacggt atataaaaac 240 taccctgctt ttagacctaa gtacggtgat gtagtcgtat ggactactgg taattttgca 300 acttatggtc atatcgcaat agttactaac cctgaccctt atggagacct tcaatatgtt 360 acagttcttg aacaaaactg gaacggtaac gggatttata aaaccgagtt agctacaatc 420 agaacacacg attacacagg aattacacat tttattaaag acgcaaagaa agatgaaaaa 480 tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540 caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600 aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660 tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720 acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780 ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840 tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900 gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960 aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020 ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080 ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140 actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200 aaagcaacag atgctgcaac acatgaacat tcagcacaat ggttgaataa ttacaaaaaa 1260 ggatatggtt acggtcctta tccattaggt ataaatggcg gtatgcacta cggagttgat 1320 ttttttatga atattggaac accagtaaaa gctatttcaa gcggaaaaat agttgaagct 1380 ggttggagta attacggagg aggtaatcaa ataggtctta ttgaaaatga tggagtgcat 1440 agacaatggt atatgcatct aagtaaatat aatgttaaag taggagatta tgtcaaagct 1500 ggtcaaataa tcggttggtc tggaagcact ggttattcta cagcaccaca tttacacttc 1560 caaagaatgg ttaattcatt ttcaaattca actgcccaag atccaatgcc tttcttaaag 1620 agcgcaggat atggaaaagc aggtggtaca gtaactccaa cgccgaatac aggttggaaa 1680 cagaataaag atggcatttg gtataaagct gaacatgctt cgttcacagt gacagcacca 1740 gagggaatta tcacaagata caaaggtcct tggactggtc acccacaagc tggtgtatta 1800 caaaaaggtc aaacgattaa atatgatgag gttcaaaaat ttgacggtca tgtttgggta 1860 tcgtgggaaa cgtttgaggg cgaaactgta tacatgccgg tacgcacatg ggacgctaaa 1920 actggtaaag ttggtaagtt gtggggcgaa attaaataa 1959 <210> 48 <211> 1554 <212> DNA <213> artificial <220> <223> His-tagged M23-LST_Ami2638_CBD2638 <400> 48 atgagaggat cgcatcacca tcaccatcac ggatccgctg caacacatga acattcagca 60 caatggttga ataattacaa aaaaggatat ggttacggtc cttatccatt aggtataaat 120 ggcggtatgc actacggagt tgattttttt atgaatattg gaacaccagt aaaagctatt 180 tcaagcggaa aaatagttga agctggttgg agtaattacg gaggaggtaa tcaaataggt 240 cttattgaaa atgatggagt gcatagacaa tggtatatgc atctaagtaa atataatgtt 300 aaagtaggag attatgtcaa agctggtcaa ataatcggtt ggtctggaag cactggttat 360 tctacagcac cacatttaca cttccaaaga atggttaatt cattttcaaa ttcaactgcc 420 caagatccaa tgcctttctt aaagagcgca ggatatggaa aagcaggtgg tacagtaact 480 ccaacgccga atacaggtga gctcttacgc cctaaagacg caaagaaaga tgaaaaatca 540 caagtatgta gtggtttggc tatggaaaaa tatgacatta caaatttaaa tgctaaacaa 600 gataaatcaa agaatgggag cgtgaaagag ttgaaacata tctattcaaa ccatattaaa 660 ggtaacaaga ttacagcacc aaaacctagt attcaaggtg tggtcatcca caatgattat 720 ggtagtatga cacctagtca atacttacca tggttatatg cacgtgagaa taacggtaca 780 cacgttaacg gttgggctag tgtttatgca aatagaaacg aagtgctttg gtatcatccg 840 acagactacg tagagtggca ttgtggtaat caatgggcaa atgctaactt aatcggattt 900 gaagtgtgtg agtcgtatcc tggtagaatc tcggacaaat tattcttaga aaatgaagaa 960 gcgacattga aagtagctgc ggatgtgatg aagtcgtacg gattaccagt taatcgcaac 1020 actgtacgtc tgcataacga attcttcgga acttcttgtc cacatcgttc gtgggacttg 1080 catgttggca aaggtgagcc ttacacaact actaatatta ataaaatgaa agactacttc 1140 atcaaacgca tcaaacatta ttatgacggt ggaaagctag aagtaagcaa agcagcaact 1200 atcaaacaat ctgacgttaa gcaagaagtt aaaaagcaag aagcaaaaca aattgtgaaa 1260 gcaacagatt ggaaacagaa taaagatggc atttggtata aagctgaaca tgcttcgttc 1320 acagtgacag caccagaggg aattatcaca agatacaaag gtccttggac tggtcaccca 1380 caagctggtg tattacaaaa aggtcaaacg attaaatatg atgaggttca aaaatttgac 1440 ggtcatgttt gggtatcgtg ggaaacgttt gagggcgaaa ctgtatacat gccggtacgc 1500 acatgggacg ctaaaactgg taaagttggt aagttgtggg gcgaaattaa ataa 1554 <210> 49 <211> 1887 <212> DNA <213> artificial <220> <223> His-tagged M23-2638_Ami2638_CBD2638_CBD2638 <400> 49 atgagaggat cgcatcacca tcaccatcac ggatccatgc taactgctat tgactatctt 60 acgaaaaaag gttggaaaat atcatctgac cctcgcactt acgatggtta ccctaaaaac 120 tacggctaca gaaattacca tgaaaacggc attaattatg atgagttttg tggtggttat 180 catagagctt ttgatgttta cagtaacgaa actaacgacg tgcctgctgt tactagcgga 240 acagttattg aagcaaacga ttacggtaat tttggtggta cattcgttat tagagacgct 300 aacgataacg attggatata tgggcatcta caacgtggct caatgcgatt tgttgtaggc 360 gacaaagtca atcaaggtga cattattggt ttacaaggta atagcaacta ttacgacaat 420 cctatgagtg tacatttaca tttacaatta cgccctaaag acgcaaagaa agatgaaaaa 480 tcacaagtat gtagtggttt ggctatggaa aaatatgaca ttacaaattt aaatgctaaa 540 caagataaat caaagaatgg gagcgtgaaa gagttgaaac atatctattc aaaccatatt 600 aaaggtaaca agattacagc accaaaacct agtattcaag gtgtggtcat ccacaatgat 660 tatggtagta tgacacctag tcaatactta ccatggttat atgcacgtga gaataacggt 720 acacacgtta acggttgggc tagtgtttat gcaaatagaa acgaagtgct ttggtatcat 780 ccgacagact acgtagagtg gcattgtggt aatcaatggg caaatgctaa cttaatcgga 840 tttgaagtgt gtgagtcgta tcctggtaga atctcggaca aattattctt agaaaatgaa 900 gaagcgacat tgaaagtagc tgcggatgtg atgaagtcgt acggattacc agttaatcgc 960 aacactgtac gtctgcataa cgaattcttc ggaacttctt gtccacatcg ttcgtgggac 1020 ttgcatgttg gcaaaggtga gccttacaca actactaata ttaataaaat gaaagactac 1080 ttcatcaaac gcatcaaaca ttattatgac ggtggaaagc tagaagtaag caaagcagca 1140 actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 1200 aaagcaacag attggaaaca gaataaagat ggcatttggt ataaagctga acatgcttcg 1260 ttcacagtga cagcaccaga gggaattatc acaagataca aaggtccttg gactggtcac 1320 ccacaagctg gtgtattaca aaaaggtcaa acgattaaat atgatgaggt tcaaaaattt 1380 gacggtcatg tttgggtatc gtgggaaacg tttgagggcg aaactgtata catgccggta 1440 cgcacatggg acgctaaaac tggtaaagtt ggtaagttgt ggggcgaaat taaagagctc 1500 ggtggaaagc tagaagtaag caaagcagca actatcaaac aatctgacgt taagcaagaa 1560 gttaaaaagc aagaagcaaa acaaattgtg aaagcaacag attggaaaca gaataaagat 1620 ggcatttggt ataaagctga acatgcttcg ttcacagtga cagcaccaga gggaattatc 1680 acaagataca aaggtccttg gactggtcac ccacaagctg gtgtattaca aaaaggtcaa 1740 acgattaaat atgatgaggt tcaaaaattt gacggtcatg tttgggtatc gtgggaaacg 1800 tttgagggcg aaactgtata catgccggta cgcacatggg acgctaaaac tggtaaagtt 1860 ggtaagttgt ggggcgaaat taaataa 1887 <210> 50 <211 >3461 <212> DNA <213> artificial <220> <223> pQE-30 vector <400> 50 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca 60 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga 120 ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct 180 gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag 240 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga 300 atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc 360 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt 420 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta 480 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc 540 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg 600 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc 660 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg 720 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc 780 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac 840 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg 900 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt 960 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt 1020 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg 1080 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1140 cctgagtagg acaaatccgc cctctagagc tgcctcgcgc gtttcggtga tgacggtgaa 1200 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 1260 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 1320 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 1380 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 1440 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 1500 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 1560 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 1620 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 1680 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1740 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 1800 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1860 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 1920 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 1980 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 2040 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 2100 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 2160 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 2220 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 2280 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 2340 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 2400 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 2460 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 2520 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 2580 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 2640 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 2700 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 2760 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 2820 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 2880 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 2940 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 3000 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 3060 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 3120 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 3180 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 3240 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 3300 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 3360 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 3420 cctataaaaa taggcgtatc acgaggccct ttcgtcttca c 3461

