DK2032698T3 - Systematisk bedømmelse af sekvens- og aktivitetsforbindelse ved anvendelse af stedbedømmelsesbiblioteker til manipulation af flere egenskaber - Google Patents

Systematisk bedømmelse af sekvens- og aktivitetsforbindelse ved anvendelse af stedbedømmelsesbiblioteker til manipulation af flere egenskaber

Info

Publication number
DK2032698T3
DK2032698T3 DK07796357.7T DK07796357T DK2032698T3 DK 2032698 T3 DK2032698 T3 DK 2032698T3 DK 07796357 T DK07796357 T DK 07796357T DK 2032698 T3 DK2032698 T3 DK 2032698T3
Authority
DK
Denmark
Prior art keywords
assessment
libraries
connectivity
activity
sequence
Prior art date
Application number
DK07796357.7T
Other languages
English (en)
Inventor
Wolfgang Aehle
David A Estell
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38659837&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2032698(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of DK2032698T3 publication Critical patent/DK2032698T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK07796357.7T 2006-06-23 2007-06-22 Systematisk bedømmelse af sekvens- og aktivitetsforbindelse ved anvendelse af stedbedømmelsesbiblioteker til manipulation af flere egenskaber DK2032698T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81620206P 2006-06-23 2006-06-23
US93331207P 2007-06-06 2007-06-06
PCT/US2007/014556 WO2008002472A2 (en) 2006-06-23 2007-06-22 Systematic evaluation of sequence and activity relationships using site evaluation libraries for engineering multiple properties

Publications (1)

Publication Number Publication Date
DK2032698T3 true DK2032698T3 (da) 2012-09-03

Family

ID=38659837

Family Applications (1)

Application Number Title Priority Date Filing Date
DK07796357.7T DK2032698T3 (da) 2006-06-23 2007-06-22 Systematisk bedømmelse af sekvens- og aktivitetsforbindelse ved anvendelse af stedbedømmelsesbiblioteker til manipulation af flere egenskaber

Country Status (12)

Country Link
US (3) US20080004186A1 (da)
EP (1) EP2032698B1 (da)
JP (1) JP5785686B2 (da)
KR (1) KR101486087B1 (da)
CN (1) CN101473036B (da)
CA (1) CA2654269C (da)
DK (1) DK2032698T3 (da)
ES (1) ES2388753T3 (da)
HK (1) HK1128934A1 (da)
PL (1) PL2032698T3 (da)
PT (1) PT2032698E (da)
WO (1) WO2008002472A2 (da)

Families Citing this family (20)

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Publication number Priority date Publication date Assignee Title
US7498158B2 (en) 2001-05-15 2009-03-03 Novozymes A/S Alpha-amylase variant with altered properties
US7985569B2 (en) * 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
BRPI0812206A2 (pt) * 2007-06-06 2014-10-14 Danisco Us Inc Genencor Div Métodos para melhorar o desempenho de proteínas
JP2011518564A (ja) 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体
CA2726265A1 (en) * 2008-06-06 2009-12-10 Danisco Us Inc. Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties
ES2538406T3 (es) 2008-06-06 2015-06-19 Danisco Us Inc. Alfa-amilasas variantes de Bacillus subtilis y sus métodos de uso
CA2757343A1 (en) * 2009-04-01 2010-10-07 Danisco Us Inc. Compositions and methods comprising alpha-amylase variants with altered properties
WO2010124146A2 (en) 2009-04-23 2010-10-28 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
MX2012006616A (es) 2009-12-09 2012-06-21 Procter & Gamble Productos para el cuidado de las telas y el hogar.
ES2561553T3 (es) 2010-07-22 2016-02-26 Unilever N.V. Combinaciones de ramnolípidos y enzimas para una limpieza mejorada
US9273298B2 (en) 2010-10-27 2016-03-01 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
WO2012071458A1 (en) 2010-11-22 2012-05-31 Array Biopharma Inc. Heterocyclic inhibitors of beta - secretase for the treatment of neurodegenerative diseases
WO2012110564A1 (en) * 2011-02-16 2012-08-23 Novozymes A/S Detergent compositions comprising m7 or m35 metalloproteases
MX353621B (es) 2011-06-30 2018-01-22 Novozymes As Variantes de alfa-amilasa.
DE102011118027A1 (de) * 2011-09-12 2013-03-14 Henkel Ag & Co. Kgaa Verfahren zur Anpassung eines Hydrolytischen Enzyms an eine das hydrolytische Enzym stabilisierende Komponente
ES2644007T3 (es) 2012-01-26 2017-11-27 Novozymes A/S Uso de polipéptidos con actividad de proteasa en piensos para animales y en detergentes
JP6067409B2 (ja) 2012-04-10 2017-01-25 花王株式会社 アルカリプロテアーゼの溶解性向上方法
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
EP2863759A2 (en) * 2012-06-20 2015-04-29 Novozymes Biopolymer A/S Use of polypeptides having protease activity in animal feed and detergents
WO2014122161A2 (en) 2013-02-06 2014-08-14 Novozymes A/S Polypeptides having protease activity

