DK2032698T3 - Systematisk bedømmelse af sekvens- og aktivitetsforbindelse ved anvendelse af stedbedømmelsesbiblioteker til manipulation af flere egenskaber - Google Patents
Systematisk bedømmelse af sekvens- og aktivitetsforbindelse ved anvendelse af stedbedømmelsesbiblioteker til manipulation af flere egenskaberInfo
- Publication number
- DK2032698T3 DK2032698T3 DK07796357.7T DK07796357T DK2032698T3 DK 2032698 T3 DK2032698 T3 DK 2032698T3 DK 07796357 T DK07796357 T DK 07796357T DK 2032698 T3 DK2032698 T3 DK 2032698T3
- Authority
- DK
- Denmark
- Prior art keywords
- assessment
- libraries
- connectivity
- activity
- sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US81620206P | 2006-06-23 | 2006-06-23 | |
US93331207P | 2007-06-06 | 2007-06-06 | |
PCT/US2007/014556 WO2008002472A2 (en) | 2006-06-23 | 2007-06-22 | Systematic evaluation of sequence and activity relationships using site evaluation libraries for engineering multiple properties |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2032698T3 true DK2032698T3 (da) | 2012-09-03 |
Family
ID=38659837
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK07796357.7T DK2032698T3 (da) | 2006-06-23 | 2007-06-22 | Systematisk bedømmelse af sekvens- og aktivitetsforbindelse ved anvendelse af stedbedømmelsesbiblioteker til manipulation af flere egenskaber |
Country Status (12)
Country | Link |
---|---|
US (3) | US20080004186A1 (da) |
EP (1) | EP2032698B1 (da) |
JP (1) | JP5785686B2 (da) |
KR (1) | KR101486087B1 (da) |
CN (1) | CN101473036B (da) |
CA (1) | CA2654269C (da) |
DK (1) | DK2032698T3 (da) |
ES (1) | ES2388753T3 (da) |
HK (1) | HK1128934A1 (da) |
PL (1) | PL2032698T3 (da) |
PT (1) | PT2032698E (da) |
WO (1) | WO2008002472A2 (da) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7498158B2 (en) | 2001-05-15 | 2009-03-03 | Novozymes A/S | Alpha-amylase variant with altered properties |
US7985569B2 (en) * | 2003-11-19 | 2011-07-26 | Danisco Us Inc. | Cellulomonas 69B4 serine protease variants |
BRPI0812206A2 (pt) * | 2007-06-06 | 2014-10-14 | Danisco Us Inc Genencor Div | Métodos para melhorar o desempenho de proteínas |
JP2011518564A (ja) | 2008-04-23 | 2011-06-30 | ダニスコ・ユーエス・インク | 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体 |
CA2726265A1 (en) * | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties |
ES2538406T3 (es) | 2008-06-06 | 2015-06-19 | Danisco Us Inc. | Alfa-amilasas variantes de Bacillus subtilis y sus métodos de uso |
CA2757343A1 (en) * | 2009-04-01 | 2010-10-07 | Danisco Us Inc. | Compositions and methods comprising alpha-amylase variants with altered properties |
WO2010124146A2 (en) | 2009-04-23 | 2010-10-28 | Danisco Us Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
MX2012006616A (es) | 2009-12-09 | 2012-06-21 | Procter & Gamble | Productos para el cuidado de las telas y el hogar. |
ES2561553T3 (es) | 2010-07-22 | 2016-02-26 | Unilever N.V. | Combinaciones de ramnolípidos y enzimas para una limpieza mejorada |
US9273298B2 (en) | 2010-10-27 | 2016-03-01 | Danisco Us Inc. | Isoprene synthase variants for improved production of isoprene |
WO2012071458A1 (en) | 2010-11-22 | 2012-05-31 | Array Biopharma Inc. | Heterocyclic inhibitors of beta - secretase for the treatment of neurodegenerative diseases |
WO2012110564A1 (en) * | 2011-02-16 | 2012-08-23 | Novozymes A/S | Detergent compositions comprising m7 or m35 metalloproteases |
MX353621B (es) | 2011-06-30 | 2018-01-22 | Novozymes As | Variantes de alfa-amilasa. |
DE102011118027A1 (de) * | 2011-09-12 | 2013-03-14 | Henkel Ag & Co. Kgaa | Verfahren zur Anpassung eines Hydrolytischen Enzyms an eine das hydrolytische Enzym stabilisierende Komponente |
ES2644007T3 (es) | 2012-01-26 | 2017-11-27 | Novozymes A/S | Uso de polipéptidos con actividad de proteasa en piensos para animales y en detergentes |
JP6067409B2 (ja) | 2012-04-10 | 2017-01-25 | 花王株式会社 | アルカリプロテアーゼの溶解性向上方法 |
US9163263B2 (en) | 2012-05-02 | 2015-10-20 | The Goodyear Tire & Rubber Company | Identification of isoprene synthase variants with improved properties for the production of isoprene |
EP2863759A2 (en) * | 2012-06-20 | 2015-04-29 | Novozymes Biopolymer A/S | Use of polypeptides having protease activity in animal feed and detergents |
WO2014122161A2 (en) | 2013-02-06 | 2014-08-14 | Novozymes A/S | Polypeptides having protease activity |
Family Cites Families (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US5972682A (en) * | 1984-05-29 | 1999-10-26 | Genencor International, Inc. | Enzymatically active modified subtilisins |