<210>51 <211 > 127 <212> PRT <213> Staphylococcus aureus bacteriophage 2638a <400> 51

Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val 15 10 15

Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr 20 25 30

Asp Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala 35 40 45

Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly 50 55 60

Pro Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr 65 70 75 80

Ile Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser 85 90 95

Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp 100 105 110

Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys 115 120 125

<210> 52 <211 > 106 <212> PRT <213> Staphylococcus simulans <400> 52

Gly Lys Ala Gly Gly Thr Val Thr Pro Thr Pro Asn Thr Gly Trp Lys 15 10 15

Thr Asn Lys Tyr Gly Thr Leu Tyr Lys Ser Glu Ser Ala Ser Phe Thr 20 25 30

Pro Asn Thr Asp Ile Ile Thr Arg Thr Thr Gly Pro Phe Arg Ser Met 35 40 45

Pro Gin Ser Gly Val Leu Lys Ala Gly Gin Thr Ile His Tyr Asp Glu 50 55 60

Val Met Lys Gin Asp Gly His Val Trp Val Gly Tyr Thr Gly Asn Ser 65 70 75 80

Gly Gin Arg Ile Tyr Leu Pro Val Arg Thr Trp Asn Lys Ser Thr Asn 85 90 95

Thr Leu Gly Val Leu Trp Gly Thr Ile Lys 100 105

<210> 53 <211> 190 <212> PRT <213> Staphylococcus aureus bacteriophage 2638a <400> 53

