DK2300799T3 - Fremgangsmåde til histokemisk forarbejdning og anvendelse af en sammensætning til histokemisk forarbejdning - Google Patents
Fremgangsmåde til histokemisk forarbejdning og anvendelse af en sammensætning til histokemisk forarbejdning Download PDFInfo
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- DK2300799T3 DK2300799T3 DK09759174.7T DK09759174T DK2300799T3 DK 2300799 T3 DK2300799 T3 DK 2300799T3 DK 09759174 T DK09759174 T DK 09759174T DK 2300799 T3 DK2300799 T3 DK 2300799T3
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- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
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- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000000562 secondary electron spectroscopy Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 235000011008 sodium phosphates Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- QJPCTSZFFFXUGR-UHFFFAOYSA-K tetrasodium;2-hydroxypropane-1,2,3-tricarboxylate;dihydrate Chemical compound O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QJPCTSZFFFXUGR-UHFFFAOYSA-K 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 235000019263 trisodium citrate Nutrition 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Nanotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Materials Engineering (AREA)
- Crystallography & Structural Chemistry (AREA)
- Composite Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Claims (12)
1. Fremgangsmåde til forarbejdning af en biologisk prøve, omfattende: tilvejebringelse af en biologisk prøve; anvende en vandig histokemisk forarbejdningssammensætning til prøven, idet sammensætningen omfatter fra mere end nul til 25 dele pr. million af mindst én nanopartikel valgt fra aluminiumoxid (AI2O3), siliciumoxid (S1O2), titaniumoxid (T1O2), eller kombinationer deraf, i en mængde der er effektiv til at reducere gennemsnitsantallet af områder af reduceret farvningsintensitet pr. objektglas; farve den biologiske prøve, og udføre mindst ét yderligere histokemisk trin på prøven.
2. Fremgangsmåden ifølge krav 1, hvor udførelsen af en histokemisk forarbejdning omfatter udførelse af cellekonditionering på prøven under anvendelse af en cellekonditioneringssammensætning.
3. Fremgangsmåden ifølge krav 1, hvor den biologiske prøve og den histokemiske forarbejdningssammensætning opvarmes til en temperatur på fra 60°C til 101°C.
4. Fremgangsmåden ifølge krav 1, hvor sammensætningen har en nanopartikelkoncentration på fra 2 dele pr. million til 20 dele pr. million.
5. Fremgangsmåden ifølge krav 1 hvor sammensætningen har en nanopartikelkoncentration på fra 2,5 dele pr. million til 15 dele pr. million.
6. Anvendelse afen vandig sammensætning omfattende mere end nul og mindre end 25 dele pr. million af mindst én nanopartikel valgt fra aluminiumoxid (AI2O3), siliciumoxid (S1O2), titaniumoxid (T1O2), og kombinationer deraf, til at udføre en histokemisk forarbejdning omfattende et farvningstrin for at reducere gennemsnitsantallet af områder af reduceret farvningsintensitet pr. objektglas.
7. Anvendelse ifølge krav 6, hvor sammensætningen omfatter et cellekonditioneringsmiddel.
8. Anvendelse ifølge krav 7, hvor den histokemiske forarbejdning udføres på en biologisk prøve omfattende en nukleinsyre, og hvor den cellekonditionerende sammensætning omfatter en opløsning af EDTA, SSC, citronsyre, eller en kombination deraf.
9. Anvendelse ifølge krav 8, hvor nukleinsyren er DNA og den cellekonditionerende sammensætning omfatter EDTA.
10. Anvendelse ifølge krav 8, hvor nukleinsyren er RNA og den cellekonditionerende prøve omfatter SSC.
11. Anvendelse ifølge krav 7, hvor den histokemiske forarbejdning udføres på en biologisk prøve omfattende et protein og den cellekonditionerende sammensætning omfatter en phosphatbuffer, en borsyrebufferopløsning, urinstof, eller en kombination deraf.
12. Anvendelse ifølge krav 6, hvor sammensætningen omfatter Tris borat-ethylendiamin-tetraeddikesyre (EDTA)-buffer, ved en pH på ca. 8.
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PCT/US2009/045841 WO2009149013A2 (en) | 2008-06-05 | 2009-06-01 | Compositions comprising nanomaterials and method for using such compositions for histochemical processes |
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DK09759174.7T DK2300799T3 (da) | 2008-06-05 | 2009-06-01 | Fremgangsmåde til histokemisk forarbejdning og anvendelse af en sammensætning til histokemisk forarbejdning |
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DK15189792.3T DK2995925T3 (da) | 2008-06-05 | 2009-06-01 | Sammensætning til histokemisk processering |
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EP (2) | EP2995925B1 (da) |
AU (1) | AU2009256349B2 (da) |
CA (2) | CA2979353C (da) |
DK (2) | DK2995925T3 (da) |
ES (2) | ES2557594T3 (da) |
WO (1) | WO2009149013A2 (da) |
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CN102372544A (zh) * | 2010-08-23 | 2012-03-14 | 中国石油化工股份有限公司 | 乙醇催化脱水的方法 |
CN103834397B (zh) * | 2014-03-11 | 2015-07-08 | 太原理工大学 | 一种水溶性荧光碳点的制备方法 |
US9145492B1 (en) * | 2014-04-01 | 2015-09-29 | King Fahd University Of Petroleum And Minerals | Method to produce ultra-high molecular weight polyethylene |
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KR101826558B1 (ko) * | 2016-02-03 | 2018-02-07 | 현대자동차 주식회사 | 산화 그래핀-나노 다이아몬드 결합체, 이의 제조 방법, 및 이를 포함하는 나노 유체 |
CN108436077B (zh) * | 2018-03-30 | 2019-10-01 | 河北工业大学 | 一种添加有纳米TiO2的合金粉末的制备方法 |
CN112830471B (zh) * | 2021-01-11 | 2022-05-10 | 大连理工大学盘锦产业技术研究院 | 一种超级电容器用二维氮掺杂多孔炭材料的制备方法 |
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EP2300799B1 (en) | 2015-11-25 |
CA2720728C (en) | 2018-04-03 |
CA2979353A1 (en) | 2009-12-10 |
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ES2557594T3 (es) | 2016-01-27 |
WO2009149013A2 (en) | 2009-12-10 |
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US20140178929A1 (en) | 2014-06-26 |
EP2995925A1 (en) | 2016-03-16 |
AU2009256349A1 (en) | 2009-12-10 |
ES2906426T3 (es) | 2022-04-18 |
WO2009149013A3 (en) | 2010-02-04 |
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