US5700676A (en) * | 1984-05-29 | 1997-12-23 | Genencor International Inc. | Modified subtilisins having amino acid alterations |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5322770A (en) * | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
US5340735A (en) | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
US5830837A (en) * | 1994-11-22 | 1998-11-03 | Novo Nordisk A/S | Amylase variants |
EP1921148B1 (en) | 1994-02-24 | 2011-06-08 | Henkel AG & Co. KGaA | Improved enzymes and detergents containing them |
US6682924B1 (en) * | 1995-05-05 | 2004-01-27 | Novozymes A/S | Protease variants and compositions |
DE19530816A1 (de) | 1995-08-23 | 1997-02-27 | Cognis Bio Umwelt | Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten |
US6355255B1 (en) * | 1998-12-07 | 2002-03-12 | Regents Of The University Of Minnesota | Streptococcal C5a peptidase vaccine |
MA25044A1 (fr) * | 1997-10-23 | 2000-10-01 | Procter & Gamble | Compositions de lavage contenant des variants de proteases multisubstituees. |
EP1032655B1 (en) * | 1997-11-21 | 2005-06-29 | Novozymes A/S | Protease variants and compositions |
JP2002500019A (ja) | 1997-12-24 | 2002-01-08 | ジェネンコア インターナショナル インコーポレーテッド | 好ましい酵素および/または好ましい洗剤組成物についての改良された分析方法 |
US6376450B1 (en) * | 1998-10-23 | 2002-04-23 | Chanchal Kumar Ghosh | Cleaning compositions containing multiply-substituted protease variants |
US6933140B1 (en) * | 1999-11-05 | 2005-08-23 | Genencor International, Inc. | Enzymes useful for changing the properties of polyester |
US6582914B1 (en) * | 2000-10-26 | 2003-06-24 | Genencor International, Inc. | Method for generating a library of oligonucleotides comprising a controlled distribution of mutations |
DE10129187A1 (de) * | 2001-06-19 | 2003-01-02 | Studiengesellschaft Kohle Mbh | Optimierung von synthetischen Katalysatoren durch gerichtete Evolution |
JP4703113B2 (ja) * | 2002-01-16 | 2011-06-15 | ジェネンコー・インターナショナル・インク | 複数置換プロテアーゼ変異体 |
CA2531494A1 (en) * | 2003-07-11 | 2005-01-20 | Novozymes A/S | Method of screening for improved specific activity of enzymes |
CA2544076A1 (en) * | 2003-10-22 | 2005-05-06 | Nascacell Ip Gmbh | Method for the identification of enzymes having desired properties by immobilisation of the reaction product on the surface of enzyme-presenting organisms |
US7985569B2 (en) * | 2003-11-19 | 2011-07-26 | Danisco Us Inc. | Cellulomonas 69B4 serine protease variants |
AU2004293826B2 (en) | 2003-11-19 | 2009-09-17 | Danisco Us Inc. | Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same |
EP2664670B1 (en) * | 2003-12-03 | 2015-05-06 | Danisco US Inc. | Perhydrolase |
US20060040327A1 (en) * | 2004-08-18 | 2006-02-23 | Terry Amiss | Methods of screening proteins |
KR20080066921A (ko) * | 2005-10-12 | 2008-07-17 | 제넨코 인터내셔날 인코포레이티드 | 저장-안정성 중성 메탈로프로테아제의 용도 및 제조 |
-
2007
- 2007-06-22 CA CA2654269A patent/CA2654269C/en active Active
- 2007-06-22 JP JP2009518173A patent/JP5785686B2/ja not_active Expired - Fee Related
- 2007-06-22 ES ES07796357T patent/ES2388753T3/es active Active
- 2007-06-22 WO PCT/US2007/014556 patent/WO2008002472A2/en active Application Filing
- 2007-06-22 KR KR1020087031336A patent/KR101486087B1/ko not_active IP Right Cessation
- 2007-06-22 PT PT07796357T patent/PT2032698E/pt unknown
- 2007-06-22 EP EP07796357A patent/EP2032698B1/en not_active Revoked
- 2007-06-22 PL PL07796357T patent/PL2032698T3/pl unknown
- 2007-06-22 US US11/821,468 patent/US20080004186A1/en not_active Abandoned
- 2007-06-22 CN CN2007800232384A patent/CN101473036B/zh active Active
- 2007-06-22 DK DK07796357.7T patent/DK2032698T3/da active
-
2009
- 2009-09-11 HK HK09108365.1A patent/HK1128934A1/xx unknown
-
2010
- 2010-05-12 US US12/778,915 patent/US8648015B2/en active Active
-
2013
- 2013-12-20 US US14/137,539 patent/US20140187440A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JP5785686B2 (ja) | 2015-09-30 |
WO2008002472A3 (en) | 2008-03-13 |
CA2654269C (en) | 2015-09-22 |
US20140187440A1 (en) | 2014-07-03 |
CN101473036B (zh) | 2012-12-12 |
CN101473036A (zh) | 2009-07-01 |
KR20090023408A (ko) | 2009-03-04 |
WO2008002472A2 (en) | 2008-01-03 |
US8648015B2 (en) | 2014-02-11 |
US20080004186A1 (en) | 2008-01-03 |
ES2388753T3 (es) | 2012-10-18 |
EP2032698A2 (en) | 2009-03-11 |
CA2654269A1 (en) | 2008-12-03 |
KR101486087B1 (ko) | 2015-01-26 |
HK1128934A1 (en) | 2009-11-13 |
PL2032698T3 (pl) | 2012-11-30 |
US20110082048A1 (en) | 2011-04-07 |
JP2009540862A (ja) | 2009-11-26 |
EP2032698B1 (en) | 2012-06-27 |
PT2032698E (pt) | 2012-09-24 |
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