Met Leu Thr Ala Ile Asp Tyr Leu Thr Lys Lys Gly Trp Lys Ile Ser 15 10 15

Ser Asp Pro Arg Thr Tyr Asp Gly Tyr Pro Lys Asn Tyr Gly Tyr Arg 20 25 30

Asn Tyr His Glu Asn Gly lie Asn Tyr Asp Glu Phe Cys Gly Gly Tyr 35 40 45

His Arg Ala Phe Asp Val Tyr Ser Asn Glu Thr Asn Asp Val Pro Ala 50 55 60

Val Thr Ser Gly Thr Val lie Glu Ala Asn Asp Tyr Gly Asn Phe Gly 65 70 75 80

Gly Thr Phe Val lie Arg Asp Ala Asn Asp Asn Asp Trp lie Tyr Gly 85 90 95

His Leu Gin Arg Gly Ser Met Arg Phe Val Val Gly Asp Lys Val Asn 100 105 110

Gin Gly Asp lie lie Gly Leu Gin Gly Asn Ser Asn Tyr Tyr Asp Asn 115 120 125

Pro Met Ser Val His Leu His Leu Gin Leu Arg Pro Lys Asp Ala Lys 130 135 140

Lys Asp Glu Lys Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr 145 150 155 160

Asp lie Thr Asn Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser 165 170 175

Val Lys Glu Leu Lys His lie Tyr Ser Asn His lie Lys Gly 180 185 190

<210> 54 <211> 154 <212> PRT <213> Staphylococcus simulans <400> 54

Ala Ala Thr His Glu His Ser Ala Gin Trp Leu Asn Asn Tyr Lys Lys 15 10 15

Gly Tyr Gly Tyr Gly Pro Tyr Pro Leu Gly lie Asn Gly Gly Met His 20 25 30

Tyr Gly Val Asp Phe Phe Met Asn lie Gly Thr Pro Val Lys Ala lie 35 40 45

Ser Ser Gly Lys lie Val Glu Ala Gly Trp Ser Asn Tyr Gly Gly Gly 50 55 60

Asn Gin lie Gly Leu lie Glu Asn Asp Gly Val His Arg Gin Trp Tyr 65 70 75 80

Met His Leu Ser Lys Tyr Asn Val Lys Val Gly Asp Tyr Val Lys Ala 85 90 95

Gly Gin lie lie Gly Trp Ser Gly Ser Thr Gly Tyr Ser Thr Ala Pro 100 105 110

His Leu His Phe Gin Arg Met Val Asn Ser Phe Ser Asn Ser Thr Ala 115 120 125

Gin Asp Pro Met Pro Phe Leu Lys Ser Ala Gly Tyr Gly Lys Ala Gly 130 135 140

Gly Thr Val Thr Pro Thr Pro Asn Thr Gly 145 150

<210> 55 <211 > 246 <212> PRT <213> Staphylococcus aureus bacteriophage 2638a <400> 55

Glu Lys Ser Gin Val Cys Ser Gly Leu Ala Met Glu Lys Tyr Asp Ile 15 10 15

Thr Asn Leu Asn Ala Lys Gin Asp Lys Ser Lys Asn Gly Ser Val Lys 20 25 30

Glu Leu Lys His Ile Tyr Ser Asn His Ile Lys Gly Asn Lys Ile Thr 35 40 45

Ala Pro Lys Pro Ser Ile Gin Gly Val Val Ile His Asn Asp Tyr Gly 50 55 60

Ser Met Thr Pro Ser Gin Tyr Leu Pro Trp Leu Tyr Ala Arg Glu Asn 65 70 75 80

Asn Gly Thr His Val Asn Gly Trp Ala Ser Val Tyr Ala Asn Arg Asn 85 90 95

Glu Val Leu Trp Tyr His Pro Thr Asp Tyr Val Glu Trp His Cys Gly 100 105 110

Asn Gin Trp Ala Asn Ala Asn Leu Ile Gly Phe Glu Val Cys Glu Ser 115 120 125

Tyr Pro Gly Arg Ile Ser Asp Lys Leu Phe Leu Glu Asn Glu Glu Ala 130 135 140

Thr Leu Lys Val Ala Ala Asp Val Met Lys Ser Tyr Gly Leu Pro Val 145 150 155 160

Asn Arg Asn Thr Val Arg Leu His Asn Glu Phe Phe Gly Thr Ser Cys 165 170 175

Pro His Arg Ser Trp Asp Leu His Val Gly Lys Gly Glu Pro Tyr Thr 180 185 190

Thr Thr Asn Ile Asn Lys Met Lys Asp Tyr Phe Ile Lys Arg Ile Lys 195 200 205

His Tyr Tyr Asp Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile 210 215 220

Lys Gin Ser Asp Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin 225 230 235 240