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US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5700676A (en) * 1984-05-29 1997-12-23 Genencor International Inc. Modified subtilisins having amino acid alterations
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5322770A (en) * 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5830837A (en) * 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
EP1921148B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
US6355255B1 (en) * 1998-12-07 2002-03-12 Regents Of The University Of Minnesota Streptococcal C5a peptidase vaccine
MA25044A1 (fr) * 1997-10-23 2000-10-01 Procter & Gamble Compositions de lavage contenant des variants de proteases multisubstituees.
EP1032655B1 (en) * 1997-11-21 2005-06-29 Novozymes A/S Protease variants and compositions
JP2002500019A (ja) 1997-12-24 2002-01-08 ジェネンコア インターナショナル インコーポレーテッド 好ましい酵素および／または好ましい洗剤組成物についての改良された分析方法
US6376450B1 (en) * 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
US6933140B1 (en) * 1999-11-05 2005-08-23 Genencor International, Inc. Enzymes useful for changing the properties of polyester
US6582914B1 (en) * 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
DE10129187A1 (de) * 2001-06-19 2003-01-02 Studiengesellschaft Kohle Mbh Optimierung von synthetischen Katalysatoren durch gerichtete Evolution
JP4703113B2 (ja) * 2002-01-16 2011-06-15 ジェネンコー・インターナショナル・インク 複数置換プロテアーゼ変異体
CA2531494A1 (en) * 2003-07-11 2005-01-20 Novozymes A/S Method of screening for improved specific activity of enzymes
CA2544076A1 (en) * 2003-10-22 2005-05-06 Nascacell Ip Gmbh Method for the identification of enzymes having desired properties by immobilisation of the reaction product on the surface of enzyme-presenting organisms
US7985569B2 (en) * 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
AU2004293826B2 (en) 2003-11-19 2009-09-17 Danisco Us Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
EP2664670B1 (en) * 2003-12-03 2015-05-06 Danisco US Inc. Perhydrolase
US20060040327A1 (en) * 2004-08-18 2006-02-23 Terry Amiss Methods of screening proteins
KR20080066921A (ko) * 2005-10-12 2008-07-17 제넨코 인터내셔날 인코포레이티드 저장-안정성 중성 메탈로프로테아제의 용도 및 제조

Also Published As

Publication number Publication date
JP5785686B2 (ja) 2015-09-30
WO2008002472A3 (en) 2008-03-13
CA2654269C (en) 2015-09-22
US20140187440A1 (en) 2014-07-03
CN101473036B (zh) 2012-12-12
CN101473036A (zh) 2009-07-01
KR20090023408A (ko) 2009-03-04
WO2008002472A2 (en) 2008-01-03
US8648015B2 (en) 2014-02-11
US20080004186A1 (en) 2008-01-03
ES2388753T3 (es) 2012-10-18
EP2032698A2 (en) 2009-03-11
CA2654269A1 (en) 2008-12-03
KR101486087B1 (ko) 2015-01-26
HK1128934A1 (en) 2009-11-13
PL2032698T3 (pl) 2012-11-30
US20110082048A1 (en) 2011-04-07
JP2009540862A (ja) 2009-11-26
EP2032698B1 (en) 2012-06-27
PT2032698E (pt) 2012-09-24

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