Ile Val Lys Ala Thr Asp 245

<210> 56 <211 > 220 <212> PRT <213> Staphylococcus aureus phage phi 11 <400> 56

Pro Lys Lys Glu Thr Ala Lys Pro Gin Pro Lys Ala Val Glu Leu Lys 15 10 15

Ile Ile Lys Asp Val Val Lys Gly Tyr Asp Leu Pro Lys Arg Gly Ser 20 25 30

Asn Pro Lys Gly Ile Val Ile His Asn Asp Ala Gly Ser Lys Gly Ala 35 40 45

Thr Ala Glu Ala Tyr Arg Asn Gly Leu Val Asn Ala Pro Leu Ser Arg 50 55 60

Leu Glu Ala Gly Ile Ala His Ser Tyr Val Ser Gly Asn Thr Val Trp 65 70 75 80

Gin Ala Leu Asp Glu Ser Gin Val Gly Trp His Thr Ala Asn Gin Ile 85 90 95

Gly Asn Lys Tyr Tyr Tyr Gly Ile Glu Val Cys Gin Ser Met Gly Ala 100 105 110

Asp Asn Ala Thr Phe Leu Lys Asn Glu Gin Ala Thr Phe Gin Glu Cys 115 120 125

Ala Arg Leu Leu Lys Lys Trp Gly Leu Pro Ala Asn Arg Asn Thr Ile 130 135 140

Arg Leu His Asn Glu Phe Thr Ser Thr Ser Cys Pro His Arg Ser Ser 145 150 155 160

Val Leu His Thr Gly Phe Asp Pro Val Thr Arg Gly Leu Leu Pro Glu 165 170 175

Asp Lys Arg Leu Gin Leu Lys Asp Tyr Phe Ile Lys Gin Ile Arg Ala 180 185 190

Tyr Met Asp Gly Lys Ile Pro Val Ala Thr Val Ser Asn Glu Ser Ser 195 200 205

Ala Ser Ser Asn Thr Val Lys Pro Val Ala Ser Ala 210 215 220

<210> 57 <211 > 206 <212> PRT <213> Staphylococcus aureus phage phi 11 <400> 57

Met Ser Ile Ile Met Glu Val Ala Thr Met Gin Ala Lys Leu Thr Lys 15 10 15

Asn Glu Phe Ile Glu Trp Leu Lys Thr Ser Glu Gly Lys Gin Phe Asn 20 25 30

Val Asp Leu Trp Tyr Gly Phe Gin Cys Phe Asp Tyr Ala Asn Ala Gly 35 40 45

Trp Lys Val Leu Phe Gly Leu Leu Leu Lys Gly Leu Gly Ala Lys Asp 50 55 60

Ile Pro Phe Ala Asn Asn Phe Asp Gly Leu Ala Thr Val Tyr Gin Asn 65 70 75 80

Thr Pro Asp Phe Leu Ala Gin Pro Gly Asp Met Val Val Phe Gly Ser 85 90 95

Asn Tyr Gly Ala Gly Tyr Gly His Val Ala Trp Val Ile Glu Ala Thr 100 105 110

Leu Asp Tyr Ile Ile Val Tyr Glu Gin Asn Trp Leu Gly Gly Gly Trp 115 120 125

Thr Asp Gly Ile Glu Gin Pro Gly Trp Gly Trp Glu Lys Val Thr Arg 130 135 140

Arg Gin His Ala Tyr Asp Phe Pro Met Trp Phe Ile Arg Pro Asn Phe 145 150 155 160

Lys Ser Glu Thr Ala Pro Arg Ser Val Gin Ser Pro Thr Gin Ala Pro 165 170 175

Lys Lys Glu Thr Ala Lys Pro Gin Pro Lys Ala Val Glu Leu Lys Ile 180 185 190

Ile Lys Asp Val Val Lys Gly Tyr Asp Leu Pro Lys Arg Gly 195 200 205

<210> 58 <211> 187 <212> PRT <213> Staphylococcus phage Twort <400> 58

Met Lys Thr Leu Lys Gin Ala Glu Ser Tyr Ile Lys Ser Lys Val Asn 15 10 15

Thr Gly Thr Asp Phe Asp Gly Leu Tyr Gly Tyr Gin Cys Met Asp Leu 20 25 30

Ala Val Asp Tyr Ile Tyr His Val Thr Asp Gly Lys Ile Arg Met Trp 35 40 45

Gly Asn Ala Lys Asp Ala Ile Asn Asn Ser Phe Gly Gly Thr Ala Thr 50 55 60

Val Tyr Lys Asn Tyr Pro Ala Phe Arg Pro Lys Tyr Gly Asp Val Val 65 70 75 80

Val Trp Thr Thr Gly Asn Phe Ala Thr Tyr Gly His Ile Ala Ile Val 85 90 95

Thr Asn Pro Asp Pro Tyr Gly Asp Leu Gin Tyr Val Thr Val Leu Glu 100 105 110

Gin Asn Trp Asn Gly Asn Gly Ile Tyr Lys Thr Glu Leu Ala Thr Ile 115 120 125

Arg Thr His Asp Tyr Thr Gly Ile Thr His Phe Ile Arg Pro Asn Phe 130 135 140

Ala Thr Glu Ser Ser Val Lys Lys Lys Asp Thr Lys Lys Lys Pro Lys 145 150 155 160

Pro Ser Asn Arg Asp Gly Ile Asn Lys Asp Lys Ile Val Tyr Asp Arg 165 170 175

Thr Asn Ile Asn Tyr Asn Met Val Lys Arg Gly 180 185 <210> 59 <211 >4546 <212> DNA <213> artificial <220> <223> pHGFP_CBD2638_c vector <400> 59 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca 60 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga 120 ggatcgcatc accatcacca tcacggatcc atgagtaaag gagaagaact tttcactgga 180 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 240 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact 300 ggaaaactac ctgttccatg gccaacactt gtcactactt tcgcgtatgg tcttcaatgc 360 tttgcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa 420 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct 480 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 540 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 600 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac 660 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat 720 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat 780 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca 840 catggcatgg atgaactata caaagagctc ggtggaaagc tagaagtaag caaagcagca 900 actatcaaac aatctgacgt taagcaagaa gttaaaaagc aagaagcaaa acaaattgtg 960 aaagcaacag attggaaaca gaataaagat ggcatttggt ataaagctga acatgcttcg 1020 ttcacagtga cagcaccaga gggaattatc acaagataca aaggtccttg gactggtcac 1080 ccacaagctg gtgtattaca aaaaggtcaa acgattaaat atgatgaggt tcaaaaattt 1140 gacggtcatg tttgggtatc gtgggaaacg tttgagggcg aaactgtata catgccggta 1200 cgcacatggg acgctaaaac tggtaaagtt ggtaagttgt ggggcgaaat taaataagtc 1260 gacctgcagc caagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac 1320 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 1380 tgagaatcca agctagcttg gcgagatttt caggagctaa ggaagctaaa atggagaaaa 1440 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 1500 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 1560 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 1620 cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga 1680 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 1740 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 1800 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 1860 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 1920 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 1980 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa 2040 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag 2100 gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag gcatcaaata 2160 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2220 gctctcctga gtaggacaaa tccgccctct agagctgcct cgcgcgtttc ggtgatgacg 2280 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 2340 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 2400 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 2460 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 2520 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 2580 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2640 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2700 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2760 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2820 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2880 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2940 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3000 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3060 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3120 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 3180 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3240 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3300 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3360 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3420 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 3480 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 3540 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 3600 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 3660 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 3720 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 3780 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 3840 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 3900 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 3960 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 4020 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 4080 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 4140 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 4200 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 4260 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 4320 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 4380 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 4440 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 4500 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac 4546 <210> 60 <211> 1143 <212> DNA <213> artificial <220> <223> His-tagged GFP_CBD2638 <400> 60 atgagaggat cgcatcacca tcaccatcac ggatccatga gtaaaggaga agaacttttc 60 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 120 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 180 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt 240 caatgctttg cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg 300 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 360 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 420 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 480 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 540 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 600 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 660 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 720 attacacatg gcatggatga actatacaaa gagctcggtg gaaagctaga agtaagcaaa 780 gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 840 attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 900 gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 960 ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1020 aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1080 ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1140 taa 1143 <210>61 <211> 1539 <212> DNA <213> artificial <220 <223> His-tagged GFP_CBD2638_CBD2638 var.1 <400> 61 atgagaggat cgcatcacca tcaccatcac ggatccatga gtaaaggaga agaacttttc 60 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 120 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 180 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt 240 caatgctttg cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg 300 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 360 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 420 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 480 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 540 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 600 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 660 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 720 attacacatg gcatggatga actatacaaa gagctcggtg gaaagctaga agtaagcaaa 780 gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 840 attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 900 gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 960 ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1020 aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1080 ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1140 gagctcggtg gaaagctaga agtaagcaaa gcagcaacta tcaaacaatc tgacgttaag 1200 caagaagtta aaaagcaaga agcaaaacaa attgtgaaag caacagattg gaaacagaat 1260 aaagatggca tttggtataa agctgaacat gcttcgttca cagtgacagc accagaggga 1320 attatcacaa gatacaaagg tccttggact ggtcacccac aagctggtgt attacaaaaa 1380 ggtcaaacga ttaaatatga tgaggttcaa aaatttgacg gtcatgtttg ggtatcgtgg 1440 gaaacgtttg agggcgaaac tgtatacatg ccggtacgca catgggacgc taaaactggt 1500 aaagttggta agttgtgggg cgaaattaaa taagtcgac 1539 <210> 62 <211> 1560 <212> DNA <213> artificial <220> <223> Hist-tagged GFP_CBD2638_CBD2638 var. 2 <400> 62 atgagaggat cgcatcacca tcaccatcac ggatccatga gtaaaggaga agaacttttc 60 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 120 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 180 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt 240 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 300 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 360 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 420 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 480 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 540 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 600 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 660 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 720 attacacatg gcatggatga actatacaaa gagctcggtg gaaagctaga agtaagcaaa 780 gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 840 attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 900 gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 960 ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1020 aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1080 ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1140 ggtaccggtg gaaagctaga agtaagcaaa gcagcaacta tcaaacaatc tgacgttaag 1200 caagaagtta aaaagcaaga agcaaaacaa attgtgaaag caacagattg gaaacagaat 1260 aaagatggca tttggtataa agctgaacat gcttcgttca cagtgacagc accagaggga 1320 attatcacaa gatacaaagg tccttggact ggtcacccac aagctggtgt attacaaaaa 1380 ggtcaaacga ttaaatatga tgaggttcaa aaatttgacg gtcatgtttg ggtatcgtgg 1440 gaaacgtttg agggcgaaac tgtatacatg ccggtacgca catgggacgc taaaactggt 1500 aaagttggta agttgtgggg cgaaattaaa gtcgacctgc agccaagctt aattagctga 1560 <210> 63 <211> 1944 <212> DNA <213> artificial <220> <223> His-tagged GFP_CBD2638_CBD2638_CBD2638 <400> 63 atgagaggat cgcatcacca tcaccatcac ggatccatga gtaaaggaga agaacttttc 60 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 120 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 180 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt 240 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 300 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 360 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 420 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 480 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 540 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 600 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 660 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 720 attacacatg gcatggatga actatacaaa gagctcggtg gaaagctaga agtaagcaaa 780 gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 840 attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 900 gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 960 ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1020 aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1080 ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1140 ggtaccggtg gaaagctaga agtaagcaaa gcagcaacta tcaaacaatc tgacgttaag 1200 caagaagtta aaaagcaaga agcaaaacaa attgtgaaag caacagattg gaaacagaat 1260 aaagatggca tttggtataa agctgaacat gcttcgttca cagtgacagc accagaggga 1320 attatcacaa gatacaaagg tccttggact ggtcacccac aagctggtgt attacaaaaa 1380 ggtcaaacga ttaaatatga tgaggttcaa aaatttgacg gtcatgtttg ggtatcgtgg 1440 gaaacgtttg agggcgaaac tgtatacatg ccggtacgca catgggacgc taaaactggt 1500 aaagttggta agttgtgggg cgaaattaaa gtcgacggtg gaaagctaga agtaagcaaa 1560 gcagcaacta tcaaacaatc tgacgttaag caagaagtta aaaagcaaga agcaaaacaa 1620 attgtgaaag caacagattg gaaacagaat aaagatggca tttggtataa agctgaacat 1680 gcttcgttca cagtgacagc accagaggga attatcacaa gatacaaagg tccttggact 1740 ggtcacccac aagctggtgt attacaaaaa ggtcaaacga ttaaatatga tgaggttcaa 1800 aaatttgacg gtcatgtttg ggtatcgtgg gaaacgtttg agggcgaaac tgtatacatg 1860 ccggtacgca catgggacgc taaaactggt aaagttggta agttgtgggg cgaaattaaa 1920 ctgcagccaa gcttaattag ctga 1944 <210> 64 <211 > 380 <212> PRT <213> artificial <220> <223> His-tagged GFP_CBD2638 <400> 64

Met Arg Gly Ser His His His His His His Gly Ser Met Ser Lys Gly 15 10 15

Glu Glu Leu Phe Thr Gly Val Val Pro Ile Len Val Glu Leu Asp Gly 20 25 30

Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 35 40 45

Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 50 55 60

Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ala Tyr Gly Leu 65 70 75 80

Gin Cys Phe Ala Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe 85 90 95

Lys Ser Ala Met Pro Glu Gly Tyr Val Gin Glu Arg Thr Ile Phe Phe 100 105 110

Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 115 120 125

Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 130 135 140

Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 145 150 155 160

Asn Val Tyr Ile Met Ala Asp Lys Gin Lys Asn Gly Ile Lys Val Asn 165 170 175

Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gin Leu Ala Asp 180 185 190

His Tyr Gin Gin Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 195 200 205

Asp Asn His Tyr Leu Ser Thr Gin Ser Ala Leu Ser Lys Asp Pro Asn 210 215 220

Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 225 230 235 240

Ile Thr His Gly Met Asp Glu Leu Tyr Lys Glu Leu Gly Gly Lys Leu 245 250 255

Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys Gin Glu 260 265 270

Val Lys Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp Trp Lys 275 280 285

Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr 290 295 300

Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr 305 310 315 320

Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr 325 330 335

Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr 340 345 350

Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys 355 360 365

Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys 370 375 380 <210> 65 <211 > 510 <212> PRT <213> artificial <220> <223> His-tagged GFP_CBD2638_CBD2638 var.1 <400> 65

Met Arg Gly Ser His His His His His His Gly Ser Met Ser Lys Gly 15 10 15

Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly 20 25 30

Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 35 40 45

Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 50 55 60

Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ala Tyr Gly Leu 65 70 75 80

Gin Cys Phe Ala Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe 85 90 95

Lys Ser Ala Met Pro Glu Gly Tyr Val Gin Glu Arg Thr Ile Phe Phe 100 105 110

Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 115 120 125

Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 130 135 140

Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 145 150 155 160

Asn Val Tyr Ile Met Ala Asp Lys Gin Lys Asn Gly Ile Lys Val Asn 165 170 175

Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gin Leu Ala Asp 180 185 190

His Tyr Gin Gin Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 195 200 205

Asp Asn His Tyr Leu Ser Thr Gin Ser Ala Leu Ser Lys Asp Pro Asn 210 215 220

Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 225 230 235 240

Ile Thr His Gly Met Asp Glu Leu Tyr Lys Glu Leu Gly Gly Lys Leu 245 250 255

Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys Gin Glu 260 265 270

Val Lys Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp Trp Lys 275 280 285

Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr 290 295 300

Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr 305 310 315 320

Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr 325 330 335

Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr 340 345 350

Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys 355 360 365

Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Glu Leu Gly Gly 370 375 380

Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys 385 390 395 400

Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp 405 410 415

Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser 420 425 430

Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro 435 440 445

Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile 450 455 460

Lys Tyr Asp Glu val Gin Lys Phe Asp Gly His val Trp val Ser Trp 465 470 475 480

Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp 485 490 495

Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys 500 505 510 <210> 66 <211 > 519 <212> PRT <213> artificial <220> <223> His-tagged GFP_CBD2638_CBD2638 var. 2 <400> 66

Met Arg Gly Ser His His His His His His Gly Ser Met Ser Lys Gly 15 10 15

Glu Glu Leu Phe Thr Gly Val Val Pro Ile Len Val Glu Leu Asp Gly 20 25 30

Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 35 40 45

Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 50 55 60

Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ala Tyr Gly Leu 65 70 75 80

Gin Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gin His Asp Phe Phe 85 90 95

Lys Ser Ala Met Pro Glu Gly Tyr Val Gin Glu Arg Thr Ile Phe Phe 100 105 110

Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 115 120 125

Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 130 135 140

Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 145 150 155 160

Asn Val Tyr Ile Met Ala Asp Lys Gin Lys Asn Gly Ile Lys Val Asn 165 170 175

Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gin Leu Ala Asp 180 185 190

His Tyr Gin Gin Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 195 200 205

Asp Asn His Tyr Leu Ser Thr Gin Ser Ala Leu Ser Lys Asp Pro Asn 210 215 220

Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 225 230 235 240

Ile Thr His Gly Met Asp Glu Leu Tyr Lys Glu Leu Gly Gly Lys Leu 245 250 255

Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys Gin Glu 260 265 270

Val Lys Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp Trp Lys 275 280 285

Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr 290 295 300

Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr 305 310 315 320

Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr 325 330 335

Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr 340 345 350

Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys 355 360 365

Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Gly Thr Gly Gly 370 375 380

Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys 385 390 395 400

Gin Glu Val Lys Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp 405 410 415

Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser 420 425 430

Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro 435 440 445

Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile 450 455 460

Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp 465 470 475 480

Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp 485 490 495

Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Val Asp 500 505 510

Leu Gin Pro Ser Leu Ile Ser 515 <210> 67 <211 > 647 <212> PRT <213> artificial <220> <223> His-tagged GFP_CBD2638_CBD2638_CBD2638 <400> 67

Met Arg Gly Ser His His His His His His Gly Ser Met Ser Lys Gly 15 10 15

Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly 20 25 30

Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 35 40 45

Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 50 55 60

Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe Ala Tyr Gly Leu 65 70 75 80

Gin Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gin His Asp Phe Phe 85 90 95

Lys Ser Ala Met Pro Glu Gly Tyr Val Gin Glu Arg Thr Ile Phe Phe 100 105 110

Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 115 120 125

Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 130 135 140

Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 145 150 155 160

Asn Val Tyr Ile Met Ala Asp Lys Gin Lys Asn Gly Ile Lys Val Asn 165 170 175

Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gin Leu Ala Asp 180 185 190

His Tyr Gin Gin Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 195 200 205

Asp Asn His Tyr Leu Ser Thr Gin Ser Ala Leu Ser Lys Asp Pro Asn 210 215 220

Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 225 230 235 240

Ile Thr His Gly Met Asp Glu Leu Tyr Lys Glu Leu Gly Gly Lys Leu 245 250 255

Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys Gin Glu 260 265 270

Val Lys Lys Gin Glu Ala Lys Gin Ile Val Lys Ala Thr Asp Trp Lys 275 280 285

Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser Phe Thr 290 295 300

Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys Gly Pro Trp Thr 305 310 315 320

Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile Lys Tyr 325 330 335

Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp Glu Thr 340 345 350

Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp Ala Lys 355 360 365

Thr Gly Lys Val Gly Lys Leu Trp Gly Glu ile Lys Gly Thr Gly Gly 370 375 380

Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp Val Lys 385 390 395 400

Gin Glu Val Lys Lys Gin Glu Ala Lys Gin ile Val Lys Ala Thr Asp 405 410 415

Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His Ala Ser 420 425 430

Phe Thr Val Thr Ala Pro Glu Gly Ile ile Thr Arg Tyr Lys Gly Pro 435 440 445

Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin Thr Ile 450 455 460

Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val Ser Trp 465 470 475 480

Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr Trp Asp 485 490 495

Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys Val Asp 500 505 510

Gly Gly Lys Leu Glu Val Ser Lys Ala Ala Thr Ile Lys Gin Ser Asp 515 520 525

Val Lys Gin Glu Val Lys Lys Gin Glu Ala Lys Gin ile Val Lys Ala 530 535 540

Thr Asp Trp Lys Gin Asn Lys Asp Gly Ile Trp Tyr Lys Ala Glu His 545 550 555 560

Ala Ser Phe Thr Val Thr Ala Pro Glu Gly Ile Ile Thr Arg Tyr Lys 565 570 575

Gly Pro Trp Thr Gly His Pro Gin Ala Gly Val Leu Gin Lys Gly Gin 580 585 590

Thr Ile Lys Tyr Asp Glu Val Gin Lys Phe Asp Gly His Val Trp Val 595 600 605

Ser Trp Glu Thr Phe Glu Gly Glu Thr Val Tyr Met Pro Val Arg Thr 610 615 620

Trp Asp Ala Lys Thr Gly Lys Val Gly Lys Leu Trp Gly Glu Ile Lys 625 630 635 640

Leu Gin Pro Ser Leu ile Ser 645

REFERENCES CITED IN THE DESCRIPTION

This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Non-patent literature cited in the description • LU et al.J. Biol. Chem., 2006, vol. 281, 1549-58 fSSOS] • LOESSNER et al.Molecular Microbiology, 2002, vol. 44, 2335-349 Γ00111 • TRAYER, H. R.BUCKLEY, C. E. Molecular properties of lysostaphin, a bacteriolytic agent specific for Staphylococcus aureusJ. Biol. Chem., 1970, vol. 245, 4842-4846 ΓΟ019;

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Claims (16)

1

1. Nukleinsyremolekyle omfattende en første nukleotidsekvens, hvor nu-kleotidsekvensen har mindst 85% sekvensidentitet med SEQ ID NO: 12, 5 hvor den første nukleotidsekvens koder for et cellevægbindingsdomæne, der binder peptidoglycan-cellevæggen af Staphylococcus-slægter, og hvor nu-kleinsyremolekylet yderligere omfatter en heterolog nukleotidsekvens, der koder for et lytisk domæne, som udviser peptidoglycan-hydrolase-aktivitet.

2. Nukleinsyremolekyle ifølge krav 1, hvor nukleinsyremolekylet har mindst 80 % sekvensidentitet med SEQ ID NO: 1.

3. Nukleinsyremolekyle ifølge krav 1 eller 2, hvor det lytiske domæne er en anden og tredje nukleotidsekvens, og hvor den anden nukleotidsekvens ko- 15 der for et M23-endopeptidasedomæne, og den tredje nukleotidsekvens koder for et amidasedomæne, fortrinsvis hvor den anden og tredje nukleotidsekvens stammer fra et gen, der koder for et enzym udvalgt fra gruppen bestående af S. aureus-bakteriofag <t>2638a-endolysin, S. aureus-bacteriofag Φ11-endolysin, S. aureus- bakte ri ofag (bTwort-endolysin og S. simulans-20 lysostaphin.

4. Nukleinsyremolekyle ifølge krav 3, hvor den anden nukleotidsekvens har mindst 80% sekvensidentitet med SEQ ID NO: 14 eller 15, og den tredje nukleotidsekvens har mindst 80 % sekvensidentitet med SEQ ID NO: 16 eller 17, fortrinsvis hvor nukleinsyremolekylet har mindst 80% sekvensidentitet med SEQ ID NO: 9.

5. Nukleinsyremolekyle ifølge krav 3 eller 4, yderligere omfattende en fjerde nukleotidsekvens, der koder for et CHAP (cystein, histidin-afhængige 30 amidohydrolaser/peptidaser)-domæne, fortrinsvis hvor den fjerde nu kleotidsekvens stammer fra S. aureus-bakteriofag Φ11 eller S. aureus-bakteriofag ΦTwort-endolysin, mere fortrinsvis hvor den fjerde nukleotidsekvens har mindst 80 % sekvensidentitet med SEQ ID NO: 18 eller SEQ ID NO: 19. 35 2

6. Nukleinsyremolekyle ifølge et hvilket som helst af kravene 3 - 5, som koder for et polypeptid, der har den samme eller en øget lytisk aktivitet og/eller den samme eller et reduceret pH-optimum sammenlignet med S. aureus-bakteriofag <t>2638a-endolysin, der kodes af SEQ ID NO: 1. 5

7. Polypeptid, der kodes af et nukleinsyremolekyle ifølge et hvilket som helst af kravene 1-6, omfattende: - et cellevægbindingsdomæne og - et endopeptidasedomæne og/eller et amidasedomæne. 10

8. Nukleinsyrekonstrukt omfattende et nukleinsyremolekyle ifølge et hvilket som helst af kravene 1 - 6.

9. Ekspressionsvektor omfattende et nukleinsyrekonstrukt ifølge krav 8, funk- 15 tionsmæssigt forbundet med en eller flere kontrolsekvenser, som styrer pro duktionen eller ekspressionen af det kodede polypeptid i en celle, et individ eller et cellefrit ekspressionssystem.

10. Celle omfattende nukleinsyrekonstruktet ifølge krav 8 eller ekspressions- 20 vektoren ifølge krav 9, hvilken celle er en mikrobiel, prokaryotisk eller euka- ryotisk celle.

11. Fremgangsmåde til fremstilling, eventuelt oprensning og eventuelt frysetørring af et polypeptid, der kodes af et nukleinsyremolekyle omfattende en 25 første nukleotidsekvens, hvor nukleotidsekvensen har mindst 85 % sekvensidentitet med SEQ ID NO: 12, hvor den første nukleotidsekvens koder for et cellevægbindingsdomæne, der binder peptidoglycan-cellevæggen af Staphylococcus-s\æg\.er, hvilken fremgangsmåde omfatter trinnene: i) at fremstille polypeptidet i en celle ifølge krav 10, omfattende et nukleinsy- 30 rekonstrukt ifølge krav 8, eventuelt ii) at oprense polypeptidet og eventuelt iii) at frysetørre det oprensede polypeptid.

12. Fremgangsmåde til fremstilling af et polypeptid med en forstærket lytisk 35 aktivitet ved behandling af et polypeptid ifølge krav 7, hvor fremgangsmåden omfatter trinnene: 3 i) at dialysere polypeptidet mod en buffer omfattende en chelaterende forbindelse, ii) at dialysere polypeptidet mod en divalent metalionholdig buffer, idet den divalente metalion fortrinsvis er udvalgt fra gruppen bestående af Co2+, Cu2+, 5 Mn2+ og Zn2+.

13. Sammensætning omfattende et nukleinsyremolekyle ifølge et hvilket som helst af kravene 1 - 6, et polypeptid ifølge krav 7 og/eller et nukleinsyrekon-strukt ifølge krav 8 og/eller en vektor ifølge krav 9 og/eller en celle ifølge krav 10, fortrinsvis yderligere omfattende en eller flere aktive bestanddele, for trinsvis udvalgt fra gruppen bestående af en bakteriofag og et antibiotikum.

14. Sammensætning ifølge krav 13 til anvendelse som et medikament, fortrinsvis til anvendelse som et medikament til behandling af en infektionssyg- 15 dom.

15. In vitro-anvendelse af et nukleinsyremolekyle ifølge et hvilket som helst af kravene 1 - 6, et polypeptid ifølge krav 7, et nukleinsyrekonstrukt ifølge krav 8, en vektor ifølge krav 9, en celle ifølge krav 10 og/eller en sammensætning 20 ifølge krav 13 som et antimikrobielt middel, fortrinsvis som et fødevaretilsæt ningsstof eller et desinfektionsmiddel.

16. In v/Yro-anvendelse af et nukleinsyremolekyle ifølge et hvilket som helst af kravene 1 - 6, et polypeptid ifølge krav 7, et nukleinsyrekonstrukt ifølge krav 8, en vektor ifølge krav 9, en celle ifølge krav 10 og/eller en sammensætning ifølge krav 13 til detektering af en Staphylococcus, fortrinsvis i en diagnostisk anvendelse.